CN1201016C - 用于监测dna聚合酶链反应的仪器 - Google Patents

用于监测dna聚合酶链反应的仪器 Download PDF

Info

Publication number
CN1201016C
CN1201016C CNB998061999A CN99806199A CN1201016C CN 1201016 C CN1201016 C CN 1201016C CN B998061999 A CNB998061999 A CN B998061999A CN 99806199 A CN99806199 A CN 99806199A CN 1201016 C CN1201016 C CN 1201016C
Authority
CN
China
Prior art keywords
emission
data
dye
excitation
detector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CNB998061999A
Other languages
English (en)
Chinese (zh)
Other versions
CN1309766A (zh
Inventor
M·R·干比尼
J·G·阿特伍德
E·F·杨
E·J·拉卡托斯
A·L·塞罗内
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
Pe Co Ny
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26773060&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CN1201016(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Pe Co Ny filed Critical Pe Co Ny
Publication of CN1309766A publication Critical patent/CN1309766A/zh
Application granted granted Critical
Publication of CN1201016C publication Critical patent/CN1201016C/zh
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1811—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using electromagnetic induction heating
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/101—Reactions characterised by their quantitative nature the purpose being quantitative analysis with an internal standard/control
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6482—Sample cells, cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/02—Mechanical
    • G01N2201/024—Modular construction
    • G01N2201/0245—Modular construction with insertable-removable part
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/061—Sources
    • G01N2201/06113—Coherent sources; lasers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/062—LED's
    • G01N2201/0628—Organic LED [OLED]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/063—Illuminating optical parts
    • G01N2201/0635—Structured illumination, e.g. with grating
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/063—Illuminating optical parts
    • G01N2201/0636—Reflectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/068—Optics, miscellaneous

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Theoretical Computer Science (AREA)
  • Clinical Laboratory Science (AREA)
  • Biotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Optics & Photonics (AREA)
  • Mathematical Physics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CNB998061999A 1998-05-16 1999-05-17 用于监测dna聚合酶链反应的仪器 Expired - Lifetime CN1201016C (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US8576598P 1998-05-16 1998-05-16
US60/085,765 1998-05-16
US9278498P 1998-07-14 1998-07-14
US60/092,784 1998-07-14

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN2005100548515A Division CN1664562A (zh) 1998-05-16 1999-05-17 用于监测dna聚合酶链反应的仪器

Publications (2)

Publication Number Publication Date
CN1309766A CN1309766A (zh) 2001-08-22
CN1201016C true CN1201016C (zh) 2005-05-11

Family

ID=26773060

Family Applications (2)

Application Number Title Priority Date Filing Date
CN2005100548515A Pending CN1664562A (zh) 1998-05-16 1999-05-17 用于监测dna聚合酶链反应的仪器
CNB998061999A Expired - Lifetime CN1201016C (zh) 1998-05-16 1999-05-17 用于监测dna聚合酶链反应的仪器

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CN2005100548515A Pending CN1664562A (zh) 1998-05-16 1999-05-17 用于监测dna聚合酶链反应的仪器

Country Status (9)

Country Link
US (1) US9671342B2 (OSRAM)
EP (4) EP1078245B1 (OSRAM)
JP (7) JP2003524754A (OSRAM)
CN (2) CN1664562A (OSRAM)
AT (2) ATE425448T1 (OSRAM)
AU (1) AU759974B2 (OSRAM)
CA (1) CA2328609A1 (OSRAM)
DE (2) DE69940574D1 (OSRAM)
WO (1) WO1999060381A1 (OSRAM)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107044886A (zh) * 2017-01-20 2017-08-15 武汉呵尔医疗科技发展有限公司 一种多波段光谱成像光源、成像方法及显微镜

Families Citing this family (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7560273B2 (en) * 2002-07-23 2009-07-14 Applied Biosystems, Llc Slip cover for heated platen assembly
US7498164B2 (en) 1998-05-16 2009-03-03 Applied Biosystems, Llc Instrument for monitoring nucleic acid sequence amplification reaction
US6818437B1 (en) 1998-05-16 2004-11-16 Applera Corporation Instrument for monitoring polymerase chain reaction of DNA
ATE425448T1 (de) 1998-05-16 2009-03-15 Applera Corp Optisches gerät insbesondere zur überwachung von dns-polymerasekettenreaktionen
US7387891B2 (en) 1999-05-17 2008-06-17 Applera Corporation Optical instrument including excitation source
US7423750B2 (en) 2001-11-29 2008-09-09 Applera Corporation Configurations, systems, and methods for optical scanning with at least one first relative angular motion and at least one second angular motion or at least one linear motion
US20050279949A1 (en) 1999-05-17 2005-12-22 Applera Corporation Temperature control for light-emitting diode stabilization
US7410793B2 (en) 1999-05-17 2008-08-12 Applera Corporation Optical instrument including excitation source
US6518068B1 (en) 1999-07-21 2003-02-11 Tropix, Inc. Luminescence detection workstation
EP1617207A3 (en) * 1999-07-21 2006-05-31 Applera Corporation Luminescence detection workstation
DK2258168T3 (da) 2000-05-09 2019-10-07 Xy Llc Apparat til isolering af X-kromosombærende og Y-kromosombærende populationer af spermatozoer
US6563581B1 (en) 2000-07-14 2003-05-13 Applera Corporation Scanning system and method for scanning a plurality of samples
JP3741051B2 (ja) * 2001-05-10 2006-02-01 横河電機株式会社 バイオチップ読取装置
US6594010B2 (en) * 2001-07-06 2003-07-15 Praxair Technology, Inc. Emission spectrometer having a charge coupled device detector
US6867005B2 (en) * 2001-10-24 2005-03-15 Beckman Coulter, Inc. Method and apparatus for increasing the dynamic range and accuracy of binding assays
US7635588B2 (en) 2001-11-29 2009-12-22 Applied Biosystems, Llc Apparatus and method for differentiating multiple fluorescence signals by excitation wavelength
DE10201463B4 (de) * 2002-01-16 2005-07-21 Clondiag Chip Technologies Gmbh Reaktionsgefäß zur Durchführung von Array-Verfahren
DE10204531A1 (de) * 2002-02-01 2003-08-21 Inst Chemo Biosensorik Deckelelement
US6982166B2 (en) 2002-05-16 2006-01-03 Applera Corporation Lens assembly for biological testing
US9157860B2 (en) 2002-05-16 2015-10-13 Applied Biosystems, Llc Achromatic lens array
ES2543587T3 (es) * 2002-05-17 2015-08-20 Life Technologies Corporation Instrumento óptico que incluye una fuente de excitación
EP2463644B1 (en) 2002-05-17 2013-10-09 Life Technologies Corporation Optical instrument including excitation source
KR100794699B1 (ko) * 2002-06-18 2008-01-14 (주)바이오니아 핵산증폭반응 산물의 실시간 모니터링 장치
US7083974B2 (en) 2002-07-12 2006-08-01 Applera Corporation Rotatable sample disk and method of loading a sample disk
US7452712B2 (en) 2002-07-30 2008-11-18 Applied Biosystems Inc. Sample block apparatus and method of maintaining a microcard on a sample block
US7148043B2 (en) 2003-05-08 2006-12-12 Bio-Rad Laboratories, Inc. Systems and methods for fluorescence detection with a movable detection module
EP2407243B1 (en) 2003-07-31 2020-04-22 Handylab, Inc. Multilayered microfluidic device
JP2007518109A (ja) * 2004-01-14 2007-07-05 アプレラ コーポレイション 生物学的サンプルにおける蛍光検出のための装置および方法
US7295316B2 (en) 2004-01-14 2007-11-13 Applera Corporation Fluorescent detector with automatic changing filters
ATE357653T1 (de) * 2005-01-18 2007-04-15 Hoffmann La Roche Fluoreszenzabbildung mittels telezentrischer anregungs- und abbildungsoptiken
EP1681558B1 (en) * 2005-01-18 2007-11-14 Roche Diagnostics GmbH Imaging fluorescence signals using telecentric optics on the excitation and the imaging side
EP1681556B1 (en) * 2005-01-18 2007-04-11 Roche Diagnostics GmbH Imaging fluorescence signals using telecentricity
KR20080108145A (ko) * 2005-01-24 2008-12-11 올림푸스 가부시키가이샤 생화학 검사 장치 및 생화학 검사 방법
FI20055043A0 (fi) * 2005-01-31 2005-01-31 Wallac Oy Sovitelma mikrotiterkaivojen kuvausjärjestelmässä
US7446288B2 (en) 2005-05-06 2008-11-04 Applied Biosystems Inc. Device including inductively heatable fluid retainment region, and method
DE102005027407B3 (de) * 2005-06-13 2006-11-09 Eppendorf Ag Thermocycler
DE102005027555B3 (de) * 2005-06-14 2006-10-05 Eppendorf Ag Thermocycler
DE102005043834A1 (de) * 2005-09-13 2007-03-22 Eppendorf Ag Vorrichtung zur Durchführung von Real-Time PCR-Reaktionen
JP2007093249A (ja) * 2005-09-27 2007-04-12 Yokogawa Electric Corp 光量計測装置および光量計測方法
JP4696959B2 (ja) * 2006-02-22 2011-06-08 アイシン精機株式会社 光学検出装置
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US9040288B2 (en) 2006-03-24 2015-05-26 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using the same
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
JP2008082922A (ja) * 2006-09-28 2008-04-10 Sanyo Electric Co Ltd 撮影装置及び細胞観察装置
EP2091647A2 (en) 2006-11-14 2009-08-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
JP4842175B2 (ja) 2007-03-07 2011-12-21 東京エレクトロン株式会社 温度測定装置及び温度測定方法
KR101089045B1 (ko) 2007-06-28 2011-12-02 (주)바이오니아 핵산증폭반응 산물의 실시간 모니터링장치
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
EP2108699B1 (en) 2008-04-08 2014-06-25 F.Hoffmann-La Roche Ag Analytical processing and detection device
US20110057120A1 (en) * 2008-06-05 2011-03-10 Bohle Ag Apparatus for determining the element occupancy on a surface by means of fluorescence
EP2148187A1 (de) 2008-07-25 2010-01-27 Roche Diagnostics GmbH Anregungs- und Abbildungsoptik für die Fluoreszenzdetektion
FR2938067B1 (fr) 2008-11-05 2010-12-31 Edixia Dispositif pour inspecter des soudures d'emballage.
WO2010110096A1 (ja) * 2009-03-26 2010-09-30 ユニバーサル・バイオ・リサーチ株式会社 反応光学測定装置およびその測定方法
CN102012357B (zh) * 2009-09-07 2012-12-12 复旦大学 可变光程的旁路浓度校准系统
EP2301666B1 (en) 2009-09-09 2021-06-30 Cole-Parmer Ltd. Optical system for multiple reactions
CN102791847B (zh) * 2010-01-12 2015-01-21 阿赫姆生物系统公司 三阶段热对流装置及其用途
JP2010281842A (ja) * 2010-09-24 2010-12-16 Yokogawa Electric Corp 光量計測装置および光量計測方法
JP2011017721A (ja) * 2010-09-24 2011-01-27 Yokogawa Electric Corp 光量計測装置および光量計測方法
JP2012085605A (ja) * 2010-10-22 2012-05-10 Sony Corp 核酸増幅反応装置、核酸増幅反応装置に用いる基板、及び核酸増幅反応方法
JP6088487B2 (ja) * 2011-04-15 2017-03-01 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ 走査リアルタイムマイクロ流体熱サイクラーと同期熱サイクリング及び走査光学検出の方法
JP5842586B2 (ja) * 2011-12-14 2016-01-13 凸版印刷株式会社 真贋検証器及び真贋検証方法
JP5690359B2 (ja) 2012-03-30 2015-03-25 株式会社Ｓｃｒｅｅｎホールディングス 撮像装置および撮像方法
JP6057060B2 (ja) * 2012-08-29 2017-01-11 株式会社ニデック 眼底撮影装置
JP6449591B2 (ja) 2013-09-02 2019-01-09 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 生物学的液体の光測定装置
CN104729994B (zh) * 2013-12-20 2018-05-29 福州高意通讯有限公司 用于增强拉曼光谱仪信噪比的方法和装置
CN105092544A (zh) * 2014-05-12 2015-11-25 绍兴安尼特微电子科技有限公司 一种荧光定量pcr检测仪光学激发和检测系统
CN107548462B (zh) * 2015-02-06 2020-12-18 生命技术公司 用于生物分析的系统和方法
CN113049553B (zh) 2015-02-06 2024-08-20 生命技术公司 用于评价生物样本的系统和方法
JP6622975B2 (ja) * 2015-03-13 2019-12-18 浜松ホトニクス株式会社 計測装置及び計測方法
KR101838640B1 (ko) * 2015-06-17 2018-03-14 (주)동국이노텍 마이크로플레이트 기반 세포 대사 측정 실험 장치
JP6747192B2 (ja) * 2016-09-07 2020-08-26 株式会社島津製作所 遺伝子増幅装置
EP3523038B1 (en) * 2016-10-07 2021-03-03 Boehringer Ingelheim Vetmedica GmbH Method for controlling an analysis device and analysis system
CN107746806B (zh) * 2017-11-20 2024-02-20 鲲鹏基因（北京）科技有限责任公司 一种实时荧光定量pcr仪
CN108181239B (zh) * 2018-02-07 2023-09-12 张哲夫 一种多通道荧光定量pcr仪的光学系统
IT201800004132A1 (it) * 2018-03-30 2019-09-30 St Microelectronics Srl Unita' di rilevamento di segnali fluorescenti, apparecchio di analisi di molecole che comprende tale unita' e metodo di rilevamento ed analisi dei segnali fluorescenti
KR102133633B1 (ko) * 2018-04-17 2020-07-13 (주)로고스바이오시스템스 핵산증폭반응산물을 실시간으로 검출하는 장치
WO2020014400A1 (en) * 2018-07-10 2020-01-16 Gen-Probe Incorporated Methods and systems for detecting and quantifying nucleic acids
TWI726446B (zh) * 2019-08-11 2021-05-01 新加坡商克雷多生物醫學私人有限公司 分析系統及其分析方法
CN113376127B (zh) * 2020-02-25 2025-03-28 上海迈景纳米科技有限公司 一种适于生物分子多重检测的成像系统
US20230296518A1 (en) * 2020-02-25 2023-09-21 Shanghai Jiao Tong University Imaging system for multiplex detection of biomolecules
US11480565B2 (en) 2020-06-12 2022-10-25 Bio-Rad Laboratories, Inc. Automated immunoassay
KR102409740B1 (ko) 2020-11-05 2022-06-20 (주)그린광학 텔레센트릭 광학계를 포함하는 형광 측정 장치
CN113481095A (zh) * 2021-07-12 2021-10-08 桂林电子科技大学 基于双芯光纤活体单细胞转动精准主动光操控方法及装置
EP4396338A1 (en) 2021-09-01 2024-07-10 Ludwig-Maximilians-Universität München Activatable dna polymerase
WO2025257247A1 (en) * 2024-06-12 2025-12-18 Roche Diagnostics International Ag Automated pcr analyzer

Family Cites Families (193)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2408646A1 (de) * 1974-02-22 1975-08-28 Max Planck Gesellschaft Reaktionskinetisches messgeraet
JPS53135660A (en) * 1977-04-30 1978-11-27 Olympus Optical Co Ltd Fluorescent photometric microscope using laser light
SU938936A1 (ru) * 1978-11-01 1982-06-30 Институт биологической физики АН СССР Устройство дл поиска измененных клеток в цитологическом препарате
US4200801A (en) * 1979-03-28 1980-04-29 The United States Of America As Represented By The United States Department Of Energy Portable spotter for fluorescent contaminants on surfaces
US4487477A (en) * 1981-03-06 1984-12-11 The Perkin-Elmer Corporation Optical beam splitter
US4412543A (en) 1981-04-09 1983-11-01 Xanar, Inc. Apparatus for determining the concentration of a fluorescent material in an eye
JPS57186169A (en) * 1981-05-12 1982-11-16 Olympus Optical Co Ltd Detector for particle coagulation pattern
US4537861A (en) * 1983-02-03 1985-08-27 Elings Virgil B Apparatus and method for homogeneous immunoassay
US4626684A (en) * 1983-07-13 1986-12-02 Landa Isaac J Rapid and automatic fluorescence immunoassay analyzer for multiple micro-samples
DE3329257A1 (de) * 1983-08-12 1985-02-28 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen Fluorometer
JPS60108457A (ja) 1983-11-16 1985-06-13 Japan Synthetic Rubber Co Ltd 透明耐衝撃性樹脂組成物
US4660971A (en) 1984-05-03 1987-04-28 Becton, Dickinson And Company Optical features of flow cytometry apparatus
FR2566543B1 (fr) * 1984-06-20 1988-02-26 Commissariat Energie Atomique Dispositif optique a rendement de collection eleve et cytofluorimetre en faisant application
US4661913A (en) 1984-09-11 1987-04-28 Becton, Dickinson And Company Apparatus and method for the detection and classification of articles using flow cytometry techniques
ATE48477T1 (de) 1984-09-11 1989-12-15 Partec Ag Verfahren und vorrichtung zur sortierung von mikroskopischen partikeln.
US5656493A (en) 1985-03-28 1997-08-12 The Perkin-Elmer Corporation System for automated performance of the polymerase chain reaction
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
JPS6216911U (OSRAM) * 1985-07-16 1987-01-31
US4989977A (en) 1985-07-29 1991-02-05 Becton, Dickinson And Company Flow cytometry apparatus with improved light beam adjustment
JPH0818666B2 (ja) 1985-11-26 1996-02-28 大同工業株式会社 コイル搬送装置
US4786165A (en) 1986-07-10 1988-11-22 Toa Medical Electronics Co., Ltd. Flow cytometry and apparatus therefor
JPS63287177A (ja) * 1987-05-19 1988-11-24 Canon Inc 撮像装置
DE3717274A1 (de) 1987-05-22 1988-12-01 Sick Erwin Gmbh Optische fehlerinspektionsvorrichtung
JP2550106B2 (ja) 1987-10-30 1996-11-06 株式会社日立製作所 光分散検出型電気泳動装置
US4887721A (en) 1987-11-30 1989-12-19 The United States Of America As Represented By The United States Department Of Energy Laser particle sorter
US4972258A (en) * 1989-07-31 1990-11-20 E. I. Du Pont De Nemours And Company Scanning laser microscope system and methods of use
GB8830039D0 (en) 1988-12-22 1989-02-15 Renishaw Plc Raman microscope
US5241363A (en) * 1989-07-10 1993-08-31 General Atomics Micropipette adaptor with temperature control for PCR amplification
US5030002A (en) 1989-08-11 1991-07-09 Becton, Dickinson And Company Method and apparatus for sorting particles with a moving catcher tube
US5018866A (en) * 1989-09-12 1991-05-28 Packard Instrument Company Method and apparatus for performing high sensitivity fluorescence measurements
JPH05500563A (ja) * 1989-09-27 1993-02-04 アストロスキャン・リミテッド 炭水化物の分析
US5091652A (en) * 1990-01-12 1992-02-25 The Regents Of The University Of California Laser excited confocal microscope fluorescence scanner and method
US5073029A (en) * 1990-02-16 1991-12-17 Eqm Research, Inc. Multisource device for photometric analysis and associated chromogens
EP0443700A3 (en) * 1990-02-21 1991-10-16 Mitsubishi Jukogyo Kabushiki Kaisha Method for counting living cells of microbes and apparatus therefor
DE4013586C2 (de) * 1990-04-27 1994-08-18 Suzuki Motor Co Vorrichtung zur Feststellung der immunologischen Agglutination
US5215883A (en) * 1990-07-09 1993-06-01 The Research Foundation Electrophoretic mobility of fluorophore labeled particles in gels by fluorophore movement after photobleaching
GB9015793D0 (en) * 1990-07-18 1990-09-05 Medical Res Council Confocal scanning optical microscope
US5127730A (en) * 1990-08-10 1992-07-07 Regents Of The University Of Minnesota Multi-color laser scanning confocal imaging system
US5141609A (en) 1990-11-16 1992-08-25 The Trustees Of The Leland Stanford Junior University Method and device employing time-delayed integration for detecting sample components after separation
KR100236506B1 (ko) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
DE4139211C2 (de) 1990-11-30 1994-03-24 Hitachi Ltd Elektrophoresegerät
US6352672B1 (en) * 1991-01-28 2002-03-05 Cis Bio International Apparatus for measuring the luminescence emitted in a luminescent assay
FR2672128B1 (fr) * 1991-01-28 1995-08-18 Cis Bio Int Procede de mesure de la luminescence emise dans un dosage par luminescence.
DE4102767A1 (de) * 1991-01-31 1992-08-06 Metallgesellschaft Ag Verfahren zur qualitativen analyse von kunststoffteilchen
US5817462A (en) * 1995-02-21 1998-10-06 Applied Spectral Imaging Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding
JPH04271800A (ja) 1991-02-28 1992-09-28 Hitachi Ltd 電気泳動装置
US5529679A (en) 1992-02-28 1996-06-25 Hitachi, Ltd. DNA detector and DNA detection method
US5516409A (en) 1991-02-28 1996-05-14 Hitachi, Ltd. DNA detector and DNA detection method
US5243540A (en) * 1991-04-03 1993-09-07 The United States Of America As Represented By The Secretary Of The Army Computer-driven amino acid indexer for peptide synthesis
EP0588972A4 (en) * 1991-06-13 1994-09-14 Abbott Lab Optical imaging for positioning and cell counting
JP2785530B2 (ja) 1991-09-13 1998-08-13 株式会社日立製作所 電気泳動装置
US5371624A (en) * 1991-11-29 1994-12-06 Olympus Optical Co., Ltd. Reflected fluorescence microscope
US5173748A (en) 1991-12-05 1992-12-22 Eastman Kodak Company Scanning multichannel spectrometry using a charge-coupled device (CCD) in time-delay integration (TDI) mode
US5670113A (en) * 1991-12-20 1997-09-23 Sibia Neurosciences, Inc. Automated analysis equipment and assay method for detecting cell surface protein and/or cytoplasmic receptor function using same
US5315375A (en) * 1992-02-11 1994-05-24 Acrogen, Inc. Sensitive light detection system
US5635402A (en) * 1992-03-05 1997-06-03 Alfano; Robert R. Technique for determining whether a cell is malignant as opposed to non-malignant using extrinsic fluorescence spectroscopy
AU4047893A (en) * 1992-04-06 1993-11-08 Abbott Laboratories Method and device for detection of nucleic acid or analyte using total internal reflectance
SE500702C2 (sv) 1992-04-07 1994-08-15 Staffan Birnbaum Sätt och anordning för optisk analys av prov separerade i tunna kapillärer
US5355215A (en) * 1992-09-30 1994-10-11 Environmental Research Institute Of Michigan Method and apparatus for quantitative fluorescence measurements
WO1994016104A1 (en) * 1993-01-08 1994-07-21 Ctrc Research Foundation Color imaging system for use in molecular biology
US5547849A (en) * 1993-02-17 1996-08-20 Biometric Imaging, Inc. Apparatus and method for volumetric capillary cytometry
US5383023A (en) * 1993-03-01 1995-01-17 Walleczek; Jan Method and apparatus for performing dual-beam dual-wavelength fluorescence spectrophotometric evaluation of a biological specimen
DE69323060T2 (de) 1993-03-18 1999-06-10 Novartis Ag, Basel Optische Detektorvorrichtung für die chemische Analyse von kleinen fluiden Probenvolumina
US5730850A (en) 1993-04-23 1998-03-24 Hitachi, Ltd. Capillary array electrophoresis system
US5371016A (en) * 1993-04-26 1994-12-06 Becton, Dickinson And Company Detecting biological activities in culture vials
US5512492A (en) * 1993-05-18 1996-04-30 University Of Utah Research Foundation Waveguide immunosensor with coating chemistry providing enhanced sensitivity
US5439578A (en) 1993-06-03 1995-08-08 The Governors Of The University Of Alberta Multiple capillary biochemical analyzer
CA2129787A1 (en) * 1993-08-27 1995-02-28 Russell G. Higuchi Monitoring multiple amplification reactions simultaneously and analyzing same
US5397709A (en) * 1993-08-27 1995-03-14 Becton Dickinson And Company System for detecting bacterial growth in a plurality of culture vials
JPH07120393A (ja) 1993-10-13 1995-05-12 Nippon Tectron Co Ltd 蛍光検出法
US5515169A (en) * 1993-10-13 1996-05-07 Labintelligence Inc. Spectral wavelength discrimination system and method for using
US5377004A (en) * 1993-10-15 1994-12-27 Kaiser Optical Systems Remote optical measurement probe
US5470710A (en) 1993-10-22 1995-11-28 University Of Utah Automated hybridization/imaging device for fluorescent multiplex DNA sequencing
JPH07120392A (ja) 1993-10-28 1995-05-12 Shimadzu Corp 塩基配列決定装置
US6309601B1 (en) * 1993-11-01 2001-10-30 Nanogen, Inc. Scanning optical detection system
JP3448090B2 (ja) * 1994-02-16 2003-09-16 浜松ホトニクス株式会社 エネルギー移動検出法およびその装置
WO1995023348A1 (en) 1994-02-25 1995-08-31 Massachusetts Institute Of Technology Methods and apparatus for detecting and imaging particles
US5491343A (en) * 1994-03-25 1996-02-13 Brooker; Gary High-speed multiple wavelength illumination source, apparatus containing the same, and applications thereof to methods of irradiating luminescent samples and of quantitative luminescence ratio microscopy
JP2909216B2 (ja) 1994-04-29 1999-06-23 パーキン‐エルマー コーポレイション 核酸増幅生成物のリアルタイム検出装置
US5972716A (en) 1994-04-29 1999-10-26 The Perkin-Elmer Corporation Fluorescence monitoring device with textured optical tube and method for reducing background fluorescence
US5766889A (en) * 1994-06-08 1998-06-16 The Perkin-Elmer Corporation Method for determining the characteristics of the concentration growth of target nucleic acid molecules in polymerase chain reaction sample
US5633752A (en) * 1994-07-27 1997-05-27 Olympus Optical Co., Ltd. Optical switching apparatus for a reflected fluorescence microscope
JP3425810B2 (ja) * 1994-07-27 2003-07-14 オリンパス光学工業株式会社 光学系切換装置
US6800452B1 (en) * 1994-08-08 2004-10-05 Science Applications International Corporation Automated methods for simultaneously performing a plurality of signal-based assays
WO1996007888A1 (en) * 1994-09-02 1996-03-14 Biometric Imaging, Inc. Calibration method and apparatus for optical scanner
US5672881A (en) * 1994-09-14 1997-09-30 Glyko, Inc. Charge-coupled device imaging apparatus
AU3963595A (en) * 1994-12-08 1996-06-26 Molecular Dynamics, Inc. Fluorescence imaging system employing a macro scanning objective
DE4444002A1 (de) * 1994-12-10 1996-06-13 Behringwerke Ag Immunoassays für Haptene und hierfür verwendbare Haptentracer-Antikörper-Komplexe sowie Verfahren zur Herstellung derselben
US6014213A (en) * 1994-12-12 2000-01-11 Visible Genetics Inc. High dynamic range apparatus for separation and detection of polynucleotide fragments
US5710628A (en) 1994-12-12 1998-01-20 Visible Genetics Inc. Automated electrophoresis and fluorescence detection apparatus and method
JPH07174701A (ja) 1994-12-28 1995-07-14 Shimadzu Corp 塩基配列決定装置
US5675155A (en) * 1995-04-26 1997-10-07 Beckman Instruments, Inc. Multicapillary fluorescent detection system
US6017434A (en) 1995-05-09 2000-01-25 Curagen Corporation Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
US6485625B1 (en) 1995-05-09 2002-11-26 Curagen Corporation Apparatus and method for the generation, separation, detection, and recognition of biopolymer fragments
US5582705A (en) 1995-05-19 1996-12-10 Iowa State University Research Foundation, Inc. Multiplexed capillary electrophoresis system
US5567947A (en) * 1995-06-01 1996-10-22 Aerodyne Research, Inc. Spectral line discriminator for passive detection of fluorescence
DE19624421B4 (de) * 1995-06-30 2008-07-10 Carl Zeiss Ag Vorrichtung und Verfahren zur ortsaufgelösten Vermessung von Wellenfrontdeformationen
US6411835B1 (en) * 1997-01-13 2002-06-25 Medispectra, Inc. Spectral volume microprobe arrays
JPH0972843A (ja) * 1995-09-06 1997-03-18 Nikon Corp ウェルプレート
US5833827A (en) 1995-09-29 1998-11-10 Hitachi, Ltd. Capillary array electrophoresis system
DE69637086T2 (de) * 1995-10-12 2008-01-10 Lansdorp, Peter M., Vancouver Verfahren zum nachweis von mehrfachkopien einer wiederholungssequenz in einem nukleinsäuremolekül
US5784157A (en) 1995-11-21 1998-07-21 The Research Foundation Of State University Of New York Method and apparatus for identifying fluorophores
JP3152140B2 (ja) * 1995-12-18 2001-04-03 横河電機株式会社 共焦点用光スキャナ
US5926271A (en) * 1995-12-20 1999-07-20 Zeta Technology Laser-induced fluorescence detector having a capillary detection cell and method for identifying trace compounds implemented by the same device
EP0879297A4 (en) * 1995-12-22 2003-04-16 Biorad Lab Inc METHOD AND DEVICE FOR CARRYING OUT FLUORESCENCE IN SITU HYBRIDIZATION MEASURING A VARIETY OF PARAMETERS
US5792610A (en) * 1996-05-01 1998-08-11 Biorad Laboratories, Inc. Method for conducting multiparametric fluorescence in situ hybridization
US5759781A (en) * 1995-12-22 1998-06-02 Yale University Multiparametric fluorescence in situ hybridization
US5863502A (en) * 1996-01-24 1999-01-26 Sarnoff Corporation Parallel reaction cassette and associated devices
US5837196A (en) 1996-01-26 1998-11-17 The Regents Of The University Of California High density array fabrication and readout method for a fiber optic biosensor
US5717602A (en) 1996-02-05 1998-02-10 Kenning; Gregory G. Automated electrophoresis and analysis system
JPH09230246A (ja) * 1996-02-22 1997-09-05 Olympus Optical Co Ltd 走査型光学顕微鏡
JPH09229862A (ja) * 1996-02-28 1997-09-05 Nikon Corp 蛍光測光装置
DE69729659T2 (de) * 1996-02-28 2005-06-23 Johnson, Kenneth C., Santa Clara Mikrolinsen-rastereinrichtung für mikrolithografie und für konfokale mikroskopie mit grossem aufnahmefeld
US5851488A (en) * 1996-02-29 1998-12-22 Biocircuits Corporation Apparatus for automatic electro-optical chemical assay determination
AT403745B (de) 1996-02-29 1998-05-25 Avl Verbrennungskraft Messtech Messanordnung mit einem für anregungs- und messstrahlung transparentem trägerelement
JP2000507350A (ja) * 1996-03-19 2000-06-13 ユニバーシティー オブ ユタ リサーチ ファンデーション 検体の濃度を決定するためのシステム
EP0798559B1 (en) 1996-03-27 2003-09-17 Agilent Technologies, Inc. (a Delaware corporation) Method of detecting sample substances and fluorescence spectrometer using the method
JPH09281078A (ja) 1996-04-09 1997-10-31 Hitachi Electron Eng Co Ltd Ｄｎａ塩基配列決定装置
AU2668697A (en) * 1996-04-12 1997-11-07 Oncor, Inc. Method and composition for controlling formaldehyde fixation by delayed quenching
JPH09292572A (ja) 1996-04-24 1997-11-11 Nikon Corp 落射型蛍光顕微鏡
US5900949A (en) * 1996-05-23 1999-05-04 Hewlett-Packard Company CCD imager for confocal scanning microscopy
NZ333135A (en) * 1996-06-04 2000-02-28 Univ Utah Res Found Systems and methods for monitoring for DNA amplification by fluorescence using acceptor fluorophore labeled nucleic acid probe and a donor fluorophore labeled nucleic acid probe which hybridise to adjacent regions of a target sequence
US5736333A (en) * 1996-06-04 1998-04-07 The Perkin-Elmer Corporation Passive internal references for the detection of nucleic acid amplification products
US5699157A (en) * 1996-07-16 1997-12-16 Caliper Technologies Corp. Fourier detection of species migrating in a microchannel
CA2263226C (en) * 1996-08-16 2006-10-10 Imaging Research, Inc. A digital imaging system for assays in well plates, gels and blots
DE19634873A1 (de) 1996-08-29 1998-03-12 Boehringer Mannheim Gmbh System zur Unterscheidung fluoreszierender Molekülgruppen durch zeitaufgelöste Fluoreszenzmessung
US5754291A (en) * 1996-09-19 1998-05-19 Molecular Dynamics, Inc. Micro-imaging system
JPH1096862A (ja) 1996-09-20 1998-04-14 Bunshi Bio Photonics Kenkyusho:Kk 落射蛍光顕微鏡装置
AU4607197A (en) 1996-09-26 1998-04-17 Sarnoff Corporation Massively parallel detection
US5872623A (en) * 1996-09-26 1999-02-16 Sarnoff Corporation Massively parallel detection
US5854684A (en) * 1996-09-26 1998-12-29 Sarnoff Corporation Massively parallel detection
WO1998017992A2 (en) * 1996-10-25 1998-04-30 Applied Imaging, Inc. Multifluor-fluorescence in situ hybridization (m-fish) imaging techniques using multiple multiband filters with image registration
DE19649605A1 (de) * 1996-11-29 1998-06-04 Deutsches Krebsforsch Fluoreszenzkorrelationsspektroskopiemodul für ein Mikroskop
DE19651667C2 (de) * 1996-12-12 2003-07-03 Rudolf Groskopf Vorrichtung zur dreidimensionalen Untersuchung eines Objektes
US5833826A (en) 1996-12-13 1998-11-10 The Perkin-Elmer Corporation Method and apparatus for reducing the distortion of a sample zone eluting from a capillary electrophoresis capillary
WO1998028075A1 (en) * 1996-12-20 1998-07-02 Imaging Research Inc. A micro-well plate for imaging of fluorescent, chemiluminescent, bioluminescent, and colorimetric assays
EP1249705A3 (en) * 1996-12-31 2003-11-05 Genometrix Genomics Incorporated Multiplexed molecular analysis apparatus and its fabrication method
US5790727A (en) 1997-02-05 1998-08-04 Brookhaven Science Associates Llc Laser illumination of multiple capillaries that form a waveguide
CA2410688C (en) * 1997-02-27 2004-10-26 Cellomics, Inc. A system for cell-based screening
US5958349A (en) * 1997-02-28 1999-09-28 Cepheid Reaction vessel for heat-exchanging chemical processes
US6084667A (en) 1997-03-12 2000-07-04 Nz Applied Technologies System and method for molecular sample measurement
AU7103598A (en) * 1997-04-23 1998-11-13 Packard Instrument Company Inc. Measurement of fluorescence
US6406845B1 (en) * 1997-05-05 2002-06-18 Trustees Of Tuft College Fiber optic biosensor for selectively detecting oligonucleotide species in a mixed fluid sample
BR9816343B1 (pt) * 1997-05-23 2012-08-21 método de operação de um aparelho de teste microbiológico diagnóstico e método de execução de teste microbiológico diagnóstico.
US6365024B1 (en) * 1997-06-30 2002-04-02 Spectrumedix Corporation Motorized positioning apparatus having coaxial carrousels
US5943129A (en) * 1997-08-07 1999-08-24 Cambridge Research & Instrumentation Inc. Fluorescence imaging system
US5834203A (en) 1997-08-25 1998-11-10 Applied Spectral Imaging Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardwire therefore
US5858653A (en) 1997-09-30 1999-01-12 Surmodics, Inc. Reagent and method for attaching target molecules to a surface
DE19748211A1 (de) * 1997-10-31 1999-05-06 Zeiss Carl Fa Optisches Array-System und Reader für Mikrotiterplatten
JP3148702B2 (ja) * 1997-11-07 2001-03-26 オリンパス光学工業株式会社 顕微鏡用落射照明装置
JP3012825B2 (ja) * 1997-11-18 2000-02-28 オリンパス光学工業株式会社 光学顕微鏡
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
JPH11218690A (ja) * 1998-02-04 1999-08-10 Olympus Optical Co Ltd 落射蛍光照明光学系を備えた顕微鏡
US6929953B1 (en) * 1998-03-07 2005-08-16 Robert A. Levine Apparatus for analyzing biologic fluids
US6198577B1 (en) 1998-03-10 2001-03-06 Glaxo Wellcome, Inc. Doubly telecentric lens and imaging system for multiwell plates
US6388788B1 (en) * 1998-03-16 2002-05-14 Praelux, Inc. Method and apparatus for screening chemical compounds
WO1999047922A2 (en) * 1998-03-18 1999-09-23 Massachusetts Institute Of Technology Vascularized perfused microtissue/micro-organ arrays
US6501091B1 (en) 1998-04-01 2002-12-31 Massachusetts Institute Of Technology Quantum dot white and colored light emitting diodes
ATE425448T1 (de) 1998-05-16 2009-03-15 Applera Corp Optisches gerät insbesondere zur überwachung von dns-polymerasekettenreaktionen
US6818437B1 (en) 1998-05-16 2004-11-16 Applera Corporation Instrument for monitoring polymerase chain reaction of DNA
US7498164B2 (en) 1998-05-16 2009-03-03 Applied Biosystems, Llc Instrument for monitoring nucleic acid sequence amplification reaction
US6010756A (en) 1998-05-19 2000-01-04 Lockheed Martin Corporation Rugate filter and method of making same
AT410718B (de) 1998-10-28 2003-07-25 Schindler Hansgeorg Dr Vorrichtung zur visualisierung von molekülen
US6455861B1 (en) * 1998-11-24 2002-09-24 Cambridge Research & Instrumentation, Inc. Fluorescence polarization assay system and method
US6464852B1 (en) 1998-12-03 2002-10-15 State University Of New York At Stony Brook Multicapillary bundle for electrophoresis and detection for DNA
US7115365B2 (en) * 1998-12-07 2006-10-03 Olympus Optical Co., Ltd. Method of analyzing a target nucleic acid
US6110683A (en) 1999-01-08 2000-08-29 Commonwealth Biotechnologies, Inc. Automated DNA Sequencer loading dye which contains a lane tracking aid
US20010006416A1 (en) 1999-01-11 2001-07-05 Johnson Paul E. Ribbon flow cytometry apparatus and methods
US6229252B1 (en) * 1999-01-21 2001-05-08 Asahi Glass Company, Limited Dye combinations for multiple bandpass filters for video displays
US6249341B1 (en) 1999-01-25 2001-06-19 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
US6355934B1 (en) 1999-02-26 2002-03-12 Packard Biochip Technologies Imaging system for an optical scanner
DE19914279C1 (de) 1999-03-25 2000-09-07 Jena Optronik Gmbh Anordnung zum optischen Auslesen der Information von einem matrixförmigen Substrat mit einer Vielzahl von Einzelproben
US7410793B2 (en) 1999-05-17 2008-08-12 Applera Corporation Optical instrument including excitation source
US20050279949A1 (en) 1999-05-17 2005-12-22 Applera Corporation Temperature control for light-emitting diode stabilization
US7387891B2 (en) 1999-05-17 2008-06-17 Applera Corporation Optical instrument including excitation source
AU6075100A (en) * 1999-07-07 2001-01-30 Ljl Biosystems, Inc. Light detection device
US6717668B2 (en) 2000-03-07 2004-04-06 Chemimage Corporation Simultaneous imaging and spectroscopy apparatus
US6381025B1 (en) 1999-08-19 2002-04-30 Texas Tech University Interferometric detection system and method
US6788414B1 (en) 1999-09-09 2004-09-07 Iowa State University Research Foundation, Inc. Method of analyzing multiple sample simultaneously by detecting absorption and systems for use in such a method
WO2001035079A1 (en) 1999-11-12 2001-05-17 E. I. Du Pont De Nemours And Company Fluorometer with low heat-generating light source
US6331438B1 (en) * 1999-11-24 2001-12-18 Iowa State University Research Foundation, Inc. Optical sensors and multisensor arrays containing thin film electroluminescent devices
US20010033376A1 (en) * 2000-02-25 2001-10-25 Cambridge Reseach & Instrumentation Inc. Automatic G-factor calibration
US6749735B1 (en) 2000-03-16 2004-06-15 David Le Febre Electromobility focusing controlled channel electrophoresis system
EP1328894A4 (en) 2000-08-25 2005-11-09 Amnis Corp MEASURING THE SPEED OF SMALL MOBILE OBJECTS SUCH AS CELLS
US7294503B2 (en) 2000-09-15 2007-11-13 California Institute Of Technology Microfabricated crossflow devices and methods
ES2392395T3 (es) 2001-03-01 2012-12-10 Sicpa Holding Sa Detector mejorado de características de luminiscencia
US6529275B2 (en) * 2001-06-22 2003-03-04 Biocal Technology, Inc. Optical detection in bio-separation device using a widened detection zone
US7265833B2 (en) 2001-07-25 2007-09-04 Applera Corporation Electrophoretic system with multi-notch filter and laser excitation source
US6856390B2 (en) 2001-07-25 2005-02-15 Applera Corporation Time-delay integration in electrophoretic detection systems
JP2007163397A (ja) 2005-12-16 2007-06-28 Jtekt Corp 着磁パルサリング
JP2009072843A (ja) 2007-09-19 2009-04-09 Jtekt Corp 研削盤及び研削盤における回転砥石の成形方法
JP4820835B2 (ja) 2008-03-19 2011-11-24 アイシン・エィ・ダブリュ株式会社 運転状態警告システム、運転状態警告方法及びプログラム
EP3567272B1 (en) 2011-09-12 2021-05-26 Fox Factory, Inc. Methods and apparatus for suspension set up

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107044886A (zh) * 2017-01-20 2017-08-15 武汉呵尔医疗科技发展有限公司 一种多波段光谱成像光源、成像方法及显微镜

Also Published As

Publication number Publication date
EP3093649A2 (en) 2016-11-16
ATE403856T1 (de) 2008-08-15
JP2013178254A (ja) 2013-09-09
EP2315002A3 (en) 2012-12-05
JP2016195602A (ja) 2016-11-24
JP2005274579A (ja) 2005-10-06
AU759974B2 (en) 2003-05-01
EP3093649A3 (en) 2017-04-26
EP1978351A2 (en) 2008-10-08
CN1309766A (zh) 2001-08-22
EP2315002A2 (en) 2011-04-27
JP2011039071A (ja) 2011-02-24
DE69940574D1 (de) 2009-04-23
WO1999060381A1 (en) 1999-11-25
US9671342B2 (en) 2017-06-06
ATE425448T1 (de) 2009-03-15
US20070238161A1 (en) 2007-10-11
EP1078245B1 (en) 2008-08-06
EP1978351B1 (en) 2011-11-30
AU4088499A (en) 1999-12-06
CN1664562A (zh) 2005-09-07
JP2015072285A (ja) 2015-04-16
DE69939259D1 (de) 2008-09-18
JP2009002955A (ja) 2009-01-08
JP2003524754A (ja) 2003-08-19
EP1978351A3 (en) 2008-10-15
CA2328609A1 (en) 1999-11-25
EP1078245A1 (en) 2001-02-28
EP3093649B1 (en) 2019-05-08
JP5881226B2 (ja) 2016-03-09

Similar Documents

Publication Publication Date Title
CN1201016C (zh) 用于监测dna聚合酶链反应的仪器
US9273353B2 (en) Instrument for monitoring polymerase chain reaction of DNA
US8361785B2 (en) Optical instrument comprising multi-notch beam splitter
US7928408B2 (en) Multi-channel fluorescence measuring optical system and multi-channel fluorescence sample analyzer
JP5174035B2 (ja) 二次元配列画像化
JP2005526254A (ja) 励起光源を備える光学機器
EP1619491B1 (en) Optical instrument, particulary for monitoring DNA polymerase chain reactions
CA2566924C (en) Instrument for monitoring polymerase chain reaction of dna

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
ASS Succession or assignment of patent right

Owner name: APPLERLA CO.,LTD.

Free format text: FORMER OWNER: THE PERKIN-ELMER CORP.

Effective date: 20050610

C41 Transfer of patent application or patent right or utility model
TR01 Transfer of patent right

Effective date of registration: 20050610

Address after: The United States Delaware

Patentee after: Applera Corp.

Address before: American Connecticut

Patentee before: The Perkin-Elmer Corp.

CX01 Expiry of patent term

Granted publication date: 20050511

CX01 Expiry of patent term