ES2187664T3 - Crioconservacion de diversas celulas vegetales. - Google Patents

Crioconservacion de diversas celulas vegetales.

Info

Publication number
ES2187664T3
ES2187664T3 ES96923280T ES96923280T ES2187664T3 ES 2187664 T3 ES2187664 T3 ES 2187664T3 ES 96923280 T ES96923280 T ES 96923280T ES 96923280 T ES96923280 T ES 96923280T ES 2187664 T3 ES2187664 T3 ES 2187664T3
Authority
ES
Spain
Prior art keywords
cells
crioconservation
vegetable
crioprotector
agent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96923280T
Other languages
English (en)
Inventor
Prakash G Kadkade
Christopher B Bare
Barbara Schnabel-Preikstas
Bin Yu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Phyton Inc
Original Assignee
Phyton Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Phyton Inc filed Critical Phyton Inc
Application granted granted Critical
Publication of ES2187664T3 publication Critical patent/ES2187664T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

LA PRESENTE INVENCION TRATA DE PROCEDIMIENTOS PARA LA CRIOCONSERVACION DE CELULAS VEGETALES Y DE PROCEDIMIENTOS PARA RECUPERAR CELULAS VEGETALES VIABLES PARA UNA CRIOCONSERVACION A CORTO O LARGO PLAZO. LAS CELULAS VEGETALES QUE SE VAN A CRIOCONSERVAR PUEDEN SER CULTIVADAS EN UN CULTIVO Y PRETRATADAS CON UNA SOLUCION QUE CONTIENE UN AGENTE CRIOPROTECTOR, Y OPCIONALMENTE UN ESTABILIZANTE. LOS ESTABILIZANTES SON PREFERIBLEMENTE ESTABILIZANTES DE MEMBRANA, TALES COMO LOS INHIBIDORES DEL ETILENO, LOS CAPTADORES DE RADICALES DE OXIGENO, Y CATIONES DIVALENTES. LAS CELULAS TAMBIEN PUEDEN ESTABILIZARSE SOMETIENDO AL CULTIVO A UN CHOQUE DE CALOR. LAS CELULAS PRETRATADAS SE ACLIMATAN A UNA TEMPERATURA REDUCIDA Y SE CARGAN CON UN AGENTE CRIOPROTECTOR, TAL COMO DMSO, PROPILENGLICOL O POLIETILENGLICOL. LAS CELULAS CARGADAS SE INCUBAN CON UNA SOLUCION DE VITRIFICACION QUE INCLUYE, POR EJEMPLO, UNA SOLUCION CON UNA ELEVADA CONCENTRACION DEL AGENTE CRIOPROTECTOR. LAS CELULAS VITRIFICADAS RETIENEN MENOS DE UN 20 % DE CONTENIDO EN AGUA Y PUEDEN CONGELARSE A TEMPERATURAS DE CRIOCONSERVACION DURANTE LARGOS PERIODOS DE TIEMPO SIN QUE SE ALTEREN DE FORMA SIGNIFICATIVA EL CARACTER GENOTIPICO O FENOTIPICO DE LAS CELULAS. LAS CELULAS VEGETALES TAMBIEN PUEDEN CRIOCONSERVARSE MEDIANTE LA LIOFILIZACION DE LAS CELULAS ANTES DE SU EXPOSICION A LA SOLUCION DE VITRIFICACION. LA COMBINACION DE LIOFILIZACION Y VITRIFICACION ELIMINA APROXIMADAMENTE DESDE UN 80 % A UN 95 % DEL AGUA DE LAS CELULAS VEGETALES. LAS CELULAS PUEDEN SER CRIOCONSERVADAS CON EXITO DURANTE LARGOS PERIODOS DE TIEMPO Y RECUPERADAS DE FORMA VIABLE. LA INVENCION TAMBIEN TRATA DE PROCEDIMIENTOS PARA LA RECUPERACION DE CELULAS VEGETALES VIABLES PROCEDENTES DE LA CRIOCONSERVACION. LAS CELULAS SE DESCONGELAN HASTA APROXIMADAMENTE LA TEMPERATURA AMBIENTE Y SE INCUBAN EN UN MEDIO QUE CONTIENE UN AGENTE CRIOPROTECTOR Y UN ESTABILIZANTE. EL AGENTE CRIOPROTECTOR SE RETIRA Y LAS CELULAS SE INCUBAN Y SE RECUPERAN CON EXITO EN UN MEDIO DE CRECIMIENTO LIQUIDO O SEMI SOLIDO. LA INVENCION TAMBIEN TRATA DE LAS CELULAS CRIOCONSERVADAS Y DE CELULAS VEGETALES VIABLES QUE HAN SIDO RECUPERADAS PROCEDENTES DE UNA CRIOCONSERVACION A CORTO O LARGO PLAZO.
ES96923280T 1995-06-07 1996-06-07 Crioconservacion de diversas celulas vegetales. Expired - Lifetime ES2187664T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/486,204 US5965438A (en) 1995-06-07 1995-06-07 Cryopreservation of plant cells

Publications (1)

Publication Number Publication Date
ES2187664T3 true ES2187664T3 (es) 2003-06-16

Family

ID=23931007

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96923280T Expired - Lifetime ES2187664T3 (es) 1995-06-07 1996-06-07 Crioconservacion de diversas celulas vegetales.

Country Status (14)

Country Link
US (4) US5965438A (es)
EP (1) EP0830059B1 (es)
JP (2) JPH11507380A (es)
KR (1) KR100763858B1 (es)
AT (1) ATE227071T1 (es)
AU (1) AU701381B2 (es)
CA (1) CA2223959A1 (es)
DE (1) DE69624701T2 (es)
DK (1) DK0830059T3 (es)
ES (1) ES2187664T3 (es)
GB (1) GB2316854B (es)
NZ (1) NZ312329A (es)
PT (1) PT830059E (es)
WO (1) WO1996039812A2 (es)

Families Citing this family (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7264951B1 (en) 1992-02-20 2007-09-04 Phyton, Inc. Enhanced production of taxol and taxanes by cell cultures of Taxus species
US5965438A (en) * 1995-06-07 1999-10-12 Phyton, Inc. Cryopreservation of plant cells
US20050158699A1 (en) * 1995-06-07 2005-07-21 Kadkade Prakash G. Cryopreservation of plant cells
EP1538214A1 (en) 1996-05-24 2005-06-08 Phyton, Inc. Enhanced production of taxanes by cell cultures of taxus species
NL1009437C2 (nl) * 1998-06-18 1999-12-21 Xenobiosis Extractiewerkwijze.
US6176089B1 (en) * 1998-10-27 2001-01-23 Modex Th{acute over (e)}rapeutics Methods and compositions for cryopreservation of cells and tissues
US6280925B1 (en) * 1999-02-11 2001-08-28 Organ Recovery Systems, Inc. Polyethylene glycol and glutathione composition and method for the treatment of blood vessels prior to cryopreservation
US6682931B2 (en) 1999-05-25 2004-01-27 Meadwestvaco Corporation Recovering cryopreserved conifer embryogenic cultures
KR100390385B1 (ko) * 2000-07-03 2003-07-07 장덕진 미생물의 동결건조방법
US20030077566A1 (en) * 2001-09-10 2003-04-24 Palasz Andrzej T. Method for cryopreserving mammalian cells and tissues
US20030091659A1 (en) * 2001-11-09 2003-05-15 Avon Products, Inc. Topical composition having undifferentiated plant seed cells and method for using same
US20030124205A1 (en) * 2001-12-28 2003-07-03 Avon Products, Inc. Topical cosmetic composition having a natural plant active ingredient and method of using same
US8012753B2 (en) * 2003-07-30 2011-09-06 Weyerhaeuser Nr Company Methods and compositions for regrowth of cryopreserved conifer embryos
PL1720974T5 (pl) * 2004-02-24 2023-09-18 Chr. Hansen A/S Zamrożona kultura bakterii kwasu mlekowego w oddzielnych peletkach
KR100697317B1 (ko) * 2004-09-07 2007-03-20 바이오컬쳐(주) 봉선화의 원형을 장기보존하기 위한 동결건조방법
AR051477A1 (es) * 2004-11-05 2007-01-17 Dow Agrosciences Llc Crioconservacion de celulas
CA2512667A1 (en) * 2005-01-07 2006-07-07 Takahiro Ochiya Human hepatocyte-like cells and uses thereof
US7923249B2 (en) * 2005-03-10 2011-04-12 Cellfor Inc. Aerated liquid priming of conifer somatic germinants
US8192474B2 (en) 2006-09-26 2012-06-05 Zeltiq Aesthetics, Inc. Tissue treatment methods
US20080077201A1 (en) 2006-09-26 2008-03-27 Juniper Medical, Inc. Cooling devices with flexible sensors
US9132031B2 (en) 2006-09-26 2015-09-15 Zeltiq Aesthetics, Inc. Cooling device having a plurality of controllable cooling elements to provide a predetermined cooling profile
KR100868602B1 (ko) 2007-03-05 2008-11-13 김주태 인간 적혈구가 고농도 트리할로즈에 노출되었을 때발생하는 세포 용혈 현상을 최소화하는 방법
WO2008108549A1 (en) * 2007-03-05 2008-09-12 Jootae Kim Method on long-term structural preservation of hemocyte utilizing cellular lyophilization technique
EP3290520B1 (en) * 2007-03-09 2021-07-14 Monsanto Technology LLC Preparation and use of plant embryo explants for transformation
KR100845877B1 (ko) * 2007-04-05 2008-07-14 인하대학교 산학협력단 형질전환 벼세포의 동결보존 방법
DE102007025277A1 (de) * 2007-05-31 2008-12-04 Qiagen Gmbh Verfahren zur Stabilisierung einer biologischen Probe
US7785885B2 (en) * 2007-07-18 2010-08-31 Dow Agrosciences Llc Rescue of plant cell cultures and suspensions after cryopreservation-induced damage
WO2009026471A1 (en) 2007-08-21 2009-02-26 Zeltiq Aesthetics, Inc. Monitoring the cooling of subcutaneous lipid-rich cells, such as the cooling of adipose tissue
KR101064518B1 (ko) * 2007-09-21 2011-09-19 주식회사 운화 저장근을 가지는 초본식물의 형성층 유래 식물줄기세포주 및 이의 분리방법
EP2201084B1 (en) * 2007-10-04 2013-03-27 Petr Dejmek Method for the conservation of a plant material
WO2011005502A2 (en) 2009-06-23 2011-01-13 Biolex Therapeutics, Inc. Methods and compositions for the cryopreservation of duckweed
KR101185827B1 (ko) 2009-11-10 2012-09-27 대한민국 삼나무종자의 초저온 저장방법
EP2552478B1 (en) * 2010-03-31 2016-12-21 Stabilitech Ltd. Excipients for stabilising viral particles
JP5960120B2 (ja) 2010-03-31 2016-08-02 スタビリテック リミテッド ウイルス粒子の安定化
GB201012090D0 (en) * 2010-07-19 2010-09-01 British American Tobacco Co Cellulosic material
GB201117233D0 (en) 2011-10-05 2011-11-16 Stabilitech Ltd Stabilisation of polypeptides
CN102499083B (zh) * 2011-10-25 2013-06-12 广西壮族自治区药用植物园 一种广豆根组培苗叶柄的生根方法
JP6148032B2 (ja) * 2013-02-20 2017-06-14 大陽日酸株式会社 予備凍結装置
CN103283716B (zh) * 2013-06-08 2014-11-26 中南民族大学 一种简便的植物by-2细胞冷冻保存方法
WO2015064380A1 (ja) 2013-10-29 2015-05-07 学校法人北里研究所 細胞または組織のガラス化凍結保存用治具
WO2015117026A2 (en) 2014-01-31 2015-08-06 Zeltiq Aesthetics, Inc. Treating systems and methods for treating cellulite and providing other treatments
GB201406569D0 (en) 2014-04-11 2014-05-28 Stabilitech Ltd Vaccine compositions
CN103975852B (zh) * 2014-04-16 2016-05-18 西北大学 一种新疆雪莲愈伤组织超低温保存的方法
JP6124845B2 (ja) 2014-06-30 2017-05-10 三菱製紙株式会社 細胞又は組織のガラス化凍結保存用治具
CN104126570B (zh) * 2014-07-31 2016-05-18 甘肃中医学院 一种当归细胞超低温保存及植株再生的方法
CN104221882B (zh) * 2014-10-17 2017-01-18 山东农业大学 银杏组织培养外植体消毒方法
CN104285801A (zh) * 2014-10-17 2015-01-21 山东农业大学 银杏观赏品种定芽增殖方法
CN106795475B (zh) 2014-10-23 2021-01-29 三菱制纸株式会社 细胞或组织的冷冻保存用夹具及冷冻保存方法
CN104336009B (zh) * 2014-11-10 2016-03-30 中国科学院昆明植物研究所 富民枳微滴玻璃化法超低温保存方法
US11154418B2 (en) 2015-10-19 2021-10-26 Zeltiq Aesthetics, Inc. Vascular treatment systems, cooling devices, and methods for cooling vascular structures
WO2017099865A1 (en) 2015-12-07 2017-06-15 Coopersurgical, Inc. Low temperature specimen carriers and related methods
US10765552B2 (en) 2016-02-18 2020-09-08 Zeltiq Aesthetics, Inc. Cooling cup applicators with contoured heads and liner assemblies
CN105766894B (zh) * 2016-05-18 2018-10-19 中国农业科学院特产研究所 人参丛生芽超低温保存及植株再生培养方法
CN106417033B (zh) * 2016-11-10 2018-09-04 聊城大学 一种高唐栝楼快速繁殖方法
CN108077241B (zh) * 2016-11-22 2021-01-01 华东理工大学 活细胞传感仪在植物细胞低温保藏中的应用
CN106613838B (zh) * 2016-11-29 2019-09-13 中国科学院昆明植物研究所 一种提高拟南芥茎尖超低温保存后再生率的方法
US11076879B2 (en) 2017-04-26 2021-08-03 Zeltiq Aesthetics, Inc. Shallow surface cryotherapy applicators and related technology
JP6300215B1 (ja) * 2017-04-27 2018-03-28 節三 田中 植物の特性を増強する方法
CA3061616A1 (en) * 2017-04-27 2019-10-25 Setsuzo TANAKA Method for enhancing plant characteristics and method for producing seedless fruit
GB2562241B (en) 2017-05-08 2022-04-06 Stabilitech Biopharma Ltd Vaccine compositions
KR102288612B1 (ko) 2017-06-12 2021-08-11 미쓰비시 세이시 가부시키가이샤 세포 또는 조직의 동결 보존용 지그
JP6716140B6 (ja) * 2017-10-10 2020-07-29 節三 田中 植物の特性を増強する方法
JP6945487B2 (ja) 2018-04-26 2021-10-06 三菱製紙株式会社 細胞又は組織の凍結保存液
EP3801569A4 (en) * 2018-05-31 2022-03-30 Vitalant METHODS AND SYSTEMS FOR CRYOCONSERVATION AND RESUSPENDING ORGANIC LIQUIDS
CN108633741A (zh) * 2018-07-16 2018-10-12 河南农业大学 四季秋海棠的组织培养方法
CA3107932A1 (en) 2018-07-31 2020-02-06 Zeltiq Aesthetics, Inc. Methods, devices, and systems for improving skin characteristics
CN109207419A (zh) * 2018-08-22 2019-01-15 广西大学 甘蔗原生质体超低温保存方法
CN109221094A (zh) * 2018-09-11 2019-01-18 中国农业科学院作物科学研究所 一种香蕉离体茎尖超低温保存方法
CN110050784A (zh) * 2019-05-23 2019-07-26 南京林业大学 抗松材线虫病马尾松胚性愈伤组织超低温保存方法
WO2020242970A1 (en) * 2019-05-24 2020-12-03 East Carolina University Preservation using sugars
WO2020250766A1 (ja) 2019-06-12 2020-12-17 三菱製紙株式会社 凍結保存用治具の固定具および該固定具を用いた凍結融解方法
EA036602B1 (ru) * 2019-06-13 2020-11-27 Федеральное Государственное Бюджетное Учреждение Науки Институт Физиологии Растений Им. К.А. Тимирязева Российской Академии Наук Способ криосохранения меристематических верхушек побегов, изолированных из растений in vitro
JP7144380B2 (ja) 2019-08-23 2022-09-29 三菱製紙株式会社 細胞又は組織の凍結保存用治具
WO2021046200A1 (en) * 2019-09-06 2021-03-11 Cylerus, Inc. Stable long-term infusable formulations and methods
CN111149795A (zh) * 2020-01-14 2020-05-15 成都艾伟孚生物科技有限公司 一种冷冻保护剂及其应用和一种精子冷冻液及其制备方法
CN112690273A (zh) * 2020-04-14 2021-04-23 中国林业科学研究院 欧洲云杉胚性愈伤组织超低温保存的方法
CN111387176B (zh) * 2020-04-24 2021-07-30 中国科学院昆明植物研究所 一种厚朴胚性愈伤组织玻璃化超低温保存方法
CN113693060A (zh) * 2020-05-21 2021-11-26 中国农业科学院作物科学研究所 一种芋茎尖超低温保存方法
CN111771876A (zh) * 2020-08-18 2020-10-16 中国科学院合肥物质科学研究院 一种滁菊茎尖玻璃化超低温保存的方法
CN112056386B (zh) * 2020-09-03 2022-10-28 余姚谷满仓食品有限公司 一种速冻绿花菜的加工工艺
KR102487654B1 (ko) 2020-12-02 2023-01-12 대한민국 돼지 난자의 유리화 동결 보존용 도구 및 이를 이용한 돼지 난자의 유리화 동결 보존방법
CN113243359B (zh) * 2021-05-07 2022-09-27 山东省农业科学院 一种食蚜瘿蚊冷藏保存剂及保存方法
CN113349227B (zh) * 2021-05-12 2022-06-17 湖南省烟草公司株洲市公司 一种烟草种子促发芽抗逆壮苗组合物、制剂及使用方法
CN113841614A (zh) * 2021-11-01 2021-12-28 北京清美科技集团有限公司 一种植物超低温脱毒方法
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WO2023211926A2 (en) * 2022-04-25 2023-11-02 The Regents Of The University Of Colorado A Body Corporate System and methods to measure cell viability in high throughput via continuous geometry
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Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4191555A (en) * 1978-07-14 1980-03-04 Gaf Corporation Monaza amides and amines as ethylene inhibitors
HU190214B (en) * 1983-12-30 1986-08-28 Richter Gedeon Vegyeszeti Gyar Rt,Hu Process for the promotion of regenerating plants from tissue culture
US5071741A (en) * 1988-04-18 1991-12-10 Cryolife, Inc. Cryoprotective agent and its use in cryopreservation of cellular matter
US5045446A (en) * 1988-08-26 1991-09-03 Cryopharm Corporation Lyophilization of cells
US5041382A (en) * 1989-03-09 1991-08-20 Weyerhaeuser Company High concentration enrichment of conifer embryonal cells
NZ246137A (en) * 1991-12-19 1996-04-26 Univ Saskatchewan Mature desiccation tolerant gymnosperm somatic embryos and their production
JPH07507443A (ja) * 1992-01-21 1995-08-24 コーベ　ラボラトリーズ　インコーポレイテッド 細胞および細胞様物質を凍結する方法
CA2092849C (en) * 1992-04-07 2003-11-18 Paul M. Cino Callus cell induction and the preparation of taxanes
AU4538293A (en) * 1992-06-16 1994-01-04 Reagents Of The University Of California, The Method for identifying plant pathogen tolerance
KR970009157B1 (en) * 1993-06-12 1997-06-07 Green Cross Corp Process for the high yield production of taxol by cell culture
US5506136A (en) * 1993-10-21 1996-04-09 Westvaco Corporation Method for regeneration of coniferous plants by somatic embryogenesis
US5413930A (en) * 1993-10-21 1995-05-09 Westvaco Corporation Method for regeneration of coniferous plants by somatic embryogenesis
US5491090A (en) * 1994-02-09 1996-02-13 Westvaco Corporation Embryogenic coniferous liquid suspension cultures
US5736369A (en) * 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
US5811274A (en) * 1994-12-09 1998-09-22 The Regents Of The University Of Michigan Methods, compositions and apparatus for cell transfection
WO1996036693A1 (en) 1995-05-19 1996-11-21 Phytera, Inc. Manipulation of plant cell and tissue cultures
US5965438A (en) * 1995-06-07 1999-10-12 Phyton, Inc. Cryopreservation of plant cells

Also Published As

Publication number Publication date
PT830059E (pt) 2003-03-31
JP2008005846A (ja) 2008-01-17
ATE227071T1 (de) 2002-11-15
EP0830059B1 (en) 2002-11-06
NZ312329A (en) 1999-09-29
AU701381B2 (en) 1999-01-28
DK0830059T3 (da) 2003-03-03
WO1996039812A2 (en) 1996-12-19
US20030031998A1 (en) 2003-02-13
GB2316854B (en) 1998-11-11
DE69624701D1 (de) 2002-12-12
GB2316854A (en) 1998-03-11
US6753182B1 (en) 2004-06-22
KR19990022412A (ko) 1999-03-25
JPH11507380A (ja) 1999-06-29
CA2223959A1 (en) 1996-12-19
EP0830059A2 (en) 1998-03-25
AU6383696A (en) 1996-12-30
US5965438A (en) 1999-10-12
GB9725211D0 (en) 1998-01-28
DE69624701T2 (de) 2003-10-16
US6127181A (en) 2000-10-03
KR100763858B1 (ko) 2008-11-07
WO1996039812A3 (en) 1997-05-29

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