JPS642579A - Recombinant dna, microorganism containing said dna and production of polypeptide therewith - Google Patents
Recombinant dna, microorganism containing said dna and production of polypeptide therewithInfo
- Publication number
- JPS642579A JPS642579A JP15674587A JP15674587A JPS642579A JP S642579 A JPS642579 A JP S642579A JP 15674587 A JP15674587 A JP 15674587A JP 15674587 A JP15674587 A JP 15674587A JP S642579 A JPS642579 A JP S642579A
- Authority
- JP
- Japan
- Prior art keywords
- dna
- gastrin
- recombinant dna
- coding
- yeast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/595—Gastrins; Cholecystokinins [CCK]
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Toxicology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Endocrinology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
PURPOSE:To produce gastrin in a large amount, by transforming an yeast with a recombinant DNA containing a region capable of coding the gastrin, promoter region and region capable of coding a signal peptide and cultivating the transformed yeast. CONSTITUTION:A recombinant DNA is obtained by integrating a DNA fragment capable of coding gastrin which is a hormone having promoting action on gas tric acid secretion into a suitable shuttle vector. An yeast is then transformed therewith and cultivated to secrete the gastrin.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62-156745A JPH012579A (en) | 1987-06-24 | Recombinant DNA, microorganisms containing it, and methods for producing polypeptides using the same |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62-156745A JPH012579A (en) | 1987-06-24 | Recombinant DNA, microorganisms containing it, and methods for producing polypeptides using the same |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS642579A true JPS642579A (en) | 1989-01-06 |
JPH012579A JPH012579A (en) | 1989-01-06 |
Family
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