ES2156861T3 - Secuenciacion ciclica de dna. - Google Patents

Secuenciacion ciclica de dna.

Info

Publication number
ES2156861T3
ES2156861T3 ES92920100T ES92920100T ES2156861T3 ES 2156861 T3 ES2156861 T3 ES 2156861T3 ES 92920100 T ES92920100 T ES 92920100T ES 92920100 T ES92920100 T ES 92920100T ES 2156861 T3 ES2156861 T3 ES 2156861T3
Authority
ES
Spain
Prior art keywords
dna
cyclical
sequencing
cyclical sequencing
preparing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92920100T
Other languages
English (en)
Inventor
Carl W Fuller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions USA LLC
Original Assignee
Amersham Pharmacia Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Pharmacia Biotech Inc filed Critical Amersham Pharmacia Biotech Inc
Application granted granted Critical
Publication of ES2156861T3 publication Critical patent/ES2156861T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

SE PRESENTA UN METODO PARA LA SECUENCIACION DE DNA QUE INCLUYE LOS SIGUIENTES PASOS: EL SUMINISTRO DE UN INICIADOR DE POLINUCLEOTIDO COMPLEMENTARIO CON UNA REGION DE DNA, EL SUMINISTRO DEL DNA A SECUENCIAR, Y LA PUESTA EN CONTACTO DE ESE INICIADOR Y EL DNA ENTRE SI EN LA PRESENCIA DE UNA POLIMERASA DE DNA Y ENTRE 1 Y 3 DNTP, ESTANDO AL MENOS UNO DE LOS DNTP ETIQUETADO. EL INICIADOR Y EL DNA SE PONEN EN CONTACTO BAJO CONDICIONES QUE PERMITAN LA EXTENSION DEL INICIADOR MEDIANTE LA ADICION DE UNO O MAS DNTPS AL INICIADOR PARA FORMAR UN INICIADOR EXTENDIDO. EL INICIADOR Y EL DNA SE DISOCIAN ENTONCES, GENERALMENTE MEDIANTE CALENTAMIENTO, Y LOS PASOS DE PUESTA EN CONTACTO Y DISOCIACION SE REPITEN UNA PLURALIDAD DE VECES (HABITUALMENTE ENTRE 10 Y 200 VECES). FINALMENTE, EL INICIADOR EXTENDIDO SE PONE EN CONTACTO CON EL DNA EN LA PRESENCIA DE UNA POLIMERASA DE DNA (QUE ES GENERALMENTE LA MISMA POLIMERASA QUE SE USO EN EL PASO DE ETIQUETADO INICIAL) LOS CUATRO DNTPS Y EL AGENTE FINALIZADOR DE LA CADENA.
ES92920100T 1991-09-27 1992-08-28 Secuenciacion ciclica de dna. Expired - Lifetime ES2156861T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/767,137 US5674679A (en) 1991-09-27 1991-09-27 DNA cycle sequencing

Publications (1)

Publication Number Publication Date
ES2156861T3 true ES2156861T3 (es) 2001-08-01

Family

ID=25078586

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92920100T Expired - Lifetime ES2156861T3 (es) 1991-09-27 1992-08-28 Secuenciacion ciclica de dna.

Country Status (9)

Country Link
US (3) US5674679A (es)
EP (2) EP1038973B1 (es)
JP (1) JP2695045B2 (es)
AT (2) ATE198913T1 (es)
AU (1) AU2573992A (es)
CA (1) CA2119140A1 (es)
DE (2) DE69233041T2 (es)
ES (1) ES2156861T3 (es)
WO (1) WO1993006243A1 (es)

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Also Published As

Publication number Publication date
WO1993006243A1 (en) 1993-04-01
DE69231664T2 (de) 2001-05-03
US5741640A (en) 1998-04-21
JP2695045B2 (ja) 1997-12-24
EP0654091A1 (en) 1995-05-24
DE69233041T2 (de) 2004-03-11
EP1038973A1 (en) 2000-09-27
EP0654091A4 (en) 1997-11-12
EP0654091B1 (en) 2001-01-24
EP1038973B1 (en) 2003-05-02
CA2119140A1 (en) 1993-04-01
DE69233041D1 (de) 2003-06-05
DE69231664D1 (de) 2001-03-01
US5674679A (en) 1997-10-07
JPH06510433A (ja) 1994-11-24
US5741676A (en) 1998-04-21
ATE198913T1 (de) 2001-02-15
ATE239094T1 (de) 2003-05-15
AU2573992A (en) 1993-04-27

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