CN1223679C - 提高微生物发酵生产乙醇的方法 - Google Patents
提高微生物发酵生产乙醇的方法 Download PDFInfo
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- CN1223679C CN1223679C CNB01813372XA CN01813372A CN1223679C CN 1223679 C CN1223679 C CN 1223679C CN B01813372X A CNB01813372X A CN B01813372XA CN 01813372 A CN01813372 A CN 01813372A CN 1223679 C CN1223679 C CN 1223679C
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- reactor
- gas
- ethanol
- cell
- acetate
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/04—Bioreactors or fermenters specially adapted for specific uses for producing gas, e.g. biogas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/12—Bioreactors or fermenters specially adapted for specific uses for producing fuels or solvents
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M43/00—Combinations of bioreactors or fermenters with other apparatus
- C12M43/02—Bioreactors or fermenters combined with devices for liquid fuel extraction; Biorefineries
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Abstract
Description
成分 | 每升含量 |
2g/l FeCl2·4H2O | 10ml |
85%H3PO4 | 0.05ml |
MPFN痕量矿物a | 20ml |
(NH4)2HPO4 | 0.60g |
NH4Cl | 2.00g |
NaCl | 0.20g |
KCl | 0.15g |
MgCl2·6H2O | 0.50g |
CaCl2·2H2O | 0.20g |
半胱氨酸HCl·H2O | 0.25g |
维生素溶液b | 可变c |
杨氏梭菌 | 培养基 | 细胞浓度* | 气体转化 | 产物浓度 | ||
菌株 | 限制 | (g/L) | CO | H2 | 乙醇 | 乙酸 |
ERI2 | 乙酸增强 | 1.1 | 90 | 80 | 0 | 10 |
ERI2 | 泛酸钙限制 | 0.3 | 88 | 20 | 2.5 | 0 |
ERI2 | 乙酸增强 | 0.55 | 90 | 85 | 1.0 | 5.5 |
ERI2 | 泛酸钙限制 | 0.5 | 90 | 20 | 10 | 1.0 |
ERI2 | 乙酸增强 | 0.8 | 100 | 93 | 1 | 7 |
ERI2 | 钴限制 | 1.3 | 80 | 20 | 9 | 3 |
C-01 | 乙酸增强 | 1.2 | 96 | 90 | 1 | 8 |
C-01 | 泛酸钙限制 | 0.8 | 60 | 30 | 4 | 0 |
C-01 | 钴限制 | 2.5 | 80 | 20 | 17 | 2 |
C-01 | 乙酸增强 | 1.2 | 96 | 90 | <1 | 9 |
PETC | 乙酸增强 | 0.8 | 65 | 55 | 2 | 10 |
PETC | 钴限制 | 1.0 | 95 | 55 | 8 | 1 |
GRT | LRT | 搅拌速率 | 细胞浓度* | 气体转化(%) | 产物(g/L) | 乙醇产率 | |||
(min) | (hr) | (rpm) | (g/L) | CO | H2 | 乙醇 | 乙酸 | (g/L·天) | |
13 | 32.4 | 700 | 2.44 | 91 | 57 | 21.6 | 3.9 | 16.0 | |
11.93 | 25.7 | 750 | 2.51 | 92 | 54 | 20.6 | 3.6 | 19.2 | |
12.67 | 25.6 | 750 | 2.60 | 93 | 61 | 18.7 | 4.7 | 17.6 | |
10 | 24.5 | 750 | 2.75 | 92 | 43 | 20.4 | 6.1 | 20.0 | |
11.54 | 23.8 | 750 | 2.65 | 92 | 40 | 20.4 | 5.3 | 20.6 | |
12.10 | 23.8 | 750 | 2.77 | 88 | 18 | 21 | 3.1 | 21.1 | |
13.8 | 23.8 | 750 | 2.70 | 90 | 25 | 18 | 2.5 | 18.2 | |
12.7 | 23.8 | 750 | 2.70 | 92 | 35 | 20 | 3.8 | 20.2 | |
13.3 | 24.0 | 800 | 2.70 | 85 | 10 | 17.5 | 5.0 | 17.5 | |
14.81 | 31 | 750 | 2.50 | 92 | 30 | 25 | 2.5 | 19.4 | |
16.9 | 31 | 750 | 3.60 | 90 | 18 | 23 | 3.0 | 17.8 | |
18.5 | 33 | 750 | 2.60 | 94 | 50 | 24 | 3.5 | 17.5 | |
17.2 | 34 | 750 | 2.50 | 91 | 40 | 24 | 3.5 | 16.9 | |
18.5 | 34 | 750 | 2.30 | 95 | 63 | 23 | 4.0 | 16.2 | |
19.2 | 40.6 | 750 | 2.70 | 94 | 50 | 28.5 | 4.0 | 17.4 | |
19.0 | 55 | 750 | 2.70 | 94 | 20 | 33 | 4.0 | 14.4 |
GRT | LRT | 搅拌速率 | 细胞浓度* | 气体转化(%) | 产物(g/L) | 乙醇产率 | ||
(min) | (hr) | (rpm) | (g/L) | CO | H2 | 乙醇 | 乙酸 | (g/L·天) |
8.89 | 23.8 | 750 | 2.3 | 84 | 57 | 11 | 2.5 | 11.1 |
8.3 | 23.8 | 900 | 2.6 | 89 | 55 | 12 | 2.0 | 12.1 |
8.3 | 27.7 | 900 | 2.7 | 89 | 47 | 15 | 3.0 | 13.0 |
7.1 | 33.3 | 900 | 3.0 | 86 | 37 | 19 | 3.0 | 13.7 |
7.4 | 33.3 | 900 | 3.0 | 87 | 40 | 19.5 | 3.0 | 14.1 |
6.34 | 33.3 | 900 | 3.0 | 86 | 37 | 21 | 3.5 | 15.1 |
6.18 | 33.3 | 900 | 3.0 | 86 | 41 | 20.9 | 3.1 | 15.1 |
5.72 | 34.3 | 900 | 3.0 | 85 | 40 | 22.1 | 3.8 | 15.5 |
5.12 | 33 | 900 | 3.7 | 85 | 40 | 25.0 | 4.0 | 18.2 |
4.59 | 33 | 900 | 4.1 | 83 | 33 | 25 | 3.5 | 18.2 |
4.59 | 29 | 900 | 4.0 | 80 | 35 | 23 | 4.0 | 19.0 |
4.76 | 29 | 900 | 3.9 | 90 | 35 | 19 | 5.0 | 15.7 |
4.25 | 28 | 900 | 4.2 | 80 | 30 | 23 | 3.0 | 19.7 |
5.5 | 37 | 900 | 3.4 | 84 | 40 | 23 | 3.0 | 14.9 |
5.26 | 31 | 900 | 3.8 | 84 | 50 | 23 | 3.0 | 17.8 |
5.71 | 31 | 900 | 3.7 | 80 | 28 | 26 | 3.5 | 20.1 |
6.25 | 31 | 900 | 3.75 | 82 | 30 | 25.5 | 3.0 | 19.7 |
6.66 | 31 | 900 | 3.6 | 86 | 64 | 22 | 4.0 | 17.0 |
GRT | XRT | LRT | 细胞浓度* | 气体转化(%) | 产物(g/L) | 乙醇产率 | ||
(min) | (hr) | (hr) | (g/L) | CO | H2 | 乙醇 | 乙酸 | (g/L·天) |
12.5 | 46.4 | 23.2 | 3.8 | 96.3 | 81.2 | 20.4 | 4.4 | 21.1 |
9.7 | 43.2 | 17.3 | 4.9 | 86.7 | 49.9 | 21.1 | 3.5 | 29.3 |
9.2 | 43.2 | 17.3 | 4.6 | 89.4 | 64.5 | 20.5 | 5.1 | 28.4 |
7.5 | 43.2 | 17.3 | 5.0 | 81.8 | 42.1 | 22.2 | 3.7 | 30.8 |
9.2 | 49.4 | 17.3 | 4.6 | 85.3 | 52.1 | 21.1 | 4.4 | 29.3 |
8.4 | 46.0 | 16.1 | 4.5 | 85.2 | 61.4 | 20.8 | 5.1 | 31.0 |
6.8 | 54.3 | 16.3 | 4.7 | 84.7 | 57.7 | 23.4 | 5.7 | 34.5 |
7.2 | 54.3 | 16.3 | 4.0 | 83.1 | 55.2 | 19.0 | 4.4 | 28.0 |
6.4 | 55.6 | 16.7 | 5.6 | 83.3 | 53.1 | 23.5 | 4.9 | 33.8 |
6.2 | 41.6 | 14.5 | 5.7 | 82.5 | 55.0 | 20.1 | 5.0 | 33.3 |
6.0 | 41.6 | 14.5 | 6.0 | 82.5 | 50.0 | 21.5 | 3.0 | 35.6 |
6.0 | 34.2 | 12.0 | 5.7 | 84.0 | 56.0 | 19.5 | 4.5 | 39.0 |
5.7 | 34.2 | 12.0 | 5.7 | 81.0 | 45.0 | 18.0 | 4.5 | 36.0 |
时间 | pH | 细胞浓度*(g/L) | 乙醇(g/L) | 乙酸(g/L) | 丁醇(g/L) |
0 | 4.69 | 2.4 | 10.3 | 3.1 | 0.3 |
30 | 5.28 | 11.4 | 1.5 | 0.3 | |
35 | 5.28 | 2.4 | 11.6 | 1.6 | 0.3 |
50 | 4.98 | 11.3 | 1.8 | 0.3 | |
80 | 4.73 | 2.4 | 10.9 | 2.9 | 0.3 |
时间(hr) | %水回收 | 细胞浓度*(g/L) | 气体转化 | 产物 | 乙醇产率 | |||
CO(%) | H2(%) | 乙醇(g/L) | 乙酸(g/L) | 纯乙酸(g/L) | (g/L·天) | |||
0-75 | 25 | 2.1 | 95 | 68 | 12 | 4 | 4 | 12 |
75-193 | 50 | 2.1 | 95 | 75 | 15 | 6 | 5 | 15 |
193-462 | 75 | 2.1 | 92 | 60 | 17 | 5 | 4 | 17 |
462-554 | 50 | 1.6 | 85 | 30 | 17→13 | 5 | 3 | 12-16 |
554-669 | 75 | 2.6 | 92 | 75 | 13→19 | 5 | 3 | 12-18 |
669-943 | 100 | 3.0 | 92 | 70 | 23 | 6 | 3 | 23 |
943-1087 | 100 | 3.0 | 92 | 60 | 23 | 6 | 0 | 23 |
1087-1232 | 100 | 2.7 | 92 | 60 | 23 | 6 | -0 | 23 |
1232-1375 | 100 | 3.0 | 91 | 60 | 27 | 6 | -1 | 27 |
1375-1534 | 100 | 3.5 | 88 | 35 | 23 | 5 | 0 | 23 |
Claims (22)
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CN105296543B (zh) * | 2011-06-30 | 2020-01-14 | 巨鹏生物股份公司 | 用于合成气发酵的生物反应器 |
CN104781408A (zh) * | 2012-05-22 | 2015-07-15 | 伊内奥斯生物股份公司 | 用于发酵含co的气态底物的方法 |
CN104781408B (zh) * | 2012-05-22 | 2018-04-24 | 伊内奥斯生物股份公司 | 用于发酵含co的气态底物的方法 |
CN105164267A (zh) * | 2013-02-14 | 2015-12-16 | 伊内奥斯生物股份公司 | 使含co气态底物发酵的方法 |
CN105164267B (zh) * | 2013-02-14 | 2019-01-18 | 伊内奥斯生物股份公司 | 使含co气态底物发酵的方法 |
TWI755353B (zh) * | 2014-10-22 | 2022-02-21 | 美商朗澤科技Nz有限公司 | 氣體測試單元及方法 |
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