IL90947A - Method for production of hiv protease in transformed cells, dna and vectors comprising said dna used in said production - Google Patents

Method for production of hiv protease in transformed cells, dna and vectors comprising said dna used in said production

Info

Publication number
IL90947A
IL90947A IL90947A IL9094789A IL90947A IL 90947 A IL90947 A IL 90947A IL 90947 A IL90947 A IL 90947A IL 9094789 A IL9094789 A IL 9094789A IL 90947 A IL90947 A IL 90947A
Authority
IL
Israel
Prior art keywords
dna
production
vectors
sequence
transformed cells
Prior art date
Application number
IL90947A
Other versions
IL90947A0 (en
Original Assignee
Us Commerce
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Commerce filed Critical Us Commerce
Publication of IL90947A0 publication Critical patent/IL90947A0/en
Publication of IL90947A publication Critical patent/IL90947A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A method of producing a recombinant protein having proteolytic activity, comprising the steps of: (i)introducing into a host cell the DNA gene sequence consisting essentially of: 102030405060 CCTCAGATCA CTCTTTGGCA ACGACCCCTC GTCACAATAA AGATAGGGGG GCAACTAAAG Pro 708090100110120 GAAGCTCTAT TAGATACAGG AGCAGATGAT ACAGTATTAG AAGAAATGAG TTTGCCAGGA 130140150160170180 AGATGGAAAC CAAAAATGAT AGGGGGAATT GGAGGTTTTA TCAAAGTAAG ACAGTATGAT 190200210220230240 CAGATACTCA TAGAAATCTG TGGACATAAA GCTATAGGTA CAGTATTAGT AGGACCTACA 250260270280290 CCTGTCAACA TAATTGGAAG AAATCTGTTG ACTCAGATTG CTTGCACTTT AAATTT Phe or allelic or species variations thereof, said variations encoding the same amino acid sequence as the DNA sequence, said DNA sequence operatively linked to a promoter; (ii)culturing said host cell under conditions such that said sequence or allelic or species variations thereof is expressed and said protein thereby produced, and (iii)isolating said protein.
IL90947A 1988-07-13 1989-07-12 Method for production of hiv protease in transformed cells, dna and vectors comprising said dna used in said production IL90947A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US21830488A 1988-07-13 1988-07-13

Publications (2)

Publication Number Publication Date
IL90947A0 IL90947A0 (en) 1990-02-09
IL90947A true IL90947A (en) 1997-06-10

Family

ID=22814563

Family Applications (1)

Application Number Title Priority Date Filing Date
IL90947A IL90947A (en) 1988-07-13 1989-07-12 Method for production of hiv protease in transformed cells, dna and vectors comprising said dna used in said production

Country Status (6)

Country Link
EP (1) EP0425580A4 (en)
JP (1) JPH04500602A (en)
AU (1) AU635216B2 (en)
CA (1) CA1340748C (en)
IL (1) IL90947A (en)
WO (1) WO1990000556A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69231726D1 (en) * 1991-12-24 2001-04-12 Amrad Corp Ltd USE OF LEUKEMIA INHIBITING FACTOR (LIF) FOR THE TREATMENT OF TUMORS AND SARCOMAS
RU2126794C1 (en) * 1992-03-11 1999-02-27 Нархекс Лимитед Amino-derivatives of oxo- or hydroxy-substituted hydrazines, method of their synthesis and pharmaceutical compositions for inhibition of retrovirus protease
US5888992A (en) * 1992-03-11 1999-03-30 Narhex Limited Polar substituted hydrocarbons
ATE253050T1 (en) 1992-03-11 2003-11-15 Narhex Ltd AMINE DERIVATIVES OF OXO- AND HYDROXY-SUBSTITUTED HYDROCARBONS
US6071895A (en) * 1992-03-11 2000-06-06 Narhex Limited Polar-substituted hydrocarbons
US6001558A (en) * 1997-06-25 1999-12-14 Ortho Clinical Diagnostics, Inc. Amplification and detection of HIV-1 and/or HIV 2
ATE384795T1 (en) 1998-12-31 2008-02-15 Novartis Vaccines & Diagnostic MODIFIED HIV ENV POLYPEPTIDES
CA2360347C (en) 1998-12-31 2013-05-07 Chiron Corporation Improved expression of hiv polypeptides and production of virus-like particles
US7935805B1 (en) 1998-12-31 2011-05-03 Novartis Vaccines & Diagnostics, Inc Polynucleotides encoding antigenic HIV Type C polypeptides, polypeptides and uses thereof
EP1141314A2 (en) 1998-12-31 2001-10-10 Chiron Corporation Polynucleotides encoding antigenic hiv type c polypeptides, polypeptides and uses thereof
AU2002316578A1 (en) 2001-08-31 2003-03-18 Chiron Corporation Polynucleotides encoding antigenic hiv type b polypeptides, polypeptides and uses thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5252477A (en) * 1987-06-01 1993-10-12 The United States Of America As Represented By The United States Department Of Health And Human Services Human immunodeficiency virus specific proteolytic enzyme and a method for its synthesis and renaturation

Also Published As

Publication number Publication date
CA1340748C (en) 1999-09-14
EP0425580A4 (en) 1992-05-06
WO1990000556A1 (en) 1990-01-25
AU4058589A (en) 1990-02-05
JPH04500602A (en) 1992-02-06
EP0425580A1 (en) 1991-05-08
IL90947A0 (en) 1990-02-09
AU635216B2 (en) 1993-03-18

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