ATE239094T1 - Behandlung von amplifizierter dns mit alkalischer phosphatase - Google Patents

Behandlung von amplifizierter dns mit alkalischer phosphatase

Info

Publication number
ATE239094T1
ATE239094T1 AT00111087T AT00111087T ATE239094T1 AT E239094 T1 ATE239094 T1 AT E239094T1 AT 00111087 T AT00111087 T AT 00111087T AT 00111087 T AT00111087 T AT 00111087T AT E239094 T1 ATE239094 T1 AT E239094T1
Authority
AT
Austria
Prior art keywords
alkaline phosphatase
treatment
amplified dna
dna
amplified
Prior art date
Application number
AT00111087T
Other languages
English (en)
Inventor
Carl W Fuller
Original Assignee
Amersham Biosciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Corp filed Critical Amersham Biosciences Corp
Application granted granted Critical
Publication of ATE239094T1 publication Critical patent/ATE239094T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT00111087T 1991-09-27 1992-08-28 Behandlung von amplifizierter dns mit alkalischer phosphatase ATE239094T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/767,137 US5674679A (en) 1991-09-27 1991-09-27 DNA cycle sequencing

Publications (1)

Publication Number Publication Date
ATE239094T1 true ATE239094T1 (de) 2003-05-15

Family

ID=25078586

Family Applications (2)

Application Number Title Priority Date Filing Date
AT00111087T ATE239094T1 (de) 1991-09-27 1992-08-28 Behandlung von amplifizierter dns mit alkalischer phosphatase
AT92920100T ATE198913T1 (de) 1991-09-27 1992-08-28 Zyklische dna sequenzierung

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT92920100T ATE198913T1 (de) 1991-09-27 1992-08-28 Zyklische dna sequenzierung

Country Status (9)

Country Link
US (3) US5674679A (de)
EP (2) EP0654091B1 (de)
JP (1) JP2695045B2 (de)
AT (2) ATE239094T1 (de)
AU (1) AU2573992A (de)
CA (1) CA2119140A1 (de)
DE (2) DE69233041T2 (de)
ES (1) ES2156861T3 (de)
WO (1) WO1993006243A1 (de)

Families Citing this family (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4214112A1 (de) * 1991-08-02 1993-02-04 Europ Lab Molekularbiolog Neues verfahren zur sequenzierung von nukleinsaeuren
EP0749497B1 (de) * 1994-03-08 2000-09-06 Amersham Pharmacia Biotech UK Limited Modifizierung von nukleotidanalogen
DE19515552A1 (de) * 1995-04-27 1996-10-31 Europ Lab Molekularbiolog Simultane Sequenzierung von Nukleinsäuren
US5928906A (en) * 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
IL119342A0 (en) * 1996-10-02 1997-01-10 Yeda Research And Development C Methods for priming and DNA sequencing
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
US6235471B1 (en) 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6197510B1 (en) 1998-10-01 2001-03-06 Bio-Id Diagnostic Inc. Multi-loci genomic analysis
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
AU1239901A (en) 1999-10-29 2001-05-14 Stratagene Compositions and methods utilizing dna polymerases
US20030228616A1 (en) * 1999-10-29 2003-12-11 Stratagene DNA polymerase mutants with reverse transcriptase activity
US20080199935A1 (en) * 1999-10-29 2008-08-21 Stratagene California Compositions and methods utilizing DNA polymerases
US20040009486A1 (en) * 1999-10-29 2004-01-15 Sorge Joseph A. Compositions and methods utilizing DNA polymerases
US8268605B2 (en) * 1999-10-29 2012-09-18 Agilent Technologies, Inc. Compositions and methods utilizing DNA polymerases
US20050123940A1 (en) * 1999-10-29 2005-06-09 Stratagene California Compositions and methods for synthesizing cDNA
US6762018B1 (en) * 1999-12-23 2004-07-13 Tetragen Sa Analysis of nucleotide polymorphisms at a site
US6379940B2 (en) 2000-03-21 2002-04-30 Usb Corporation Stable composition comprising a nuclease and a phosphatase
EP2803724A1 (de) * 2000-03-21 2014-11-19 Affymetrix, Inc. Stabile Zusammensetzung umfassend eine Nuklease und Phosphatase
US6994963B1 (en) * 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
US6379932B1 (en) * 2000-07-17 2002-04-30 Incyte Genomics, Inc. Single primer PCR amplification of RNA
GB0024827D0 (en) * 2000-10-10 2000-11-22 Norwegian Inst Of Fisheries & Product
EP1368497A4 (de) * 2001-03-12 2007-08-15 California Inst Of Techn Verfahren und vorrichtung zur analyse von polynukleotidsequenzen durch asynchrone basenverlängerung
KR20040068122A (ko) 2001-10-15 2004-07-30 바이오어레이 솔루션스 리미티드 공동 검색과 효소-매개된 탐지에 의한 다형성 좌위의 다중분석
WO2003068991A1 (en) * 2002-02-12 2003-08-21 Applera Corporation Polymerase compositions
US20040241723A1 (en) * 2002-03-18 2004-12-02 Marquess Foley Leigh Shaw Systems and methods for improving protein and milk production of dairy herds
CA2510166A1 (en) 2002-12-20 2004-09-30 Caliper Life Sciences, Inc. Single molecule amplification and detection of dna
GB2398383B (en) * 2003-02-12 2005-03-09 Global Genomics Ab Method and means for nucleic acid sequencing
WO2004072294A2 (en) * 2003-02-12 2004-08-26 Genizon Svenska Ab Methods and means for nucleic acid sequencing
EP2390352A1 (de) 2003-03-18 2011-11-30 Quantum Genetics Ireland Limited Systeme und Methoden zur Erhöhung der Milchproduktion bei Tieren
US7255994B2 (en) * 2003-06-10 2007-08-14 Applera Corporation Ligation assay
EP1704246A4 (de) * 2003-09-30 2007-11-28 Perkinelmer Las Inc Zusammensetzungen und verfahren zur genotypisierung von einzelnukleotidpolymorphismen
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
MXPA06009452A (es) 2004-02-19 2007-03-15 Univ Alberta Polimorfismos del promotor de leptin y sus usos.
DE602005020421D1 (de) 2004-02-19 2010-05-20 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
US7635562B2 (en) * 2004-05-25 2009-12-22 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
FI20040723A0 (fi) * 2004-05-26 2004-05-26 Orpana Aarne Menetelmä mRNA:n ilmentymistasojen kvantitatiiviseksi ja/tai suhteelliseksi mittaamiseksi pienissä biologisissa näytteissä
US7264934B2 (en) * 2004-06-10 2007-09-04 Ge Healthcare Bio-Sciences Corp. Rapid parallel nucleic acid analysis
WO2006039005A2 (en) * 2004-08-24 2006-04-13 New England Biolabs, Inc. Compositions and methods for accomplishing nucleotide depletion
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7604940B1 (en) * 2005-03-16 2009-10-20 Applied Biosystems, Llc Compositions and methods for analyzing isolated polynucleotides
US8785130B2 (en) 2005-07-07 2014-07-22 Bio-Id Diagnostic Inc. Use of markers including nucleotide sequence based codes to monitor methods of detection and identification of genetic material
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US20090305248A1 (en) * 2005-12-15 2009-12-10 Lander Eric G Methods for increasing accuracy of nucleic acid sequencing
US20080309926A1 (en) * 2006-03-08 2008-12-18 Aaron Weber Systems and methods for reducing detected intensity non uniformity in a laser beam
US7397546B2 (en) * 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US9150906B2 (en) 2006-06-28 2015-10-06 Bio-Id Diagnostic Inc. Determination of variants produced upon replication or transcription of nucleic acid sequences
US7501254B2 (en) * 2006-07-20 2009-03-10 Ghc Technologies, Inc. Methods and compositions for amplification and capture of nucleic acid sequences
US20090137405A1 (en) * 2007-11-16 2009-05-28 Christopher Bowman Detection of nucleic acid biomarkers using polymerization-based amplification
WO2009100080A1 (en) * 2008-02-04 2009-08-13 Life Technologies Corporation Composition and method for sequencing nucleic acid
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
GB2483810B (en) 2008-11-07 2012-09-05 Sequenta Inc Methods for correlating clonotypes with diseases in a population
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US8129168B2 (en) 2009-01-14 2012-03-06 Affymetrix, Inc. Recombinant colwellia psychrerythraea alkaline phosphatase and uses thereof
PT2387627E (pt) 2009-01-15 2016-06-03 Adaptive Biotechnologies Corp Determinação do perfil de imunidade adaptativa e métodos de geração de anticorpos monoclonais
WO2010151416A1 (en) 2009-06-25 2010-12-29 Fred Hutchinson Cancer Research Center Method of measuring adaptive immunity
US20120135414A1 (en) 2010-10-28 2012-05-31 Life Technologies Corporation Chemically-enhanced primer compositions, methods and kits
EP2788509B1 (de) 2011-12-09 2018-07-11 Adaptive Biotechnologies Corporation Diagnose von lymphoid-malignität und nachweis minimaler resterkrankungen
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
EP3372694A1 (de) 2012-03-05 2018-09-12 Adaptive Biotechnologies Corporation Festlegung von gepaarten immunrezeptorketten aus frequenzabgestimmten untereinheiten
SG10201507700VA (en) 2012-05-08 2015-10-29 Adaptive Biotechnologies Corp Compositions and method for measuring and calibrating amplification bias in multiplexed pcr reactions
CN105189779B (zh) 2012-10-01 2018-05-11 适应生物技术公司 通过适应性免疫受体多样性和克隆性表征进行的免疫能力评估
WO2015160439A2 (en) 2014-04-17 2015-10-22 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
WO2014145706A2 (en) 2013-03-15 2014-09-18 Life Technologies Corporation A recombinant fusion protein possessing nuclease and phosphatase activity
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
EP3114240B1 (de) 2014-03-05 2019-07-24 Adaptive Biotechnologies Corporation Verfahren unter verwendung von randomerhaltigen synthetischen molekülen
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
GB201414745D0 (en) 2014-08-19 2014-10-01 Articzymes As Exonucleases
US20170247756A1 (en) 2014-10-03 2017-08-31 Life Technologies Corporation Genetic sequence verification compositions, methods and kits
WO2016069886A1 (en) 2014-10-29 2016-05-06 Adaptive Biotechnologies Corporation Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
EP3498866A1 (de) 2014-11-25 2019-06-19 Adaptive Biotechnologies Corp. Charakterisierung von adaptiver immunreaktion auf impfungen oder infektionen unter verwendung von immunrepertoiresequenzierung
US10302494B2 (en) 2014-12-18 2019-05-28 Palo Alto Research Center Incorporated Obtaining spectral information from a moving object
US10048192B2 (en) * 2014-12-18 2018-08-14 Palo Alto Research Center Incorporated Obtaining spectral information from moving objects
AU2016222788B2 (en) 2015-02-24 2022-03-31 Adaptive Biotechnologies Corp. Methods for diagnosing infectious disease and determining HLA status using immune repertoire sequencing
AU2016242967B2 (en) 2015-04-01 2021-07-01 Adaptive Biotechnologies Corp. Method of identifying human compatible T cell receptors specific for an antigenic target
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4851331A (en) * 1986-05-16 1989-07-25 Allied Corporation Method and kit for polynucleotide assay including primer-dependant DNA polymerase
US4795699A (en) * 1987-01-14 1989-01-03 President And Fellows Of Harvard College T7 DNA polymerase
US5075216A (en) * 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
DE3923895A1 (de) * 1989-07-19 1991-01-24 Basf Ag Verfahren zur sequenzierung von desoxyribonukleinsaeuren
JP3087907B2 (ja) * 1990-02-16 2000-09-18 エフ．ホフマン ― ラ ロシュ アーゲー ポリメラーゼチェイン反応の特異性と簡便性の改良

Also Published As

Publication number Publication date
JPH06510433A (ja) 1994-11-24
WO1993006243A1 (en) 1993-04-01
US5674679A (en) 1997-10-07
JP2695045B2 (ja) 1997-12-24
DE69233041T2 (de) 2004-03-11
DE69231664D1 (de) 2001-03-01
EP0654091B1 (de) 2001-01-24
EP1038973B1 (de) 2003-05-02
EP0654091A4 (de) 1997-11-12
DE69233041D1 (de) 2003-06-05
DE69231664T2 (de) 2001-05-03
EP1038973A1 (de) 2000-09-27
US5741640A (en) 1998-04-21
EP0654091A1 (de) 1995-05-24
ATE198913T1 (de) 2001-02-15
US5741676A (en) 1998-04-21
AU2573992A (en) 1993-04-27
CA2119140A1 (en) 1993-04-01
ES2156861T3 (es) 2001-08-01

Similar Documents

Publication Publication Date Title
DE69233041D1 (de) Behandlung von amplifizierter DNS mit alkalischer Phosphatase
DE59007374D1 (de) Optisch gegengekoppelter Verstärker.
DE3874974T2 (de) Cmos-leistungsoperationsverstaerker.
DE3878069D1 (de) Breitbandverstaerker.
DE3864809D1 (de) Vorrichtung zum transport von vorgarnspulen.
DE3879600D1 (de) Operationsverstaerker.
MA21139A1 (fr) Methode herbicide utilisant le diflufenican .
DE3768847D1 (de) Offset-korrigierter verstaerker.
DE3850923D1 (de) Operationsverstärkerstufen.
ES2058444T3 (es) Aldehidos alifaticos aromaticos.
DK192890D0 (da) 24r-scumnol, fremgangsmaade til fremstilling heraf samt anvendelse af samme
DE287063T1 (de) Schaltung zum automatischen regeln des verstaerkung-bandbreite-produktes von operationsverstaerkern.
DE3862356D1 (de) Bremskraftverstaerker.
FI880320A0 (fi) Foerfarande foer framstaellning av n,n-(dibensohexatrienylen) urinaemnen.
AU7879487A (en) Proteine protease inhibitors from streptomyces
DE3851515T2 (de) Metalldampf-Laser-Apparat.
DE3763953D1 (de) Mechanischer kupplungsverstaerker.
DE69003908T2 (de) Verstärker mit hohem Wirkungsgrad.
DE3885846T2 (de) Kontrollierte Stromquelle.
DE69114949T2 (de) Gegentakt-Leistungsverstärker.
ATE52186T1 (de) Aldose-reductase-hemmer, nuetzlich bei der behandlung von glaukom.
DE3464599D1 (de) Alpha-allenic-alpha-amino acids
SE8704332L (sv) Drivhus for kultivering
NO913791D0 (no) Fremgangsmaate for nukleinsyrepaavisning ved binaer amplifikasjon.
IT8721941A0 (it) Procedimento di stampa su legno.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties