ES2136137T3 - Controles internos para reacciones de amplificacion isotermica de acidos nucleicos. - Google Patents
Controles internos para reacciones de amplificacion isotermica de acidos nucleicos.Info
- Publication number
- ES2136137T3 ES2136137T3 ES94106632T ES94106632T ES2136137T3 ES 2136137 T3 ES2136137 T3 ES 2136137T3 ES 94106632 T ES94106632 T ES 94106632T ES 94106632 T ES94106632 T ES 94106632T ES 2136137 T3 ES2136137 T3 ES 2136137T3
- Authority
- ES
- Spain
- Prior art keywords
- amplification reactions
- nucleic acids
- internal controls
- isothermic amplification
- isothermic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
METODOS QUE EMPLEAN ESTANDARES INTERNOS DE OLIGONUCLEOTIDOS EN LAS REACCIONES ISOTERMICAS DE AMPLIFICACION DE ACIDO NUCLEICO PARA DETERMINAR LA EFICACIA DE LA REACCION DE AMPLIFICACION Y PARA CUANTIFICAR LOS NIVELES DE ACIERTO DE PREAMPLIFICACION.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/058,648 US5457027A (en) | 1993-05-05 | 1993-05-05 | Internal controls for isothermal nucleic acid amplification reactions |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2136137T3 true ES2136137T3 (es) | 1999-11-16 |
Family
ID=22018080
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES94106632T Expired - Lifetime ES2136137T3 (es) | 1993-05-05 | 1994-04-28 | Controles internos para reacciones de amplificacion isotermica de acidos nucleicos. |
Country Status (8)
Country | Link |
---|---|
US (2) | US5457027A (es) |
EP (1) | EP0623682B1 (es) |
JP (1) | JP3127079B2 (es) |
AU (1) | AU675503B2 (es) |
CA (1) | CA2121658C (es) |
DE (1) | DE69420454T2 (es) |
ES (1) | ES2136137T3 (es) |
SG (1) | SG50707A1 (es) |
Families Citing this family (46)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ZA936015B (en) * | 1992-08-24 | 1994-03-10 | Akzo Nv | Elimination of false negatives in nuleic acid detection. |
US5470723A (en) | 1993-05-05 | 1995-11-28 | Becton, Dickinson And Company | Detection of mycobacteria by multiplex nucleic acid amplification |
US5457027A (en) * | 1993-05-05 | 1995-10-10 | Becton, Dickinson And Company | Internal controls for isothermal nucleic acid amplification reactions |
AT401270B (de) * | 1994-09-26 | 1996-07-25 | Immuno Ag | Verfahren zur quantifizierung von genomischer dna |
CA2223241C (en) * | 1995-06-07 | 2009-11-17 | Gen-Probe Incorporated | Methods and kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
US5705365A (en) * | 1995-06-07 | 1998-01-06 | Gen-Probe Incorporated | Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
US5710029A (en) * | 1995-06-07 | 1998-01-20 | Gen-Probe Incorporated | Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
US5882867A (en) * | 1995-06-07 | 1999-03-16 | Dade Behring Marburg Gmbh | Detection of nucleic acids by formation of template-dependent product |
US5736333A (en) * | 1996-06-04 | 1998-04-07 | The Perkin-Elmer Corporation | Passive internal references for the detection of nucleic acid amplification products |
US6558901B1 (en) | 1997-05-02 | 2003-05-06 | Biomerieux Vitek | Nucleic acid assays |
IL124275A (en) * | 1997-05-02 | 2002-03-10 | Bio Merieux Vitek Inc | A method to produce sequences of nucleic acids |
US6289229B1 (en) | 1998-01-20 | 2001-09-11 | Scimed Life Systems, Inc. | Readable probe array for in vivo use |
US6365346B1 (en) * | 1998-02-18 | 2002-04-02 | Dade Behring Inc. | Quantitative determination of nucleic acid amplification products |
US6066458A (en) * | 1998-05-18 | 2000-05-23 | Becton, Dickinson And Company | Methods, apparatus and computer program products for determining quantities of nucleic acid sequences in samples using standard curves and amplification ratio estimates |
US8012719B2 (en) | 1998-11-17 | 2011-09-06 | Paolo Lusso | Method for the quantitative detection of nucleic acids |
IT1303767B1 (it) | 1998-11-17 | 2001-02-23 | San Raffaele Centro Fond | Metodo di quantificazione di acidi nucleici. |
WO2000041524A2 (en) * | 1999-01-11 | 2000-07-20 | President And Fellows Of Harvard College | Isothermal amplification of dna |
US6235479B1 (en) | 1999-04-13 | 2001-05-22 | Bio Merieux, Inc. | Methods and devices for performing analysis of a nucleic acid sample |
US6294338B1 (en) | 1999-07-23 | 2001-09-25 | Gen-Probe Incorporated | Polynucleotide amplification method |
EP1307587A2 (en) * | 2000-03-03 | 2003-05-07 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES | MULTIPLEX HYBRIDIZATION SYSTEM FOR IDENTIFICATION OF PATHOGENIC i MYCOBACTERIUM /i AND METHOD OF USE |
EP1236805B1 (en) * | 2001-03-02 | 2008-05-07 | Roche Diagnostics GmbH | A method for the determination of a nucleic acid using a control |
EP1236804A1 (en) * | 2001-03-02 | 2002-09-04 | Boehringer Mannheim Gmbh | A method for determination of a nucleic acid using a control |
AU2002323213B2 (en) * | 2001-08-16 | 2008-03-13 | Stratagene California | Compositions and methods comprising control nucleic acid |
AU2003208959A1 (en) * | 2002-01-30 | 2003-09-02 | Id Biomedical Corporation | Methods for detecting vancomycin-resistant microorganisms and compositions therefor |
ES2339240T3 (es) * | 2002-07-26 | 2010-05-18 | Abbott Laboratories | Metodo para detectar y cuantificar el virus de la hepatitis c. |
US20040024193A1 (en) * | 2002-07-26 | 2004-02-05 | Williams Gregg T. | Method of detecting and quantifying hepatitis C virus |
WO2004090160A1 (en) * | 2003-04-11 | 2004-10-21 | Dna Landmarks Inc. | Methods for relative quantification of specific nucleic acid sequences |
TWI375796B (en) | 2003-04-18 | 2012-11-01 | Becton Dickinson Co | Immuno-amplification |
WO2004097046A1 (en) * | 2003-05-01 | 2004-11-11 | Isis Innovation Limited | Stable internal standard for pcr and rt- pcr reactions |
US7229800B2 (en) * | 2004-04-16 | 2007-06-12 | Becton, Dickinson And Company | Neisseria gonorrhoeae assay |
US20060068380A1 (en) * | 2004-09-30 | 2006-03-30 | Gen-Probe Incorporated | Assay for detecting and quantifying HIV-1 |
EP2333561A3 (en) | 2005-03-10 | 2014-06-11 | Gen-Probe Incorporated | System for performing multi-formatted assays |
CA2670258C (en) | 2006-11-30 | 2020-09-08 | Gen-Probe Incorporated | Quantitative method employing adjustment of pre-defined master calibration curves |
US20080274458A1 (en) * | 2007-05-01 | 2008-11-06 | Latham Gary J | Nucleic acid quantitation methods |
US9689031B2 (en) | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
JP5822824B2 (ja) * | 2009-06-04 | 2015-11-24 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | 複合核酸の増幅 |
CA3155334A1 (en) | 2010-10-22 | 2012-04-26 | T2 Biosystems, Inc. | NMR SYSTEMS AND METHODS FOR RAPID ANALYTE DETECTION |
US8563298B2 (en) | 2010-10-22 | 2013-10-22 | T2 Biosystems, Inc. | NMR systems and methods for the rapid detection of analytes |
JP6169603B2 (ja) | 2011-12-22 | 2017-07-26 | サントル、ナショナール、ド、ラ、ルシェルシュ、シアンティフィク、(セーエヌエルエス) | 単一分子のハイブリダイゼーションおよび操作によるdnaの検出および定量法 |
US9932628B2 (en) | 2012-07-27 | 2018-04-03 | Gen-Probe Incorporated | Dual reference calibration method and system for quantifying polynucleotides |
US20140193819A1 (en) * | 2012-10-31 | 2014-07-10 | Becton, Dickinson And Company | Methods and compositions for modulation of amplification efficiency |
RU2713112C2 (ru) | 2013-04-01 | 2020-02-03 | Бектон, Дикинсон Энд Компани | Способы и средства для диагностики гриппа |
CA2965849A1 (en) * | 2014-12-16 | 2016-06-23 | Garvan Institute Of Medical Research | Sequencing controls |
US11326213B2 (en) | 2015-01-21 | 2022-05-10 | T2 Biosystems, Inc. | NMR methods and systems for the rapid detection of tick-borne pathogens |
WO2017127727A1 (en) | 2016-01-21 | 2017-07-27 | T2 Biosystems, Inc. | Rapid antimicrobial susceptibility testing using high-sensitivity direct detection methods |
AU2017250205A1 (en) | 2016-04-14 | 2018-11-22 | T2 Biosystems, Inc. | Methods and systems for amplification in complex samples |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2058893T3 (es) * | 1989-03-03 | 1994-11-01 | Genentech Inc | Procedimientos mejorados para la amplificacion de adn in vitro y para clonacion y cartografia de genomas. |
US5219727A (en) * | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
GB9016163D0 (en) * | 1990-07-24 | 1990-09-05 | Cemu Bioteknik | Chemical method |
WO1992011273A1 (en) * | 1990-12-20 | 1992-07-09 | F.Hoffmann-La Roche Ag | Pcr primers for detection of legionella species and methods for controlling visual intensity in hybridization assays |
GB9116042D0 (en) * | 1991-07-24 | 1991-09-11 | Royal Free Hosp School Med | Diagnostic method |
ES2214472T3 (es) * | 1991-08-02 | 2004-09-16 | Biomerieux B.V. | Cuantificacion de acidos nucleicos. |
US5457027A (en) * | 1993-05-05 | 1995-10-10 | Becton, Dickinson And Company | Internal controls for isothermal nucleic acid amplification reactions |
-
1993
- 1993-05-05 US US08/058,648 patent/US5457027A/en not_active Expired - Fee Related
-
1994
- 1994-04-19 CA CA002121658A patent/CA2121658C/en not_active Expired - Fee Related
- 1994-04-20 AU AU60580/94A patent/AU675503B2/en not_active Ceased
- 1994-04-28 JP JP06092181A patent/JP3127079B2/ja not_active Expired - Fee Related
- 1994-04-28 ES ES94106632T patent/ES2136137T3/es not_active Expired - Lifetime
- 1994-04-28 EP EP94106632A patent/EP0623682B1/en not_active Expired - Lifetime
- 1994-04-28 SG SG1996009243A patent/SG50707A1/en unknown
- 1994-04-28 DE DE69420454T patent/DE69420454T2/de not_active Expired - Lifetime
-
1997
- 1997-02-18 US US08/801,542 patent/US5840487A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CA2121658C (en) | 1996-12-03 |
US5457027A (en) | 1995-10-10 |
EP0623682A1 (en) | 1994-11-09 |
AU6058094A (en) | 1994-11-10 |
DE69420454T2 (de) | 1999-12-23 |
DE69420454D1 (de) | 1999-10-14 |
JPH06343497A (ja) | 1994-12-20 |
SG50707A1 (en) | 1998-07-20 |
CA2121658A1 (en) | 1994-11-06 |
JP3127079B2 (ja) | 2001-01-22 |
US5840487A (en) | 1998-11-24 |
EP0623682B1 (en) | 1999-09-08 |
AU675503B2 (en) | 1997-02-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ES2136137T3 (es) | Controles internos para reacciones de amplificacion isotermica de acidos nucleicos. | |
DE69826697D1 (de) | In vitro selektionsmethode | |
ATE345682T1 (de) | In vitro verfahren zum einbringen von nukleinsäuren in eine zelle | |
DE69534568D1 (de) | Säugetier-telomerase | |
FI880994A (fi) | Menetelmä nukleiinihappojen määrittämiseksi sekä menetelmässä käytettävä reagenssiyhdistelmä ja reagenssipakkaus | |
IT1303767B1 (it) | Metodo di quantificazione di acidi nucleici. | |
DE69122712D1 (de) | Verfahren zur nukleinsäure hybridisierung und amplifizierung | |
ES2165402T3 (es) | Procedimiento para la deteccion sensible de acidos nucleicos. | |
AU6146098A (en) | Method and apparatus for performing amplification of nucleic acid on supports | |
NL189427B (nl) | Werkwijze voor het identificeren van nucleinezuren. | |
EP0851940A4 (en) | METHOD FOR USING THE NUCLEOTIDE INTEGRASE TO CUT DNA AND INCLUDE NUCLEIC ACIDS | |
PT975800E (pt) | Doseamento de nucleotidos em solucao usando sondas anp | |
DE69637579D1 (de) | Methode zur Amplifizierung von Nukleinsäure und entsprechende Regenzien dafür | |
DK0587331T3 (da) | Nukleinsyreprober til Chlamydia pneumoniae. | |
NL195097C (nl) | Kwantificering van nucleinezuur moleculen en de daarbij gebruikte reagens set. | |
ATE227996T1 (de) | Zusammensetzung und verfahren zur regulierung von zellproliferation und zelltod | |
ES2083629T3 (es) | Inmovilizacion de acidos nucleicos. | |
DE59712415D1 (de) | Verfahren zur amplifikation von nukleinsäure | |
ES2149448T3 (es) | Agentes terapeuticos y analiticos. | |
DE69329824D1 (de) | Nachweis und amplifikation bestimmter nukleinsäuren mittels exonukleolytischer aktivität | |
ATE445021T1 (de) | Reduktion von kreuzkontaminationen bei nukleinsäureamplifikationen | |
DE3786978D1 (de) | Verfahren fuer die zufuehrung von kulturmedium und kultursystem. | |
ATE139118T1 (de) | Ribozymenhemmer | |
DE68920659D1 (de) | Nukleosidoxidase und analytisches verfahren. | |
FR2618160B1 (fr) | Technique enzymatique utilisant un chromogene lyophilise, trousse de reactif et procede de lyophilisation |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
Ref document number: 623682 Country of ref document: ES |