DD254212A5 - Verfahren zur herstellung eines hefehybrivectors, der einen oder mehrere DNS-inserts enthalt - Google Patents

Verfahren zur herstellung eines hefehybrivectors, der einen oder mehrere DNS-inserts enthalt Download PDF

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Publication number
DD254212A5
DD254212A5 DD29389486A DD29389486A DD254212A5 DD 254212 A5 DD254212 A5 DD 254212A5 DD 29389486 A DD29389486 A DD 29389486A DD 29389486 A DD29389486 A DD 29389486A DD 254212 A5 DD254212 A5 DD 254212A5
Authority
DD
German Democratic Republic
Prior art keywords
dna
yeast
hybrid
promoter
gene
Prior art date
Application number
DD29389486A
Other languages
German (de)
English (en)
Inventor
Albert Hinnen
Bernd Meyhack
Original Assignee
Ciba Geigy Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ciba Geigy Ag filed Critical Ciba Geigy Ag
Publication of DD254212A5 publication Critical patent/DD254212A5/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424Serine endopeptidases (3.4.21)
    • C12N9/6456Plasminogen activators
    • C12N9/6459Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/65Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81Protease inhibitors
    • C07K14/815Protease inhibitors from leeches, e.g. hirudin, eglin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)
    • C12Y304/21069Protein C activated (3.4.21.69)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Mycology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Alcoholic Beverages (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Gyroscopes (AREA)
  • Led Devices (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Graft Or Block Polymers (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
DD29389486A 1985-08-29 1986-08-27 Verfahren zur herstellung eines hefehybrivectors, der einen oder mehrere DNS-inserts enthalt DD254212A5 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB858521496A GB8521496D0 (en) 1985-08-29 1985-08-29 Repressible yeast promoters

Publications (1)

Publication Number Publication Date
DD254212A5 true DD254212A5 (de) 1988-02-17

Family

ID=10584428

Family Applications (2)

Application Number Title Priority Date Filing Date
DD29389486A DD254212A5 (de) 1985-08-29 1986-08-27 Verfahren zur herstellung eines hefehybrivectors, der einen oder mehrere DNS-inserts enthalt
DD31325586A DD267739A5 (de) 1985-08-29 1986-08-27 Verfahren zur Herstellung eines zu Hefe heterologen Poly-Peptids

Family Applications After (1)

Application Number Title Priority Date Filing Date
DD31325586A DD267739A5 (de) 1985-08-29 1986-08-27 Verfahren zur Herstellung eines zu Hefe heterologen Poly-Peptids

Country Status (22)

Country Link
US (1) US5436136A (enExample)
EP (1) EP0213593B1 (enExample)
JP (1) JPH07110233B2 (enExample)
AT (1) ATE62510T1 (enExample)
AU (1) AU599329B2 (enExample)
CA (1) CA1318616C (enExample)
DD (2) DD254212A5 (enExample)
DE (1) DE3678647D1 (enExample)
DK (1) DK175093B1 (enExample)
ES (3) ES2001618A6 (enExample)
FI (1) FI91280C (enExample)
GB (1) GB8521496D0 (enExample)
GR (1) GR862209B (enExample)
HU (1) HU211207B (enExample)
IE (1) IE58844B1 (enExample)
IL (1) IL79837A (enExample)
NO (1) NO175871C (enExample)
NZ (1) NZ217388A (enExample)
PH (1) PH24715A (enExample)
PT (1) PT83273B (enExample)
SU (1) SU1630616A3 (enExample)
ZA (1) ZA866535B (enExample)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63501767A (ja) * 1985-11-13 1988-07-21 ゾマ、コーポレーション 短縮化されたホスホグリセリン酸キナ−ゼプロモ−タ−
GB8530631D0 (en) 1985-12-12 1986-01-22 Ciba Geigy Ag Thrombin inhibitors
MY101203A (en) * 1985-12-12 1991-08-17 Ucp Gen Pharma Ag Production of thrombin iinhibitors.
GB8620926D0 (en) * 1986-08-29 1986-10-08 Delta Biotechnology Ltd Yeast promoter
GB8907110D0 (en) * 1988-05-04 1989-05-10 Ciba Geigy Ag Improvements in the production of polypeptides
NO177065C (no) * 1988-09-26 1995-07-12 Labofina Sa Framgangsmåte for framstilling av enzymatisk aktivt humant lysozym
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
JPH06500470A (ja) * 1990-09-04 1994-01-20 メルク エンド カンパニー インコーポレーテッド メチロトローフ酵母細胞におけるインスリン様成長因子の産生
ES2177528T3 (es) * 1991-04-01 2002-12-16 Merck & Co Inc Genes que influyen en la actividad proteolitica de pichia y usoso de los mismos.
US5674728A (en) * 1993-11-03 1997-10-07 Novartis Corporation Aspergillus niger vacuolar aspartyl protease
JPH08173170A (ja) * 1994-05-25 1996-07-09 Kirin Brewery Co Ltd キャンディダ・ユティリス酵母の形質転換系、およびそれによる異種遺伝子の発現
ATE284972T1 (de) * 1996-09-24 2005-01-15 Cadus Pharmaceutical Corp Verfahren und zusammensetzungen für die identifizierung von receptor effectoren
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
US6890730B1 (en) 1999-12-10 2005-05-10 The Salk Institute Sequence and method for increasing protein expression in cellular expression systems
US8008459B2 (en) * 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
EP2571991A1 (en) 2010-05-20 2013-03-27 Evolva SA Method of producing isoprenoid compounds in yeast
BR112014022195A2 (pt) 2012-03-09 2020-05-12 Vestaron Corporation Produção de peptídeo tóxico, expressão de peptídeo em plantas e combinações de peptídeos ricos em cisteína
KR20230013034A (ko) 2020-04-20 2023-01-26 베스타론 코포레이션 해충 제어를 위한 단백분해 안정형 u1-아가톡신-ta1b 변이체 폴리펩티드
TW202205956A (zh) 2020-05-01 2022-02-16 美商韋斯塔隆公司 殺蟲組合
US20250188136A1 (en) 2021-04-01 2025-06-12 Vestaron Corporation Av3 mutant polypeptides for pest control
WO2023122805A1 (en) 2021-12-20 2023-06-29 Vestaron Corporation Sorbitol driven selection pressure method
EP4526330A1 (en) 2022-05-18 2025-03-26 Vestaron Corporation Pesticidal actx peptide variants
WO2023245100A1 (en) 2022-06-17 2023-12-21 Vestaron Corporation Antimicrobial ncr13 variant peptides
WO2024026406A2 (en) 2022-07-29 2024-02-01 Vestaron Corporation Next Generation ACTX Peptides
WO2024259306A2 (en) 2023-06-16 2024-12-19 Vestaron Corporation Pvd1 variant polypeptides for pest control
WO2025226701A1 (en) 2024-04-23 2025-10-30 Vestaron Corporation Proteolytically stable u1-agatoxin-ta1b variant polypeptides for pest control

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2125047B (en) * 1982-08-09 1986-02-19 Ciba Geigy Ag Yeast hybrid vectors and their use for the production of polypeptides
AU577259B2 (en) * 1982-08-13 1988-09-22 Zymogenetics Inc. Glycolytic promters for regulated protein expression protease inhibitor
US4876197A (en) * 1983-02-22 1989-10-24 Chiron Corporation Eukaryotic regulatable transcription
NZ207926A (en) * 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
ATE78293T1 (de) * 1983-05-27 1992-08-15 Texas A & M Univ Sys Verfahren zur herstellung eines rekombinanten baculovirus-expressionsvektors.
GB8314961D0 (en) * 1983-05-31 1983-07-06 Kingsman A J Dna sequence
EP0143081B1 (en) * 1983-11-21 1989-08-23 Ciba-Geigy Ag Synthesis of tissue plasminogen activator(tpa) by yeast
EP0164566A3 (de) * 1984-05-11 1986-10-29 LGA Gastechnik GmbH Sicherheitseinrichtung für Flüssiggastanks
ATE102250T1 (de) * 1984-05-11 1994-03-15 Chiron Corp Erhoehte hefetranskription unter verwendung einer hybridkonstruktion der promotorregion.
US4895800A (en) * 1985-11-26 1990-01-23 Phillips Petroleum Company Yeast production of hepatitis B surface antigen

Also Published As

Publication number Publication date
ZA866535B (en) 1987-04-29
EP0213593A1 (en) 1987-03-11
JPH07110233B2 (ja) 1995-11-29
PT83273B (pt) 1989-05-12
NZ217388A (en) 1989-02-24
NO175871C (no) 1994-12-21
IL79837A (en) 1992-06-21
GB8521496D0 (en) 1985-10-02
ES2006382A6 (es) 1989-04-16
FI91280C (fi) 1994-06-10
PH24715A (en) 1990-10-01
DK409686D0 (da) 1986-08-28
IE862307L (en) 1987-02-28
US5436136A (en) 1995-07-25
FI91280B (fi) 1994-02-28
AU6203286A (en) 1987-03-05
JPS6251995A (ja) 1987-03-06
EP0213593B1 (en) 1991-04-10
IE58844B1 (en) 1993-11-17
HU211207B (en) 1995-11-28
GR862209B (en) 1986-12-31
NO863458L (no) 1987-03-02
DD267739A5 (de) 1989-05-10
SU1630616A3 (ru) 1991-02-23
IL79837A0 (en) 1986-11-30
FI863430A0 (fi) 1986-08-25
FI863430L (fi) 1987-03-01
DE3678647D1 (en) 1991-05-16
ES2006383A6 (es) 1989-04-16
DK409686A (da) 1987-03-01
NO863458D0 (no) 1986-08-28
NO175871B (enExample) 1994-09-12
DK175093B1 (da) 2004-05-24
ES2001618A6 (es) 1988-06-01
PT83273A (en) 1986-09-01
HUT42525A (en) 1987-07-28
CA1318616C (en) 1993-06-01
ATE62510T1 (de) 1991-04-15
AU599329B2 (en) 1990-07-19

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