ES2165881T3 - Constructo de adn que codifica el peptido señal yap3. - Google Patents

Constructo de adn que codifica el peptido señal yap3.

Info

Publication number
ES2165881T3
ES2165881T3 ES94920419T ES94920419T ES2165881T3 ES 2165881 T3 ES2165881 T3 ES 2165881T3 ES 94920419 T ES94920419 T ES 94920419T ES 94920419 T ES94920419 T ES 94920419T ES 2165881 T3 ES2165881 T3 ES 2165881T3
Authority
ES
Spain
Prior art keywords
pct
signal peptide
sequence encoding
dna sequence
yap3
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94920419T
Other languages
English (en)
Inventor
Lars Christiansen
Jens G Litske Petersen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Application granted granted Critical
Publication of ES2165881T3 publication Critical patent/ES2165881T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8103—Exopeptidase (E.C. 3.4.11-19) inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

UNA CONSTRUCCION DE ADN QUE COMPRENDE LA SIGUIENTE SECUENCIA: 5''-P-SP-(()LP()){SUB,N}-PS-HP-3'' EN DONDE P ES UNA SECUENCIA DE PROMOTOR, SP ES UNA SECUENCIA DE ADN QUE CODIFICA EL PEPTIDO DE SEÑAL DE PROTEASA 3 DE LEVADURA ASPARTICA (YAP3), LP ES UNA SECUENCIA DE ADN QUE CODIFICA UN PEPTIDO DIRECTOR, N ES 0 O 1, PS ES UNA SECUENCIA DE ADN QUE CODIFICA UN PEPTIDO QUE DEFINE UN EMPLAZAMIENTO DE PROCESAMIENTO DE LEVADURA, Y HP ES UNA SECUENCIA DE ADN QUE CODIFICA UN POLIPEPTIDO QUE ES HETEROLOGO A UN ORGANISMO HUESPED SELECCIONADO. EL PEPTIDO DE SEÑAL YAP3 PROPORCIONA SECRECION EFICAZ DE PROTEINAS HETEROLOGAS EN LEVADURA.
ES94920419T 1993-07-08 1994-07-08 Constructo de adn que codifica el peptido señal yap3. Expired - Lifetime ES2165881T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DK93828A DK82893D0 (da) 1993-07-08 1993-07-08 Peptid

Publications (1)

Publication Number Publication Date
ES2165881T3 true ES2165881T3 (es) 2002-04-01

Family

ID=8098010

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94920419T Expired - Lifetime ES2165881T3 (es) 1993-07-08 1994-07-08 Constructo de adn que codifica el peptido señal yap3.

Country Status (20)

Country Link
US (1) US5726038A (es)
EP (1) EP0792367B1 (es)
JP (1) JP3542604B2 (es)
KR (1) KR100245173B1 (es)
AT (1) ATE207124T1 (es)
AU (1) AU7122194A (es)
CA (1) CA2166684A1 (es)
DE (1) DE69428719T2 (es)
DK (2) DK82893D0 (es)
ES (1) ES2165881T3 (es)
HU (1) HU222406B1 (es)
IL (1) IL110238A (es)
NZ (1) NZ268346A (es)
PL (1) PL178924B1 (es)
PT (1) PT792367E (es)
RU (1) RU2143495C1 (es)
SG (1) SG52452A1 (es)
UA (1) UA40625C2 (es)
WO (1) WO1995002059A1 (es)
ZA (1) ZA944912B (es)

Families Citing this family (42)

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FR2686899B1 (fr) * 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
US5861267A (en) * 1995-05-01 1999-01-19 Vertex Pharmaceuticals Incorporated Methods, nucleotide sequences and host cells for assaying exogenous and endogenous protease activity
US5772629A (en) * 1995-10-23 1998-06-30 Localmed, Inc. Localized intravascular delivery of TFPI for inhibition of restenosis in recanalized blood vessels
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
JP4341859B2 (ja) 1996-12-13 2009-10-14 ノバルティス バクシンズ アンド ダイアグノスティックス， インコーポレーテッド 酵母での異種タンパク質の発現の方法
AU5549998A (en) * 1997-01-24 1998-08-18 Novo Nordisk A/S Synthetic leader peptide sequences
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
JP2003531567A (ja) * 1999-04-30 2003-10-28 オーソ−マクニール・フアーマシユーチカル・インコーポレーテツド チモーゲン活性化系
US6420157B1 (en) * 1999-04-30 2002-07-16 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
RU2002120513A (ru) 1999-12-29 2004-03-27 Ново Нордиск А/С (DK) Способ производства предшественников инсулина и аналогов предшественников инсулина с повышенным выходом ферментации у дрожжей
US20050100991A1 (en) * 2001-04-12 2005-05-12 Human Genome Sciences, Inc. Albumin fusion proteins
US6926898B2 (en) * 2000-04-12 2005-08-09 Human Genome Sciences, Inc. Albumin fusion proteins
CA2446739A1 (en) * 2001-05-25 2002-12-05 Human Genome Sciences, Inc. Chemokine beta-1 fusion proteins
ES2500918T3 (es) * 2001-12-21 2014-10-01 Human Genome Sciences, Inc. Proteínas de fusión de albúmina e interferón beta
EP1463752A4 (en) * 2001-12-21 2005-07-13 Human Genome Sciences Inc ALBUMIN FUSION PROTEINS
ATE480624T1 (de) * 2002-06-29 2010-09-15 Korea Res Inst Of Bioscience Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit
EP1604008B1 (en) 2002-09-13 2010-12-22 Cornell Research Foundation, Inc. Using mutations to improve aspergillus phytases
KR100470978B1 (ko) * 2003-01-02 2005-03-10 한국생명공학연구원 앱신 다중 결손 효모 변이 균주 및 이를 이용한 재조합 단백질의 생산 방법
WO2005047508A1 (en) 2003-11-14 2005-05-26 Novo Nordisk A/S Processes for making acylated insulin
JP5697831B2 (ja) 2003-12-03 2015-04-08 ノヴォ ノルディスク アー／エス 単鎖インシュリン
SI1729795T1 (sl) * 2004-02-09 2016-04-29 Human Genome Sciences, Inc. Albuminski fuzijski proteini
EP1917363B1 (en) 2005-08-16 2011-06-22 Novo Nordisk A/S Method for making mature insulin polypeptides
DE602007009496D1 (de) 2006-02-27 2010-11-11 Novo Nordisk As Insulinderivate
EP2069502B1 (en) 2006-09-27 2014-02-26 Novo Nordisk A/S Method for making maturated insulin polypeptides
ES2425413T3 (es) 2006-11-22 2013-10-15 Novo Nordisk A/S Método para preparar carboxipeptidasa activada
CN101743252A (zh) 2007-07-16 2010-06-16 诺沃-诺迪斯克有限公司 蛋白酶稳定化的、peg化的胰岛素类似物
JP5749155B2 (ja) 2008-03-18 2015-07-15 ノボ・ノルデイスク・エー／エス プロテアーゼ安定化アシル化インスリンアナログ
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
CA2819552C (en) 2010-12-09 2023-09-26 Institut Pasteur Mgmt-based method for obtaining high yield of recombinant protein expression
DE102011007313A1 (de) * 2011-04-13 2012-10-18 Henkel Ag & Co. Kgaa Expressionsverfahren
DE102011118032A1 (de) * 2011-05-31 2012-12-06 Henkel Ag & Co. Kgaa Expressionsvektoren zur verbesserten Proteinsekretion
NO2788478T3 (es) 2011-12-09 2018-01-20
HUE033282T2 (en) 2012-10-29 2017-11-28 Lonza Ag Expression sequences
CA2890048C (en) 2012-12-03 2022-05-03 Merck Sharp & Dohme Corp. O-glycosylated carboxy terminal portion (ctp) peptide-based insulin and insulin analogues
EP3004156A1 (en) 2013-06-07 2016-04-13 Novo Nordisk A/S Method for making mature insulin polypeptides
PL233560B1 (pl) 2014-12-05 2019-10-31 Mabion Spolka Akcyjna Sposób otrzymywania insuliny lub analogu insuliny z prekursora rekombinowanego białka
WO2016144658A1 (en) 2015-03-10 2016-09-15 Merck Sharp & Dohme Corp. Process for preparing recombinant insulin using microfiltration
EA037848B1 (ru) 2016-07-14 2021-05-27 Общество С Ограниченной Ответственностью "Биохимический Агент" Гибридный белок, полинуклеотид, генетическая конструкция, продуцент, препарат для регенерации хряща (варианты)
WO2019099615A1 (en) 2017-11-17 2019-05-23 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Use of copeptin to modulate the immune response and hematopoiesis, and stimulate bone growth
WO2022049409A1 (en) 2020-09-02 2022-03-10 Sia Terragen Express diagnosticum for sars-cov-2
CN112920280B (zh) * 2021-04-22 2022-07-05 江南大学 一种酸性蛋白酶高效表达的方法及其应用

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WO1987002670A1 (en) * 1985-10-25 1987-05-07 Mackay Vivian L Method of using bar1 for secreting foreign proteins
US5217891A (en) * 1987-07-28 1993-06-08 Chiron Corporation DNA constructs containing a kluyveromyces α factor leader sequence for directing secretion of heterologous polypeptides
US5037743A (en) * 1988-08-05 1991-08-06 Zymogenetics, Inc. BAR1 secretion signal
US5538863A (en) * 1993-07-01 1996-07-23 Immunex Corporation Expression system comprising mutant yeast strain and expression vector encoding synthetic signal peptide

Also Published As

Publication number Publication date
ZA944912B (en) 1995-03-27
ATE207124T1 (de) 2001-11-15
PL178924B1 (pl) 2000-06-30
DK0792367T3 (da) 2001-12-31
US5726038A (en) 1998-03-10
UA40625C2 (uk) 2001-08-15
RU2143495C1 (ru) 1999-12-27
EP0792367B1 (en) 2001-10-17
CA2166684A1 (en) 1995-01-19
HU222406B1 (hu) 2003-06-28
HU9600053D0 (en) 1996-03-28
DE69428719T2 (de) 2002-08-08
PL312436A1 (en) 1996-04-29
WO1995002059A1 (en) 1995-01-19
IL110238A (en) 2003-04-10
KR100245173B1 (en) 2000-02-15
JP3542604B2 (ja) 2004-07-14
DK82893D0 (da) 1993-07-08
EP0792367A1 (en) 1997-09-03
JPH08512202A (ja) 1996-12-24
AU7122194A (en) 1995-02-06
IL110238A0 (en) 1994-10-21
DE69428719D1 (de) 2001-11-22
NZ268346A (en) 1997-05-26
PT792367E (pt) 2002-04-29
SG52452A1 (en) 1998-09-28
HUT74253A (en) 1996-11-28

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