ES2136137T3 - Controles internos para reacciones de amplificacion isotermica de acidos nucleicos. - Google Patents

Controles internos para reacciones de amplificacion isotermica de acidos nucleicos.

Info

Publication number
ES2136137T3
ES2136137T3 ES94106632T ES94106632T ES2136137T3 ES 2136137 T3 ES2136137 T3 ES 2136137T3 ES 94106632 T ES94106632 T ES 94106632T ES 94106632 T ES94106632 T ES 94106632T ES 2136137 T3 ES2136137 T3 ES 2136137T3
Authority
ES
Spain
Prior art keywords
amplification reactions
nucleic acids
internal controls
isothermic amplification
isothermic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94106632T
Other languages
English (en)
Inventor
James G Nadeau
Michael C Little
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Application granted granted Critical
Publication of ES2136137T3 publication Critical patent/ES2136137T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

METODOS QUE EMPLEAN ESTANDARES INTERNOS DE OLIGONUCLEOTIDOS EN LAS REACCIONES ISOTERMICAS DE AMPLIFICACION DE ACIDO NUCLEICO PARA DETERMINAR LA EFICACIA DE LA REACCION DE AMPLIFICACION Y PARA CUANTIFICAR LOS NIVELES DE ACIERTO DE PREAMPLIFICACION.
ES94106632T 1993-05-05 1994-04-28 Controles internos para reacciones de amplificacion isotermica de acidos nucleicos. Expired - Lifetime ES2136137T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/058,648 US5457027A (en) 1993-05-05 1993-05-05 Internal controls for isothermal nucleic acid amplification reactions

Publications (1)

Publication Number Publication Date
ES2136137T3 true ES2136137T3 (es) 1999-11-16

Family

ID=22018080

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94106632T Expired - Lifetime ES2136137T3 (es) 1993-05-05 1994-04-28 Controles internos para reacciones de amplificacion isotermica de acidos nucleicos.

Country Status (8)

Country Link
US (2) US5457027A (es)
EP (1) EP0623682B1 (es)
JP (1) JP3127079B2 (es)
AU (1) AU675503B2 (es)
CA (1) CA2121658C (es)
DE (1) DE69420454T2 (es)
ES (1) ES2136137T3 (es)
SG (1) SG50707A1 (es)

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ZA936015B (en) * 1992-08-24 1994-03-10 Akzo Nv Elimination of false negatives in nuleic acid detection.
US5470723A (en) 1993-05-05 1995-11-28 Becton, Dickinson And Company Detection of mycobacteria by multiplex nucleic acid amplification
US5457027A (en) * 1993-05-05 1995-10-10 Becton, Dickinson And Company Internal controls for isothermal nucleic acid amplification reactions
AT401270B (de) * 1994-09-26 1996-07-25 Immuno Ag Verfahren zur quantifizierung von genomischer dna
US5705365A (en) * 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5882867A (en) * 1995-06-07 1999-03-16 Dade Behring Marburg Gmbh Detection of nucleic acids by formation of template-dependent product
JP3996639B2 (ja) * 1995-06-07 2007-10-24 ジェン−プローブ・インコーポレーテッド 生成物の増幅後レベルから核酸標的配列の増幅前レベルを決定するための方法とキット
US5710029A (en) * 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5736333A (en) * 1996-06-04 1998-04-07 The Perkin-Elmer Corporation Passive internal references for the detection of nucleic acid amplification products
US6558901B1 (en) * 1997-05-02 2003-05-06 Biomerieux Vitek Nucleic acid assays
IL124275A (en) * 1997-05-02 2002-03-10 Bio Merieux Vitek Inc A method to produce sequences of nucleic acids
US6289229B1 (en) 1998-01-20 2001-09-11 Scimed Life Systems, Inc. Readable probe array for in vivo use
US6365346B1 (en) * 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
US6066458A (en) * 1998-05-18 2000-05-23 Becton, Dickinson And Company Methods, apparatus and computer program products for determining quantities of nucleic acid sequences in samples using standard curves and amplification ratio estimates
US8012719B2 (en) 1998-11-17 2011-09-06 Paolo Lusso Method for the quantitative detection of nucleic acids
IT1303767B1 (it) 1998-11-17 2001-02-23 San Raffaele Centro Fond Metodo di quantificazione di acidi nucleici.
AU2410500A (en) 1999-01-11 2000-08-01 President And Fellows Of Harvard College Isothermal amplification of dna
US6235479B1 (en) 1999-04-13 2001-05-22 Bio Merieux, Inc. Methods and devices for performing analysis of a nucleic acid sample
EP1238100B1 (en) 1999-07-23 2005-09-07 Gen-Probe Incorporated Polynucleotide amplification method
US7271781B2 (en) * 2000-03-03 2007-09-18 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Multiplex hybridization system for identification of pathogenic mycobacterium and method of use
EP1236804A1 (en) * 2001-03-02 2002-09-04 Boehringer Mannheim Gmbh A method for determination of a nucleic acid using a control
ES2305144T3 (es) * 2001-03-02 2008-11-01 F. Hoffmann-La Roche Ag Metodo para la determinacion de un acido nucleico utilizando un control.
AU2002323213B2 (en) * 2001-08-16 2008-03-13 Stratagene California Compositions and methods comprising control nucleic acid
US7122314B2 (en) * 2002-01-30 2006-10-17 Id Biomedical Corporation Methods for detecting vancomycin-resistant microorganisms and compositions therefor
US20040024193A1 (en) * 2002-07-26 2004-02-05 Williams Gregg T. Method of detecting and quantifying hepatitis C virus
JP4603356B2 (ja) * 2002-07-26 2010-12-22 アボット・ラボラトリーズ Ｃ型肝炎ウイルスの検出及び定量方法
US7829312B2 (en) * 2003-04-11 2010-11-09 Dna Landmarks, Inc. Methods for relative quantification of specific nucleic acid sequences
CA2522753C (en) * 2003-04-18 2014-06-10 Becton, Dickinson And Company Immuno-amplification
WO2004097046A1 (en) * 2003-05-01 2004-11-11 Isis Innovation Limited Stable internal standard for pcr and rt- pcr reactions
US7229800B2 (en) * 2004-04-16 2007-06-12 Becton, Dickinson And Company Neisseria gonorrhoeae assay
AU2005291899A1 (en) 2004-09-30 2006-04-13 Gen-Probe Incorporated Assay for detecting and quantifying HIV-1
EP1861721B1 (en) 2005-03-10 2017-05-03 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
JP2010511390A (ja) 2006-11-30 2010-04-15 ジェン−プロウブ インコーポレイテッド 予め規定されたマスター検量線の調整を使用する定量方法
US20080274458A1 (en) * 2007-05-01 2008-11-06 Latham Gary J Nucleic acid quantitation methods
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
WO2010139767A1 (de) * 2009-06-04 2010-12-09 Qiagen Gmbh Amplifikation komplexer nukleinsäuren
US8563298B2 (en) 2010-10-22 2013-10-22 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
ES2576927T3 (es) 2010-10-22 2016-07-12 T2 Biosystems, Inc. Sistemas de RMN y métodos para la detección rápida de analitos
EP2794915B1 (en) 2011-12-22 2017-02-08 Centre National de la Recherche Scientifique (CNRS) Method of dna detection and quantification by single-molecule hybridization and manipulation
EP2877952B1 (en) 2012-07-27 2023-04-12 Gen-Probe Incorporated Dual reference calibration method and system for quantifying polynucleotides
US20140193819A1 (en) * 2012-10-31 2014-07-10 Becton, Dickinson And Company Methods and compositions for modulation of amplification efficiency
CN105264086B (zh) 2013-04-01 2018-06-15 贝克顿·迪金森公司 用于流感诊断的方法和试剂盒
KR20170099939A (ko) * 2014-12-16 2017-09-01 가반 인스티튜트 오브 메디컬 리서치 시퀀싱 컨트롤
CA3011991A1 (en) 2016-01-21 2017-07-27 T2 Biosystems, Inc. Rapid antimicrobial susceptibility testing using high-sensitivity direct detection methods
EP4151751A1 (en) 2016-04-14 2023-03-22 T2 Biosystems, Inc. Methods and systems for amplification in complex samples

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69011101T2 (de) * 1989-03-03 1995-01-26 Genentech Inc Methoden zur in vitro-dna-amplifikation, zum genomischen klonieren und zur genkartierung.
US5219727A (en) * 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
GB9016163D0 (en) * 1990-07-24 1990-09-05 Cemu Bioteknik Chemical method
WO1992011273A1 (en) * 1990-12-20 1992-07-09 F.Hoffmann-La Roche Ag Pcr primers for detection of legionella species and methods for controlling visual intensity in hybridization assays
GB9116042D0 (en) * 1991-07-24 1991-09-11 Royal Free Hosp School Med Diagnostic method
ATE257860T1 (de) * 1991-08-02 2004-01-15 Biomerieux Bv Quantifikation von nukleinsäuren
US5457027A (en) * 1993-05-05 1995-10-10 Becton, Dickinson And Company Internal controls for isothermal nucleic acid amplification reactions

Also Published As

Publication number Publication date
DE69420454D1 (de) 1999-10-14
US5457027A (en) 1995-10-10
AU6058094A (en) 1994-11-10
US5840487A (en) 1998-11-24
AU675503B2 (en) 1997-02-06
CA2121658C (en) 1996-12-03
JP3127079B2 (ja) 2001-01-22
JPH06343497A (ja) 1994-12-20
CA2121658A1 (en) 1994-11-06
DE69420454T2 (de) 1999-12-23
EP0623682A1 (en) 1994-11-09
SG50707A1 (en) 1998-07-20
EP0623682B1 (en) 1999-09-08

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