ES2006382A6 - Procedimiento para la preparacion de un polipeptido mediante levadura que contiene un promotor hibrido con los puntos de activacion aguas arriba del gen pho5 de levadura. - Google Patents
Procedimiento para la preparacion de un polipeptido mediante levadura que contiene un promotor hibrido con los puntos de activacion aguas arriba del gen pho5 de levadura.Info
- Publication number
- ES2006382A6 ES2006382A6 ES8800794A ES8800794A ES2006382A6 ES 2006382 A6 ES2006382 A6 ES 2006382A6 ES 8800794 A ES8800794 A ES 8800794A ES 8800794 A ES8800794 A ES 8800794A ES 2006382 A6 ES2006382 A6 ES 2006382A6
- Authority
- ES
- Spain
- Prior art keywords
- yeast
- yeast promoters
- hybrid promoters
- repressible
- repressible yeast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/65—Insulin-like growth factors (Somatomedins), e.g. IGF-1, IGF-2
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
Abstract
PROCEDIMIENTO PARA LA PREPARACION DE UN POLIPEPTIDO MEDIANTE LEVADURA QUE CONTIENE UN PROMOTOR HIBRIDO CON LOS PUNTOS DE ACTIVACION AGUAS ARRIBA DEL GEN PH05 DE LEVADURA, CARACTERIZADO PORQUE SE CULTIVA LA CEPA DE LEVADURA TRANSFORMADA CON VECTOR HIBRIDO QUE CONTIENE UNO O MULTIPLES INSERTOS DE DNA, COMPRENDIENDO CADA UNO DE ELLOS UN SEGMENTO DE DNA QUE CODIFICA UN POLIPEPTIDO HETEROLOGO A LEVADURA, BAJO EL CONTROL TRANSCRIPCIONAL DE UN PROMOTOR HIBRIDO CONSISTENTE EN UN ELEMENTO PROMOTOR AGUAS ARRIBA 5'' CON UAS(S) DEL GEN PH05 DE LEVADURA Y UN ELEMENTO PROMOTOR AGUAS ABAJO 3'' DE UN GEN DE LEVADURA DISTINTO DEL GEN PH05 QUE COMPRENDE PUNTOS DE INICIACION DE LA TRANSCRIPCION INCLUYENDO UNA CAJA TATA FUNCIONAL, O UN MUTANTE DEL MISMO; Y SE AISLA EL POLIPEPTIDO ASI EXPRESADO.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB858521496A GB8521496D0 (en) | 1985-08-29 | 1985-08-29 | Repressible yeast promoters |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2006382A6 true ES2006382A6 (es) | 1989-04-16 |
Family
ID=10584428
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES8601490A Expired ES2001618A6 (es) | 1985-08-29 | 1986-08-29 | Procedimiento para la preparacion de promotores de levadura represibles conteniendo puntos de actividad agua arriba del gen phos de levadura. |
ES8800794A Expired ES2006382A6 (es) | 1985-08-29 | 1988-03-16 | Procedimiento para la preparacion de un polipeptido mediante levadura que contiene un promotor hibrido con los puntos de activacion aguas arriba del gen pho5 de levadura. |
ES8800795A Expired ES2006383A6 (es) | 1985-08-29 | 1988-03-16 | Procedimiento para la preparacion de vectores hibridos de levadura comprendiendo promotores de levadura represibles con los lugares de actuacion aguas arriba del gen ph05 de levadura. |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES8601490A Expired ES2001618A6 (es) | 1985-08-29 | 1986-08-29 | Procedimiento para la preparacion de promotores de levadura represibles conteniendo puntos de actividad agua arriba del gen phos de levadura. |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES8800795A Expired ES2006383A6 (es) | 1985-08-29 | 1988-03-16 | Procedimiento para la preparacion de vectores hibridos de levadura comprendiendo promotores de levadura represibles con los lugares de actuacion aguas arriba del gen ph05 de levadura. |
Country Status (22)
Country | Link |
---|---|
US (1) | US5436136A (es) |
EP (1) | EP0213593B1 (es) |
JP (1) | JPH07110233B2 (es) |
AT (1) | ATE62510T1 (es) |
AU (1) | AU599329B2 (es) |
CA (1) | CA1318616C (es) |
DD (2) | DD267739A5 (es) |
DE (1) | DE3678647D1 (es) |
DK (1) | DK175093B1 (es) |
ES (3) | ES2001618A6 (es) |
FI (1) | FI91280C (es) |
GB (1) | GB8521496D0 (es) |
GR (1) | GR862209B (es) |
HU (1) | HU211207B (es) |
IE (1) | IE58844B1 (es) |
IL (1) | IL79837A (es) |
NO (1) | NO175871C (es) |
NZ (1) | NZ217388A (es) |
PH (1) | PH24715A (es) |
PT (1) | PT83273B (es) |
SU (1) | SU1630616A3 (es) |
ZA (1) | ZA866535B (es) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0245479B1 (en) * | 1985-11-13 | 1993-02-17 | Xoma Corporation | Shortened phosphoglycerate kinase promoter |
MY101203A (en) * | 1985-12-12 | 1991-08-17 | Ucp Gen Pharma Ag | Production of thrombin iinhibitors. |
GB8530631D0 (en) | 1985-12-12 | 1986-01-22 | Ciba Geigy Ag | Thrombin inhibitors |
GB8620926D0 (en) * | 1986-08-29 | 1986-10-08 | Delta Biotechnology Ltd | Yeast promoter |
GB8907110D0 (en) * | 1988-05-04 | 1989-05-10 | Ciba Geigy Ag | Improvements in the production of polypeptides |
NO177065C (no) * | 1988-09-26 | 1995-07-12 | Labofina Sa | Framgangsmåte for framstilling av enzymatisk aktivt humant lysozym |
WO1992004363A1 (en) * | 1990-09-04 | 1992-03-19 | The Salk Institute Biotechnology/Industrial Associates, Inc. | Production of insulin-like growth factor-1 in methylotrophic yeast cells |
US5612198A (en) * | 1990-09-04 | 1997-03-18 | The Salk Institute | Production of insulin-like growth factor-1 in methylotrophic yeast cells |
EP0578746B1 (en) * | 1991-04-01 | 2002-07-03 | Merck & Co., Inc. | GENES WHICH INFLUENCE $i(PICHIA) PROTEOLYTIC ACTIVITY, AND USES THEREFOR |
US5674728A (en) * | 1993-11-03 | 1997-10-07 | Novartis Corporation | Aspergillus niger vacuolar aspartyl protease |
JPH08173170A (ja) * | 1994-05-25 | 1996-07-09 | Kirin Brewery Co Ltd | キャンディダ・ユティリス酵母の形質転換系、およびそれによる異種遺伝子の発現 |
EP0929691B1 (en) * | 1996-09-24 | 2004-12-15 | Cadus Pharmaceutical Corporation | Methods and compositions for identifying receptor effectors |
US6265186B1 (en) | 1997-04-11 | 2001-07-24 | Dsm N.V. | Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces |
US6890730B1 (en) | 1999-12-10 | 2005-05-10 | The Salk Institute | Sequence and method for increasing protein expression in cellular expression systems |
US8008459B2 (en) * | 2001-01-25 | 2011-08-30 | Evolva Sa | Concatemers of differentially expressed multiple genes |
WO2011146833A1 (en) | 2010-05-20 | 2011-11-24 | Evolva Inc. | Method of producing isoprenoid compounds in yeast |
PE20231212A1 (es) | 2020-04-20 | 2023-08-17 | Vestaron Corp | Polipeptidos variantes proteoliticamente estables de u1-agatoxina-ta1b para el control de plagas |
EP4142498A1 (en) | 2020-05-01 | 2023-03-08 | Vestaron Corporation | Insecticidal combinations |
BR112023019825A2 (pt) | 2021-04-01 | 2024-01-16 | Vestaron Corp | Polipeptídeos mutantes av3 para controle de pragas |
WO2023122805A1 (en) | 2021-12-20 | 2023-06-29 | Vestaron Corporation | Sorbitol driven selection pressure method |
WO2023225555A1 (en) | 2022-05-18 | 2023-11-23 | Vestaron Corporation | Pesticidal actx peptide variants |
WO2023245100A1 (en) | 2022-06-17 | 2023-12-21 | Vestaron Corporation | Antimicrobial ncr13 variant peptides |
WO2024026406A2 (en) | 2022-07-29 | 2024-02-01 | Vestaron Corporation | Next Generation ACTX Peptides |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2125047B (en) * | 1982-08-09 | 1986-02-19 | Ciba Geigy Ag | Yeast hybrid vectors and their use for the production of polypeptides |
AU577259B2 (en) * | 1982-08-13 | 1988-09-22 | Zymogenetics Inc. | Glycolytic promters for regulated protein expression protease inhibitor |
US4876197A (en) * | 1983-02-22 | 1989-10-24 | Chiron Corporation | Eukaryotic regulatable transcription |
NZ207926A (en) * | 1983-04-25 | 1988-04-29 | Genentech Inc | Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast |
EP0127839B1 (en) * | 1983-05-27 | 1992-07-15 | THE TEXAS A&M UNIVERSITY SYSTEM | Method for producing a recombinant baculovirus expression vector |
GB8314961D0 (en) * | 1983-05-31 | 1983-07-06 | Kingsman A J | Dna sequence |
PH22337A (en) * | 1983-11-21 | 1988-08-12 | Ciba Geigy Ag | Synthesis of fibrinolytic agents by yeast |
EP0164566A3 (de) * | 1984-05-11 | 1986-10-29 | LGA Gastechnik GmbH | Sicherheitseinrichtung für Flüssiggastanks |
EP0480480A3 (en) * | 1984-05-11 | 1992-06-10 | Chiron Corporation | Enhanced yeast transcription employing hybrid promoter region constructs |
US4895800A (en) * | 1985-11-26 | 1990-01-23 | Phillips Petroleum Company | Yeast production of hepatitis B surface antigen |
-
1985
- 1985-08-29 GB GB858521496A patent/GB8521496D0/en active Pending
-
1986
- 1986-08-25 IL IL79837A patent/IL79837A/xx not_active IP Right Cessation
- 1986-08-25 FI FI863430A patent/FI91280C/fi not_active IP Right Cessation
- 1986-08-26 AT AT86111820T patent/ATE62510T1/de not_active IP Right Cessation
- 1986-08-26 EP EP86111820A patent/EP0213593B1/en not_active Expired - Lifetime
- 1986-08-26 DE DE8686111820T patent/DE3678647D1/de not_active Expired - Lifetime
- 1986-08-27 CA CA000516879A patent/CA1318616C/en not_active Expired - Lifetime
- 1986-08-27 DD DD31325586A patent/DD267739A5/de unknown
- 1986-08-27 DD DD29389486A patent/DD254212A5/de not_active IP Right Cessation
- 1986-08-27 GR GR862209A patent/GR862209B/el unknown
- 1986-08-28 DK DK198604096A patent/DK175093B1/da not_active IP Right Cessation
- 1986-08-28 SU SU864028035A patent/SU1630616A3/ru active
- 1986-08-28 AU AU62032/86A patent/AU599329B2/en not_active Expired
- 1986-08-28 PT PT83273A patent/PT83273B/pt unknown
- 1986-08-28 ZA ZA866535A patent/ZA866535B/xx unknown
- 1986-08-28 NZ NZ217388A patent/NZ217388A/xx unknown
- 1986-08-28 PH PH34192A patent/PH24715A/en unknown
- 1986-08-28 HU HU863720A patent/HU211207B/hu unknown
- 1986-08-28 IE IE230786A patent/IE58844B1/en not_active IP Right Cessation
- 1986-08-28 NO NO863458A patent/NO175871C/no not_active IP Right Cessation
- 1986-08-29 JP JP61201924A patent/JPH07110233B2/ja not_active Expired - Lifetime
- 1986-08-29 ES ES8601490A patent/ES2001618A6/es not_active Expired
-
1988
- 1988-03-16 ES ES8800794A patent/ES2006382A6/es not_active Expired
- 1988-03-16 ES ES8800795A patent/ES2006383A6/es not_active Expired
-
1991
- 1991-12-20 US US07/811,898 patent/US5436136A/en not_active Expired - Lifetime
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PC1A | Transfer granted | ||
FD1A | Patent lapsed |
Effective date: 20060830 |