SE8204811L - DNA FRAGMENT INCLUDING A SIGNAL SEQUENCE - Google Patents

DNA FRAGMENT INCLUDING A SIGNAL SEQUENCE

Info

Publication number
SE8204811L
SE8204811L SE8204811A SE8204811A SE8204811L SE 8204811 L SE8204811 L SE 8204811L SE 8204811 A SE8204811 A SE 8204811A SE 8204811 A SE8204811 A SE 8204811A SE 8204811 L SE8204811 L SE 8204811L
Authority
SE
Sweden
Prior art keywords
gene
preparation
coding
polypeptide
protein
Prior art date
Application number
SE8204811A
Other languages
Unknown language ( )
Swedish (sv)
Other versions
SE8204811D0 (en
Inventor
Sven Lofdahl
Mathias Uhlen
Martin Lindberg
Original Assignee
Sven Lofdahl
Mathias Uhlen
Martin Lindberg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sven Lofdahl, Mathias Uhlen, Martin Lindberg filed Critical Sven Lofdahl
Priority to SE8204811A priority Critical patent/SE8204811L/en
Publication of SE8204811D0 publication Critical patent/SE8204811D0/en
Priority to DE19833390106 priority patent/DE3390106T1/en
Priority to JP50289083A priority patent/JPS59501694A/en
Priority to PCT/SE1983/000298 priority patent/WO1984000774A1/en
Publication of SE8204811L publication Critical patent/SE8204811L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A DNA-fragment which comprises a novel signal DNA sequence coding for a signal peptide and which downstream thereof comprises or can be provided with an insertion site for a gene or a portion thereof coding for a selected protein or polypeptide, such that expression of the signal DNA sequence together with said gene or portion thereof produces a precursor of the selected protein or polypeptide, from which the signal peptide is cleaved off during secretion of the protein or polypeptide product through a cell membrane. The signal DNA sequence is characterized by being derived from a staphylococcal protein A coding gene, or being a chemically synthetized equivalent thereto. A process for its preparation as well as a cloning vehicle comprising such a DNA-fragment and the preparation thereof are provided. Also a microorganism transformed by such a cloning vehicle and a process for the preparation thereof are provided. Finally there are provided a recombinant DNA molecule comprising such a cloning vehicle into which a gene or a portion thereof coding for a selected protein or polypeptide has been inserted, the process for preparing such a recombinant DNA molecule, a microorganism transformed thereby and a process for its preparation.
SE8204811A 1982-08-23 1982-08-23 DNA FRAGMENT INCLUDING A SIGNAL SEQUENCE SE8204811L (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
SE8204811A SE8204811L (en) 1982-08-23 1982-08-23 DNA FRAGMENT INCLUDING A SIGNAL SEQUENCE
DE19833390106 DE3390106T1 (en) 1982-08-23 1983-08-23 A DNA fragment which contains a signal DNA sequence, a method for its production and a microorganism transformed therewith
JP50289083A JPS59501694A (en) 1982-08-23 1983-08-23 DNA fragment containing a signal DNA sequence, its production method, and microorganisms transformed thereby
PCT/SE1983/000298 WO1984000774A1 (en) 1982-08-23 1983-08-23 Staphylococcal protein a coding gene (dna) fragment comprising a signal dna sequence, a process for its preparation and a microorganism transformed therewith

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE8204811A SE8204811L (en) 1982-08-23 1982-08-23 DNA FRAGMENT INCLUDING A SIGNAL SEQUENCE

Publications (2)

Publication Number Publication Date
SE8204811D0 SE8204811D0 (en) 1982-08-23
SE8204811L true SE8204811L (en) 1984-02-24

Family

ID=20347593

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8204811A SE8204811L (en) 1982-08-23 1982-08-23 DNA FRAGMENT INCLUDING A SIGNAL SEQUENCE

Country Status (4)

Country Link
JP (1) JPS59501694A (en)
DE (1) DE3390106T1 (en)
SE (1) SE8204811L (en)
WO (1) WO1984000774A1 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4737544A (en) * 1982-08-12 1988-04-12 Biospecific Technologies, Inc. Biospecific polymers
US5151350A (en) * 1982-10-27 1992-09-29 Repligen Corporation Cloned genes encoding recombinant protein a
SE8300693L (en) * 1983-02-09 1984-08-10 Sven Lofdahl SET TO MAKE AND ISOLATE PROTEINS AND POLYPEPTIDES, AND A HYBRID VECTOR FOR THIS
US4963495A (en) * 1984-10-05 1990-10-16 Genentech, Inc. Secretion of heterologous proteins
US4680262A (en) * 1984-10-05 1987-07-14 Genentech, Inc. Periplasmic protein recovery
EP0177343B1 (en) * 1984-10-05 1992-07-22 Genentech, Inc. Dna, cell cultures and methods for the secretion of heterologous proteins and periplasmic protein recovery
JPS61135599A (en) * 1984-12-04 1986-06-23 Green Cross Corp:The Production of heteroprotein
DK528285D0 (en) * 1985-11-15 1985-11-15 Novo Industri As PEPTIDES AND PREPARATIONS CONTAINING THESE
US5583024A (en) * 1985-12-02 1996-12-10 The Regents Of The University Of California Recombinant expression of Coleoptera luciferase
JPH01500322A (en) * 1986-03-28 1989-02-09 クリエイティブ　バイオモレクレス，インコーポレーテッド Tissue plasminogen activator related protein
JPH03503596A (en) * 1987-06-24 1991-08-15 ノボ・ノルディスク　エー／エス A method for preparing a protein or polypeptide, a DNA sequence encoding the polypeptide, a microorganism having the DNA sequence and the polypeptide, and use of the polypeptide as a pharmaceutical preparation
US5084398A (en) * 1987-11-20 1992-01-28 Creative Biomolecules Selective removal of immune complexes
US5243040A (en) * 1987-11-20 1993-09-07 Creative Biomolecules DNA encoding a protein which enables selective removal of immune complexes
WO2008127457A2 (en) 2006-12-06 2008-10-23 Repligen Corporation Nucleic acids encoding recombinant protein a

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI64814C (en) * 1980-12-31 1984-01-10 Alko Ab Oy EFFECTIVE EFFECTIVENESS OF PRODUCTION AND PRODUCTION
FI64813C (en) * 1980-12-31 1984-01-10 Ilkka Antero Palva FOERFARANDE FOER PRODUCERING AV ETT UTVALT AEGGVITEAEMNE OCH VID FOERFARANDET ANVAENDA REKOMBINANTPLASMIDVEKTORER

Also Published As

Publication number Publication date
DE3390106T1 (en) 1984-11-15
WO1984000774A1 (en) 1984-03-01
SE8204811D0 (en) 1982-08-23
JPS59501694A (en) 1984-10-11

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