ATE239094T1 - Behandlung von amplifizierter dns mit alkalischer phosphatase - Google Patents

Behandlung von amplifizierter dns mit alkalischer phosphatase

Info

Publication number
ATE239094T1
ATE239094T1 AT00111087T AT00111087T ATE239094T1 AT E239094 T1 ATE239094 T1 AT E239094T1 AT 00111087 T AT00111087 T AT 00111087T AT 00111087 T AT00111087 T AT 00111087T AT E239094 T1 ATE239094 T1 AT E239094T1
Authority
AT
Austria
Prior art keywords
alkaline phosphatase
treatment
amplified dna
dna
amplified
Prior art date
Application number
AT00111087T
Other languages
English (en)
Inventor
Carl W Fuller
Original Assignee
Amersham Biosciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Corp filed Critical Amersham Biosciences Corp
Application granted granted Critical
Publication of ATE239094T1 publication Critical patent/ATE239094T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
AT00111087T 1991-09-27 1992-08-28 Behandlung von amplifizierter dns mit alkalischer phosphatase ATE239094T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/767,137 US5674679A (en) 1991-09-27 1991-09-27 DNA cycle sequencing

Publications (1)

Publication Number Publication Date
ATE239094T1 true ATE239094T1 (de) 2003-05-15

Family

ID=25078586

Family Applications (2)

Application Number Title Priority Date Filing Date
AT00111087T ATE239094T1 (de) 1991-09-27 1992-08-28 Behandlung von amplifizierter dns mit alkalischer phosphatase
AT92920100T ATE198913T1 (de) 1991-09-27 1992-08-28 Zyklische dna sequenzierung

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT92920100T ATE198913T1 (de) 1991-09-27 1992-08-28 Zyklische dna sequenzierung

Country Status (9)

Country Link
US (3) US5674679A (de)
EP (2) EP1038973B1 (de)
JP (1) JP2695045B2 (de)
AT (2) ATE239094T1 (de)
AU (1) AU2573992A (de)
CA (1) CA2119140A1 (de)
DE (2) DE69233041T2 (de)
ES (1) ES2156861T3 (de)
WO (1) WO1993006243A1 (de)

Families Citing this family (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4214112A1 (de) * 1991-08-02 1993-02-04 Europ Lab Molekularbiolog Neues verfahren zur sequenzierung von nukleinsaeuren
WO1995024505A1 (en) * 1994-03-08 1995-09-14 Amersham International Plc Modifying nucleotide analogues
DE19515552A1 (de) * 1995-04-27 1996-10-31 Europ Lab Molekularbiolog Simultane Sequenzierung von Nukleinsäuren
US5928906A (en) * 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
IL119342A0 (en) * 1996-10-02 1997-01-10 Yeda Research And Development C Methods for priming and DNA sequencing
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6235471B1 (en) 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6197510B1 (en) 1998-10-01 2001-03-06 Bio-Id Diagnostic Inc. Multi-loci genomic analysis
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US20040009486A1 (en) * 1999-10-29 2004-01-15 Sorge Joseph A. Compositions and methods utilizing DNA polymerases
US20030228616A1 (en) * 1999-10-29 2003-12-11 Stratagene DNA polymerase mutants with reverse transcriptase activity
US20080199935A1 (en) * 1999-10-29 2008-08-21 Stratagene California Compositions and methods utilizing DNA polymerases
US8268605B2 (en) * 1999-10-29 2012-09-18 Agilent Technologies, Inc. Compositions and methods utilizing DNA polymerases
US6946273B1 (en) 1999-10-29 2005-09-20 Stratagene California Compositions and methods utilizing DNA polymerases
US20050123940A1 (en) * 1999-10-29 2005-06-09 Stratagene California Compositions and methods for synthesizing cDNA
US6762018B1 (en) * 1999-12-23 2004-07-13 Tetragen Sa Analysis of nucleotide polymorphisms at a site
WO2001070943A2 (en) * 2000-03-21 2001-09-27 Usb Corporation Stable composition comprising a nuclease and a phosphatase
US6379940B2 (en) 2000-03-21 2002-04-30 Usb Corporation Stable composition comprising a nuclease and a phosphatase
US6994963B1 (en) * 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
US6379932B1 (en) * 2000-07-17 2002-04-30 Incyte Genomics, Inc. Single primer PCR amplification of RNA
GB0024827D0 (en) * 2000-10-10 2000-11-22 Norwegian Inst Of Fisheries & Product
CA2440754A1 (en) * 2001-03-12 2002-09-19 Stephen Quake Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
ES2661167T3 (es) * 2001-10-15 2018-03-27 Bioarray Solutions Ltd. Análisis multiplexado de loci polimórficos mediante consulta simultánea y detección mediada por enzimas
JP2005517413A (ja) * 2002-02-12 2005-06-16 アプレラ コーポレイション ポリメラーゼ組成物
US20040241723A1 (en) * 2002-03-18 2004-12-02 Marquess Foley Leigh Shaw Systems and methods for improving protein and milk production of dairy herds
JP4395133B2 (ja) * 2002-12-20 2010-01-06 カリパー・ライフ・サイエンシズ・インク. Dnaの単一分子増幅および検出
GB2398383B (en) * 2003-02-12 2005-03-09 Global Genomics Ab Method and means for nucleic acid sequencing
JP2006517798A (ja) * 2003-02-12 2006-08-03 イェニソン スベンスカ アクティエボラーグ 核酸配列用の方法及び手段
EP2390352A1 (de) 2003-03-18 2011-11-30 Quantum Genetics Ireland Limited Systeme und Methoden zur Erhöhung der Milchproduktion bei Tieren
WO2005047543A2 (en) * 2003-06-10 2005-05-26 Applera Corporation Ligation assay
WO2005033328A2 (en) * 2003-09-30 2005-04-14 Perkinelmer Las, Inc. Compositions and processes for genotyping single nucleotide polymorphisms
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20050202484A1 (en) 2004-02-19 2005-09-15 The Governors Of The University Of Alberta Leptin promoter polymorphisms and uses thereof
EP1716254B1 (de) 2004-02-19 2010-04-07 Helicos Biosciences Corporation Verfahren zur analyse von polynukleotidsequenzen
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
EP1766090B1 (de) * 2004-05-25 2011-04-27 Helicos Biosciences Corporation Verfahren zur nukleinsäureimmobilisierung
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
FI20040723A0 (fi) * 2004-05-26 2004-05-26 Orpana Aarne Menetelmä mRNA:n ilmentymistasojen kvantitatiiviseksi ja/tai suhteelliseksi mittaamiseksi pienissä biologisissa näytteissä
US7264934B2 (en) * 2004-06-10 2007-09-04 Ge Healthcare Bio-Sciences Corp. Rapid parallel nucleic acid analysis
US20060292584A1 (en) * 2004-08-24 2006-12-28 New England Biolabs, Inc. Compositions and methods for accomplishing nucleotide depletion
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7604940B1 (en) * 2005-03-16 2009-10-20 Applied Biosystems, Llc Compositions and methods for analyzing isolated polynucleotides
US8785130B2 (en) 2005-07-07 2014-07-22 Bio-Id Diagnostic Inc. Use of markers including nucleotide sequence based codes to monitor methods of detection and identification of genetic material
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
WO2007070642A2 (en) * 2005-12-15 2007-06-21 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
US7397546B2 (en) * 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US20080309926A1 (en) * 2006-03-08 2008-12-18 Aaron Weber Systems and methods for reducing detected intensity non uniformity in a laser beam
US9150906B2 (en) 2006-06-28 2015-10-06 Bio-Id Diagnostic Inc. Determination of variants produced upon replication or transcription of nucleic acid sequences
US7501254B2 (en) * 2006-07-20 2009-03-10 Ghc Technologies, Inc. Methods and compositions for amplification and capture of nucleic acid sequences
US20090137405A1 (en) * 2007-11-16 2009-05-28 Christopher Bowman Detection of nucleic acid biomarkers using polymerization-based amplification
WO2009100080A1 (en) * 2008-02-04 2009-08-13 Life Technologies Corporation Composition and method for sequencing nucleic acid
US8691510B2 (en) 2008-11-07 2014-04-08 Sequenta, Inc. Sequence analysis of complex amplicons
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
WO2010053587A2 (en) 2008-11-07 2010-05-14 Mlc Dx Incorporated Methods of monitoring conditions by sequence analysis
US8129168B2 (en) * 2009-01-14 2012-03-06 Affymetrix, Inc. Recombinant colwellia psychrerythraea alkaline phosphatase and uses thereof
PL2387627T3 (pl) 2009-01-15 2016-10-31 Profilowanie odporności adaptacyjnej i wytwarzanie przeciwciał monoklonalnych
KR20120044941A (ko) 2009-06-25 2012-05-08 프레드 헛친슨 켄서 리서치 센터 적응 면역의 측정방법
WO2012058647A1 (en) 2010-10-28 2012-05-03 Life Technologies Corporation Chemically-enhanced primer compositions, methods and kits
EP3904536B1 (de) 2011-12-09 2025-10-29 Adaptive Biotechnologies Corporation Diagnose von lymphoid-malignität und nachweis minimaler resterkrankungen
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
US10077478B2 (en) 2012-03-05 2018-09-18 Adaptive Biotechnologies Corp. Determining paired immune receptor chains from frequency matched subunits
KR101621643B1 (ko) 2012-05-08 2016-05-16 어댑티브 바이오테크놀로지스 코포레이션 다중 pcr 반응에서 증폭 바이어스를 측정 및 교정하기 위한 조성물 및 방법
EP3640343B1 (de) 2012-10-01 2026-01-07 Adaptive Biotechnologies Corporation Immunkompetenzbeurteilung durch adaptive immunrezeptordiversität und klonalitätscharakterisierung
WO2015160439A2 (en) 2014-04-17 2015-10-22 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
US20160230156A1 (en) 2013-03-15 2016-08-11 Life Technologies Corporation Recombinant fusion protein possessing nuclease and phosphatase activity
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
ES2741740T3 (es) 2014-03-05 2020-02-12 Adaptive Biotechnologies Corp Métodos que usan moléculas sintéticas que contienen segmentos de nucleótidos aleatorios
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
GB201414745D0 (en) 2014-08-19 2014-10-01 Articzymes As Exonucleases
WO2016053881A1 (en) 2014-10-03 2016-04-07 Life Technologies Corporation Genetic sequence verification compositions, methods and kits
EP3715455A1 (de) 2014-10-29 2020-09-30 Adaptive Biotechnologies Corp. Hochmultiplexierter gleichzeitiger nachweis von für adaptive gepaarte immunrezeptorheterodimere codierenden nukleinsäuren aus einer vielzahl von proben
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
WO2016086029A1 (en) 2014-11-25 2016-06-02 Adaptive Biotechnologies Corporation Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
US10302494B2 (en) 2014-12-18 2019-05-28 Palo Alto Research Center Incorporated Obtaining spectral information from a moving object
US10048192B2 (en) * 2014-12-18 2018-08-14 Palo Alto Research Center Incorporated Obtaining spectral information from moving objects
ES3002665T3 (en) 2015-02-24 2025-03-07 Adaptive Biotechnologies Corp Methods for diagnosing infectious disease and determining hla status using immune repertoire sequencing
AU2016242967B2 (en) 2015-04-01 2021-07-01 Adaptive Biotechnologies Corp. Method of identifying human compatible T cell receptors specific for an antigenic target
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4851331A (en) * 1986-05-16 1989-07-25 Allied Corporation Method and kit for polynucleotide assay including primer-dependant DNA polymerase
US4795699A (en) * 1987-01-14 1989-01-03 President And Fellows Of Harvard College T7 DNA polymerase
US5075216A (en) * 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
DE3923895A1 (de) * 1989-07-19 1991-01-24 Basf Ag Verfahren zur sequenzierung von desoxyribonukleinsaeuren
ES2141088T3 (es) * 1990-02-16 2000-03-16 Hoffmann La Roche Mejoras en la especificidad y conveniencia de la reaccion en cadena de la polimerasa.

Also Published As

Publication number Publication date
CA2119140A1 (en) 1993-04-01
WO1993006243A1 (en) 1993-04-01
EP0654091B1 (de) 2001-01-24
JP2695045B2 (ja) 1997-12-24
EP0654091A1 (de) 1995-05-24
DE69233041T2 (de) 2004-03-11
ES2156861T3 (es) 2001-08-01
DE69233041D1 (de) 2003-06-05
US5674679A (en) 1997-10-07
EP1038973B1 (de) 2003-05-02
EP1038973A1 (de) 2000-09-27
DE69231664T2 (de) 2001-05-03
US5741676A (en) 1998-04-21
EP0654091A4 (de) 1997-11-12
US5741640A (en) 1998-04-21
DE69231664D1 (de) 2001-03-01
ATE198913T1 (de) 2001-02-15
JPH06510433A (ja) 1994-11-24
AU2573992A (en) 1993-04-27

Similar Documents

Publication Publication Date Title
DE69233041D1 (de) Behandlung von amplifizierter DNS mit alkalischer Phosphatase
DE59007374D1 (de) Optisch gegengekoppelter Verstärker.
DE3873112D1 (de) Abwasserbehandlungsverfahren.
DE3874974D1 (de) Cmos-leistungsoperationsverstaerker.
DE3750667D1 (de) Leistungsverstärker.
DE3853136D1 (de) Operationsverstärker mit stabilem Arbeitspunkt.
DE3889831D1 (de) Laser-Apparat.
DE3879600D1 (de) Operationsverstaerker.
DE3880227D1 (de) Vorbeugung der nachoxidaton von methacrolein.
FI870147A0 (fi) Mikrobiologisk framstaellningsmetod.
DE3854236D1 (de) Laser-Apparat.
MA21139A1 (fr) Methode herbicide utilisant le diflufenican .
DE3768847D1 (de) Offset-korrigierter verstaerker.
FI892394L (fi) Enantioselektivt foerfarande.
DE3850923D1 (de) Operationsverstärkerstufen.
FI880320L (fi) Foerfarande foer framstaellning av n,n-(dibensohexatrienylen) urinaemnen.
SE8803182L (sv) Snabbloesbar koppling
DE58909170D1 (de) Transkonduktanzverstärker.
DK192890D0 (da) 24r-scumnol, fremgangsmaade til fremstilling heraf samt anvendelse af samme
DE3862356D1 (de) Bremskraftverstaerker.
DE3887916D1 (de) Schaltung zum automatischen Regeln des Verstärkung-Bandbreite-Produktes von Operationsverstärkern.
AU7879487A (en) Proteine protease inhibitors from streptomyces
DE3851515D1 (de) Metalldampf-Laser-Apparat.
DE69023704D1 (de) Verstärkerschaltung.
DE69114949D1 (de) Gegentakt-Leistungsverstärker.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties