DE69122712D1 - Verfahren zur nukleinsäure hybridisierung und amplifizierung - Google Patents

Verfahren zur nukleinsäure hybridisierung und amplifizierung

Info

Publication number
DE69122712D1
DE69122712D1 DE69122712T DE69122712T DE69122712D1 DE 69122712 D1 DE69122712 D1 DE 69122712D1 DE 69122712 T DE69122712 T DE 69122712T DE 69122712 T DE69122712 T DE 69122712T DE 69122712 D1 DE69122712 D1 DE 69122712D1
Authority
DE
Germany
Prior art keywords
amplification
nucleic acid
acid hybridization
hybridization
nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69122712T
Other languages
English (en)
Other versions
DE69122712T2 (de
Inventor
David Zarling
Elissa Sena
Christopher Green
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Daikin Industries Ltd
Original Assignee
Daikin Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Daikin Industries Ltd filed Critical Daikin Industries Ltd
Application granted granted Critical
Publication of DE69122712D1 publication Critical patent/DE69122712D1/de
Publication of DE69122712T2 publication Critical patent/DE69122712T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69122712T 1990-05-07 1991-04-17 Verfahren zur nukleinsäure hybridisierung und amplifizierung Expired - Fee Related DE69122712T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/520,321 US5223414A (en) 1990-05-07 1990-05-07 Process for nucleic acid hybridization and amplification
PCT/US1991/002626 WO1991017267A1 (en) 1990-05-07 1991-04-17 Process for nucleic acid hybridization and amplification

Publications (2)

Publication Number Publication Date
DE69122712D1 true DE69122712D1 (de) 1996-11-21
DE69122712T2 DE69122712T2 (de) 1997-02-20

Family

ID=24072098

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69122712T Expired - Fee Related DE69122712T2 (de) 1990-05-07 1991-04-17 Verfahren zur nukleinsäure hybridisierung und amplifizierung

Country Status (6)

Country Link
US (1) US5223414A (de)
EP (1) EP0481065B1 (de)
JP (1) JP3010738B2 (de)
CA (1) CA2056983C (de)
DE (1) DE69122712T2 (de)
WO (1) WO1991017267A1 (de)

Families Citing this family (118)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE153707T1 (de) * 1990-05-07 1997-06-15 Daikin Ind Ltd Diagnostische anwendungen der bildung von doppelten d-loops.
US5510473A (en) * 1990-11-09 1996-04-23 The United States Of American As Represented By The Secretary Of Health And Human Services Cloning of the recA gene from thermus aquaticus YT-1
US5506098A (en) * 1991-09-04 1996-04-09 Daikin Industries, Ltd. In situ hybridization method
AU672969B2 (en) * 1992-03-10 1996-10-24 United States of America, as represented by The Secretary, Department of Health & Human Services, The Exchangeable template reaction
US20020108136A1 (en) * 1997-03-21 2002-08-08 Sri Transgenic animals produced by homologous sequence targeting
AU4115693A (en) * 1992-04-24 1993-11-29 Sri International In vivo homologous sequence targeting in eukaryotic cells
DE4216949C2 (de) * 1992-05-22 1997-07-24 Christoph Prof Dr Dr Cremer Nichtenzymatisches Verfahren zur In-situ-Hybridisierung bei spezifischen Proben
US5674992A (en) * 1992-08-28 1997-10-07 Cornell Research Foundation, Inc. CDNA encoding a recA homolog in eukaryotes
US5491086A (en) * 1993-05-14 1996-02-13 Hoffmann-La Roche Inc. Purified thermostable nucleic acid polymerase and DNA coding sequences from pyrodictium species
EP0687738A4 (de) * 1993-12-28 1999-05-19 Daikin Ind Ltd IN SITU HYBRIDISIERUNGSMETHODE UNTER NUTZUNG VON RecA PROTEIN, WELCHES ZUR VERWENDUNG IN DIESER METHODE EINEN MARKER ODER LIGANDEN HAT
AU687535B2 (en) 1994-03-16 1998-02-26 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
CA2263815A1 (en) 1996-08-29 1998-03-05 Koji Kigawa Methods for targeting, enriching, detecting and/or isolating target nucleic acid sequence using reca-like recombinase
US5948653A (en) * 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
US6489455B2 (en) 1997-05-21 2002-12-03 Clontech Laboratories, Inc. Methods of assaying differential expression
US5994076A (en) * 1997-05-21 1999-11-30 Clontech Laboratories, Inc. Methods of assaying differential expression
CA2365263A1 (en) * 1999-03-12 2000-09-21 Mcgill University A method for increasing the processivity of a dna- or rna-dependent polymerase and compositions therefor
WO2001062968A2 (en) * 2000-02-25 2001-08-30 General Atomics Mutant nucleic binding enzymes and use thereof in diagnostic, detection and purification methods
US20020132350A1 (en) * 2000-09-14 2002-09-19 Pioneer Hi-Bred International, Inc. Targeted genetic manipulation using Mu bacteriophage cleaved donor complex
CA2439098A1 (en) * 2001-02-21 2002-10-03 Gene Check, Inc. Mutation detection using muts and reca
EP1414840A4 (de) * 2001-03-27 2005-04-13 Univ Delaware Genomics-anwendungen für modifizierte oligonukleotide
ES2305289T3 (es) * 2001-04-20 2008-11-01 The Penn State Research Foundation Metodo de manipulacion de acidos nucleicos.
US7468244B2 (en) * 2001-09-27 2008-12-23 University Of Delaware Polymorphism detection and separation
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US7244562B2 (en) * 2001-11-01 2007-07-17 Gene Check, Inc. RecA assisted detection of mutations, single nucleotide polymorphisms and specific sequences
DE60322044D1 (de) * 2002-02-21 2008-08-21 Asm Scient Inc Rekombinase-polymerase-amplifikation
US7399590B2 (en) * 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
DE60324819D1 (de) * 2002-09-17 2009-01-02 Aisin Cosmos R & D Co Ltd Verfahren zur amplifikation von nukleinsäure, kit zur amplifikation von nukleinsäure, verfahren zum nachweis von einzelnukleotid-polymorphismus und reagentienkit zum nachweis von einzelnukleotid-polymorphismus
JP2004113108A (ja) * 2002-09-26 2004-04-15 Aisin Seiki Co Ltd Dna回収法
BRPI0508100A (pt) * 2004-02-28 2007-07-17 Chang Ning J Wang complexos de ácido nucléico bem como processo para detecção e sistema multifásico
EP3540073B1 (de) * 2004-06-01 2021-08-25 Abbott Diagnostics Scarborough, Inc. Rekombinase-polymerase-amplifikation
CA2569491A1 (en) 2004-06-02 2005-12-15 Asm Scientific, Inc. 2'-nitrobenzyl-modified ribonucleotides
US7700281B2 (en) * 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
JP4534138B2 (ja) * 2004-12-21 2010-09-01 アイシン精機株式会社 核酸増幅反応における高度好熱菌由来タンパク質の活性化方法およびその利用
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7435561B2 (en) 2005-07-25 2008-10-14 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
US20090061413A1 (en) * 2006-02-27 2009-03-05 Salinas Frank G Isothermal screening of hiv-1 related nucleic acids
US20070202522A1 (en) * 2006-02-27 2007-08-30 Salinas Frank G Isothermal screening of tumor cell related nucleic acids
US20080220421A1 (en) * 2006-02-27 2008-09-11 Salinas Frank G Isothermal screening for nucleic acids associated with diseases and conditions of the gi tract
US20080003600A1 (en) * 2006-02-27 2008-01-03 Salinas Frank G Isothermal screening of breast cancer related nucleic acid
EP2029782B1 (de) 2006-05-04 2014-11-26 Alere San Diego, Inc. Rekombinase-polymerase-verstärkung
US8951731B2 (en) * 2007-10-15 2015-02-10 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US20120156728A1 (en) 2010-12-17 2012-06-21 Life Technologies Corporation Clonal amplification of nucleic acid on solid surface with template walking
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9309566B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
WO2010135310A1 (en) 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates
WO2010141940A1 (en) 2009-06-05 2010-12-09 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
CA2826131C (en) 2011-02-02 2019-11-05 Jay Ashok Shendure Massively parallel continguity mapping
CA2831140C (en) 2011-04-07 2017-11-07 Alere San Diego, Inc. Monitoring recombinase polymerase amplification mixtures
EP3095879B1 (de) 2012-04-19 2018-09-19 Life Technologies Corporation Nukleinsäureamplifikation
CN109486902B (zh) 2012-04-19 2023-02-28 生命技术公司 核酸扩增
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
EP2895620B1 (de) 2012-09-11 2017-08-02 Life Technologies Corporation Nukleinsäureamplifikation
WO2014043143A1 (en) 2012-09-11 2014-03-20 Life Technologies Corporation Nucleic acid amplification
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
US10545161B2 (en) 2013-03-11 2020-01-28 Cue Health Inc. Systems and methods for detection and quantification of analytes
EP2861996B1 (de) 2013-03-11 2019-03-06 Cue Health Inc. Probenanalyse-kartusche
US9623409B2 (en) 2013-03-11 2017-04-18 Cue Inc. Cartridges, kits, and methods for enhanced mixing for detection and quantification of analytes
ES2724824T3 (es) 2013-03-13 2019-09-16 Illumina Inc Métodos para la secuenciación de ácidos nucleicos
CA2931533C (en) 2013-12-09 2023-08-08 Illumina, Inc. Methods and compositions for targeted nucleic acid sequencing
DK3083994T3 (da) 2013-12-20 2021-09-13 Illumina Inc Bevarelse af genomisk konnektivitetsinformation i fragmenterede genomiske DNA-prøver
WO2015113725A1 (en) 2014-02-03 2015-08-06 Thermo Fisher Scientific Baltics Uab Method for controlled dna fragmentation
USD745423S1 (en) 2014-05-12 2015-12-15 Cue Inc. Automated analyzer test cartridge and sample collection device for analyte detection
CA2952058A1 (en) 2014-06-13 2015-12-17 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
CN106574305B (zh) 2014-06-13 2021-03-02 生命技术公司 多重核酸扩增
SG11201610910QA (en) 2014-06-30 2017-01-27 Illumina Inc Methods and compositions using one-sided transposition
WO2016040602A1 (en) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv
AU2015323936B2 (en) 2014-09-29 2021-03-11 Illumina Cambridge Limited Recombinase mutants
JP6808617B2 (ja) 2014-10-17 2021-01-06 イルミナ ケンブリッジ リミテッド 連続性を維持した転位
MY182476A (en) 2014-11-11 2021-01-25 Illumina Cambridge Ltd Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
EP3882356A1 (de) 2014-12-15 2021-09-22 Illumina, Inc. Zusammensetzungen und verfahren zur einzelmolekülplatzierung auf einem substrat
ES2789349T3 (es) 2015-05-29 2020-10-26 Illumina Cambridge Ltd Utilización mejorada de cebadores de superficie en grupos
CN107849600A (zh) 2015-06-09 2018-03-27 生命技术公司 用于分子标记的方法、系统、组合物、试剂盒、装置和计算机可读媒体
EP3689466B1 (de) 2015-07-17 2024-06-05 Cue Health Inc. System zur erweiterten detektion und quantifizierung von analyten
US20180363037A1 (en) 2015-12-09 2018-12-20 Life Technologies Corporation Detection and quantification of nucleic acid molecules associated with a surface
EP3402880B1 (de) 2016-01-15 2024-01-03 Thermo Fisher Scientific Baltics UAB Thermophile dna-polymerase-mutanten
CN112892622B (zh) 2016-03-28 2022-08-30 亿明达股份有限公司 多平面微阵列
SG11201807056YA (en) 2016-03-28 2018-10-30 Illumina Inc Recombinase mutants
CN109312396A (zh) 2016-04-07 2019-02-05 伊鲁米那股份有限公司 用于构建标准化核酸文库的方法和系统
EP3469078B1 (de) 2016-06-10 2024-03-27 Life Technologies Corporation Verfahren und zusammensetzungen zur erweiterung von nukleinsäuren
US11535883B2 (en) 2016-07-22 2022-12-27 Illumina, Inc. Single cell whole genome libraries and combinatorial indexing methods of making thereof
WO2018071522A1 (en) 2016-10-11 2018-04-19 Life Technologies Corporation Rapid amplification of nucleic acids
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
WO2018140540A1 (en) 2017-01-25 2018-08-02 Cue Health Inc. Systems and methods for enhanced detection and quantification of analytes
FI3842545T3 (fi) 2017-04-23 2023-01-31 Koostumuksia ja menetelmiä näytteiden tunnistuksen parantamiseksi indeksoiduissa nukleiinihappokirjastoissa
CA3059839C (en) 2017-04-23 2023-01-03 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
EP3872187B1 (de) 2017-04-23 2022-09-21 Illumina Cambridge Limited Zusammensetzungen und verfahren zur verbesserung der probenidentifikation in indizierten nukleinsäurebibliotheken
ES2959047T3 (es) 2017-06-07 2024-02-19 Univ Oregon Health & Science Bibliotecas de genoma completo de célula individual para la secuenciación de metilación
WO2018231818A1 (en) 2017-06-16 2018-12-20 Life Technologies Corporation Control nucleic acids, and compositions, kits, and uses thereof
WO2019002178A1 (en) 2017-06-26 2019-01-03 Thermo Fisher Scientific Baltics Uab DNA MUTANTS THERMOPHILIC POLYMERASES
CN110785813A (zh) 2017-07-31 2020-02-11 伊鲁米那股份有限公司 具有多路生物样本聚合的测序系统
US11293056B2 (en) 2017-11-07 2022-04-05 Life Technologies Corporation Methods and compositions for manipulating nucleic acids
CA3064622A1 (en) 2018-04-02 2019-10-10 Illumina, Inc. Compositions and methods for making controls for sequence-based genetic testing
CA3067435C (en) 2018-05-17 2023-09-12 Illumina, Inc. High-throughput single-cell sequencing with reduced amplification bias
SG11202000905PA (en) 2018-06-04 2020-02-27 Illumina Inc High-throughput single-cell transcriptome libraries and methods of making and of using
FI3899037T3 (fi) 2018-12-19 2023-11-21 Illumina Inc Menetelmiä polynukleotidiklusterin klonaalisuusprioriteetin parantamiseksi
KR20210134598A (ko) 2019-03-01 2021-11-10 일루미나, 인코포레이티드 고-처리량 단일-핵 및 단일-세포 라이브러리 및 이의 제조 및 사용 방법
EP3963103B1 (de) 2019-05-03 2024-02-28 Life Technologies Corporation Verfahren und zusammensetzungen zur manipulation von nukleinsäuren
US11905558B2 (en) 2019-08-21 2024-02-20 Life Technologies Corporation System and method for sequencing
CA3134746A1 (en) 2019-12-19 2021-06-24 Illumina, Inc. High-throughput single-cell libraries and methods of making and of using
AU2021249046A1 (en) 2020-03-30 2022-10-20 Illumina, Inc. Methods and compositions for preparing nucleic acid libraries
CA3177286A1 (en) 2020-05-12 2021-11-18 Illumina Inc. Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
WO2022006081A1 (en) 2020-06-30 2022-01-06 Illumina, Inc. Catalytically controlled sequencing by synthesis to produce scarless dna
US20220170093A1 (en) 2020-11-16 2022-06-02 Life Technologies Corporation System and method for sequencing
AU2022212112A1 (en) 2021-01-29 2023-08-17 Illumina Cambridge Limited Methods, compositions and kits to improve seeding efficiency of flow cells with polynucleotides
US20220333178A1 (en) 2021-03-22 2022-10-20 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
CA3223722A1 (en) 2022-04-07 2023-10-12 Illumina, Inc. Altered cytidine deaminases and methods of use
NL2033124B1 (en) 2022-09-23 2024-03-29 Rapidemic B V Methods and device for multiple-label nucleic acid amplification and detection
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
WO2024069581A1 (en) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Helicase-cytidine deaminase complexes and methods of use
US20240209419A1 (en) 2022-12-14 2024-06-27 Illumina, Inc. Systems and Methods for Capture and Enrichment of Clustered Beads on Flow Cell Substrates

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4713337A (en) * 1985-01-03 1987-12-15 Massachusetts Institute Of Technology Method for deletion of a gene from a bacteria
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
AU606043B2 (en) 1985-03-28 1991-01-31 F. Hoffmann-La Roche Ag Detection of viruses by amplification and hybridization
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4888274A (en) * 1985-09-18 1989-12-19 Yale University RecA nucleoprotein filament and methods
JPS63109800A (ja) 1986-10-27 1988-05-14 Rikagaku Kenkyusho Dna塩基配列決定法
EP0322311B1 (de) 1987-12-21 1994-03-23 Applied Biosystems, Inc. Verfahren und Testsatz zum Nachweis einer Nukleinsäure-Sequenz

Also Published As

Publication number Publication date
EP0481065A1 (de) 1992-04-22
DE69122712T2 (de) 1997-02-20
WO1991017267A1 (en) 1991-11-14
EP0481065B1 (de) 1996-10-16
JP3010738B2 (ja) 2000-02-21
CA2056983A1 (en) 1991-11-08
JPH04507198A (ja) 1992-12-17
CA2056983C (en) 2003-07-01
US5223414A (en) 1993-06-29

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