DE69428719T2 - Für das yap3 signalpeptid kodierendes dna-konstrukt - Google Patents

Für das yap3 signalpeptid kodierendes dna-konstrukt

Info

Publication number
DE69428719T2
DE69428719T2 DE69428719T DE69428719T DE69428719T2 DE 69428719 T2 DE69428719 T2 DE 69428719T2 DE 69428719 T DE69428719 T DE 69428719T DE 69428719 T DE69428719 T DE 69428719T DE 69428719 T2 DE69428719 T2 DE 69428719T2
Authority
DE
Germany
Prior art keywords
dna
pct
signal peptide
sequence encoding
dna sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69428719T
Other languages
English (en)
Other versions
DE69428719D1 (de
Inventor
Lars Christiansen
Jens G Litske Petersen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Application granted granted Critical
Publication of DE69428719D1 publication Critical patent/DE69428719D1/de
Publication of DE69428719T2 publication Critical patent/DE69428719T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8103—Exopeptidase (E.C. 3.4.11-19) inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69428719T 1993-07-08 1994-07-08 Für das yap3 signalpeptid kodierendes dna-konstrukt Expired - Lifetime DE69428719T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK93828A DK82893D0 (da) 1993-07-08 1993-07-08 Peptid
PCT/DK1994/000281 WO1995002059A1 (en) 1993-07-08 1994-07-08 A dna construct encoding the yap3 signal peptide

Publications (2)

Publication Number Publication Date
DE69428719D1 DE69428719D1 (de) 2001-11-22
DE69428719T2 true DE69428719T2 (de) 2002-08-08

Family

ID=8098010

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69428719T Expired - Lifetime DE69428719T2 (de) 1993-07-08 1994-07-08 Für das yap3 signalpeptid kodierendes dna-konstrukt

Country Status (20)

Country Link
US (1) US5726038A (de)
EP (1) EP0792367B1 (de)
JP (1) JP3542604B2 (de)
KR (1) KR100245173B1 (de)
AT (1) ATE207124T1 (de)
AU (1) AU7122194A (de)
CA (1) CA2166684A1 (de)
DE (1) DE69428719T2 (de)
DK (2) DK82893D0 (de)
ES (1) ES2165881T3 (de)
HU (1) HU222406B1 (de)
IL (1) IL110238A (de)
NZ (1) NZ268346A (de)
PL (1) PL178924B1 (de)
PT (1) PT792367E (de)
RU (1) RU2143495C1 (de)
SG (1) SG52452A1 (de)
UA (1) UA40625C2 (de)
WO (1) WO1995002059A1 (de)
ZA (1) ZA944912B (de)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2686899B1 (fr) * 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
US5861267A (en) * 1995-05-01 1999-01-19 Vertex Pharmaceuticals Incorporated Methods, nucleotide sequences and host cells for assaying exogenous and endogenous protease activity
US5772629A (en) * 1995-10-23 1998-06-30 Localmed, Inc. Localized intravascular delivery of TFPI for inhibition of restenosis in recanalized blood vessels
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
JP4275741B2 (ja) 1996-12-13 2009-06-10 ノバルティス バクシンズ アンド ダイアグノスティックス， インコーポレーテッド 血小板由来増殖因子タンパク質の分析および分離
JP2001527387A (ja) * 1997-01-24 2001-12-25 ノボ ノルディスク アクティーゼルスカブ 合成リーダーペプチド配列
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
AU4239600A (en) * 1999-04-30 2000-11-17 Ortho-Mcneil Pharmaceutical Research Inc. Zymogen activation system
US6420157B1 (en) * 1999-04-30 2002-07-16 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
KR20030004315A (ko) 1999-12-29 2003-01-14 노보 노르디스크 에이/에스 효모에서 개선된 발효 수율을 갖는 인슐린 선구물질 및인슐린 선구물질 유사체의 제조 방법
US20050100991A1 (en) * 2001-04-12 2005-05-12 Human Genome Sciences, Inc. Albumin fusion proteins
JP2003531590A (ja) * 2000-04-12 2003-10-28 ヒューマン ゲノム サイエンシズ インコーポレイテッド アルブミン融合タンパク質
US20030143191A1 (en) * 2001-05-25 2003-07-31 Adam Bell Chemokine beta-1 fusion proteins
EP1997829A1 (de) * 2001-12-21 2008-12-03 Human Genome Sciences, Inc. Albumin Fusionsproteine
EP1463752A4 (de) * 2001-12-21 2005-07-13 Human Genome Sciences Inc Albuminfusionsproteine
US7262287B2 (en) * 2002-06-29 2007-08-28 Korea Research Institute Of Bioscience And Biotechnology Hansenula polymorpha yapsin deficient mutant strain and process for the preparation of recombinant proteins using the same
ATE492631T1 (de) 2002-09-13 2011-01-15 Cornell Res Foundation Inc Verwendung von mutationen zur verbesserung von aspergillus-phytasen
KR100470978B1 (ko) * 2003-01-02 2005-03-10 한국생명공학연구원 앱신 다중 결손 효모 변이 균주 및 이를 이용한 재조합 단백질의 생산 방법
JP2007532096A (ja) 2003-11-14 2007-11-15 ノボ　ノルディスク　アクティーゼルスカブ アシル化されたインスリンの製造方法
AU2004295023A1 (en) 2003-12-03 2005-06-16 Novo Nordisk A/S Single-chain insulin
SI1729795T1 (sl) * 2004-02-09 2016-04-29 Human Genome Sciences, Inc. Albuminski fuzijski proteini
EP1917363B1 (de) 2005-08-16 2011-06-22 Novo Nordisk A/S Verfahren zur herstellung reifer insulinpolypeptide
US8722620B2 (en) 2006-02-27 2014-05-13 Novo Nordisk A/S Insulin derivatives
JP5503968B2 (ja) 2006-09-27 2014-05-28 ノボ・ノルデイスク・エー／エス 成熟インスリンポリペプチドの作製方法
US8153395B2 (en) 2006-11-22 2012-04-10 Novo Nordisk A/S Method of making activated carboxypeptidases
EP2170945A1 (de) 2007-07-16 2010-04-07 Novo Nordisk A/S Proteasestabilisierte pegylierte insulinanaloga
EP2910570B1 (de) 2008-03-18 2016-10-12 Novo Nordisk A/S Proteasestabilisierte, acylierte Insulinanaloga
EP2258855A1 (de) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expressionssequenzen
EP2649178B8 (de) 2010-12-09 2017-08-30 Institut Pasteur Mgmt-basierte methode zur erzielung hoher ausbeute bei rekombinanter proteinexpression
DE102011007313A1 (de) * 2011-04-13 2012-10-18 Henkel Ag & Co. Kgaa Expressionsverfahren
DE102011118032A1 (de) * 2011-05-31 2012-12-06 Henkel Ag & Co. Kgaa Expressionsvektoren zur verbesserten Proteinsekretion
CN104114697A (zh) 2011-12-09 2014-10-22 巴斯德研究所 多重免疫筛选分析
CN104837857B (zh) 2012-10-29 2018-11-06 龙沙有限公司 表达序列
WO2014088836A1 (en) 2012-12-03 2014-06-12 Merck Sharp & Dohme Corp. O-glycosylated carboxy terminal portion (ctp) peptide-based insulin and insulin analogues
CN105308067B (zh) 2013-06-07 2020-07-24 诺和诺德股份有限公司 制备成熟胰岛素多肽的方法
PL233560B1 (pl) 2014-12-05 2019-10-31 Mabion Spolka Akcyjna Sposób otrzymywania insuliny lub analogu insuliny z prekursora rekombinowanego białka
EP3268384B1 (de) 2015-03-10 2021-11-03 Merck Sharp & Dohme Corp. Verfahren zur herstellung von rekombinantem insulin unter verwendung von mikrofiltration
EA037848B1 (ru) 2016-07-14 2021-05-27 Общество С Ограниченной Ответственностью "Биохимический Агент" Гибридный белок, полинуклеотид, генетическая конструкция, продуцент, препарат для регенерации хряща (варианты)
WO2019099615A1 (en) 2017-11-17 2019-05-23 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Use of copeptin to modulate the immune response and hematopoiesis, and stimulate bone growth
WO2022049409A1 (en) 2020-09-02 2022-03-10 Sia Terragen Express diagnosticum for sars-cov-2
CN112920280B (zh) * 2021-04-22 2022-07-05 江南大学 一种酸性蛋白酶高效表达的方法及其应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0243465A1 (de) * 1985-10-25 1987-11-04 Vivian L. Mackay Verfahren zur verwendung von bar-1 zum abscheiden von fremdproteinen
US5217891A (en) * 1987-07-28 1993-06-08 Chiron Corporation DNA constructs containing a kluyveromyces α factor leader sequence for directing secretion of heterologous polypeptides
US5037743A (en) * 1988-08-05 1991-08-06 Zymogenetics, Inc. BAR1 secretion signal
US5538863A (en) * 1993-07-01 1996-07-23 Immunex Corporation Expression system comprising mutant yeast strain and expression vector encoding synthetic signal peptide

Also Published As

Publication number Publication date
RU2143495C1 (ru) 1999-12-27
ATE207124T1 (de) 2001-11-15
ES2165881T3 (es) 2002-04-01
PL312436A1 (en) 1996-04-29
JP3542604B2 (ja) 2004-07-14
KR100245173B1 (en) 2000-02-15
DK82893D0 (da) 1993-07-08
HUT74253A (en) 1996-11-28
NZ268346A (en) 1997-05-26
HU222406B1 (hu) 2003-06-28
US5726038A (en) 1998-03-10
JPH08512202A (ja) 1996-12-24
PL178924B1 (pl) 2000-06-30
IL110238A (en) 2003-04-10
CA2166684A1 (en) 1995-01-19
IL110238A0 (en) 1994-10-21
HU9600053D0 (en) 1996-03-28
EP0792367B1 (de) 2001-10-17
PT792367E (pt) 2002-04-29
SG52452A1 (en) 1998-09-28
UA40625C2 (uk) 2001-08-15
DE69428719D1 (de) 2001-11-22
AU7122194A (en) 1995-02-06
ZA944912B (en) 1995-03-27
DK0792367T3 (da) 2001-12-31
WO1995002059A1 (en) 1995-01-19
EP0792367A1 (de) 1997-09-03

Similar Documents

Publication Publication Date Title
DE69428719D1 (de) Für das yap3 signalpeptid kodierendes dna-konstrukt
DK72593D0 (da) Rekombinant protein
GB2301365B (en) Albumin secreted from yeast having reduced levels of aspartyl protease 3 proteolytic activity, and Yap3p-resistant albumin
DK0423302T3 (da) Anvendelse af hidtil ukendte DNA-fragmenter som kodende sekvens for et signalpeptid til sekretion af modne proteiner i rekombinant gær, ekspressionskassetter, transformeret gær og tilsvarende fremgangsmåde til fremstilling af proteiner
DE69532283D1 (de) EIN PILZ, IN DEM DAS areA-GEN VERÄNDERT IST, UND EIN areA-GEN AUS ASPERGILLUS ORYZAE
HU9502920D0 (en) New bacillus thuringiensis strains and their insecticidal proteins
BR8506902A (pt) Segmento de toxina de gene de proteina cristal bacillus thuringiensis
ATE108205T1 (de) Rekombinantverfahren zur herstellung von serinproteaseinhibitoren, sowie dns-sequenzen dazu.
PT755447E (pt) Proteinas recombinantes de hemaglutinina filamentosa de bordetella particularmente bordetella pertussis metodo para a sua producao e suas aplicaoes na producao de proteinas estranhas ou de principios activos para vacinas
DE69125381D1 (de) Proteinstruktur des pflanzlichen toxins gelonin
FR2692907B1 (fr) Levures kluyveromyces modifiees, preparation et utilisation.
DE60132571D1 (de) Für den menschlichen vanilloid-rezeptor vr3 kodierende dns
DE69133340D1 (de) Neue type-i-interferon varianten, ihre verfahren zur herstellung, und ihre verwendungen
NO962023D0 (no) Endotelin-konverterende enzym
ATE291629T1 (de) Expressionssystem für die sekretion von bioaktivem, menschlichem granulozyt-makrophagem koloniestimulierungsfaktor (gm-csf) und anderen heterologen proteinen aus streptomyces

Legal Events

Date Code Title Description
8364 No opposition during term of opposition