EP1157743A1 - Mikrofabriziertes poröses Durchflussgerät zur diskreten Bestimmung von Bindungsreaktionen - Google Patents

Mikrofabriziertes poröses Durchflussgerät zur diskreten Bestimmung von Bindungsreaktionen Download PDF

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EP1157743A1
EP1157743A1 EP01118897A EP01118897A EP1157743A1 EP 1157743 A1 EP1157743 A1 EP 1157743A1 EP 01118897 A EP01118897 A EP 01118897A EP 01118897 A EP01118897 A EP 01118897A EP 1157743 A1 EP1157743 A1 EP 1157743A1
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wafer
binding
regions
substrate
hybridization
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EP1157743B1 (de
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Kenneth L. Beattie
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Houston Advanced Research Center HARC
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Definitions

  • the term "genosensor” has heretofore referred to a form of SBH in which oligonucleotides are tethered to a surface in a two-dimensional array and serve as recognition elements for complementary sequences present in a nucleic acid "target” sequence.
  • the genosensor concept further includes microfabricated devices in which microelectronic components are present in each test site, permitting rapid, addressable detection of hybridization across the array.
  • the present invention provides a novel flow-through genosensor, in which nucleic acid recognition elements are immobilized within densely packed pores or channels, arranged in patches across a wafer of solid support material.
  • Known microfabrication techniques are available for producing microchannel or nanochannel glass and porous silicon useful as support wafers.
  • Flow-through genosensors utilize a variety of conventional detection methods, including microfabricated optical and electronic detection components, film, charge-coupled-device arrays, camera systems and phosphor storage technology.
  • the present invention provides an improved apparatus and method
  • the present invention specifically provides novel high-density and ultra-high density microfabricated, porous devices for the conduction and detection of binding reactions.
  • the present invention provides improved "genosenors" and methods for the use thereof in the identification or characterization of nucleic acid sequences through nucleic acid probe hybridization with samples containing an uncharacterized polynucleic acid, e.g., a cDNA, mRNA, recombinant DNA, polymerase chain reaction (PRC) fragments or the like, as well as other biomolecules.
  • an uncharacterized polynucleic acid e.g., a cDNA, mRNA, recombinant DNA, polymerase chain reaction (PRC) fragments or the like, as well as other biomolecules.
  • PRC polymerase chain reaction
  • microfabrication technology has revolutionized the electronics industry and has enabled miniaturization and automation of manufacturing processes in numerous industries.
  • the impact of microfabrication technology in biomedical research can be seen in the growing presence of microprocessor-controlled analytical instrumentation and robotics in the laboratory, which is particularly evident in laboratories engaged in high throughput genome mapping and sequencing.
  • the Human Genome Project is a prime example of a task that whose economics would greatly benefit from microfabricated high-density and ultra-high density hybridization devices that can be broadly applied in genome mapping and sequencing.
  • Hybridization of membrane-immobilized DNAs with labeled DNA probes is a widely used analytical procedure in genome mapping.
  • Robotic devices currently enable gridding of 10,000-15,000 different target DNAs onto a 12 cm x 8 cm membrane.
  • Hybridization of DNA probes to such filters has numerous applications in genome mapping, including generation of linearly ordered libraries, mapping of cloned genomic segments to specific chromosomes or megaYACs, cross connection of cloned sequences in cDNA and genomic libraries, etc.
  • Recent initiatives in "sequencing by hybridization” (SBH) aim toward miniaturized, high density hybridization arrays.
  • SBH sequencing by hybridization
  • a serious limitation to miniaturization of hybridization arrays in membranes or on flat surfaces is the quantity of DNA present per unit cross sectional area, which (on a two-dimensional surface) is a function of the surface area. This parameter governs the yield of hybridized DNA and thus the detection sensitivity.
  • Genosensors, or miniaturized "DNA chips” are currently being developed in several laboratories for hybridization analysis of DNA samples.
  • DNA chips typically employ arrays of DNA probes tethered to flat surfaces, e.g., Fodor, S.P.A., Read, J.L., Pirrung, M.C., Stryer, L., Lu, A.T. and Solas, D. 1991. Science 251:767-773, Southern, E.M., Maskos, U. and Elder, J.K. 1992.
  • Genomics 13:1008-1017 Eggers, M.D., Hogan, M.E., Reigh, R.K., Lamture, J.B., Beattie, K.L., Hollis, M.A., Ehrlich, D.J., Kosicki, B.B., Shumaker, J.M., Varma, R.S., Burke, B.E., Murphy, A, and Rathman, D.D. 1993. Advances in DNA Sequencing Technology, SPIE Conference, Los Angeles, CA, and Beattie, K., Eggers, M., Shumaker, J., Hogan, M., Varma, R., Lamture, J., Hollis, M., Ehrlich, D. and Rathman, D. 1993.
  • microfabricated apparatus is designed to overcome the inherent limitations in current solid phase hybridization materials, eliminating the diffusion-limited step in flat surface hybridizations and increasing the cross sectional density of DNA.
  • microfabricated genosensor devices are characterized by a compact physical size and the density of components located therein.
  • Known microfabricated binding devices are typically rectangular wafer-type apparatuses with a surface area of approximate one cm 2 , e.g., 1 cm x 1 cm.
  • the bounded regions on such devices are typically present in a density of 10 2 -10 4 regions/cm 2 , although the desirability of constructing apparatuses with much higher densities has been regarded as an important objective. See Eggers and Beattie, cited above, for discussion of strategies for the construction of devices with higher densities for the bounded regions.
  • microfabricated apparatuses as described herein are known to be useful for a variety of analytical tasks, including nucleic acid sequence analysis by hybridization (SBH), analysis of patterns of gene expression by hybridization of cellular mRNA to an array of gene-specific probes, immunochemical analysis of protein mixtures, epitope mapping, assay of receptor-ligand interactions, and profiling of cellular populations involving binding of cell surface molecules to specific ligands or receptors immobilized within individual binding sites.
  • SBH nucleic acid sequence analysis by hybridization
  • analysis of patterns of gene expression by hybridization of cellular mRNA to an array of gene-specific probes immunochemical analysis of protein mixtures
  • epitope mapping assay of receptor-ligand interactions
  • profiling of cellular populations involving binding of cell surface molecules to specific ligands or receptors immobilized within individual binding sites profiling of cellular populations involving binding of cell surface molecules to specific ligands or receptors immobilized within individual binding sites.
  • nucleic acid analysis is one principal use for such an microapparatus,
  • the microfabricated apparatus is used in conjunction with a known detection technology particularly adapted to discriminating between bounded regions in which binding has taken place and those in which no binding has occurred and for quantitating the relative extent of binding in different bounded regions.
  • autoradiography and optical detection are advantageously used. Autoradiography is performed using 32 P or 35 S labelled samples.
  • nucleic acid fragments are end-labeled with 32 P and these end-labeled fragments are separated by size and then placed adjacent to x-ray film as needed to expose the film, a function of the amount of radioactivity adjacent to a region of film.
  • phophorimager detection methods may be used.
  • Optical detection of fluorescent-labelled receptors is also employed in detection.
  • a DNA base-specific fluorescent dye is attached covalently to the oligonucleotide primers or to the chain-terminating dideoxynucleotides used in conjunction with DNA polymerase.
  • the appropriate absorption wavelength for each dye is chosen and used to excite the dye. If the absorption spectra of the dyes are close to each other, a specific wavelength can be chosen to excite the entire set of dyes.
  • One particularly useful optical detection technique involves the use of ethidium bromide, which stains duplex nucleic acids.
  • the fluorescence of these dyes exhibits an approximate twenty-fold increase when it is bound to duplexed DNA or RNA, when compared to the fluorescence exhibited by unbound dye or dye bound to single-stranded DNA.
  • This dye is advantageously used to detect the presence of hybridized polynucleic acids.
  • a highly preferred method of detection is a charge-coupled-device array or CCD array.
  • CCD array With the CCD array, a individual pixel or group of pixels within the CCD array is placed adjacent to each confined region of the substrate where detection is to be undertaken. Light attenuation, caused by the greater absorption of an illuminating light in test sites with hybridized molecules, is used to determine the sites where hybridization has taken place. Lens-based CCD cameras can also be used.
  • a detection apparatus can be constructed such that sensing of changes in AC conductance or the dissipation of a capacitor placed contiguous to each confined region can be measured.
  • RF radio-frequence
  • Methods for attaching samples of substantially homogeneous biomolecules of a pre-determined structure to the confined regions of the microapparatus are likewise known.
  • One preferred method of doing so is to attach these biomolecules covalently to surfaces such as glass or gold films.
  • methods for attachments of oligonucleotide probes to glass surfaces are known.
  • a primary amine is introduced at one terminus during the chemical synthesis thereof.
  • one or more triethylene glycol units may be introduced therebetween as spacer units.
  • the primary amine terminus of the oligonucleotide can be covalently attached thereto.
  • Khrapko, K.R., et al. A method for DNA sequencing by hybridization with oligonucleotide matrix , J. DNA Sequencing and Mapping, 1 :375-388 (1991), Drmanac, Radoje, et al., Sequencing by hybridization: Towards an automated sequencing of one million M13 clones arrayed on membranes Electrophoresis 13:566-573 (1992), Meier-Ewert, Sebastian, An automated approach to generating expressed sequence catalogues, Nature 361 :375-376 (1993), Drmanac, R., et al., DNA Sequence Determination by Hybridization: A Strategy for Efficient Large-Scale Sequencing, Science 260 :1649-1652 (1993), Southern, E.M., et al., Analyzing and Comparing Nucleic Acid Sequences by Hybridization to Arrays of Oligonucleotides: Evaluation Using Experimental Models, Genomics 13 :1008-1017 (19
  • the present invention particularly provides:
  • the devices of the present invention are used to characterize or otherwise identify molecular species capable of controllably binding to biomolecules in the same manner and using the same binding regimens as are known in the art. Although uses of these novel devices include antibody-antigen and ligand-receptor binding, a major use of the present invention is in the field of nucleic acid sequence analysis. Two fundamental properties of DNA are vital to its coding and replicational functions in the cell:
  • the specific pattern of base pairing is vital to the proper functioning of nucleic acids in cells, and also comprises a highly specific means for the analysis of nucleic acid sequences outside the cell.
  • a nucleic acid strand of specific base sequence can be used as a sequence recognition element to "probe" for the presence of the perfectly “complementary” sequence within a nucleic acid sample (Conner, et al., Proc. Natl . Acad . Sci ., U.S.A ., 80:278-282 (1983)).
  • a sample of DNA or RNA is "annealed” or “hybridized” with a nucleic acid "probe” containing a specific base sequence
  • the probe will bind to the nucleic acid "target” strand only if there is perfect (or near-perfect) sequence complementarity between probe and target.
  • the hybridization event which indicates the presence of a specific base sequence in a nucleic acid sample is typically detected by immobilization of the nucleic acid sample or the probe on a surface, followed by capture of a "tag” (for example, radioactivity or fluorescence) carried by the complementary sequence.
  • DNA hybridization has been employed to probe for sequence identity or difference between DNA samples, for example in the detection of mutations within specific genetic regions (Kidd, et al., N. Engl. J. Med., 310:639-642 (1984); Saiki, et al., N. Engl. J. Med., 319:537-541 (1988); Saiki, et al., Proc. Natl. Acad. Sci., U.S.A., 86:6230-6234 (1989)).
  • DNA probe analysis is a useful means for detection of mutations associated with genetic diseases, the current methods are limited by the necessity of performing a separate hybridization reaction for detection of each mutation.
  • DNA sequence polymorphisms are DNA sequence changes at any given genetic locus which are maintained in a significant fraction of the individuals within a population. DNA sequence polymorphisms can serve as useful markers in genetic mapping when the detectable DNA sequence changes are closely linked to phenotypic markers and occur at a frequency of at least 5% of the individuals within a population. In addition, polymorphisms are employed in forensic identification and paternity testing. Currently employed methods for detecting genetic polymorphisms involve laborious searches for "restriction fragment length polymorphisms" (RFLPS) (Lander and Bottstein, Proc, Natl.
  • DNA sequence variation is that which occurs between species of organisms, which is of significance for several reasons.
  • identification of sequence differences between species can assist in the determination of the molecular basis of phenotypic differences between species.
  • Second, a survey of sequence variation within a specific gene among numerous related species can elucidate a spectrum of allowable amino acid substitutions within the protein product encoded by the gene, and this information is valuable in the determination of structure-function relationships and in protein engineering programs.
  • this type of targeted DNA sequence comparison is extremely laborious, time consuming and costly if carried out by current DNA sequencing methodology.
  • genetic sequence variation can form the basis of specific identification of organisms, for example, infectious micro-organisms.
  • the apparatus of the present invention is employed in a variety of analytical tasks, including nucleic acid sequence analysis by hybridization, analysis of patterns of gene expression by hybridization of cellular mRNA to an array of gene-specific probes, immunochemical analysis of protein mixtures, epitope mapping, assay of receptor-ligand interactions, and profiling of cellular populations involving binding of cell surface molecules to specific ligands or receptors immobilized within individual binding sites.
  • nucleic acid analysis is specifically taught in this disclosure, the present invention can be equally applied to a broad range of molecular binding reactions involving small molecules, macromolecules, particles, and cellular systems.
  • NCG materials are unique glass structures containing a regular geometric array of parallel holes or channels as small as 33 nm in diameter or as large as several micrometers in diameter. See Tonucci, R.J., Justus, B.L., Campillo, A.J. and Ford, C.E. 1992. Science 258:783-785. These nanochannel glass structures can possess packing densities in excess of 3x10 10 channels per square centimeter, fabricated in various array configurations. A variety of materials can be immobolized or fixed to the glass surfaces within the channels of the NCG array, to yield a high surface area to volume ratio.
  • Nanochannel glass arrays are fabricated by arranging dissimilar glasses in a predetermined configuration, where at least one glass type is usually acid etchable. Construction of a two-dimensional hexagonal close packing array begins by insertion of a cylindrical acid etchable glass rod (referred to as the channel glass) into an inert glass tube (referred to as the matrix glass) whose inner dimensions match that of the rod. The pair is then drawn under vacuum to reduce the overall cross-section to that of a fine filament. The filaments are then stacked, re-fused and redrawn. This process is continued until appropriate channel diameters and the desired number of array elements are achieved. By adjusting the ratio of the diameter of the etchable glass rod to that of the outside dimension of the inert glass tubing, the center-to-center spacing of the rods and their diameters in the finished product become independently adjustable parameters.
  • the NCG material is wafered perpendicular to the direction of the channels with a diamond saw and then polished to produce 0.1-1.0 mm sections of material.
  • the channel glass of the array structure is then etched away with an acid solution.
  • a hexagonal close packing arrangement of channel glasses, after acid etching, contains typically 10 7 channels and is uniform throughout.
  • the channel diameter is typically 450 nm and the center-to-center spacing is approximately 750 nm.
  • the type of array structure described above is useful in the NCG array hybridization assembly in accordance with the present invention.
  • the tapered sample well structure defines each group of channels serving as a specific hybridization test site.
  • a second type of hexagonal array structure in which separated clusters of channels are formed during the fabrication process, exhibits an open array structure with typical channel diameters of 300nm.
  • the overall glass structure consists of an array of 18 ⁇ m diameter subarrays, each serving to contain a specific DNA probe or target, and spaced typically 25 ⁇ m apart from neighboring arrays.
  • NCG hybridization arrays described in Example 1 are bonded on the upper side to a polymeric layer containing an array of orifices which align with the array of nanochannel bundles and serve as sample wells for placement of a substantially homogeneous sample of a biomolecule (e.g., a single DNA species) within each hybridization site.
  • This polymeric sample well array also provides physical support to the fragile NCG wafer.
  • the polymeric array of orifices are fabricated using methods known in the art.
  • this polymeric layer suitable for use herein can be obtained from MicroFab Technologies, Inc.
  • the orifices are fabricated using excimer laser machining. This method is preferred because existing technology is employed allowing for low cost/high volume manufacturing, as is currently being done in the microelectronics industry.
  • Development of the polymeric array comprises four task: (1) materials selection; (2) ablation tooling and process development; (3) lamination tooling and process development; and (4) production of high density and ultra-high density polymeric arrays. These tasks are undertaken as follows:
  • Part A Materials selection.
  • the materials useful in the polymeric array are filled polymers, epoxy resins and related composite (e.g., "circuit-board”-type) materials. Because it is a standard process in the microelectronics industry, the present invention most advantageously employs polymeric materials with the adhesive applied by the commercial vendor of the material, for example, a polyamide with a 12 ⁇ m thick layer of a B-stage (heat curing) adhesive
  • the primary requirements for the polymeric array material to be used are:
  • Part B Ablation tooling and processing
  • Contact mask excimer laser machining is a preferred processing technique because it is a lower cost technique than projection mask excimer laser machining.
  • a projection mask is, however, employed when the feature size less than 50 ⁇ m.
  • One or more masks with a variety of pattern sizes and shapes are fabricated, along with fixtures to hold the mask and material to be ablated. These masks are employed to determine the optimal material for laser machining and the optimal machining conditions (i.e., mask hole size, energy density, input rate, etc.). Scanning electron microscopy and optical microscopy are used to inspect the excimer laser machined parts, and to quantify the dimensions obtained, including the variation in the dimensions.
  • the adhesive material is also ablated. This second ablation is undertaken so that the diameter of the hole in the adhesive is made larger than diameter of the sample well on the adhesive side of the polymeric material. This prevents the adhesive from spreading into the sample well and/or the nanoporous glass during lamination.
  • Part C Lamination tooling and processing
  • Initial lamination process development is carried out using unablated polymeric material (or alternatively, using glass slides and/or silicon wafers). Cure temperature, pressure, and fixturing are optimized during this process development. Thereafter, the optimized processing parameters are employed to laminate both nanoporous wafers and polymeric arrays. The final lamination is done such that the alignment of the two layers creates functional wells.
  • Part D Production of polymeric arrays
  • the optimal mask patterns and excimer laser parameters are determined and thereafter employed in the manufacture of contact masks and material holding fixtures. Typically, fabrication is done so as to produce a large number (>100) of parts as the masks wear out with use).
  • porous silicon devices Two general types are prepared according to the process described herein. First, known microfabrication methods are used to fabricate wafers, bounded by integral sample wells. Second, uniformily porous wafer structures are bonded to the same orifice sample well arrays that were described previously (Example 2) for NCG glass arrays. Porous silicon designs are advantageously employed herein because of their adaptability to low cost mass production processes and their ability to incorporate in the fabrication process structural elements that function in fluidic entry and exit from the hybridization site and structures (e.g., electrodes) that may function in hybridization detection.
  • Stable, open-cell porous materials are used to accomplish enhancements and to introduce qualitatively new features in these devices, whereby the surface area of discrete and isolated binding regions is increased by a factor of 100 to 1000 in hybridization-based electronic, fluorescence and radiation-based DNA detectors.
  • controlled introduction of high-surface-area supports at the surface detection site is employed.
  • Thin-film processing technology is used to deposit chemically inert and thermally stable microporous materials. Materials and processing methods are selected to achieve low-cost semiconductor batch fabrication of integrated semiconductor detectors.
  • the microchip device provides in situ multisite analysis of binding strength as ambient conditions are varied. Porous silicon materials are fabricated in oriented, pore arrays or random interconnected networks and with pore diameters selected over the range from 2 nm to several micrometers.
  • Porous silicon is produced most easily through electrochemical etching. It can be processed into two important pore structures, interconnected networks and oriented arrays. The pore diameter is tailored from approximately 2 nm to micrometer dimensions by selection of doping and electrochemical conditions. For n-type material, etching is thought to proceed through a tunneling mechanism in which electrons are injected into the pore surface through field concentration effects. In the case of p-type material the mechanism seems to be through moderation of carrier supply at the electrolyte/silicon interface. In practice, the following structures can be fabricated:
  • Characterization can be undertaken by scanning electron microscopy.
  • the surface wetting properties are varied using vapor treatment with silylation materials and chlorocarbons.
  • High-porosity dielectrics which function as molecular sieves are produced by nuclear track etching. While nuclear track etching is used to produce these molecular sieves in a wide range of inorganic materials, it is most often used with dielectrics such as mica and sapphire.
  • nuclear track etching is used to produce these molecular sieves in a wide range of inorganic materials, it is most often used with dielectrics such as mica and sapphire.
  • a substrate is first bombarded with nuclear particles (typically several MeV alpha particles) to produce disturbances or "tracks" within the normal lattice structure of the material and then wet-etched to produce pores which follow the tracks caused by the nuclear particles. More specifically, Price et al . disclose that the exposure of a mica substrate to heavy, energetic charged particles will result in the formation of a plurality of substantially straight tracks in its lattice structure and that these tracks can be converted into pores by wet etching the substrate.
  • nuclear particles typically several MeV alpha
  • Pore sizes and overall porosity are variably controllable with pores typically 0.2 ⁇ m in diameter and densities on the order of 10 9 /cm 2 .
  • Particle track depths are energy dependent on the incident particle beam, but resulting pores can be extended through an entire 500- ⁇ m-thick substrate. Incorporation of these materials on the device structures shown above is readily accomplished.
  • the use of implantation-etched dielectrics as the sensor element has advantages versus the porous silicon approach since the material is hydrophilic.
  • a preferred device is the porous silicon array wafer with integral sample wells illustrated in Figure 3.
  • This may be constructed as follows: A four inch diameter, 100 ⁇ m thick wafer of crystalline silicon (n-type, doped with 10 15 P/cm 3 ) with axis oriented along ⁇ 100> direction is coated with photoresist and exposed to light through a mask to define a 50 x 50 array of 200 ⁇ m square areas having 200 ⁇ m space between them across the 2 cm x 2cm central area of the wafer.
  • 140(100) :2836-2843 (1993)) is then used to create patches of closely spaced pores of diameter 2-5 ⁇ m, oriented perpendicular to the wafer surface, within each square area defined in the photolithographic step.
  • a 300 ⁇ m thick wafer of silicon dioxide is coated with photoresist and exposed to light through the same mask used to define 200 ⁇ m square porous regions in the silicon wafer, and acid etching is conducted to create 200 ⁇ m square holes in the silicon dioxide wafer.
  • the silicon dioxide wafer is then aligned with and laminated to the porous silicon wafer using a standard wafer bonding process to form the integral structure shown in the figure.
  • each pore is oxidized to form a layer of silicon dioxide.
  • the epoxysilane-amine linkage procedure described in EXAMPLE 4 is then carried out to covalently attach amine-containing biopolymer species to the walls of the pores.
  • a stock solution of epoxysilane is freshly prepared with the following proportions: 4 ml 3-glycidoxypropyl-trimethoxysilane, 12 ml xylene, 0.5 ml N,N-diisopropylethylamine (Hunig's base). This solution is flowed into the pores of the wafer, then the wafer is soaked for 5 hours in the solution at 80°C, then flushed with tetrahydrofuran, dried at 80°C, and placed in a vacuum desiccator over Drierrite or stored in a desiccator under dry argon.
  • Oligonucleotide bearing 5'- or 3'-alkylamine (introduced during the chemical synthesis) is dissolved at 10 ⁇ M - 50 ⁇ M in water and flowed into the porous silica wafer. After reaction at 65°C overnight the surface is briefly flushed with water at 65°C, then with 10mM triethylamine to cap off the unreacted epoxy groups on the surface, then flushed again with water at 65°C and air dried.
  • amine-derivatized oligonucleotides can be attached to epoxysilane-derivatized glass in dilute (eg., 10mM - 50mM) KOH at 37°C for several hours, although a higher background of nonspecific binding of target sample DNA to the surface (independent of base pairing) may occur during hybridization reaction.
  • a Hamilton Microlab 2200 robotic fluid delivery system equipped with special low volume syringes and 8-position fluid heads, capable of delivering volumes of 10-100 nl at 500 ⁇ m xyz stepping and a few percent precision.
  • 40-nl samples of biomolecules e.g., DNA, olgionucleotides and the like
  • a piezoelectrically controlled substage custom fitted for the Microlab 2200 permits xy positioning down to submicron resolution.
  • custom fabricated needles are employed.
  • the eight-needle linear fluid head is operated in staggered repetitive steps to generate the desired close spacing across the wafer.
  • the system has a large stage area and rapid motion control, providing the capacity to produce hundreds of replicate hybridization wafers.
  • Part A Microfab microfluidic jets
  • a microjet system capable of delivering subnanoliter DNA solutions to the wafer surface is employed as follows: For placement of DNA into individual hybridization sites within ultra-high density wafers, with volumes of one nl (corresponding to a 130 ⁇ m sphere or 100 ⁇ m cube) commercially available dispensing equipment using ink-jet technology as the microdispensing method for fluid volume below is employed.
  • the droplets produced using ink-jet technology are highly reproducible and can be controlled so that a droplet may be placed on a specific location at a specific time according to digitally stored image data.
  • Typical droplet diameters for demand mode ink-jet devices are 30-100 ⁇ m, which translates to droplet volumes of 14-520 pl.
  • Droplet creation rates for demand mode ink-jet devices are typically 2000-5000 droplets per second.
  • both the resolution and throughput of demand mode ink-jet microdispensing are in the ranges required for the ultrahigh density hybridization wafer.
  • Part B Microdispensing System
  • the microdispensing system is modified from a MicroFab drop-on-demand ink-jet type device, hereafter called a MicroJet device such that this type of device can produce 50 ⁇ m diameter droplets at a rate of 2000 per second.
  • a MicroJet device such that this type of device can produce 50 ⁇ m diameter droplets at a rate of 2000 per second.
  • the operating principles of this type of device are known (D.B. Wallace, "A Method of Characteristics Model of a Drop-On-Demand Ink-Jet Device Using an Integral Drop Formation Method," ASME publication 89-WA/FE-4, December 1989) and used to effect the modification.
  • To increase throughput eight of these devices are integrated into a line array less than 1 inch (25mm) long. The eight devices are loaded with reagent simultaneously, dispense sequentially, and flush simultaneously.
  • a means is provided for flow of liquids through the wafer.
  • the hybridization wafer To enable flow of liquid through the hybridization wafer, it is packaged within a 2 mm x 4 mm polypropylene frame, which serves as an upper reservoir and structure for handling.
  • a polypropylene vacuum chamber with a Delrin o-ring around its upper edge to permit clamping of the wafer onto the vacuum manifold to form a seal is employed.
  • the vacuum assembly is illustrated in Fig. 4.
  • For control of fluid flow through the wafer a screw-drive device with feedback control is provided.
  • Oligonucleotides to be used in the present invention are synthesized by the phosphoramidite chemistry (Beaucage, S.L. and Caruthers, M.H. 1981. Tet. Lett. 22:1859-1862) using the segmented synthesis strategy that is capable of producing over a hundred oligonucleotides simultaneously (Beattie, K.L., Logsdon, N.J., Anderson, R.S., Espinosa-Lara, J.M., Maldonado-Rodriguez, R. and Frost, J.D. Ill. 1988. Biotechnol. Appl. Biochem. 10:510-521; Beattie, K.L. and Fowler, R.F. 1991. Nature 352:548-54926,27).
  • the oligonucleotides can be derivatized with the alkylamino function during the chemical synthesis, either at the 5'-end or the 3'-end.
  • This versatile approach (using epoxy silane) is inexpensive and provides a dense array of monolayers that can be readily coupled to terminally modified (amino- or thiol-derivatized) oligonucleotides.
  • the density of probe attachment is controlled over a wide range by mixing long chain amino alcohols with the amine-derivatized oligonucleotides during attachment to epoxysilanized glass.
  • This strategy essentially produces a monolayer of tethered DNA, interspersed with shorter chain alcohols, resulting in attachment of oligonucleotides down to 50 ⁇ apart on the surface.
  • Variable length spacers are optionally introduced onto the ends of the oligonucleotides, by incorporation of triethylene glycol phosphoryl units during the chemical synthesis.
  • variable linker arms are useful for determining how far from the surface oligonucleotide probes should be separated to be readily accessible for pairing with the target DNA strands.
  • Thiol chemistry adapted from the method of Whitesides and coworkers on the generation of monolayers on gold surfaces (Randall lee, T., Laibinis, P. E., Folkers, J. P. and Whitesides, G. M. (1991) Pure & Appl. Chem. 63: 821-828 and references cited therein.), is used for attachment of DNA to gold and platinum surfaces.
  • Dithiols e.g., 1, 10-decanedithiol
  • a terminal, reactive thiol moiety for reaction with bromoacetylated oligonucleotides.
  • the density of attachment of DNA to gold or platinium surfaces is controlled at the surface-activation stage, by use of defined mixtures of mono- and dithiols.
  • Part B Surface Immobilization of Recombinant Vector DNA , cDNA and PCR Fragments
  • hexylamine groups are first incorporated into the target DNA using polymerization (PCR or random priming) in the presence of 5-hexylamine-dUTP, then a bromoacetylation step is carried out to activate the DNA for attachment to thiolated metal surfaces.
  • PCR polymerization
  • bromoacetylation step is carried out to activate the DNA for attachment to thiolated metal surfaces.
  • routine experimentation is employed (varying the dTTP/5-hexylamine-dUTP ratio and the attachment time) to define conditions that give reproducible hybridization results.
  • the foregoing procedure (omitting the bromoacetylation step) can also serve as an alternative method for immobilization of target DNA to glass surfaces..
  • the end attachment places the probes 50-100 ⁇ apart on the surface, corresponding to up to 10 8 probes in a 50 ⁇ m x 50 ⁇ m area.
  • Approximately 10 10 -10 11 oligonucleotide probes can be tethered within a 50 ⁇ m cube of porous silicon in the nanofabricated wafer.
  • the density of bound oligonucleotides per cross sectional area is estimated by end-labeling prior to the attachment reaction, then quantitating the radioactivity using the phosphorimager.
  • Known quantities of labeled oligonucleotides dried onto the surface are used to calibrate the measurements of binding density.
  • the target DNA is prepared by the polymerase chain reaction, incorporating [ 32 P]nucleotides into the product during the amplification or by using gamma- 32 P[ATP] + polynucleotide kinase to 5'-label the amplification product. Unincorporated label is removed by Centricon filtration.
  • one of the PCR fragments is 5'-biotin-labeled to enable preparation of single strands by streptavidin affinity chromatography.
  • the target DNA is dissolved in hybridization buffer (50mM Tris-HCl, pH 8, 2mM EDTA, 3.3M tetramethylammonium chloride) at a concentration of at least 5nM (5 fmol/ ⁇ l) and specific activity of at least 5,000 cpm/fmol.
  • hybridization buffer 50mM Tris-HCl, pH 8, 2mM EDTA, 3.3M tetramethylammonium chloride
  • hybridization can be carried out in buffer containing 1M KCL or NaCI or 5.2M Betaine, in place of tetramethylammonium chloride.
  • the temperature of hybridization and washing can be varied over the range 5°C to 30°C for hybridization with short oligonucleotides. Higher temperatures may be desired for hybridization using longer probes.
  • Part A Phosphorimager and film detection
  • the detection and quantitation of hybridization intensities is carried out using methods that are widely available: phosphorimager and film.
  • the Biorad phosphorimager has a sample resolution of about 100 ⁇ m and is capable of registering both beta emission and light emission from chemiluminescent tags.
  • Reagent kits for chemiluminescence detection available from Millipore and New England Nuclear, which produce light of 477 and 428 nm, respectively, are advantageously used with the Biorad instrument.
  • Chemiluminescent tags are introduced into the target DNA samples (random-primed vector DNA or PCR fragments) using the procedures recommended by the supplier. Thereafter, the DNA is hybridized to the nanoporous wafers bearing oligonucleotide probes.
  • Radioactive tags 32 P and 33 P, incorporated by random priming and PCR reaction are also used in these experiments. Film exposure is used for comparison.
  • radioactive tags incorporated using polynucleotide kinase
  • optimal chemiluminescent tagging procedures for oligonucleotides are generally not available.
  • CCD genosensor devices are capable of maximum resolution and sensitivity and are used with chemiluminescent, fluorescent and radioactive tags (Lamture, J.L., Varma, R., Fowler, R., Smith, S., Hogan, M., Beattie, K.L., Eggers, M., Ehrlick, D., Hollis, M. and Kosicki, B. 1993. Nature, submitted).
  • EXAMPLE 10 Genosensor Experiment; Mutation Detection in Exon 7/8 Region of Hamster hprt Gene
  • the hprt gene is used extensively as a model system for studies of mutation.
  • the gene has been cloned and sequenced from several mammals.
  • a variety of mutations in this gene are known and were characterized by DNA sequencing, in the hamster (induced by chemicals and radiation in Chinese Hamster Ovary cell lines) and from humans (associated with Lesch Nyhan syndrome).
  • a significant fraction of hprt mutations are found in a short region of the gene encoded by exons 7 and 8.
  • the nucleotide sequence of the normal and mutant genes are found in the following references: Edwards, A., Voss, H., Rice, P., Civitello, A., Stegemann, J., Schwager, C., Zinimermann, J., Erfle, H., Caskey, C..T. and Ansorge, W. (1990), Automated DNA Sequencing of the Human HPRT Locus, Genomics, 6:593-608; Gibbs, R., Nguyen, P.-N., Edwards, A., Civitello, A. and Caskey, C.T.
  • a high-density or ultra-high density microfabricated device is constructed and attachment of oligonucleotide probes is carried out within the bounded regions of the wafer. Included are the normal probes (1-18) plus the specific probes that correspond to five different known mutations, including the above mutations (sites 19 and 20, respectively).
  • the foregoing uses two sets of PCR primers (Table I) to amplify the exons 7/8 region of hamster genomic DNA.
  • a radioactive label ( 32 P) is incorporated into the PCR fragments during amplification, which enables detection of hybridization by autoradiography or phosphorimager.
  • Figure 5 illustrates the results when the above probes are attached at one end to the surface at specific test sites within the DNA chip (numbered as above). Idealized hybridization patterns for two of the mutants (10-base deletion on left and A-G transition on right) are shown at the bottom.
  • Part A Fabrication of Porous Silicon Wafer
  • EXAMPLE 3 The procedure outlined in EXAMPLE 3 for fabrication of a porous silicon wafer with integral sample wells is followed, to yield a wafer with a 50x50 array of 200 ⁇ m square patches of pores, spaced 400 ⁇ m apart (center-to-center) over the surface of the wafer.
  • the pores of the wafer are activated to bind amine-derivatized polynucleotides by reaction with epoxysilane, as described in EXAMPLE 4.
  • Part B Formation of cDNA Array
  • a set of 2,500 M13 clones, selected from a normalized human cDNA library, is subjected to the polymerase chain reaction (PCR) in the presence of 5'-hexylamine-dUTP to amplify the cDNA inserts and incorporate primary amines into the strands.
  • the PCR products are ethanol-precipitated, dissolved in water or 10mM KOH, heat-denatured at 100°C for 5 min., then quenched on ice and applied to individual sample wells of the porous wafer suing a Hamilton Microlab 2200 fluid delivery system equipped with an 8-needle dispensing head. After all cDNA fragments are dispensed, a slight vacuum is briefly applied from below to ensure that fluid has occupied the pores.
  • the porous wafer is flushed with warm water, then reacted with 50mM triethylamine to cap off the unreacted epoxy groups on the surface, then flushed again with warm water and air dried.
  • Cytoplasmic RNA is extracted from cultured cells by the method of Chomczynski and Sacchi ( Anal . Biochem.162 :156-159 (1993)), treated with DNAse I to remove DNA contamination, then extracted with phenol/chloroform and ethanol precipitated. Reverse transcriptions and PCR are performed as described in the "differential display" protocol of Nishio et al. ( FASEB J . 8 :103-106 (1994)). Prior to hybridization, PCR products are labeled by random priming in the presence of [A- 32 P]dNTPs, and unincorporated label is removed by Centricon filtration.
  • a solution of 1% "Blotto" or 50mM tripolyphosphate is flowed through the porous silicon wafer to minimize the nonspecific binding of target DNA, then the porous silicon array is washed with hybridization solution (50mM Tris-HCl, pH 7.5, 1mM EDTA, 1M NaCl). Labeled PCR fragments representing the 3'-end of expressed genes are recovered from the Centricon filtration units in hybridization buffer, and the entire porous wafer is flooded with this DNA solution.
  • the porous hybridization module is placed at 65 °C and a peristaltic pump, connected to the lower vacuum chamber, is used to gradually flow the labeled DNA through the pores of the wafer over the course of 30-60 minutes.
  • the porous wafer is washed three times with hybridization buffer at 65°C.
  • the porous wafer is briefly dried, then placed onto the phosphor screen of a phosphorimager and kept in the dark for a period of time determined by the intensity of label.
  • the phosphor screen is then placed into the phosphorimager reader for quantitation of individual hybridization signals arising from each porous region in the array.
  • Figure 6 illustrates results obtainable from a hybridization experiment.
  • Total cytoplasmic mRNA is isolated from cells cultured under two conditions and subjected to the "differential display" procedure described above to prepare fragments representative of individual mKNA species present under the two conditions. These samples are hybridized to two identical cDNA arrays, to yield the two hybridization signal patterns shown. These patterns represent the profile of expressed genes under the two different culture conditions (for example in the presence and absence of a drug or chemical that induces a change in the expression of some genes). Note that overall, the pattern of hybridization is similar for the two conditions, but as expected for a diffential expression of certain genes under the two conditions, there are a few hybridization signals that are seen only for culture condition 1 and a few that are seen only for culture condition 2.
  • the squares represent sites where hybridization has occurred and the darkness of the squares is proportional to the number of labeled fragments present

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ATE424927T1 (de) 2009-03-15
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US5843767A (en) 1998-12-01
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WO1995011755A1 (en) 1995-05-04
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EP0725682B1 (de) 2002-03-20
ATE214633T1 (de) 2002-04-15
AU700315B2 (en) 1998-12-24
EP0725682A1 (de) 1996-08-14
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ES2325393T3 (es) 2009-09-03
CA2174140A1 (en) 1995-05-04
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