DE69826697D1 - In vitro selektionsmethode - Google Patents

In vitro selektionsmethode

Info

Publication number
DE69826697D1
DE69826697D1 DE69826697T DE69826697T DE69826697D1 DE 69826697 D1 DE69826697 D1 DE 69826697D1 DE 69826697 T DE69826697 T DE 69826697T DE 69826697 T DE69826697 T DE 69826697T DE 69826697 D1 DE69826697 D1 DE 69826697D1
Authority
DE
Germany
Prior art keywords
nucleic acid
acid molecule
microcapsules
selection method
vitro selection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69826697T
Other languages
English (en)
Other versions
DE69826697T2 (de
Inventor
Andrew Griffiths
Dan Tawfik
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Medical Research Council
Original Assignee
Medical Research Council
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26311843&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69826697(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from GBGB9714300.2A external-priority patent/GB9714300D0/en
Priority claimed from GBGB9806393.6A external-priority patent/GB9806393D0/en
Application filed by Medical Research Council filed Critical Medical Research Council
Publication of DE69826697D1 publication Critical patent/DE69826697D1/de
Application granted granted Critical
Publication of DE69826697T2 publication Critical patent/DE69826697T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1062—Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
DE69826697T 1997-07-07 1998-06-29 In vitro selektionsmethode Expired - Lifetime DE69826697T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB9714300.2A GB9714300D0 (en) 1997-07-07 1997-07-07 In vitro sorting method
GB9714300 1997-07-07
GB9806393 1998-03-25
GBGB9806393.6A GB9806393D0 (en) 1998-03-25 1998-03-25 In vitro sorting method
PCT/GB1998/001889 WO1999002671A1 (en) 1997-07-07 1998-06-29 In vitro sorting method

Publications (2)

Publication Number Publication Date
DE69826697D1 true DE69826697D1 (de) 2004-11-04
DE69826697T2 DE69826697T2 (de) 2006-02-16

Family

ID=26311843

Family Applications (4)

Application Number Title Priority Date Filing Date
DE69841997T Expired - Lifetime DE69841997D1 (de) 1997-07-07 1998-06-29 In-vitro-Sortierverfahren
DE69838521T Expired - Lifetime DE69838521T2 (de) 1997-07-07 1998-06-29 Methode zur Erhöhung der Konzentration von Nucleinsäuremolekülen
DE69826697T Expired - Lifetime DE69826697T2 (de) 1997-07-07 1998-06-29 In vitro selektionsmethode
DE69837453T Expired - Lifetime DE69837453T2 (de) 1997-07-07 1998-06-29 Ein in vitro Sortierverfahren

Family Applications Before (2)

Application Number Title Priority Date Filing Date
DE69841997T Expired - Lifetime DE69841997D1 (de) 1997-07-07 1998-06-29 In-vitro-Sortierverfahren
DE69838521T Expired - Lifetime DE69838521T2 (de) 1997-07-07 1998-06-29 Methode zur Erhöhung der Konzentration von Nucleinsäuremolekülen

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69837453T Expired - Lifetime DE69837453T2 (de) 1997-07-07 1998-06-29 Ein in vitro Sortierverfahren

Country Status (13)

Country Link
US (7) US6489103B1 (de)
EP (6) EP1482036B1 (de)
JP (3) JP4298912B2 (de)
AT (4) ATE487790T1 (de)
AU (1) AU736836B2 (de)
CA (3) CA2584453A1 (de)
CY (1) CY1111537T1 (de)
DE (4) DE69841997D1 (de)
DK (3) DK1019496T3 (de)
ES (4) ES2402516T3 (de)
GB (1) GB2342094B (de)
PT (2) PT1801214E (de)
WO (1) WO1999002671A1 (de)

Families Citing this family (350)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7285416B2 (en) 2000-01-24 2007-10-23 Gendaq Limited Regulated gene expression in plants
US7262055B2 (en) 1998-08-25 2007-08-28 Gendaq Limited Regulated gene expression in plants
US20030027126A1 (en) 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
US7622294B2 (en) 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
DE69837913T2 (de) 1997-04-01 2008-02-07 Solexa Ltd., Saffron Walden Verfahren zur vervielfältigung von nukleinsäure
ATE487790T1 (de) 1997-07-07 2010-11-15 Medical Res Council In-vitro-sortierverfahren
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
AU2004242526B2 (en) * 1999-01-07 2007-08-30 United Kingdom Research And Innovation Optical Sorting Method
CA2365981A1 (en) 1999-03-24 2000-09-28 Board Of Regents, The University Of Texas System Linear and circular expression elements
GB9917027D0 (en) 1999-07-20 1999-09-22 Affibody Technology Sweeden Ab In vitro selection and optional identification of polypeptides using solid support carriers
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
GB0022458D0 (en) * 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
WO2002074952A2 (de) * 2001-03-16 2002-09-26 Rina Netzwerk Rna-Technologien Gmbh Gegen abbau stabilisierte nukleinsäure
JP4838957B2 (ja) * 2001-05-02 2011-12-14 独立行政法人理化学研究所 無細胞タンパク質合成系を用いたタンパク質の製造方法
GB0114854D0 (en) * 2001-06-18 2001-08-08 Medical Res Council Selective gene amplification
GB0115841D0 (en) 2001-06-28 2001-08-22 Medical Res Council Ligand
WO2003037302A1 (en) * 2001-10-30 2003-05-08 Windsor J Brian Method and system for the co-isolation of cognate dna, rna and protein sequences and method for screening co-isolates for defined activities
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
JP4359707B2 (ja) 2002-05-22 2009-11-04 独立行政法人理化学研究所 チオレドキシン融合タンパク質発現ベクターを用いた無細胞タンパク質合成系によるタンパク質の製造方法
AUPS298102A0 (en) * 2002-06-13 2002-07-04 Nucleics Pty Ltd Method for performing chemical reactions
JP2006507921A (ja) * 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
US20060002935A1 (en) 2002-06-28 2006-01-05 Domantis Limited Tumor Necrosis Factor Receptor 1 antagonists and methods of use therefor
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
PT1534830E (pt) 2002-09-06 2011-01-31 Isogenica Ltd Biblioteca de expressão de péptidos in vitro
GB0221053D0 (en) * 2002-09-11 2002-10-23 Medical Res Council Single-molecule in vitro evolution
JP4441170B2 (ja) 2002-11-28 2010-03-31 独立行政法人理化学研究所 Ｓ１２リボソームタンパク質に変異を有する大腸菌細胞抽出液及びそれを用いる無細胞系によるタンパク質の製造方法
GB0228819D0 (en) * 2002-12-11 2003-01-15 British American Tobacco Co Improvements relating to smoking articles
GB0230203D0 (en) 2002-12-27 2003-02-05 Domantis Ltd Fc fusion
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
DE602004024034D1 (de) * 2003-01-29 2009-12-24 454 Corp Nukleinsäureamplifikation auf basis von kügelchenemulsion
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307403D0 (en) * 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
GB0307428D0 (en) * 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
FI20030615A0 (fi) * 2003-04-24 2003-04-24 Marko Virta Nanopartikkeli bioaffiniteettimäärityksiin
EP3616781A1 (de) 2003-04-10 2020-03-04 President and Fellows of Harvard College Erzeugung und kontrolle von fluiden
EP1627062A1 (de) 2003-05-14 2006-02-22 Domantis Limited Verfahren zur gewinnung aus einem polypeptid - repertoire von polypeptiden, die sich reversibel entfalten
EP1641809B2 (de) 2003-07-05 2018-10-03 The Johns Hopkins University Verfahren und zusammensetzungen zum nachweis und zur auflistung genetischer variationen
CN104069784B (zh) 2003-08-27 2017-01-11 哈佛大学 流体物种的电子控制
EP1668126B1 (de) * 2003-10-01 2008-02-13 ETH Zürich Verfahren zur in vitro evolution von polypeptiden
GB0325653D0 (en) 2003-11-03 2003-12-10 Medical Res Council CST emulsions
WO2005049787A2 (en) * 2003-11-24 2005-06-02 Yeda Research And Development Co.Ltd. Compositions and methods for in vitro sorting of molecular and cellular libraries
EP1735458B1 (de) 2004-01-28 2013-07-24 454 Life Sciences Corporation Nukleinsäureamplifikation mit durchlaufemulsion
US20050221339A1 (en) * 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US9477233B2 (en) 2004-07-02 2016-10-25 The University Of Chicago Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets
GB0418651D0 (en) * 2004-08-20 2004-09-22 Medical Res Council Method
GB0419628D0 (en) 2004-09-03 2004-10-06 European Molecular Biology Lab Embl Method for determining protein solubility
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
CN101724071A (zh) 2004-10-08 2010-06-09 杜门蒂斯有限公司 抗肿瘤坏死因子受体1的单域抗体及其使用方法
US20080004436A1 (en) * 2004-11-15 2008-01-03 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Directed Evolution and Selection Using in Vitro Compartmentalization
JP4868731B2 (ja) 2004-11-17 2012-02-01 独立行政法人理化学研究所 哺乳動物培養細胞由来の無細胞タンパク質合成システム
JP4787488B2 (ja) 2004-11-19 2011-10-05 独立行政法人理化学研究所 直鎖状鋳型ｄｎａを用いた無細胞タンパク質合成方法及びそのための細胞抽出液
EP2233583A1 (de) 2005-02-01 2010-09-29 AB Advanced Genetic Analysis Corporation Nukleinsäuresequenzierung durch schrittweise Duplexverlängerung
EP2003214B1 (de) 2005-02-01 2013-04-10 AB Advanced Genetic Analysis Corporation Reagenzien, Verfahren und Bibliotheken für Partikel-basierte Sequenzierung
US20070054119A1 (en) * 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
EP2248578B1 (de) 2005-03-04 2012-06-06 President and Fellows of Harvard College Verfahren zur Bildung mehrerer Emulsionen
US20060228721A1 (en) * 2005-04-12 2006-10-12 Leamon John H Methods for determining sequence variants using ultra-deep sequencing
WO2006119066A2 (en) * 2005-04-29 2006-11-09 The J. Craig Venter Institute Amplification and cloning of single dna molecules using rolling circle amplification
US20090233291A1 (en) * 2005-06-06 2009-09-17 454 Life Sciences Corporation Paired end sequencing
WO2007145612A1 (en) 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
DK1907571T3 (en) 2005-06-15 2017-08-21 Complete Genomics Inc NUCLEIC ACID ANALYSIS USING INCIDENTAL MIXTURES OF NON-OVERLAPPING FRAGMENTS
US7723077B2 (en) 2005-08-11 2010-05-25 Synthetic Genomics, Inc. In vitro recombination method
JP5033949B2 (ja) * 2005-08-12 2012-09-26 国立大学法人豊橋技術科学大学 電気乳化法
WO2007024914A2 (en) * 2005-08-22 2007-03-01 Applera Corporation Device and method for microfluidic control of a first fluid in contact with a second fluid, wherein the first and second fluids are immiscible
EP1951900B1 (de) 2005-10-07 2016-06-15 Callida Genomics, Inc. Selbstzusammengesetzte einzelmolekül-arrays und verwendungen davon
KR100828714B1 (ko) 2005-10-25 2008-05-09 주식회사 엘지화학 리보핵산을 이용한 멀티플렉스 증폭방법
EP1801212A1 (de) * 2005-12-21 2007-06-27 Eidgenössische Technische Hochschule Zürich Selektion von Biokatalysatoren für die chemische Synthese
JP2009536313A (ja) 2006-01-11 2009-10-08 レインダンス テクノロジーズ， インコーポレイテッド ナノリアクターの形成および制御において使用するマイクロ流体デバイスおよび方法
CA2640024A1 (en) * 2006-01-27 2007-08-09 President And Fellows Of Harvard College Fluidic droplet coalescence
SG10201405158QA (en) 2006-02-24 2014-10-30 Callida Genomics Inc High throughput genome sequencing on dna arrays
CA2643700A1 (en) 2006-02-24 2007-11-22 Callida Genomics, Inc. High throughput genome sequencing on dna arrays
US20090062129A1 (en) * 2006-04-19 2009-03-05 Agencourt Personal Genomics, Inc. Reagents, methods, and libraries for gel-free bead-based sequencing
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2530168B1 (de) * 2006-05-11 2015-09-16 Raindance Technologies, Inc. Mikrofluidische Vorrichtungen
EP2636755A1 (de) * 2006-05-26 2013-09-11 AltheaDx Incorporated Biochemische Analyse partitionierter Zellen
WO2008021123A1 (en) 2006-08-07 2008-02-21 President And Fellows Of Harvard College Fluorocarbon emulsion stabilizing surfactants
WO2008042067A2 (en) 2006-09-28 2008-04-10 Illumina, Inc. Compositions and methods for nucleotide sequencing
US7910302B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US7682791B2 (en) * 2006-10-29 2010-03-23 Macevicz Stephen C Method of generating nested sets of double stranded DNA circles
US20090111705A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by hybrid capture
EP2121983A2 (de) 2007-02-02 2009-11-25 Illumina Cambridge Limited Verfahren zur kennzeichnung von proben und sequenzierung mehrerer nukleotidmatrizen
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
ATE490319T1 (de) 2007-02-23 2010-12-15 New England Biolabs Inc Auswahl und anreicherung von proteinen durch in- vitro-untergliederung
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
EP2121984A2 (de) * 2007-03-16 2009-11-25 454 Life Sciences Corporation System und verfahren zur erkennung von hiv-arzneiresistenten varianten
WO2008121342A2 (en) * 2007-03-28 2008-10-09 President And Fellows Of Harvard College Emulsions and techniques for formation
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
CA2689356A1 (en) * 2007-06-01 2008-12-11 454 Life Sciences Corporation System and meth0d for identification of individual samples from a multiplex mixture
US7883265B2 (en) * 2007-06-01 2011-02-08 Applied Biosystems, Llc Devices, systems, and methods for preparing emulsions
MX2009013137A (es) 2007-06-06 2010-04-30 Domantis Ltd Metodos para seleccionar polipeptidos resistentes a la proteasa.
CN101802218A (zh) * 2007-06-28 2010-08-11 454生命科学公司 在核酸测序中用于自适应试剂控制的系统和方法
EP2212434A1 (de) * 2007-10-01 2010-08-04 Applied Biosystems Inc. Chase-ligation-sequenzierung
EP2213747B1 (de) 2007-11-05 2021-06-30 Riken Verfahren zur herstellung eines membranproteins
WO2009067743A1 (en) 2007-11-27 2009-06-04 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
US20110008345A1 (en) 2007-11-30 2011-01-13 Claire Ashman Antigen-binding constructs
EP2235210B1 (de) 2007-12-21 2015-03-25 President and Fellows of Harvard College Verfahren zur nukleinsäuresequenzierung
EP2274441B1 (de) 2008-02-04 2012-06-20 Life Technologies Corporation Zusammensetzung und verfahren zum sequenzieren von nukleinsäure
US20090203086A1 (en) * 2008-02-06 2009-08-13 454 Life Sciences Corporation System and method for improved signal detection in nucleic acid sequencing
US20110020345A1 (en) 2008-03-31 2011-01-27 Christopher Herring Drug fusions and conjugates
MX2010010798A (es) 2008-04-04 2011-01-21 Allopartis Biotechnologies Inc Metodo para incrementar la actividad enzimatica.
GB0806562D0 (en) 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid
US7888034B2 (en) 2008-07-01 2011-02-15 454 Life Sciences Corporation System and method for detection of HIV tropism variants
EP2291533B2 (de) 2008-07-02 2020-09-30 Illumina Cambridge Limited Verwendung von kügelchengruppen zur herstellung von arrays auf oberflächen
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US8663920B2 (en) 2011-07-29 2014-03-04 Bio-Rad Laboratories, Inc. Library characterization by digital assay
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
WO2010038042A1 (en) 2008-10-02 2010-04-08 Illumina Cambridge Ltd. Nucleic acid sample enrichment for sequencing applications
US20100261189A1 (en) * 2008-10-03 2010-10-14 Roche Molecular Systems, Inc. System and method for detection of HLA Variants
US20100087325A1 (en) * 2008-10-07 2010-04-08 Illumina, Inc. Biological sample temperature control system and method
WO2010048337A2 (en) 2008-10-22 2010-04-29 Illumina, Inc. Preservation of information related to genomic dna methylation
CA2742298C (en) 2008-11-03 2019-09-10 The Regents Of The University Of California Methods for detecting modification resistant nucleic acids
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
EP2719774B8 (de) 2008-11-07 2020-04-22 Adaptive Biotechnologies Corporation Verfahren zur Überwachung von Zuständen durch Sequenzanalyse
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
MX2011005874A (es) 2008-12-05 2011-06-27 Glaxo Group Ltd Metodos para seleccionar polipeptidos resistentes a proteasa.
GB0822261D0 (en) 2008-12-05 2009-01-14 Merten Christoph Assay
GB0822260D0 (en) 2008-12-05 2009-01-14 Merten Christoph Assay
EP3290531B1 (de) 2008-12-19 2019-07-24 President and Fellows of Harvard College Partikelunterstützte nukleinsäuresequenzierung
US8236532B2 (en) 2008-12-23 2012-08-07 Illumina, Inc. Multibase delivery for long reads in sequencing by synthesis protocols
DK3059337T3 (da) 2009-01-15 2019-07-22 Adaptive Biotechnologies Corp Adaptive immunity profiling og metoder til frembringelse af monoklonale antibodier
US8581209B2 (en) * 2009-01-29 2013-11-12 Southwest Research Institute Fluorescent monitoring of microcapsule oxidation
CA2750477A1 (en) 2009-02-19 2010-08-26 Stephen Duffield Improved anti-tnfr1 polypeptides, antibody variable domains & antagonists
US20110305694A1 (en) 2009-02-24 2011-12-15 Paul Andrew Hamblin Multivalent and/or multispecific rankl-binding constructs
JP2012518399A (ja) 2009-02-24 2012-08-16 グラクソ グループ リミテッド 抗原結合性構築物
JP2012518398A (ja) 2009-02-24 2012-08-16 グラクソ グループ リミテッド 抗原結合性構築物
EP2406003A2 (de) 2009-03-13 2012-01-18 President and Fellows of Harvard College Massstabsvergrösserung von strömungsfokussierenden mikrofluidikvorrichtungen
GB0904957D0 (en) 2009-03-23 2009-05-06 Univ Erasmus Medical Ct Tumour gene profile
EP3415235A1 (de) 2009-03-23 2018-12-19 Raindance Technologies Inc. Manipulation von mikrofluidiktröpfchen
CN104127880A (zh) 2009-03-27 2014-11-05 葛兰素集团有限公司 药用融合体和缀合物
WO2010117457A2 (en) * 2009-04-08 2010-10-14 Applied Biosystems, Llc System comprising dual-sided thermal cycler and emulsion pcr in pouch
WO2010129541A1 (en) * 2009-05-04 2010-11-11 Affomix Corporation Emulsion selection of antibodies
KR20120044941A (ko) 2009-06-25 2012-05-08 프레드 헛친슨 켄서 리서치 센터 적응 면역의 측정방법
JP5099455B2 (ja) * 2009-07-16 2012-12-19 独立行政法人科学技術振興機構 エマルジョンを用いたｒｎａの選択的増幅法
EP2453920A2 (de) 2009-07-16 2012-05-23 Glaxo Group Limited Antagonisten, verwendung und verfahren zur teilweisen tnfr1-hemmung
US20120211084A1 (en) 2009-09-02 2012-08-23 President And Fellows Of Harvard College Multiple emulsions created using jetting and other techniques
EP2940153B1 (de) 2009-09-02 2020-05-13 Bio-Rad Laboratories, Inc. System zum Mischen von Flüssigkeiten durch Koaleszenz mehrerer Emulsionen
US8182994B2 (en) 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
MX2012003939A (es) 2009-09-30 2012-07-30 Glaxo Group Ltd Fusiones y conjugados de farmaco.
US8609339B2 (en) 2009-10-09 2013-12-17 454 Life Sciences Corporation System and method for emulsion breaking and recovery of biological elements
WO2011042564A1 (en) 2009-10-09 2011-04-14 Universite De Strasbourg Labelled silica-based nanomaterial with enhanced properties and uses thereof
GB0918564D0 (en) 2009-10-22 2009-12-09 Plasticell Ltd Nested cell encapsulation
US9056289B2 (en) 2009-10-27 2015-06-16 President And Fellows Of Harvard College Droplet creation techniques
AR078763A1 (es) 2009-10-27 2011-11-30 Glaxo Group Ltd Polipeptidos anti factor de necrosis tumoral 1 (anti-tnfr1) estables, dominios variables de anticuerpos y antagonistas
EP2494353B1 (de) 2009-10-30 2017-09-27 Illumina Inc. Vorrichtung mit einer Mehrzahl von codierten Mikrogefäßen und einer Vielzahl von Fächern und ein Verfahren zum Lesen einer Mehrzahl von codierten Mikrogefäßen
EP2333102A1 (de) * 2009-12-14 2011-06-15 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren zur Identifizierung von RNA-Liganden
US10837883B2 (en) 2009-12-23 2020-11-17 Bio-Rad Laboratories, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
WO2011086143A2 (en) 2010-01-14 2011-07-21 Glaxo Group Limited Liver targeting molecules
EP3151052A1 (de) 2010-02-01 2017-04-05 Illumina, Inc. Fokussierungsverfahren und optische systeme sowie anordnungen damit
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
JP5934657B2 (ja) 2010-02-12 2016-06-15 レインダンス テクノロジーズ， インコーポレイテッド デジタル検体分析
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US8481903B2 (en) 2010-03-06 2013-07-09 Alexander Triener Systems, methods, and apparatuses including a moveable optical component for detecting optical signals from a sample
WO2011116154A2 (en) * 2010-03-17 2011-09-22 President And Fellows Of Harvard College Melt emulsification
JP6155419B2 (ja) 2010-03-25 2017-07-05 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド 検出用の液滴輸送システム
JP2013524169A (ja) 2010-03-25 2013-06-17 クァンタライフ・インコーポレーテッド 液滴によるアッセイ用の検出システム
EP2550528B1 (de) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Tropfenerzeugung für tropfenbasierte prüfungen
CA2793970A1 (en) 2010-04-30 2011-11-03 F. Hoffmann-La Roche Ag System and method for purification and use of inorganic pyrophosphatase from aquifex aeolicus
WO2011143194A2 (en) 2010-05-10 2011-11-17 Life Technologies Corporation System and method for processing a biological sample
US20110287432A1 (en) 2010-05-21 2011-11-24 454 Life Science Corporation System and method for tailoring nucleotide concentration to enzymatic efficiencies in dna sequencing technologies
US9650629B2 (en) 2010-07-07 2017-05-16 Roche Molecular Systems, Inc. Clonal pre-amplification in emulsion
CN103153466B (zh) 2010-07-22 2016-04-13 基因细胞生物系统有限公司 复合液体池
US9309556B2 (en) 2010-09-24 2016-04-12 The Board Of Trustees Of The Leland Stanford Junior University Direct capture, amplification and sequencing of target DNA using immobilized primers
US8759038B2 (en) 2010-09-29 2014-06-24 Illumina Cambridge Limited Compositions and methods for sequencing nucleic acids
US20120077716A1 (en) 2010-09-29 2012-03-29 454 Life Sciences Corporation System and method for producing functionally distinct nucleic acid library ends through use of deoxyinosine
EP3447155A1 (de) 2010-09-30 2019-02-27 Raindance Technologies, Inc. Sandwichassays in tröpfchen
US20120322665A1 (en) 2010-10-08 2012-12-20 454 Life Sciences Corporation System and method for detection of hiv-1 clades and recombinants of the reverse transcriptase and protease regions
EP2633069B1 (de) 2010-10-26 2015-07-01 Illumina, Inc. Sequenzierungsverfahren
WO2012058647A1 (en) 2010-10-28 2012-05-03 Life Technologies Corporation Chemically-enhanced primer compositions, methods and kits
SG190074A1 (en) 2010-11-01 2013-06-28 Bio Rad Laboratories System for forming emulsions
EP2643484A4 (de) 2010-11-22 2014-04-16 Univ California Verfahren zur identifizierung eines rna-transkripts zellulären ursprungs
EP2643351A1 (de) 2010-11-24 2013-10-02 Glaxo Group Limited Auf hgf abzielende multispezifische antigenbindende proteine
WO2012073045A2 (en) 2010-12-03 2012-06-07 Cyclogenix Ltd Polypeptide scaffold
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
WO2012109600A2 (en) 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
EP2675819B1 (de) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Zusammensetzungen und verfahren für molekulare etikettierung
WO2012129187A1 (en) 2011-03-18 2012-09-27 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
US20120244523A1 (en) 2011-03-25 2012-09-27 454 Life Sciences Corporation System and Method for Detection of HIV Integrase Variants
WO2012136792A2 (en) 2011-04-07 2012-10-11 Glaxo Group Limited Cck compositions
WO2012136790A1 (en) 2011-04-07 2012-10-11 Glaxo Group Limited Compositions comprising fusion proteins or conjugates with an improved half -life
WO2012149042A2 (en) 2011-04-25 2012-11-01 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
GB201108259D0 (en) * 2011-05-17 2011-06-29 Cambridge Entpr Ltd Gel beads in microfluidic droplets
US9238206B2 (en) 2011-05-23 2016-01-19 President And Fellows Of Harvard College Control of emulsions, including multiple emulsions
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
EP2714970B1 (de) 2011-06-02 2017-04-19 Raindance Technologies, Inc. Enzymquantifizierung
ES2692894T3 (es) 2011-06-08 2018-12-05 Life Technologies Corporation Diseño y desarrollo de nuevos detergentes para su uso en sistemas de PCR
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
EP2729238A2 (de) 2011-07-06 2014-05-14 President and Fellows of Harvard College Multiple emulsionen und verfahren zur herstellung von multiplen emulsionen
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
KR20130023091A (ko) * 2011-08-26 2013-03-07 (주)바이오니아 단백질 합성 키트, 자동추출장비를 이용한 단백질 발현 및 추출 방법
JP5924888B2 (ja) * 2011-08-26 2016-05-25 関東化學株式会社 核酸抽出方法、核酸抽出試薬キットおよび核酸抽出用試薬
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
HRP20211523T1 (hr) 2011-09-23 2021-12-24 Illumina, Inc. Pripravci za sekvenciranje nukleinske kiseline
US10152569B2 (en) 2011-09-26 2018-12-11 Gen-Probe Incorporated Algorithms for sequence determinations
US10378051B2 (en) 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
DE102011054101A1 (de) 2011-09-30 2013-04-04 Albert-Ludwigs-Universität Freiburg Verfahren zur räumlichen Anordnung von Probenfragmenten zur Amplifikation und Immobilisierung für weitere Derivatisierungen
US9279159B2 (en) 2011-10-21 2016-03-08 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
DE102011085473A1 (de) 2011-10-28 2013-05-02 Albert-Ludwigs-Universität Freiburg Verfahren zur Identifikation von Aptameren
US8637242B2 (en) 2011-11-07 2014-01-28 Illumina, Inc. Integrated sequencing apparatuses and methods of use
GB201120711D0 (en) 2011-12-01 2012-01-11 Univ Erasmus Medical Ct Method for classifying tumour cells
US9200274B2 (en) 2011-12-09 2015-12-01 Illumina, Inc. Expanded radix for polymeric tags
EP3904536A1 (de) 2011-12-09 2021-11-03 Adaptive Biotechnologies Corporation Diagnose von lymphoid-malignität und nachweis minimaler resterkrankungen
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
CA2862364C (en) 2011-12-30 2021-02-23 Quest Diagnostics Investments Incorporated Nucleic acid analysis using emulsion pcr
KR102028225B1 (ko) 2012-01-25 2019-10-02 젠셀 바이오시스템즈 리미티드 생체분자 분리
US9701959B2 (en) 2012-02-02 2017-07-11 Invenra Inc. High throughput screen for biologically active polypeptides
US20130217023A1 (en) 2012-02-22 2013-08-22 454 Life Sciences Corporation System And Method For Generation And Use Of Compact Clonally Amplified Products
EP2823060B1 (de) 2012-03-05 2018-02-14 Adaptive Biotechnologies Corporation Festlegung von gepaarten immunrezeptorketten aus frequenzabgestimmten untereinheiten
NO2694769T3 (de) 2012-03-06 2018-03-03
US20130261984A1 (en) 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
JP5756247B1 (ja) 2012-05-08 2015-07-29 アダプティブ バイオテクノロジーズ コーポレイション マルチプレックスｐｃｒ反応における増幅バイアスを測定し校正する組成物及び方法
US10192024B2 (en) 2012-05-18 2019-01-29 454 Life Sciences Corporation System and method for generation and use of optimal nucleotide flow orders
EP2870264A4 (de) 2012-07-03 2016-03-02 Sloan Kettering Inst Cancer Quantitative beurteilung der wiederherstellung eines menschlichen t-zellen-repertoires nach der transplantation allogener blutbildender stammzellen
US9977861B2 (en) 2012-07-18 2018-05-22 Illumina Cambridge Limited Methods and systems for determining haplotypes and phasing of haplotypes
US10161007B2 (en) 2012-08-13 2018-12-25 The Regents Of The University Of California Methods and systems for detecting biological components
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9567631B2 (en) 2012-12-14 2017-02-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR102090851B1 (ko) 2012-08-14 2020-03-19 10엑스 제노믹스, 인크. 마이크로캡슐 조성물 및 방법
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
EP2904111B1 (de) 2012-10-01 2017-12-06 Adaptive Biotechnologies Corporation Immunkompetenzbeurteilung durch adaptive immunrezeptordiversität und klonalitätscharakterisierung
US10150996B2 (en) 2012-10-19 2018-12-11 Adaptive Biotechnologies Corp. Quantification of adaptive immune cell genomes in a complex mixture of cells
KR20150090037A (ko) 2012-11-27 2015-08-05 젠셀 바이오시스템즈 리미티드 액체 샘플의 취급
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN108753766A (zh) 2013-02-08 2018-11-06 10X基因组学有限公司 多核苷酸条形码生成
WO2014144822A2 (en) 2013-03-15 2014-09-18 Immumetrix, Inc. Methods and compositions for tagging and analyzing samples
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
US9348897B2 (en) 2013-08-07 2016-05-24 Google Inc. Method and system for providing scheduling suggestions
EP2840148B1 (de) 2013-08-23 2019-04-03 F. Hoffmann-La Roche AG Verfahren für nukleinsäureamplifikation
EP2848698A1 (de) 2013-08-26 2015-03-18 F. Hoffmann-La Roche AG System und Methode zur automatisierten Nukleinsäure-Amplifikation
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
EP3063129B1 (de) 2013-10-25 2019-04-17 Life Technologies Corporation Neuartige verbindungen zur verwendung in pcr-systemen und anwendungen davon
US10088973B2 (en) * 2013-11-08 2018-10-02 Google Llc Event scheduling presentation in a graphical user interface environment
WO2015069634A1 (en) 2013-11-08 2015-05-14 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
WO2015103367A1 (en) 2013-12-31 2015-07-09 Raindance Technologies, Inc. System and method for detection of rna species
US10384187B2 (en) 2014-02-10 2019-08-20 Gencell Biosystems Ltd Composite liquid cell (CLC) mediated nucleic acid library preparation device, and methods for using the same
WO2015134787A2 (en) 2014-03-05 2015-09-11 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
CN106413896B (zh) 2014-04-10 2019-07-05 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
EP3889325A1 (de) 2014-06-26 2021-10-06 10X Genomics, Inc. Verfahren zur analyse von nukleinsäuren aus einzelzellen oder zellpopulationen
US10839939B2 (en) 2014-06-26 2020-11-17 10X Genomics, Inc. Processes and systems for nucleic acid sequence assembly
US10697007B2 (en) 2014-06-27 2020-06-30 The Regents Of The University Of California PCR-activated sorting (PAS)
US20160085910A1 (en) 2014-09-18 2016-03-24 Illumina, Inc. Methods and systems for analyzing nucleic acid sequencing data
CN107278234A (zh) 2014-10-03 2017-10-20 生命科技公司 基因序列校验组合物、方法和试剂盒
CA3001986C (en) 2014-10-22 2023-02-21 The Regents Of The University Of California High definition microdroplet printer
MX2017005267A (es) 2014-10-29 2017-07-26 10X Genomics Inc Metodos y composiciones para la secuenciacion de acidos nucleicos seleccionados como diana.
WO2016069886A1 (en) 2014-10-29 2016-05-06 Adaptive Biotechnologies Corporation Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
EP3498866A1 (de) 2014-11-25 2019-06-19 Adaptive Biotechnologies Corp. Charakterisierung von adaptiver immunreaktion auf impfungen oder infektionen unter verwendung von immunrepertoiresequenzierung
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
KR20170106979A (ko) 2015-01-13 2017-09-22 10엑스 제노믹스, 인크. 구조 변이 및 위상 조정 정보를 시각화하기 위한 시스템 및 방법
JP2018508198A (ja) 2015-02-04 2018-03-29 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 別個の実体におけるバーコード付加による核酸のシーケンシング
JP2018513445A (ja) 2015-02-09 2018-05-24 １０エックス ゲノミクス，インコーポレイテッド 構造変異の特定及びバリアントコールデータを用いたフェージングのためのシステム及び方法
WO2016138122A1 (en) 2015-02-24 2016-09-01 Adaptive Biotechnologies Corp. Methods for diagnosing infectious disease and determining hla status using immune repertoire sequencing
EP3262188B1 (de) 2015-02-24 2021-05-05 10X Genomics, Inc. Verfahren zur sequenzierung von nukleinsäuren in gesteuertem ausmass
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
WO2016150771A1 (en) 2015-03-20 2016-09-29 Novozymes A/S Droplet-based selection by injection
DK3274692T3 (da) 2015-03-24 2022-08-29 Illumina Inc Fremgangsmåder til billeddannelse af prøver til biologisk eller kemisk analyse
AU2016242967B2 (en) 2015-04-01 2021-07-01 Adaptive Biotechnologies Corp. Method of identifying human compatible T cell receptors specific for an antigenic target
CN107864658A (zh) * 2015-04-07 2018-03-30 诺维信公司 用于选择具有脂肪酶活性的酶的方法
WO2016164583A2 (en) 2015-04-07 2016-10-13 Novozymes A/S Methods for selecting enzymes having enhanced activity
JP6698708B2 (ja) 2015-06-09 2020-05-27 ライフ テクノロジーズ コーポレーション 分子タグ付けのための方法、システム、組成物、キット、装置、及びコンピュータ可読媒体
CA3032514C (en) 2015-07-31 2021-05-04 Takehiro SHINODA Method of producing membrane protein and utilization thereof
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
US11123297B2 (en) 2015-10-13 2021-09-21 President And Fellows Of Harvard College Systems and methods for making and using gel microspheres
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
JP6954899B2 (ja) 2015-12-04 2021-10-27 １０エックス ゲノミクス，インコーポレイテッド 核酸の解析のための方法及び組成物
JP6735348B2 (ja) 2016-02-11 2020-08-05 １０エックス ジェノミクス， インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
WO2017177153A1 (en) 2016-04-07 2017-10-12 Novozymes A/S Methods for selecting enzymes having protease activity
CN109072480A (zh) * 2016-04-29 2018-12-21 生物辐射实验室股份有限公司 数字聚合酶保真度测定
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US11142791B2 (en) 2016-08-10 2021-10-12 The Regents Of The University Of California Combined multiple-displacement amplification and PCR in an emulsion microdroplet
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
WO2018064116A1 (en) 2016-09-28 2018-04-05 Illumina, Inc. Methods and systems for data compression
IL290157B2 (en) 2016-10-14 2023-09-01 Illumina Inc cartridge kit
US20200087658A1 (en) 2016-12-19 2020-03-19 Editas Medicine, Inc. Emulsion-based screening methods
CA3047328A1 (en) 2016-12-21 2018-06-28 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
GB201707143D0 (en) 2017-05-04 2017-06-21 Plasticell Ltd Method for producing cells
CN110870018A (zh) 2017-05-19 2020-03-06 10X基因组学有限公司 用于分析数据集的系统和方法
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10501739B2 (en) 2017-10-18 2019-12-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
WO2019076955A1 (en) 2017-10-18 2019-04-25 Novozymes A/S IMMUNOLOGICAL ASSAY FOR THE STABILITY OF A BIO-ACTIVE POLYPEPTIDE
EP3697531B1 (de) 2017-10-20 2022-07-27 Stilla Technologies Emulsionen mit verbesserter stabilität
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
CN111479631B (zh) 2017-10-27 2022-02-22 10X基因组学有限公司 用于样品制备和分析的方法和系统
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
CN111684064A (zh) 2018-01-19 2020-09-18 生物辐射实验室股份有限公司 突变dna聚合酶
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
EP3775271A1 (de) 2018-04-06 2021-02-17 10X Genomics, Inc. Systeme und verfahren zur qualitätskontrolle in einer einzelzellenverarbeitung
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
CA3122494A1 (en) 2018-12-13 2020-06-18 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020168013A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
WO2020185791A1 (en) 2019-03-11 2020-09-17 10X Genomics, Inc. Systems and methods for processing optically tagged beads
CA3138806A1 (en) 2019-05-22 2020-11-26 Dalia Dhingra Method and apparatus for simultaneous targeted sequencing of dna, rna and protein
US11667954B2 (en) 2019-07-01 2023-06-06 Mission Bio, Inc. Method and apparatus to normalize quantitative readouts in single-cell experiments
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
EP4277740A1 (de) 2021-01-13 2023-11-22 Pacific Biosciences of California, Inc. Oberflächenstrukturierung mit kolloidaler anordnung
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
WO2023122363A1 (en) 2021-12-23 2023-06-29 Illumina Software, Inc. Dynamic graphical status summaries for nucelotide sequencing
WO2023129764A1 (en) 2021-12-29 2023-07-06 Illumina Software, Inc. Automatically switching variant analysis model versions for genomic analysis applications
WO2023225488A1 (en) 2022-05-16 2023-11-23 Enkefalos Biosciences, Inc. Blood-brain barrier translocating peptides and related molecules and methods of use thereof
WO2024072614A1 (en) 2022-09-27 2024-04-04 Nautilus Subsidiary, Inc. Polypeptide capture, in situ fragmentation and identification

Family Cites Families (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2747570A (en) * 1954-05-19 1956-05-29 Jobst Conrad Means for assisting return circulation of fluids in an animal body
US3826249A (en) * 1973-01-30 1974-07-30 A Lee Leg constricting apparatus
US3908642A (en) * 1973-10-29 1975-09-30 Pred Vinmont Means for aerating and applying air pulsations within casts
US4206751A (en) * 1978-03-31 1980-06-10 Minnesota Mining And Manufacturing Company Intermittent compression device
US4801529A (en) * 1985-06-18 1989-01-31 Brandeis University Methods for isolating mutant microoganisms using microcapsules coated with indicator material
US5099030A (en) * 1987-03-09 1992-03-24 The Procter & Gamble Company Novel compounds, pharmaceutical compositions, and methods for treating inflammation and pain
CA2067194C (en) * 1989-10-05 2003-03-18 Glenn Kawasaki Cell-free synthesis and isolation of novel genes and polypeptides
ATE188509T1 (de) * 1990-02-16 2000-01-15 Hoffmann La Roche Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion
ES2030639T1 (es) 1990-09-21 1992-11-16 Bioplex Medical B.V. Dispositivo para colocar material estiptico, en concreto de forma fibrosa o esponjiforme sobre vasos sanguineos perforados, en concreto arterias.
US5955341A (en) * 1991-04-10 1999-09-21 The Scripps Research Institute Heterodimeric receptor libraries using phagemids
GB9107628D0 (en) 1991-04-10 1991-05-29 Moonbrook Limited Preparation of diagnostic agents
JPH06509473A (ja) * 1991-08-10 1994-10-27 メディカル・リサーチ・カウンシル 細胞個体群の処理
EP0608370B1 (de) 1991-10-15 1998-01-07 Multilyte Limited Bindungstest unter benutzung eines markierten reagens
US5270170A (en) * 1991-10-16 1993-12-14 Affymax Technologies N.V. Peptide library and screening method
US5413924A (en) 1992-02-13 1995-05-09 Kosak; Kenneth M. Preparation of wax beads containing a reagent for release by heating
DE4223169C1 (de) 1992-07-10 1993-11-25 Ferring Arzneimittel Gmbh Verfahren zur Mikroverkapselung wasserlöslicher Wirkstoffe
RU2048522C1 (ru) * 1992-10-14 1995-11-20 Институт белка РАН Способ размножения нуклеиновых кислот, способ их экспрессии и среда для их осуществления
IL104384A (en) * 1993-01-13 1996-11-14 Yeda Res & Dev Method for screening catalytic non-enzyme polypeptides and proteins
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
AU6707194A (en) 1993-04-19 1994-11-08 Medisorb Technologies International L.P. Encapsulation of nucleic acids with conjugates that facilitate and target cellular uptake and gene expression
CA2160457A1 (en) * 1993-04-19 1994-10-27 Stuart A. Kauffman Random chemistry for the generation of new compounds
AU8124694A (en) * 1993-10-29 1995-05-22 Affymax Technologies N.V. In vitro peptide and antibody display libraries
DE4402038A1 (de) 1994-01-25 1995-07-27 Borries Horst Von Durchdrückpackung
WO1995024929A2 (en) 1994-03-15 1995-09-21 Brown University Research Foundation Polymeric gene delivery system
US5508348A (en) * 1994-08-12 1996-04-16 The Research Foundation Of Suny Inverted emulsion pathways to conductive polymers
US5680283A (en) 1994-09-30 1997-10-21 Kabushiki Kaisha Toshiba Magnetic head and magnetic disk drive
US5824485A (en) 1995-04-24 1998-10-20 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US5910408A (en) * 1995-06-07 1999-06-08 The General Hospital Corporation Catalytic DNA having ligase activity
US5756122A (en) 1995-06-07 1998-05-26 Georgetown University Liposomally encapsulated nucleic acids having high entrapment efficiencies, method of manufacturer and use thereof for transfection of targeted cells
US5989892A (en) 1995-06-14 1999-11-23 Tonen Corporation Microorganisms, demulsifiers and processes for breaking an emulsion
AU6691496A (en) 1995-08-01 1997-02-26 Advanced Therapies, Inc. Enhanced artificial viral envelopes for cellular delivery of therapeutic substances
DE19646372C1 (de) * 1995-11-11 1997-06-19 Evotec Biosystems Gmbh Genotyp und Phänotyp koppelnde Verbindung
GB9608540D0 (en) 1996-04-25 1996-07-03 Medical Res Council Isolation of enzymes
US5876711A (en) * 1996-05-31 1999-03-02 Onyx Pharmaceuticals, Inc. Methods and compositions for determining the tumor suppressor status of cells
US6083693A (en) 1996-06-14 2000-07-04 Curagen Corporation Identification and comparison of protein-protein interactions that occur in populations
US5876771A (en) 1996-06-20 1999-03-02 Tetra Laval Holdings & Finance, Sa Process and article for determining the residence time of a food particle
WO1998013502A2 (en) 1996-09-27 1998-04-02 Icos Corporation Method to identify compounds for disrupting protein/protein interactions
US6395524B2 (en) 1996-11-27 2002-05-28 University Of Washington Thermostable polymerases having altered fidelity and method of identifying and using same
US6310354B1 (en) 1996-12-03 2001-10-30 Erkki Soini Method and a device for monitoring nucleic acid amplification reactions
ATE332368T1 (de) 1997-01-21 2006-07-15 Gen Hospital Corp Selektion von proteinen mittels rns-protein fusionen
CA2196496A1 (en) 1997-01-31 1998-07-31 Stephen William Watson Michnick Protein fragment complementation assay for the detection of protein-protein interactions
GB9703369D0 (en) * 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
AU6571598A (en) 1997-03-18 1998-10-12 Chromaxome Corporation Methods for screening compounds using encapsulated cells
US6048551A (en) * 1997-03-27 2000-04-11 Hilfinger; John M. Microsphere encapsulation of gene transfer vectors
ATE487790T1 (de) 1997-07-07 2010-11-15 Medical Res Council In-vitro-sortierverfahren
JP2981547B1 (ja) 1998-07-02 1999-11-22 農林水産省食品総合研究所長 クロスフロー型マイクロチャネル装置及び同装置を用いたエマルションの生成または分離方法
EP1100889A4 (de) 1998-07-17 2002-03-06 Mirus Corp Micell systeme
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
GB9921155D0 (en) 1999-09-08 1999-11-10 Medical Res Council Selection system
CN1189159C (zh) 2000-05-05 2005-02-16 欧莱雅 含水溶性美容活性组分水性核的微胶囊及含其的组合物
GB0022458D0 (en) 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
US6936264B2 (en) 2001-03-05 2005-08-30 The Procter & Gamble Company Delivery of reactive agents via multiple emulsions for use in shelf stable products
JP3570714B2 (ja) 2001-05-24 2004-09-29 株式会社リコー 現像剤収納容器及び画像形成装置
GB0114856D0 (en) 2001-06-18 2001-08-08 Medical Res Council Selection by avidity capture
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
GB0221053D0 (en) * 2002-09-11 2002-10-23 Medical Res Council Single-molecule in vitro evolution
AU2003302770B2 (en) 2002-12-20 2007-07-12 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA
DE602004024034D1 (de) 2003-01-29 2009-12-24 454 Corp Nukleinsäureamplifikation auf basis von kügelchenemulsion

Also Published As

Publication number Publication date
US7638276B2 (en) 2009-12-29
DE69837453D1 (de) 2007-05-10
US9528106B2 (en) 2016-12-27
JP2001509380A (ja) 2001-07-24
EP1908832A2 (de) 2008-04-09
EP2258846A2 (de) 2010-12-08
JP4156009B2 (ja) 2008-09-24
DE69838521D1 (de) 2007-11-15
PT1496120E (pt) 2007-04-30
JP4299255B2 (ja) 2009-07-22
CA2792122A1 (en) 1999-01-21
JP2005192572A (ja) 2005-07-21
DE69837453T2 (de) 2007-12-20
ATE358177T1 (de) 2007-04-15
JP2007143563A (ja) 2007-06-14
DE69838521T2 (de) 2008-05-21
GB9929447D0 (en) 2000-02-09
CA2295324A1 (en) 1999-01-21
ES2294427T3 (es) 2008-04-01
DE69826697T2 (de) 2006-02-16
US20070259374A1 (en) 2007-11-08
CA2295324C (en) 2012-12-18
EP1019496B1 (de) 2004-09-29
ES2283936T3 (es) 2007-11-01
EP1908832A3 (de) 2009-05-20
DK1801214T3 (da) 2011-01-24
EP1801214A3 (de) 2009-05-20
GB2342094B (en) 2002-10-23
AU8123198A (en) 1999-02-08
US20130190189A1 (en) 2013-07-25
WO1999002671A1 (en) 1999-01-21
CA2792122C (en) 2015-09-08
ATE487790T1 (de) 2010-11-15
EP1801214B1 (de) 2010-11-10
PT1801214E (pt) 2011-01-20
US8367326B2 (en) 2013-02-05
US20030124586A1 (en) 2003-07-03
US20050069920A1 (en) 2005-03-31
EP1482036B1 (de) 2007-10-03
EP1482036A2 (de) 2004-12-01
US7582446B2 (en) 2009-09-01
US7138233B2 (en) 2006-11-21
US20050042648A1 (en) 2005-02-24
EP1801214A2 (de) 2007-06-27
DK1019496T3 (da) 2005-01-10
CA2584453A1 (en) 1999-01-21
EP1496120A2 (de) 2005-01-12
ATE374815T1 (de) 2007-10-15
EP1496120A3 (de) 2005-05-11
ATE278013T1 (de) 2004-10-15
CY1111537T1 (el) 2015-08-05
EP1908832B1 (de) 2012-12-26
ES2402516T3 (es) 2013-05-06
EP1019496A1 (de) 2000-07-19
JP4298912B2 (ja) 2009-07-22
US7252943B2 (en) 2007-08-07
GB2342094A (en) 2000-04-05
EP2258846A3 (de) 2012-03-21
ES2230701T3 (es) 2005-05-01
US6489103B1 (en) 2002-12-03
DK1496120T3 (da) 2007-07-30
DE69841997D1 (de) 2010-12-23
EP1482036A3 (de) 2004-12-08
US20070077579A1 (en) 2007-04-05
AU736836B2 (en) 2001-08-02
EP1496120B1 (de) 2007-03-28

Similar Documents

Publication Publication Date Title
DE69826697D1 (de) In vitro selektionsmethode
DK1141272T3 (da) Optisk sorteringsmetode
DK1446504T3 (da) Emulsionssammensætninger
EP1505151A3 (de) Methode zur zielgerichteten Evolution
NO950381L (no) Nukleinsyresekvens amplifikasjon
ES2156861T3 (es) Secuenciacion ciclica de dna.
ES2136137T3 (es) Controles internos para reacciones de amplificacion isotermica de acidos nucleicos.
ATE185378T1 (de) Amplifikation von test reporters durch nukleinsäure replikation

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
R082 Change of representative

Ref document number: 1019496

Country of ref document: EP

Representative=s name: WSL PATENTANWAELTE PARTNERSCHAFTSGESELLSCHAFT, 651