CN108699566A - 对病毒动力学影响最小的治疗性腺病毒中的外源基因表达 - Google Patents

对病毒动力学影响最小的治疗性腺病毒中的外源基因表达 Download PDF

Info

Publication number
CN108699566A
CN108699566A CN201780013061.3A CN201780013061A CN108699566A CN 108699566 A CN108699566 A CN 108699566A CN 201780013061 A CN201780013061 A CN 201780013061A CN 108699566 A CN108699566 A CN 108699566A
Authority
CN
China
Prior art keywords
orf
recombined adhenovirus
virus
genome
subject
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201780013061.3A
Other languages
English (en)
Other versions
CN108699566B (zh
Inventor
C·欧谢伊
W·帕特洛
C·鲍尔斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Salk Institute for Biological Studies
Original Assignee
Salk Institute for Biological Studies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Salk Institute for Biological Studies filed Critical Salk Institute for Biological Studies
Priority to CN202310696656.0A priority Critical patent/CN117384961A/zh
Publication of CN108699566A publication Critical patent/CN108699566A/zh
Application granted granted Critical
Publication of CN108699566B publication Critical patent/CN108699566B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0008—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/04—Antineoplastic agents specific for metastasis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10021—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10041—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Oncology (AREA)
  • Epidemiology (AREA)
  • Hematology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

本发明描述了包括外源开放阅读框(ORF)和自切割肽编码序列的重组腺病毒基因组。进一步公开了对病毒动力学影响最小的外源基因的最佳放置。还描述了所述重组腺病毒的治疗性应用。

Description

对病毒动力学影响最小的治疗性腺病毒中的外源基因表达
相关申请的交叉引用
本申请要求2016年2月23日提交的美国临时申请No.62/298,653的权益，通过引用将其全部并入本文
发明领域
本公开涉及在重组腺病毒构建体中外源开放阅读框的最佳放置以及该重组病毒的治疗性应用。
背景技术
腺病毒血清型5(Ad5)是在基础研究应用、鼠肺癌模型和人类基因治疗试验中选择的载体。腺病毒具有稳定的36kb双链DNA基因组，其由Ad纤维蛋白刺突(spikes)装饰的蛋白衣壳保护，所述Ad纤维蛋白刺突(spikes)靶向感染特定细胞类型上的受体。腺病毒不整合到宿主DNA中，可以使用已建立的方案将其生产至高滴度，并且在人类基因治疗和癌症应用中已经证实安全性。因此，基于Ad的载体对癌症诊断和治疗具有巨大的希望。
发明内容
本文公开包括异源开放阅读框(ORF)和自切割肽编码序列的重组腺病毒基因组。所述异源ORF可编码，例如治疗性蛋白。由所述公开的基因组产生的所述重组腺病毒基因组和重组腺病毒可用于，例如治疗性应用，诸如用于癌症的治疗。
本文提供重组腺病毒基因组，其包括异源ORF和自切割肽编码序列，两者均可操作地连接至与内源腺病毒ORF相同的阅读框中且在所述相同的阅读框中。所述自切割肽编码序列位于所述异源ORF和所述内源ORF之间。在一些实施方案中，所述内源ORF是E1B-55k且所述异源ORF在E1B-55k的3'；所述内源ORF是DNA聚合酶且所述异源ORF在DNA聚合酶的5'；所述内源ORF是DNA结合蛋白(DBP)且所述异源ORF在DBP的3'；所述内源ORF是腺病毒死亡蛋白(ADP)且所述异源ORF在ADP的5'；所述内源ORF是E3-14.7k且所述异源ORF在E3-14.7k的3'；所述内源ORF是E4-ORF2且所述异源ORF在E4-ORF2的5'；或者所述内源ORF是纤维蛋白且所述异源ORF在纤维蛋白的3'。在一些实例中，所述异源ORF编码治疗性蛋白。
本文进一步提供包括本文公开的重组腺病毒基因组的重组腺病毒。还提供包括本文公开的重组腺病毒基因组或重组腺病毒和药学上可接受的载体的组合物。
还提供通过向所述受试者施用本文公开的重组腺病毒基因组或重组腺病毒(或其组合物)将治疗性蛋白递送至受试者的方法。在这些方法中，所述重组腺病毒基因组的异源ORF或重组腺病毒的所述异源ORF编码所述治疗性蛋白。
进一步提供了通过施用本文公开的重组腺病毒基因组或重组腺病毒(或其组合物)抑制肿瘤细胞活力、抑制肿瘤细胞生长、抑制肿瘤进展、减小肿瘤体积以及治疗患有癌症的受试者的方法。在这些方法中，所述重组腺病毒基因组或重组腺病毒的所述异源ORF编码适合癌症治疗的治疗性蛋白。
根据以下参照附图进行的详细描述，本公开的前述和其他客体和特征将变得更加明显。
附图简要说明
图1是用于测试腺病毒构建体的示例性工作流程的示意图。产生全病毒基因组质粒并将其转染到多孔板中合适的细胞中，例如293-E4细胞。当转染的细胞扩增(expand)，它们进行冷冻/解冻以释放病毒粒子，然后离心以沉淀细胞碎片。将上清液(含有所述病毒粒子)转移到多个、较大的培养板中。从转染的细胞中收获病毒粒子，CsCl纯化的(CsClpurified)和感染性病毒滴度通过ELISA测量。然后用纯化的病毒的已知的MOI感染感兴趣的细胞类型。在感染后48或72小时，分别通过Western印迹、q-PCR或噬斑试验来测量腺病毒晚期蛋白、腺病毒基因组或噬斑。
图2是显示指数病毒生长的示意图。溶瘤杀死肿瘤内的所有细胞需要指数病毒生长。然而，在大多数情况下，最初仅感染小百分比的肿瘤细胞。因此，每轮复制的后代数量的小差异导致仅几轮复制后粒子总数的大差异。所示为每个循环产生3个病毒粒子的病毒与每个循环产生5个病毒粒子的病毒之间的比较。如图所示，在5-6轮复制后，两种病毒的病毒滴度显著不同。
图3是显示基于荧光的病毒动力学(FBVK)测定的示例性工作流程的示意图。(例如通过Adsembly或AdSLIC)产生全病毒基因组质粒并将其用于转染多孔板中感兴趣的细胞类型。作为选择，用重组腺病毒粒子感染细胞。所述腺病毒基因组包括至少一个位于所述病毒基因组中编码荧光蛋白的开放阅读框(ORF)，其基本上不改变病毒复制动力学。随时间监测荧光以计算病毒复制动力学。溶瘤病毒候选物是在肿瘤细胞和正常细胞之间表现出最大的病毒动力学差异的那些。
图4A-4B概述当用腺病毒基因组质粒开始时的动力学测定设置。该测定不需要准确知道最初的转染效率。选择转染条件以导致最初约5-10％的细胞被转染。在所示的实施例中，使用48孔板，其允许一式三份地测试14种不同的病毒构建体，连同三个模拟感染的孔和三个具有FluoresbriteTM珠子的孔以补偿工具灵敏度漂移。(图4A)所述48孔板的上半部分的孔含有用6种不同病毒的基因组质粒转染的细胞，模拟感染的细胞和空白(FluoresbriteTM珠子)，每种一式三份。(图4B)所述48孔板下半部分的孔含有用8种不同病毒的基因组质粒转染的细胞，一式三份。将所述多孔板置于读板仪(诸如TECAN读板仪)上以进行连续的荧光监测。
图5概述当用重组病毒开始时的动力学测定设置。该测定不需要准确知道病毒滴度。将重组病毒连续稀释并将其用于感染铺在多孔板中的细胞。在所示的实例中，使用96孔板，每种病毒以1:100、1:300、1:900、1:2700、1:8100、1:24,300、1:72,900和1:218,700稀释，允许同时测试11种病毒。模拟感染四个孔并将FluoresbriteTM珠置于四个孔中以补偿工具灵敏度和漂移。将所述多孔板置于读板仪(例如TECAN读板仪)上以进行连续的荧光监测。
图6A-6C提供用于组合装配重组腺病毒的Adsembly和AdSLIC技术的示意概览。(图6A)将所述腺病毒基因组分成四个模块-E1、核心、E3和E4。(图6B)Adsembly涉及使用多位点通道反应的基因组重新组装。(图6C)AdSLIC利用序列和非连接依赖性克隆(SLIC)来组装腺病毒模块。
图7是显示在所述E1区中编码荧光蛋白的重组腺病毒的ln-斜率值的柱状图。显示直接融合构建体YPet-E1A，和YPet-P2A-E1A、E1A-P2A-mCherry和E1B-55k-P2A-YPet构建体的值，其各自含有P2A位点。显示所述YPet-P2A-ADP构建体用于比较。
图8是基于荧光的病毒动力测定的动力学数据分析和解释的示意图。
图9A-9C是显示来源于Ad5、Ad9或Ad34并含有在E3-14.7k ORF(或其在Ad9和Ad34中的等同物)的3'的异源ORF的重组腺病毒的ln-斜率值的柱状图。显示的是Ad5(E3-14.7k-P2A-YPet；PCMN-887)、Ad9(E3-15k-P2A-YPet；PCMN-888)和Ad34(E3-14.8k-P2A-YPet；PCMN-889)在293细胞中(图9A)、A549细胞(图9B)和U2OS细胞(图9C)中的值。每个图中还显示了包括Ad5核心(其包括E3-14.7k-P2A-YPet)和来自Ad9(Ad5/Ad9)或Ad34(Ad5/Ad34)的纤维轴/球突(fiber shaft/knob)的嵌合病毒的值。
序列表
列在所附序列表中的核酸和氨基酸序列采用针对核苷酸碱基的标准字母缩写和针对氨基酸的三字母代码显示，如37C.F.R.1.822中所定义的。仅显示了每条核酸序列的一条链，但互补链被理解为包括在对所显示链的任何引用中。该序列表作为ASCII文本文件提交，其于2017年2月21日生成的，703KB，其通过引用并入本文。在所附的序列表中：
SEQ ID NO:1是合成腺病毒基因组CMBT-379(YPet-P2A-E1A)的核苷酸序列。
SEQ ID NO:2是合成腺病毒基因组CMBT-432(E1A-P2A-YPet)的核苷酸序列。
SEQ ID NO:3是合成腺病毒基因组CMBT-456(E1B-55k-P2A-YPet)的核苷酸序列。
SEQ ID NO:4是合成腺病毒基因组CMBT-499(E1B-55k-P2A-mCherry)的核苷酸序列。
SEQ ID NO:5是合成腺病毒基因组CMBT-530(YPet-P2A-(DNA Poly))的核苷酸序列。
SEQ ID NO:6是合成腺病毒基因组CMBT-886(DBP-P2A-YPet)的核苷酸序列。
SEQ ID NO:7是合成腺病毒基因组CMBT-403(YPet-P2A-ADP)的核苷酸序列。
SEQ ID NO:8是合成腺病毒基因组CMBT-429(ADP-P2A-YPet)的核苷酸序列。
SEQ ID NO:9是合成腺病毒基因组PCMN-887(E3-14.7k-P2A-YPet)的核苷酸序列。
SEQ ID NO:10是合成腺病毒基因组CMBT-457(YPet-P2A-E4-ORF2)的核苷酸序列。
SEQ ID NO:11是合成腺病毒基因组CMBT-633(mCherry-P2A-E4-ORF2)的核苷酸序列。
SEQ ID NO:12是合成腺病毒基因组CMBT-407(YPet-P2A-纤维蛋白)的核苷酸序列。
SEQ ID NO:13是合成腺病毒基因组CMBT-445(纤维蛋白-P2A-YPet)的核苷酸序列。
SEQ ID NO:14是P2A的氨基酸序列。
SEQ ID NO:15是F2A的氨基酸序列。
SEQ ID NO:16是E2A的氨基酸序列。
SEQ ID NO:17是T2A的氨基酸序列。
SEQ ID NO:18是在N末端包括GSG的经修饰的P2A的氨基酸序列。
SEQ ID NO:19是在N末端包括GSG的经修饰的F2A的氨基酸序列。
SEQ ID NO:20是在N末端包括GSG的经修饰的E2A的氨基酸序列。
SEQ ID NO:21是在N末端包括GSG的经修饰的T2A的氨基酸序列。
SEQ ID NO:22是合成腺病毒基因组PCMN-888(Ad9E3-15k-P2A-YPet)的核苷酸序列。
SEQ ID NO:23是合成腺病毒基因组PCMN-889(Ad34E3-14.8k-P2A-YPet)的核苷酸序列。
发明详述
I.缩写
Ad 腺病毒
ADP 腺病毒死亡蛋白
BFP 蓝色荧光蛋白
E2A 马鼻炎A病毒2A
ELISA 酶联免疫吸附测定
ERAV 马鼻炎A病毒
F2A 口蹄疫病毒2A
FACS 荧光激活细胞分选
FMDV 口蹄疫病毒
GFP 绿色荧光蛋白
MOI 感染的多样性
OD 光密度
ORF 开放阅读框
P2A 猪捷申病毒-1 2A(porcine teschovirus-1 2A)
pIX 蛋白IX
PTV1 猪捷申病毒-1
RFP 红色荧光蛋白
SLIC 序列和连接非依赖性克隆
T2A 明脉扁刺蛾β四体病毒2A(Thosea asigna virus 2A)
TaV 明脉扁刺蛾β四体病毒(Thosea asigna virus)
YFP 黄色荧光蛋白
II.术语和方法
除非另有说明，否则技术术语按常规用法使用。对分子生物学中常见术语的定义可以在牛津大学出版社出版的Benjamin Lewin,Genes V,1994(ISBN 0-19-854287-9)；Blackwell Science Ltd.出版的Kendrew等(eds.),The Encyclopedia of MolecularBiology,1994(ISBN 0-632-02182-9)；以及VCH Publishers,Inc.出版的Robert A.Meyers(ed.),Molecular Biology and Biotechnology:a Comprehensive Desk Reference,1995(ISBN 1-56081-569-8)中找到。
为了方便查看本公开的各种实施方案，提供了以下对具体术语的解释：
2A多肽：一种由一些RNA病毒编码的自切割肽的类型，例如小核糖核酸病毒。2A肽通过使核糖体跳过结合在2A元件的C末端的肽的合成而起作用，导致所述2A序列末端与下游肽之间的分离(Kim等，PLoS One 6(4):e18556,2011)。所述“切割”发生在所述2A肽的C末端上发现的甘氨酸和脯氨酸残基之间。示例性的2A肽包括但不限于，由明脉扁刺蛾β四体病毒(TaV)、马鼻炎A病毒(ERAV)、猪捷申病毒-1(PTV1)和口蹄疫病毒(FMDV)编码的所述2A肽，它们在本文中如SEQ ID NO:14-17所示。在一些实施方案中，所述2A肽在N-末端包括Gly-Ser-Gly以提高切割效率(SEQ ID NO:18-21)。
腺病毒：具有线性、双链DNA基因组和二十面体衣壳的无包膜病毒。目前有68种已知的人腺病毒血清型，其分为7种(种A、B、C、D、E、F和G)。腺病毒的不同血清型与不同类型的疾病相关，一些血清型引起呼吸道疾病(主要是种B和C)、结膜炎(种B和D)和/或胃肠炎(种F和G)。
腺病毒死亡蛋白(ADP)：在腺病毒感染晚期合成的蛋白，其介导细胞分裂并释放腺病毒以感染其他细胞。ADP是定位于核膜、内质网和高尔基体的101个氨基酸的完整膜糖蛋白。ADP以前称为E3-11.6K。
施用：将制剂(诸如治疗剂(例如重组病毒))通过任何有效的途径提供或给受试者。示例性施用途径包括，但不限于，注射(诸如皮下、肌内、皮内、腹膜内、肿瘤内和静脉内)，口服、导管内、舌下、直肠、透皮、鼻内、阴道和吸入途径。
嵌合：由具有不同起源的至少两个部分组成。在本公开的上下文中，“嵌合腺病毒”是具有来源于至少两个不同血清型的遗传物质和/或蛋白质的腺病毒(诸如，来源于Ad5和腺病毒的第二血清型)。在这个背景下，“衣壳-交换的(capsid-swapped)”腺病毒指嵌合腺病毒，在其中所述衣壳蛋白来源于腺病毒的一种血清型，且剩下的蛋白来源于另一种腺病毒血清型。相似地，“嵌合纤维(chimeric fiber)”是具有来源于至少两种不同的腺病毒血清型的氨基酸序列的纤维蛋白。例如，嵌合纤维可由来自Ad5的纤维轴和来自腺病毒的第二血清型的纤维球突组成。在另一个实例中，嵌合纤维由Ad5尾巴和来自腺病毒的第二血清型(诸如Ad9或Ad34)的纤维轴(fiber shaft)和球突(knob)组成。
接触：置于直接的物理联系中；包括在固体和液体形式中。
简并变体：在本公开上下文中，“简并变体”指编码蛋白的多核苷酸，其包括作为遗传密码的结果而简并的(degenerate)的序列。存在20种天然氨基酸，其大多数由多于一个的密码子指定(specified)。因此，包括编码肽的所有简并核苷酸序列，只要由所述核苷酸序列编码的所述肽的氨基酸序列不变。
被删除的(deleted)：编码“被删除的”蛋白质的腺病毒基因组(诸如E4orf1或E4orf6/7蛋白)指具有导致蛋白表达缺失的所述蛋白编码序列的完全删除或部分删除的腺病毒。
E2F的失调(deregulation)：指所述E2F转录因子和下游靶基因的活性增加，其发生在几乎所有类型的人类癌症中。E2F途径活性和转录的失调可由所述途径的任何上游组分中的多种不同突变引起，例如Rb、p107和p130肿瘤抑制因子的功能丧失突变和缺失。Rb是第一个被鉴定的肿瘤抑制因子，并且在至少三分之一的人类肿瘤中不存在或突变。此外，p16突变和/或表观遗传沉默可激活肿瘤细胞中的E2F。细胞周期蛋白D(Cyclin D)和CDK4突变、基因扩增或过表达也可导致对人肿瘤中的E2F活性的失调。此外，E2F由生长因子受体途径突变激活，包括EGFR、RTK、RAS、RAF、PI-3K、PTEN、RAF、MYC。p16INK4a-Cyclin D:cdk4/6-RB-E2F途径中的突变通常以相互排斥的方式发生，因此一个‘命中(hit)’(例如，p16)不与其他的命中(例如，Rb突变或细胞周期蛋白D:cdk过表达)相伴随。然而，大多数目前的化学疗法是抑制E2F转录靶标的增殖性毒物，但也对正常细胞有毒并且具有经常是毁灭性的医源性并发症。如本文所公开的，一种可选择的治疗方法是使用在癌细胞损伤中经历选择性裂解性复制的病毒，所述癌细胞损伤具有失调的p16-细胞周期蛋白D:cdk4-RB-E2F途径。
DNA-结合蛋白(DBP)：该腺病毒蛋白结合至单链DNA和RNA，以及双链DNA。一种72-千道尔顿蛋白DBP对腺病毒DNA的复制是必不可少的。
E1A：腺病毒早期区域1A(E1A)基因和表达自所述基因的多肽。所述E1A蛋白在病毒基因组复制中通过操纵细胞进入细胞周期而起作用。如本文中所使用的，术语“E1A蛋白”指表达自所述E1A基因的蛋白质，且所述术语包括由任何腺病毒血清型产生的E1A蛋白。
E3-RIDα/RIDβ和E3-14.7k：产自所述E3基因的早期表达蛋白。所述E3-RIDα、E3-RIDβ和E3-14.7k蛋白构成受体内化和降解复合物(RID)，其定位于核膜并引起包括CD95(FasL受体)以及TNFR1和2(TNF/TRAIL受体)的多种受体的内吞作用和降解，以保护受感染的细胞免受宿主抗病毒的应答。所述E3-RIDα、E3-RIDβ和E3-14.7k编码序列按此顺序彼此相邻。
E4orf1：产自所述E4基因的腺病毒蛋白。术语“E4orf蛋白”包括由来自任何一种腺病毒血清型的所述E4基因产生的E4orf1蛋白。
E4orf6/7：由所述腺病毒E4基因编码的蛋白。术语“E4orf6/7蛋白”包括由来自任何一种腺病毒血清型的所述E4基因产生的E4orf6/7蛋白。
纤维(fiber)：所述腺病毒纤维蛋白是介导结合至细胞表面受体的嵌合蛋白。所述纤维蛋白由长N末端轴和球状C末端球突组成。
荧光蛋白：当暴露于具体波长的光时，发射某种波长的光的蛋白质。荧光蛋白包括，但不限于，绿色荧光蛋白(诸如GFP、EGFP、AcGFP1、祖母绿、超折叠GFP、Azami绿(AzamiGreen)、m芥末绿(mWasabi)、标签GFP(TagGFP)、TurboGFP和Zs绿(ZsGreen))，蓝色荧光蛋白(诸如EBFP、EBFP2、天蓝色(Sapphire)、T-天蓝色(T-Sapphire)、蓝铜蓝(Azurite)和m标签BFP(mTagBFP))，蓝绿色荧光蛋白(诸如ECFP、mECFP、蔚蓝色(Cerulean)、CyPet、Am蓝绿色1(AmCyan1)、Midori-Ishi蓝绿色(Midori-Ishi Cyan)、m绿松石蓝绿色(mTurquoise)和mTFP1)，黄色荧光蛋白(EYFP、黄晶黄(Topaz)、金星黄(Venus)、m黄水晶黄(mCitrine)、YPet、标签YFP(TagYFP)、PhiYFP、Zs黄1(ZsYellow1)和m香蕉黄(mBanana))，橙色荧光蛋白(Kusabira橙(Kusabira Orange)、Kusabira橙2(Kusabira Orange2)、m橙(mOrange)、m橙2(mOrange2)和m橘红(mTangerine))，红色荧光蛋白(m红宝石红(mRuby)、m苹果红(mApple)、m草莓红(mStrawberry)、As红2(AsRed2)、mRFP1、J红(JRed)、m樱桃红(mCherry)、Hc红1(HcRed1)、m覆盆子红(mRaspberry)、d桂马红-串联(dKeima-Tandem)、Hc红-串联(HcRed-Tandem)、m紫红(mPlum)、AQ143、td番茄红(tdTomato)和E2-深红(E2-Crimson))，橘色/红色荧光蛋白(d番茄红(dTomato)、d番茄红-串联(dTomato-Tandem)、标签RFP(TagRFP)、标签RFP-T(TagRFP-T)、Ds红(DsRed)、Ds红2(DsRed2)、Ds红-表达(T1)(DsRed-Express(T1))和Ds红-单体(DsRed-Monomer))及其经修饰的版本。
融合蛋白：含有来自至少两种不同(异源)蛋白或肽的氨基酸序列的蛋白。融合蛋白可例如通过核酸序列的表达生成，所述核酸序列从编码两种不同(异源)蛋白的至少一部分的核酸序列的工程化而来。为了生成融合蛋白，所述核酸序列必须在相同的阅读框中且不含有内部的终止密码子。融合蛋白，特别是短融合蛋白，也可由化学合成生成。
异源：异源蛋白或多肽指来源于不同来源或物种的蛋白质或多肽。
六邻体(Hexon)：主要的腺病毒衣壳蛋白。
免疫调节剂：改变(例如，活化、提高或抑制)所述免疫系统的试剂(agent)。免疫调节剂包括，但不限于，细胞因子(诸如白细胞介素2(IL-2)、IL-7、IL-12、GM-CSF、FLT3配体或干扰素)、趋化因子(诸如CCL3、CCL26、CXCL7、CXCL9和CXCL10)，T细胞激活配体(诸如抗CD3Abs或同种异体抗原(alloantigen))、共刺激分子(诸如B7.1/B7.2、OX40L、4-1-BBL或CD40L)、检查点阻塞抑制剂(checkpoint blockade inhibitors)(诸如抗PD-1或抗CTLA4Abs)、和小分子免疫调节剂。
分离的(isolated)：已经将“分离的”生物学组分(诸如核酸分子、蛋白、病毒或细胞)从生物体的细胞或组织中或生物体本身中的其他生物组分中基本上分开或纯化，在其中所述组分是天然发生的，诸如其他染色体的和染色体外的DNA和RNA、蛋白质和细胞。已经被“分离的”核酸分子和蛋白质包括通过标准纯化方法纯化的那些。所述术语还包括通过在宿主细胞中重组表达制备的核酸分子和蛋白质，以及化学合成的核酸分子和蛋白质。
修饰：核酸序列或蛋白质序列上的改变。例如，氨基酸序列修饰包括，例如，取代、插入和缺失、或其组合。插入包括氨基和/或羧基末端融合以及单个或多个氨基酸残基的序列内插入。缺失的特征在于从所述蛋白质序列中除去一个或更多个氨基酸残基。在本文的一些实施方案中，所述修饰(诸如取代、插入或缺失)导致功能的改变，诸如降低或加强蛋白质的具体活性。如本文所使用的，“Δ”或“delta”指缺失。取代修饰是在其中至少一个残基已被除去且将与之不同的残基插入到它的位置的那些。氨基酸取代通常是单个残基，但一次可发生在许多不同的位置。因此可以组合取代、缺失、插入或其任何组合以获得最终的突变序列。这些修饰可以通过修饰所述编码蛋白质的DNA中的核苷酸来制备，从而产生编码该修饰的DNA。在具有已知序列的DNA中的预定位点处进行插入、缺失和取代突变的技术是本领域熟知的。“经修饰的”蛋白质、核酸或病毒是具有如上概述的一种或更多种修饰的蛋白质、核酸或病毒。
瘤形成(neoplasia)、恶性肿瘤(malignancy)、癌症(cancer)和肿瘤(tumor)：瘤(neoplasm)是由于过度细胞分裂导致的组织或细胞的异常生长。瘤的生长可以产生肿瘤。个体中肿瘤的量是“肿瘤负荷”，其可以作为所述肿瘤的数量、体积或重量来测量。不转移的肿瘤被称为“良性的”。侵入周围组织和/或可转移的肿瘤被称为“恶性的(malignant)”。恶性的肿瘤也被称为“癌症”。
血液系统癌症是血液或骨髓的癌症。血液学(或血源性)癌症的实例包括白血病，其包括急性白血病(例如急性淋巴细胞白血病、急性髓细胞白血病、急性髓性白血病以及成髓细胞的、早幼粒细胞的、髓单核细胞的、单核细胞的和红白血病)、慢性白血病(例如慢性髓细胞(粒细胞))白血病、慢性髓性白血病和慢性淋巴细胞白血病)、真性红细胞增多症、淋巴瘤、霍奇金病(Hodgkin's disease)、非霍奇金淋巴瘤(惰性和高级形式)、多发性骨髓瘤、瓦尔登斯特伦巨球蛋白血症(Waldenstrom's macroglobulinemia)、重链疾病、骨髓发育不良综合征、毛细胞白血病和骨髓发育不良。在某些情况下，淋巴瘤被认为是实体瘤。
实体瘤是通常不含有囊肿或液体区域的异常组织块。实体瘤可以是良性的或恶性的。不同类型的实体瘤以形成它们的细胞类型命名(例如肉瘤，恶性上皮肿瘤和淋巴瘤)。实体瘤的实例，诸如肉瘤和恶性上皮肿瘤，包括纤维肉瘤、粘液肉瘤、脂肪肉瘤、软骨肉瘤、骨肉瘤和其他肉瘤、滑膜瘤、间皮瘤、尤因氏瘤(Ewing's tumor)、平滑肌肉瘤、横纹肌肉瘤、结肠癌、淋巴恶性肿瘤、胰腺癌、乳腺癌、肺癌、卵巢癌、前列腺癌、肝细胞癌、鳞状细胞癌、基底细胞癌、腺癌、汗腺癌、甲状腺髓样癌、乳头状甲状腺癌、嗜铬细胞瘤皮脂腺癌(pheochromocytomas sebaceous gland carcinoma)、乳头状癌、人乳头状瘤病毒(HPV)-感染的瘤形成、乳头状腺癌(papillary adenocarcinomas)、髓样癌、支气管癌、肾细胞癌、肝癌、胆管癌、绒毛膜癌、维尔姆斯瘤(Wilms'tumor)、宫颈癌、睾丸肿瘤、精原细胞瘤、膀胱癌、黑色素瘤和CNS肿瘤(如脑胶质瘤(如脑干胶质瘤和混合胶质瘤)、成胶质细胞瘤(又称多形性胶质母细胞瘤)星形细胞瘤、CNS淋巴瘤、生殖细胞瘤、成神经管细胞瘤、神经鞘瘤颅咽管瘤(Schwannoma craniopharyogioma)、室管膜瘤、松果体瘤、成血管细胞瘤、听神经瘤、少突神经胶质瘤、黑色素瘤(menangioma)、成神经细胞瘤、成视网膜细胞瘤(retinoblastoma)和脑转移瘤)。
溶瘤病毒：选择性杀死增殖性疾病细胞(例如癌/肿瘤细胞)的病毒。可以通过本领域中建立的任何方法检测癌细胞的杀死，例如确定活细胞计数，或检测癌细胞中的细胞病变效应、细胞凋亡或病毒蛋白的合成(例如，通过复制所必需的病毒基因的代谢标记、免疫印迹或RT-PCR)，或肿瘤大小的减小。
可操作地连接：当第一核酸序列与第二核酸序列处于功能关系时，将第一核酸序列与第二核酸序列可操作地连接。例如，如果启动子影响编码序列的转录或表达，则将所述启动子与所述编码序列可操作地连接。通常，可操作地连接的DNA序列是连续的并且，在必要时将两个蛋白质编码区连接在同一阅读框中。
药学上可接受的载体：在本公开中有用的药学上可接受的载体(媒介(vehicles))是常规的。Remington’s Pharmaceutical Sciences,by E.W.Martin,Mack PublishingCo.,Easton,PA,15th Edition(1975)描述了适用于一种或更多种治疗性化合物、分子或制剂(例如本文公开的重组病毒)的制药学递送的组合物和制剂。
通常，所述载体的性质取决于所采用的具体施用模式。例如，胃肠外制剂通常包括可注射流体，所述可注射流体包括作为媒介的药学和生理学上可接受的流体，诸如水、生理盐水、平衡盐溶液、葡萄糖水溶液、甘油等。对于固体组合物(例如，粉末、药丸、片剂或胶囊形式)，常规的无毒固体载体可包括，例如药用级甘露醇、乳糖、淀粉或硬脂酸镁。除了生物学上中性载体，待施用的药物组合物还可含有小量的无毒辅助物质，例如润湿剂或乳化剂、防腐剂以及pH缓冲剂等，例如醋酸钠或脱水山梨醇单月桂酸酯。
多肽、肽或蛋白质：其中单体为氨基酸残基的多聚物，这些氨基酸残基通过酰胺键联结在一起。当所述氨基酸为α氨基酸时，L光学异构体或D光学异构体都可使用。术语“多肽”、“肽”或“蛋白质”在本文中可交换使用。这些术语应用于氨基酸聚合物，其中一种或更多种氨基酸残基是相应地天然存在的氨基酸的人工化学模拟物，以及天然发生的氨基酸聚合物和非天然发生的氨基酸聚合物。术语“残基”或“氨基酸残基”包括提及被并入到蛋白质、多肽或肽中的氨基酸。
多肽中的保守取代是将在蛋白质序列中的一个氨基酸残基取代为具有相似生物化学性质的与之不同氨基酸残基。通常，保守取代对所得多肽的活性的影响很小直至没有影响。例如，包括一个或更多个保守取代(例如不多于1、2、3、4或5个取代)的蛋白质或肽保留所述野生型蛋白质或肽的结构和功能。含有一个或更多个保守取代的多肽可以通过使用例如标准程序(如定点突变或PCR)操纵编码所述多肽的核苷酸序列而产生。在一个实例中，此类变体可以通过测试抗体交叉反应性或其诱导免疫应答的能力来容易地选择。保守取代的实例如下所示。
保守取代通常保持(a)所述取代区域中多肽骨架的结构，例如，作为片状或螺旋构象，(b)分子在靶位点的电荷或疏水性，或(c)侧链的大部分。
通常预期产生蛋白质性质的最大变化的取代将是非保守的，例如其中的变化(a)用亲水性残基(例如，丝氨酰或苏氨酰)取代(或将其取代为)疏水性残基，例如，亮氨酰、异亮氨酰、苯丙氨酰、缬氨酰或丙氨酰；(b)用半胱氨酸或脯氨酸取代(或将其取代为)任何其他残基；(c)用具有正电性侧链的残基，例如赖氨酰基，精氨酰基或组氨酰基，取代(或将其取代为)负电性残基，例如谷氨酰基或天冬氨酰基；或(d)用具有庞大侧链的残基，例如苯丙氨酸，取代(或将其取代为)不具有侧链的残基，例如甘氨酸。
预防、治疗或缓解疾病：“预防”疾病指抑制疾病的完全出现。“治疗”指在疾病或病理状况已开始出现后缓解该疾病或病理状况的体征或症状的治疗性介入。“缓解”指在疾病的体征或症状的数量或严重程度上的降低。
启动子：指导/启动核酸(例如基因)转录的DNA区。启动子包括转录起始位点附近的必需核酸序列。通常，启动子位于它们转录的基因附近。启动子还任选地包括远端增强子或阻遏物元件，其可位于距转录起始位点数千碱基对的位置。“组成型启动子”是连续有活性的且不受外部信号或分子调节的启动子。相反，“诱导型启动子”的活性受外部信号或分子(例如转录因子或四环素)的调节。
蛋白IX(Protein IX(pIX))：与所述六邻体蛋白连接的腺病毒衣壳的次要组分。
纯化的：术语“纯化的”不需要绝对纯净；相反，意欲将它作为一个相对术语。因此，例如，纯化的肽、蛋白质、病毒或其他活性化合物是从天然相关蛋白质和其他污染物中全部或部分地分离的肽、蛋白质、病毒或其他活性化合物。在某些实施方案中，术语“基本上纯化的”是指已经从细胞、细胞培养基或其他未加工的制备物中分离并经过分馏以除去初始制备物的各种组分的肽、蛋白质、病毒或其他活性化合物，如蛋白质、细胞碎片和其他组分。
重组：重组核酸分子、蛋白质或病毒是这样的核酸分子、蛋白质或病毒，其具有非天然发生的序列或具有通过人工组合两个原本分开的序列片段而形成的序列。这种人工组合可通过化学合成或者通过人工操作分离的核酸片段完成，例如通过基因工程技术来完成。术语“重组”还包括单独通过所述天然核酸分子、蛋白质或病毒的添加、取代或缺失一部分而改变的核酸、蛋白质和病毒。
复制缺陷：在非肿瘤细胞(与肿瘤细胞相比)中表现出“复制缺陷”的病毒是指与肿瘤细胞相比，在正常细胞中表现出病毒复制降低的腺病毒。复制缺陷通过以下证明，例如，与肿瘤细胞相比，在正常细胞中病毒晚期蛋白表达的缺乏、病毒DNA合成的降低、诱导E2F靶基因(例如，细胞周期蛋白A和B)的能力降低、引起S期进入的能力降低和/或诱导细胞杀死的能力降低。
复制缺陷病毒：在具有给定表型的预定细胞群中(例如，具有失调的E2F途径的肿瘤细胞)，优先抑制细胞增殖、引起细胞裂解或诱导细胞凋亡(统称为杀死)的病毒。在不具有所述预定细胞表型的细胞(例如正常的，非肿瘤细胞)中，此类病毒不能减少或抑制细胞增殖、引起细胞裂解、诱导细胞凋亡或者不能复制，或在上述能力方面受限。
自切割肽：诱导核糖体跳过C末端的肽键合成的肽，导致所述肽序列和下游多肽之间的分离。病毒编码的2A肽是一种自切割肽。病毒编码的2A肽包括，例如，来自猪捷申病毒-1(PTV1)、口蹄疫病毒(FMDV)、马鼻炎A病毒(ERAV)和明脉扁刺蛾β四体病毒(TaV)的2A肽。
序列一致性：两个或更多个核酸序列、或两个或更多个氨基酸序列之间的一致性或相似性，依据所述序列之间的一致性或相似性表示。序列一致性可依据百分比一致性来测量；百分比越高，序列就越一致。序列相似性可依据百分比相似性(其考虑了保守氨基酸取代)来测量；百分比越高，序列就越相似。当使用标准方法比对时，核酸或氨基酸序列的同源物或直系同源物具有相对高程度的序列同一性/相似性。与关系更远的(more distantlyrelated)物种(例如人和C.线虫(C.elegans)序列)相比，当直系同源蛋白质或cDNA来源于更密切相关的物种(例如人和小鼠序列)时，这种同源性更为显著。
用于比较的序列比对方法是本领域公知的。各种程序和比对算法描述在：Smith&Waterman,Adv.Appl.Math.2:482,1981；Needleman&Wunsch,J.Mol.Biol.48:443,1970；Pearson&Lipman,Proc.Natl.Acad.Sci.USA 85:2444,1988；Higgins&Sharp,Gene,73:237-44,1988；Higgins&Sharp,CABIOS 5:151-3,1989；Corpet等,Nuc.Acids Res.16:10881-90,1988；Huang等Computer Appls.in the Biosciences 8,155-65,1992；以及Pearson等,Meth.Mol.Bio.24:307-31,1994.Altschul等,J.Mol.Biol.215:403-10,1990中，显示了序列比对方法和同源性计算的详细考虑。
NCBI基本局部比对搜索工具(BLAST)(Altschul等，J.Mol.Biol.215:403-10,1990)可从几个来源获得，包括国家生物信息中心(National Center for BiologicalInformation(NCBI))和互联网，用于与序列分析程序blastp、blastn、blastx、tblastn和tblastx相连。额外的信息可以在NCBI网站上找到。
血清型：由一组特有的抗原相区分的一组密切相关的微生物(如病毒)。
受试者：活的多细胞脊椎动物生物体，包括人和非人哺乳动物的一个类别。
合成：在实验室中通过人工手段产生，例如合成核酸或蛋白质可在实验室中化学合成。
治疗剂：当向受试者适当地施用时能够诱导所需治疗性或预防效果的任何制剂。治疗剂包括但不限于化学化合物、小分子、重组病毒、反义化合物、抗体(或其抗原结合片段)、肽或核酸分子。例如，用于治疗癌症的治疗剂包括预防或抑制肿瘤生长、肿瘤进展或肿瘤转移的制剂。“治疗性蛋白”是治疗剂，其是蛋白质或肽，包括抗体或其抗原结合片段。在本文的一些实施方案中，所述治疗性蛋白是免疫调节剂。在其他实施方案中，所述治疗性蛋白包括毒素、Fas或FasL、可溶性死亡因子、旁立破损介导物(mediator of bystanderdestruction)、肿瘤抗原、neo抗原或同种异体抗原。
治疗有效量：特定药学试剂或治疗剂(例如重组病毒)的量，其足以在用该试剂治疗的受试者或细胞中达到所需效果。所述试剂的有效量可取决于若干因素，包括但不限于所治疗的受试者或细胞，以及治疗性组合物的施用方式。
Uexon：位于早期E3区和纤维基因之间的l链(向左转录)上的开放阅读框(Tollefson等,J Virol 81(23):12918-12926)。
除非另有说明，本文所使用的所有技术和科学术语具有与本公开所属的领域的普通技术人员所通常理解的一样的含义。单数形式的“a”、“an”和“the”包括复数指代物，除非上下文另有明确说明。“comprising A or B(包括A或B)”意指“including A,B,or A andB”(包括A、B、或A和B)。还将理解的是，针对核酸或多肽所给出的所有碱基大小或氨基酸大小，以及所有分子量或分子质量值是近似的，并且被提供用以描述。尽管与本文中所描述的那些相类似或者相等同的方法和材料可用于实施或测试本公开，但下文则描述了适合的方法和材料。通过引用将本文提及的所有出版物、专利申请、专利以及其他参考文献以其全文并入。如有矛盾，以包括术语解释的本说明书为准。此外，所述材料、方法以及实施例仅是阐释性的，而无意进行限制。
III.实施方案概述
本文公开的是包括异源开放阅读框(ORF)和自切割肽编码序列的重组腺病毒基因组。所述异源ORF可编码，例如，治疗性蛋白(诸如免疫调节剂)。由所述公开的基因组产生的所述重组腺病毒基因组和重组腺病毒可用于，例如各种不同的治疗性应用，诸如癌症的治疗。
本文提供重组腺病毒基因组，其包括异源ORF和自切割肽编码序列，两者均可操作地连接至与内源腺病毒ORF相同的阅读框中且在所述相同的阅读框中。所述自切割肽编码序列位于所述异源ORF和所述内源ORF之间。在一些实施方案中，所述内源ORF是E1B-55k，且所述异源ORF在E1B-55k的3'；所述内源ORF是DNA聚合酶，所述异源ORF在DNA聚合酶的5'；所述内源ORF是DNA结合蛋白(DBP)，所述异源ORF在DBP的3'；所述内源ORF是腺病毒死亡蛋白(ADP)，所述异源ORF在ADP的5'；所述内源ORF是E3-14.7k，所述异源ORF在E3-14.7k的3'；所述内源ORF是E4-ORF2，所述异源ORF在E4-ORF2的5'；或者所述内源ORF是纤维蛋白，所述异源ORF在纤维蛋白的3'。
在一些实施方案中，所述异源ORF编码治疗性蛋白。
在一些实施方案中，所述自切割肽是2A肽或其变体。在一些实例中，所述2A肽包括猪捷申病毒-1(PTV1)2A(P2A)肽、口蹄疫病毒(FMDV)2A(F2A)肽、马鼻炎A病毒(ERAV)2A(E2A)肽或明脉扁刺蛾β四体病毒(TaV)2A(T2A)肽，或其变体。在一些具体的实例中，所述2A肽序列与SEQ ID NO:14或SEQ ID NO:18的氨基酸序列至少80％、至少85％、至少90％、至少95％、至少96％、至少97％、至少98％或至少99％一致。在一些实例中，所述2A肽变体包括在N末端的额外的氨基酸序列(诸如GSG)。
在具体的实例中，所述F2A肽序列与SEQ ID NO:15或SEQ ID NO:19的氨基酸序列至少80％、至少85％、至少90％、至少95％、至少96％、至少97％、至少98％或至少99％一致。在具体的实例中，所述E2A肽序列与SEQ ID NO:16或SEQ ID NO:20的氨基酸序列至少80％、至少85％、至少90％、至少95％、至少96％、至少97％、至少98％或至少99％一致。在具体的实例中，所述T2A肽序列与SEQ ID NO:17或SEQ ID NO:21的氨基酸序列至少80％、至少85％、至少90％、至少95％、至少96％、至少97％、至少98％或至少99％一致。在特定的非限制性实例中，所述自切割肽包括SEQ ID NO:14-21中的任何一个的氨基酸序列或由其组成。
在一些实施方案中，所述腺病毒是腺病毒型5(Ad5)。在其他实施方案中，所述腺病毒是Ad2、Ad3、Ad9、Ad11、Ad12或Ad34。还在其他实施方案中，所述腺病毒是嵌合腺病毒，诸如，但不限于，Ad5/Ad9或Ad5/Ad34嵌合腺病毒。
本文进一步提供重组腺病毒，其包括本文公开的重组腺病毒基因组。
本文提供的重组腺病毒(和重组腺病毒基因组)任选地包括额外的修饰，例如将病毒靶向具体细胞类型，以抑制靶向肝脏以及在肝脏中的复制，以允许在肿瘤细胞中选择性复制，和避免预先存在的针对常见腺病毒血清型的中和抗体。额外的修饰可以根据所述重组腺病毒的需要的用途而变化。腺病毒修饰描述于，例如，PCT申请号PCT/US2015/051745(2015年9月23日提交)、WO2012/024350、WO2013/138505和WO2014/153204中，通过引用将其全文并入本文。
本公开还提供包括重组腺病毒基因组或重组腺病毒，和药学上可接受的载体的组合物。
本文还提供将治疗性蛋白递送至受试者的方法。在一些实施方案中，所述方法包括向所述受试者施用本文公开的重组腺病毒基因组、重组腺病毒，或组合物。在这些方法中，所述重组腺病毒基因组或重组腺病毒的异源ORF编码所述治疗性蛋白。
进一步提供减少或抑制肿瘤细胞活力和/或肿瘤细胞生长的方法(与没有所述公开的疗法相比，例如减少至少10％、至少20％、至少30％、至少40％、至少50％、至少75％、至少90％、至少95％或至少98％)。在一些实施方案中，所述方法包括将所述肿瘤细胞与本文公开的重组腺病毒基因组、重组腺病毒或组合物接触。在这些方法中，所述异源ORF编码治疗性蛋白。在一些实例中，所述方法是体外方法。在其他实例中，所述方法是体内方法且接触所述肿瘤细胞包括向患有肿瘤的受试者施用所述重组腺病毒基因组、重组腺病毒或组合物。
本文进一步提供减少或抑制肿瘤进展的方法，诸如通过减少转移(metastasis)的数量和尺寸，或减少受试者内肿瘤的体积(例如与没有所述公开的疗法相比，减少至少10％、至少20％、至少30％、至少40％、至少50％、至少75％、至少90％、至少95％或至少98％)。在一些实施方案中，所述方法包括向所述受试者施用治疗有效量的本文公开的重组腺病毒基因组、重组腺病毒或组合物。在这些方法中，所述异源ORF编码治疗性蛋白。
进一步提供治疗受试者中癌症的方法。在一些实施方案中，所述方法包括向所述受试者施用治疗有效量的本文公开的重组腺病毒基因组、重组腺病毒或组合物。在这些方法中，所述异源ORF编码治疗性蛋白。
在本文的一些实施方案中，所述方法进一步包括向所述受试者施用额外的治疗剂。例如所述额外的治疗剂可包括抗癌剂，诸如化疗剂(a chemotherapeutic agent)、生物剂(a biologic)(诸如抗体或其片段，诸如单克隆抗体)，或核酸分子，诸如抑制性核酸分子)或其他治疗性处理(therapeutic treatment)，例如肿瘤的手术切除或肿瘤的照射。
本文还提供试剂盒，其包括本文公开的重组腺病毒基因组、重组腺病毒或组合物；以及一种或更多种额外的治疗剂和/或一种或更多种诊断剂。在一些实施方案中，所述一种或更多种额外的治疗剂包括化疗剂、生物剂或其组合。在一些实例中，所述一种或更多种诊断剂包括一种或更多种对肿瘤标志物特异性的抗体或核酸分子，或可用于体外或体内追踪所述病毒或肿瘤细胞的成像探针。
IV.外源ORF的最佳放置
所述36kb腺病毒基因组是紧凑的，使用顶部和底部链来编码各种基因。在所述腺病毒基因组内的许多位置，顶部和底部链都同时用于编码分开的基因。所述基因组大小已进化为对于插入其衣壳是最佳的。结果，外源基因的插入受到所述衣壳大小容量的限制，因为外源核酸的过量添加导致不完整的基因组加载到衣壳中并降低病毒动力学。
针对由腺病毒基因组中有限的可用空间呈现的挑战的解决方案是，将外源开放阅读框(ORF)定位在天然腺病毒ORF内作为融合产物。该策略利用已经在基因组中编码的腺病毒启动子、5'UTR和polyA尾巴。然而，天然腺病毒蛋白和外源蛋白之间融合的表达可能对一种或两种蛋白质功能有害，并导致腺病毒复制动力学的显著降低。
本公开通过使用置于所述天然(内源)ORF和所述外源(异源)ORF之间的自切割肽序列提供了对该问题的解决方案。当置于单个mRNA上的所述两个ORF之间时，自切割肽序列的存在导致核糖体跳过并释放与第二蛋白分开的第一蛋白。在本文公开的一些实施方案中，所述自切割肽是2A肽(P2A)。
本文还公开了腺病毒基因组内异源ORF的最佳放置位点的鉴定。自切割肽序列和异源ORF明智放置的组合导致高表达以及对病毒动力学的最小影响。
如下文实施例1中所述，鉴定了所述腺病毒基因组内的几个位点，在插入异源ORF后，不抑制腺病毒的复制动力学。特别地，确定了可将异源ORF插入到所述E1B-55k ORF的C末端，所述DNA聚合酶ORF的N末端，所述DBP ORF的C末端，所述ADP ORF的N末端，所述E3-14.7k ORF的C末端或所述E4-ORF2的N末端。在每种情况下，在所述腺病毒ORF和所述异源ORF之间插入自切割肽序列(P2A位点)。本文进一步公开了将异源ORF插入到纤维蛋白的C-末端以产生复制缺陷型腺病毒；然而，所述重组病毒能够在感染细胞中产生异常高水平的异源蛋白，其可证明在许多治疗性应用中是有用的。
因此，本公开考虑以下重组腺病毒用于治疗性应用的用途(其中“SC”是指编码自切割肽的序列，诸如P2A)：
E1B-55k-SC-异源ORF
异源ORF-SC-(DNA聚合酶)
DBP-SC-异源ORF
异源ORF-SC-ADP
E3-14.7k-SC-异源ORF
异源ORF-SC-E4-ORF2
纤维蛋白-SC-异源ORF
在本文的一些实施方案中，所述自切割肽是病毒编码的2A肽，或如下文进一步描述的其经修饰的版本。
V.自切割肽序列
自切割肽序列是诱导核糖体跳过C末端肽键合成的肽，导致所述肽序列末端与下游多肽之间的分离。自切割肽的使用允许从单个ORF表达在所述自切割肽侧翼的多种蛋白。病毒编码的2A肽是一种自切割肽。
与其他自切割肽一样，2A肽通过使核糖体跳过2A元件C末端肽键合成而起作用，导致所述2A序列末端与下游肽之间的分离(Kim等,PLoS One 6(4):e18556,2011)。所述“切割”发生在所述2A肽的C末端上发现的甘氨酸和脯氨酸残基之间。示例性2A肽包括但不限于，由明脉扁刺蛾β四体病毒(TaV)、马鼻炎A病毒(ERAV)、猪捷申病毒-1(PTV1)和口蹄疫病毒(FMDV)编码的2A肽或其修饰版本。
在本文具体的实例中，所述2A肽包括PTV1 2A(P2A)、FMDV 2A(F2A)、ERAV 2A(E2A)或TaV 2A(T2A)，其序列如下所示，并在本文中列为SEQ ID NO:14-17。
P2A:ATNFSLLKQAGDVEENPGP(SEQ ID NO:14)
F2A:VKQTLNFDLLKLAGDVESNPGP(SEQ ID NO:15)
E2A:QCTNYALLKLAGDVESNPGP(SEQ ID NO:16)
T2A:EGRGSLLTCGDVEENPGP(SEQ ID NO:17)
在一些实例中，所述2A肽被修饰为在N末端包括Gly-Ser-Gly以提高切割效率。经修饰的P2A、F2A、E2A和T2A的序列如下所示，并在本文中列为SEQ ID NO:18-21。
经修饰的P2A：GSGATNFSLLKQAGDVEENPGP(SEQ ID NO:18)
经修饰的F2A：GSGVKQTLNFDLLKLAGDVESNPGP(SEQ ID NO:19)
经修饰的E2A：GSGQCTNYALLKLAGDVESNPGP(SEQ ID NO:20)
经修饰的T2A：GSGEGRGSLLTCGDVEENPGP(SEQ ID NO:21)
在一些实施方案中，所述2A多肽是本文公开的2A多肽的变体。变体可包括与野生型或本文公开的经修饰的2A多肽具有至少80％、至少85％、至少90％、至少95％、至少96％、至少97％、至少98％或至少99％或更多序列一致性的多肽序列。变体可包括，例如，SEQ IDNO:14-21中的任何一个2A多肽的N末端至少一个氨基酸的缺失，例如1、2、3、4或5个氨基酸的缺失，包括在任何两个所列值之间的范围。变体可包括，例如，SEQ ID NO:14-21中的任何一个2A多肽的C末端至少一个氨基酸的缺失，例如1、2、3、4或5个氨基酸的缺失，包括在任何两个所列值之间的范围。变体还可包括，例如，至少1、2、3、4或5个氨基酸的取代，诸如保守氨基酸取代。
VI.药物组合物
本文提供了包括重组腺病毒或重组腺病毒基因组的组合物。所述组合物，任选地，适于体外或体内剂型(formulation)和施用。任选地，所述组合物包括所提供的制剂的一种或更多种和药学上可接受的载体。合适的载体及其剂型描述于Remington:The Scienceand Practice of Pharmacy,22nd Edition,Loyd V.Allen等编辑。药学上可接受的载体包括非生物学上或其他方面不合需要的材料，即，将所述材料施用于受试者而不会引起不需要的生物学效应或以有害的方式与包括它的药物组合物的其他组分相互作用。如果向受试者施用者，所述载体被任选地选择以使所述活性成分的降解最小化并使所述受试者中的不良副作用最小化。
将所述重组病毒(或一种或更多种编码所述重组腺病毒的核酸或载体)根据已知的方法施用，诸如静脉施用，例如，作为丸剂或通过一段时间连续灌注，通过肌肉内、腹膜内、脑脊髓内(intracerobrospinal)、皮下、关节内、滑膜内、鞘内，口服，局部、肿瘤内或吸入途径。所述施用可以是局部的或全身的。所述组合物可以通过几种施用途径中的任何一种施用，包括局部、口服、肠胃外、静脉内、关节内、腹膜内、肌肉内、皮下、腔内、透皮，肝内(intrahepatically)、颅内、雾化/吸入，或通过支气管镜安装。因此，所述组合物以多种方式施用，所述方式取决于是否需要局部或全身治疗，以及待治疗的区域。
在一些实施方案中，用于施用的所述组合物将包括溶入药学上可接受的载体(优选含水载体)的本文描述的重组腺病毒(或重组基因组)。可以使用各种含水载体，例如缓冲盐水等。这些溶液是无菌的并且通常没有不需要的物质。这些组合物可通过常规的公知的灭菌技术灭菌。所述组合物可含有接近生理条件所需的药学上可接受的辅助物质，例如pH调节剂和缓冲剂、毒性调节剂制剂等，例如乙酸钠、氯化钠、氯化钾、氯化钙、乳酸钠等。这些剂型中活性剂的浓度可以广泛变化，并且将根据所选择的具体施用模式和受试者的需要主要基于流体体积、粘度、体重等来选择。
药物剂型，特别是重组病毒的药物剂型可通过将具有所需纯度的重组腺病毒(或编码重组腺病毒的一种或更多种核酸)与任选的药学上可接受的载体、赋形剂或稳定剂混合来制备。此类制剂可以是冻干剂型或水溶液。
可接受的载体、赋形剂或稳定剂在所使用的剂量和浓度下对接受者是无毒的。可接受的载体、赋形剂或稳定剂可以是乙酸盐、磷酸盐、柠檬酸盐和其他有机酸；抗氧化剂(如抗坏血酸)防腐剂、低分子量多肽；蛋白质如血清白蛋白或明胶，或亲水聚合物如聚乙烯吡咯烷酮(polyvinylpyllolidone)；和氨基酸、单糖、二糖和包括葡萄糖、甘露糖或糊精等的其他碳水化合物；螯合剂；和离子和非离子表面活性剂(例如聚山梨醇酯)；成盐抗衡离子如钠；金属络合物(例如锌-蛋白质络合物)；和/或非离子表面活性剂。所述重组腺病毒(或编码重组腺病毒的一种或更多种核酸)可以以任何合适浓度的感染单位配制。
适于口服施用的制剂可以由以下组成(a)液体溶液，诸如悬浮在稀释剂，诸如水、盐水或PEG 400中的有效量的所述重组腺病毒；(b)胶囊、小袋(sachets)或片剂，每种含有预定量的活性成分，如液体、固体、颗粒或明胶；(c)适当液体中的悬浮液；以及(d)合适的乳液。片剂形式可包括乳糖、蔗糖、甘露醇、山梨糖醇、磷酸钙、玉米淀粉、马铃薯淀粉、微晶纤维素、明胶、胶体二氧化硅、滑石、硬脂酸镁、硬脂酸，和其他赋形剂、着色剂、填充剂、粘合剂、稀释剂、缓冲剂、润湿剂、防腐剂、调味剂、染料、崩解剂，和药学上相容的载体中的一种或更多种。锭剂(lozenge)形式可将活性成分包含在调味剂中，例如蔗糖，以及软锭剂(pastille)将活性成分包含在惰性基质中，例如除了活性成分之外，还包含本领域已知的载体的明胶和甘油或蔗糖和阿拉伯胶乳剂、凝胶等。
所述重组腺病毒(或编码重组腺病毒的一种或更多种核酸)，单独或与其他合适的组分组合，可被制成气溶胶剂型(即，它们可以被“雾化”)以通过吸入施用。可以将气溶胶剂型置于加压的可接受的推进剂中，例如二氯二氟甲烷、丙烷、氮气等。
适合于肠胃外施用的剂型，诸如，例如，通过关节内(在关节中)、静脉内、肌肉内、肿瘤内、真皮内、腹膜内和皮下途径，包括水性和非水性、等渗无菌注射溶液，其可含有使剂型与预期(intended)接受者的血液等渗的抗氧化剂、缓冲剂、抑菌剂和溶质，以及可包括悬浮剂、增溶剂、增稠剂、稳定剂和防腐剂的水性和非水性无菌悬浮液。在所提供的方法中，组合物可以例如通过静脉内输注、口服、局部、腹膜内、膀胱内肿瘤内或鞘内施用。肠胃外施用、肿瘤内施用和静脉内施用是优选的施用方法。化合物的所述剂型可以在单位剂量或多剂量密封容器中呈现，例如安瓿和小瓶。
注射溶液和悬浮液可由前述类型的无菌粉末、颗粒和片剂制备。用于离体治疗的由腺病毒转导或感染的细胞或用核酸转染的细胞也可以如上所述的静脉内或胃肠外施用。
在一些实例中，所述药物制剂(preparation)是单位剂型(dosage form)。在这种形式中，将所述制剂细分为含有适量所述活性成分的单位剂量(doses)。因此，根据所述施用方法，所述药物组合物可以多种单位剂量形式施用。例如，适于口服施用的单位剂型包括但不限于粉末、片剂、丸剂、胶囊和锭剂。
VII.治疗方法
可施用本文公开的重组腺病毒、重组腺病毒基因组和组合物用于治疗性或预防性治疗。特别地，提供减少或抑制受试者内肿瘤活力或生长，减少或抑制受试者内肿瘤进展，减少受试者内转移的数量，减少受试者内转移的大小和/或体积，减少受试者内肿瘤大小和/或体积和/或治疗受试者中癌症的方法。所述方法包括向所述受试者施用治疗有效量的重组腺病毒基因组或重组腺病毒(或其组合物)。如全文所描述的，所述腺病毒或药物组合物以任何数量的方式施用，所述方式包括但不限于静脉内、血管内、鞘内、肌肉内、皮下、肿瘤内、腹膜内或口服。任选地，所述方法进一步包括向所述受试者施用一种或更多种额外的治疗剂，诸如化疗剂、生物剂和/或辐射。
在一些实施方案中，所述癌症或肿瘤是肺、前列腺、结肠直肠、乳腺、甲状腺、肾、胰腺、骨、头颈或肝的癌症或肿瘤，或者是一种类型的白血病。在一些情况下，所述癌症是转移性的。在一些实例中，所述肿瘤是乳腺、垂体、甲状腺或前列腺的肿瘤；脑、肝、脑膜、骨、卵巢、子宫或子宫颈的肿瘤；单核细胞或髓性白血病；腺癌、腺瘤、星形细胞瘤、膀胱肿瘤、脑肿瘤、伯基特淋巴瘤(Burkitt’s lymphoma)、乳腺癌、宫颈癌、结肠癌(colon carcinoma)、肾癌、肝癌、肺癌、卵巢癌、胰腺癌、前列腺癌、直肠癌、皮肤癌、胃癌、睾丸癌、甲状腺癌、软骨肉瘤、绒毛膜癌、纤维瘤、纤维肉瘤、成胶质细胞瘤(glioblastoma)、胶质瘤、肝癌、组织细胞瘤、成平滑肌瘤(leiomyoblastoma)、平滑肌肉瘤、淋巴瘤、脂肪肉瘤细胞、乳腺肿瘤、成神经管细胞瘤、骨髓瘤、浆细胞瘤、成神经细胞瘤、神经胶质瘤、成骨肉瘤、胰腺肿瘤、垂体肿瘤、成视网膜细胞瘤、横纹肌肉瘤、肉瘤、睾丸肿瘤、胸腺瘤或肾母细胞瘤(Wilms tumor)。肿瘤包括原发性和转移性实体瘤，包括乳腺、结肠、直肠、肺、口咽、下咽、食道、胃、胰腺、肝、胆囊和胆管、小肠、尿路(包括肾、膀胱和尿路上皮)、女性生殖道(包括子宫颈、子宫和卵巢以及绒毛膜癌和妊娠滋养细胞疾病)、男性生殖道(包括前列腺、精囊、睾丸和生殖细胞肿瘤)、内分泌腺(包括甲状腺、肾上腺和垂体腺)，和皮肤的癌，以及血管瘤、黑色素瘤、肉瘤(包括由骨和软组织引起的肉瘤以及卡波济氏肉瘤(Kaposi's sarcoma))和脑、神经、眼睛和脑膜的肿瘤(包括星形细胞瘤、胶质瘤、成胶质细胞瘤、成视网膜细胞瘤、神经瘤、成神经细胞瘤、神经鞘瘤(Schwannomas)和脑膜瘤)。在某些方面，可以治疗由造血系统恶性肿瘤例如白血病引起的实体瘤(即绿色瘤(chloromas)，浆细胞瘤和蕈样真菌病和皮肤T细胞淋巴瘤/白血病的斑块和肿瘤)以及淋巴瘤(霍奇金和非霍奇金氏淋巴瘤)的治疗中。此外，治疗可用于预防本文所描述的肿瘤的转移。
在治疗性应用中，将重组腺病毒或其组合物以治疗有效量或剂量向受试者施用。针对此用途有效的量取决于所述疾病的严重程度和患者健康的一般状态。可以根据患者所需的和耐受的剂量和频率施用组合物的单次或多次施用。“患者”或“受试者”包括人和其他动物，特别是哺乳动物。因此，所述方法适用于人类治疗和兽医应用，诸如小鼠、大鼠、兔、猫、狗、牛、马、猪、鸡等。
具有修饰序列的腺病毒的有效量是基于个体确定的，并且至少部分基于所使用的具体的重组腺病毒；个人的大小、年龄、性别；以及所述增殖细胞的大小和其他特征。例如，对于人的治疗，使用至少103个噬斑形成单位(plaque forming unit(PFU))的重组病毒，例如至少104、至少105、至少106、至少107、至少108、至少109、至少1010、至少1011或至少1012PFU，例如使用约103至1012PFU的重组病毒，取决于存在的增殖细胞或肿瘤的类型、大小和数量。所述有效量可以是从约1.0pfu/kg体重至约1015pfu/kg体重(例如，从约102pfu/kg体重至约1013pfu/kg体重)。重组腺病毒以单剂量或多剂量(例如，二、三、四、六或更多剂量)施用。多剂量可以同时或连续使用(例如，数天或数周)。
在一些实施方案中，所提供的方法包括向所述受试者施用一种或更多种额外的治疗剂，诸如抗癌剂或其他治疗性治疗(诸如所述肿瘤的手术切除)。示例性的抗癌剂包括，但不限于化疗剂，诸如，例如，有丝分裂抑制剂、烷化剂、抗代谢物、插入抗生素(intercalating antibiotics)、生长因子抑制剂、细胞周期抑制剂、酶、拓扑异构酶抑制剂、抗生存剂、生物反应调节剂、抗激素(例如抗雄激素)、抗血管生成剂和CDK抑制剂。其他抗癌治疗包括放射治疗以及特异性靶向癌细胞的其他抗体。
烷基化剂的非限制性实例包括氮芥(例如二氯甲基二乙胺(mechlorethamine)、环磷酰胺、美法仑、尿嘧啶氮芥或苯丁酸氮芥(chlorambucil))、烷基磺酸盐(例如白消安)、亚硝基脲(例如卡莫司汀、洛莫司汀(lomustine)、司莫司汀(semustine)、链脲霉素或达卡巴嗪)。
抗代谢物的非限制性实例包括叶酸类似物(例如甲氨蝶呤)嘧啶类似物(例如5-FU或阿糖胞苷)和嘌呤类似物，例如巯嘌呤或硫鸟嘌呤。
天然产物的非限制性实例包括长春花生物碱(例如长春碱、长春新碱或长春地辛)、表鬼臼毒素(例如依托泊苷或替尼泊苷)、抗生素(例如放线菌素、柔红霉素、多柔比星(doxorubicin)、博来霉素、普卡霉素或丝裂霉素C)和酶(如L-天冬酰胺酶)。
混杂制剂的非限制性实例包括铂配合物(如顺-二胺-二氯铂II，也称为顺铂)、取代脲(如羟基脲)、甲基肼衍生物(如丙卡巴肼)和肾上腺抑制剂(如米托坦和氨鲁米特)。
激素和拮抗剂的非限制性实例包括肾上腺皮质激素(例如泼尼松)、孕激素(例如己酸羟孕酮、醋酸甲羟孕酮和醋酸甲地孕酮(magestrol acetate))、雌激素(例如己烯雌酚和乙炔雌二醇)、抗雌激素(例如三苯氧胺)和雄激素(例如丙酸睾丸酮(testeroneproprionate)和氟甲睾酮)。
最常用的化疗药物的实例包括阿霉素、美法仑(Alkeran)、阿糖胞苷(Ara-C)、BiCNU、白消安、CCNU，卡铂(Carboplatinum)、顺铂、环磷酰胺(Cytoxan)、道诺霉素、DTIC、5-FU、氟达拉滨、羟基脲(Hydrea)、伊达比星(Idarubicin)、异环磷酰胺、氨甲喋呤、光神霉素、丝裂霉素、米托蒽醌、氮芥、紫杉醇(或其他紫杉烷、如多西紫杉醇)、长春花碱(Velban)、长春新碱、VP-16、而一些更新的药物包括吉西他滨(健择(Gemzar))、赫赛汀、伊立替康(Irinotecan)(伊立替康(Camptosar)、CPT-11)、克拉屈滨(Leustatin)、诺维苯(Navelbine)、利妥昔单抗STI-571、泰索帝、拓扑替康(美新(Hycamtin))、希罗达(卡培他滨)、泽文林(Zevelin)和骨化三醇。
可以使用的免疫调节剂的非限制性实例包括AS-101(Wyeth-Ayerst Labs.)、溴匹立明(bropirimine)(Upjohn)、γ干扰素(Genentech)、GM-CSF(粒细胞巨噬细胞集落刺激因子；遗传学研究所)、IL-2(Cetus或Hoffman-LaRoche)、人免疫球蛋白(CutterBiological)，IMREG(来自Imreg of New Orleans，La.)、SK&F 106528和TNF(肿瘤坏死因子；Genentech)。
CDK(细胞周期蛋白依赖性激酶)抑制剂是抑制CDK功能的制剂。用于所提供的方法的CDK抑制剂的非限制性实例包括AG-024322、AT7519、AZD5438、夫拉平度(flavopiridol)、尹地苏拉姆(indisulam)、P1446A-05、PD-0332991和P276-00(参见例如Lapenna等、NatureReviews,8:547-566,2009)。其他CDK抑制剂包括LY2835219、帕布昔利布(Palbociclib)、LEE011(Novartis)、pan-CDK抑制剂AT7519、西里昔利布(seliciclib)、CYC065、丁内酯I、黑美妮奥地辛(hymenialdisine)、SU9516、CINK4、PD0183812或海洋活性生物碱(fascaplysin)。
在一些实例中，CDK抑制剂是广谱抑制剂(例如夫拉平度(flavopiridol)、奥罗莫星(olomoucine)、柔丝扣维亭(roscovitine)、肯普龙(kenpaullone)、SNS-032、AT7519、AG-024322、(S)-柔丝扣维亭或R547)。在其他实例中，CDK抑制剂是特异性抑制剂(例如、海洋活性生物碱(fascaplysin)、柔维亭(ryuvidine)、泊伐拉诺A(purvalanol A)、NU2058、BML-259、SU 9516、PD0332991或P-276-00)。
在一些实施方案中，所提供的方法进一步包括向受试者施用治疗有效量的免疫疗法。可以使用的免疫调节剂的非限制性实例包括AS-101(Wyeth-Ayerst Labs.)、溴匹立明(bropirimine)(Upjohn)、γ干扰素(Genentech)、GM-CSF(粒细胞巨噬细胞集落刺激因子；遗传学研究所)、IL-2(Cetus或Hoffman-LaRoche)、人免疫球蛋白(Cutter Biological)、IMREG(来自Imreg of New Orleans,La.)、SK&F 106528和TNF(肿瘤坏死因子；Genentech)。所述免疫治疗剂制剂可以是PD-1拮抗剂或PD-L1拮抗剂，例如特异性结合PD-1或PD-L1的抗体，例如阿特左丽珠单抗(Atezolizumab)、MPDL3280A、BNS-936558(纳武单抗(Nivolumab))、派姆单抗(Pembrolizumab)、匹地丽珠单抗(Pidilizumab)、CT011、AMP-224、AMP-514、MEDI-0680、BMS-936559、BMS935559、MEDI-4736、MPDL-3280A、MSB-0010718C。所述免疫治疗剂制剂也可以是CTLA-4、LAG-3或B7-H3拮抗剂，例如揣摩丽木单抗(Tremelimumab)、BMS-986016和MGA271。
在一些实施方案中，所提供的方法还包括向受试者施用治疗有效量的一种或更多种抗血管生成剂，例如对减少甚至抑制血管生长起作用的蛋白质、酶、多糖、寡核苷酸、DNA，RNA和重组载体以及小分子。合适的血管生成抑制剂的实例包括，但不限于血管抑制素K1-3，星形孢菌素(staurosporine)、染料木黄酮、烟曲霉素、甲羟孕酮、苏拉明、干扰素-α、金属蛋白酶抑制剂、血小板因子4、生长抑素、血小板反应蛋白(thromobospondin)、内皮抑素、沙利度胺及其衍生物和类似物。例如，在一些实施方案中，所述抗血管生成剂是特异性结合VEGF(例如，Roche)或VEGF受体(例如VEGFR2抗体)的抗体。在一个实例中，所述抗血管生成剂包括VEGFR2抗体或DMXAA(也称为伐地莫赞(Vadimezan)或ASA404；可商购，例如来自Sigma Corp.,St.Louis,MO)或两者。抗血管生成剂制剂可以是贝伐单抗、舒尼替尼、抗血管生成酪氨酸激酶抑制剂(TKI)，例如舒尼替尼、西替尼(xitinib)和达沙替尼。这些可以单独使用或以任何组合使用。
在一些实施方案中，所提供的方法进一步包括向受试者施用治疗有效量的一种或更多种激酶抑制剂，例如和舒尼替尼，索拉非尼，阿尼替尼(anitinib)和达沙替尼，其阻止生长因子的磷酸化和激活。可以使用的抗体包括阻断生长因子和血管生成途径的和这些可以单独使用或组合使用。
在一些实施方案中，所提供的方法还包括向受试者施用治疗有效量的一种或更多种治疗性单克隆抗体，例如3F8、阿巴伏单抗(Abagovomab)、阿得妥莫单抗(Adecatumumab)、阿夫土珠(Afutuzumab)、阿拉西珠(Alacizumab)、阿伦单抗(Alemtuzumab)、阿妥莫单抗三胺五乙酸(Altumomab pentetate)、阿那妥莫单抗马福纳托克斯(Anatumomab mafenatox)、阿泊朱单抗(Apolizumab)、阿西莫单抗(Arcitumomab)、巴维昔单抗(Bavituximab)、贝妥莫单抗(Bectumomab)、贝利木单抗(Belimumab)、贝索单抗(Besilesomab)、贝伐单抗(Bevacizumab)、比伐土木珠单抗莫坦辛(Bivatuzumab mertansine)、博利纳吐单抗(Blinatumomab)、伯仁土西单抗外都亭(Brentuximab vedotin)、坎土珠单抗莫坦辛(Cantuzumab mertansine)、卡罗单抗喷地肽(Capromab pendetide)、卡妥索单抗(Catumaxomab)、CC49、西妥昔单抗(Cetuximab)、西他土珠波咖托克斯(Citatuzumabbogatox)、西妥木单抗(Cixutumumab)、西利伐土珠单抗特出克斯坦(Clivatuzumabtetraxetan)、叩娜土木单抗(Conatumumab)、达西土珠单抗(Dacetuzumab)、地莫单抗(Detumomab)、艾叩莫西单抗(Ecromeximab)、依库丽单抗(Eculizumab)、依决洛单抗(Edrecolomab)、依帕珠单抗(Epratuzumab)、二土麦克斯单抗(Ertumaxomab)、伊瑞西珠(Etaracizumab)、伐勒木珠单抗(Farletuzumab)、菲吉土木珠单抗(Figitumumab)、咖利西单抗(Galiximab)、吉姆单抗奥佐米星(Gemtuzumab ozogamicin)、(Girentuximab)、吉姆单抗外都亭(Glembatumumab vedotin)、替伊莫单抗(Ibritumomab tiuxetan)、伊戈伏单抗(Igovomab)、英西单抗(Imciromab)、因特土木单抗(Intetumumab)、伊诺土珠单抗奥佐米星(Inotuzumab ozogamicin)、伊匹单抗(Ipilimumab)、伊拉土木单抗(Iratumumab)、拉博土珠单抗(Labetuzumab)、来沙木单抗(Lexatumumab)、林妥珠单抗(Lintuzumab)、罗伐土珠单抗莫坦辛(Lorvotuzumab mertansine)、鲁卡土木单抗(Lucatumumab)、昔单抗(Lumiliximab)、(Mapatumumab)、马妥珠单抗(Matuzumab)、美泊利单抗(Mepolizumab)、迈特利木单抗(Metelimumab)、利拉土珠单抗(Milatuzumab)、利土莫单抗(Mitumomab)、莫若利木单抗(Morolimumab)、那可罗单抗他发娜托克斯(Nacolomab tafenatox)、那普土莫单抗伊丝他发娜托克斯(Naptumomab estafenatox)、那西土木单抗(Necitumumab)、诺夫土莫单抗莫盆坦(Nofetumomab merpentan)、欧发土木单抗(Ofatumumab)、欧拉热土单抗(Olaratumab)、欧普热土木单抗莫娜托克斯(Oportuzumab monatox)、奥戈伏单抗(Oregovomab)、帕尼单抗(Panitumumab)、喷土莫单抗(Pemtumomab)、帕妥珠单抗(Pertuzumab)、拼土莫单抗(Pintumomab)、普托木单抗(Pritumumab)、雷莫芦单抗(Ramucirumab)、(Rilotumumab)、利妥昔单抗、罗巴土木单抗(Robatumumab)、沙妥莫单抗喷地肽(Satumomab pendetide)、西博土珠单抗(Sibrotuzumab)、索耐普西珠单抗(Sonepcizumab)、塔卡土珠单抗特出克斯坦(Tacatuzumab tetraxetan)、他普利土莫单抗帕普托克斯(Taplitumomab paptox)、泰纳土莫单抗(Tenatumomab)、TGN1412、提西丽木单抗(揣摩丽木单抗)((Ticilimumab(tremelimumab))、提噶土珠单抗(Tigatuzumab)、TNX-650、曲妥单抗(Trastuzumab)、揣摩丽木单抗(Tremelimumab)、土口土珠单抗西莫白介素(Tucotuzumab celmoleukin)、维尔土珠单抗(Veltuzumab)、维洛西单抗(Volociximab)、伏妥莫单抗(Votumumab)或扎鲁土木单抗(Zalutumumab)。在一些实例中，所述异源ORF编码这些治疗抗体中的一个。
针对一些类型的癌症的另一个常见的治疗是手术治疗，例如所述肿瘤或其部分的手术切除。治疗的另一个实例是放疗，例如向所述肿瘤部位施用放射性物质或能量(如外部束治疗)以帮助根除肿瘤或在手术切除前将其缩小。
制剂和剂量的选择可以由本领域技术人员基于所治疗的给定疾病容易地确定。药剂的组合或组合物可以附随地(concomitantly)(例如，作为混合物)、分离地但同时地(例如，通过分离的静脉内管线)或顺序地施用(例如，首先施用一种制剂，然后施用第二制剂)。因此，所述术语组合用于指两种或更多种制剂或组合物的附随、同时或顺序施用。
根据本文公开的方法，向所述受试者施用有效量的一种或更多种本文提供的制剂。将所述有效量定义为产生所需的生理学反应(例如，癌细胞杀死)所必须的任何量。治疗剂通常以每日约0.001mg/kg至约1000mg/kg的初始剂量施用。可使用约0.01mg/kg至约500mg/kg、或约0.1mg/kg至约200mg/kg、或约1mg/kg至约100mg/kg、或约10mg/kg至约50mg/kg的剂量(dose)范围。然而，所述计量，剂量可以根据所述受试者的要求、所治疗病况的严重程度和所用化合物而变化。例如，考虑在具体受试者中诊断的癌症的类型和阶段，可以凭经验确定剂量。在所提供的方法的背景下，向受试者施用的剂量应足以影响患者随时间的有益治疗反应。确定具体情况的适当剂量在从业者的技能范围内。因此，本领域技术人员可凭经验确定用于施用所述制剂的有效量和时间表。所述剂量不应大到引起实质的不良副作用，例如不需要的交叉反应、过敏反应等。如果发生任何禁忌症，个体医师可调整剂量。可以在所述文献中找到针对给定类别的药物产品的适当剂量的指导。
本文提供通过将肿瘤细胞与如本文公开的重组腺病毒、重组腺病毒基因或其组合物接触抑制所述肿瘤细胞活力或生长的方法。在一些实施方案中，所述方法是体外方法。在其他实施方案中，所述方法是体内的方法且接触所述肿瘤病毒包括向患有肿瘤的受试者施用所述重组腺病毒基因组、重组腺病毒或组合物。
进一步提供抑制肿瘤进展或减少受试者内肿瘤体积的方法，通过向所述受试者施用本文公开的治疗有效量的重组腺病毒基因组或重组腺病毒(或其组合物)。
还提供在受试者中治疗癌症的方法，通过向所述受试者施用本文公开的治疗有效量的重组腺病毒基因组或重组腺病毒(或其组合物)。
VIII.Adsembly和AdSLIC
腺病毒基因组被分成几个功能组，标记为E1、E2、E3、E4和L1-5。所述E1区编码控制所有其他病毒基因的转录并在所述宿主细胞中诱导S期的蛋白质。所述E2区编码驱动病毒DNA复制的蛋白质。所述E3区蛋白质调节宿主细胞免疫应答并且在细胞培养中是非必要的。所述E4区含有用于不同功能集的基因。以及所述L1-5区编码病毒粒子结构蛋白。
利用这种功能的自然隔离，发明人先前开发了一种重组腺病毒组装方法，其允许通过将36kb Ad基因组分成4个质粒E1、E3、E4和核心来快速且容易地操作所述36kb Ad基因组，如图6A所示(Adsembly和AdSLIC；参见WO2012/024351，其通过引用并入本文)。由于它们更合理的大小，使用标准技术操作这些较小的质粒是直截了当的。
Adsembly和AdSLIC使之能在4小时内从兼容的基因组文库部分组合性地体外组装具有新性质的腺病毒。Adsembly和AdSLIC提供了一种通用的基因组设计平台，其使之能使用四个库的功能部分组装具有新性质的合成病毒(图6A)。这些部分的库(These librariesof parts)可以使用多位点特异性重组位点(Adsembly；图6B)或非序列依赖性无缝克隆(AdSLIC；图6C)以所有可能的组合重新组装。
所述Adsembly和AdSLIC技术使分子设计和产生具有独特性能的腺病毒成为可能。开发以自动化、高通量方式设计、制造和测试病毒的性能将加速和扩展用于治疗、诊断和可研性学习的新病毒的发展。
虽然克隆步骤曾经是生产新病毒构建的瓶颈，但Adsembly和AdSLIC的出现使得构建病毒基因组的能力已经超过了测试它们的能力。同样高通量的动力学测定对于使用所述Adsembly和AdSLIC方法开发合成的和个性化病毒治疗和诊断的全部潜力和高含量组装是至关重要的。
提供以下实施例以说明某些具体特征和/或实施方案。不应将这些实施例解释为将本公开限制到所描述的具体特征或实施方案。
实施例
实施例1：外源ORF在腺病毒基因组中的最佳位置的鉴定
该实施例描述了腺病毒基因组内特定位置的鉴定，于此处可插入外源ORF连同自切割肽序列，而不破坏病毒动力学动力学。
在腺病毒质粒中的外源基因的插入受到衣壳大小容量的限制。外源核酸的过量添加导致不完整的基因组加载到所述衣壳中并降低病毒动力学。针对由腺病毒基因组中有限的可用空间呈现的挑战的解决方案是，将外源开放阅读框(ORF)定位在天然腺病毒ORF内作为融合产物。该策略利用已经在基因组中编码的腺病毒启动子、5'UTR和polyA尾巴。然而，天然腺病毒蛋白和外源蛋白之间融合的表达可能对一种或两种蛋白质功能都有害，并导致腺病毒复制动力学的显著降低。事实上，本文公开的研究证明了外源ORF直接融合至所述腺病毒E1A、DNA聚合酶或ADP ORF显著抑制腺病毒复制动力学。此外，发明人之前尝试使用内部核糖体进入位点(IRES)来插入外源ORF，其也不能产生具有野生型动力学的重组病毒。
该实施例通过使用置于所述天然ORF和所述外源ORF之间的自切割肽序列描述了对该问题的解决方案。当置于单个mRNA上的所述两个ORF之间时，所述自切割肽序列的存在导致核糖体跳过并释放与第二蛋白分离的第一蛋白。在该实施例中生成的病毒构建体使用自切割肽P2A和荧光蛋白(例如YPet，m樱桃红(mCherry))作为异源ORF。
以下表格提供生成的构建体的列表，且表明在两种不同的细胞系(293-E4细胞和A549细胞)中，外源ORF的表达水平(低、中或高)和病毒复制动力学的水平(低、中或高)。
在两种细胞类型中都表现出“高”复制动力学(即，与野生型腺病毒相比的复制动力学)的构建体被认为是生成治疗性腺病毒构建体的候选物(粗体显示)。另外，纤维蛋白-P2A-异源ORF(fiber-P2A-异源ORF)构建体表现出了显著的病毒复制缺陷，但生成非常高的异源蛋白表达水平。因此，这些纤维蛋白构建体也用于在有复制缺陷的腺病毒的背景下产生高水平的治疗性蛋白的治疗性应用。
P2A位点的直接融合和插入的比较
生成了一些构建体，其中将荧光蛋白直接融合至腺病毒ORF。具体地，生成了以下直接融合：YPet-E1A、YPet-(DNA聚合酶)和mCherry-ADP。
YPet-E1A腺病毒表现出了在病毒动力学中的显著损伤。在YPet和E1A之间的P2A位点的插入(YPet-P2A-E1A)改善了病毒动力学，但没有将病毒动力学恢复至野生型水平。之后生成了另一个构建体以测试P2A和YPet与E1A的C末端的融合(E1A-P2A-YPet)。该构建体进一步改善了病毒动力学，但再一次没有将动力学恢复至野生型腺病毒的水平。
多次尝试转染所述YPet-(DNA-poly)基因组质粒都未能产生活病毒(没有斑块形成)。然而，如上表所示，YPet-P2A与DNA聚合酶N末端的融合(YPet-P2A-(DNA poly))产生了具有野生型动力学的病毒。
此外，mCherry与ADP的直接融合(mCherry-ADP)产生了具有显著受损的动力学的病毒。然而，在所述mCherryORF和所述ADP ORF之间的所述P2A位点的插入导致了具有野生型动力学的病毒(mCherry-P2A-ADP)。使用不同的荧光蛋白获得了相同的结果；所述YPet-P2A-ADP构建体表现出了野生型病毒动力学。然而，将P2A和所述异源ORF放置在ADP的C末端产生了不复制的病毒。因此，对于ADP，所述异源ORF必须被放置在N末端。
与纤维蛋白(fiber)的直接融合以及在纤维蛋白和异源ORF之间的P2A位点的插入都产生了具有显著受损的复制动力学的病毒。然而，所述包括纤维蛋白-P2A-异源ORF的重组腺病毒表现出所述异源蛋白的非常高的表达水平(在此实施例中YPet或绿色荧光蛋白(BFP))。
具有野生型病毒动力学的额外的构建体
图7显示六种不同构建体：YPet-E1A、YPet-P2A-E1A、E1A-P2A-mCherry、E1B-55k-P2A-YPet、YPet-P2A-ADP和Fiber-P2A-YPet的Ln-斜率的比较。如上文所讨论的，YPet与E1A的直接融合产生了具有显著受损的动力学的病毒，并且在N末端(YPet-P2A-E1A)或C末端添加P2A位点(E1A-P2A-mCherry)改善了病毒动力学但没有达到野生型水平。然而，将所述P2A位点和异源ORF插入E1B-55k的C末端(E1B-55k-P2A-YPet)或ADP的N-末端(YPet-P2A-ADP)生成具有野生型病毒动力学的重组病毒。
评价在DBP的C末端或E3-14.7k的C末端具有P2A位点和异源ORF的构建体(分别为DBP-P2A-YPet或E3-14.7k-P2A-YPet)，或在E4-ORF2的N-末端具有P2A位点和异源ORF的构建体(YPet-P2A-E4-ORF2和mCherry-P2A-E4-ORF2)的病毒动力学，产生具有野生型复制动力学的病毒。
这些数据的结果表明，至少以下腺病毒基因组构建体可用于开发治疗性腺病毒构建体：
E1B-55k-P2A-异源ORF
异源ORF-P2A-(DNA聚合酶)
DBP-SC-异源ORF
异源ORF-SC-ADP
E3-14.7k-SC-异源ORF
异源ORF-P2A-E4-ORF2
纤维蛋白-P2A-异源ORF
针对治疗性应用，所述异源ORF编码治疗性蛋白。
其他腺病毒血清型
先前描述的测量病毒动力学的方法都高度依赖于细胞类型特异性测定，因此由于每种腺病毒血清型的不同趋向性而具有血清型特异性。本文公开的所述腺病毒动力学测定不依赖于任何一种细胞类型，且因此可以扩展到除Ad5之外的血清型。所有腺病毒血清型都含有相当于Ad5E3-14.7k的ORF。因此，使用Ad9(含有E3-15k)和Ad34(含有E3-14.8k)生成相当于Ad5E3-14.7k-P2A-YPet(PCMN-887；SEQ ID NO:9)的病毒：PCMN-888(Ad9E3-15k-P2A-YPet；SEQ ID NO:22)和PCMN-889(Ad34E3-14.8k-P2A-YPet；SEQ ID NO:23)。还生成了含有所述Ad5核心和来自Ad9或Ad34的纤维轴和球突的嵌合病毒。然后使用293细胞(图9A)、A549细胞(图9B)和U2OS细胞(图9C)在FBVK测定中测试四种重组病毒。所有四种重组病毒都表现出高水平的YPet表达，因插入外源ORF而导致的病毒动力学最小的影响。
实施例2：评价腺病毒复制动力学的方法
用于组装重组腺病毒的Adsembly和AdSLIC方法提供用于在短期内生成大量重组腺病毒基因组和病毒的方法。然而，存在用于评价为临床和治疗性用途而设计的重组腺病毒复制动力学的快速和高通量的方法的需求。该实施例描述基于荧光的病毒动力学测定，其可用于测试重组腺病毒的病毒复制动力学(图3)。所述测定可用重组腺病毒基因组质粒或重组腺病毒粒子作为起始材料进行。
当用重组腺病毒基因组起始时，所述测定包括用腺病毒基因组质粒转染细胞(诸如在实施例1中描述的那些)以及随时间监控荧光团表达(图4A-4B)。选择转染条件以致最初约5-10％的细胞被转染。最初未转染的细胞可通过初始转染产生的病毒粒子进行二次感染。对数-斜率用作基于二级、三级和四级(等)感染的动力学测量，因此不必知道最初转染的细胞的百分比。图4A和4B显示用重组腺病毒基因组质粒开始的示例性的基于病毒的动力学测定。在此实施例中，使用48孔板，其允许同时测试14种不同的病毒构建体(一式三份)。所述48孔板的上半部分(图4A)包括6种不同病毒的一式三份的孔、3个模拟-感染的孔和3个具有FLUORESBRITETM珠子的“空白”孔，其补偿工具灵敏度漂移。所述48孔板下半部分(图4B)包括用8种不同病毒构建体的一式三份的孔。一旦细胞被转染，将所述板放置在TECANTM读板仪中以进行连续的荧光监测。收集的数据用于计算每一个构建体的ln-斜率(图8)。
所述测定也可以通过用重组病毒粒子感染细胞来进行。在此测定版本中，用重组病毒粒子感染细胞，并随时间监测荧光团表达(图5)。与用所述基因组质粒测定的版本一样，不必知道起始病毒群的精确滴度。通常，稀释系列用于初始感染，诸如从1:100至1:218,700的范围的稀释系列，如图5所示。1:100的稀释通常导致所有细胞的感染，而1:218,700的稀释通常导致极少数细胞的初始感染。在此实施例中，使用96孔板，并在8种不同的稀释(1:100、1:300、1:900、1:2700、1:8100、1:24,300、1:72,900和1:218,700)下同时测试11种不同的病毒构建体。所述板还包括4个模拟感染细胞的孔和4个FLUORESBRITETM珠子的孔。一旦所述细胞被感染，将所述板置于TECANTM读板仪中以进行连续荧光监测。收集的数据用于计算每个构建体的ln-斜率(图8)。
TECANTM读板仪还提供温育功能(保持适当的温度以及CO2和O2水平)。每15分钟采集数据点以计算所述ln-斜率。使用这些方法，有可能快速有效地比较许多不同病毒之间和不同细胞类型之间的动力学。例如，为了评估具体的重组腺病毒是否可以作为溶瘤病毒在治疗上使用，此分析可用于发现在肿瘤细胞中表现出高复制动力学而在非肿瘤细胞中表现慢病毒动力学的病毒。此外，所述重组病毒的病毒动力学可通过在该测定中感染或转染感兴趣的肿瘤细胞类型来评估。
计算对数-斜率(log-slope)
为了测量对数-斜率，通过取每个时间点测量的荧光强度的自然对数，将荧光强度与时间的线性图转换为半对数图。由于所述荧光强度在病毒复制期间表现出指数增长，因此当绘制ln(荧光强度)对时间时，该转换产生直线。然后使用标准最小二乘法拟合该直线。由此拟合产生的斜率是荧光对时间的ln-斜率，且因此是病毒生长对时间的ln-斜率。方程式如下所示。
其中FI是测量的荧光强度，t是时间，F0是在时间＝t0的初始荧光强度，且α是ln-斜率。
取两边的自然对数：
右手侧现在是具有ln-斜率为α的线性方程式。
鉴于可以应用所公开发明的原理的许多可能的实施方案，应该认识到，所示实施方案仅是本公开的示例，并且不应被视为限制本发明的范围。相反，本发明的范围由以下权利要求限定。因此，我们要求将这些权利要求的范围和精神内的所有内容作为我们的发明。
序列表
<110> 萨克生物研究学院
<120> 对病毒动力学影响最小的治疗性腺病毒中的外源基因表达
<130> 7158-96335-02
<150> US 62/298,653
<151> 2016-02-23
<160> 23
<170> PatentIn版本3.5
<210> 1
<211> 36791
<212> DNA
<213> 人工序列
<220>
<223> 合成腺病毒基因组YPet-P2A-E1A
<400> 1
catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60
ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120
gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180
gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240
taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300
agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360
gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420
cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480
tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540
tccgacaccg ggactgaaaa tggtgagcaa aggcgaagag ctgttcaccg gcgtggtgcc 600
catcctggtg gagctggacg gcgacgtgaa cggccacaag ttcagcgtga gcggcgaggg 660
cgagggcgac gccacctacg gcaagctgac cctgaagctg ctgtgcacca ccggcaagct 720
gcccgtgccc tggcccaccc tggtgaccac cctgggctac ggcgtgcagt gcttcgcccg 780
gtaccccgac cacatgaagc agcacgactt cttcaagagc gccatgcccg agggctacgt 840
gcaggagcgg accatcttct tcaaggacga cggcaactac aagacccggg ccgaggtgaa 900
gttcgagggc gacaccctgg tgaaccggat cgagctgaag ggcatcgact tcaaggagga 960
cggcaacatc ctgggccaca agctggagta caactacaac agccacaacg tgtacatcac 1020
cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccggcaca acatcgagga 1080
cggcggcgtg cagctggccg accactacca gcagaacacc cccatcggcg acggccccgt 1140
gctgctgccc gacaaccact acctgagcta ccagagcgcc ctgttcaagg accccaacga 1200
gaagcgggac cacatggtgc tgctggagtt cctgaccgcc gccggcatca ccgagggcat 1260
gaacgagctc tataagggaa gcggagctac taacttcagc ctgctgaagc aggctggtga 1320
cgtcgaggag aatcctggcc caatgagaca tattatctgc cacggaggtg ttattaccga 1380
agaaatggcc gccagtcttt tggaccagct gatcgaagag gtactggctg ataatcttcc 1440
acctcctagc cattttgaac cacctaccct tcacgaactg tatgatttag acgtgacggc 1500
ccccgaagat cccaacgagg aggcggtttc gcagattttt cccgactctg taatgttggc 1560
ggtgcaggaa gggattgact tactcacttt tccgccggcg cccggttctc cggagccgcc 1620
tcacctttcc cggcagcccg agcagccgga gcagagagcc ttgggtccgg tttctatgcc 1680
aaaccttgta ccggaggtga tcgatcttac ctgccacgag gctggctttc cacccagtga 1740
cgacgaggat gaagagggtg aggagtttgt gttagattat gtggagcacc ccgggcacgg 1800
ttgcaggtct tgtcattatc accggaggaa tacgggggac ccagatatta tgtgttcgct 1860
ttgctatatg aggacctgtg gcatgtttgt ctacagtaag tgaaaattat gggcagtggg 1920
tgatagagtg gtgggtttgg tgtggtaatt ttttttttaa tttttacagt tttgtggttt 1980
aaagaatttt gtattgtgat ttttttaaaa ggtcctgtgt ctgaacctga gcctgagccc 2040
gagccagaac cggagcctgc aagacctacc cgccgtccta aaatggcgcc tgctatcctg 2100
agacgcccga catcacctgt gtctagagaa tgcaatagta gtacggatag ctgtgactcc 2160
ggtccttcta acacacctcc tgagatacac ccggtggtcc cgctgtgccc cattaaacca 2220
gttgccgtga gagttggtgg gcgtcgccag gctgtggaat gtatcgagga cttgcttaac 2280
gagcctgggc aacctttgga cttgagctgt aaacgcccca ggccataagg tgtaaacctg 2340
tgattgcgtg tgtggttaac gcctttgttt gctgaatgag ttgatgtaag tttaataaag 2400
ggtgagataa tgtttaactt gcatggcgtg ttaaatgggg cggggcttaa agggtatata 2460
atgcgccgtg ggctaatctt ggttacatct gacctcatgg aggcttggga gtgtttggaa 2520
gatttttctg ctgtgcgtaa cttgctggaa cagagctcta acagtacctc ttggttttgg 2580
aggtttctgt ggggctcatc ccaggcaaag ttagtctgca gaattaagga ggattacaag 2640
tgggaatttg aagagctttt gaaatcctgt ggtgagctgt ttgattcttt gaatctgggt 2700
caccaggcgc ttttccaaga gaaggtcatc aagactttgg atttttccac accggggcgc 2760
gctgcggctg ctgttgcttt tttgagtttt ataaaggata aatggagcga agaaacccat 2820
ctgagcgggg ggtacctgct ggattttctg gccatgcatc tgtggagagc ggttgtgaga 2880
cacaagaatc gcctgctact gttgtcttcc gtccgcccgg cgataatacc gacggaggag 2940
cagcagcagc agcaggagga agccaggcgg cggcggcagg agcagagccc atggaacccg 3000
agagccggcc tggaccctcg ggaatgaatg ttgtacaggt ggctgaactg tatccagaac 3060
tgagacgcat tttgacaatt acagaggatg ggcaggggct aaagggggta aagagggagc 3120
ggggggcttg tgaggctaca gaggaggcta ggaatctagc ttttagctta atgaccagac 3180
accgtcctga gtgtattact tttcaacaga tcaaggataa ttgcgctaat gagcttgatc 3240
tgctggcgca gaagtattcc atagagcagc tgaccactta ctggctgcag ccaggggatg 3300
attttgagga ggctattagg gtatatgcaa aggtggcact taggccagat tgcaagtaca 3360
agatcagcaa acttgtaaat atcaggaatt gttgctacat ttctgggaac ggggccgagg 3420
tggagataga tacggaggat agggtggcct ttagatgtag catgataaat atgtggccgg 3480
gggtgcttgg catggacggg gtggttatta tgaatgtaag gtttactggc cccaatttta 3540
gcggtacggt tttcctggcc aataccaacc ttatcctaca cggtgtaagc ttctatgggt 3600
ttaacaatac ctgtgtggaa gcctggaccg atgtaagggt tcggggctgt gccttttact 3660
gctgctggaa gggggtggtg tgtcgcccca aaagcagggc ttcaattaag aaatgcctct 3720
ttgaaaggtg taccttgggt atcctgtctg agggtaactc cagggtgcgc cacaatgtgg 3780
cctccgactg tggttgcttc atgctagtga aaagcgtggc tgtgattaag cataacatgg 3840
tatgtggcaa ctgcgaggac agggcctctc agatgctgac ctgctcggac ggcaactgtc 3900
acctgctgaa gaccattcac gtagccagcc actctcgcaa ggcctggcca gtgtttgagc 3960
ataacatact gacccgctgt tccttgcatt tgggtaacag gaggggggtg ttcctacctt 4020
accaatgcaa tttgagtcac actaagatat tgcttgagcc cgagagcatg tccaaggtga 4080
acctgaacgg ggtgtttgac atgaccatga agatctggaa ggtgctgagg tacgatgaga 4140
cccgcaccag gtgcagaccc tgcgagtgtg gcggtaaaca tattaggaac cagcctgtga 4200
tgctggatgt gaccgaggag ctgaggcccg atcacttggt gctggcctgc acccgcgctg 4260
agtttggctc tagcgatgaa gatacagatt gaggtactga aatgtgtggg cgtggcttaa 4320
gggtgggaaa gaatatataa ggtgggggtc ttatgtagtt ttgtatctgt tttgcagcag 4380
ccgccgccgc ccaacttttc tatacaaagt tgtaatgagc accaactcgt ttgatggaag 4440
cattgtgagc tcatatttga caacgcgcat gcccccatgg gccggggtgc gtcagaatgt 4500
gatgggctcc agcattgatg gtcgccccgt cctgcccgca aactctacta ccttgaccta 4560
cgagaccgtg tctggaacgc cgttggagac tgcagcctcc gccgccgctt cagccgctgc 4620
agccaccgcc cgcgggattg tgactgactt tgctttcctg agcccgcttg caagcagtgc 4680
agcttcccgt tcatccgccc gcgatgacaa gttgacggct cttttggcac aattggattc 4740
tttgacccgg gaacttaatg tcgtttctca gcagctgttg gatctgcgcc agcaggtttc 4800
tgccctgaag gcttcctccc ctcccaatgc ggtttaaaac ataaataaaa aaccagactc 4860
tgtttggatt tggatcaagc ataagtgtct tgctgtcttt atttaggggt tttgcgcgcg 4920
cggtaggccc gggaccagcg gtctcggtcg ttgagggtcc tgtgtatttt ttccaggacg 4980
tggtaaaggt gactctggat gttcagatac atgggcataa gcccgtctct ggggtggagg 5040
tagcaccact gcagagcttc atgctgcggg gtggtgttgt agatgatcca gtcgtagcag 5100
gagcgctggg cgtggtgcct aaaaatgtct ttcagtagca agctgattgc caggggcagg 5160
cccttggtgt aagtgtttac aaagcggtta agctgggatg ggtgcatacg tggggatatg 5220
agatgcatct tggactgtat ttttaggttg gctatgttcc cagccatatc cctccgggga 5280
ttcatgttgt gcagaaccac cagcacagtg tatccggtgc acttgggaaa tttgtcatgt 5340
agcttagaag gaaatgcgtg gaagaacttg gagacgccct tgtgacctcc aagattttcc 5400
atgcattcgt ccataatgat ggcaatgggc ccacgggcgg cggcctgggc gaagatattt 5460
ctgggatcac taacgtcata gttgtgttcc aggatgagat cgtcataggc catttttaca 5520
aagcgcgggc ggagggtgcc agactgcggt ataatggttc catccggccc aggggcgtag 5580
ttaccctcac agatttgcat ttcccacgct ttgagttcag atggggggat catgtctacc 5640
tgcggggcga tgaagaaaac ggtttccggg gtaggggaga tcagctggga agaaagcagg 5700
ttcctgagca gctgcgactt accgcagccg gtgggcccgt aaatcacacc tattaccggg 5760
tgcaactggt agttaagaga gctgcagctg ccgtcatccc tgagcagggg ggccacttcg 5820
ttaagcatgt ccctgactcg catgttttcc ctgaccaaat ccgccagaag gcgctcgccg 5880
cccagcgata gcagttcttg caaggaagca aagtttttca acggtttgag accgtccgcc 5940
gtaggcatgc ttttgagcgt ttgaccaagc agttccaggc ggtcccacag ctcggtcacc 6000
tgctctacgg catctcgatc cagcatatct cctcgtttcg cgggttgggg cggctttcgc 6060
tgtacggcag tagtcggtgc tcgtccagac gggccagggt catgtctttc cacgggcgca 6120
gggtcctcgt cagcgtagtc tgggtcacgg tgaaggggtg cgctccgggc tgcgcgctgg 6180
ccagggtgcg cttgaggctg gtcctgctgg tgctgaagcg ctgccggtct tcgccctgcg 6240
cgtcggccag gtagcatttg accatggtgt catagtccag cccctccgcg gcgtggccct 6300
tggcgcgcag cttgcccttg gaggaggcgc cgcacgaggg gcagtgcaga cttttgaggg 6360
cgtagagctt gggcgcgaga aataccgatt ccggggagta ggcatccgcg ccgcaggccc 6420
cgcagacggt ctcgcattcc acgagccagg tgagctctgg ccgttcgggg tcaaaaacca 6480
ggtttccccc atgctttttg atgcgtttct tacctctggt ttccatgagc cggtgtccac 6540
gctcggtgac gaaaaggctg tccgtgtccc cgtatacaga cttgagaggc ctgtcctcga 6600
gcggtgttcc gcggtcctcc tcgtatagaa actcggacca ctctgagaca aaggctcgcg 6660
tccaggccag cacgaaggag gctaagtggg aggggtagcg gtcgttgtcc actagggggt 6720
ccactcgctc cagggtgtga agacacatgt cgccctcttc ggcatcaagg aaggtgattg 6780
gtttgtaggt gtaggccacg tgaccgggtg ttcctgaagg ggggctataa aagggggtgg 6840
gggcgcgttc gtcctcactc tcttccgcat cgctgtctgc gagggccagc tgttggggtg 6900
agtactccct ctgaaaagcg ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg 6960
aggaggattt gatattcacc tggcccgcgg tgatgccttt gagggtggcc gcatccatct 7020
ggtcagaaaa gacaatcttt ttgttgtcaa gcttggtggc aaacgacccg tagagggcgt 7080
tggacagcaa cttggcgatg gagcgcaggg tttggttttt gtcgcgatcg gcgcgctcct 7140
tggccgcgat gtttagctgc acgtattcgc gcgcaacgca ccgccattcg ggaaagacgg 7200
tggtgcgctc gtcgggcacc aggtgcacgc gccaaccgcg gttgtgcagg gtgacaaggt 7260
caacgctggt ggctacctct ccgcgtaggc gctcgttggt ccagcagagg cggccgccct 7320
tgcgcgagca gaatggcggt agggggtcta gctgcgtctc gtccgggggg tctgcgtcca 7380
cggtaaagac cccgggcagc aggcgcgcgt cgaagtagtc tatcttgcat ccttgcaagt 7440
ctagcgcctg ctgccatgcg cgggcggcaa gcgcgcgctc gtatgggttg agtgggggac 7500
cccatggcat ggggtgggtg agcgcggagg cgtacatgcc gcaaatgtcg taaacgtaga 7560
ggggctctct gagtattcca agatatgtag ggtagcatct tccaccgcgg atgctggcgc 7620
gcacgtaatc gtatagttcg tgcgagggag cgaggaggtc gggaccgagg ttgctacggg 7680
cgggctgctc tgctcggaag actatctgcc tgaagatggc atgtgagttg gatgatatgg 7740
ttggacgctg gaagacgttg aagctggcgt ctgtgagacc taccgcgtca cgcacgaagg 7800
aggcgtagga gtcgcgcagc ttgttgacca gctcggcggt gacctgcacg tctagggcgc 7860
agtagtccag ggtttccttg atgatgtcat acttatcctg tccctttttt ttccacagct 7920
cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg 7980
cctccgaacg gtaagagcct agcatgtaga actggttgac ggcctggtag gcgcagcatc 8040
ccttttctac gggtagcgcg tatgcctgcg cggccttccg gagcgaggtg tgggtgagcg 8100
caaaggtgtc cctgaccatg actttgaggt actggtattt gaagtcagtg tcgtcgcatc 8160
cgccctgctc ccagagcaaa aagtccgtgc gctttttgga acgcggattt ggcagggcga 8220
aggtgacatc gttgaagagt atctttcccg cgcgaggcat aaagttgcgt gtgatgcgga 8280
agggtcccgg cacctcggaa cggttgttaa ttacctgggc ggcgagcacg atctcgtcaa 8340
agccgttgat gttgtggccc acaatgtaaa gttccaagaa gcgcgggatg cccttgatgg 8400
aaggcaattt tttaagttcc tcgtaggtga gctcttcagg ggagctgagc ccgtgctctg 8460
aaagggccca gtctgcaaga tgagggttgg aagcgacgaa tgagctccac aggtcacggg 8520
ccattagcat ttgcaggtgg tcgcgaaagg tcctaaactg gcgacctatg gccatttttt 8580
ctggggtgat gcagtagaag gtaagcgggt cttgttccca gcggtcccat ccaaggttcg 8640
cggctaggtc tcgcgcggca gtcactagag gctcatctcc gccgaacttc atgaccagca 8700
tgaagggcac gagctgcttc ccaaaggccc ccatccaagt ataggtctct acatcgtagg 8760
tgacaaagag acgctcggtg cgaggatgcg agccgatcgg gaagaactgg atctcccgcc 8820
accaattgga ggagtggcta ttgatgtggt gaaagtagaa gtccctgcga cgggccgaac 8880
actcgtgctg gcttttgtaa aaacgtgcgc agtactggca gcggtgcacg ggctgtacat 8940
cctgcacgag gttgacctga cgaccgcgca caaggaagca gagtgggaat ttgagcccct 9000
cgcctggcgg gtttggctgg tggtcttcta cttcggctgc ttgtccttga ccgtctggct 9060
gctcgagggg agttacggtg gatcggacca ccacgccgcg cgagcccaaa gtccagatgt 9120
ccgcgcgcgg cggtcggagc ttgatgacaa catcgcgcag atgggagctg tccatggtct 9180
ggagctcccg cggcgtcagg tcaggcggga gctcctgcag gtttacctcg catagacggg 9240
tcagggcgcg ggctagatcc aggtgatacc taatttccag gggctggttg gtggcggcgt 9300
cgatggcttg caagaggccg catccccgcg gcgcgactac ggtaccgcgc ggcgggcggt 9360
gggccgcggg ggtgtccttg gatgatgcat ctaaaagcgg tgacgcgggc gagcccccgg 9420
aggtaggggg ggctccggac ccgccgggag agggggcagg ggcacgtcgg cgccgcgcgc 9480
gggcaggagc tggtgctgcg cgcgtaggtt gctggcgaac gcgacgacgc ggcggttgat 9540
ctcctgaatc tggcgcctct gcgtgaagac gacgggcccg gtgagcttga gcctgaaaga 9600
gagttcgaca gaatcaattt cggtgtcgtt gacggcggcc tggcgcaaaa tctcctgcac 9660
gtctcctgag ttgtcttgat aggcgatctc ggccatgaac tgctcgatct cttcctcctg 9720
gagatctccg cgtccggctc gctccacggt ggcggcgagg tcgttggaaa tgcgggccat 9780
gagctgcgag aaggcgttga ggcctccctc gttccagacg cggctgtaga ccacgccccc 9840
ttcggcatcg cgggcgcgca tgaccacctg cgcgagattg agctccacgt gccgggcgaa 9900
gacggcgtag tttcgcaggc gctgaaagag gtagttgagg gtggtggcgg tgtgttctgc 9960
cacgaagaag tacataaccc agcgtcgcaa cgtggattcg ttgatatccc ccaaggcctc 10020
aaggcgctcc atggcctcgt agaagtccac ggcgaagttg aaaaactggg agttgcgcgc 10080
cgacacggtt aactcctcct ccagaagacg gatgagctcg gcgacagtgt cgcgcacctc 10140
gcgctcaaag gctacagggg cctcttcttc ttcttcaatc tcctcttcca taagggcctc 10200
cccttcttct tcttctggcg gcggtggggg aggggggaca cggcggcgac gacggcgcac 10260
cgggaggcgg tcgacaaagc gctcgatcat ctccccgcgg cgacggcgca tggtctcggt 10320
gacggcgcgg ccgttctcgc gggggcgcag ttggaagacg ccgcccgtca tgtcccggtt 10380
atgggttggc ggggggctgc catgcggcag ggatacggcg ctaacgatgc atctcaacaa 10440
ttgttgtgta ggtactccgc cgccgaggga cctgagcgag tccgcatcga ccggatcgga 10500
aaacctctcg agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc 10560
gggcggcagc gggcggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt 10620
aaagtaggcg gtcttgagac ggcggatggt cgacagaagc accatgtcct tgggtccggc 10680
ctgctgaatg cgcaggcggt cggccatgcc ccaggcttcg ttttgacatc ggcgcaggtc 10740
tttgtagtag tcttgcatga gcctttctac cggcacttct tcttctcctt cctcttgtcc 10800
tgcatctctt gcatctatcg ctgcggcggc ggcggagttt ggccgtaggt ggcgccctct 10860
tcctcccatg cgtgtgaccc cgaagcccct catcggctga agcagggcta ggtcggcgac 10920
aacgcgctcg gctaatatgg cctgctgcac ctgcgtgagg gtagactgga agtcatccat 10980
gtccacaaag cggtggtatg cgcccgtgtt gatggtgtaa gtgcagttgg ccataacgga 11040
ccagttaacg gtctggtgac ccggctgcga gagctcggtg tacctgagac gcgagtaagc 11100
cctcgagtca aatacgtagt cgttgcaagt ccgcaccagg tactggtatc ccaccaaaaa 11160
gtgcggcggc ggctggcggt agaggggcca gcgtagggtg gccggggctc cgggggcgag 11220
atcttccaac ataaggcgat gatatccgta gatgtacctg gacatccagg tgatgccggc 11280
ggcggtggtg gaggcgcgcg gaaagtcgcg gacgcggttc cagatgttgc gcagcggcaa 11340
aaagtgctcc atggtcggga cgctctggcc ggtcaggcgc gcgcaatcgt tgacgctcta 11400
gaccgtgcaa aaggagagcc tgtaagcggg cactcttccg tggtctggtg gataaattcg 11460
caagggtatc atggcggacg accggggttc gagccccgta tccggccgtc cgccgtgatc 11520
catgcggtta ccgcccgcgt gtcgaaccca ggtgtgcgac gtcagacaac gggggagtgc 11580
tccttttggc ttccttccag gcgcggcggc tgctgcgcta gcttttttgg ccactggccg 11640
cgcgcagcgt aagcggttag gctggaaagc gaaagcatta agtggctcgc tccctgtagc 11700
cggagggtta ttttccaagg gttgagtcgc gggacccccg gttcgagtct cggaccggcc 11760
ggactgcggc gaacgggggt ttgcctcccc gtcatgcaag accccgcttg caaattcctc 11820
cggaaacagg gacgagcccc ttttttgctt ttcccagatg catccggtgc tgcggcagat 11880
gcgcccccct cctcagcagc ggcaagagca agagcagcgg cagacatgca gggcaccctc 11940
ccctcctcct accgcgtcag gaggggcgac atccgcggtt gacgcggcag cagatggtga 12000
ttacgaaccc ccgcggcgcc gggcccggca ctacctggac ttggaggagg gcgagggcct 12060
ggcgcggcta ggagcgccct ctcctgagcg gtacccaagg gtgcagctga agcgtgatac 12120
gcgtgaggcg tacgtgccgc ggcagaacct gtttcgcgac cgcgagggag aggagcccga 12180
ggagatgcgg gatcgaaagt tccacgcagg gcgcgagctg cggcatggcc tgaatcgcga 12240
gcggttgctg cgcgaggagg actttgagcc cgacgcgcga accgggatta gtcccgcgcg 12300
cgcacacgtg gcggccgccg acctggtaac cgcatacgag cagacggtga accaggagat 12360
taactttcaa aaaagcttta acaaccacgt gcgtacgctt gtggcgcgcg aggaggtggc 12420
tataggactg atgcatctgt gggactttgt aagcgcgctg gagcaaaacc caaatagcaa 12480
gccgctcatg gcgcagctgt tccttatagt gcagcacagc agggacaacg aggcattcag 12540
ggatgcgctg ctaaacatag tagagcccga gggccgctgg ctgctcgatt tgataaacat 12600
cctgcagagc atagtggtgc aggagcgcag cttgagcctg gctgacaagg tggccgccat 12660
caactattcc atgcttagcc tgggcaagtt ttacgcccgc aagatatacc atacccctta 12720
cgttcccata gacaaggagg taaagatcga ggggttctac atgcgcatgg cgctgaaggt 12780
gcttaccttg agcgacgacc tgggcgttta tcgcaacgag cgcatccaca aggccgtgag 12840
cgtgagccgg cggcgcgagc tcagcgaccg cgagctgatg cacagcctgc aaagggccct 12900
ggctggcacg ggcagcggcg atagagaggc cgagtcctac tttgacgcgg gcgctgacct 12960
gcgctgggcc ccaagccgac gcgccctgga ggcagctggg gccggacctg ggctggcggt 13020
ggcacccgcg cgcgctggca acgtcggcgg cgtggaggaa tatgacgagg acgatgagta 13080
cgagccagag gacggcgagt actaagcggt gatgtttctg atcagatgat gcaagacgca 13140
acggacccgg cggtgcgggc ggcgctgcag agccagccgt ccggccttaa ctccacggac 13200
gactggcgcc aggtcatgga ccgcatcatg tcgctgactg cgcgcaatcc tgacgcgttc 13260
cggcagcagc cgcaggccaa ccggctctcc gcaattctgg aagcggtggt cccggcgcgc 13320
gcaaacccca cgcacgagaa ggtgctggcg atcgtaaacg cgctggccga aaacagggcc 13380
atccggcccg acgaggccgg cctggtctac gacgcgctgc ttcagcgcgt ggctcgttac 13440
aacagcggca acgtgcagac caacctggac cggctggtgg gggatgtgcg cgaggccgtg 13500
gcgcagcgtg agcgcgcgca gcagcagggc aacctgggct ccatggttgc actaaacgcc 13560
ttcctgagta cacagcccgc caacgtgccg cggggacagg aggactacac caactttgtg 13620
agcgcactgc ggctaatggt gactgagaca ccgcaaagtg aggtgtacca gtctgggcca 13680
gactattttt tccagaccag tagacaaggc ctgcagaccg taaacctgag ccaggctttc 13740
aaaaacttgc aggggctgtg gggggtgcgg gctcccacag gcgaccgcgc gaccgtgtct 13800
agcttgctga cgcccaactc gcgcctgttg ctgctgctaa tagcgccctt cacggacagt 13860
ggcagcgtgt cccgggacac atacctaggt cacttgctga cactgtaccg cgaggccata 13920
ggtcaggcgc atgtggacga gcatactttc caggagatta caagtgtcag ccgcgcgctg 13980
gggcaggagg acacgggcag cctggaggca accctaaact acctgctgac caaccggcgg 14040
cagaagatcc cctcgttgca cagtttaaac agcgaggagg agcgcatttt gcgctacgtg 14100
cagcagagcg tgagccttaa cctgatgcgc gacggggtaa cgcccagcgt ggcgctggac 14160
atgaccgcgc gcaacatgga accgggcatg tatgcctcaa accggccgtt tatcaaccgc 14220
ctaatggact acttgcatcg cgcggccgcc gtgaaccccg agtatttcac caatgccatc 14280
ttgaacccgc actggctacc gccccctggt ttctacaccg ggggattcga ggtgcccgag 14340
ggtaacgatg gattcctctg ggacgacata gacgacagcg tgttttcccc gcaaccgcag 14400
accctgctag agttgcaaca gcgcgagcag gcagaggcgg cgctgcgaaa ggaaagcttc 14460
cgcaggccaa gcagcttgtc cgatctaggc gctgcggccc cgcggtcaga tgctagtagc 14520
ccatttccaa gcttgatagg gtctcttacc agcactcgca ccacccgccc gcgcctgctg 14580
ggcgaggagg agtacctaaa caactcgctg ctgcagccgc agcgcgaaaa aaacctgcct 14640
ccggcatttc ccaacaacgg gatagagagc ctagtggaca agatgagtag atggaagacg 14700
tacgcgcagg agcacaggga cgtgccaggc ccgcgcccgc ccacccgtcg tcaaaggcac 14760
gaccgtcagc ggggtctggt gtgggaggac gatgactcgg cagacgacag cagcgtcctg 14820
gatttgggag ggagtggcaa cccgtttgcg caccttcgcc ccaggctggg gagaatgttt 14880
taaaaaaaaa aaagcatgat gcaaaataaa aaactcacca aggccatggc accgagcgtt 14940
ggttttcttg tattcccctt agtatgcggc gcgcggcgat gtatgaggaa ggtcctcctc 15000
cctcctacga gagtgtggtg agcgcggcgc cagtggcggc ggcgctgggt tctcccttcg 15060
atgctcccct ggacccgccg tttgtgcctc cgcggtacct gcggcctacc ggggggagaa 15120
acagcatccg ttactctgag ttggcacccc tattcgacac cacccgtgtg tacctggtgg 15180
acaacaagtc aacggatgtg gcatccctga actaccagaa cgaccacagc aactttctga 15240
ccacggtcat tcaaaacaat gactacagcc cgggggaggc aagcacacag accatcaatc 15300
ttgacgaccg gtcgcactgg ggcggcgacc tgaaaaccat cctgcatacc aacatgccaa 15360
atgtgaacga gttcatgttt accaataagt ttaaggcgcg ggtgatggtg tcgcgcttgc 15420
ctactaagga caatcaggtg gagctgaaat acgagtgggt ggagttcacg ctgcccgagg 15480
gcaactactc cgagaccatg accatagacc ttatgaacaa cgcgatcgtg gagcactact 15540
tgaaagtggg cagacagaac ggggttctgg aaagcgacat cggggtaaag tttgacaccc 15600
gcaacttcag actggggttt gaccccgtca ctggtcttgt catgcctggg gtatatacaa 15660
acgaagcctt ccatccagac atcattttgc tgccaggatg cggggtggac ttcacccaca 15720
gccgcctgag caacttgttg ggcatccgca agcggcaacc cttccaggag ggctttagga 15780
tcacctacga tgatctggag ggtggtaaca ttcccgcact gttggatgtg gacgcctacc 15840
aggcgagctt gaaagatgac accgaacagg gcgggggtgg cgcaggcggc agcaacagca 15900
gtggcagcgg cgcggaagag aactccaacg cggcagccgc ggcaatgcag ccggtggagg 15960
acatgaacga tcatgccatt cgcggcgaca cctttgccac acgggctgag gagaagcgcg 16020
ctgaggccga agcagcggcc gaagctgccg cccccgctgc gcaacccgag gtcgagaagc 16080
ctcagaagaa accggtgatc aaacccctga cagaggacag caagaaacgc agttacaacc 16140
taataagcaa tgacagcacc ttcacccagt accgcagctg gtaccttgca tacaactacg 16200
gcgaccctca gaccggaatc cgctcatgga ccctgctttg cactcctgac gtaacctgcg 16260
gctcggagca ggtctactgg tcgttgccag acatgatgca agaccccgtg accttccgct 16320
ccacgcgcca gatcagcaac tttccggtgg tgggcgccga gctgttgccc gtgcactcca 16380
agagcttcta caacgaccag gccgtctact cccaactcat ccgccagttt acctctctga 16440
cccacgtgtt caatcgcttt cccgagaacc agattttggc gcgcccgcca gcccccacca 16500
tcaccaccgt cagtgaaaac gttcctgctc tcacagatca cgggacgcta ccgctgcgca 16560
acagcatcgg aggagtccag cgagtgacca ttactgacgc cagacgccgc acctgcccct 16620
acgtttacaa ggccctgggc atagtctcgc cgcgcgtcct atcgagccgc actttttgag 16680
caagcatgtc catccttata tcgcccagca ataacacagg ctggggcctg cgcttcccaa 16740
gcaagatgtt tggcggggcc aagaagcgct ccgaccaaca cccagtgcgc gtgcgcgggc 16800
actaccgcgc gccctggggc gcgcacaaac gcggccgcac tgggcgcacc accgtcgatg 16860
acgccatcga cgcggtggtg gaggaggcgc gcaactacac gcccacgccg ccaccagtgt 16920
ccacagtgga cgcggccatt cagaccgtgg tgcgcggagc ccggcgctat gctaaaatga 16980
agagacggcg gaggcgcgta gcacgtcgcc accgccgccg acccggcact gccgcccaac 17040
gcgcggcggc ggccctgctt aaccgcgcac gtcgcaccgg ccgacgggcg gccatgcggg 17100
ccgctcgaag gctggccgcg ggtattgtca ctgtgccccc caggtccagg cgacgagcgg 17160
ccgccgcagc agccgcggcc attagtgcta tgactcaggg tcgcaggggc aacgtgtatt 17220
gggtgcgcga ctcggttagc ggcctgcgcg tgcccgtgcg cacccgcccc ccgcgcaact 17280
agattgcaag aaaaaactac ttagactcgt actgttgtat gtatccagcg gcggcggcgc 17340
gcaacgaagc tatgtccaag cgcaaaatca aagaagagat gctccaggtc atcgcgccgg 17400
agatctatgg ccccccgaag aaggaagagc aggattacaa gccccgaaag ctaaagcggg 17460
tcaaaaagaa aaagaaagat gatgatgatg aacttgacga cgaggtggaa ctgctgcacg 17520
ctaccgcgcc caggcgacgg gtacagtgga aaggtcgacg cgtaaaacgt gttttgcgac 17580
ccggcaccac cgtagtcttt acgcccggtg agcgctccac ccgcacctac aagcgcgtgt 17640
atgatgaggt gtacggcgac gaggacctgc ttgagcaggc caacgagcgc ctcggggagt 17700
ttgcctacgg aaagcggcat aaggacatgc tggcgttgcc gctggacgag ggcaacccaa 17760
cacctagcct aaagcccgta acactgcagc aggtgctgcc cgcgcttgca ccgtccgaag 17820
aaaagcgcgg cctaaagcgc gagtctggtg acttggcacc caccgtgcag ctgatggtac 17880
ccaagcgcca gcgactggaa gatgtcttgg aaaaaatgac cgtggaacct gggctggagc 17940
ccgaggtccg cgtgcggcca atcaagcagg tggcgccggg actgggcgtg cagaccgtgg 18000
acgttcagat acccactacc agtagcacca gtattgccac cgccacagag ggcatggaga 18060
cacaaacgtc cccggttgcc tcagcggtgg cggatgccgc ggtgcaggcg gtcgctgcgg 18120
ccgcgtccaa gacctctacg gaggtgcaaa cggacccgtg gatgtttcgc gtttcagccc 18180
cccggcgccc gcgcggttcg aggaagtacg gcgccgccag cgcgctactg cccgaatatg 18240
ccctacatcc ttccattgcg cctacccccg gctatcgtgg ctacacctac cgccccagaa 18300
gacgagcaac tacccgacgc cgaaccacca ctggaacccg ccgccgccgt cgccgtcgcc 18360
agcccgtgct ggccccgatt tccgtgcgca gggtggctcg cgaaggaggc aggaccctgg 18420
tgctgccaac agcgcgctac caccccagca tcgtttaaaa gccggtcttt gtggttcttg 18480
cagatatggc cctcacctgc cgcctccgtt tcccggtgcc gggattccga ggaagaatgc 18540
accgtaggag gggcatggcc ggccacggcc tgacgggcgg catgcgtcgt gcgcaccacc 18600
ggcggcggcg cgcgtcgcac cgtcgcatgc gcggcggtat cctgcccctc cttattccac 18660
tgatcgccgc ggcgattggc gccgtgcccg gaattgcatc cgtggccttg caggcgcaga 18720
gacactgatt aaaaacaagt tgcatgtgga aaaatcaaaa taaaaagtct ggactctcac 18780
gctcgcttgg tcctgtaact attttgtaga atggaagaca tcaactttgc gtctctggcc 18840
ccgcgacacg gctcgcgccc gttcatggga aactggcaag atatcggcac cagcaatatg 18900
agcggtggcg ccttcagctg gggctcgctg tggagcggca ttaaaaattt cggttccacc 18960
gttaagaact atggcagcaa ggcctggaac agcagcacag gccagatgct gagggataag 19020
ttgaaagagc aaaatttcca acaaaaggtg gtagatggcc tggcctctgg cattagcggg 19080
gtggtggacc tggccaacca ggcagtgcaa aataagatta acagtaagct tgatccccgc 19140
cctcccgtag aggagcctcc accggccgtg gagacagtgt ctccagaggg gcgtggcgaa 19200
aagcgtccgc gccccgacag ggaagaaact ctggtgacgc aaatagacga gcctccctcg 19260
tacgaggagg cactaaagca aggcctgccc accacccgtc ccatcgcgcc catggctacc 19320
ggagtgctgg gccagcacac acccgtaacg ctggacctgc ctccccccgc cgacacccag 19380
cagaaacctg tgctgccagg cccgaccgcc gttgttgtaa cccgtcctag ccgcgcgtcc 19440
ctgcgccgcg ccgccagcgg tccgcgatcg ttgcggcccg tagccagtgg caactggcaa 19500
agcacactga acagcatcgt gggtctgggg gtgcaatccc tgaagcgccg acgatgcttc 19560
tgaatagcta acgtgtcgta tgtgtgtcat gtatgcgtcc atgtcgccgc cagaggagct 19620
gctgagccgc cgcgcgcccg ctttccaaga tggctacccc ttcgatgatg ccgcagtggt 19680
cttacatgca catctcgggc caggacgcct cggagtacct gagccccggg ctggtgcagt 19740
ttgcccgcgc caccgagacg tacttcagcc tgaataacaa gtttagaaac cccacggtgg 19800
cgcctacgca cgacgtgacc acagaccggt cccagcgttt gacgctgcgg ttcatccctg 19860
tggaccgtga ggatactgcg tactcgtaca aggcgcggtt caccctagct gtgggtgata 19920
accgtgtgct ggacatggct tccacgtact ttgacatccg cggcgtgctg gacaggggcc 19980
ctacttttaa gccctactct ggcactgcct acaacgccct ggctcccaag ggtgccccaa 20040
atccttgcga atgggatgaa gctgctactg ctcttgaaat aaacctagaa gaagaggacg 20100
atgacaacga agacgaagta gacgagcaag ctgagcagca aaaaactcac gtatttgggc 20160
aggcgcctta ttctggtata aatattacaa aggagggtat tcaaataggt gtcgaaggtc 20220
aaacacctaa atatgccgat aaaacatttc aacctgaacc tcaaatagga gaatctcagt 20280
ggtacgaaac tgaaattaat catgcagctg ggagagtcct taaaaagact accccaatga 20340
aaccatgtta cggttcatat gcaaaaccca caaatgaaaa tggagggcaa ggcattcttg 20400
taaagcaaca aaatggaaag ctagaaagtc aagtggaaat gcaatttttc tcaactactg 20460
aggcgaccgc aggcaatggt gataacttga ctcctaaagt ggtattgtac agtgaagatg 20520
tagatataga aaccccagac actcatattt cttacatgcc cactattaag gaaggtaact 20580
cacgagaact aatgggccaa caatctatgc ccaacaggcc taattacatt gcttttaggg 20640
acaattttat tggtctaatg tattacaaca gcacgggtaa tatgggtgtt ctggcgggcc 20700
aagcatcgca gttgaatgct gttgtagatt tgcaagacag aaacacagag ctttcatacc 20760
agcttttgct tgattccatt ggtgatagaa ccaggtactt ttctatgtgg aatcaggctg 20820
ttgacagcta tgatccagat gttagaatta ttgaaaatca tggaactgaa gatgaacttc 20880
caaattactg ctttccactg ggaggtgtga ttaatacaga gactcttacc aaggtaaaac 20940
ctaaaacagg tcaggaaaat ggatgggaaa aagatgctac agaattttca gataaaaatg 21000
aaataagagt tggaaataat tttgccatgg aaatcaatct aaatgccaac ctgtggagaa 21060
atttcctgta ctccaacata gcgctgtatt tgcccgacaa gctaaagtac agtccttcca 21120
acgtaaaaat ttctgataac ccaaacacct acgactacat gaacaagcga gtggtggctc 21180
ccgggttagt ggactgctac attaaccttg gagcacgctg gtcccttgac tatatggaca 21240
acgtcaaccc atttaaccac caccgcaatg ctggcctgcg ctaccgctca atgttgctgg 21300
gcaatggtcg ctatgtgccc ttccacatcc aggtgcctca gaagttcttt gccattaaaa 21360
acctccttct cctgccgggc tcatacacct acgagtggaa cttcaggaag gatgttaaca 21420
tggttctgca gagctcccta ggaaatgacc taagggttga cggagccagc attaagtttg 21480
atagcatttg cctttacgcc accttcttcc ccatggccca caacaccgcc tccacgcttg 21540
aggccatgct tagaaacgac accaacgacc agtcctttaa cgactatctc tccgccgcca 21600
acatgctcta ccctataccc gccaacgcta ccaacgtgcc catatccatc ccctcccgca 21660
actgggcggc tttccgcggc tgggccttca cgcgccttaa gactaaggaa accccatcac 21720
tgggctcggg ctacgaccct tattacacct actctggctc tataccctac ctagatggaa 21780
ccttttacct caaccacacc tttaagaagg tggccattac ctttgactct tctgtcagct 21840
ggcctggcaa tgaccgcctg cttaccccca acgagtttga aattaagcgc tcagttgacg 21900
gggagggtta caacgttgcc cagtgtaaca tgaccaaaga ctggttcctg gtacaaatgc 21960
tagctaacta caacattggc taccagggct tctatatccc agagagctac aaggaccgca 22020
tgtactcctt ctttagaaac ttccagccca tgagccgtca ggtggtggat gatactaaat 22080
acaaggacta ccaacaggtg ggcatcctac accaacacaa caactctgga tttgttggct 22140
accttgcccc caccatgcgc gaaggacagg cctaccctgc taacttcccc tatccgctta 22200
taggcaagac cgcagttgac agcattaccc agaaaaagtt tctttgcgat cgcacccttt 22260
ggcgcatccc attctccagt aactttatgt ccatgggcgc actcacagac ctgggccaaa 22320
accttctcta cgccaactcc gcccacgcgc tagacatgac ttttgaggtg gatcccatgg 22380
acgagcccac ccttctttat gttttgtttg aagtctttga cgtggtccgt gtgcaccggc 22440
cgcaccgcgg cgtcatcgaa accgtgtacc tgcgcacgcc cttctcggcc ggcaacgcca 22500
caacataaag aagcaagcaa catcaacaac agctgccgcc atgggctcca gtgagcagga 22560
actgaaagcc attgtcaaag atcttggttg tgggccatat tttttgggca cctatgacaa 22620
gcgctttcca ggctttgttt ctccacacaa gctcgcctgc gccatagtca atacggccgg 22680
tcgcgagact gggggcgtac actggatggc ctttgcctgg aacccgcact caaaaacatg 22740
ctacctcttt gagccctttg gcttttctga ccagcgactc aagcaggttt accagtttga 22800
gtacgagtca ctcctgcgcc gtagcgccat tgcttcttcc cccgaccgct gtataacgct 22860
ggaaaagtcc acccaaagcg tacaggggcc caactcggcc gcctgtggac tattctgctg 22920
catgtttctc cacgcctttg ccaactggcc ccaaactccc atggatcaca accccaccat 22980
gaaccttatt accggggtac ccaactccat gctcaacagt ccccaggtac agcccaccct 23040
gcgtcgcaac caggaacagc tctacagctt cctggagcgc cactcgccct acttccgcag 23100
ccacagtgcg cagattagga gcgccacttc tttttgtcac ttgaaaaaca tgtaaaaata 23160
atgtactaga gacactttca ataaaggcaa atgcttttat ttgtacactc tcgggtgatt 23220
atttaccccc acccttgccg tctgcgccgt ttaaaaatca aaggggttct gccgcgcatc 23280
gctatgcgcc actggcaggg acacgttgcg atactggtgt ttagtgctcc acttaaactc 23340
aggcacaacc atccgcggca gctcggtgaa gttttcactc cacaggctgc gcaccatcac 23400
caacgcgttt agcaggtcgg gcgccgatat cttgaagtcg cagttggggc ctccgccctg 23460
cgcgcgcgag ttgcgataca cagggttgca gcactggaac actatcagcg ccgggtggtg 23520
cacgctggcc agcacgctct tgtcggagat cagatccgcg tccaggtcct ccgcgttgct 23580
cagggcgaac ggagtcaact ttggtagctg ccttcccaaa aagggcgcgt gcccaggctt 23640
tgagttgcac tcgcaccgta gtggcatcaa aaggtgaccg tgcccggtct gggcgttagg 23700
atacagcgcc tgcataaaag ccttgatctg cttaaaagcc acctgagcct ttgcgccttc 23760
agagaagaac atgccgcaag acttgccgga aaactgattg gccggacagg ccgcgtcgtg 23820
cacgcagcac cttgcgtcgg tgttggagat ctgcaccaca tttcggcccc accggttctt 23880
cacgatcttg gccttgctag actgctcctt cagcgcgcgc tgcccgtttt cgctcgtcac 23940
atccatttca atcacgtgct ccttatttat cataatgctt ccgtgtagac acttaagctc 24000
gccttcgatc tcagcgcagc ggtgcagcca caacgcgcag cccgtgggct cgtgatgctt 24060
gtaggtcacc tctgcaaacg actgcaggta cgcctgcagg aatcgcccca tcatcgtcac 24120
aaaggtcttg ttgctggtga aggtcagctg caacccgcgg tgctcctcgt tcagccaggt 24180
cttgcatacg gccgccagag cttccacttg gtcaggcagt agtttgaagt tcgcctttag 24240
atcgttatcc acgtggtact tgtccatcag cgcgcgcgca gcctccatgc ccttctccca 24300
cgcagacacg atcggcacac tcagcgggtt catcaccgta atttcacttt ccgcttcgct 24360
gggctcttcc tcttcctctt gcgtccgcat accacgcgcc actgggtcgt cttcattcag 24420
ccgccgcact gtgcgcttac ctcctttgcc atgcttgatt agcaccggtg ggttgctgaa 24480
acccaccatt tgtagcgcca catcttctct ttcttcctcg ctgtccacga ttacctctgg 24540
tgatggcggg cgctcgggct tgggagaagg gcgcttcttt ttcttcttgg gcgcaatggc 24600
caaatccgcc gccgaggtcg atggccgcgg gctgggtgtg cgcggcacca gcgcgtcttg 24660
tgatgagtct tcctcgtcct cggactcgat acgccgcctc atccgctttt ttgggggcgc 24720
ccggggaggc ggcggcgacg gggacgggga cgacacgtcc tccatggttg ggggacgtcg 24780
cgccgcaccg cgtccgcgct cgggggtggt ttcgcgctgc tcctcttccc gactggccat 24840
ttccttctcc tataggcaga aaaagatcat ggagtcagtc gagaagaagg acagcctaac 24900
cgccccctct gagttcgcca ccaccgcctc caccgatgcc gccaacgcgc ctaccacctt 24960
ccccgtcgag gcacccccgc ttgaggagga ggaagtgatt atcgagcagg acccaggttt 25020
tgtaagcgaa gacgacgagg accgctcagt accaacagag gataaaaagc aagaccagga 25080
caacgcagag gcaaacgagg aacaagtcgg gcggggggac gaaaggcatg gcgactacct 25140
agatgtggga gacgacgtgc tgttgaagca tctgcagcgc cagtgcgcca ttatctgcga 25200
cgcgttgcaa gagcgcagcg atgtgcccct cgccatagcg gatgtcagcc ttgcctacga 25260
acgccaccta ttctcaccgc gcgtaccccc caaacgccaa gaaaacggca catgcgagcc 25320
caacccgcgc ctcaacttct accccgtatt tgccgtgcca gaggtgcttg ccacctatca 25380
catctttttc caaaactgca agatacccct atcctgccgt gccaaccgca gccgagcgga 25440
caagcagctg gccttgcggc agggcgctgt catacctgat atcgcctcgc tcaacgaagt 25500
gccaaaaatc tttgagggtc ttggacgcga cgagaagcgc gcggcaaacg ctctgcaaca 25560
ggaaaacagc gaaaatgaaa gtcactctgg agtgttggtg gaactcgagg gtgacaacgc 25620
gcgcctagcc gtactaaaac gcagcatcga ggtcacccac tttgcctacc cggcacttaa 25680
cctacccccc aaggtcatga gcacagtcat gagtgagctg atcgtgcgcc gtgcgcagcc 25740
cctggagagg gatgcaaatt tgcaagaaca aacagaggag ggcctacccg cagttggcga 25800
cgagcagcta gcgcgctggc ttcaaacgcg cgagcctgcc gacttggagg agcgacgcaa 25860
actaatgatg gccgcagtgc tcgttaccgt ggagcttgag tgcatgcagc ggttctttgc 25920
tgacccggag atgcagcgca agctagagga aacattgcac tacacctttc gacagggcta 25980
cgtacgccag gcctgcaaga tctccaacgt ggagctctgc aacctggtct cctaccttgg 26040
aattttgcac gaaaaccgcc ttgggcaaaa cgtgcttcat tccacgctca agggcgaggc 26100
gcgccgcgac tacgtccgcg actgcgttta cttatttcta tgctacacct ggcagacggc 26160
catgggcgtt tggcagcagt gcttggagga gtgcaacctc aaggagctgc agaaactgct 26220
aaagcaaaac ttgaaggacc tatggacggc cttcaacgag cgctccgtgg ccgcgcacct 26280
ggcggacatc attttccccg aacgcctgct taaaaccctg caacagggtc tgccagactt 26340
caccagtcaa agcatgttgc agaactttag gaactttatc ctagagcgct caggaatctt 26400
gcccgccacc tgctgtgcac ttcctagcga ctttgtgccc attaagtacc gcgaatgccc 26460
tccgccgctt tggggccact gctaccttct gcagctagcc aactaccttg cctaccactc 26520
tgacataatg gaagacgtga gcggtgacgg tctactggag tgtcactgtc gctgcaacct 26580
atgcaccccg caccgctccc tggtttgcaa ttcgcagctg cttaacgaaa gtcaaattat 26640
cggtaccttt gagctgcagg gtccctcgcc tgacgaaaag tccgcggctc cggggttgaa 26700
actcactccg gggctgtgga cgtcggctta ccttcgcaaa tttgtacctg aggactacca 26760
cgcccacgag attaggttct acgaagacca atcccgcccg ccaaatgcgg agcttaccgc 26820
ctgcgtcatt acccagggcc acattcttgg ccaattgcaa gccatcaaca aagcccgcca 26880
agagtttctg ctacgaaagg gacggggggt ttacttggac ccccagtccg gcgaggagct 26940
caacccaatc cccccgccgc cgcagcccta tcagcagcag ccgcgggccc ttgcttccca 27000
ggatggcacc caaaaagaag ctgcagctgc cgccgccacc cacggacgag gaggaatact 27060
gggacagtca ggcagaggag gttttggacg aggaggagga ggacatgatg gaagactggg 27120
agagcctaga cgaggaagct tccgaggtcg aagaggtgtc agacgaaaca ccgtcaccct 27180
cggtcgcatt cccctcgccg gcgccccaga aatcggcaac cggttccagc atggctacaa 27240
cctccgctcc tcaggcgccg ccggcactgc ccgttcgccg acccaaccgt agatgggaca 27300
ccactggaac cagggccggt aagtccaagc agccgccgcc gttagcccaa gagcaacaac 27360
agcgccaagg ctaccgctca tggcgcgggc acaagaacgc catagttgct tgcttgcaag 27420
actgtggggg caacatctcc ttcgcccgcc gctttcttct ctaccatcac ggcgtggcct 27480
tcccccgtaa catcctgcat tactaccgtc atctctacag cccatactgc accggcggca 27540
gcggcagcgg cagcaacagc agcggccaca cagaagcaaa ggcgaccgga tagcaagact 27600
ctgacaaagc ccaagaaatc cacagcggcg gcagcagcag gaggaggagc gctgcgtctg 27660
gcgcccaacg aacccgtatc gacccgcgag cttagaaaca ggatttttcc cactctgtat 27720
gctatatttc aacagagcag gggccaagaa caagagctga aaataaaaaa caggtctctg 27780
cgatccctca cccgcagctg cctgtatcac aaaagcgaag atcagcttcg gcgcacgctg 27840
gaagacgcgg aggctctctt cagtaaatac tgcgcgctga ctcttaagga ctagtttcgc 27900
gccctttctc aaatttaagc gcgaaaacta cgtcatctcc agcggccaca cccggcgcca 27960
gcacctgtcg tcagcgccat ttcaactttg tatacaaaag ttgtgatgag caaggaaatt 28020
cccacgccct acatgtggag ttaccagcca caaatgggac ttgcggctgg agctgcccaa 28080
gactactcaa cccgaataaa ctacatgagc gcgggacccc acatgatatc ccgggtcaac 28140
ggaatacgcg cccaccgaaa ccgaattctc ctggaacagg cggctattac caccacacct 28200
cgtaataacc ttaatccccg tagttggccc gctgccctgg tgtaccagga aagtcccgct 28260
cccaccactg tggtacttcc cagagacgcc caggccgaag ttcagatgac taactcaggg 28320
gcgcagcttg cgggcggctt tcgtcacagg gtgcggtcgc ccgggcaggg tataactcac 28380
ctgacaatca gagggcgagg tattcagctc aacgacgagt cggtgagctc ctcgcttggt 28440
ctccgtccgg acgggacatt tcagatcggc ggcgccggcc gctcttcatt cacgcctcgt 28500
caggcaatcc taactctgca gacctcgtcc tctgagccgc gctctggagg cattggaact 28560
ctgcaattta ttgaggagtt tgtgccatcg gtctacttta accccttctc gggacctccc 28620
ggccactatc cggatcaatt tattcctaac tttgacgcgg taaaggactc ggcggacggc 28680
tacgactgaa tgttaagtgg agaggcagag caactgcgcc tgaaacacct ggtccactgt 28740
cgccgccaca agtgctttgc ccgcgactcc ggtgagtttt gctactttga attgcccgag 28800
gatcatatcg agggcccggc gcacggcgtc cggcttaccg cccagggaga gcttgcccgt 28860
agcctgattc gggagtttac ccagcgcccc ctgctagttg agcgggacag gggaccctgt 28920
gttctcactg tgatttgcaa ctgtcctaac cctggattac atcaagatct ttgttgccat 28980
ctctgtgctg agtataataa atacagaaat taaaatatac tggggctcct atcgccatcc 29040
tgtaaacgcc accgtcttca cccgcccaag caaaccaagg cgaaccttac ctggtacttt 29100
taacatctct ccctctgtga tttacaacag tttcaaccca gacggagtga gtctacgaga 29160
gaacctctcc gagctcagct actccatcag aaaaaacacc accctcctta cctgccggga 29220
acgtacgagt gcgtcaccgg ccgctgcacc acacctaccg cctgaccgta aaccagactt 29280
tttccggaca gacctcaata actctgttta ccagaacagg aggtgagctt agaaaaccct 29340
tagggtatta ggccaaaggc gcagctactg tggggtttat gaacaattca agcaactcta 29400
cgggctattc taattcaggt ttctctagaa tcggggttgg ggttattctc tgtcttgtga 29460
ttctctttat tcttatacta acgcttctct gcctaaggct cgccgcctgc tgtgtgcaca 29520
tttgcattta ttgtcagctt tttaaacgct ggggtcgcca cccaagatga ttaggtacat 29580
aatcctaggt ttactcaccc ttgcgtcagc ccacggtacc acccaaaagg tggattttaa 29640
ggagccagcc tgtaatgtta cattcgcagc tgaagctaat gagtgcacca ctcttataaa 29700
atgcaccaca gaacatgaaa agctgcttat tcgccacaaa aacaaaattg gcaagtatgc 29760
tgtttatgct atttggcagc caggtgacac tacagagtat aatgttacag ttttccaggg 29820
taaaagtcat aaaactttta tgtatacttt tccattttat gaaatgtgcg acattaccat 29880
gtacatgagc aaacagtata agttgtggcc cccacaaaat tgtgtggaaa acactggcac 29940
tttctgctgc actgctatgc taattacagt gctcgctttg gtctgtaccc tactctatat 30000
taaatacaaa agcagacgca gctttattga ggaaaagaaa atgccttaat ttactaagtt 30060
acaaagctaa tgtcaccact aactgcttta ctcgctgctt gcaaaacaaa ttcaaaaagt 30120
tagcattata attagaatag gatttaaacc ccccggtcat ttcctgctca ataccattcc 30180
cctgaacaat tgactctatg tgggatatgc tccagcgcta caaccttgaa gtcaggcttc 30240
ctggatgtca gcatctgact ttggccagca cctgtcccgc ggatttgttc cagtccaact 30300
acagcgaccc accctaacag agatgaccaa cacaaccaac gcggccgccg ctaccggact 30360
tacatctacc acaaatacac cccaagtttc tgcctttgtc aataactggg ataacttggg 30420
catgtggtgg ttctccatag cgcttatgtt tgtatgcctt attattatgt ggctcatctg 30480
ctgcctaaag cgcaaacgcg cccgaccacc catctatagt cccatcattg tgctacaccc 30540
aaacaatgat ggaatccata gattggacgg actgaaacac atgttctttt ctcttacagt 30600
atgattaaat gagacatgat tcctcgagtt tttatattac tgacccttgt tgcgcttttt 30660
tgtgcgtgct ccacattggc tgcggtttct cacatcgaag tagactgcat tccagccttc 30720
acagtctatt tgctttacgg atttgtcacc ctcacgctca tctgcagcct catcactgtg 30780
gtcatcgcct ttatccagtg cattgactgg gtctgtgtgc gctttgcata tctcagacac 30840
catccccagt acagggacag gactatagct gagcttctta gaattcttta attatgaaat 30900
ttactgtgac ttttctgctg attatttgca ccctatctgc gttttgttcc ccgacctcca 30960
agcctcaaag acatatatca tgcagattca ctcgtatatg gaatattcca agttgctaca 31020
atgaaaaaag cgatctttcc gaagcctggt tatatgcaat catctctgtt atggtgttct 31080
gcagtaccat cttagcccta gctatatatc cctaccttga cattggctgg aacgcaatag 31140
atgccatgaa ccacccaact ttccccgcgc ccgctatgct tccactgcaa caagttgttg 31200
ccggcggctt tgtcccagcc aatcagcctc gcccaccttc tcccaccccc actgaaatca 31260
gctactttaa tctaacagga ggagatgact gacaccctag atctagaaat ggacggaatt 31320
attacagagc agcgcctgct agaaagacgc agggcagcgg ccgagcaaca gcgcatgaat 31380
caagagctcc aagacatggt taacttgcac cagtgcaaaa ggggtatctt ttgtctggta 31440
aagcaggcca aagtcaccta cgacagtaat accaccggac accgccttag ctacaagttg 31500
ccaaccaagc gtcagaaatt ggtggtcatg gtgggagaaa agcccattac cataactcag 31560
cactcggtag aaaccgaagg ctgcattcac tcaccttgtc aaggacctga ggatctctgc 31620
acccttatta agaccctgtg cggtctcaaa gatcttattc cctttaacta ataaaaaaaa 31680
ataataaagc atcacttact taaaatcagt tagcaaattt ctgtccagtt tattcagcag 31740
cacctccttg ccctcctccc agctctggta ttgcagcttc ctcctggctg caaactttct 31800
ccacaatcta aatggaatgt cagtttcctc ctgttcctgt ccatccgcac ccactatctt 31860
catgttgttg cagatgaagc gcgcaagacc gtctgaagat accttcaacc ccgtgtatcc 31920
atatgacacg gaaaccggtc ctccaactgt gccttttctt actcctccct ttgtatcccc 31980
caatgggttt caagagagtc cccctggggt actctctttg cgcctatccg aacctctagt 32040
tacctccaat ggcatgcttg cgctcaaaat gggcaacggc ctctctctgg acgaggccgg 32100
caaccttacc tcccaaaatg taaccactgt gagcccacct ctcaaaaaaa ccaagtcaaa 32160
cataaacctg gaaatatctg cacccctcac agttacctca gaagccctaa ctgtggctgc 32220
cgccgcacct ctaatggtcg cgggcaacac actcaccatg caatcacagg ccccgctaac 32280
cgtgcacgac tccaaactta gcattgccac ccaaggaccc ctcacagtgt cagaaggaaa 32340
gctagccctg caaacatcag gccccctcac caccaccgat agcagtaccc ttactatcac 32400
tgcctcaccc cctctaacta ctgccactgg tagcttgggc attgacttga aagagcccat 32460
ttatacacaa aatggaaaac taggactaaa gtacggggct cctttgcatg taacagacga 32520
cctaaacact ttgaccgtag caactggtcc aggtgtgact attaataata cttccttgca 32580
aactaaagtt actggagcct tgggttttga ttcacaaggc aatatgcaac ttaatgtagc 32640
aggaggacta aggattgatt ctcaaaacag acgccttata cttgatgtta gttatccgtt 32700
tgatgctcaa aaccaactaa atctaagact aggacagggc cctcttttta taaactcagc 32760
ccacaacttg gatattaact acaacaaagg cctttacttg tttacagctt caaacaattc 32820
caaaaagctt gaggttaacc taagcactgc caaggggttg atgtttgacg ctacagccat 32880
agccattaat gcaggagatg ggcttgaatt tggttcacct aatgcaccaa acacaaatcc 32940
cctcaaaaca aaaattggcc atggcctaga atttgattca aacaaggcta tggttcctaa 33000
actaggaact ggccttagtt ttgacagcac aggtgccatt acagtaggaa acaaaaataa 33060
tgataagcta actttgtgga ccacaccagc tccatctcct aactgtagac taaatgcaga 33120
gaaagatgct aaactcactt tggtcttaac aaaatgtggc agtcaaatac ttgctacagt 33180
ttcagttttg gctgttaaag gcagtttggc tccaatatct ggaacagttc aaagtgctca 33240
tcttattata agatttgacg aaaatggagt gctactaaac aattccttcc tggacccaga 33300
atattggaac tttagaaatg gagatcttac tgaaggcaca gcctatacaa acgctgttgg 33360
atttatgcct aacctatcag cttatccaaa atctcacggt aaaactgcca aaagtaacat 33420
tgtcagtcaa gtttacttaa acggagacaa aactaaacct gtaacactaa ccattacact 33480
aaacggtaca caggaaacag gagacacaac tccaagtgca tactctatgt cattttcatg 33540
ggactggtct ggccacaact acattaatga aatatttgcc acatcctctt acactttttc 33600
atacattgcc caagaataaa gaatcgtttg tgttatgttt caacgtgttt atttttcaat 33660
tgcagaaaat ttcaagtcat ttttcattca gtagtatagc cccaccacca catagcttat 33720
acagatcacc gtacctcaac tttgtataat aaagttgtaa tcaaactcac agaaccctag 33780
tattcaacct gccacctccc tcccaacaca cagagtacac agtcctttct ccccggctgg 33840
ccttaaaaag catcatatca tgggtaacag acatattctt aggtgttata ttccacacgg 33900
tttcctgtcg agccaaacgc tcatcagtga tattaataaa ctccccgggc agctcactta 33960
agttcatgtc gctgtccagc tgctgagcca caggctgctg tccaacttgc ggttgcttaa 34020
cgggcggcga aggagaagtc cacgcctaca tgggggtaga gtcataatcg tgcatcagga 34080
tagggcggtg gtgctgcagc agcgcgcgaa taaactgctg ccgccgccgc tccgtcctgc 34140
aggaatacaa catggcagtg gtctcctcag cgatgattcg caccgcccgc agcataaggc 34200
gccttgtcct ccgggcacag cagcgcaccc tgatctcact taaatcagca cagtaactgc 34260
agcacagcac cacaatattg ttcaaaatcc cacagtgcaa ggcgctgtat ccaaagctca 34320
tggcggggac cacagaaccc acgtggccat cataccacaa gcgcaggtag attaagtggc 34380
gacccctcat aaacacgctg gacataaaca ttacctcttt tggcatgttg taattcacca 34440
cctcccggta ccatataaac ctctgattaa acatggcgcc atccaccacc atcctaaacc 34500
agctggccaa aacctgcccg ccggctatac actgcaggga accgggactg gaacaatgac 34560
agtggagagc ccaggactcg taaccatgga tcatcatgct cgtcatgata tcaatgttgg 34620
cacaacacag gcacacgtgc atacacttcc tcaggattac aagctcctcc cgcgttagaa 34680
ccatatccca gggaacaacc cattcctgaa tcagcgtaaa tcccacactg cagggaagac 34740
ctcgcacgta actcacgttg tgcattgtca aagtgttaca ttcgggcagc agcggatgat 34800
cctccagtat ggtagcgcgg gtttctgtct caaaaggagg tagacgatcc ctactgtacg 34860
gagtgcgccg agacaaccga gatcgtgttg gtcgtagtgt catgccaaat ggaacgccgg 34920
acgtagtcat atttcctgaa gcaaaaccag gtgcgggcgt gacaaacaga tctgcgtctc 34980
cggtctcgcc gcttagatcg ctctgtgtag tagttgtagt atatccactc tctcaaagca 35040
tccaggcgcc ccctggcttc gggttctatg taaactcctt catgcgccgc tgccctgata 35100
acatccacca ccgcagaata agccacaccc agccaaccta cacattcgtt ctgcgagtca 35160
cacacgggag gagcgggaag agctggaaga accatgtttt tttttttatt ccaaaagatt 35220
atccaaaacc tcaaaatgaa gatctattaa gtgaacgcgc tcccctccgg tggcgtggtc 35280
aaactctaca gccaaagaac agataatggc atttgtaaga tgttgcacaa tggcttccaa 35340
aaggcaaacg gccctcacgt ccaagtggac gtaaaggcta aacccttcag ggtgaatctc 35400
ctctataaac attccagcac cttcaaccat gcccaaataa ttctcatctc gccaccttct 35460
caatatatct ctaagcaaat cccgaatatt aagtccggcc attgtaaaaa tctgctccag 35520
agcgccctcc accttcagcc tcaagcagcg aatcatgatt gcaaaaattc aggttcctca 35580
cagacctgta taagattcaa aagcggaaca ttaacaaaaa taccgcgatc ccgtaggtcc 35640
cttcgcaggg ccagctgaac ataatcgtgc aggtctgcac ggaccagcgc ggccacttcc 35700
ccgccaggaa ccatgacaaa agaacccaca ctgattatga cacgcatact cggagctatg 35760
ctaaccagcg tagccccgat gtaagcttgt tgcatgggcg gcgatataaa atgcaaggtg 35820
ctgctcaaaa aatcaggcaa agcctcgcgc aaaaaagaaa gcacatcgta gtcatgctca 35880
tgcagataaa ggcaggtaag ctccggaacc accacagaaa aagacaccat ttttctctca 35940
aacatgtctg cgggtttctg cataaacaca aaataaaata acaaaaaaac atttaaacat 36000
tagaagcctg tcttacaaca ggaaaaacaa cccttataag cataagacgg actacggcca 36060
tgccggcgtg accgtaaaaa aactggtcac cgtgattaaa aagcaccacc gacagctcct 36120
cggtcatgtc cggagtcata atgtaagact cggtaaacac atcaggttga ttcacatcgg 36180
tcagtgctaa aaagcgaccg aaatagcccg ggggaataca tacccgcagg cgtagagaca 36240
acattacagc ccccatagga ggtataacaa aattaatagg agagaaaaac acataaacac 36300
ctgaaaaacc ctcctgccta ggcaaaatag caccctcccg ctccagaaca acatacagcg 36360
cttccacagc ggcagccata acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa 36420
aacaccactc gacacggcac cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag 36480
agcgagtata tataggacta aaaaatgacg taacggttaa agtccacaaa aaacacccag 36540
aaaaccgcac gcgaacctac gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa 36600
tcgtcacttc cgttttccca cgttacgtca cttcccattt taagaaaact acaattccca 36660
acacatacaa gttactccgc cctaaaacct acgtcacccg ccccgttccc acgccccgcg 36720
ccacgtcaca aactccaccc cctcattatc atattggctt caatccaaaa taaggtatat 36780
tattgatgat g 36791
<210> 2
<211> 36791
<212> DNA
<213> 人工序列
<220>
<223> 合成腺病毒基因组E1A-P2A-YPet
<400> 2
catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60
ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120
gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180
gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240
taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300
agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360
gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420
cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480
tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540
tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600
aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660
tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720
cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780
gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840
cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900
ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960
cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020
caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080
ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140
tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200
gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260
ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320
cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380
ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440
gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500
cctgggcaac ctttggactt gagctgtaaa cgccccaggc caggaagcgg agctactaac 1560
ttcagcctgc tgaagcaggc tggtgacgtc gaggagaatc ctggcccaat ggtgagcaaa 1620
ggcgaagagc tgttcaccgg cgtggtgccc atcctggtgg agctggacgg cgacgtgaac 1680
ggccacaagt tcagcgtgag cggcgagggc gagggcgacg ccacctacgg caagctgacc 1740
ctgaagctgc tgtgcaccac cggcaagctg cccgtgccct ggcccaccct ggtgaccacc 1800
ctgggctacg gcgtgcagtg cttcgcccgg taccccgacc acatgaagca gcacgacttc 1860
ttcaagagcg ccatgcccga gggctacgtg caggagcgga ccatcttctt caaggacgac 1920
ggcaactaca agacccgggc cgaggtgaag ttcgagggcg acaccctggt gaaccggatc 1980
gagctgaagg gcatcgactt caaggaggac ggcaacatcc tgggccacaa gctggagtac 2040
aactacaaca gccacaacgt gtacatcacc gccgacaagc agaagaacgg catcaaggcc 2100
aacttcaaga tccggcacaa catcgaggac ggcggcgtgc agctggccga ccactaccag 2160
cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac 2220
cagagcgccc tgttcaagga ccccaacgag aagcgggacc acatggtgct gctggagttc 2280
ctgaccgccg ccggcatcac cgagggcatg aacgagctct ataagtaagg tgtaaacctg 2340
tgattgcgtg tgtggttaac gcctttgttt gctgaatgag ttgatgtaag tttaataaag 2400
ggtgagataa tgtttaactt gcatggcgtg ttaaatgggg cggggcttaa agggtatata 2460
atgcgccgtg ggctaatctt ggttacatct gacctcatgg aggcttggga gtgtttggaa 2520
gatttttctg ctgtgcgtaa cttgctggaa cagagctcta acagtacctc ttggttttgg 2580
aggtttctgt ggggctcatc ccaggcaaag ttagtctgca gaattaagga ggattacaag 2640
tgggaatttg aagagctttt gaaatcctgt ggtgagctgt ttgattcttt gaatctgggt 2700
caccaggcgc ttttccaaga gaaggtcatc aagactttgg atttttccac accggggcgc 2760
gctgcggctg ctgttgcttt tttgagtttt ataaaggata aatggagcga agaaacccat 2820
ctgagcgggg ggtacctgct ggattttctg gccatgcatc tgtggagagc ggttgtgaga 2880
cacaagaatc gcctgctact gttgtcttcc gtccgcccgg cgataatacc gacggaggag 2940
cagcagcagc agcaggagga agccaggcgg cggcggcagg agcagagccc atggaacccg 3000
agagccggcc tggaccctcg ggaatgaatg ttgtacaggt ggctgaactg tatccagaac 3060
tgagacgcat tttgacaatt acagaggatg ggcaggggct aaagggggta aagagggagc 3120
ggggggcttg tgaggctaca gaggaggcta ggaatctagc ttttagctta atgaccagac 3180
accgtcctga gtgtattact tttcaacaga tcaaggataa ttgcgctaat gagcttgatc 3240
tgctggcgca gaagtattcc atagagcagc tgaccactta ctggctgcag ccaggggatg 3300
attttgagga ggctattagg gtatatgcaa aggtggcact taggccagat tgcaagtaca 3360
agatcagcaa acttgtaaat atcaggaatt gttgctacat ttctgggaac ggggccgagg 3420
tggagataga tacggaggat agggtggcct ttagatgtag catgataaat atgtggccgg 3480
gggtgcttgg catggacggg gtggttatta tgaatgtaag gtttactggc cccaatttta 3540
gcggtacggt tttcctggcc aataccaacc ttatcctaca cggtgtaagc ttctatgggt 3600
ttaacaatac ctgtgtggaa gcctggaccg atgtaagggt tcggggctgt gccttttact 3660
gctgctggaa gggggtggtg tgtcgcccca aaagcagggc ttcaattaag aaatgcctct 3720
ttgaaaggtg taccttgggt atcctgtctg agggtaactc cagggtgcgc cacaatgtgg 3780
cctccgactg tggttgcttc atgctagtga aaagcgtggc tgtgattaag cataacatgg 3840
tatgtggcaa ctgcgaggac agggcctctc agatgctgac ctgctcggac ggcaactgtc 3900
acctgctgaa gaccattcac gtagccagcc actctcgcaa ggcctggcca gtgtttgagc 3960
ataacatact gacccgctgt tccttgcatt tgggtaacag gaggggggtg ttcctacctt 4020
accaatgcaa tttgagtcac actaagatat tgcttgagcc cgagagcatg tccaaggtga 4080
acctgaacgg ggtgtttgac atgaccatga agatctggaa ggtgctgagg tacgatgaga 4140
cccgcaccag gtgcagaccc tgcgagtgtg gcggtaaaca tattaggaac cagcctgtga 4200
tgctggatgt gaccgaggag ctgaggcccg atcacttggt gctggcctgc acccgcgctg 4260
agtttggctc tagcgatgaa gatacagatt gaggtactga aatgtgtggg cgtggcttaa 4320
gggtgggaaa gaatatataa ggtgggggtc ttatgtagtt ttgtatctgt tttgcagcag 4380
ccgccgccgc ccaacttttc tatacaaagt tgtaatgagc accaactcgt ttgatggaag 4440
cattgtgagc tcatatttga caacgcgcat gcccccatgg gccggggtgc gtcagaatgt 4500
gatgggctcc agcattgatg gtcgccccgt cctgcccgca aactctacta ccttgaccta 4560
cgagaccgtg tctggaacgc cgttggagac tgcagcctcc gccgccgctt cagccgctgc 4620
agccaccgcc cgcgggattg tgactgactt tgctttcctg agcccgcttg caagcagtgc 4680
agcttcccgt tcatccgccc gcgatgacaa gttgacggct cttttggcac aattggattc 4740
tttgacccgg gaacttaatg tcgtttctca gcagctgttg gatctgcgcc agcaggtttc 4800
tgccctgaag gcttcctccc ctcccaatgc ggtttaaaac ataaataaaa aaccagactc 4860
tgtttggatt tggatcaagc ataagtgtct tgctgtcttt atttaggggt tttgcgcgcg 4920
cggtaggccc gggaccagcg gtctcggtcg ttgagggtcc tgtgtatttt ttccaggacg 4980
tggtaaaggt gactctggat gttcagatac atgggcataa gcccgtctct ggggtggagg 5040
tagcaccact gcagagcttc atgctgcggg gtggtgttgt agatgatcca gtcgtagcag 5100
gagcgctggg cgtggtgcct aaaaatgtct ttcagtagca agctgattgc caggggcagg 5160
cccttggtgt aagtgtttac aaagcggtta agctgggatg ggtgcatacg tggggatatg 5220
agatgcatct tggactgtat ttttaggttg gctatgttcc cagccatatc cctccgggga 5280
ttcatgttgt gcagaaccac cagcacagtg tatccggtgc acttgggaaa tttgtcatgt 5340
agcttagaag gaaatgcgtg gaagaacttg gagacgccct tgtgacctcc aagattttcc 5400
atgcattcgt ccataatgat ggcaatgggc ccacgggcgg cggcctgggc gaagatattt 5460
ctgggatcac taacgtcata gttgtgttcc aggatgagat cgtcataggc catttttaca 5520
aagcgcgggc ggagggtgcc agactgcggt ataatggttc catccggccc aggggcgtag 5580
ttaccctcac agatttgcat ttcccacgct ttgagttcag atggggggat catgtctacc 5640
tgcggggcga tgaagaaaac ggtttccggg gtaggggaga tcagctggga agaaagcagg 5700
ttcctgagca gctgcgactt accgcagccg gtgggcccgt aaatcacacc tattaccggg 5760
tgcaactggt agttaagaga gctgcagctg ccgtcatccc tgagcagggg ggccacttcg 5820
ttaagcatgt ccctgactcg catgttttcc ctgaccaaat ccgccagaag gcgctcgccg 5880
cccagcgata gcagttcttg caaggaagca aagtttttca acggtttgag accgtccgcc 5940
gtaggcatgc ttttgagcgt ttgaccaagc agttccaggc ggtcccacag ctcggtcacc 6000
tgctctacgg catctcgatc cagcatatct cctcgtttcg cgggttgggg cggctttcgc 6060
tgtacggcag tagtcggtgc tcgtccagac gggccagggt catgtctttc cacgggcgca 6120
gggtcctcgt cagcgtagtc tgggtcacgg tgaaggggtg cgctccgggc tgcgcgctgg 6180
ccagggtgcg cttgaggctg gtcctgctgg tgctgaagcg ctgccggtct tcgccctgcg 6240
cgtcggccag gtagcatttg accatggtgt catagtccag cccctccgcg gcgtggccct 6300
tggcgcgcag cttgcccttg gaggaggcgc cgcacgaggg gcagtgcaga cttttgaggg 6360
cgtagagctt gggcgcgaga aataccgatt ccggggagta ggcatccgcg ccgcaggccc 6420
cgcagacggt ctcgcattcc acgagccagg tgagctctgg ccgttcgggg tcaaaaacca 6480
ggtttccccc atgctttttg atgcgtttct tacctctggt ttccatgagc cggtgtccac 6540
gctcggtgac gaaaaggctg tccgtgtccc cgtatacaga cttgagaggc ctgtcctcga 6600
gcggtgttcc gcggtcctcc tcgtatagaa actcggacca ctctgagaca aaggctcgcg 6660
tccaggccag cacgaaggag gctaagtggg aggggtagcg gtcgttgtcc actagggggt 6720
ccactcgctc cagggtgtga agacacatgt cgccctcttc ggcatcaagg aaggtgattg 6780
gtttgtaggt gtaggccacg tgaccgggtg ttcctgaagg ggggctataa aagggggtgg 6840
gggcgcgttc gtcctcactc tcttccgcat cgctgtctgc gagggccagc tgttggggtg 6900
agtactccct ctgaaaagcg ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg 6960
aggaggattt gatattcacc tggcccgcgg tgatgccttt gagggtggcc gcatccatct 7020
ggtcagaaaa gacaatcttt ttgttgtcaa gcttggtggc aaacgacccg tagagggcgt 7080
tggacagcaa cttggcgatg gagcgcaggg tttggttttt gtcgcgatcg gcgcgctcct 7140
tggccgcgat gtttagctgc acgtattcgc gcgcaacgca ccgccattcg ggaaagacgg 7200
tggtgcgctc gtcgggcacc aggtgcacgc gccaaccgcg gttgtgcagg gtgacaaggt 7260
caacgctggt ggctacctct ccgcgtaggc gctcgttggt ccagcagagg cggccgccct 7320
tgcgcgagca gaatggcggt agggggtcta gctgcgtctc gtccgggggg tctgcgtcca 7380
cggtaaagac cccgggcagc aggcgcgcgt cgaagtagtc tatcttgcat ccttgcaagt 7440
ctagcgcctg ctgccatgcg cgggcggcaa gcgcgcgctc gtatgggttg agtgggggac 7500
cccatggcat ggggtgggtg agcgcggagg cgtacatgcc gcaaatgtcg taaacgtaga 7560
ggggctctct gagtattcca agatatgtag ggtagcatct tccaccgcgg atgctggcgc 7620
gcacgtaatc gtatagttcg tgcgagggag cgaggaggtc gggaccgagg ttgctacggg 7680
cgggctgctc tgctcggaag actatctgcc tgaagatggc atgtgagttg gatgatatgg 7740
ttggacgctg gaagacgttg aagctggcgt ctgtgagacc taccgcgtca cgcacgaagg 7800
aggcgtagga gtcgcgcagc ttgttgacca gctcggcggt gacctgcacg tctagggcgc 7860
agtagtccag ggtttccttg atgatgtcat acttatcctg tccctttttt ttccacagct 7920
cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg 7980
cctccgaacg gtaagagcct agcatgtaga actggttgac ggcctggtag gcgcagcatc 8040
ccttttctac gggtagcgcg tatgcctgcg cggccttccg gagcgaggtg tgggtgagcg 8100
caaaggtgtc cctgaccatg actttgaggt actggtattt gaagtcagtg tcgtcgcatc 8160
cgccctgctc ccagagcaaa aagtccgtgc gctttttgga acgcggattt ggcagggcga 8220
aggtgacatc gttgaagagt atctttcccg cgcgaggcat aaagttgcgt gtgatgcgga 8280
agggtcccgg cacctcggaa cggttgttaa ttacctgggc ggcgagcacg atctcgtcaa 8340
agccgttgat gttgtggccc acaatgtaaa gttccaagaa gcgcgggatg cccttgatgg 8400
aaggcaattt tttaagttcc tcgtaggtga gctcttcagg ggagctgagc ccgtgctctg 8460
aaagggccca gtctgcaaga tgagggttgg aagcgacgaa tgagctccac aggtcacggg 8520
ccattagcat ttgcaggtgg tcgcgaaagg tcctaaactg gcgacctatg gccatttttt 8580
ctggggtgat gcagtagaag gtaagcgggt cttgttccca gcggtcccat ccaaggttcg 8640
cggctaggtc tcgcgcggca gtcactagag gctcatctcc gccgaacttc atgaccagca 8700
tgaagggcac gagctgcttc ccaaaggccc ccatccaagt ataggtctct acatcgtagg 8760
tgacaaagag acgctcggtg cgaggatgcg agccgatcgg gaagaactgg atctcccgcc 8820
accaattgga ggagtggcta ttgatgtggt gaaagtagaa gtccctgcga cgggccgaac 8880
actcgtgctg gcttttgtaa aaacgtgcgc agtactggca gcggtgcacg ggctgtacat 8940
cctgcacgag gttgacctga cgaccgcgca caaggaagca gagtgggaat ttgagcccct 9000
cgcctggcgg gtttggctgg tggtcttcta cttcggctgc ttgtccttga ccgtctggct 9060
gctcgagggg agttacggtg gatcggacca ccacgccgcg cgagcccaaa gtccagatgt 9120
ccgcgcgcgg cggtcggagc ttgatgacaa catcgcgcag atgggagctg tccatggtct 9180
ggagctcccg cggcgtcagg tcaggcggga gctcctgcag gtttacctcg catagacggg 9240
tcagggcgcg ggctagatcc aggtgatacc taatttccag gggctggttg gtggcggcgt 9300
cgatggcttg caagaggccg catccccgcg gcgcgactac ggtaccgcgc ggcgggcggt 9360
gggccgcggg ggtgtccttg gatgatgcat ctaaaagcgg tgacgcgggc gagcccccgg 9420
aggtaggggg ggctccggac ccgccgggag agggggcagg ggcacgtcgg cgccgcgcgc 9480
gggcaggagc tggtgctgcg cgcgtaggtt gctggcgaac gcgacgacgc ggcggttgat 9540
ctcctgaatc tggcgcctct gcgtgaagac gacgggcccg gtgagcttga gcctgaaaga 9600
gagttcgaca gaatcaattt cggtgtcgtt gacggcggcc tggcgcaaaa tctcctgcac 9660
gtctcctgag ttgtcttgat aggcgatctc ggccatgaac tgctcgatct cttcctcctg 9720
gagatctccg cgtccggctc gctccacggt ggcggcgagg tcgttggaaa tgcgggccat 9780
gagctgcgag aaggcgttga ggcctccctc gttccagacg cggctgtaga ccacgccccc 9840
ttcggcatcg cgggcgcgca tgaccacctg cgcgagattg agctccacgt gccgggcgaa 9900
gacggcgtag tttcgcaggc gctgaaagag gtagttgagg gtggtggcgg tgtgttctgc 9960
cacgaagaag tacataaccc agcgtcgcaa cgtggattcg ttgatatccc ccaaggcctc 10020
aaggcgctcc atggcctcgt agaagtccac ggcgaagttg aaaaactggg agttgcgcgc 10080
cgacacggtt aactcctcct ccagaagacg gatgagctcg gcgacagtgt cgcgcacctc 10140
gcgctcaaag gctacagggg cctcttcttc ttcttcaatc tcctcttcca taagggcctc 10200
cccttcttct tcttctggcg gcggtggggg aggggggaca cggcggcgac gacggcgcac 10260
cgggaggcgg tcgacaaagc gctcgatcat ctccccgcgg cgacggcgca tggtctcggt 10320
gacggcgcgg ccgttctcgc gggggcgcag ttggaagacg ccgcccgtca tgtcccggtt 10380
atgggttggc ggggggctgc catgcggcag ggatacggcg ctaacgatgc atctcaacaa 10440
ttgttgtgta ggtactccgc cgccgaggga cctgagcgag tccgcatcga ccggatcgga 10500
aaacctctcg agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc 10560
gggcggcagc gggcggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt 10620
aaagtaggcg gtcttgagac ggcggatggt cgacagaagc accatgtcct tgggtccggc 10680
ctgctgaatg cgcaggcggt cggccatgcc ccaggcttcg ttttgacatc ggcgcaggtc 10740
tttgtagtag tcttgcatga gcctttctac cggcacttct tcttctcctt cctcttgtcc 10800
tgcatctctt gcatctatcg ctgcggcggc ggcggagttt ggccgtaggt ggcgccctct 10860
tcctcccatg cgtgtgaccc cgaagcccct catcggctga agcagggcta ggtcggcgac 10920
aacgcgctcg gctaatatgg cctgctgcac ctgcgtgagg gtagactgga agtcatccat 10980
gtccacaaag cggtggtatg cgcccgtgtt gatggtgtaa gtgcagttgg ccataacgga 11040
ccagttaacg gtctggtgac ccggctgcga gagctcggtg tacctgagac gcgagtaagc 11100
cctcgagtca aatacgtagt cgttgcaagt ccgcaccagg tactggtatc ccaccaaaaa 11160
gtgcggcggc ggctggcggt agaggggcca gcgtagggtg gccggggctc cgggggcgag 11220
atcttccaac ataaggcgat gatatccgta gatgtacctg gacatccagg tgatgccggc 11280
ggcggtggtg gaggcgcgcg gaaagtcgcg gacgcggttc cagatgttgc gcagcggcaa 11340
aaagtgctcc atggtcggga cgctctggcc ggtcaggcgc gcgcaatcgt tgacgctcta 11400
gaccgtgcaa aaggagagcc tgtaagcggg cactcttccg tggtctggtg gataaattcg 11460
caagggtatc atggcggacg accggggttc gagccccgta tccggccgtc cgccgtgatc 11520
catgcggtta ccgcccgcgt gtcgaaccca ggtgtgcgac gtcagacaac gggggagtgc 11580
tccttttggc ttccttccag gcgcggcggc tgctgcgcta gcttttttgg ccactggccg 11640
cgcgcagcgt aagcggttag gctggaaagc gaaagcatta agtggctcgc tccctgtagc 11700
cggagggtta ttttccaagg gttgagtcgc gggacccccg gttcgagtct cggaccggcc 11760
ggactgcggc gaacgggggt ttgcctcccc gtcatgcaag accccgcttg caaattcctc 11820
cggaaacagg gacgagcccc ttttttgctt ttcccagatg catccggtgc tgcggcagat 11880
gcgcccccct cctcagcagc ggcaagagca agagcagcgg cagacatgca gggcaccctc 11940
ccctcctcct accgcgtcag gaggggcgac atccgcggtt gacgcggcag cagatggtga 12000
ttacgaaccc ccgcggcgcc gggcccggca ctacctggac ttggaggagg gcgagggcct 12060
ggcgcggcta ggagcgccct ctcctgagcg gtacccaagg gtgcagctga agcgtgatac 12120
gcgtgaggcg tacgtgccgc ggcagaacct gtttcgcgac cgcgagggag aggagcccga 12180
ggagatgcgg gatcgaaagt tccacgcagg gcgcgagctg cggcatggcc tgaatcgcga 12240
gcggttgctg cgcgaggagg actttgagcc cgacgcgcga accgggatta gtcccgcgcg 12300
cgcacacgtg gcggccgccg acctggtaac cgcatacgag cagacggtga accaggagat 12360
taactttcaa aaaagcttta acaaccacgt gcgtacgctt gtggcgcgcg aggaggtggc 12420
tataggactg atgcatctgt gggactttgt aagcgcgctg gagcaaaacc caaatagcaa 12480
gccgctcatg gcgcagctgt tccttatagt gcagcacagc agggacaacg aggcattcag 12540
ggatgcgctg ctaaacatag tagagcccga gggccgctgg ctgctcgatt tgataaacat 12600
cctgcagagc atagtggtgc aggagcgcag cttgagcctg gctgacaagg tggccgccat 12660
caactattcc atgcttagcc tgggcaagtt ttacgcccgc aagatatacc atacccctta 12720
cgttcccata gacaaggagg taaagatcga ggggttctac atgcgcatgg cgctgaaggt 12780
gcttaccttg agcgacgacc tgggcgttta tcgcaacgag cgcatccaca aggccgtgag 12840
cgtgagccgg cggcgcgagc tcagcgaccg cgagctgatg cacagcctgc aaagggccct 12900
ggctggcacg ggcagcggcg atagagaggc cgagtcctac tttgacgcgg gcgctgacct 12960
gcgctgggcc ccaagccgac gcgccctgga ggcagctggg gccggacctg ggctggcggt 13020
ggcacccgcg cgcgctggca acgtcggcgg cgtggaggaa tatgacgagg acgatgagta 13080
cgagccagag gacggcgagt actaagcggt gatgtttctg atcagatgat gcaagacgca 13140
acggacccgg cggtgcgggc ggcgctgcag agccagccgt ccggccttaa ctccacggac 13200
gactggcgcc aggtcatgga ccgcatcatg tcgctgactg cgcgcaatcc tgacgcgttc 13260
cggcagcagc cgcaggccaa ccggctctcc gcaattctgg aagcggtggt cccggcgcgc 13320
gcaaacccca cgcacgagaa ggtgctggcg atcgtaaacg cgctggccga aaacagggcc 13380
atccggcccg acgaggccgg cctggtctac gacgcgctgc ttcagcgcgt ggctcgttac 13440
aacagcggca acgtgcagac caacctggac cggctggtgg gggatgtgcg cgaggccgtg 13500
gcgcagcgtg agcgcgcgca gcagcagggc aacctgggct ccatggttgc actaaacgcc 13560
ttcctgagta cacagcccgc caacgtgccg cggggacagg aggactacac caactttgtg 13620
agcgcactgc ggctaatggt gactgagaca ccgcaaagtg aggtgtacca gtctgggcca 13680
gactattttt tccagaccag tagacaaggc ctgcagaccg taaacctgag ccaggctttc 13740
aaaaacttgc aggggctgtg gggggtgcgg gctcccacag gcgaccgcgc gaccgtgtct 13800
agcttgctga cgcccaactc gcgcctgttg ctgctgctaa tagcgccctt cacggacagt 13860
ggcagcgtgt cccgggacac atacctaggt cacttgctga cactgtaccg cgaggccata 13920
ggtcaggcgc atgtggacga gcatactttc caggagatta caagtgtcag ccgcgcgctg 13980
gggcaggagg acacgggcag cctggaggca accctaaact acctgctgac caaccggcgg 14040
cagaagatcc cctcgttgca cagtttaaac agcgaggagg agcgcatttt gcgctacgtg 14100
cagcagagcg tgagccttaa cctgatgcgc gacggggtaa cgcccagcgt ggcgctggac 14160
atgaccgcgc gcaacatgga accgggcatg tatgcctcaa accggccgtt tatcaaccgc 14220
ctaatggact acttgcatcg cgcggccgcc gtgaaccccg agtatttcac caatgccatc 14280
ttgaacccgc actggctacc gccccctggt ttctacaccg ggggattcga ggtgcccgag 14340
ggtaacgatg gattcctctg ggacgacata gacgacagcg tgttttcccc gcaaccgcag 14400
accctgctag agttgcaaca gcgcgagcag gcagaggcgg cgctgcgaaa ggaaagcttc 14460
cgcaggccaa gcagcttgtc cgatctaggc gctgcggccc cgcggtcaga tgctagtagc 14520
ccatttccaa gcttgatagg gtctcttacc agcactcgca ccacccgccc gcgcctgctg 14580
ggcgaggagg agtacctaaa caactcgctg ctgcagccgc agcgcgaaaa aaacctgcct 14640
ccggcatttc ccaacaacgg gatagagagc ctagtggaca agatgagtag atggaagacg 14700
tacgcgcagg agcacaggga cgtgccaggc ccgcgcccgc ccacccgtcg tcaaaggcac 14760
gaccgtcagc ggggtctggt gtgggaggac gatgactcgg cagacgacag cagcgtcctg 14820
gatttgggag ggagtggcaa cccgtttgcg caccttcgcc ccaggctggg gagaatgttt 14880
taaaaaaaaa aaagcatgat gcaaaataaa aaactcacca aggccatggc accgagcgtt 14940
ggttttcttg tattcccctt agtatgcggc gcgcggcgat gtatgaggaa ggtcctcctc 15000
cctcctacga gagtgtggtg agcgcggcgc cagtggcggc ggcgctgggt tctcccttcg 15060
atgctcccct ggacccgccg tttgtgcctc cgcggtacct gcggcctacc ggggggagaa 15120
acagcatccg ttactctgag ttggcacccc tattcgacac cacccgtgtg tacctggtgg 15180
acaacaagtc aacggatgtg gcatccctga actaccagaa cgaccacagc aactttctga 15240
ccacggtcat tcaaaacaat gactacagcc cgggggaggc aagcacacag accatcaatc 15300
ttgacgaccg gtcgcactgg ggcggcgacc tgaaaaccat cctgcatacc aacatgccaa 15360
atgtgaacga gttcatgttt accaataagt ttaaggcgcg ggtgatggtg tcgcgcttgc 15420
ctactaagga caatcaggtg gagctgaaat acgagtgggt ggagttcacg ctgcccgagg 15480
gcaactactc cgagaccatg accatagacc ttatgaacaa cgcgatcgtg gagcactact 15540
tgaaagtggg cagacagaac ggggttctgg aaagcgacat cggggtaaag tttgacaccc 15600
gcaacttcag actggggttt gaccccgtca ctggtcttgt catgcctggg gtatatacaa 15660
acgaagcctt ccatccagac atcattttgc tgccaggatg cggggtggac ttcacccaca 15720
gccgcctgag caacttgttg ggcatccgca agcggcaacc cttccaggag ggctttagga 15780
tcacctacga tgatctggag ggtggtaaca ttcccgcact gttggatgtg gacgcctacc 15840
aggcgagctt gaaagatgac accgaacagg gcgggggtgg cgcaggcggc agcaacagca 15900
gtggcagcgg cgcggaagag aactccaacg cggcagccgc ggcaatgcag ccggtggagg 15960
acatgaacga tcatgccatt cgcggcgaca cctttgccac acgggctgag gagaagcgcg 16020
ctgaggccga agcagcggcc gaagctgccg cccccgctgc gcaacccgag gtcgagaagc 16080
ctcagaagaa accggtgatc aaacccctga cagaggacag caagaaacgc agttacaacc 16140
taataagcaa tgacagcacc ttcacccagt accgcagctg gtaccttgca tacaactacg 16200
gcgaccctca gaccggaatc cgctcatgga ccctgctttg cactcctgac gtaacctgcg 16260
gctcggagca ggtctactgg tcgttgccag acatgatgca agaccccgtg accttccgct 16320
ccacgcgcca gatcagcaac tttccggtgg tgggcgccga gctgttgccc gtgcactcca 16380
agagcttcta caacgaccag gccgtctact cccaactcat ccgccagttt acctctctga 16440
cccacgtgtt caatcgcttt cccgagaacc agattttggc gcgcccgcca gcccccacca 16500
tcaccaccgt cagtgaaaac gttcctgctc tcacagatca cgggacgcta ccgctgcgca 16560
acagcatcgg aggagtccag cgagtgacca ttactgacgc cagacgccgc acctgcccct 16620
acgtttacaa ggccctgggc atagtctcgc cgcgcgtcct atcgagccgc actttttgag 16680
caagcatgtc catccttata tcgcccagca ataacacagg ctggggcctg cgcttcccaa 16740
gcaagatgtt tggcggggcc aagaagcgct ccgaccaaca cccagtgcgc gtgcgcgggc 16800
actaccgcgc gccctggggc gcgcacaaac gcggccgcac tgggcgcacc accgtcgatg 16860
acgccatcga cgcggtggtg gaggaggcgc gcaactacac gcccacgccg ccaccagtgt 16920
ccacagtgga cgcggccatt cagaccgtgg tgcgcggagc ccggcgctat gctaaaatga 16980
agagacggcg gaggcgcgta gcacgtcgcc accgccgccg acccggcact gccgcccaac 17040
gcgcggcggc ggccctgctt aaccgcgcac gtcgcaccgg ccgacgggcg gccatgcggg 17100
ccgctcgaag gctggccgcg ggtattgtca ctgtgccccc caggtccagg cgacgagcgg 17160
ccgccgcagc agccgcggcc attagtgcta tgactcaggg tcgcaggggc aacgtgtatt 17220
gggtgcgcga ctcggttagc ggcctgcgcg tgcccgtgcg cacccgcccc ccgcgcaact 17280
agattgcaag aaaaaactac ttagactcgt actgttgtat gtatccagcg gcggcggcgc 17340
gcaacgaagc tatgtccaag cgcaaaatca aagaagagat gctccaggtc atcgcgccgg 17400
agatctatgg ccccccgaag aaggaagagc aggattacaa gccccgaaag ctaaagcggg 17460
tcaaaaagaa aaagaaagat gatgatgatg aacttgacga cgaggtggaa ctgctgcacg 17520
ctaccgcgcc caggcgacgg gtacagtgga aaggtcgacg cgtaaaacgt gttttgcgac 17580
ccggcaccac cgtagtcttt acgcccggtg agcgctccac ccgcacctac aagcgcgtgt 17640
atgatgaggt gtacggcgac gaggacctgc ttgagcaggc caacgagcgc ctcggggagt 17700
ttgcctacgg aaagcggcat aaggacatgc tggcgttgcc gctggacgag ggcaacccaa 17760
cacctagcct aaagcccgta acactgcagc aggtgctgcc cgcgcttgca ccgtccgaag 17820
aaaagcgcgg cctaaagcgc gagtctggtg acttggcacc caccgtgcag ctgatggtac 17880
ccaagcgcca gcgactggaa gatgtcttgg aaaaaatgac cgtggaacct gggctggagc 17940
ccgaggtccg cgtgcggcca atcaagcagg tggcgccggg actgggcgtg cagaccgtgg 18000
acgttcagat acccactacc agtagcacca gtattgccac cgccacagag ggcatggaga 18060
cacaaacgtc cccggttgcc tcagcggtgg cggatgccgc ggtgcaggcg gtcgctgcgg 18120
ccgcgtccaa gacctctacg gaggtgcaaa cggacccgtg gatgtttcgc gtttcagccc 18180
cccggcgccc gcgcggttcg aggaagtacg gcgccgccag cgcgctactg cccgaatatg 18240
ccctacatcc ttccattgcg cctacccccg gctatcgtgg ctacacctac cgccccagaa 18300
gacgagcaac tacccgacgc cgaaccacca ctggaacccg ccgccgccgt cgccgtcgcc 18360
agcccgtgct ggccccgatt tccgtgcgca gggtggctcg cgaaggaggc aggaccctgg 18420
tgctgccaac agcgcgctac caccccagca tcgtttaaaa gccggtcttt gtggttcttg 18480
cagatatggc cctcacctgc cgcctccgtt tcccggtgcc gggattccga ggaagaatgc 18540
accgtaggag gggcatggcc ggccacggcc tgacgggcgg catgcgtcgt gcgcaccacc 18600
ggcggcggcg cgcgtcgcac cgtcgcatgc gcggcggtat cctgcccctc cttattccac 18660
tgatcgccgc ggcgattggc gccgtgcccg gaattgcatc cgtggccttg caggcgcaga 18720
gacactgatt aaaaacaagt tgcatgtgga aaaatcaaaa taaaaagtct ggactctcac 18780
gctcgcttgg tcctgtaact attttgtaga atggaagaca tcaactttgc gtctctggcc 18840
ccgcgacacg gctcgcgccc gttcatggga aactggcaag atatcggcac cagcaatatg 18900
agcggtggcg ccttcagctg gggctcgctg tggagcggca ttaaaaattt cggttccacc 18960
gttaagaact atggcagcaa ggcctggaac agcagcacag gccagatgct gagggataag 19020
ttgaaagagc aaaatttcca acaaaaggtg gtagatggcc tggcctctgg cattagcggg 19080
gtggtggacc tggccaacca ggcagtgcaa aataagatta acagtaagct tgatccccgc 19140
cctcccgtag aggagcctcc accggccgtg gagacagtgt ctccagaggg gcgtggcgaa 19200
aagcgtccgc gccccgacag ggaagaaact ctggtgacgc aaatagacga gcctccctcg 19260
tacgaggagg cactaaagca aggcctgccc accacccgtc ccatcgcgcc catggctacc 19320
ggagtgctgg gccagcacac acccgtaacg ctggacctgc ctccccccgc cgacacccag 19380
cagaaacctg tgctgccagg cccgaccgcc gttgttgtaa cccgtcctag ccgcgcgtcc 19440
ctgcgccgcg ccgccagcgg tccgcgatcg ttgcggcccg tagccagtgg caactggcaa 19500
agcacactga acagcatcgt gggtctgggg gtgcaatccc tgaagcgccg acgatgcttc 19560
tgaatagcta acgtgtcgta tgtgtgtcat gtatgcgtcc atgtcgccgc cagaggagct 19620
gctgagccgc cgcgcgcccg ctttccaaga tggctacccc ttcgatgatg ccgcagtggt 19680
cttacatgca catctcgggc caggacgcct cggagtacct gagccccggg ctggtgcagt 19740
ttgcccgcgc caccgagacg tacttcagcc tgaataacaa gtttagaaac cccacggtgg 19800
cgcctacgca cgacgtgacc acagaccggt cccagcgttt gacgctgcgg ttcatccctg 19860
tggaccgtga ggatactgcg tactcgtaca aggcgcggtt caccctagct gtgggtgata 19920
accgtgtgct ggacatggct tccacgtact ttgacatccg cggcgtgctg gacaggggcc 19980
ctacttttaa gccctactct ggcactgcct acaacgccct ggctcccaag ggtgccccaa 20040
atccttgcga atgggatgaa gctgctactg ctcttgaaat aaacctagaa gaagaggacg 20100
atgacaacga agacgaagta gacgagcaag ctgagcagca aaaaactcac gtatttgggc 20160
aggcgcctta ttctggtata aatattacaa aggagggtat tcaaataggt gtcgaaggtc 20220
aaacacctaa atatgccgat aaaacatttc aacctgaacc tcaaatagga gaatctcagt 20280
ggtacgaaac tgaaattaat catgcagctg ggagagtcct taaaaagact accccaatga 20340
aaccatgtta cggttcatat gcaaaaccca caaatgaaaa tggagggcaa ggcattcttg 20400
taaagcaaca aaatggaaag ctagaaagtc aagtggaaat gcaatttttc tcaactactg 20460
aggcgaccgc aggcaatggt gataacttga ctcctaaagt ggtattgtac agtgaagatg 20520
tagatataga aaccccagac actcatattt cttacatgcc cactattaag gaaggtaact 20580
cacgagaact aatgggccaa caatctatgc ccaacaggcc taattacatt gcttttaggg 20640
acaattttat tggtctaatg tattacaaca gcacgggtaa tatgggtgtt ctggcgggcc 20700
aagcatcgca gttgaatgct gttgtagatt tgcaagacag aaacacagag ctttcatacc 20760
agcttttgct tgattccatt ggtgatagaa ccaggtactt ttctatgtgg aatcaggctg 20820
ttgacagcta tgatccagat gttagaatta ttgaaaatca tggaactgaa gatgaacttc 20880
caaattactg ctttccactg ggaggtgtga ttaatacaga gactcttacc aaggtaaaac 20940
ctaaaacagg tcaggaaaat ggatgggaaa aagatgctac agaattttca gataaaaatg 21000
aaataagagt tggaaataat tttgccatgg aaatcaatct aaatgccaac ctgtggagaa 21060
atttcctgta ctccaacata gcgctgtatt tgcccgacaa gctaaagtac agtccttcca 21120
acgtaaaaat ttctgataac ccaaacacct acgactacat gaacaagcga gtggtggctc 21180
ccgggttagt ggactgctac attaaccttg gagcacgctg gtcccttgac tatatggaca 21240
acgtcaaccc atttaaccac caccgcaatg ctggcctgcg ctaccgctca atgttgctgg 21300
gcaatggtcg ctatgtgccc ttccacatcc aggtgcctca gaagttcttt gccattaaaa 21360
acctccttct cctgccgggc tcatacacct acgagtggaa cttcaggaag gatgttaaca 21420
tggttctgca gagctcccta ggaaatgacc taagggttga cggagccagc attaagtttg 21480
atagcatttg cctttacgcc accttcttcc ccatggccca caacaccgcc tccacgcttg 21540
aggccatgct tagaaacgac accaacgacc agtcctttaa cgactatctc tccgccgcca 21600
acatgctcta ccctataccc gccaacgcta ccaacgtgcc catatccatc ccctcccgca 21660
actgggcggc tttccgcggc tgggccttca cgcgccttaa gactaaggaa accccatcac 21720
tgggctcggg ctacgaccct tattacacct actctggctc tataccctac ctagatggaa 21780
ccttttacct caaccacacc tttaagaagg tggccattac ctttgactct tctgtcagct 21840
ggcctggcaa tgaccgcctg cttaccccca acgagtttga aattaagcgc tcagttgacg 21900
gggagggtta caacgttgcc cagtgtaaca tgaccaaaga ctggttcctg gtacaaatgc 21960
tagctaacta caacattggc taccagggct tctatatccc agagagctac aaggaccgca 22020
tgtactcctt ctttagaaac ttccagccca tgagccgtca ggtggtggat gatactaaat 22080
acaaggacta ccaacaggtg ggcatcctac accaacacaa caactctgga tttgttggct 22140
accttgcccc caccatgcgc gaaggacagg cctaccctgc taacttcccc tatccgctta 22200
taggcaagac cgcagttgac agcattaccc agaaaaagtt tctttgcgat cgcacccttt 22260
ggcgcatccc attctccagt aactttatgt ccatgggcgc actcacagac ctgggccaaa 22320
accttctcta cgccaactcc gcccacgcgc tagacatgac ttttgaggtg gatcccatgg 22380
acgagcccac ccttctttat gttttgtttg aagtctttga cgtggtccgt gtgcaccggc 22440
cgcaccgcgg cgtcatcgaa accgtgtacc tgcgcacgcc cttctcggcc ggcaacgcca 22500
caacataaag aagcaagcaa catcaacaac agctgccgcc atgggctcca gtgagcagga 22560
actgaaagcc attgtcaaag atcttggttg tgggccatat tttttgggca cctatgacaa 22620
gcgctttcca ggctttgttt ctccacacaa gctcgcctgc gccatagtca atacggccgg 22680
tcgcgagact gggggcgtac actggatggc ctttgcctgg aacccgcact caaaaacatg 22740
ctacctcttt gagccctttg gcttttctga ccagcgactc aagcaggttt accagtttga 22800
gtacgagtca ctcctgcgcc gtagcgccat tgcttcttcc cccgaccgct gtataacgct 22860
ggaaaagtcc acccaaagcg tacaggggcc caactcggcc gcctgtggac tattctgctg 22920
catgtttctc cacgcctttg ccaactggcc ccaaactccc atggatcaca accccaccat 22980
gaaccttatt accggggtac ccaactccat gctcaacagt ccccaggtac agcccaccct 23040
gcgtcgcaac caggaacagc tctacagctt cctggagcgc cactcgccct acttccgcag 23100
ccacagtgcg cagattagga gcgccacttc tttttgtcac ttgaaaaaca tgtaaaaata 23160
atgtactaga gacactttca ataaaggcaa atgcttttat ttgtacactc tcgggtgatt 23220
atttaccccc acccttgccg tctgcgccgt ttaaaaatca aaggggttct gccgcgcatc 23280
gctatgcgcc actggcaggg acacgttgcg atactggtgt ttagtgctcc acttaaactc 23340
aggcacaacc atccgcggca gctcggtgaa gttttcactc cacaggctgc gcaccatcac 23400
caacgcgttt agcaggtcgg gcgccgatat cttgaagtcg cagttggggc ctccgccctg 23460
cgcgcgcgag ttgcgataca cagggttgca gcactggaac actatcagcg ccgggtggtg 23520
cacgctggcc agcacgctct tgtcggagat cagatccgcg tccaggtcct ccgcgttgct 23580
cagggcgaac ggagtcaact ttggtagctg ccttcccaaa aagggcgcgt gcccaggctt 23640
tgagttgcac tcgcaccgta gtggcatcaa aaggtgaccg tgcccggtct gggcgttagg 23700
atacagcgcc tgcataaaag ccttgatctg cttaaaagcc acctgagcct ttgcgccttc 23760
agagaagaac atgccgcaag acttgccgga aaactgattg gccggacagg ccgcgtcgtg 23820
cacgcagcac cttgcgtcgg tgttggagat ctgcaccaca tttcggcccc accggttctt 23880
cacgatcttg gccttgctag actgctcctt cagcgcgcgc tgcccgtttt cgctcgtcac 23940
atccatttca atcacgtgct ccttatttat cataatgctt ccgtgtagac acttaagctc 24000
gccttcgatc tcagcgcagc ggtgcagcca caacgcgcag cccgtgggct cgtgatgctt 24060
gtaggtcacc tctgcaaacg actgcaggta cgcctgcagg aatcgcccca tcatcgtcac 24120
aaaggtcttg ttgctggtga aggtcagctg caacccgcgg tgctcctcgt tcagccaggt 24180
cttgcatacg gccgccagag cttccacttg gtcaggcagt agtttgaagt tcgcctttag 24240
atcgttatcc acgtggtact tgtccatcag cgcgcgcgca gcctccatgc ccttctccca 24300
cgcagacacg atcggcacac tcagcgggtt catcaccgta atttcacttt ccgcttcgct 24360
gggctcttcc tcttcctctt gcgtccgcat accacgcgcc actgggtcgt cttcattcag 24420
ccgccgcact gtgcgcttac ctcctttgcc atgcttgatt agcaccggtg ggttgctgaa 24480
acccaccatt tgtagcgcca catcttctct ttcttcctcg ctgtccacga ttacctctgg 24540
tgatggcggg cgctcgggct tgggagaagg gcgcttcttt ttcttcttgg gcgcaatggc 24600
caaatccgcc gccgaggtcg atggccgcgg gctgggtgtg cgcggcacca gcgcgtcttg 24660
tgatgagtct tcctcgtcct cggactcgat acgccgcctc atccgctttt ttgggggcgc 24720
ccggggaggc ggcggcgacg gggacgggga cgacacgtcc tccatggttg ggggacgtcg 24780
cgccgcaccg cgtccgcgct cgggggtggt ttcgcgctgc tcctcttccc gactggccat 24840
ttccttctcc tataggcaga aaaagatcat ggagtcagtc gagaagaagg acagcctaac 24900
cgccccctct gagttcgcca ccaccgcctc caccgatgcc gccaacgcgc ctaccacctt 24960
ccccgtcgag gcacccccgc ttgaggagga ggaagtgatt atcgagcagg acccaggttt 25020
tgtaagcgaa gacgacgagg accgctcagt accaacagag gataaaaagc aagaccagga 25080
caacgcagag gcaaacgagg aacaagtcgg gcggggggac gaaaggcatg gcgactacct 25140
agatgtggga gacgacgtgc tgttgaagca tctgcagcgc cagtgcgcca ttatctgcga 25200
cgcgttgcaa gagcgcagcg atgtgcccct cgccatagcg gatgtcagcc ttgcctacga 25260
acgccaccta ttctcaccgc gcgtaccccc caaacgccaa gaaaacggca catgcgagcc 25320
caacccgcgc ctcaacttct accccgtatt tgccgtgcca gaggtgcttg ccacctatca 25380
catctttttc caaaactgca agatacccct atcctgccgt gccaaccgca gccgagcgga 25440
caagcagctg gccttgcggc agggcgctgt catacctgat atcgcctcgc tcaacgaagt 25500
gccaaaaatc tttgagggtc ttggacgcga cgagaagcgc gcggcaaacg ctctgcaaca 25560
ggaaaacagc gaaaatgaaa gtcactctgg agtgttggtg gaactcgagg gtgacaacgc 25620
gcgcctagcc gtactaaaac gcagcatcga ggtcacccac tttgcctacc cggcacttaa 25680
cctacccccc aaggtcatga gcacagtcat gagtgagctg atcgtgcgcc gtgcgcagcc 25740
cctggagagg gatgcaaatt tgcaagaaca aacagaggag ggcctacccg cagttggcga 25800
cgagcagcta gcgcgctggc ttcaaacgcg cgagcctgcc gacttggagg agcgacgcaa 25860
actaatgatg gccgcagtgc tcgttaccgt ggagcttgag tgcatgcagc ggttctttgc 25920
tgacccggag atgcagcgca agctagagga aacattgcac tacacctttc gacagggcta 25980
cgtacgccag gcctgcaaga tctccaacgt ggagctctgc aacctggtct cctaccttgg 26040
aattttgcac gaaaaccgcc ttgggcaaaa cgtgcttcat tccacgctca agggcgaggc 26100
gcgccgcgac tacgtccgcg actgcgttta cttatttcta tgctacacct ggcagacggc 26160
catgggcgtt tggcagcagt gcttggagga gtgcaacctc aaggagctgc agaaactgct 26220
aaagcaaaac ttgaaggacc tatggacggc cttcaacgag cgctccgtgg ccgcgcacct 26280
ggcggacatc attttccccg aacgcctgct taaaaccctg caacagggtc tgccagactt 26340
caccagtcaa agcatgttgc agaactttag gaactttatc ctagagcgct caggaatctt 26400
gcccgccacc tgctgtgcac ttcctagcga ctttgtgccc attaagtacc gcgaatgccc 26460
tccgccgctt tggggccact gctaccttct gcagctagcc aactaccttg cctaccactc 26520
tgacataatg gaagacgtga gcggtgacgg tctactggag tgtcactgtc gctgcaacct 26580
atgcaccccg caccgctccc tggtttgcaa ttcgcagctg cttaacgaaa gtcaaattat 26640
cggtaccttt gagctgcagg gtccctcgcc tgacgaaaag tccgcggctc cggggttgaa 26700
actcactccg gggctgtgga cgtcggctta ccttcgcaaa tttgtacctg aggactacca 26760
cgcccacgag attaggttct acgaagacca atcccgcccg ccaaatgcgg agcttaccgc 26820
ctgcgtcatt acccagggcc acattcttgg ccaattgcaa gccatcaaca aagcccgcca 26880
agagtttctg ctacgaaagg gacggggggt ttacttggac ccccagtccg gcgaggagct 26940
caacccaatc cccccgccgc cgcagcccta tcagcagcag ccgcgggccc ttgcttccca 27000
ggatggcacc caaaaagaag ctgcagctgc cgccgccacc cacggacgag gaggaatact 27060
gggacagtca ggcagaggag gttttggacg aggaggagga ggacatgatg gaagactggg 27120
agagcctaga cgaggaagct tccgaggtcg aagaggtgtc agacgaaaca ccgtcaccct 27180
cggtcgcatt cccctcgccg gcgccccaga aatcggcaac cggttccagc atggctacaa 27240
cctccgctcc tcaggcgccg ccggcactgc ccgttcgccg acccaaccgt agatgggaca 27300
ccactggaac cagggccggt aagtccaagc agccgccgcc gttagcccaa gagcaacaac 27360
agcgccaagg ctaccgctca tggcgcgggc acaagaacgc catagttgct tgcttgcaag 27420
actgtggggg caacatctcc ttcgcccgcc gctttcttct ctaccatcac ggcgtggcct 27480
tcccccgtaa catcctgcat tactaccgtc atctctacag cccatactgc accggcggca 27540
gcggcagcgg cagcaacagc agcggccaca cagaagcaaa ggcgaccgga tagcaagact 27600
ctgacaaagc ccaagaaatc cacagcggcg gcagcagcag gaggaggagc gctgcgtctg 27660
gcgcccaacg aacccgtatc gacccgcgag cttagaaaca ggatttttcc cactctgtat 27720
gctatatttc aacagagcag gggccaagaa caagagctga aaataaaaaa caggtctctg 27780
cgatccctca cccgcagctg cctgtatcac aaaagcgaag atcagcttcg gcgcacgctg 27840
gaagacgcgg aggctctctt cagtaaatac tgcgcgctga ctcttaagga ctagtttcgc 27900
gccctttctc aaatttaagc gcgaaaacta cgtcatctcc agcggccaca cccggcgcca 27960
gcacctgtcg tcagcgccat ttcaactttg tatacaaaag ttgtgatgag caaggaaatt 28020
cccacgccct acatgtggag ttaccagcca caaatgggac ttgcggctgg agctgcccaa 28080
gactactcaa cccgaataaa ctacatgagc gcgggacccc acatgatatc ccgggtcaac 28140
ggaatacgcg cccaccgaaa ccgaattctc ctggaacagg cggctattac caccacacct 28200
cgtaataacc ttaatccccg tagttggccc gctgccctgg tgtaccagga aagtcccgct 28260
cccaccactg tggtacttcc cagagacgcc caggccgaag ttcagatgac taactcaggg 28320
gcgcagcttg cgggcggctt tcgtcacagg gtgcggtcgc ccgggcaggg tataactcac 28380
ctgacaatca gagggcgagg tattcagctc aacgacgagt cggtgagctc ctcgcttggt 28440
ctccgtccgg acgggacatt tcagatcggc ggcgccggcc gctcttcatt cacgcctcgt 28500
caggcaatcc taactctgca gacctcgtcc tctgagccgc gctctggagg cattggaact 28560
ctgcaattta ttgaggagtt tgtgccatcg gtctacttta accccttctc gggacctccc 28620
ggccactatc cggatcaatt tattcctaac tttgacgcgg taaaggactc ggcggacggc 28680
tacgactgaa tgttaagtgg agaggcagag caactgcgcc tgaaacacct ggtccactgt 28740
cgccgccaca agtgctttgc ccgcgactcc ggtgagtttt gctactttga attgcccgag 28800
gatcatatcg agggcccggc gcacggcgtc cggcttaccg cccagggaga gcttgcccgt 28860
agcctgattc gggagtttac ccagcgcccc ctgctagttg agcgggacag gggaccctgt 28920
gttctcactg tgatttgcaa ctgtcctaac cctggattac atcaagatct ttgttgccat 28980
ctctgtgctg agtataataa atacagaaat taaaatatac tggggctcct atcgccatcc 29040
tgtaaacgcc accgtcttca cccgcccaag caaaccaagg cgaaccttac ctggtacttt 29100
taacatctct ccctctgtga tttacaacag tttcaaccca gacggagtga gtctacgaga 29160
gaacctctcc gagctcagct actccatcag aaaaaacacc accctcctta cctgccggga 29220
acgtacgagt gcgtcaccgg ccgctgcacc acacctaccg cctgaccgta aaccagactt 29280
tttccggaca gacctcaata actctgttta ccagaacagg aggtgagctt agaaaaccct 29340
tagggtatta ggccaaaggc gcagctactg tggggtttat gaacaattca agcaactcta 29400
cgggctattc taattcaggt ttctctagaa tcggggttgg ggttattctc tgtcttgtga 29460
ttctctttat tcttatacta acgcttctct gcctaaggct cgccgcctgc tgtgtgcaca 29520
tttgcattta ttgtcagctt tttaaacgct ggggtcgcca cccaagatga ttaggtacat 29580
aatcctaggt ttactcaccc ttgcgtcagc ccacggtacc acccaaaagg tggattttaa 29640
ggagccagcc tgtaatgtta cattcgcagc tgaagctaat gagtgcacca ctcttataaa 29700
atgcaccaca gaacatgaaa agctgcttat tcgccacaaa aacaaaattg gcaagtatgc 29760
tgtttatgct atttggcagc caggtgacac tacagagtat aatgttacag ttttccaggg 29820
taaaagtcat aaaactttta tgtatacttt tccattttat gaaatgtgcg acattaccat 29880
gtacatgagc aaacagtata agttgtggcc cccacaaaat tgtgtggaaa acactggcac 29940
tttctgctgc actgctatgc taattacagt gctcgctttg gtctgtaccc tactctatat 30000
taaatacaaa agcagacgca gctttattga ggaaaagaaa atgccttaat ttactaagtt 30060
acaaagctaa tgtcaccact aactgcttta ctcgctgctt gcaaaacaaa ttcaaaaagt 30120
tagcattata attagaatag gatttaaacc ccccggtcat ttcctgctca ataccattcc 30180
cctgaacaat tgactctatg tgggatatgc tccagcgcta caaccttgaa gtcaggcttc 30240
ctggatgtca gcatctgact ttggccagca cctgtcccgc ggatttgttc cagtccaact 30300
acagcgaccc accctaacag agatgaccaa cacaaccaac gcggccgccg ctaccggact 30360
tacatctacc acaaatacac cccaagtttc tgcctttgtc aataactggg ataacttggg 30420
catgtggtgg ttctccatag cgcttatgtt tgtatgcctt attattatgt ggctcatctg 30480
ctgcctaaag cgcaaacgcg cccgaccacc catctatagt cccatcattg tgctacaccc 30540
aaacaatgat ggaatccata gattggacgg actgaaacac atgttctttt ctcttacagt 30600
atgattaaat gagacatgat tcctcgagtt tttatattac tgacccttgt tgcgcttttt 30660
tgtgcgtgct ccacattggc tgcggtttct cacatcgaag tagactgcat tccagccttc 30720
acagtctatt tgctttacgg atttgtcacc ctcacgctca tctgcagcct catcactgtg 30780
gtcatcgcct ttatccagtg cattgactgg gtctgtgtgc gctttgcata tctcagacac 30840
catccccagt acagggacag gactatagct gagcttctta gaattcttta attatgaaat 30900
ttactgtgac ttttctgctg attatttgca ccctatctgc gttttgttcc ccgacctcca 30960
agcctcaaag acatatatca tgcagattca ctcgtatatg gaatattcca agttgctaca 31020
atgaaaaaag cgatctttcc gaagcctggt tatatgcaat catctctgtt atggtgttct 31080
gcagtaccat cttagcccta gctatatatc cctaccttga cattggctgg aacgcaatag 31140
atgccatgaa ccacccaact ttccccgcgc ccgctatgct tccactgcaa caagttgttg 31200
ccggcggctt tgtcccagcc aatcagcctc gcccaccttc tcccaccccc actgaaatca 31260
gctactttaa tctaacagga ggagatgact gacaccctag atctagaaat ggacggaatt 31320
attacagagc agcgcctgct agaaagacgc agggcagcgg ccgagcaaca gcgcatgaat 31380
caagagctcc aagacatggt taacttgcac cagtgcaaaa ggggtatctt ttgtctggta 31440
aagcaggcca aagtcaccta cgacagtaat accaccggac accgccttag ctacaagttg 31500
ccaaccaagc gtcagaaatt ggtggtcatg gtgggagaaa agcccattac cataactcag 31560
cactcggtag aaaccgaagg ctgcattcac tcaccttgtc aaggacctga ggatctctgc 31620
acccttatta agaccctgtg cggtctcaaa gatcttattc cctttaacta ataaaaaaaa 31680
ataataaagc atcacttact taaaatcagt tagcaaattt ctgtccagtt tattcagcag 31740
cacctccttg ccctcctccc agctctggta ttgcagcttc ctcctggctg caaactttct 31800
ccacaatcta aatggaatgt cagtttcctc ctgttcctgt ccatccgcac ccactatctt 31860
catgttgttg cagatgaagc gcgcaagacc gtctgaagat accttcaacc ccgtgtatcc 31920
atatgacacg gaaaccggtc ctccaactgt gccttttctt actcctccct ttgtatcccc 31980
caatgggttt caagagagtc cccctggggt actctctttg cgcctatccg aacctctagt 32040
tacctccaat ggcatgcttg cgctcaaaat gggcaacggc ctctctctgg acgaggccgg 32100
caaccttacc tcccaaaatg taaccactgt gagcccacct ctcaaaaaaa ccaagtcaaa 32160
cataaacctg gaaatatctg cacccctcac agttacctca gaagccctaa ctgtggctgc 32220
cgccgcacct ctaatggtcg cgggcaacac actcaccatg caatcacagg ccccgctaac 32280
cgtgcacgac tccaaactta gcattgccac ccaaggaccc ctcacagtgt cagaaggaaa 32340
gctagccctg caaacatcag gccccctcac caccaccgat agcagtaccc ttactatcac 32400
tgcctcaccc cctctaacta ctgccactgg tagcttgggc attgacttga aagagcccat 32460
ttatacacaa aatggaaaac taggactaaa gtacggggct cctttgcatg taacagacga 32520
cctaaacact ttgaccgtag caactggtcc aggtgtgact attaataata cttccttgca 32580
aactaaagtt actggagcct tgggttttga ttcacaaggc aatatgcaac ttaatgtagc 32640
aggaggacta aggattgatt ctcaaaacag acgccttata cttgatgtta gttatccgtt 32700
tgatgctcaa aaccaactaa atctaagact aggacagggc cctcttttta taaactcagc 32760
ccacaacttg gatattaact acaacaaagg cctttacttg tttacagctt caaacaattc 32820
caaaaagctt gaggttaacc taagcactgc caaggggttg atgtttgacg ctacagccat 32880
agccattaat gcaggagatg ggcttgaatt tggttcacct aatgcaccaa acacaaatcc 32940
cctcaaaaca aaaattggcc atggcctaga atttgattca aacaaggcta tggttcctaa 33000
actaggaact ggccttagtt ttgacagcac aggtgccatt acagtaggaa acaaaaataa 33060
tgataagcta actttgtgga ccacaccagc tccatctcct aactgtagac taaatgcaga 33120
gaaagatgct aaactcactt tggtcttaac aaaatgtggc agtcaaatac ttgctacagt 33180
ttcagttttg gctgttaaag gcagtttggc tccaatatct ggaacagttc aaagtgctca 33240
tcttattata agatttgacg aaaatggagt gctactaaac aattccttcc tggacccaga 33300
atattggaac tttagaaatg gagatcttac tgaaggcaca gcctatacaa acgctgttgg 33360
atttatgcct aacctatcag cttatccaaa atctcacggt aaaactgcca aaagtaacat 33420
tgtcagtcaa gtttacttaa acggagacaa aactaaacct gtaacactaa ccattacact 33480
aaacggtaca caggaaacag gagacacaac tccaagtgca tactctatgt cattttcatg 33540
ggactggtct ggccacaact acattaatga aatatttgcc acatcctctt acactttttc 33600
atacattgcc caagaataaa gaatcgtttg tgttatgttt caacgtgttt atttttcaat 33660
tgcagaaaat ttcaagtcat ttttcattca gtagtatagc cccaccacca catagcttat 33720
acagatcacc gtacctcaac tttgtataat aaagttgtaa tcaaactcac agaaccctag 33780
tattcaacct gccacctccc tcccaacaca cagagtacac agtcctttct ccccggctgg 33840
ccttaaaaag catcatatca tgggtaacag acatattctt aggtgttata ttccacacgg 33900
tttcctgtcg agccaaacgc tcatcagtga tattaataaa ctccccgggc agctcactta 33960
agttcatgtc gctgtccagc tgctgagcca caggctgctg tccaacttgc ggttgcttaa 34020
cgggcggcga aggagaagtc cacgcctaca tgggggtaga gtcataatcg tgcatcagga 34080
tagggcggtg gtgctgcagc agcgcgcgaa taaactgctg ccgccgccgc tccgtcctgc 34140
aggaatacaa catggcagtg gtctcctcag cgatgattcg caccgcccgc agcataaggc 34200
gccttgtcct ccgggcacag cagcgcaccc tgatctcact taaatcagca cagtaactgc 34260
agcacagcac cacaatattg ttcaaaatcc cacagtgcaa ggcgctgtat ccaaagctca 34320
tggcggggac cacagaaccc acgtggccat cataccacaa gcgcaggtag attaagtggc 34380
gacccctcat aaacacgctg gacataaaca ttacctcttt tggcatgttg taattcacca 34440
cctcccggta ccatataaac ctctgattaa acatggcgcc atccaccacc atcctaaacc 34500
agctggccaa aacctgcccg ccggctatac actgcaggga accgggactg gaacaatgac 34560
agtggagagc ccaggactcg taaccatgga tcatcatgct cgtcatgata tcaatgttgg 34620
cacaacacag gcacacgtgc atacacttcc tcaggattac aagctcctcc cgcgttagaa 34680
ccatatccca gggaacaacc cattcctgaa tcagcgtaaa tcccacactg cagggaagac 34740
ctcgcacgta actcacgttg tgcattgtca aagtgttaca ttcgggcagc agcggatgat 34800
cctccagtat ggtagcgcgg gtttctgtct caaaaggagg tagacgatcc ctactgtacg 34860
gagtgcgccg agacaaccga gatcgtgttg gtcgtagtgt catgccaaat ggaacgccgg 34920
acgtagtcat atttcctgaa gcaaaaccag gtgcgggcgt gacaaacaga tctgcgtctc 34980
cggtctcgcc gcttagatcg ctctgtgtag tagttgtagt atatccactc tctcaaagca 35040
tccaggcgcc ccctggcttc gggttctatg taaactcctt catgcgccgc tgccctgata 35100
acatccacca ccgcagaata agccacaccc agccaaccta cacattcgtt ctgcgagtca 35160
cacacgggag gagcgggaag agctggaaga accatgtttt tttttttatt ccaaaagatt 35220
atccaaaacc tcaaaatgaa gatctattaa gtgaacgcgc tcccctccgg tggcgtggtc 35280
aaactctaca gccaaagaac agataatggc atttgtaaga tgttgcacaa tggcttccaa 35340
aaggcaaacg gccctcacgt ccaagtggac gtaaaggcta aacccttcag ggtgaatctc 35400
ctctataaac attccagcac cttcaaccat gcccaaataa ttctcatctc gccaccttct 35460
caatatatct ctaagcaaat cccgaatatt aagtccggcc attgtaaaaa tctgctccag 35520
agcgccctcc accttcagcc tcaagcagcg aatcatgatt gcaaaaattc aggttcctca 35580
cagacctgta taagattcaa aagcggaaca ttaacaaaaa taccgcgatc ccgtaggtcc 35640
cttcgcaggg ccagctgaac ataatcgtgc aggtctgcac ggaccagcgc ggccacttcc 35700
ccgccaggaa ccatgacaaa agaacccaca ctgattatga cacgcatact cggagctatg 35760
ctaaccagcg tagccccgat gtaagcttgt tgcatgggcg gcgatataaa atgcaaggtg 35820
ctgctcaaaa aatcaggcaa agcctcgcgc aaaaaagaaa gcacatcgta gtcatgctca 35880
tgcagataaa ggcaggtaag ctccggaacc accacagaaa aagacaccat ttttctctca 35940
aacatgtctg cgggtttctg cataaacaca aaataaaata acaaaaaaac atttaaacat 36000
tagaagcctg tcttacaaca ggaaaaacaa cccttataag cataagacgg actacggcca 36060
tgccggcgtg accgtaaaaa aactggtcac cgtgattaaa aagcaccacc gacagctcct 36120
cggtcatgtc cggagtcata atgtaagact cggtaaacac atcaggttga ttcacatcgg 36180
tcagtgctaa aaagcgaccg aaatagcccg ggggaataca tacccgcagg cgtagagaca 36240
acattacagc ccccatagga ggtataacaa aattaatagg agagaaaaac acataaacac 36300
ctgaaaaacc ctcctgccta ggcaaaatag caccctcccg ctccagaaca acatacagcg 36360
cttccacagc ggcagccata acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa 36420
aacaccactc gacacggcac cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag 36480
agcgagtata tataggacta aaaaatgacg taacggttaa agtccacaaa aaacacccag 36540
aaaaccgcac gcgaacctac gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa 36600
tcgtcacttc cgttttccca cgttacgtca cttcccattt taagaaaact acaattccca 36660
acacatacaa gttactccgc cctaaaacct acgtcacccg ccccgttccc acgccccgcg 36720
ccacgtcaca aactccaccc cctcattatc atattggctt caatccaaaa taaggtatat 36780
tattgatgat g 36791
<210> 3
<211> 36791
<212> DNA
<213> 人工序列
<220>
<223> 合成腺病毒基因组E1B-55k-P2A-YPet
<400> 3
catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60
ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120
gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180
gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240
taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300
agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360
gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420
cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480
tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540
tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600
aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660
tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720
cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780
gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840
cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900
ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960
cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020
caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080
ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140
tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200
gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260
ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320
cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380
ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440
gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500
cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560
ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620
gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680
cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740
ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800
tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860
gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920
caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980
gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040
agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100
aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160
cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220
gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280
gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340
gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400
gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460
tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520
ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580
tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640
agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700
tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760
gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820
acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880
gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940
aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000
ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060
gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120
tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180
acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240
aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300
tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360
gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420
tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480
ttggctctag cgatgaagat acagatggaa gcggagctac taacttcagc ctgctgaagc 3540
aggctggtga cgtcgaggag aatcctggcc caatggtgag caaaggcgaa gagctgttca 3600
ccggcgtggt gcccatcctg gtggagctgg acggcgacgt gaacggccac aagttcagcg 3660
tgagcggcga gggcgagggc gacgccacct acggcaagct gaccctgaag ctgctgtgca 3720
ccaccggcaa gctgcccgtg ccctggccca ccctggtgac caccctgggc tacggcgtgc 3780
agtgcttcgc ccggtacccc gaccacatga agcagcacga cttcttcaag agcgccatgc 3840
ccgagggcta cgtgcaggag cggaccatct tcttcaagga cgacggcaac tacaagaccc 3900
gggccgaggt gaagttcgag ggcgacaccc tggtgaaccg gatcgagctg aagggcatcg 3960
acttcaagga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca 4020
acgtgtacat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccggc 4080
acaacatcga ggacggcggc gtgcagctgg ccgaccacta ccagcagaac acccccatcg 4140
gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagagc gccctgttca 4200
aggaccccaa cgagaagcgg gaccacatgg tgctgctgga gttcctgacc gccgccggca 4260
tcaccgaggg catgaacgag ctctataagt gaggtactga aatgtgtggg cgtggcttaa 4320
gggtgggaaa gaatatataa ggtgggggtc ttatgtagtt ttgtatctgt tttgcagcag 4380
ccgccgccgc ccaacttttc tatacaaagt tgtaatgagc accaactcgt ttgatggaag 4440
cattgtgagc tcatatttga caacgcgcat gcccccatgg gccggggtgc gtcagaatgt 4500
gatgggctcc agcattgatg gtcgccccgt cctgcccgca aactctacta ccttgaccta 4560
cgagaccgtg tctggaacgc cgttggagac tgcagcctcc gccgccgctt cagccgctgc 4620
agccaccgcc cgcgggattg tgactgactt tgctttcctg agcccgcttg caagcagtgc 4680
agcttcccgt tcatccgccc gcgatgacaa gttgacggct cttttggcac aattggattc 4740
tttgacccgg gaacttaatg tcgtttctca gcagctgttg gatctgcgcc agcaggtttc 4800
tgccctgaag gcttcctccc ctcccaatgc ggtttaaaac ataaataaaa aaccagactc 4860
tgtttggatt tggatcaagc ataagtgtct tgctgtcttt atttaggggt tttgcgcgcg 4920
cggtaggccc gggaccagcg gtctcggtcg ttgagggtcc tgtgtatttt ttccaggacg 4980
tggtaaaggt gactctggat gttcagatac atgggcataa gcccgtctct ggggtggagg 5040
tagcaccact gcagagcttc atgctgcggg gtggtgttgt agatgatcca gtcgtagcag 5100
gagcgctggg cgtggtgcct aaaaatgtct ttcagtagca agctgattgc caggggcagg 5160
cccttggtgt aagtgtttac aaagcggtta agctgggatg ggtgcatacg tggggatatg 5220
agatgcatct tggactgtat ttttaggttg gctatgttcc cagccatatc cctccgggga 5280
ttcatgttgt gcagaaccac cagcacagtg tatccggtgc acttgggaaa tttgtcatgt 5340
agcttagaag gaaatgcgtg gaagaacttg gagacgccct tgtgacctcc aagattttcc 5400
atgcattcgt ccataatgat ggcaatgggc ccacgggcgg cggcctgggc gaagatattt 5460
ctgggatcac taacgtcata gttgtgttcc aggatgagat cgtcataggc catttttaca 5520
aagcgcgggc ggagggtgcc agactgcggt ataatggttc catccggccc aggggcgtag 5580
ttaccctcac agatttgcat ttcccacgct ttgagttcag atggggggat catgtctacc 5640
tgcggggcga tgaagaaaac ggtttccggg gtaggggaga tcagctggga agaaagcagg 5700
ttcctgagca gctgcgactt accgcagccg gtgggcccgt aaatcacacc tattaccggg 5760
tgcaactggt agttaagaga gctgcagctg ccgtcatccc tgagcagggg ggccacttcg 5820
ttaagcatgt ccctgactcg catgttttcc ctgaccaaat ccgccagaag gcgctcgccg 5880
cccagcgata gcagttcttg caaggaagca aagtttttca acggtttgag accgtccgcc 5940
gtaggcatgc ttttgagcgt ttgaccaagc agttccaggc ggtcccacag ctcggtcacc 6000
tgctctacgg catctcgatc cagcatatct cctcgtttcg cgggttgggg cggctttcgc 6060
tgtacggcag tagtcggtgc tcgtccagac gggccagggt catgtctttc cacgggcgca 6120
gggtcctcgt cagcgtagtc tgggtcacgg tgaaggggtg cgctccgggc tgcgcgctgg 6180
ccagggtgcg cttgaggctg gtcctgctgg tgctgaagcg ctgccggtct tcgccctgcg 6240
cgtcggccag gtagcatttg accatggtgt catagtccag cccctccgcg gcgtggccct 6300
tggcgcgcag cttgcccttg gaggaggcgc cgcacgaggg gcagtgcaga cttttgaggg 6360
cgtagagctt gggcgcgaga aataccgatt ccggggagta ggcatccgcg ccgcaggccc 6420
cgcagacggt ctcgcattcc acgagccagg tgagctctgg ccgttcgggg tcaaaaacca 6480
ggtttccccc atgctttttg atgcgtttct tacctctggt ttccatgagc cggtgtccac 6540
gctcggtgac gaaaaggctg tccgtgtccc cgtatacaga cttgagaggc ctgtcctcga 6600
gcggtgttcc gcggtcctcc tcgtatagaa actcggacca ctctgagaca aaggctcgcg 6660
tccaggccag cacgaaggag gctaagtggg aggggtagcg gtcgttgtcc actagggggt 6720
ccactcgctc cagggtgtga agacacatgt cgccctcttc ggcatcaagg aaggtgattg 6780
gtttgtaggt gtaggccacg tgaccgggtg ttcctgaagg ggggctataa aagggggtgg 6840
gggcgcgttc gtcctcactc tcttccgcat cgctgtctgc gagggccagc tgttggggtg 6900
agtactccct ctgaaaagcg ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg 6960
aggaggattt gatattcacc tggcccgcgg tgatgccttt gagggtggcc gcatccatct 7020
ggtcagaaaa gacaatcttt ttgttgtcaa gcttggtggc aaacgacccg tagagggcgt 7080
tggacagcaa cttggcgatg gagcgcaggg tttggttttt gtcgcgatcg gcgcgctcct 7140
tggccgcgat gtttagctgc acgtattcgc gcgcaacgca ccgccattcg ggaaagacgg 7200
tggtgcgctc gtcgggcacc aggtgcacgc gccaaccgcg gttgtgcagg gtgacaaggt 7260
caacgctggt ggctacctct ccgcgtaggc gctcgttggt ccagcagagg cggccgccct 7320
tgcgcgagca gaatggcggt agggggtcta gctgcgtctc gtccgggggg tctgcgtcca 7380
cggtaaagac cccgggcagc aggcgcgcgt cgaagtagtc tatcttgcat ccttgcaagt 7440
ctagcgcctg ctgccatgcg cgggcggcaa gcgcgcgctc gtatgggttg agtgggggac 7500
cccatggcat ggggtgggtg agcgcggagg cgtacatgcc gcaaatgtcg taaacgtaga 7560
ggggctctct gagtattcca agatatgtag ggtagcatct tccaccgcgg atgctggcgc 7620
gcacgtaatc gtatagttcg tgcgagggag cgaggaggtc gggaccgagg ttgctacggg 7680
cgggctgctc tgctcggaag actatctgcc tgaagatggc atgtgagttg gatgatatgg 7740
ttggacgctg gaagacgttg aagctggcgt ctgtgagacc taccgcgtca cgcacgaagg 7800
aggcgtagga gtcgcgcagc ttgttgacca gctcggcggt gacctgcacg tctagggcgc 7860
agtagtccag ggtttccttg atgatgtcat acttatcctg tccctttttt ttccacagct 7920
cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg 7980
cctccgaacg gtaagagcct agcatgtaga actggttgac ggcctggtag gcgcagcatc 8040
ccttttctac gggtagcgcg tatgcctgcg cggccttccg gagcgaggtg tgggtgagcg 8100
caaaggtgtc cctgaccatg actttgaggt actggtattt gaagtcagtg tcgtcgcatc 8160
cgccctgctc ccagagcaaa aagtccgtgc gctttttgga acgcggattt ggcagggcga 8220
aggtgacatc gttgaagagt atctttcccg cgcgaggcat aaagttgcgt gtgatgcgga 8280
agggtcccgg cacctcggaa cggttgttaa ttacctgggc ggcgagcacg atctcgtcaa 8340
agccgttgat gttgtggccc acaatgtaaa gttccaagaa gcgcgggatg cccttgatgg 8400
aaggcaattt tttaagttcc tcgtaggtga gctcttcagg ggagctgagc ccgtgctctg 8460
aaagggccca gtctgcaaga tgagggttgg aagcgacgaa tgagctccac aggtcacggg 8520
ccattagcat ttgcaggtgg tcgcgaaagg tcctaaactg gcgacctatg gccatttttt 8580
ctggggtgat gcagtagaag gtaagcgggt cttgttccca gcggtcccat ccaaggttcg 8640
cggctaggtc tcgcgcggca gtcactagag gctcatctcc gccgaacttc atgaccagca 8700
tgaagggcac gagctgcttc ccaaaggccc ccatccaagt ataggtctct acatcgtagg 8760
tgacaaagag acgctcggtg cgaggatgcg agccgatcgg gaagaactgg atctcccgcc 8820
accaattgga ggagtggcta ttgatgtggt gaaagtagaa gtccctgcga cgggccgaac 8880
actcgtgctg gcttttgtaa aaacgtgcgc agtactggca gcggtgcacg ggctgtacat 8940
cctgcacgag gttgacctga cgaccgcgca caaggaagca gagtgggaat ttgagcccct 9000
cgcctggcgg gtttggctgg tggtcttcta cttcggctgc ttgtccttga ccgtctggct 9060
gctcgagggg agttacggtg gatcggacca ccacgccgcg cgagcccaaa gtccagatgt 9120
ccgcgcgcgg cggtcggagc ttgatgacaa catcgcgcag atgggagctg tccatggtct 9180
ggagctcccg cggcgtcagg tcaggcggga gctcctgcag gtttacctcg catagacggg 9240
tcagggcgcg ggctagatcc aggtgatacc taatttccag gggctggttg gtggcggcgt 9300
cgatggcttg caagaggccg catccccgcg gcgcgactac ggtaccgcgc ggcgggcggt 9360
gggccgcggg ggtgtccttg gatgatgcat ctaaaagcgg tgacgcgggc gagcccccgg 9420
aggtaggggg ggctccggac ccgccgggag agggggcagg ggcacgtcgg cgccgcgcgc 9480
gggcaggagc tggtgctgcg cgcgtaggtt gctggcgaac gcgacgacgc ggcggttgat 9540
ctcctgaatc tggcgcctct gcgtgaagac gacgggcccg gtgagcttga gcctgaaaga 9600
gagttcgaca gaatcaattt cggtgtcgtt gacggcggcc tggcgcaaaa tctcctgcac 9660
gtctcctgag ttgtcttgat aggcgatctc ggccatgaac tgctcgatct cttcctcctg 9720
gagatctccg cgtccggctc gctccacggt ggcggcgagg tcgttggaaa tgcgggccat 9780
gagctgcgag aaggcgttga ggcctccctc gttccagacg cggctgtaga ccacgccccc 9840
ttcggcatcg cgggcgcgca tgaccacctg cgcgagattg agctccacgt gccgggcgaa 9900
gacggcgtag tttcgcaggc gctgaaagag gtagttgagg gtggtggcgg tgtgttctgc 9960
cacgaagaag tacataaccc agcgtcgcaa cgtggattcg ttgatatccc ccaaggcctc 10020
aaggcgctcc atggcctcgt agaagtccac ggcgaagttg aaaaactggg agttgcgcgc 10080
cgacacggtt aactcctcct ccagaagacg gatgagctcg gcgacagtgt cgcgcacctc 10140
gcgctcaaag gctacagggg cctcttcttc ttcttcaatc tcctcttcca taagggcctc 10200
cccttcttct tcttctggcg gcggtggggg aggggggaca cggcggcgac gacggcgcac 10260
cgggaggcgg tcgacaaagc gctcgatcat ctccccgcgg cgacggcgca tggtctcggt 10320
gacggcgcgg ccgttctcgc gggggcgcag ttggaagacg ccgcccgtca tgtcccggtt 10380
atgggttggc ggggggctgc catgcggcag ggatacggcg ctaacgatgc atctcaacaa 10440
ttgttgtgta ggtactccgc cgccgaggga cctgagcgag tccgcatcga ccggatcgga 10500
aaacctctcg agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc 10560
gggcggcagc gggcggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt 10620
aaagtaggcg gtcttgagac ggcggatggt cgacagaagc accatgtcct tgggtccggc 10680
ctgctgaatg cgcaggcggt cggccatgcc ccaggcttcg ttttgacatc ggcgcaggtc 10740
tttgtagtag tcttgcatga gcctttctac cggcacttct tcttctcctt cctcttgtcc 10800
tgcatctctt gcatctatcg ctgcggcggc ggcggagttt ggccgtaggt ggcgccctct 10860
tcctcccatg cgtgtgaccc cgaagcccct catcggctga agcagggcta ggtcggcgac 10920
aacgcgctcg gctaatatgg cctgctgcac ctgcgtgagg gtagactgga agtcatccat 10980
gtccacaaag cggtggtatg cgcccgtgtt gatggtgtaa gtgcagttgg ccataacgga 11040
ccagttaacg gtctggtgac ccggctgcga gagctcggtg tacctgagac gcgagtaagc 11100
cctcgagtca aatacgtagt cgttgcaagt ccgcaccagg tactggtatc ccaccaaaaa 11160
gtgcggcggc ggctggcggt agaggggcca gcgtagggtg gccggggctc cgggggcgag 11220
atcttccaac ataaggcgat gatatccgta gatgtacctg gacatccagg tgatgccggc 11280
ggcggtggtg gaggcgcgcg gaaagtcgcg gacgcggttc cagatgttgc gcagcggcaa 11340
aaagtgctcc atggtcggga cgctctggcc ggtcaggcgc gcgcaatcgt tgacgctcta 11400
gaccgtgcaa aaggagagcc tgtaagcggg cactcttccg tggtctggtg gataaattcg 11460
caagggtatc atggcggacg accggggttc gagccccgta tccggccgtc cgccgtgatc 11520
catgcggtta ccgcccgcgt gtcgaaccca ggtgtgcgac gtcagacaac gggggagtgc 11580
tccttttggc ttccttccag gcgcggcggc tgctgcgcta gcttttttgg ccactggccg 11640
cgcgcagcgt aagcggttag gctggaaagc gaaagcatta agtggctcgc tccctgtagc 11700
cggagggtta ttttccaagg gttgagtcgc gggacccccg gttcgagtct cggaccggcc 11760
ggactgcggc gaacgggggt ttgcctcccc gtcatgcaag accccgcttg caaattcctc 11820
cggaaacagg gacgagcccc ttttttgctt ttcccagatg catccggtgc tgcggcagat 11880
gcgcccccct cctcagcagc ggcaagagca agagcagcgg cagacatgca gggcaccctc 11940
ccctcctcct accgcgtcag gaggggcgac atccgcggtt gacgcggcag cagatggtga 12000
ttacgaaccc ccgcggcgcc gggcccggca ctacctggac ttggaggagg gcgagggcct 12060
ggcgcggcta ggagcgccct ctcctgagcg gtacccaagg gtgcagctga agcgtgatac 12120
gcgtgaggcg tacgtgccgc ggcagaacct gtttcgcgac cgcgagggag aggagcccga 12180
ggagatgcgg gatcgaaagt tccacgcagg gcgcgagctg cggcatggcc tgaatcgcga 12240
gcggttgctg cgcgaggagg actttgagcc cgacgcgcga accgggatta gtcccgcgcg 12300
cgcacacgtg gcggccgccg acctggtaac cgcatacgag cagacggtga accaggagat 12360
taactttcaa aaaagcttta acaaccacgt gcgtacgctt gtggcgcgcg aggaggtggc 12420
tataggactg atgcatctgt gggactttgt aagcgcgctg gagcaaaacc caaatagcaa 12480
gccgctcatg gcgcagctgt tccttatagt gcagcacagc agggacaacg aggcattcag 12540
ggatgcgctg ctaaacatag tagagcccga gggccgctgg ctgctcgatt tgataaacat 12600
cctgcagagc atagtggtgc aggagcgcag cttgagcctg gctgacaagg tggccgccat 12660
caactattcc atgcttagcc tgggcaagtt ttacgcccgc aagatatacc atacccctta 12720
cgttcccata gacaaggagg taaagatcga ggggttctac atgcgcatgg cgctgaaggt 12780
gcttaccttg agcgacgacc tgggcgttta tcgcaacgag cgcatccaca aggccgtgag 12840
cgtgagccgg cggcgcgagc tcagcgaccg cgagctgatg cacagcctgc aaagggccct 12900
ggctggcacg ggcagcggcg atagagaggc cgagtcctac tttgacgcgg gcgctgacct 12960
gcgctgggcc ccaagccgac gcgccctgga ggcagctggg gccggacctg ggctggcggt 13020
ggcacccgcg cgcgctggca acgtcggcgg cgtggaggaa tatgacgagg acgatgagta 13080
cgagccagag gacggcgagt actaagcggt gatgtttctg atcagatgat gcaagacgca 13140
acggacccgg cggtgcgggc ggcgctgcag agccagccgt ccggccttaa ctccacggac 13200
gactggcgcc aggtcatgga ccgcatcatg tcgctgactg cgcgcaatcc tgacgcgttc 13260
cggcagcagc cgcaggccaa ccggctctcc gcaattctgg aagcggtggt cccggcgcgc 13320
gcaaacccca cgcacgagaa ggtgctggcg atcgtaaacg cgctggccga aaacagggcc 13380
atccggcccg acgaggccgg cctggtctac gacgcgctgc ttcagcgcgt ggctcgttac 13440
aacagcggca acgtgcagac caacctggac cggctggtgg gggatgtgcg cgaggccgtg 13500
gcgcagcgtg agcgcgcgca gcagcagggc aacctgggct ccatggttgc actaaacgcc 13560
ttcctgagta cacagcccgc caacgtgccg cggggacagg aggactacac caactttgtg 13620
agcgcactgc ggctaatggt gactgagaca ccgcaaagtg aggtgtacca gtctgggcca 13680
gactattttt tccagaccag tagacaaggc ctgcagaccg taaacctgag ccaggctttc 13740
aaaaacttgc aggggctgtg gggggtgcgg gctcccacag gcgaccgcgc gaccgtgtct 13800
agcttgctga cgcccaactc gcgcctgttg ctgctgctaa tagcgccctt cacggacagt 13860
ggcagcgtgt cccgggacac atacctaggt cacttgctga cactgtaccg cgaggccata 13920
ggtcaggcgc atgtggacga gcatactttc caggagatta caagtgtcag ccgcgcgctg 13980
gggcaggagg acacgggcag cctggaggca accctaaact acctgctgac caaccggcgg 14040
cagaagatcc cctcgttgca cagtttaaac agcgaggagg agcgcatttt gcgctacgtg 14100
cagcagagcg tgagccttaa cctgatgcgc gacggggtaa cgcccagcgt ggcgctggac 14160
atgaccgcgc gcaacatgga accgggcatg tatgcctcaa accggccgtt tatcaaccgc 14220
ctaatggact acttgcatcg cgcggccgcc gtgaaccccg agtatttcac caatgccatc 14280
ttgaacccgc actggctacc gccccctggt ttctacaccg ggggattcga ggtgcccgag 14340
ggtaacgatg gattcctctg ggacgacata gacgacagcg tgttttcccc gcaaccgcag 14400
accctgctag agttgcaaca gcgcgagcag gcagaggcgg cgctgcgaaa ggaaagcttc 14460
cgcaggccaa gcagcttgtc cgatctaggc gctgcggccc cgcggtcaga tgctagtagc 14520
ccatttccaa gcttgatagg gtctcttacc agcactcgca ccacccgccc gcgcctgctg 14580
ggcgaggagg agtacctaaa caactcgctg ctgcagccgc agcgcgaaaa aaacctgcct 14640
ccggcatttc ccaacaacgg gatagagagc ctagtggaca agatgagtag atggaagacg 14700
tacgcgcagg agcacaggga cgtgccaggc ccgcgcccgc ccacccgtcg tcaaaggcac 14760
gaccgtcagc ggggtctggt gtgggaggac gatgactcgg cagacgacag cagcgtcctg 14820
gatttgggag ggagtggcaa cccgtttgcg caccttcgcc ccaggctggg gagaatgttt 14880
taaaaaaaaa aaagcatgat gcaaaataaa aaactcacca aggccatggc accgagcgtt 14940
ggttttcttg tattcccctt agtatgcggc gcgcggcgat gtatgaggaa ggtcctcctc 15000
cctcctacga gagtgtggtg agcgcggcgc cagtggcggc ggcgctgggt tctcccttcg 15060
atgctcccct ggacccgccg tttgtgcctc cgcggtacct gcggcctacc ggggggagaa 15120
acagcatccg ttactctgag ttggcacccc tattcgacac cacccgtgtg tacctggtgg 15180
acaacaagtc aacggatgtg gcatccctga actaccagaa cgaccacagc aactttctga 15240
ccacggtcat tcaaaacaat gactacagcc cgggggaggc aagcacacag accatcaatc 15300
ttgacgaccg gtcgcactgg ggcggcgacc tgaaaaccat cctgcatacc aacatgccaa 15360
atgtgaacga gttcatgttt accaataagt ttaaggcgcg ggtgatggtg tcgcgcttgc 15420
ctactaagga caatcaggtg gagctgaaat acgagtgggt ggagttcacg ctgcccgagg 15480
gcaactactc cgagaccatg accatagacc ttatgaacaa cgcgatcgtg gagcactact 15540
tgaaagtggg cagacagaac ggggttctgg aaagcgacat cggggtaaag tttgacaccc 15600
gcaacttcag actggggttt gaccccgtca ctggtcttgt catgcctggg gtatatacaa 15660
acgaagcctt ccatccagac atcattttgc tgccaggatg cggggtggac ttcacccaca 15720
gccgcctgag caacttgttg ggcatccgca agcggcaacc cttccaggag ggctttagga 15780
tcacctacga tgatctggag ggtggtaaca ttcccgcact gttggatgtg gacgcctacc 15840
aggcgagctt gaaagatgac accgaacagg gcgggggtgg cgcaggcggc agcaacagca 15900
gtggcagcgg cgcggaagag aactccaacg cggcagccgc ggcaatgcag ccggtggagg 15960
acatgaacga tcatgccatt cgcggcgaca cctttgccac acgggctgag gagaagcgcg 16020
ctgaggccga agcagcggcc gaagctgccg cccccgctgc gcaacccgag gtcgagaagc 16080
ctcagaagaa accggtgatc aaacccctga cagaggacag caagaaacgc agttacaacc 16140
taataagcaa tgacagcacc ttcacccagt accgcagctg gtaccttgca tacaactacg 16200
gcgaccctca gaccggaatc cgctcatgga ccctgctttg cactcctgac gtaacctgcg 16260
gctcggagca ggtctactgg tcgttgccag acatgatgca agaccccgtg accttccgct 16320
ccacgcgcca gatcagcaac tttccggtgg tgggcgccga gctgttgccc gtgcactcca 16380
agagcttcta caacgaccag gccgtctact cccaactcat ccgccagttt acctctctga 16440
cccacgtgtt caatcgcttt cccgagaacc agattttggc gcgcccgcca gcccccacca 16500
tcaccaccgt cagtgaaaac gttcctgctc tcacagatca cgggacgcta ccgctgcgca 16560
acagcatcgg aggagtccag cgagtgacca ttactgacgc cagacgccgc acctgcccct 16620
acgtttacaa ggccctgggc atagtctcgc cgcgcgtcct atcgagccgc actttttgag 16680
caagcatgtc catccttata tcgcccagca ataacacagg ctggggcctg cgcttcccaa 16740
gcaagatgtt tggcggggcc aagaagcgct ccgaccaaca cccagtgcgc gtgcgcgggc 16800
actaccgcgc gccctggggc gcgcacaaac gcggccgcac tgggcgcacc accgtcgatg 16860
acgccatcga cgcggtggtg gaggaggcgc gcaactacac gcccacgccg ccaccagtgt 16920
ccacagtgga cgcggccatt cagaccgtgg tgcgcggagc ccggcgctat gctaaaatga 16980
agagacggcg gaggcgcgta gcacgtcgcc accgccgccg acccggcact gccgcccaac 17040
gcgcggcggc ggccctgctt aaccgcgcac gtcgcaccgg ccgacgggcg gccatgcggg 17100
ccgctcgaag gctggccgcg ggtattgtca ctgtgccccc caggtccagg cgacgagcgg 17160
ccgccgcagc agccgcggcc attagtgcta tgactcaggg tcgcaggggc aacgtgtatt 17220
gggtgcgcga ctcggttagc ggcctgcgcg tgcccgtgcg cacccgcccc ccgcgcaact 17280
agattgcaag aaaaaactac ttagactcgt actgttgtat gtatccagcg gcggcggcgc 17340
gcaacgaagc tatgtccaag cgcaaaatca aagaagagat gctccaggtc atcgcgccgg 17400
agatctatgg ccccccgaag aaggaagagc aggattacaa gccccgaaag ctaaagcggg 17460
tcaaaaagaa aaagaaagat gatgatgatg aacttgacga cgaggtggaa ctgctgcacg 17520
ctaccgcgcc caggcgacgg gtacagtgga aaggtcgacg cgtaaaacgt gttttgcgac 17580
ccggcaccac cgtagtcttt acgcccggtg agcgctccac ccgcacctac aagcgcgtgt 17640
atgatgaggt gtacggcgac gaggacctgc ttgagcaggc caacgagcgc ctcggggagt 17700
ttgcctacgg aaagcggcat aaggacatgc tggcgttgcc gctggacgag ggcaacccaa 17760
cacctagcct aaagcccgta acactgcagc aggtgctgcc cgcgcttgca ccgtccgaag 17820
aaaagcgcgg cctaaagcgc gagtctggtg acttggcacc caccgtgcag ctgatggtac 17880
ccaagcgcca gcgactggaa gatgtcttgg aaaaaatgac cgtggaacct gggctggagc 17940
ccgaggtccg cgtgcggcca atcaagcagg tggcgccggg actgggcgtg cagaccgtgg 18000
acgttcagat acccactacc agtagcacca gtattgccac cgccacagag ggcatggaga 18060
cacaaacgtc cccggttgcc tcagcggtgg cggatgccgc ggtgcaggcg gtcgctgcgg 18120
ccgcgtccaa gacctctacg gaggtgcaaa cggacccgtg gatgtttcgc gtttcagccc 18180
cccggcgccc gcgcggttcg aggaagtacg gcgccgccag cgcgctactg cccgaatatg 18240
ccctacatcc ttccattgcg cctacccccg gctatcgtgg ctacacctac cgccccagaa 18300
gacgagcaac tacccgacgc cgaaccacca ctggaacccg ccgccgccgt cgccgtcgcc 18360
agcccgtgct ggccccgatt tccgtgcgca gggtggctcg cgaaggaggc aggaccctgg 18420
tgctgccaac agcgcgctac caccccagca tcgtttaaaa gccggtcttt gtggttcttg 18480
cagatatggc cctcacctgc cgcctccgtt tcccggtgcc gggattccga ggaagaatgc 18540
accgtaggag gggcatggcc ggccacggcc tgacgggcgg catgcgtcgt gcgcaccacc 18600
ggcggcggcg cgcgtcgcac cgtcgcatgc gcggcggtat cctgcccctc cttattccac 18660
tgatcgccgc ggcgattggc gccgtgcccg gaattgcatc cgtggccttg caggcgcaga 18720
gacactgatt aaaaacaagt tgcatgtgga aaaatcaaaa taaaaagtct ggactctcac 18780
gctcgcttgg tcctgtaact attttgtaga atggaagaca tcaactttgc gtctctggcc 18840
ccgcgacacg gctcgcgccc gttcatggga aactggcaag atatcggcac cagcaatatg 18900
agcggtggcg ccttcagctg gggctcgctg tggagcggca ttaaaaattt cggttccacc 18960
gttaagaact atggcagcaa ggcctggaac agcagcacag gccagatgct gagggataag 19020
ttgaaagagc aaaatttcca acaaaaggtg gtagatggcc tggcctctgg cattagcggg 19080
gtggtggacc tggccaacca ggcagtgcaa aataagatta acagtaagct tgatccccgc 19140
cctcccgtag aggagcctcc accggccgtg gagacagtgt ctccagaggg gcgtggcgaa 19200
aagcgtccgc gccccgacag ggaagaaact ctggtgacgc aaatagacga gcctccctcg 19260
tacgaggagg cactaaagca aggcctgccc accacccgtc ccatcgcgcc catggctacc 19320
ggagtgctgg gccagcacac acccgtaacg ctggacctgc ctccccccgc cgacacccag 19380
cagaaacctg tgctgccagg cccgaccgcc gttgttgtaa cccgtcctag ccgcgcgtcc 19440
ctgcgccgcg ccgccagcgg tccgcgatcg ttgcggcccg tagccagtgg caactggcaa 19500
agcacactga acagcatcgt gggtctgggg gtgcaatccc tgaagcgccg acgatgcttc 19560
tgaatagcta acgtgtcgta tgtgtgtcat gtatgcgtcc atgtcgccgc cagaggagct 19620
gctgagccgc cgcgcgcccg ctttccaaga tggctacccc ttcgatgatg ccgcagtggt 19680
cttacatgca catctcgggc caggacgcct cggagtacct gagccccggg ctggtgcagt 19740
ttgcccgcgc caccgagacg tacttcagcc tgaataacaa gtttagaaac cccacggtgg 19800
cgcctacgca cgacgtgacc acagaccggt cccagcgttt gacgctgcgg ttcatccctg 19860
tggaccgtga ggatactgcg tactcgtaca aggcgcggtt caccctagct gtgggtgata 19920
accgtgtgct ggacatggct tccacgtact ttgacatccg cggcgtgctg gacaggggcc 19980
ctacttttaa gccctactct ggcactgcct acaacgccct ggctcccaag ggtgccccaa 20040
atccttgcga atgggatgaa gctgctactg ctcttgaaat aaacctagaa gaagaggacg 20100
atgacaacga agacgaagta gacgagcaag ctgagcagca aaaaactcac gtatttgggc 20160
aggcgcctta ttctggtata aatattacaa aggagggtat tcaaataggt gtcgaaggtc 20220
aaacacctaa atatgccgat aaaacatttc aacctgaacc tcaaatagga gaatctcagt 20280
ggtacgaaac tgaaattaat catgcagctg ggagagtcct taaaaagact accccaatga 20340
aaccatgtta cggttcatat gcaaaaccca caaatgaaaa tggagggcaa ggcattcttg 20400
taaagcaaca aaatggaaag ctagaaagtc aagtggaaat gcaatttttc tcaactactg 20460
aggcgaccgc aggcaatggt gataacttga ctcctaaagt ggtattgtac agtgaagatg 20520
tagatataga aaccccagac actcatattt cttacatgcc cactattaag gaaggtaact 20580
cacgagaact aatgggccaa caatctatgc ccaacaggcc taattacatt gcttttaggg 20640
acaattttat tggtctaatg tattacaaca gcacgggtaa tatgggtgtt ctggcgggcc 20700
aagcatcgca gttgaatgct gttgtagatt tgcaagacag aaacacagag ctttcatacc 20760
agcttttgct tgattccatt ggtgatagaa ccaggtactt ttctatgtgg aatcaggctg 20820
ttgacagcta tgatccagat gttagaatta ttgaaaatca tggaactgaa gatgaacttc 20880
caaattactg ctttccactg ggaggtgtga ttaatacaga gactcttacc aaggtaaaac 20940
ctaaaacagg tcaggaaaat ggatgggaaa aagatgctac agaattttca gataaaaatg 21000
aaataagagt tggaaataat tttgccatgg aaatcaatct aaatgccaac ctgtggagaa 21060
atttcctgta ctccaacata gcgctgtatt tgcccgacaa gctaaagtac agtccttcca 21120
acgtaaaaat ttctgataac ccaaacacct acgactacat gaacaagcga gtggtggctc 21180
ccgggttagt ggactgctac attaaccttg gagcacgctg gtcccttgac tatatggaca 21240
acgtcaaccc atttaaccac caccgcaatg ctggcctgcg ctaccgctca atgttgctgg 21300
gcaatggtcg ctatgtgccc ttccacatcc aggtgcctca gaagttcttt gccattaaaa 21360
acctccttct cctgccgggc tcatacacct acgagtggaa cttcaggaag gatgttaaca 21420
tggttctgca gagctcccta ggaaatgacc taagggttga cggagccagc attaagtttg 21480
atagcatttg cctttacgcc accttcttcc ccatggccca caacaccgcc tccacgcttg 21540
aggccatgct tagaaacgac accaacgacc agtcctttaa cgactatctc tccgccgcca 21600
acatgctcta ccctataccc gccaacgcta ccaacgtgcc catatccatc ccctcccgca 21660
actgggcggc tttccgcggc tgggccttca cgcgccttaa gactaaggaa accccatcac 21720
tgggctcggg ctacgaccct tattacacct actctggctc tataccctac ctagatggaa 21780
ccttttacct caaccacacc tttaagaagg tggccattac ctttgactct tctgtcagct 21840
ggcctggcaa tgaccgcctg cttaccccca acgagtttga aattaagcgc tcagttgacg 21900
gggagggtta caacgttgcc cagtgtaaca tgaccaaaga ctggttcctg gtacaaatgc 21960
tagctaacta caacattggc taccagggct tctatatccc agagagctac aaggaccgca 22020
tgtactcctt ctttagaaac ttccagccca tgagccgtca ggtggtggat gatactaaat 22080
acaaggacta ccaacaggtg ggcatcctac accaacacaa caactctgga tttgttggct 22140
accttgcccc caccatgcgc gaaggacagg cctaccctgc taacttcccc tatccgctta 22200
taggcaagac cgcagttgac agcattaccc agaaaaagtt tctttgcgat cgcacccttt 22260
ggcgcatccc attctccagt aactttatgt ccatgggcgc actcacagac ctgggccaaa 22320
accttctcta cgccaactcc gcccacgcgc tagacatgac ttttgaggtg gatcccatgg 22380
acgagcccac ccttctttat gttttgtttg aagtctttga cgtggtccgt gtgcaccggc 22440
cgcaccgcgg cgtcatcgaa accgtgtacc tgcgcacgcc cttctcggcc ggcaacgcca 22500
caacataaag aagcaagcaa catcaacaac agctgccgcc atgggctcca gtgagcagga 22560
actgaaagcc attgtcaaag atcttggttg tgggccatat tttttgggca cctatgacaa 22620
gcgctttcca ggctttgttt ctccacacaa gctcgcctgc gccatagtca atacggccgg 22680
tcgcgagact gggggcgtac actggatggc ctttgcctgg aacccgcact caaaaacatg 22740
ctacctcttt gagccctttg gcttttctga ccagcgactc aagcaggttt accagtttga 22800
gtacgagtca ctcctgcgcc gtagcgccat tgcttcttcc cccgaccgct gtataacgct 22860
ggaaaagtcc acccaaagcg tacaggggcc caactcggcc gcctgtggac tattctgctg 22920
catgtttctc cacgcctttg ccaactggcc ccaaactccc atggatcaca accccaccat 22980
gaaccttatt accggggtac ccaactccat gctcaacagt ccccaggtac agcccaccct 23040
gcgtcgcaac caggaacagc tctacagctt cctggagcgc cactcgccct acttccgcag 23100
ccacagtgcg cagattagga gcgccacttc tttttgtcac ttgaaaaaca tgtaaaaata 23160
atgtactaga gacactttca ataaaggcaa atgcttttat ttgtacactc tcgggtgatt 23220
atttaccccc acccttgccg tctgcgccgt ttaaaaatca aaggggttct gccgcgcatc 23280
gctatgcgcc actggcaggg acacgttgcg atactggtgt ttagtgctcc acttaaactc 23340
aggcacaacc atccgcggca gctcggtgaa gttttcactc cacaggctgc gcaccatcac 23400
caacgcgttt agcaggtcgg gcgccgatat cttgaagtcg cagttggggc ctccgccctg 23460
cgcgcgcgag ttgcgataca cagggttgca gcactggaac actatcagcg ccgggtggtg 23520
cacgctggcc agcacgctct tgtcggagat cagatccgcg tccaggtcct ccgcgttgct 23580
cagggcgaac ggagtcaact ttggtagctg ccttcccaaa aagggcgcgt gcccaggctt 23640
tgagttgcac tcgcaccgta gtggcatcaa aaggtgaccg tgcccggtct gggcgttagg 23700
atacagcgcc tgcataaaag ccttgatctg cttaaaagcc acctgagcct ttgcgccttc 23760
agagaagaac atgccgcaag acttgccgga aaactgattg gccggacagg ccgcgtcgtg 23820
cacgcagcac cttgcgtcgg tgttggagat ctgcaccaca tttcggcccc accggttctt 23880
cacgatcttg gccttgctag actgctcctt cagcgcgcgc tgcccgtttt cgctcgtcac 23940
atccatttca atcacgtgct ccttatttat cataatgctt ccgtgtagac acttaagctc 24000
gccttcgatc tcagcgcagc ggtgcagcca caacgcgcag cccgtgggct cgtgatgctt 24060
gtaggtcacc tctgcaaacg actgcaggta cgcctgcagg aatcgcccca tcatcgtcac 24120
aaaggtcttg ttgctggtga aggtcagctg caacccgcgg tgctcctcgt tcagccaggt 24180
cttgcatacg gccgccagag cttccacttg gtcaggcagt agtttgaagt tcgcctttag 24240
atcgttatcc acgtggtact tgtccatcag cgcgcgcgca gcctccatgc ccttctccca 24300
cgcagacacg atcggcacac tcagcgggtt catcaccgta atttcacttt ccgcttcgct 24360
gggctcttcc tcttcctctt gcgtccgcat accacgcgcc actgggtcgt cttcattcag 24420
ccgccgcact gtgcgcttac ctcctttgcc atgcttgatt agcaccggtg ggttgctgaa 24480
acccaccatt tgtagcgcca catcttctct ttcttcctcg ctgtccacga ttacctctgg 24540
tgatggcggg cgctcgggct tgggagaagg gcgcttcttt ttcttcttgg gcgcaatggc 24600
caaatccgcc gccgaggtcg atggccgcgg gctgggtgtg cgcggcacca gcgcgtcttg 24660
tgatgagtct tcctcgtcct cggactcgat acgccgcctc atccgctttt ttgggggcgc 24720
ccggggaggc ggcggcgacg gggacgggga cgacacgtcc tccatggttg ggggacgtcg 24780
cgccgcaccg cgtccgcgct cgggggtggt ttcgcgctgc tcctcttccc gactggccat 24840
ttccttctcc tataggcaga aaaagatcat ggagtcagtc gagaagaagg acagcctaac 24900
cgccccctct gagttcgcca ccaccgcctc caccgatgcc gccaacgcgc ctaccacctt 24960
ccccgtcgag gcacccccgc ttgaggagga ggaagtgatt atcgagcagg acccaggttt 25020
tgtaagcgaa gacgacgagg accgctcagt accaacagag gataaaaagc aagaccagga 25080
caacgcagag gcaaacgagg aacaagtcgg gcggggggac gaaaggcatg gcgactacct 25140
agatgtggga gacgacgtgc tgttgaagca tctgcagcgc cagtgcgcca ttatctgcga 25200
cgcgttgcaa gagcgcagcg atgtgcccct cgccatagcg gatgtcagcc ttgcctacga 25260
acgccaccta ttctcaccgc gcgtaccccc caaacgccaa gaaaacggca catgcgagcc 25320
caacccgcgc ctcaacttct accccgtatt tgccgtgcca gaggtgcttg ccacctatca 25380
catctttttc caaaactgca agatacccct atcctgccgt gccaaccgca gccgagcgga 25440
caagcagctg gccttgcggc agggcgctgt catacctgat atcgcctcgc tcaacgaagt 25500
gccaaaaatc tttgagggtc ttggacgcga cgagaagcgc gcggcaaacg ctctgcaaca 25560
ggaaaacagc gaaaatgaaa gtcactctgg agtgttggtg gaactcgagg gtgacaacgc 25620
gcgcctagcc gtactaaaac gcagcatcga ggtcacccac tttgcctacc cggcacttaa 25680
cctacccccc aaggtcatga gcacagtcat gagtgagctg atcgtgcgcc gtgcgcagcc 25740
cctggagagg gatgcaaatt tgcaagaaca aacagaggag ggcctacccg cagttggcga 25800
cgagcagcta gcgcgctggc ttcaaacgcg cgagcctgcc gacttggagg agcgacgcaa 25860
actaatgatg gccgcagtgc tcgttaccgt ggagcttgag tgcatgcagc ggttctttgc 25920
tgacccggag atgcagcgca agctagagga aacattgcac tacacctttc gacagggcta 25980
cgtacgccag gcctgcaaga tctccaacgt ggagctctgc aacctggtct cctaccttgg 26040
aattttgcac gaaaaccgcc ttgggcaaaa cgtgcttcat tccacgctca agggcgaggc 26100
gcgccgcgac tacgtccgcg actgcgttta cttatttcta tgctacacct ggcagacggc 26160
catgggcgtt tggcagcagt gcttggagga gtgcaacctc aaggagctgc agaaactgct 26220
aaagcaaaac ttgaaggacc tatggacggc cttcaacgag cgctccgtgg ccgcgcacct 26280
ggcggacatc attttccccg aacgcctgct taaaaccctg caacagggtc tgccagactt 26340
caccagtcaa agcatgttgc agaactttag gaactttatc ctagagcgct caggaatctt 26400
gcccgccacc tgctgtgcac ttcctagcga ctttgtgccc attaagtacc gcgaatgccc 26460
tccgccgctt tggggccact gctaccttct gcagctagcc aactaccttg cctaccactc 26520
tgacataatg gaagacgtga gcggtgacgg tctactggag tgtcactgtc gctgcaacct 26580
atgcaccccg caccgctccc tggtttgcaa ttcgcagctg cttaacgaaa gtcaaattat 26640
cggtaccttt gagctgcagg gtccctcgcc tgacgaaaag tccgcggctc cggggttgaa 26700
actcactccg gggctgtgga cgtcggctta ccttcgcaaa tttgtacctg aggactacca 26760
cgcccacgag attaggttct acgaagacca atcccgcccg ccaaatgcgg agcttaccgc 26820
ctgcgtcatt acccagggcc acattcttgg ccaattgcaa gccatcaaca aagcccgcca 26880
agagtttctg ctacgaaagg gacggggggt ttacttggac ccccagtccg gcgaggagct 26940
caacccaatc cccccgccgc cgcagcccta tcagcagcag ccgcgggccc ttgcttccca 27000
ggatggcacc caaaaagaag ctgcagctgc cgccgccacc cacggacgag gaggaatact 27060
gggacagtca ggcagaggag gttttggacg aggaggagga ggacatgatg gaagactggg 27120
agagcctaga cgaggaagct tccgaggtcg aagaggtgtc agacgaaaca ccgtcaccct 27180
cggtcgcatt cccctcgccg gcgccccaga aatcggcaac cggttccagc atggctacaa 27240
cctccgctcc tcaggcgccg ccggcactgc ccgttcgccg acccaaccgt agatgggaca 27300
ccactggaac cagggccggt aagtccaagc agccgccgcc gttagcccaa gagcaacaac 27360
agcgccaagg ctaccgctca tggcgcgggc acaagaacgc catagttgct tgcttgcaag 27420
actgtggggg caacatctcc ttcgcccgcc gctttcttct ctaccatcac ggcgtggcct 27480
tcccccgtaa catcctgcat tactaccgtc atctctacag cccatactgc accggcggca 27540
gcggcagcgg cagcaacagc agcggccaca cagaagcaaa ggcgaccgga tagcaagact 27600
ctgacaaagc ccaagaaatc cacagcggcg gcagcagcag gaggaggagc gctgcgtctg 27660
gcgcccaacg aacccgtatc gacccgcgag cttagaaaca ggatttttcc cactctgtat 27720
gctatatttc aacagagcag gggccaagaa caagagctga aaataaaaaa caggtctctg 27780
cgatccctca cccgcagctg cctgtatcac aaaagcgaag atcagcttcg gcgcacgctg 27840
gaagacgcgg aggctctctt cagtaaatac tgcgcgctga ctcttaagga ctagtttcgc 27900
gccctttctc aaatttaagc gcgaaaacta cgtcatctcc agcggccaca cccggcgcca 27960
gcacctgtcg tcagcgccat ttcaactttg tatacaaaag ttgtgatgag caaggaaatt 28020
cccacgccct acatgtggag ttaccagcca caaatgggac ttgcggctgg agctgcccaa 28080
gactactcaa cccgaataaa ctacatgagc gcgggacccc acatgatatc ccgggtcaac 28140
ggaatacgcg cccaccgaaa ccgaattctc ctggaacagg cggctattac caccacacct 28200
cgtaataacc ttaatccccg tagttggccc gctgccctgg tgtaccagga aagtcccgct 28260
cccaccactg tggtacttcc cagagacgcc caggccgaag ttcagatgac taactcaggg 28320
gcgcagcttg cgggcggctt tcgtcacagg gtgcggtcgc ccgggcaggg tataactcac 28380
ctgacaatca gagggcgagg tattcagctc aacgacgagt cggtgagctc ctcgcttggt 28440
ctccgtccgg acgggacatt tcagatcggc ggcgccggcc gctcttcatt cacgcctcgt 28500
caggcaatcc taactctgca gacctcgtcc tctgagccgc gctctggagg cattggaact 28560
ctgcaattta ttgaggagtt tgtgccatcg gtctacttta accccttctc gggacctccc 28620
ggccactatc cggatcaatt tattcctaac tttgacgcgg taaaggactc ggcggacggc 28680
tacgactgaa tgttaagtgg agaggcagag caactgcgcc tgaaacacct ggtccactgt 28740
cgccgccaca agtgctttgc ccgcgactcc ggtgagtttt gctactttga attgcccgag 28800
gatcatatcg agggcccggc gcacggcgtc cggcttaccg cccagggaga gcttgcccgt 28860
agcctgattc gggagtttac ccagcgcccc ctgctagttg agcgggacag gggaccctgt 28920
gttctcactg tgatttgcaa ctgtcctaac cctggattac atcaagatct ttgttgccat 28980
ctctgtgctg agtataataa atacagaaat taaaatatac tggggctcct atcgccatcc 29040
tgtaaacgcc accgtcttca cccgcccaag caaaccaagg cgaaccttac ctggtacttt 29100
taacatctct ccctctgtga tttacaacag tttcaaccca gacggagtga gtctacgaga 29160
gaacctctcc gagctcagct actccatcag aaaaaacacc accctcctta cctgccggga 29220
acgtacgagt gcgtcaccgg ccgctgcacc acacctaccg cctgaccgta aaccagactt 29280
tttccggaca gacctcaata actctgttta ccagaacagg aggtgagctt agaaaaccct 29340
tagggtatta ggccaaaggc gcagctactg tggggtttat gaacaattca agcaactcta 29400
cgggctattc taattcaggt ttctctagaa tcggggttgg ggttattctc tgtcttgtga 29460
ttctctttat tcttatacta acgcttctct gcctaaggct cgccgcctgc tgtgtgcaca 29520
tttgcattta ttgtcagctt tttaaacgct ggggtcgcca cccaagatga ttaggtacat 29580
aatcctaggt ttactcaccc ttgcgtcagc ccacggtacc acccaaaagg tggattttaa 29640
ggagccagcc tgtaatgtta cattcgcagc tgaagctaat gagtgcacca ctcttataaa 29700
atgcaccaca gaacatgaaa agctgcttat tcgccacaaa aacaaaattg gcaagtatgc 29760
tgtttatgct atttggcagc caggtgacac tacagagtat aatgttacag ttttccaggg 29820
taaaagtcat aaaactttta tgtatacttt tccattttat gaaatgtgcg acattaccat 29880
gtacatgagc aaacagtata agttgtggcc cccacaaaat tgtgtggaaa acactggcac 29940
tttctgctgc actgctatgc taattacagt gctcgctttg gtctgtaccc tactctatat 30000
taaatacaaa agcagacgca gctttattga ggaaaagaaa atgccttaat ttactaagtt 30060
acaaagctaa tgtcaccact aactgcttta ctcgctgctt gcaaaacaaa ttcaaaaagt 30120
tagcattata attagaatag gatttaaacc ccccggtcat ttcctgctca ataccattcc 30180
cctgaacaat tgactctatg tgggatatgc tccagcgcta caaccttgaa gtcaggcttc 30240
ctggatgtca gcatctgact ttggccagca cctgtcccgc ggatttgttc cagtccaact 30300
acagcgaccc accctaacag agatgaccaa cacaaccaac gcggccgccg ctaccggact 30360
tacatctacc acaaatacac cccaagtttc tgcctttgtc aataactggg ataacttggg 30420
catgtggtgg ttctccatag cgcttatgtt tgtatgcctt attattatgt ggctcatctg 30480
ctgcctaaag cgcaaacgcg cccgaccacc catctatagt cccatcattg tgctacaccc 30540
aaacaatgat ggaatccata gattggacgg actgaaacac atgttctttt ctcttacagt 30600
atgattaaat gagacatgat tcctcgagtt tttatattac tgacccttgt tgcgcttttt 30660
tgtgcgtgct ccacattggc tgcggtttct cacatcgaag tagactgcat tccagccttc 30720
acagtctatt tgctttacgg atttgtcacc ctcacgctca tctgcagcct catcactgtg 30780
gtcatcgcct ttatccagtg cattgactgg gtctgtgtgc gctttgcata tctcagacac 30840
catccccagt acagggacag gactatagct gagcttctta gaattcttta attatgaaat 30900
ttactgtgac ttttctgctg attatttgca ccctatctgc gttttgttcc ccgacctcca 30960
agcctcaaag acatatatca tgcagattca ctcgtatatg gaatattcca agttgctaca 31020
atgaaaaaag cgatctttcc gaagcctggt tatatgcaat catctctgtt atggtgttct 31080
gcagtaccat cttagcccta gctatatatc cctaccttga cattggctgg aacgcaatag 31140
atgccatgaa ccacccaact ttccccgcgc ccgctatgct tccactgcaa caagttgttg 31200
ccggcggctt tgtcccagcc aatcagcctc gcccaccttc tcccaccccc actgaaatca 31260
gctactttaa tctaacagga ggagatgact gacaccctag atctagaaat ggacggaatt 31320
attacagagc agcgcctgct agaaagacgc agggcagcgg ccgagcaaca gcgcatgaat 31380
caagagctcc aagacatggt taacttgcac cagtgcaaaa ggggtatctt ttgtctggta 31440
aagcaggcca aagtcaccta cgacagtaat accaccggac accgccttag ctacaagttg 31500
ccaaccaagc gtcagaaatt ggtggtcatg gtgggagaaa agcccattac cataactcag 31560
cactcggtag aaaccgaagg ctgcattcac tcaccttgtc aaggacctga ggatctctgc 31620
acccttatta agaccctgtg cggtctcaaa gatcttattc cctttaacta ataaaaaaaa 31680
ataataaagc atcacttact taaaatcagt tagcaaattt ctgtccagtt tattcagcag 31740
cacctccttg ccctcctccc agctctggta ttgcagcttc ctcctggctg caaactttct 31800
ccacaatcta aatggaatgt cagtttcctc ctgttcctgt ccatccgcac ccactatctt 31860
catgttgttg cagatgaagc gcgcaagacc gtctgaagat accttcaacc ccgtgtatcc 31920
atatgacacg gaaaccggtc ctccaactgt gccttttctt actcctccct ttgtatcccc 31980
caatgggttt caagagagtc cccctggggt actctctttg cgcctatccg aacctctagt 32040
tacctccaat ggcatgcttg cgctcaaaat gggcaacggc ctctctctgg acgaggccgg 32100
caaccttacc tcccaaaatg taaccactgt gagcccacct ctcaaaaaaa ccaagtcaaa 32160
cataaacctg gaaatatctg cacccctcac agttacctca gaagccctaa ctgtggctgc 32220
cgccgcacct ctaatggtcg cgggcaacac actcaccatg caatcacagg ccccgctaac 32280
cgtgcacgac tccaaactta gcattgccac ccaaggaccc ctcacagtgt cagaaggaaa 32340
gctagccctg caaacatcag gccccctcac caccaccgat agcagtaccc ttactatcac 32400
tgcctcaccc cctctaacta ctgccactgg tagcttgggc attgacttga aagagcccat 32460
ttatacacaa aatggaaaac taggactaaa gtacggggct cctttgcatg taacagacga 32520
cctaaacact ttgaccgtag caactggtcc aggtgtgact attaataata cttccttgca 32580
aactaaagtt actggagcct tgggttttga ttcacaaggc aatatgcaac ttaatgtagc 32640
aggaggacta aggattgatt ctcaaaacag acgccttata cttgatgtta gttatccgtt 32700
tgatgctcaa aaccaactaa atctaagact aggacagggc cctcttttta taaactcagc 32760
ccacaacttg gatattaact acaacaaagg cctttacttg tttacagctt caaacaattc 32820
caaaaagctt gaggttaacc taagcactgc caaggggttg atgtttgacg ctacagccat 32880
agccattaat gcaggagatg ggcttgaatt tggttcacct aatgcaccaa acacaaatcc 32940
cctcaaaaca aaaattggcc atggcctaga atttgattca aacaaggcta tggttcctaa 33000
actaggaact ggccttagtt ttgacagcac aggtgccatt acagtaggaa acaaaaataa 33060
tgataagcta actttgtgga ccacaccagc tccatctcct aactgtagac taaatgcaga 33120
gaaagatgct aaactcactt tggtcttaac aaaatgtggc agtcaaatac ttgctacagt 33180
ttcagttttg gctgttaaag gcagtttggc tccaatatct ggaacagttc aaagtgctca 33240
tcttattata agatttgacg aaaatggagt gctactaaac aattccttcc tggacccaga 33300
atattggaac tttagaaatg gagatcttac tgaaggcaca gcctatacaa acgctgttgg 33360
atttatgcct aacctatcag cttatccaaa atctcacggt aaaactgcca aaagtaacat 33420
tgtcagtcaa gtttacttaa acggagacaa aactaaacct gtaacactaa ccattacact 33480
aaacggtaca caggaaacag gagacacaac tccaagtgca tactctatgt cattttcatg 33540
ggactggtct ggccacaact acattaatga aatatttgcc acatcctctt acactttttc 33600
atacattgcc caagaataaa gaatcgtttg tgttatgttt caacgtgttt atttttcaat 33660
tgcagaaaat ttcaagtcat ttttcattca gtagtatagc cccaccacca catagcttat 33720
acagatcacc gtacctcaac tttgtataat aaagttgtaa tcaaactcac agaaccctag 33780
tattcaacct gccacctccc tcccaacaca cagagtacac agtcctttct ccccggctgg 33840
ccttaaaaag catcatatca tgggtaacag acatattctt aggtgttata ttccacacgg 33900
tttcctgtcg agccaaacgc tcatcagtga tattaataaa ctccccgggc agctcactta 33960
agttcatgtc gctgtccagc tgctgagcca caggctgctg tccaacttgc ggttgcttaa 34020
cgggcggcga aggagaagtc cacgcctaca tgggggtaga gtcataatcg tgcatcagga 34080
tagggcggtg gtgctgcagc agcgcgcgaa taaactgctg ccgccgccgc tccgtcctgc 34140
aggaatacaa catggcagtg gtctcctcag cgatgattcg caccgcccgc agcataaggc 34200
gccttgtcct ccgggcacag cagcgcaccc tgatctcact taaatcagca cagtaactgc 34260
agcacagcac cacaatattg ttcaaaatcc cacagtgcaa ggcgctgtat ccaaagctca 34320
tggcggggac cacagaaccc acgtggccat cataccacaa gcgcaggtag attaagtggc 34380
gacccctcat aaacacgctg gacataaaca ttacctcttt tggcatgttg taattcacca 34440
cctcccggta ccatataaac ctctgattaa acatggcgcc atccaccacc atcctaaacc 34500
agctggccaa aacctgcccg ccggctatac actgcaggga accgggactg gaacaatgac 34560
agtggagagc ccaggactcg taaccatgga tcatcatgct cgtcatgata tcaatgttgg 34620
cacaacacag gcacacgtgc atacacttcc tcaggattac aagctcctcc cgcgttagaa 34680
ccatatccca gggaacaacc cattcctgaa tcagcgtaaa tcccacactg cagggaagac 34740
ctcgcacgta actcacgttg tgcattgtca aagtgttaca ttcgggcagc agcggatgat 34800
cctccagtat ggtagcgcgg gtttctgtct caaaaggagg tagacgatcc ctactgtacg 34860
gagtgcgccg agacaaccga gatcgtgttg gtcgtagtgt catgccaaat ggaacgccgg 34920
acgtagtcat atttcctgaa gcaaaaccag gtgcgggcgt gacaaacaga tctgcgtctc 34980
cggtctcgcc gcttagatcg ctctgtgtag tagttgtagt atatccactc tctcaaagca 35040
tccaggcgcc ccctggcttc gggttctatg taaactcctt catgcgccgc tgccctgata 35100
acatccacca ccgcagaata agccacaccc agccaaccta cacattcgtt ctgcgagtca 35160
cacacgggag gagcgggaag agctggaaga accatgtttt tttttttatt ccaaaagatt 35220
atccaaaacc tcaaaatgaa gatctattaa gtgaacgcgc tcccctccgg tggcgtggtc 35280
aaactctaca gccaaagaac agataatggc atttgtaaga tgttgcacaa tggcttccaa 35340
aaggcaaacg gccctcacgt ccaagtggac gtaaaggcta aacccttcag ggtgaatctc 35400
ctctataaac attccagcac cttcaaccat gcccaaataa ttctcatctc gccaccttct 35460
caatatatct ctaagcaaat cccgaatatt aagtccggcc attgtaaaaa tctgctccag 35520
agcgccctcc accttcagcc tcaagcagcg aatcatgatt gcaaaaattc aggttcctca 35580
cagacctgta taagattcaa aagcggaaca ttaacaaaaa taccgcgatc ccgtaggtcc 35640
cttcgcaggg ccagctgaac ataatcgtgc aggtctgcac ggaccagcgc ggccacttcc 35700
ccgccaggaa ccatgacaaa agaacccaca ctgattatga cacgcatact cggagctatg 35760
ctaaccagcg tagccccgat gtaagcttgt tgcatgggcg gcgatataaa atgcaaggtg 35820
ctgctcaaaa aatcaggcaa agcctcgcgc aaaaaagaaa gcacatcgta gtcatgctca 35880
tgcagataaa ggcaggtaag ctccggaacc accacagaaa aagacaccat ttttctctca 35940
aacatgtctg cgggtttctg cataaacaca aaataaaata acaaaaaaac atttaaacat 36000
tagaagcctg tcttacaaca ggaaaaacaa cccttataag cataagacgg actacggcca 36060
tgccggcgtg accgtaaaaa aactggtcac cgtgattaaa aagcaccacc gacagctcct 36120
cggtcatgtc cggagtcata atgtaagact cggtaaacac atcaggttga ttcacatcgg 36180
tcagtgctaa aaagcgaccg aaatagcccg ggggaataca tacccgcagg cgtagagaca 36240
acattacagc ccccatagga ggtataacaa aattaatagg agagaaaaac acataaacac 36300
ctgaaaaacc ctcctgccta ggcaaaatag caccctcccg ctccagaaca acatacagcg 36360
cttccacagc ggcagccata acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa 36420
aacaccactc gacacggcac cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag 36480
agcgagtata tataggacta aaaaatgacg taacggttaa agtccacaaa aaacacccag 36540
aaaaccgcac gcgaacctac gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa 36600
tcgtcacttc cgttttccca cgttacgtca cttcccattt taagaaaact acaattccca 36660
acacatacaa gttactccgc cctaaaacct acgtcacccg ccccgttccc acgccccgcg 36720
ccacgtcaca aactccaccc cctcattatc atattggctt caatccaaaa taaggtatat 36780
tattgatgat g 36791
<210> 4
<211> 36782
<212> DNA
<213> 人工序列
<220>
<223> 合成腺病毒基因组E1B-55k-P2A-mCherry
<400> 4
catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60
ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120
gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180
gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240
taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300
agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360
gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420
cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480
tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540
tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600
aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660
tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720
cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780
gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840
cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900
ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960
cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020
caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080
ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140
tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200
gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260
ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320
cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380
ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440
gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500
cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560
ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620
gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680
cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740
ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800
tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860
gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920
caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980
gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040
agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100
aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160
cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220
gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280
gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340
gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400
gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460
tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520
ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580
tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640
agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700
tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760
gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820
acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880
gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940
aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000
ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060
gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120
tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180
acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240
aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300
tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360
gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420
tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480
ttggctctag cgatgaagat acagatggaa gcggagctac taacttcagc ctgctgaagc 3540
aggctggtga cgtcgaggag aatcctggcc caatggtgag caagggcgag gaggataaca 3600
tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc 3660
acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca 3720
agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt 3780
tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc 3840
tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg 3900
tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc 3960
gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg 4020
cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc 4080
tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga 4140
agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca 4200
acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg 4260
gcatggacga gctgtacaag tgaggtactg aaatgtgtgg gcgtggctta agggtgggaa 4320
agaatatata aggtgggggt cttatgtagt tttgtatctg ttttgcagca gccgccgccg 4380
cccaactttt ctatacaaag ttgtaatgag caccaactcg tttgatggaa gcattgtgag 4440
ctcatatttg acaacgcgca tgcccccatg ggccggggtg cgtcagaatg tgatgggctc 4500
cagcattgat ggtcgccccg tcctgcccgc aaactctact accttgacct acgagaccgt 4560
gtctggaacg ccgttggaga ctgcagcctc cgccgccgct tcagccgctg cagccaccgc 4620
ccgcgggatt gtgactgact ttgctttcct gagcccgctt gcaagcagtg cagcttcccg 4680
ttcatccgcc cgcgatgaca agttgacggc tcttttggca caattggatt ctttgacccg 4740
ggaacttaat gtcgtttctc agcagctgtt ggatctgcgc cagcaggttt ctgccctgaa 4800
ggcttcctcc cctcccaatg cggtttaaaa cataaataaa aaaccagact ctgtttggat 4860
ttggatcaag cataagtgtc ttgctgtctt tatttagggg ttttgcgcgc gcggtaggcc 4920
cgggaccagc ggtctcggtc gttgagggtc ctgtgtattt tttccaggac gtggtaaagg 4980
tgactctgga tgttcagata catgggcata agcccgtctc tggggtggag gtagcaccac 5040
tgcagagctt catgctgcgg ggtggtgttg tagatgatcc agtcgtagca ggagcgctgg 5100
gcgtggtgcc taaaaatgtc tttcagtagc aagctgattg ccaggggcag gcccttggtg 5160
taagtgttta caaagcggtt aagctgggat gggtgcatac gtggggatat gagatgcatc 5220
ttggactgta tttttaggtt ggctatgttc ccagccatat ccctccgggg attcatgttg 5280
tgcagaacca ccagcacagt gtatccggtg cacttgggaa atttgtcatg tagcttagaa 5340
ggaaatgcgt ggaagaactt ggagacgccc ttgtgacctc caagattttc catgcattcg 5400
tccataatga tggcaatggg cccacgggcg gcggcctggg cgaagatatt tctgggatca 5460
ctaacgtcat agttgtgttc caggatgaga tcgtcatagg ccatttttac aaagcgcggg 5520
cggagggtgc cagactgcgg tataatggtt ccatccggcc caggggcgta gttaccctca 5580
cagatttgca tttcccacgc tttgagttca gatgggggga tcatgtctac ctgcggggcg 5640
atgaagaaaa cggtttccgg ggtaggggag atcagctggg aagaaagcag gttcctgagc 5700
agctgcgact taccgcagcc ggtgggcccg taaatcacac ctattaccgg gtgcaactgg 5760
tagttaagag agctgcagct gccgtcatcc ctgagcaggg gggccacttc gttaagcatg 5820
tccctgactc gcatgttttc cctgaccaaa tccgccagaa ggcgctcgcc gcccagcgat 5880
agcagttctt gcaaggaagc aaagtttttc aacggtttga gaccgtccgc cgtaggcatg 5940
cttttgagcg tttgaccaag cagttccagg cggtcccaca gctcggtcac ctgctctacg 6000
gcatctcgat ccagcatatc tcctcgtttc gcgggttggg gcggctttcg ctgtacggca 6060
gtagtcggtg ctcgtccaga cgggccaggg tcatgtcttt ccacgggcgc agggtcctcg 6120
tcagcgtagt ctgggtcacg gtgaaggggt gcgctccggg ctgcgcgctg gccagggtgc 6180
gcttgaggct ggtcctgctg gtgctgaagc gctgccggtc ttcgccctgc gcgtcggcca 6240
ggtagcattt gaccatggtg tcatagtcca gcccctccgc ggcgtggccc ttggcgcgca 6300
gcttgccctt ggaggaggcg ccgcacgagg ggcagtgcag acttttgagg gcgtagagct 6360
tgggcgcgag aaataccgat tccggggagt aggcatccgc gccgcaggcc ccgcagacgg 6420
tctcgcattc cacgagccag gtgagctctg gccgttcggg gtcaaaaacc aggtttcccc 6480
catgcttttt gatgcgtttc ttacctctgg tttccatgag ccggtgtcca cgctcggtga 6540
cgaaaaggct gtccgtgtcc ccgtatacag acttgagagg cctgtcctcg agcggtgttc 6600
cgcggtcctc ctcgtataga aactcggacc actctgagac aaaggctcgc gtccaggcca 6660
gcacgaagga ggctaagtgg gaggggtagc ggtcgttgtc cactaggggg tccactcgct 6720
ccagggtgtg aagacacatg tcgccctctt cggcatcaag gaaggtgatt ggtttgtagg 6780
tgtaggccac gtgaccgggt gttcctgaag gggggctata aaagggggtg ggggcgcgtt 6840
cgtcctcact ctcttccgca tcgctgtctg cgagggccag ctgttggggt gagtactccc 6900
tctgaaaagc gggcatgact tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt 6960
tgatattcac ctggcccgcg gtgatgcctt tgagggtggc cgcatccatc tggtcagaaa 7020
agacaatctt tttgttgtca agcttggtgg caaacgaccc gtagagggcg ttggacagca 7080
acttggcgat ggagcgcagg gtttggtttt tgtcgcgatc ggcgcgctcc ttggccgcga 7140
tgtttagctg cacgtattcg cgcgcaacgc accgccattc gggaaagacg gtggtgcgct 7200
cgtcgggcac caggtgcacg cgccaaccgc ggttgtgcag ggtgacaagg tcaacgctgg 7260
tggctacctc tccgcgtagg cgctcgttgg tccagcagag gcggccgccc ttgcgcgagc 7320
agaatggcgg tagggggtct agctgcgtct cgtccggggg gtctgcgtcc acggtaaaga 7380
ccccgggcag caggcgcgcg tcgaagtagt ctatcttgca tccttgcaag tctagcgcct 7440
gctgccatgc gcgggcggca agcgcgcgct cgtatgggtt gagtggggga ccccatggca 7500
tggggtgggt gagcgcggag gcgtacatgc cgcaaatgtc gtaaacgtag aggggctctc 7560
tgagtattcc aagatatgta gggtagcatc ttccaccgcg gatgctggcg cgcacgtaat 7620
cgtatagttc gtgcgaggga gcgaggaggt cgggaccgag gttgctacgg gcgggctgct 7680
ctgctcggaa gactatctgc ctgaagatgg catgtgagtt ggatgatatg gttggacgct 7740
ggaagacgtt gaagctggcg tctgtgagac ctaccgcgtc acgcacgaag gaggcgtagg 7800
agtcgcgcag cttgttgacc agctcggcgg tgacctgcac gtctagggcg cagtagtcca 7860
gggtttcctt gatgatgtca tacttatcct gtcccttttt tttccacagc tcgcggttga 7920
ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac 7980
ggtaagagcc tagcatgtag aactggttga cggcctggta ggcgcagcat cccttttcta 8040
cgggtagcgc gtatgcctgc gcggccttcc ggagcgaggt gtgggtgagc gcaaaggtgt 8100
ccctgaccat gactttgagg tactggtatt tgaagtcagt gtcgtcgcat ccgccctgct 8160
cccagagcaa aaagtccgtg cgctttttgg aacgcggatt tggcagggcg aaggtgacat 8220
cgttgaagag tatctttccc gcgcgaggca taaagttgcg tgtgatgcgg aagggtcccg 8280
gcacctcgga acggttgtta attacctggg cggcgagcac gatctcgtca aagccgttga 8340
tgttgtggcc cacaatgtaa agttccaaga agcgcgggat gcccttgatg gaaggcaatt 8400
ttttaagttc ctcgtaggtg agctcttcag gggagctgag cccgtgctct gaaagggccc 8460
agtctgcaag atgagggttg gaagcgacga atgagctcca caggtcacgg gccattagca 8520
tttgcaggtg gtcgcgaaag gtcctaaact ggcgacctat ggccattttt tctggggtga 8580
tgcagtagaa ggtaagcggg tcttgttccc agcggtccca tccaaggttc gcggctaggt 8640
ctcgcgcggc agtcactaga ggctcatctc cgccgaactt catgaccagc atgaagggca 8700
cgagctgctt cccaaaggcc cccatccaag tataggtctc tacatcgtag gtgacaaaga 8760
gacgctcggt gcgaggatgc gagccgatcg ggaagaactg gatctcccgc caccaattgg 8820
aggagtggct attgatgtgg tgaaagtaga agtccctgcg acgggccgaa cactcgtgct 8880
ggcttttgta aaaacgtgcg cagtactggc agcggtgcac gggctgtaca tcctgcacga 8940
ggttgacctg acgaccgcgc acaaggaagc agagtgggaa tttgagcccc tcgcctggcg 9000
ggtttggctg gtggtcttct acttcggctg cttgtccttg accgtctggc tgctcgaggg 9060
gagttacggt ggatcggacc accacgccgc gcgagcccaa agtccagatg tccgcgcgcg 9120
gcggtcggag cttgatgaca acatcgcgca gatgggagct gtccatggtc tggagctccc 9180
gcggcgtcag gtcaggcggg agctcctgca ggtttacctc gcatagacgg gtcagggcgc 9240
gggctagatc caggtgatac ctaatttcca ggggctggtt ggtggcggcg tcgatggctt 9300
gcaagaggcc gcatccccgc ggcgcgacta cggtaccgcg cggcgggcgg tgggccgcgg 9360
gggtgtcctt ggatgatgca tctaaaagcg gtgacgcggg cgagcccccg gaggtagggg 9420
gggctccgga cccgccggga gagggggcag gggcacgtcg gcgccgcgcg cgggcaggag 9480
ctggtgctgc gcgcgtaggt tgctggcgaa cgcgacgacg cggcggttga tctcctgaat 9540
ctggcgcctc tgcgtgaaga cgacgggccc ggtgagcttg agcctgaaag agagttcgac 9600
agaatcaatt tcggtgtcgt tgacggcggc ctggcgcaaa atctcctgca cgtctcctga 9660
gttgtcttga taggcgatct cggccatgaa ctgctcgatc tcttcctcct ggagatctcc 9720
gcgtccggct cgctccacgg tggcggcgag gtcgttggaa atgcgggcca tgagctgcga 9780
gaaggcgttg aggcctccct cgttccagac gcggctgtag accacgcccc cttcggcatc 9840
gcgggcgcgc atgaccacct gcgcgagatt gagctccacg tgccgggcga agacggcgta 9900
gtttcgcagg cgctgaaaga ggtagttgag ggtggtggcg gtgtgttctg ccacgaagaa 9960
gtacataacc cagcgtcgca acgtggattc gttgatatcc cccaaggcct caaggcgctc 10020
catggcctcg tagaagtcca cggcgaagtt gaaaaactgg gagttgcgcg ccgacacggt 10080
taactcctcc tccagaagac ggatgagctc ggcgacagtg tcgcgcacct cgcgctcaaa 10140
ggctacaggg gcctcttctt cttcttcaat ctcctcttcc ataagggcct ccccttcttc 10200
ttcttctggc ggcggtgggg gaggggggac acggcggcga cgacggcgca ccgggaggcg 10260
gtcgacaaag cgctcgatca tctccccgcg gcgacggcgc atggtctcgg tgacggcgcg 10320
gccgttctcg cgggggcgca gttggaagac gccgcccgtc atgtcccggt tatgggttgg 10380
cggggggctg ccatgcggca gggatacggc gctaacgatg catctcaaca attgttgtgt 10440
aggtactccg ccgccgaggg acctgagcga gtccgcatcg accggatcgg aaaacctctc 10500
gagaaaggcg tctaaccagt cacagtcgca aggtaggctg agcaccgtgg cgggcggcag 10560
cgggcggcgg tcggggttgt ttctggcgga ggtgctgctg atgatgtaat taaagtaggc 10620
ggtcttgaga cggcggatgg tcgacagaag caccatgtcc ttgggtccgg cctgctgaat 10680
gcgcaggcgg tcggccatgc cccaggcttc gttttgacat cggcgcaggt ctttgtagta 10740
gtcttgcatg agcctttcta ccggcacttc ttcttctcct tcctcttgtc ctgcatctct 10800
tgcatctatc gctgcggcgg cggcggagtt tggccgtagg tggcgccctc ttcctcccat 10860
gcgtgtgacc ccgaagcccc tcatcggctg aagcagggct aggtcggcga caacgcgctc 10920
ggctaatatg gcctgctgca cctgcgtgag ggtagactgg aagtcatcca tgtccacaaa 10980
gcggtggtat gcgcccgtgt tgatggtgta agtgcagttg gccataacgg accagttaac 11040
ggtctggtga cccggctgcg agagctcggt gtacctgaga cgcgagtaag ccctcgagtc 11100
aaatacgtag tcgttgcaag tccgcaccag gtactggtat cccaccaaaa agtgcggcgg 11160
cggctggcgg tagaggggcc agcgtagggt ggccggggct ccgggggcga gatcttccaa 11220
cataaggcga tgatatccgt agatgtacct ggacatccag gtgatgccgg cggcggtggt 11280
ggaggcgcgc ggaaagtcgc ggacgcggtt ccagatgttg cgcagcggca aaaagtgctc 11340
catggtcggg acgctctggc cggtcaggcg cgcgcaatcg ttgacgctct agaccgtgca 11400
aaaggagagc ctgtaagcgg gcactcttcc gtggtctggt ggataaattc gcaagggtat 11460
catggcggac gaccggggtt cgagccccgt atccggccgt ccgccgtgat ccatgcggtt 11520
accgcccgcg tgtcgaaccc aggtgtgcga cgtcagacaa cgggggagtg ctccttttgg 11580
cttccttcca ggcgcggcgg ctgctgcgct agcttttttg gccactggcc gcgcgcagcg 11640
taagcggtta ggctggaaag cgaaagcatt aagtggctcg ctccctgtag ccggagggtt 11700
attttccaag ggttgagtcg cgggaccccc ggttcgagtc tcggaccggc cggactgcgg 11760
cgaacggggg tttgcctccc cgtcatgcaa gaccccgctt gcaaattcct ccggaaacag 11820
ggacgagccc cttttttgct tttcccagat gcatccggtg ctgcggcaga tgcgcccccc 11880
tcctcagcag cggcaagagc aagagcagcg gcagacatgc agggcaccct cccctcctcc 11940
taccgcgtca ggaggggcga catccgcggt tgacgcggca gcagatggtg attacgaacc 12000
cccgcggcgc cgggcccggc actacctgga cttggaggag ggcgagggcc tggcgcggct 12060
aggagcgccc tctcctgagc ggtacccaag ggtgcagctg aagcgtgata cgcgtgaggc 12120
gtacgtgccg cggcagaacc tgtttcgcga ccgcgaggga gaggagcccg aggagatgcg 12180
ggatcgaaag ttccacgcag ggcgcgagct gcggcatggc ctgaatcgcg agcggttgct 12240
gcgcgaggag gactttgagc ccgacgcgcg aaccgggatt agtcccgcgc gcgcacacgt 12300
ggcggccgcc gacctggtaa ccgcatacga gcagacggtg aaccaggaga ttaactttca 12360
aaaaagcttt aacaaccacg tgcgtacgct tgtggcgcgc gaggaggtgg ctataggact 12420
gatgcatctg tgggactttg taagcgcgct ggagcaaaac ccaaatagca agccgctcat 12480
ggcgcagctg ttccttatag tgcagcacag cagggacaac gaggcattca gggatgcgct 12540
gctaaacata gtagagcccg agggccgctg gctgctcgat ttgataaaca tcctgcagag 12600
catagtggtg caggagcgca gcttgagcct ggctgacaag gtggccgcca tcaactattc 12660
catgcttagc ctgggcaagt tttacgcccg caagatatac catacccctt acgttcccat 12720
agacaaggag gtaaagatcg aggggttcta catgcgcatg gcgctgaagg tgcttacctt 12780
gagcgacgac ctgggcgttt atcgcaacga gcgcatccac aaggccgtga gcgtgagccg 12840
gcggcgcgag ctcagcgacc gcgagctgat gcacagcctg caaagggccc tggctggcac 12900
gggcagcggc gatagagagg ccgagtccta ctttgacgcg ggcgctgacc tgcgctgggc 12960
cccaagccga cgcgccctgg aggcagctgg ggccggacct gggctggcgg tggcacccgc 13020
gcgcgctggc aacgtcggcg gcgtggagga atatgacgag gacgatgagt acgagccaga 13080
ggacggcgag tactaagcgg tgatgtttct gatcagatga tgcaagacgc aacggacccg 13140
gcggtgcggg cggcgctgca gagccagccg tccggcctta actccacgga cgactggcgc 13200
caggtcatgg accgcatcat gtcgctgact gcgcgcaatc ctgacgcgtt ccggcagcag 13260
ccgcaggcca accggctctc cgcaattctg gaagcggtgg tcccggcgcg cgcaaacccc 13320
acgcacgaga aggtgctggc gatcgtaaac gcgctggccg aaaacagggc catccggccc 13380
gacgaggccg gcctggtcta cgacgcgctg cttcagcgcg tggctcgtta caacagcggc 13440
aacgtgcaga ccaacctgga ccggctggtg ggggatgtgc gcgaggccgt ggcgcagcgt 13500
gagcgcgcgc agcagcaggg caacctgggc tccatggttg cactaaacgc cttcctgagt 13560
acacagcccg ccaacgtgcc gcggggacag gaggactaca ccaactttgt gagcgcactg 13620
cggctaatgg tgactgagac accgcaaagt gaggtgtacc agtctgggcc agactatttt 13680
ttccagacca gtagacaagg cctgcagacc gtaaacctga gccaggcttt caaaaacttg 13740
caggggctgt ggggggtgcg ggctcccaca ggcgaccgcg cgaccgtgtc tagcttgctg 13800
acgcccaact cgcgcctgtt gctgctgcta atagcgccct tcacggacag tggcagcgtg 13860
tcccgggaca catacctagg tcacttgctg acactgtacc gcgaggccat aggtcaggcg 13920
catgtggacg agcatacttt ccaggagatt acaagtgtca gccgcgcgct ggggcaggag 13980
gacacgggca gcctggaggc aaccctaaac tacctgctga ccaaccggcg gcagaagatc 14040
ccctcgttgc acagtttaaa cagcgaggag gagcgcattt tgcgctacgt gcagcagagc 14100
gtgagcctta acctgatgcg cgacggggta acgcccagcg tggcgctgga catgaccgcg 14160
cgcaacatgg aaccgggcat gtatgcctca aaccggccgt ttatcaaccg cctaatggac 14220
tacttgcatc gcgcggccgc cgtgaacccc gagtatttca ccaatgccat cttgaacccg 14280
cactggctac cgccccctgg tttctacacc gggggattcg aggtgcccga gggtaacgat 14340
ggattcctct gggacgacat agacgacagc gtgttttccc cgcaaccgca gaccctgcta 14400
gagttgcaac agcgcgagca ggcagaggcg gcgctgcgaa aggaaagctt ccgcaggcca 14460
agcagcttgt ccgatctagg cgctgcggcc ccgcggtcag atgctagtag cccatttcca 14520
agcttgatag ggtctcttac cagcactcgc accacccgcc cgcgcctgct gggcgaggag 14580
gagtacctaa acaactcgct gctgcagccg cagcgcgaaa aaaacctgcc tccggcattt 14640
cccaacaacg ggatagagag cctagtggac aagatgagta gatggaagac gtacgcgcag 14700
gagcacaggg acgtgccagg cccgcgcccg cccacccgtc gtcaaaggca cgaccgtcag 14760
cggggtctgg tgtgggagga cgatgactcg gcagacgaca gcagcgtcct ggatttggga 14820
gggagtggca acccgtttgc gcaccttcgc cccaggctgg ggagaatgtt ttaaaaaaaa 14880
aaaagcatga tgcaaaataa aaaactcacc aaggccatgg caccgagcgt tggttttctt 14940
gtattcccct tagtatgcgg cgcgcggcga tgtatgagga aggtcctcct ccctcctacg 15000
agagtgtggt gagcgcggcg ccagtggcgg cggcgctggg ttctcccttc gatgctcccc 15060
tggacccgcc gtttgtgcct ccgcggtacc tgcggcctac cggggggaga aacagcatcc 15120
gttactctga gttggcaccc ctattcgaca ccacccgtgt gtacctggtg gacaacaagt 15180
caacggatgt ggcatccctg aactaccaga acgaccacag caactttctg accacggtca 15240
ttcaaaacaa tgactacagc ccgggggagg caagcacaca gaccatcaat cttgacgacc 15300
ggtcgcactg gggcggcgac ctgaaaacca tcctgcatac caacatgcca aatgtgaacg 15360
agttcatgtt taccaataag tttaaggcgc gggtgatggt gtcgcgcttg cctactaagg 15420
acaatcaggt ggagctgaaa tacgagtggg tggagttcac gctgcccgag ggcaactact 15480
ccgagaccat gaccatagac cttatgaaca acgcgatcgt ggagcactac ttgaaagtgg 15540
gcagacagaa cggggttctg gaaagcgaca tcggggtaaa gtttgacacc cgcaacttca 15600
gactggggtt tgaccccgtc actggtcttg tcatgcctgg ggtatataca aacgaagcct 15660
tccatccaga catcattttg ctgccaggat gcggggtgga cttcacccac agccgcctga 15720
gcaacttgtt gggcatccgc aagcggcaac ccttccagga gggctttagg atcacctacg 15780
atgatctgga gggtggtaac attcccgcac tgttggatgt ggacgcctac caggcgagct 15840
tgaaagatga caccgaacag ggcgggggtg gcgcaggcgg cagcaacagc agtggcagcg 15900
gcgcggaaga gaactccaac gcggcagccg cggcaatgca gccggtggag gacatgaacg 15960
atcatgccat tcgcggcgac acctttgcca cacgggctga ggagaagcgc gctgaggccg 16020
aagcagcggc cgaagctgcc gcccccgctg cgcaacccga ggtcgagaag cctcagaaga 16080
aaccggtgat caaacccctg acagaggaca gcaagaaacg cagttacaac ctaataagca 16140
atgacagcac cttcacccag taccgcagct ggtaccttgc atacaactac ggcgaccctc 16200
agaccggaat ccgctcatgg accctgcttt gcactcctga cgtaacctgc ggctcggagc 16260
aggtctactg gtcgttgcca gacatgatgc aagaccccgt gaccttccgc tccacgcgcc 16320
agatcagcaa ctttccggtg gtgggcgccg agctgttgcc cgtgcactcc aagagcttct 16380
acaacgacca ggccgtctac tcccaactca tccgccagtt tacctctctg acccacgtgt 16440
tcaatcgctt tcccgagaac cagattttgg cgcgcccgcc agcccccacc atcaccaccg 16500
tcagtgaaaa cgttcctgct ctcacagatc acgggacgct accgctgcgc aacagcatcg 16560
gaggagtcca gcgagtgacc attactgacg ccagacgccg cacctgcccc tacgtttaca 16620
aggccctggg catagtctcg ccgcgcgtcc tatcgagccg cactttttga gcaagcatgt 16680
ccatccttat atcgcccagc aataacacag gctggggcct gcgcttccca agcaagatgt 16740
ttggcggggc caagaagcgc tccgaccaac acccagtgcg cgtgcgcggg cactaccgcg 16800
cgccctgggg cgcgcacaaa cgcggccgca ctgggcgcac caccgtcgat gacgccatcg 16860
acgcggtggt ggaggaggcg cgcaactaca cgcccacgcc gccaccagtg tccacagtgg 16920
acgcggccat tcagaccgtg gtgcgcggag cccggcgcta tgctaaaatg aagagacggc 16980
ggaggcgcgt agcacgtcgc caccgccgcc gacccggcac tgccgcccaa cgcgcggcgg 17040
cggccctgct taaccgcgca cgtcgcaccg gccgacgggc ggccatgcgg gccgctcgaa 17100
ggctggccgc gggtattgtc actgtgcccc ccaggtccag gcgacgagcg gccgccgcag 17160
cagccgcggc cattagtgct atgactcagg gtcgcagggg caacgtgtat tgggtgcgcg 17220
actcggttag cggcctgcgc gtgcccgtgc gcacccgccc cccgcgcaac tagattgcaa 17280
gaaaaaacta cttagactcg tactgttgta tgtatccagc ggcggcggcg cgcaacgaag 17340
ctatgtccaa gcgcaaaatc aaagaagaga tgctccaggt catcgcgccg gagatctatg 17400
gccccccgaa gaaggaagag caggattaca agccccgaaa gctaaagcgg gtcaaaaaga 17460
aaaagaaaga tgatgatgat gaacttgacg acgaggtgga actgctgcac gctaccgcgc 17520
ccaggcgacg ggtacagtgg aaaggtcgac gcgtaaaacg tgttttgcga cccggcacca 17580
ccgtagtctt tacgcccggt gagcgctcca cccgcaccta caagcgcgtg tatgatgagg 17640
tgtacggcga cgaggacctg cttgagcagg ccaacgagcg cctcggggag tttgcctacg 17700
gaaagcggca taaggacatg ctggcgttgc cgctggacga gggcaaccca acacctagcc 17760
taaagcccgt aacactgcag caggtgctgc ccgcgcttgc accgtccgaa gaaaagcgcg 17820
gcctaaagcg cgagtctggt gacttggcac ccaccgtgca gctgatggta cccaagcgcc 17880
agcgactgga agatgtcttg gaaaaaatga ccgtggaacc tgggctggag cccgaggtcc 17940
gcgtgcggcc aatcaagcag gtggcgccgg gactgggcgt gcagaccgtg gacgttcaga 18000
tacccactac cagtagcacc agtattgcca ccgccacaga gggcatggag acacaaacgt 18060
ccccggttgc ctcagcggtg gcggatgccg cggtgcaggc ggtcgctgcg gccgcgtcca 18120
agacctctac ggaggtgcaa acggacccgt ggatgtttcg cgtttcagcc ccccggcgcc 18180
cgcgcggttc gaggaagtac ggcgccgcca gcgcgctact gcccgaatat gccctacatc 18240
cttccattgc gcctaccccc ggctatcgtg gctacaccta ccgccccaga agacgagcaa 18300
ctacccgacg ccgaaccacc actggaaccc gccgccgccg tcgccgtcgc cagcccgtgc 18360
tggccccgat ttccgtgcgc agggtggctc gcgaaggagg caggaccctg gtgctgccaa 18420
cagcgcgcta ccaccccagc atcgtttaaa agccggtctt tgtggttctt gcagatatgg 18480
ccctcacctg ccgcctccgt ttcccggtgc cgggattccg aggaagaatg caccgtagga 18540
ggggcatggc cggccacggc ctgacgggcg gcatgcgtcg tgcgcaccac cggcggcggc 18600
gcgcgtcgca ccgtcgcatg cgcggcggta tcctgcccct ccttattcca ctgatcgccg 18660
cggcgattgg cgccgtgccc ggaattgcat ccgtggcctt gcaggcgcag agacactgat 18720
taaaaacaag ttgcatgtgg aaaaatcaaa ataaaaagtc tggactctca cgctcgcttg 18780
gtcctgtaac tattttgtag aatggaagac atcaactttg cgtctctggc cccgcgacac 18840
ggctcgcgcc cgttcatggg aaactggcaa gatatcggca ccagcaatat gagcggtggc 18900
gccttcagct ggggctcgct gtggagcggc attaaaaatt tcggttccac cgttaagaac 18960
tatggcagca aggcctggaa cagcagcaca ggccagatgc tgagggataa gttgaaagag 19020
caaaatttcc aacaaaaggt ggtagatggc ctggcctctg gcattagcgg ggtggtggac 19080
ctggccaacc aggcagtgca aaataagatt aacagtaagc ttgatccccg ccctcccgta 19140
gaggagcctc caccggccgt ggagacagtg tctccagagg ggcgtggcga aaagcgtccg 19200
cgccccgaca gggaagaaac tctggtgacg caaatagacg agcctccctc gtacgaggag 19260
gcactaaagc aaggcctgcc caccacccgt cccatcgcgc ccatggctac cggagtgctg 19320
ggccagcaca cacccgtaac gctggacctg cctccccccg ccgacaccca gcagaaacct 19380
gtgctgccag gcccgaccgc cgttgttgta acccgtccta gccgcgcgtc cctgcgccgc 19440
gccgccagcg gtccgcgatc gttgcggccc gtagccagtg gcaactggca aagcacactg 19500
aacagcatcg tgggtctggg ggtgcaatcc ctgaagcgcc gacgatgctt ctgaatagct 19560
aacgtgtcgt atgtgtgtca tgtatgcgtc catgtcgccg ccagaggagc tgctgagccg 19620
ccgcgcgccc gctttccaag atggctaccc cttcgatgat gccgcagtgg tcttacatgc 19680
acatctcggg ccaggacgcc tcggagtacc tgagccccgg gctggtgcag tttgcccgcg 19740
ccaccgagac gtacttcagc ctgaataaca agtttagaaa ccccacggtg gcgcctacgc 19800
acgacgtgac cacagaccgg tcccagcgtt tgacgctgcg gttcatccct gtggaccgtg 19860
aggatactgc gtactcgtac aaggcgcggt tcaccctagc tgtgggtgat aaccgtgtgc 19920
tggacatggc ttccacgtac tttgacatcc gcggcgtgct ggacaggggc cctactttta 19980
agccctactc tggcactgcc tacaacgccc tggctcccaa gggtgcccca aatccttgcg 20040
aatgggatga agctgctact gctcttgaaa taaacctaga agaagaggac gatgacaacg 20100
aagacgaagt agacgagcaa gctgagcagc aaaaaactca cgtatttggg caggcgcctt 20160
attctggtat aaatattaca aaggagggta ttcaaatagg tgtcgaaggt caaacaccta 20220
aatatgccga taaaacattt caacctgaac ctcaaatagg agaatctcag tggtacgaaa 20280
ctgaaattaa tcatgcagct gggagagtcc ttaaaaagac taccccaatg aaaccatgtt 20340
acggttcata tgcaaaaccc acaaatgaaa atggagggca aggcattctt gtaaagcaac 20400
aaaatggaaa gctagaaagt caagtggaaa tgcaattttt ctcaactact gaggcgaccg 20460
caggcaatgg tgataacttg actcctaaag tggtattgta cagtgaagat gtagatatag 20520
aaaccccaga cactcatatt tcttacatgc ccactattaa ggaaggtaac tcacgagaac 20580
taatgggcca acaatctatg cccaacaggc ctaattacat tgcttttagg gacaatttta 20640
ttggtctaat gtattacaac agcacgggta atatgggtgt tctggcgggc caagcatcgc 20700
agttgaatgc tgttgtagat ttgcaagaca gaaacacaga gctttcatac cagcttttgc 20760
ttgattccat tggtgataga accaggtact tttctatgtg gaatcaggct gttgacagct 20820
atgatccaga tgttagaatt attgaaaatc atggaactga agatgaactt ccaaattact 20880
gctttccact gggaggtgtg attaatacag agactcttac caaggtaaaa cctaaaacag 20940
gtcaggaaaa tggatgggaa aaagatgcta cagaattttc agataaaaat gaaataagag 21000
ttggaaataa ttttgccatg gaaatcaatc taaatgccaa cctgtggaga aatttcctgt 21060
actccaacat agcgctgtat ttgcccgaca agctaaagta cagtccttcc aacgtaaaaa 21120
tttctgataa cccaaacacc tacgactaca tgaacaagcg agtggtggct cccgggttag 21180
tggactgcta cattaacctt ggagcacgct ggtcccttga ctatatggac aacgtcaacc 21240
catttaacca ccaccgcaat gctggcctgc gctaccgctc aatgttgctg ggcaatggtc 21300
gctatgtgcc cttccacatc caggtgcctc agaagttctt tgccattaaa aacctccttc 21360
tcctgccggg ctcatacacc tacgagtgga acttcaggaa ggatgttaac atggttctgc 21420
agagctccct aggaaatgac ctaagggttg acggagccag cattaagttt gatagcattt 21480
gcctttacgc caccttcttc cccatggccc acaacaccgc ctccacgctt gaggccatgc 21540
ttagaaacga caccaacgac cagtccttta acgactatct ctccgccgcc aacatgctct 21600
accctatacc cgccaacgct accaacgtgc ccatatccat cccctcccgc aactgggcgg 21660
ctttccgcgg ctgggccttc acgcgcctta agactaagga aaccccatca ctgggctcgg 21720
gctacgaccc ttattacacc tactctggct ctatacccta cctagatgga accttttacc 21780
tcaaccacac ctttaagaag gtggccatta cctttgactc ttctgtcagc tggcctggca 21840
atgaccgcct gcttaccccc aacgagtttg aaattaagcg ctcagttgac ggggagggtt 21900
acaacgttgc ccagtgtaac atgaccaaag actggttcct ggtacaaatg ctagctaact 21960
acaacattgg ctaccagggc ttctatatcc cagagagcta caaggaccgc atgtactcct 22020
tctttagaaa cttccagccc atgagccgtc aggtggtgga tgatactaaa tacaaggact 22080
accaacaggt gggcatccta caccaacaca acaactctgg atttgttggc taccttgccc 22140
ccaccatgcg cgaaggacag gcctaccctg ctaacttccc ctatccgctt ataggcaaga 22200
ccgcagttga cagcattacc cagaaaaagt ttctttgcga tcgcaccctt tggcgcatcc 22260
cattctccag taactttatg tccatgggcg cactcacaga cctgggccaa aaccttctct 22320
acgccaactc cgcccacgcg ctagacatga cttttgaggt ggatcccatg gacgagccca 22380
cccttcttta tgttttgttt gaagtctttg acgtggtccg tgtgcaccgg ccgcaccgcg 22440
gcgtcatcga aaccgtgtac ctgcgcacgc ccttctcggc cggcaacgcc acaacataaa 22500
gaagcaagca acatcaacaa cagctgccgc catgggctcc agtgagcagg aactgaaagc 22560
cattgtcaaa gatcttggtt gtgggccata ttttttgggc acctatgaca agcgctttcc 22620
aggctttgtt tctccacaca agctcgcctg cgccatagtc aatacggccg gtcgcgagac 22680
tgggggcgta cactggatgg cctttgcctg gaacccgcac tcaaaaacat gctacctctt 22740
tgagcccttt ggcttttctg accagcgact caagcaggtt taccagtttg agtacgagtc 22800
actcctgcgc cgtagcgcca ttgcttcttc ccccgaccgc tgtataacgc tggaaaagtc 22860
cacccaaagc gtacaggggc ccaactcggc cgcctgtgga ctattctgct gcatgtttct 22920
ccacgccttt gccaactggc cccaaactcc catggatcac aaccccacca tgaaccttat 22980
taccggggta cccaactcca tgctcaacag tccccaggta cagcccaccc tgcgtcgcaa 23040
ccaggaacag ctctacagct tcctggagcg ccactcgccc tacttccgca gccacagtgc 23100
gcagattagg agcgccactt ctttttgtca cttgaaaaac atgtaaaaat aatgtactag 23160
agacactttc aataaaggca aatgctttta tttgtacact ctcgggtgat tatttacccc 23220
cacccttgcc gtctgcgccg tttaaaaatc aaaggggttc tgccgcgcat cgctatgcgc 23280
cactggcagg gacacgttgc gatactggtg tttagtgctc cacttaaact caggcacaac 23340
catccgcggc agctcggtga agttttcact ccacaggctg cgcaccatca ccaacgcgtt 23400
tagcaggtcg ggcgccgata tcttgaagtc gcagttgggg cctccgccct gcgcgcgcga 23460
gttgcgatac acagggttgc agcactggaa cactatcagc gccgggtggt gcacgctggc 23520
cagcacgctc ttgtcggaga tcagatccgc gtccaggtcc tccgcgttgc tcagggcgaa 23580
cggagtcaac tttggtagct gccttcccaa aaagggcgcg tgcccaggct ttgagttgca 23640
ctcgcaccgt agtggcatca aaaggtgacc gtgcccggtc tgggcgttag gatacagcgc 23700
ctgcataaaa gccttgatct gcttaaaagc cacctgagcc tttgcgcctt cagagaagaa 23760
catgccgcaa gacttgccgg aaaactgatt ggccggacag gccgcgtcgt gcacgcagca 23820
ccttgcgtcg gtgttggaga tctgcaccac atttcggccc caccggttct tcacgatctt 23880
ggccttgcta gactgctcct tcagcgcgcg ctgcccgttt tcgctcgtca catccatttc 23940
aatcacgtgc tccttattta tcataatgct tccgtgtaga cacttaagct cgccttcgat 24000
ctcagcgcag cggtgcagcc acaacgcgca gcccgtgggc tcgtgatgct tgtaggtcac 24060
ctctgcaaac gactgcaggt acgcctgcag gaatcgcccc atcatcgtca caaaggtctt 24120
gttgctggtg aaggtcagct gcaacccgcg gtgctcctcg ttcagccagg tcttgcatac 24180
ggccgccaga gcttccactt ggtcaggcag tagtttgaag ttcgccttta gatcgttatc 24240
cacgtggtac ttgtccatca gcgcgcgcgc agcctccatg cccttctccc acgcagacac 24300
gatcggcaca ctcagcgggt tcatcaccgt aatttcactt tccgcttcgc tgggctcttc 24360
ctcttcctct tgcgtccgca taccacgcgc cactgggtcg tcttcattca gccgccgcac 24420
tgtgcgctta cctcctttgc catgcttgat tagcaccggt gggttgctga aacccaccat 24480
ttgtagcgcc acatcttctc tttcttcctc gctgtccacg attacctctg gtgatggcgg 24540
gcgctcgggc ttgggagaag ggcgcttctt tttcttcttg ggcgcaatgg ccaaatccgc 24600
cgccgaggtc gatggccgcg ggctgggtgt gcgcggcacc agcgcgtctt gtgatgagtc 24660
ttcctcgtcc tcggactcga tacgccgcct catccgcttt tttgggggcg cccggggagg 24720
cggcggcgac ggggacgggg acgacacgtc ctccatggtt gggggacgtc gcgccgcacc 24780
gcgtccgcgc tcgggggtgg tttcgcgctg ctcctcttcc cgactggcca tttccttctc 24840
ctataggcag aaaaagatca tggagtcagt cgagaagaag gacagcctaa ccgccccctc 24900
tgagttcgcc accaccgcct ccaccgatgc cgccaacgcg cctaccacct tccccgtcga 24960
ggcacccccg cttgaggagg aggaagtgat tatcgagcag gacccaggtt ttgtaagcga 25020
agacgacgag gaccgctcag taccaacaga ggataaaaag caagaccagg acaacgcaga 25080
ggcaaacgag gaacaagtcg ggcgggggga cgaaaggcat ggcgactacc tagatgtggg 25140
agacgacgtg ctgttgaagc atctgcagcg ccagtgcgcc attatctgcg acgcgttgca 25200
agagcgcagc gatgtgcccc tcgccatagc ggatgtcagc cttgcctacg aacgccacct 25260
attctcaccg cgcgtacccc ccaaacgcca agaaaacggc acatgcgagc ccaacccgcg 25320
cctcaacttc taccccgtat ttgccgtgcc agaggtgctt gccacctatc acatcttttt 25380
ccaaaactgc aagatacccc tatcctgccg tgccaaccgc agccgagcgg acaagcagct 25440
ggccttgcgg cagggcgctg tcatacctga tatcgcctcg ctcaacgaag tgccaaaaat 25500
ctttgagggt cttggacgcg acgagaagcg cgcggcaaac gctctgcaac aggaaaacag 25560
cgaaaatgaa agtcactctg gagtgttggt ggaactcgag ggtgacaacg cgcgcctagc 25620
cgtactaaaa cgcagcatcg aggtcaccca ctttgcctac ccggcactta acctaccccc 25680
caaggtcatg agcacagtca tgagtgagct gatcgtgcgc cgtgcgcagc ccctggagag 25740
ggatgcaaat ttgcaagaac aaacagagga gggcctaccc gcagttggcg acgagcagct 25800
agcgcgctgg cttcaaacgc gcgagcctgc cgacttggag gagcgacgca aactaatgat 25860
ggccgcagtg ctcgttaccg tggagcttga gtgcatgcag cggttctttg ctgacccgga 25920
gatgcagcgc aagctagagg aaacattgca ctacaccttt cgacagggct acgtacgcca 25980
ggcctgcaag atctccaacg tggagctctg caacctggtc tcctaccttg gaattttgca 26040
cgaaaaccgc cttgggcaaa acgtgcttca ttccacgctc aagggcgagg cgcgccgcga 26100
ctacgtccgc gactgcgttt acttatttct atgctacacc tggcagacgg ccatgggcgt 26160
ttggcagcag tgcttggagg agtgcaacct caaggagctg cagaaactgc taaagcaaaa 26220
cttgaaggac ctatggacgg ccttcaacga gcgctccgtg gccgcgcacc tggcggacat 26280
cattttcccc gaacgcctgc ttaaaaccct gcaacagggt ctgccagact tcaccagtca 26340
aagcatgttg cagaacttta ggaactttat cctagagcgc tcaggaatct tgcccgccac 26400
ctgctgtgca cttcctagcg actttgtgcc cattaagtac cgcgaatgcc ctccgccgct 26460
ttggggccac tgctaccttc tgcagctagc caactacctt gcctaccact ctgacataat 26520
ggaagacgtg agcggtgacg gtctactgga gtgtcactgt cgctgcaacc tatgcacccc 26580
gcaccgctcc ctggtttgca attcgcagct gcttaacgaa agtcaaatta tcggtacctt 26640
tgagctgcag ggtccctcgc ctgacgaaaa gtccgcggct ccggggttga aactcactcc 26700
ggggctgtgg acgtcggctt accttcgcaa atttgtacct gaggactacc acgcccacga 26760
gattaggttc tacgaagacc aatcccgccc gccaaatgcg gagcttaccg cctgcgtcat 26820
tacccagggc cacattcttg gccaattgca agccatcaac aaagcccgcc aagagtttct 26880
gctacgaaag ggacgggggg tttacttgga cccccagtcc ggcgaggagc tcaacccaat 26940
ccccccgccg ccgcagccct atcagcagca gccgcgggcc cttgcttccc aggatggcac 27000
ccaaaaagaa gctgcagctg ccgccgccac ccacggacga ggaggaatac tgggacagtc 27060
aggcagagga ggttttggac gaggaggagg aggacatgat ggaagactgg gagagcctag 27120
acgaggaagc ttccgaggtc gaagaggtgt cagacgaaac accgtcaccc tcggtcgcat 27180
tcccctcgcc ggcgccccag aaatcggcaa ccggttccag catggctaca acctccgctc 27240
ctcaggcgcc gccggcactg cccgttcgcc gacccaaccg tagatgggac accactggaa 27300
ccagggccgg taagtccaag cagccgccgc cgttagccca agagcaacaa cagcgccaag 27360
gctaccgctc atggcgcggg cacaagaacg ccatagttgc ttgcttgcaa gactgtgggg 27420
gcaacatctc cttcgcccgc cgctttcttc tctaccatca cggcgtggcc ttcccccgta 27480
acatcctgca ttactaccgt catctctaca gcccatactg caccggcggc agcggcagcg 27540
gcagcaacag cagcggccac acagaagcaa aggcgaccgg atagcaagac tctgacaaag 27600
cccaagaaat ccacagcggc ggcagcagca ggaggaggag cgctgcgtct ggcgcccaac 27660
gaacccgtat cgacccgcga gcttagaaac aggatttttc ccactctgta tgctatattt 27720
caacagagca ggggccaaga acaagagctg aaaataaaaa acaggtctct gcgatccctc 27780
acccgcagct gcctgtatca caaaagcgaa gatcagcttc ggcgcacgct ggaagacgcg 27840
gaggctctct tcagtaaata ctgcgcgctg actcttaagg actagtttcg cgccctttct 27900
caaatttaag cgcgaaaact acgtcatctc cagcggccac acccggcgcc agcacctgtc 27960
gtcagcgcca tttcaacttt gtatacaaaa gttgtgatga gcaaggaaat tcccacgccc 28020
tacatgtgga gttaccagcc acaaatggga cttgcggctg gagctgccca agactactca 28080
acccgaataa actacatgag cgcgggaccc cacatgatat cccgggtcaa cggaatacgc 28140
gcccaccgaa accgaattct cctggaacag gcggctatta ccaccacacc tcgtaataac 28200
cttaatcccc gtagttggcc cgctgccctg gtgtaccagg aaagtcccgc tcccaccact 28260
gtggtacttc ccagagacgc ccaggccgaa gttcagatga ctaactcagg ggcgcagctt 28320
gcgggcggct ttcgtcacag ggtgcggtcg cccgggcagg gtataactca cctgacaatc 28380
agagggcgag gtattcagct caacgacgag tcggtgagct cctcgcttgg tctccgtccg 28440
gacgggacat ttcagatcgg cggcgccggc cgctcttcat tcacgcctcg tcaggcaatc 28500
ctaactctgc agacctcgtc ctctgagccg cgctctggag gcattggaac tctgcaattt 28560
attgaggagt ttgtgccatc ggtctacttt aaccccttct cgggacctcc cggccactat 28620
ccggatcaat ttattcctaa ctttgacgcg gtaaaggact cggcggacgg ctacgactga 28680
atgttaagtg gagaggcaga gcaactgcgc ctgaaacacc tggtccactg tcgccgccac 28740
aagtgctttg cccgcgactc cggtgagttt tgctactttg aattgcccga ggatcatatc 28800
gagggcccgg cgcacggcgt ccggcttacc gcccagggag agcttgcccg tagcctgatt 28860
cgggagttta cccagcgccc cctgctagtt gagcgggaca ggggaccctg tgttctcact 28920
gtgatttgca actgtcctaa ccctggatta catcaagatc tttgttgcca tctctgtgct 28980
gagtataata aatacagaaa ttaaaatata ctggggctcc tatcgccatc ctgtaaacgc 29040
caccgtcttc acccgcccaa gcaaaccaag gcgaacctta cctggtactt ttaacatctc 29100
tccctctgtg atttacaaca gtttcaaccc agacggagtg agtctacgag agaacctctc 29160
cgagctcagc tactccatca gaaaaaacac caccctcctt acctgccggg aacgtacgag 29220
tgcgtcaccg gccgctgcac cacacctacc gcctgaccgt aaaccagact ttttccggac 29280
agacctcaat aactctgttt accagaacag gaggtgagct tagaaaaccc ttagggtatt 29340
aggccaaagg cgcagctact gtggggttta tgaacaattc aagcaactct acgggctatt 29400
ctaattcagg tttctctaga atcggggttg gggttattct ctgtcttgtg attctcttta 29460
ttcttatact aacgcttctc tgcctaaggc tcgccgcctg ctgtgtgcac atttgcattt 29520
attgtcagct ttttaaacgc tggggtcgcc acccaagatg attaggtaca taatcctagg 29580
tttactcacc cttgcgtcag cccacggtac cacccaaaag gtggatttta aggagccagc 29640
ctgtaatgtt acattcgcag ctgaagctaa tgagtgcacc actcttataa aatgcaccac 29700
agaacatgaa aagctgctta ttcgccacaa aaacaaaatt ggcaagtatg ctgtttatgc 29760
tatttggcag ccaggtgaca ctacagagta taatgttaca gttttccagg gtaaaagtca 29820
taaaactttt atgtatactt ttccatttta tgaaatgtgc gacattacca tgtacatgag 29880
caaacagtat aagttgtggc ccccacaaaa ttgtgtggaa aacactggca ctttctgctg 29940
cactgctatg ctaattacag tgctcgcttt ggtctgtacc ctactctata ttaaatacaa 30000
aagcagacgc agctttattg aggaaaagaa aatgccttaa tttactaagt tacaaagcta 30060
atgtcaccac taactgcttt actcgctgct tgcaaaacaa attcaaaaag ttagcattat 30120
aattagaata ggatttaaac cccccggtca tttcctgctc aataccattc ccctgaacaa 30180
ttgactctat gtgggatatg ctccagcgct acaaccttga agtcaggctt cctggatgtc 30240
agcatctgac tttggccagc acctgtcccg cggatttgtt ccagtccaac tacagcgacc 30300
caccctaaca gagatgacca acacaaccaa cgcggccgcc gctaccggac ttacatctac 30360
cacaaataca ccccaagttt ctgcctttgt caataactgg gataacttgg gcatgtggtg 30420
gttctccata gcgcttatgt ttgtatgcct tattattatg tggctcatct gctgcctaaa 30480
gcgcaaacgc gcccgaccac ccatctatag tcccatcatt gtgctacacc caaacaatga 30540
tggaatccat agattggacg gactgaaaca catgttcttt tctcttacag tatgattaaa 30600
tgagacatga ttcctcgagt ttttatatta ctgacccttg ttgcgctttt ttgtgcgtgc 30660
tccacattgg ctgcggtttc tcacatcgaa gtagactgca ttccagcctt cacagtctat 30720
ttgctttacg gatttgtcac cctcacgctc atctgcagcc tcatcactgt ggtcatcgcc 30780
tttatccagt gcattgactg ggtctgtgtg cgctttgcat atctcagaca ccatccccag 30840
tacagggaca ggactatagc tgagcttctt agaattcttt aattatgaaa tttactgtga 30900
cttttctgct gattatttgc accctatctg cgttttgttc cccgacctcc aagcctcaaa 30960
gacatatatc atgcagattc actcgtatat ggaatattcc aagttgctac aatgaaaaaa 31020
gcgatctttc cgaagcctgg ttatatgcaa tcatctctgt tatggtgttc tgcagtacca 31080
tcttagccct agctatatat ccctaccttg acattggctg gaacgcaata gatgccatga 31140
accacccaac tttccccgcg cccgctatgc ttccactgca acaagttgtt gccggcggct 31200
ttgtcccagc caatcagcct cgcccacctt ctcccacccc cactgaaatc agctacttta 31260
atctaacagg aggagatgac tgacacccta gatctagaaa tggacggaat tattacagag 31320
cagcgcctgc tagaaagacg cagggcagcg gccgagcaac agcgcatgaa tcaagagctc 31380
caagacatgg ttaacttgca ccagtgcaaa aggggtatct tttgtctggt aaagcaggcc 31440
aaagtcacct acgacagtaa taccaccgga caccgcctta gctacaagtt gccaaccaag 31500
cgtcagaaat tggtggtcat ggtgggagaa aagcccatta ccataactca gcactcggta 31560
gaaaccgaag gctgcattca ctcaccttgt caaggacctg aggatctctg cacccttatt 31620
aagaccctgt gcggtctcaa agatcttatt ccctttaact aataaaaaaa aataataaag 31680
catcacttac ttaaaatcag ttagcaaatt tctgtccagt ttattcagca gcacctcctt 31740
gccctcctcc cagctctggt attgcagctt cctcctggct gcaaactttc tccacaatct 31800
aaatggaatg tcagtttcct cctgttcctg tccatccgca cccactatct tcatgttgtt 31860
gcagatgaag cgcgcaagac cgtctgaaga taccttcaac cccgtgtatc catatgacac 31920
ggaaaccggt cctccaactg tgccttttct tactcctccc tttgtatccc ccaatgggtt 31980
tcaagagagt ccccctgggg tactctcttt gcgcctatcc gaacctctag ttacctccaa 32040
tggcatgctt gcgctcaaaa tgggcaacgg cctctctctg gacgaggccg gcaaccttac 32100
ctcccaaaat gtaaccactg tgagcccacc tctcaaaaaa accaagtcaa acataaacct 32160
ggaaatatct gcacccctca cagttacctc agaagcccta actgtggctg ccgccgcacc 32220
tctaatggtc gcgggcaaca cactcaccat gcaatcacag gccccgctaa ccgtgcacga 32280
ctccaaactt agcattgcca cccaaggacc cctcacagtg tcagaaggaa agctagccct 32340
gcaaacatca ggccccctca ccaccaccga tagcagtacc cttactatca ctgcctcacc 32400
ccctctaact actgccactg gtagcttggg cattgacttg aaagagccca tttatacaca 32460
aaatggaaaa ctaggactaa agtacggggc tcctttgcat gtaacagacg acctaaacac 32520
tttgaccgta gcaactggtc caggtgtgac tattaataat acttccttgc aaactaaagt 32580
tactggagcc ttgggttttg attcacaagg caatatgcaa cttaatgtag caggaggact 32640
aaggattgat tctcaaaaca gacgccttat acttgatgtt agttatccgt ttgatgctca 32700
aaaccaacta aatctaagac taggacaggg ccctcttttt ataaactcag cccacaactt 32760
ggatattaac tacaacaaag gcctttactt gtttacagct tcaaacaatt ccaaaaagct 32820
tgaggttaac ctaagcactg ccaaggggtt gatgtttgac gctacagcca tagccattaa 32880
tgcaggagat gggcttgaat ttggttcacc taatgcacca aacacaaatc ccctcaaaac 32940
aaaaattggc catggcctag aatttgattc aaacaaggct atggttccta aactaggaac 33000
tggccttagt tttgacagca caggtgccat tacagtagga aacaaaaata atgataagct 33060
aactttgtgg accacaccag ctccatctcc taactgtaga ctaaatgcag agaaagatgc 33120
taaactcact ttggtcttaa caaaatgtgg cagtcaaata cttgctacag tttcagtttt 33180
ggctgttaaa ggcagtttgg ctccaatatc tggaacagtt caaagtgctc atcttattat 33240
aagatttgac gaaaatggag tgctactaaa caattccttc ctggacccag aatattggaa 33300
ctttagaaat ggagatctta ctgaaggcac agcctataca aacgctgttg gatttatgcc 33360
taacctatca gcttatccaa aatctcacgg taaaactgcc aaaagtaaca ttgtcagtca 33420
agtttactta aacggagaca aaactaaacc tgtaacacta accattacac taaacggtac 33480
acaggaaaca ggagacacaa ctccaagtgc atactctatg tcattttcat gggactggtc 33540
tggccacaac tacattaatg aaatatttgc cacatcctct tacacttttt catacattgc 33600
ccaagaataa agaatcgttt gtgttatgtt tcaacgtgtt tatttttcaa ttgcagaaaa 33660
tttcaagtca tttttcattc agtagtatag ccccaccacc acatagctta tacagatcac 33720
cgtacctcaa ctttgtataa taaagttgta atcaaactca cagaacccta gtattcaacc 33780
tgccacctcc ctcccaacac acagagtaca cagtcctttc tccccggctg gccttaaaaa 33840
gcatcatatc atgggtaaca gacatattct taggtgttat attccacacg gtttcctgtc 33900
gagccaaacg ctcatcagtg atattaataa actccccggg cagctcactt aagttcatgt 33960
cgctgtccag ctgctgagcc acaggctgct gtccaacttg cggttgctta acgggcggcg 34020
aaggagaagt ccacgcctac atgggggtag agtcataatc gtgcatcagg atagggcggt 34080
ggtgctgcag cagcgcgcga ataaactgct gccgccgccg ctccgtcctg caggaataca 34140
acatggcagt ggtctcctca gcgatgattc gcaccgcccg cagcataagg cgccttgtcc 34200
tccgggcaca gcagcgcacc ctgatctcac ttaaatcagc acagtaactg cagcacagca 34260
ccacaatatt gttcaaaatc ccacagtgca aggcgctgta tccaaagctc atggcgggga 34320
ccacagaacc cacgtggcca tcataccaca agcgcaggta gattaagtgg cgacccctca 34380
taaacacgct ggacataaac attacctctt ttggcatgtt gtaattcacc acctcccggt 34440
accatataaa cctctgatta aacatggcgc catccaccac catcctaaac cagctggcca 34500
aaacctgccc gccggctata cactgcaggg aaccgggact ggaacaatga cagtggagag 34560
cccaggactc gtaaccatgg atcatcatgc tcgtcatgat atcaatgttg gcacaacaca 34620
ggcacacgtg catacacttc ctcaggatta caagctcctc ccgcgttaga accatatccc 34680
agggaacaac ccattcctga atcagcgtaa atcccacact gcagggaaga cctcgcacgt 34740
aactcacgtt gtgcattgtc aaagtgttac attcgggcag cagcggatga tcctccagta 34800
tggtagcgcg ggtttctgtc tcaaaaggag gtagacgatc cctactgtac ggagtgcgcc 34860
gagacaaccg agatcgtgtt ggtcgtagtg tcatgccaaa tggaacgccg gacgtagtca 34920
tatttcctga agcaaaacca ggtgcgggcg tgacaaacag atctgcgtct ccggtctcgc 34980
cgcttagatc gctctgtgta gtagttgtag tatatccact ctctcaaagc atccaggcgc 35040
cccctggctt cgggttctat gtaaactcct tcatgcgccg ctgccctgat aacatccacc 35100
accgcagaat aagccacacc cagccaacct acacattcgt tctgcgagtc acacacggga 35160
ggagcgggaa gagctggaag aaccatgttt ttttttttat tccaaaagat tatccaaaac 35220
ctcaaaatga agatctatta agtgaacgcg ctcccctccg gtggcgtggt caaactctac 35280
agccaaagaa cagataatgg catttgtaag atgttgcaca atggcttcca aaaggcaaac 35340
ggccctcacg tccaagtgga cgtaaaggct aaacccttca gggtgaatct cctctataaa 35400
cattccagca ccttcaacca tgcccaaata attctcatct cgccaccttc tcaatatatc 35460
tctaagcaaa tcccgaatat taagtccggc cattgtaaaa atctgctcca gagcgccctc 35520
caccttcagc ctcaagcagc gaatcatgat tgcaaaaatt caggttcctc acagacctgt 35580
ataagattca aaagcggaac attaacaaaa ataccgcgat cccgtaggtc ccttcgcagg 35640
gccagctgaa cataatcgtg caggtctgca cggaccagcg cggccacttc cccgccagga 35700
accatgacaa aagaacccac actgattatg acacgcatac tcggagctat gctaaccagc 35760
gtagccccga tgtaagcttg ttgcatgggc ggcgatataa aatgcaaggt gctgctcaaa 35820
aaatcaggca aagcctcgcg caaaaaagaa agcacatcgt agtcatgctc atgcagataa 35880
aggcaggtaa gctccggaac caccacagaa aaagacacca tttttctctc aaacatgtct 35940
gcgggtttct gcataaacac aaaataaaat aacaaaaaaa catttaaaca ttagaagcct 36000
gtcttacaac aggaaaaaca acccttataa gcataagacg gactacggcc atgccggcgt 36060
gaccgtaaaa aaactggtca ccgtgattaa aaagcaccac cgacagctcc tcggtcatgt 36120
ccggagtcat aatgtaagac tcggtaaaca catcaggttg attcacatcg gtcagtgcta 36180
aaaagcgacc gaaatagccc gggggaatac atacccgcag gcgtagagac aacattacag 36240
cccccatagg aggtataaca aaattaatag gagagaaaaa cacataaaca cctgaaaaac 36300
cctcctgcct aggcaaaata gcaccctccc gctccagaac aacatacagc gcttccacag 36360
cggcagccat aacagtcagc cttaccagta aaaaagaaaa cctattaaaa aaacaccact 36420
cgacacggca ccagctcaat cagtcacagt gtaaaaaagg gccaagtgca gagcgagtat 36480
atataggact aaaaaatgac gtaacggtta aagtccacaa aaaacaccca gaaaaccgca 36540
cgcgaaccta cgcccagaaa cgaaagccaa aaaacccaca acttcctcaa atcgtcactt 36600
ccgttttccc acgttacgtc acttcccatt ttaagaaaac tacaattccc aacacataca 36660
agttactccg ccctaaaacc tacgtcaccc gccccgttcc cacgccccgc gccacgtcac 36720
aaactccacc ccctcattat catattggct tcaatccaaa ataaggtata ttattgatga 36780
tg 36782
<210> 5
<211> 36794
<212> DNA
<213> 人工序列
<220>
<223> 合成腺病毒基因组YPet-P2A-(DNA Poly)
<400> 5
catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60
ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120
gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180
gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240
taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300
agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360
gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420
cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480
tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540
tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600
aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660
tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720
cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780
gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840
cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900
ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960
cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020
caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080
ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140
tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200
gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260
ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320
cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380
ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440
gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500
cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560
ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620
gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680
cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740
ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800
tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860
gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920
caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980
gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040
agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100
aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160
cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220
gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280
gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340
gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400
gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460
tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520
ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580
tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640
agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700
tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760
gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820
acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880
gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940
aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000
ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060
gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120
tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180
acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240
aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300
tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360
gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420
tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480
ttggctctag cgatgaagat acagattgag gtactgaaat gtgtgggcgt ggcttaaggg 3540
tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg 3600
ccgccgccca acttttctat acaaagttgt aatgagcacc aactcgtttg atggaagcat 3660
tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat 3720
gggctccagc attgatggtc gccccgtcct gcccgcaaac tctactacct tgacctacga 3780
gaccgtgtct ggaacgccgt tggagactgc agcctccgcc gccgcttcag ccgctgcagc 3840
caccgcccgc gggattgtga ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc 3900
ttcccgttca tccgcccgcg atgacaagtt gacggctctt ttggcacaat tggattcttt 3960
gacccgggaa cttaatgtcg tttctcagca gctgttggat ctgcgccagc aggtttctgc 4020
cctgaaggct tcctcccctc ccaatgcggt ttaaaacata aataaaaaac cagactctgt 4080
ttggatttgg atcaagcata agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg 4140
taggcccggg accagcggtc tcggtcgttg agggtcctgt gtattttttc caggacgtgg 4200
taaaggtgac tctggatgtt cagatacatg ggcataagcc cgtctctggg gtggaggtag 4260
caccactgca gagcttcatg ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag 4320
cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc tgattgccag gggcaggccc 4380
ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt gcatacgtgg ggatatgaga 4440
tgcatcttgg actgtatttt taggttggct atgttcccag ccatatccct ccggggattc 4500
atgttgtgca gaaccaccag cacagtgtat ccggtgcact tgggaaattt gtcatgtagc 4560
ttagaaggaa atgcgtggaa gaacttggag acgcccttgt gacctccaag attttccatg 4620
cattcgtcca taatgatggc aatgggccca cgggcggcgg cctgggcgaa gatatttctg 4680
ggatcactaa cgtcatagtt gtgttccagg atgagatcgt cataggccat ttttacaaag 4740
cgcgggcgga gggtgccaga ctgcggtata atggttccat ccggcccagg ggcgtagtta 4800
ccctcacaga tttgcatttc ccacgctttg agttcagatg gggggatcat gtctacctgc 4860
ggggcgatga agaaaacggt ttccggggta ggggagatca gctgggaaga aagcaggttc 4920
ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa tcacacctat taccgggtgc 4980
aactggtagt taagagagct gcagctgccg tcatccctga gcaggggggc cacttcgtta 5040
agcatgtccc tgactcgcat gttttccctg accaaatccg ccagaaggcg ctcgccgccc 5100
agcgatagca gttcttgcaa ggaagcaaag tttttcaacg gtttgagacc gtccgccgta 5160
ggcatgcttt tgagcgtttg accaagcagt tccaggcggt cccacagctc ggtcacctgc 5220
tctacggcat ctcgatccag catatctcct cgtttcgcgg gttggggcgg ctttcgctgt 5280
acggcagtag tcggtgctcg tccagacggg ccagggtcat gtctttccac gggcgcaggg 5340
tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc tccgggctgc gcgctggcca 5400
gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt 5460
cggccaggta gcatttgacc atggtgtcat agtccagccc ctccgcggcg tggcccttgg 5520
cgcgcagctt gcccttggag gaggcgccgc acgaggggca gtgcagactt ttgagggcgt 5580
agagcttggg cgcgagaaat accgattccg gggagtaggc atccgcgccg caggccccgc 5640
agacggtctc gcattccacg agccaggtga gctctggccg ttcggggtca aaaaccaggt 5700
ttcccccatg ctttttgatg cgtttcttac ctctggtttc catgagccgg tgtccacgct 5760
cggtgacgaa aaggctgtcc gtgtccccgt atacagactt gagaggcctg tcctcgagcg 5820
gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacaaag gctcgcgtcc 5880
aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact agggggtcca 5940
ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag gtgattggtt 6000
tgtaggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag ggggtggggg 6060
cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt tggggtgagt 6120
actccctctg aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg 6180
aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgca tccatctggt 6240
cagaaaagac aatctttttg ttgtcaagct tggtggcaaa cgacccgtag agggcgttgg 6300
acagcaactt ggcgatggag cgcagggttt ggtttttgtc gcgatcggcg cgctccttgg 6360
ccgcgatgtt tagctgcacg tattcgcgcg caacgcaccg ccattcggga aagacggtgg 6420
tgcgctcgtc gggcaccagg tgcacgcgcc aaccgcggtt gtgcagggtg acaaggtcaa 6480
cgctggtggc tacctctccg cgtaggcgct cgttggtcca gcagaggcgg ccgcccttgc 6540
gcgagcagaa tggcggtagg gggtctagct gcgtctcgtc cggggggtct gcgtccacgg 6600
taaagacccc gggcagcagg cgcgcgtcga agtagtctat cttgcatcct tgcaagtcta 6660
gcgcctgctg ccatgcgcgg gcggcaagcg cgcgctcgta tgggttgagt gggggacccc 6720
atggcatggg gtgggtgagc gcggaggcgt acatgccgca aatgtcgtaa acgtagaggg 6780
gctctctgag tattccaaga tatgtagggt agcatcttcc accgcggatg ctggcgcgca 6840
cgtaatcgta tagttcgtgc gagggagcga ggaggtcggg accgaggttg ctacgggcgg 6900
gctgctctgc tcggaagact atctgcctga agatggcatg tgagttggat gatatggttg 6960
gacgctggaa gacgttgaag ctggcgtctg tgagacctac cgcgtcacgc acgaaggagg 7020
cgtaggagtc gcgcagcttg ttgaccagct cggcggtgac ctgcacgtct agggcgcagt 7080
agtccagggt ttccttgatg atgtcatact tatcctgtcc cttttttttc cacagctcgc 7140
ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct 7200
ccgaacggta agagcctagc atgtagaact ggttgacggc ctggtaggcg cagcatccct 7260
tttctacggg tagcgcgtat gcctgcgcgg ccttccggag cgaggtgtgg gtgagcgcaa 7320
aggtgtccct gaccatgact ttgaggtact ggtatttgaa gtcagtgtcg tcgcatccgc 7380
cctgctccca gagcaaaaag tccgtgcgct ttttggaacg cggatttggc agggcgaagg 7440
tgacatcgtt gaagagtatc tttcccgcgc gaggcataaa gttgcgtgtg atgcggaagg 7500
gtcccggcac ctcggaacgg ttgttaatta cctgggcggc gagcacgatc tcgtcaaagc 7560
cgttgatgtt gtggcccaca atgtaaagtt ccaagaagcg cgggatgccc ttgatggaag 7620
gcaatttttt aagttcctcg taggtgagct cttcagggga gctgagcccg tgctctgaaa 7680
gggcccagtc tgcaagatga gggttggaag cgacgaatga gctccacagg tcacgggcca 7740
ttagcatttg caggtggtcg cgaaaggtcc taaactggcg acctatggcc attttttctg 7800
gggtgatgca gtagaaggta agcgggtctt gttcccagcg gtcccatcca aggttcgcgg 7860
ctaggtctcg cgcggcagtc actagaggct catctccgcc gaacttcatg accagcatga 7920
agggcacgag ctgcttccca aaggccccca tccaagtata ggtctctaca tcgtaggtga 7980
caaagagacg ctcggtgcga ggatgcgagc cgatcgggaa gaactggatc tcccgccacc 8040
aattggagga gtggctattg atgtggtgaa agtagaagtc cctgcgacgg gccgaacact 8100
cgtgctggct tttgtaaaaa cgtgcgcagt actggcagcg gtgcacgggc tgtacatcct 8160
gcacgaggtt gacctgacga ccgcgcacaa ggaagcagag tgggaatttg agcccctcgc 8220
ctggcgggtt tggctggtgg tcttctactt cggctgcttg tccttgaccg tctggctgct 8280
cgaggggagt tacggtggat cggaccacca cgccgcgcga gcccaaagtc cagatgtccg 8340
cgcgcggcgg tcggagcttg atgacaacat cgcgcagatg ggagctgtcc atggtctgga 8400
gctcccgcgg cgtcaggtca ggcgggagct cctgcaggtt tacctcgcat agacgggtca 8460
gggcgcgggc tagatccagg tgatacctaa tttccagggg ctggttggtg gcggcgtcga 8520
tggcttgcaa gaggccgcat ccccgcggcg cgactacggt accgcgcggc gggcggtggg 8580
ccgcgggggt gtccttggat gatgcatcta aaagcggtga cgcgggcgag cccccggagg 8640
tagggggggc tccggacccg ccgggagagg gggcaggggc acgtcggcgc cgcgcgcggg 8700
caggagctgg tgctgcgcgc gtaggttgct ggcgaacgcg acgacgcggc ggttgatctc 8760
ctgaatctgg cgcctctgcg tgaagacgac gggcccggtg agcttgagcc tgaaagagag 8820
ttcgacagaa tcaatttcgg tgtcgttgac ggcggcctgg cgcaaaatct cctgcacgtc 8880
tcctgagttg tcttgatagg cgatctcggc catgaactgc tcgatctctt cctcctggag 8940
atctccgcgt ccggctcgct ccacggtggc ggcgaggtcg ttggaaatgc gggccatgag 9000
ctgcgagaag gcgttgaggc ctccctcgtt ccagacgcgg ctgtagacca cgcccccttc 9060
ggcatcgcgg gcgcgcatga ccacctgcgc gagattgagc tccacgtgcc gggcgaagac 9120
ggcgtagttt cgcaggcgct gaaagaggta gttgagggtg gtggcggtgt gttctgccac 9180
gaagaagtac ataacccagc gtcgcaacgt ggattcgttg atatccccca aggcctcaag 9240
gcgctccatg gcctcgtaga agtccacggc gaagttgaaa aactgggagt tgcgcgccga 9300
cacggttaac tcctcctcca gaagacggat gagctcggcg acagtgtcgc gcacctcgcg 9360
ctcaaaggct acaggggcct cttcttcttc ttcaatctcc tcttccataa gggcctcccc 9420
ttcttcttct tctggcggcg gtgggggagg ggggacacgg cggcgacgac ggcgcaccgg 9480
gaggcggtcg acaaagcgct cgatcatctc cccgcggcga cggcgcatgg tctcggtgac 9540
ggcgcggccg ttctcgcggg ggcgcagttg gaagacgccg cccgtcatgt cccggttatg 9600
ggttggcggg gggctgccat gcggcaggga tacggcgcta acgatgcatc tcaacaattg 9660
ttgtgtaggt actccgccgc cgagggacct gagcgagtcc gcatcgaccg gatcggaaaa 9720
cctctcgaga aaggcgtcta accagtcaca gtcgcaaggt aggctgagca ccgtggcggg 9780
cggcagcggg cggcggtcgg ggttgtttct ggcggaggtg ctgctgatga tgtaattaaa 9840
gtaggcggtc ttgagacggc ggatggtcga cagaagcacc atgtccttgg gtccggcctg 9900
ctgaatgcgc aggcggtcgg ccatgcccca ggcttcgttt tgacatcggc gcaggtcttt 9960
gtagtagtct tgcatgagcc tttctaccgg cacttcttct tctccttcct cttgtcctgc 10020
atctcttgca tctatcgctg cggcggcggc ggagtttggc cgtaggtggc gccctcttcc 10080
tcccatgcgt gtgaccccga agcccctcat cggctgaagc agggctaggt cggcgacaac 10140
gcgctcggct aatatggcct gctgcacctg cgtgagggta gactggaagt catccatgtc 10200
cacaaagcgg tggtatgcgc ccgtgttgat ggtgtaagtg cagttggcca taacggacca 10260
gttaacggtc tggtgacccg gctgcgagag ctcggtgtac ctgagacgcg agtaagccct 10320
cgagtcaaat acgtagtcgt tgcaagtccg caccaggtac tggtatccca ccaaaaagtg 10380
cggcggcggc tggcggtaga ggggccagcg tagggtggcc ggggctccgg gggcgagatc 10440
ttccaacata aggcgatgat atccgtagat gtacctggac atccaggtga tgccggcggc 10500
ggtggtggag gcgcgcggaa agtcgcggac gcggttccag atgttgcgca gcggcaaaaa 10560
gtgctccatg gtcgggacgc tctggccggt caggcgcgcg caatcgttga cgctctagac 10620
cgtgcaaaag gagagcctgt aagcgggcac tcttccgtgg tctggtggat aaattcgcaa 10680
gggtatcatg gcggacgacc ggggttcgag ccccgtatcc ggccgtccgc cgtgatccat 10740
gcggttaccg cccgcgtgtc gaacccaggt gtgcgacgtc agacaacggg ggagtgctcc 10800
ttttggcttc cttccaggcg cggcggctgc tgcgctagct tttttggcca ctggccgcgc 10860
gcagcgtaag cggttaggct ggaaagcgaa agcattaagt ggctcgctcc ctgtagccgg 10920
agggttattt tccaagggtt gagtcgcggg acccccggtt cgagtctcgg accggccgga 10980
ctgcggcgaa cgggggtttg cctccccgtc atgcaagacc ccgcttgcaa attcctccgg 11040
aaacagggac gagccccttt tttgcttttc ccagatgcat ccggtgctgc ggcagatgcg 11100
cccccctcct cagcagcggc aagagcaaga gcagcggcag acatgcaggg caccctcccc 11160
tcctcctacc gcgtcaggag gggcgacatc cgcggttgac gcggcagcag atggtgatta 11220
cgaacccccg cggcgccggg cccggcacta cctggacttg gaggagggcg agggcctggc 11280
gcggctagga gcgccctctc ctgagcggca cccaagggtg cagctgaagc gtgatacgcg 11340
tgaggcgtac gtgccgcggc agaacctgtt tcgcgaccgc gagggagagg agcccgagga 11400
gatgcgggat cgaaagttcc acgcagggcg cgagctgcgg catggcctga atcgcgagcg 11460
gttgctgcgc gaggaggact ttgagcccga cgcgcgaacc gggattagtc ccgcgcgcgc 11520
acacgtggcg gccgccgacc tggtaaccgc atacgagcag acggtgaacc aggagattaa 11580
ctttcaaaaa agctttaaca accacgtgcg tacgcttgtg gcgcgcgagg aggtggctat 11640
aggactgatg catctgtggg actttgtaag cgcgctggag caaaacccaa atagcaagcc 11700
gctcatggcg cagctgttcc ttatagtgca gcacagcagg gacaacgagg cattcaggga 11760
tgcgctgcta aacatagtag agcccgaggg ccgctggctg ctcgatttga taaacatcct 11820
gcagagcata gtggtgcagg agcgcagctt gagcctggct gacaaggtgg ccgccatcaa 11880
ctattccatg cttagcctgg gcaagtttta cgcccgcaag atataccata ccccttacgt 11940
tcccatagac aaggaggtaa agatcgaggg gttctacatg cgcatggcgc tgaaggtgct 12000
taccttgagc gacgacctgg gcgtttatcg caacgagcgc atccacaagg ccgtgagcgt 12060
gagccggcgg cgcgagctca gcgaccgcga gctgatgcac agcctgcaaa gggccctggc 12120
tggcacgggc agcggcgata gagaggccga gtcctacttt gacgcgggcg ctgacctgcg 12180
ctgggcccca agccgacgcg ccctggaggc agctggggcc ggacctgggc tggcggtggc 12240
acccgcgcgc gctggcaacg tcggcggcgt ggaggaatat gacgaggacg atgagtacga 12300
gccagaggac ggcgagtact aagcggtgat gtttctgatc agatgatgca agacgcaacg 12360
gacccggcgg tgcgggcggc gctgcagagc cagccgtccg gccttaactc cacggacgac 12420
tggcgccagg tcatggaccg catcatgtcg ctgactgcgc gcaatcctga cgcgttccgg 12480
cagcagccgc aggccaaccg gctctccgca attctggaag cggtggtccc ggcgcgcgca 12540
aaccccacgc acgagaaggt gctggcgatc gtaaacgcgc tggccgaaaa cagggccatc 12600
cggcccgacg aggccggcct ggtctacgac gcgctgcttc agcgcgtggc tcgttacaac 12660
agcggcaacg tgcagaccaa cctggaccgg ctggtggggg atgtgcgcga ggccgtggcg 12720
cagcgtgagc gcgcgcagca gcagggcaac ctgggctcca tggttgcact aaacgccttc 12780
ctgagtacac agcccgccaa cgtgccgcgg ggacaggagg actacaccaa ctttgtgagc 12840
gcactgcggc taatggtgac tgagacaccg caaagtgagg tgtaccagtc tgggccagac 12900
tattttttcc agaccagtag acaaggcctg cagaccgtaa acctgagcca ggctttcaaa 12960
aacttgcagg ggctgtgggg ggtgcgggct cccacaggcg accgcgcgac cgtgtctagc 13020
ttgctgacgc ccaactcgcg cctgttgctg ctgctaatag cgcccttcac ggacagtggc 13080
agcgtgtccc gggacacata cctaggtcac ttgctgacac tgtaccgcga ggccataggt 13140
caggcgcatg tggacgagca tactttccag gagattacaa gtgtcagccg cgcgctgggg 13200
caggaggaca cgggcagcct ggaggcaacc ctaaactacc tgctgaccaa ccggcggcag 13260
aagatcccct cgttgcacag tttaaacagc gaggaggagc gcattttgcg ctacgtgcag 13320
cagagcgtga gccttaacct gatgcgcgac ggggtaacgc ccagcgtggc gctggacatg 13380
accgcgcgca acatggaacc gggcatgtat gcctcaaacc ggccgtttat caaccgccta 13440
atggactact tgcatcgcgc ggccgccgtg aaccccgagt atttcaccaa tgccatcttg 13500
aacccgcact ggctaccgcc ccctggtttc tacaccgggg gattcgaggt gcccgagggt 13560
aacgatggat tcctctggga cgacatagac gacagcgtgt tttccccgca accgcagacc 13620
ctgctagagt tgcaacagcg cgagcaggca gaggcggcgc tgcgaaagga aagcttccgc 13680
aggccaagca gcttgtccga tctaggcgct gcggccccgc ggtcagatgc tagtagccca 13740
tttccaagct tgatagggtc tcttaccagc actcgcacca cccgcccgcg cctgctgggc 13800
gaggaggagt acctaaacaa ctcgctgctg cagccgcagc gcgaaaaaaa cctgcctccg 13860
gcatttccca acaacgggat agagagccta gtggacaaga tgagtagatg gaagacgtac 13920
gcgcaggagc acagggacgt gccaggcccg cgcccgccca cccgtcgtca aaggcacgac 13980
cgtcagcggg gtctggtgtg ggaggacgat gactcggcag acgacagcag cgtcctggat 14040
ttgggaggga gtggcaaccc gtttgcgcac cttcgcccca ggctggggag aatgttttaa 14100
aaaaaaaaaa aaagcatgat gcaaaataaa aaactcacca aggccattgg gccaggattc 14160
tcctcgacgt caccagcctg cttcagcagg ctgaagttag tagctccgct tcccttatag 14220
agctcgttca tgccctcggt gatgccggcg gcggtcagga actccagcag caccatgtgg 14280
tcccgcttct cgttggggtc cttgaacagg gcgctctggt agctcaggta gtggttgtcg 14340
ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggccagctgc 14400
acgccgccgt cctcgatgtt gtgccggatc ttgaagttgg ccttgatgcc gttcttctgc 14460
ttgtcggcgg tgatgtacac gttgtggctg ttgtagttgt actccagctt gtggcccagg 14520
atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tccggttcac cagggtgtcg 14580
ccctcgaact tcacctcggc ccgggtcttg tagttgccgt cgtccttgaa gaagatggtc 14640
cgctcctgca cgtagccctc gggcatggcg ctcttgaaga agtcgtgctg cttcatgtgg 14700
tcggggtacc gggcgaagca ctgcacgccg tagcccaggg tggtcaccag ggtgggccag 14760
ggcacgggca gcttgccggt ggtgcacagc agcttcaggg tcagcttgcc gtaggtggcg 14820
tcgccctcgc cctcgccgct cacgctgaac ttgtggccgt tcacgtcgcc gtccagctcc 14880
accaggatgg gcaccacgcc ggtgaacagc tcttcgcctt tgctcaccat ggcaccgagc 14940
gttggttttc ttgtattccc cttagtatgc ggcgcgcggc gatgtatgag gaaggtcctc 15000
ctccctccta cgagagtgtg gtgagcgcgg cgccagtggc ggcggcgctg ggttctccct 15060
tcgatgctcc cctggacccg ccgtttgtgc ctccgcggta cctgcggcct accgggggga 15120
gaaacagcat ccgttactct gagttggcac ccctattcga caccacccgt gtgtacctgg 15180
tggacaacaa gtcaacggat gtggcatccc tgaactacca gaacgaccac agcaactttc 15240
tgaccacggt cattcaaaac aatgactaca gcccggggga ggcaagcaca cagaccatca 15300
atcttgacga ccggtcgcac tggggcggcg acctgaaaac catcctgcat accaacatgc 15360
caaatgtgaa cgagttcatg tttaccaata agtttaaggc gcgggtgatg gtgtcgcgct 15420
tgcctactaa ggacaatcag gtggagctga aatacgagtg ggtggagttc acgctgcccg 15480
agggcaacta ctccgagacc atgaccatag accttatgaa caacgcgatc gtggagcact 15540
acttgaaagt gggcagacag aacggggttc tggaaagcga catcggggta aagtttgaca 15600
cccgcaactt cagactgggg tttgaccccg tcactggtct tgtcatgcct ggggtatata 15660
caaacgaagc cttccatcca gacatcattt tgctgccagg atgcggggtg gacttcaccc 15720
acagccgcct gagcaacttg ttgggcatcc gcaagcggca acccttccag gagggcttta 15780
ggatcaccta cgatgatctg gagggtggta acattcccgc actgttggat gtggacgcct 15840
accaggcgag cttgaaagat gacaccgaac agggcggggg tggcgcaggc ggcagcaaca 15900
gcagtggcag cggcgcggaa gagaactcca acgcggcagc cgcggcaatg cagccggtgg 15960
aggacatgaa cgatcatgcc attcgcggcg acacctttgc cacacgggct gaggagaagc 16020
gcgctgaggc cgaagcagcg gccgaagctg ccgcccccgc tgcgcaaccc gaggtcgaga 16080
agcctcagaa gaaaccggtg atcaaacccc tgacagagga cagcaagaaa cgcagttaca 16140
acctaataag caatgacagc accttcaccc agtaccgcag ctggtacctt gcatacaact 16200
acggcgaccc tcagaccgga atccgctcat ggaccctgct ttgcactcct gacgtaacct 16260
gcggctcgga gcaggtctac tggtcgttgc cagacatgat gcaagacccc gtgaccttcc 16320
gctccacgcg ccagatcagc aactttccgg tggtgggcgc cgagctgttg cccgtgcact 16380
ccaagagctt ctacaacgac caggccgtct actcccaact catccgccag tttacctctc 16440
tgacccacgt gttcaatcgc tttcccgaga accagatttt ggcgcgcccg ccagccccca 16500
ccatcaccac cgtcagtgaa aacgttcctg ctctcacaga tcacgggacg ctaccgctgc 16560
gcaacagcat cggaggagtc cagcgagtga ccattactga cgccagacgc cgcacctgcc 16620
cctacgttta caaggccctg ggcatagtct cgccgcgcgt cctatcgagc cgcacttttt 16680
gagcaagcat gtccatcctt atatcgccca gcaataacac aggctggggc ctgcgcttcc 16740
caagcaagat gtttggcggg gccaagaagc gctccgacca acacccagtg cgcgtgcgcg 16800
ggcactaccg cgcgccctgg ggcgcgcaca aacgcggccg cactgggcgc accaccgtcg 16860
atgacgccat cgacgcggtg gtggaggagg cgcgcaacta cacgcccacg ccgccaccag 16920
tgtccacagt ggacgcggcc attcagaccg tggtgcgcgg agcccggcgc tatgctaaaa 16980
tgaagagacg gcggaggcgc gtagcacgtc gccaccgccg ccgacccggc actgccgccc 17040
aacgcgcggc ggcggccctg cttaaccgcg cacgtcgcac cggccgacgg gcggccatgc 17100
gggccgctcg aaggctggcc gcgggtattg tcactgtgcc ccccaggtcc aggcgacgag 17160
cggccgccgc agcagccgcg gccattagtg ctatgactca gggtcgcagg ggcaacgtgt 17220
attgggtgcg cgactcggtt agcggcctgc gcgtgcccgt gcgcacccgc cccccgcgca 17280
actagattgc aagaaaaaac tacttagact cgtactgttg tatgtatcca gcggcggcgg 17340
cgcgcaacga agctatgtcc aagcgcaaaa tcaaagaaga gatgctccag gtcatcgcgc 17400
cggagatcta tggccccccg aagaaggaag agcaggatta caagccccga aagctaaagc 17460
gggtcaaaaa gaaaaagaaa gatgatgatg atgaacttga cgacgaggtg gaactgctgc 17520
acgctaccgc gcccaggcga cgggtacagt ggaaaggtcg acgcgtaaaa cgtgttttgc 17580
gacccggcac caccgtagtc tttacgcccg gtgagcgctc cacccgcacc tacaagcgcg 17640
tgtatgatga ggtgtacggc gacgaggacc tgcttgagca ggccaacgag cgcctcgggg 17700
agtttgccta cggaaagcgg cataaggaca tgctggcgtt gccgctggac gagggcaacc 17760
caacacctag cctaaagccc gtaacactgc agcaggtgct gcccgcgctt gcaccgtccg 17820
aagaaaagcg cggcctaaag cgcgagtctg gtgacttggc acccaccgtg cagctgatgg 17880
tacccaagcg ccagcgactg gaagatgtct tggaaaaaat gaccgtggaa cctgggctgg 17940
agcccgaggt ccgcgtgcgg ccaatcaagc aggtggcgcc gggactgggc gtgcagaccg 18000
tggacgttca gatacccact accagtagca ccagtattgc caccgccaca gagggcatgg 18060
agacacaaac gtccccggtt gcctcagcgg tggcggatgc cgcggtgcag gcggtcgctg 18120
cggccgcgtc caagacctct acggaggtgc aaacggaccc gtggatgttt cgcgtttcag 18180
ccccccggcg cccgcgccgt tcgaggaagt acggcgccgc cagcgcgcta ctgcccgaat 18240
atgccctaca tccttccatt gcgcctaccc ccggctatcg tggctacacc taccgcccca 18300
gaagacgagc aactacccga cgccgaacca ccactggaac ccgccgccgc cgtcgccgtc 18360
gccagcccgt gctggccccg atttccgtgc gcagggtggc tcgcgaagga ggcaggaccc 18420
tggtgctgcc aacagcgcgc taccacccca gcatcgttta aaagccggtc tttgtggttc 18480
ttgcagatat ggccctcacc tgccgcctcc gtttcccggt gccgggattc cgaggaagaa 18540
tgcaccgtag gaggggcatg gccggccacg gcctgacggg cggcatgcgt cgtgcgcacc 18600
accggcggcg gcgcgcgtcg caccgtcgca tgcgcggcgg tatcctgccc ctccttattc 18660
cactgatcgc cgcggcgatt ggcgccgtgc ccggaattgc atccgtggcc ttgcaggcgc 18720
agagacactg attaaaaaca agttgcatgt ggaaaaatca aaataaaaag tctggactct 18780
cacgctcgct tggtcctgta actattttgt agaatggaag acatcaactt tgcgtctctg 18840
gccccgcgac acggctcgcg cccgttcatg ggaaactggc aagatatcgg caccagcaat 18900
atgagcggtg gcgccttcag ctggggctcg ctgtggagcg gcattaaaaa tttcggttcc 18960
accgttaaga actatggcag caaggcctgg aacagcagca caggccagat gctgagggat 19020
aagttgaaag agcaaaattt ccaacaaaag gtggtagatg gcctggcctc tggcattagc 19080
ggggtggtgg acctggccaa ccaggcagtg caaaataaga ttaacagtaa gcttgatccc 19140
cgccctcccg tagaggagcc tccaccggcc gtggagacag tgtctccaga ggggcgtggc 19200
gaaaagcgtc cgcgccccga cagggaagaa actctggtga cgcaaataga cgagcctccc 19260
tcgtacgagg aggcactaaa gcaaggcctg cccaccaccc gtcccatcgc gcccatggct 19320
accggagtgc tgggccagca cacacccgta acgctggacc tgcctccccc cgccgacacc 19380
cagcagaaac ctgtgctgcc aggcccgacc gccgttgttg taacccgtcc tagccgcgcg 19440
tccctgcgcc gcgccgccag cggtccgcga tcgttgcggc ccgtagccag tggcaactgg 19500
caaagcacac tgaacagcat cgtgggtctg ggggtgcaat ccctgaagcg ccgacgatgc 19560
ttctgaatag ctaacgtgtc gtatgtgtgt catgtatgcg tccatgtcgc cgccagagga 19620
gctgctgagc cgccgcgcgc ccgctttcca agatggctac cccttcgatg atgccgcagt 19680
ggtcttacat gcacatctcg ggccaggacg cctcggagta cctgagcccc gggctggtgc 19740
agtttgcccg cgccaccgag acgtacttca gcctgaataa caagtttaga aaccccacgg 19800
tggcgcctac gcacgacgtg accacagacc ggtcccagcg tttgacgctg cggttcatcc 19860
ctgtggaccg tgaggatact gcgtactcgt acaaggcgcg gttcacccta gctgtgggtg 19920
ataaccgtgt gctggacatg gcttccacgt actttgacat ccgcggcgtg ctggacaggg 19980
gccctacttt taagccctac tctggcactg cctacaacgc cctggctccc aagggtgccc 20040
caaatccttg cgaatgggat gaagctgcta ctgctcttga aataaaccta gaagaagagg 20100
acgatgacaa cgaagacgaa gtagacgagc aagctgagca gcaaaaaact cacgtatttg 20160
ggcaggcgcc ttattctggt ataaatatta caaaggaggg tattcaaata ggtgtcgaag 20220
gtcaaacacc taaatatgcc gataaaacat ttcaacctga acctcaaata ggagaatctc 20280
agtggtacga aactgaaatt aatcatgcag ctgggagagt ccttaaaaag actaccccaa 20340
tgaaaccatg ttacggttca tatgcaaaac ccacaaatga aaatggaggg caaggcattc 20400
ttgtaaagca acaaaatgga aagctagaaa gtcaagtgga aatgcaattt ttctcaacta 20460
ctgaggcgac cgcaggcaat ggtgataact tgactcctaa agtggtattg tacagtgaag 20520
atgtagatat agaaacccca gacactcata tttcttacat gcccactatt aaggaaggta 20580
actcacgaga actaatgggc caacaatcta tgcccaacag gcctaattac attgctttta 20640
gggacaattt tattggtcta atgtattaca acagcacggg taatatgggt gttctggcgg 20700
gccaagcatc gcagttgaat gctgttgtag atttgcaaga cagaaacaca gagctttcat 20760
accagctttt gcttgattcc attggtgata gaaccaggta cttttctatg tggaatcagg 20820
ctgttgacag ctatgatcca gatgttagaa ttattgaaaa tcatggaact gaagatgaac 20880
ttccaaatta ctgctttcca ctgggaggtg tgattaatac agagactctt accaaggtaa 20940
aacctaaaac aggtcaggaa aatggatggg aaaaagatgc tacagaattt tcagataaaa 21000
atgaaataag agttggaaat aattttgcca tggaaatcaa tctaaatgcc aacctgtgga 21060
gaaatttcct gtactccaac atagcgctgt atttgcccga caagctaaag tacagtcctt 21120
ccaacgtaaa aatttctgat aacccaaaca cctacgacta catgaacaag cgagtggtgg 21180
ctcccgggtt agtggactgc tacattaacc ttggagcacg ctggtccctt gactatatgg 21240
acaacgtcaa cccatttaac caccaccgca atgctggcct gcgctaccgc tcaatgttgc 21300
tgggcaatgg tcgctatgtg cccttccaca tccaggtgcc tcagaagttc tttgccatta 21360
aaaacctcct tctcctgccg ggctcataca cctacgagtg gaacttcagg aaggatgtta 21420
acatggttct gcagagctcc ctaggaaatg acctaagggt tgacggagcc agcattaagt 21480
ttgatagcat ttgcctttac gccaccttct tccccatggc ccacaacacc gcctccacgc 21540
ttgaggccat gcttagaaac gacaccaacg accagtcctt taacgactat ctctccgccg 21600
ccaacatgct ctaccctata cccgccaacg ctaccaacgt gcccatatcc atcccctccc 21660
gcaactgggc ggctttccgc ggctgggcct tcacgcgcct taagactaag gaaaccccat 21720
cactgggctc gggctacgac ccttattaca cctactctgg ctctataccc tacctagatg 21780
gaacctttta cctcaaccac acctttaaga aggtggccat tacctttgac tcttctgtca 21840
gctggcctgg caatgaccgc ctgcttaccc ccaacgagtt tgaaattaag cgctcagttg 21900
acggggaggg ttacaacgtt gcccagtgta acatgaccaa agactggttc ctggtacaaa 21960
tgctagctaa ctacaacatt ggctaccagg gcttctatat cccagagagc tacaaggacc 22020
gcatgtactc cttctttaga aacttccagc ccatgagccg tcaggtggtg gatgatacta 22080
aatacaagga ctaccaacag gtgggcatcc tacaccaaca caacaactct ggatttgttg 22140
gctaccttgc ccccaccatg cgcgaaggac aggcctaccc tgctaacttc ccctatccgc 22200
ttataggcaa gaccgcagtt gacagcatta cccagaaaaa gtttctttgc gatcgcaccc 22260
tttggcgcat cccattctcc agtaacttta tgtccatggg cgcactcaca gacctgggcc 22320
aaaaccttct ctacgccaac tccgcccacg cgctagacat gacttttgag gtggatccca 22380
tggacgagcc cacccttctt tatgttttgt ttgaagtctt tgacgtggtc cgtgtgcacc 22440
ggccgcaccg cggcgtcatc gaaaccgtgt acctgcgcac gcccttctcg gccggcaacg 22500
ccacaacata aagaagcaag caacatcaac aacagctgcc gccatgggct ccagtgagca 22560
ggaactgaaa gccattgtca aagatcttgg ttgtgggcca tattttttgg gcacctatga 22620
caagcgcttt ccaggctttg tttctccaca caagctcgcc tgcgccatag tcaatacggc 22680
cggtcgcgag actgggggcg tacactggat ggcctttgcc tggaacccgc actcaaaaac 22740
atgctacctc tttgagccct ttggcttttc tgaccagcga ctcaagcagg tttaccagtt 22800
tgagtacgag tcactcctgc gccgtagcgc cattgcttct tcccccgacc gctgtataac 22860
gctggaaaag tccacccaaa gcgtacaggg gcccaactcg gccgcctgtg gactattctg 22920
ctgcatgttt ctccacgcct ttgccaactg gccccaaact cccatggatc acaaccccac 22980
catgaacctt attaccgggg tacccaactc catgctcaac agtccccagg tacagcccac 23040
cctgcgtcgc aaccaggaac agctctacag cttcctggag cgccactcgc cctacttccg 23100
cagccacagt gcgcagatta ggagcgccac ttctttttgt cacttgaaaa acatgtaaaa 23160
ataatgtact agagacactt tcaataaagg caaatgcttt tatttgtaca ctctcgggtg 23220
attatttacc cccacccttg ccgtctgcgc cgtttaaaaa tcaaaggggt tctgccgcgc 23280
atcgctatgc gccactggca gggacacgtt gcgatactgg tgtttagtgc tccacttaaa 23340
ctcaggcaca accatccgcg gcagctcggt gaagttttca ctccacaggc tgcgcaccat 23400
caccaacgcg tttagcaggt cgggcgccga tatcttgaag tcgcagttgg ggcctccgcc 23460
ctgcgcgcgc gagttgcgat acacagggtt gcagcactgg aacactatca gcgccgggtg 23520
gtgcacgctg gccagcacgc tcttgtcgga gatcagatcc gcgtccaggt cctccgcgtt 23580
gctcagggcg aacggagtca actttggtag ctgccttccc aaaaagggcg cgtgcccagg 23640
ctttgagttg cactcgcacc gtagtggcat caaaaggtga ccgtgcccgg tctgggcgtt 23700
aggatacagc gcctgcataa aagccttgat ctgcttaaaa gccacctgag cctttgcgcc 23760
ttcagagaag aacatgccgc aagacttgcc ggaaaactga ttggccggac aggccgcgtc 23820
gtgcacgcag caccttgcgt cggtgttgga gatctgcacc acatttcggc cccaccggtt 23880
cttcacgatc ttggccttgc tagactgctc cttcagcgcg cgctgcccgt tttcgctcgt 23940
cacatccatt tcaatcacgt gctccttatt tatcataatg cttccgtgta gacacttaag 24000
ctcgccttcg atctcagcgc agcggtgcag ccacaacgcg cagcccgtgg gctcgtgatg 24060
cttgtaggtc acctctgcaa acgactgcag gtacgcctgc aggaatcgcc ccatcatcgt 24120
cacaaaggtc ttgttgctgg tgaaggtcag ctgcaacccg cggtgctcct cgttcagcca 24180
ggtcttgcat acggccgcca gagcttccac ttggtcaggc agtagtttga agttcgcctt 24240
tagatcgtta tccacgtggt acttgtccat cagcgcgcgc gcagcctcca tgcccttctc 24300
ccacgcagac acgatcggca cactcagcgg gttcatcacc gtaatttcac tttccgcttc 24360
gctgggctct tcctcttcct cttgcgtccg cataccacgc gccactgggt cgtcttcatt 24420
cagccgccgc actgtgcgct tacctccttt gccatgcttg attagcaccg gtgggttgct 24480
gaaacccacc atttgtagcg ccacatcttc tctttcttcc tcgctgtcca cgattacctc 24540
tggtgatggc gggcgctcgg gcttgggaga agggcgcttc tttttcttct tgggcgcaat 24600
ggccaaatcc gccgccgagg tcgatggccg cgggctgggt gtgcgcggca ccagcgcgtc 24660
ttgtgatgag tcttcctcgt cctcggactc gatacgccgc ctcatccgct tttttggggg 24720
cgcccgggga ggcggcggcg acggggacgg ggacgacacg tcctccatgg ttgggggacg 24780
tcgcgccgca ccgcgtccgc gctcgggggt ggtttcgcgc tgctcctctt cccgactggc 24840
catttccttc tcctataggc agaaaaagat catggagtca gtcgagaaga aggacagcct 24900
aaccgccccc tctgagttcg ccaccaccgc ctccaccgat gccgccaacg cgcctaccac 24960
cttccccgtc gaggcacccc cgcttgagga ggaggaagtg attatcgagc aggacccagg 25020
ttttgtaagc gaagacgacg aggaccgctc agtaccaaca gaggataaaa agcaagacca 25080
ggacaacgca gaggcaaacg aggaacaagt cgggcggggg gacgaaaggc atggcgacta 25140
cctagatgtg ggagacgacg tgctgttgaa gcatctgcag cgccagtgcg ccattatctg 25200
cgacgcgttg caagagcgca gcgatgtgcc cctcgccata gcggatgtca gccttgccta 25260
cgaacgccac ctattctcac cgcgcgtacc ccccaaacgc caagaaaacg gcacatgcga 25320
gcccaacccg cgcctcaact tctaccccgt atttgccgtg ccagaggtgc ttgccaccta 25380
tcacatcttt ttccaaaact gcaagatacc cctatcctgc cgtgccaacc gcagccgagc 25440
ggacaagcag ctggccttgc ggcagggcgc tgtcatacct gatatcgcct cgctcaacga 25500
agtgccaaaa atctttgagg gtcttggacg cgacgagaag cgcgcggcaa acgctctgca 25560
acaggaaaac agcgaaaatg aaagtcactc tggagtgttg gtggaactcg agggtgacaa 25620
cgcgcgccta gccgtactaa aacgcagcat cgaggtcacc cactttgcct acccggcact 25680
taacctaccc cccaaggtca tgagcacagt catgagtgag ctgatcgtgc gccgtgcgca 25740
gcccctggag agggatgcaa atttgcaaga acaaacagag gagggcctac ccgcagttgg 25800
cgacgagcag ctagcgcgct ggcttcaaac gcgcgagcct gccgacttgg aggagcgacg 25860
caaactaatg atggccgcag tgctcgttac cgtggagctt gagtgcatgc agcggttctt 25920
tgctgacccg gagatgcagc gcaagctaga ggaaacattg cactacacct ttcgacaggg 25980
ctacgtacgc caggcctgca agatctccaa cgtggagctc tgcaacctgg tctcctacct 26040
tggaattttg cacgaaaacc gccttgggca aaacgtgctt cattccacgc tcaagggcga 26100
ggcgcgccgc gactacgtcc gcgactgcgt ttacttattt ctatgctaca cctggcagac 26160
ggccatgggc gtttggcagc agtgcttgga ggagtgcaac ctcaaggagc tgcagaaact 26220
gctaaagcaa aacttgaagg acctatggac ggccttcaac gagcgctccg tggccgcgca 26280
cctggcggac atcattttcc ccgaacgcct gcttaaaacc ctgcaacagg gtctgccaga 26340
cttcaccagt caaagcatgt tgcagaactt taggaacttt atcctagagc gctcaggaat 26400
cttgcccgcc acctgctgtg cacttcctag cgactttgtg cccattaagt accgcgaatg 26460
ccctccgccg ctttggggcc actgctacct tctgcagcta gccaactacc ttgcctacca 26520
ctctgacata atggaagacg tgagcggtga cggtctactg gagtgtcact gtcgctgcaa 26580
cctatgcacc ccgcaccgct ccctggtttg caattcgcag ctgcttaacg aaagtcaaat 26640
tatcggtacc tttgagctgc agggtccctc gcctgacgaa aagtccgcgg ctccggggtt 26700
gaaactcact ccggggctgt ggacgtcggc ttaccttcgc aaatttgtac ctgaggacta 26760
ccacgcccac gagattaggt tctacgaaga ccaatcccgc ccgccaaatg cggagcttac 26820
cgcctgcgtc attacccagg gccacattct tggccaattg caagccatca acaaagcccg 26880
ccaagagttt ctgctacgaa agggacgggg ggtttacttg gacccccagt ccggcgagga 26940
gctcaaccca atccccccgc cgccgcagcc ctatcagcag cagccgcggg cccttgcttc 27000
ccaggatggc acccaaaaag aagctgcagc tgccgccgcc acccacggac gaggaggaat 27060
actgggacag tcaggcagag gaggttttgg acgaggagga ggaggacatg atggaagact 27120
gggagagcct agacgaggaa gcttccgagg tcgaagaggt gtcagacgaa acaccgtcac 27180
cctcggtcgc attcccctcg ccggcgcccc agaaatcggc aaccggttcc agcatggcta 27240
caacctccgc tcctcaggcg ccgccggcac tgcccgttcg ccgacccaac cgtagatggg 27300
acaccactgg aaccagggcc ggtaagtcca agcagccgcc gccgttagcc caagagcaac 27360
aacagcgcca aggctaccgc tcatggcgcg ggcacaagaa cgccatagtt gcttgcttgc 27420
aagactgtgg gggcaacatc tccttcgccc gccgctttct tctctaccat cacggcgtgg 27480
ccttcccccg taacatcctg cattactacc gtcatctcta cagcccatac tgcaccggcg 27540
gcagcggcag cggcagcaac agcagcggcc acacagaagc aaaggcgacc ggatagcaag 27600
actctgacaa agcccaagaa atccacagcg gcggcagcag caggaggagg agcgctgcgt 27660
ctggcgccca acgaacccgt atcgacccgc gagcttagaa acaggatttt tcccactctg 27720
tatgctatat ttcaacagag caggggccaa gaacaagagc tgaaaataaa aaacaggtct 27780
ctgcgatccc tcacccgcag ctgcctgtat cacaaaagcg aagatcagct tcggcgcacg 27840
ctggaagacg cggaggctct cttcagtaaa tactgcgcgc tgactcttaa ggactagttt 27900
cgcgcccttt ctcaaattta agcgcgaaaa ctacgtcatc tccagcggcc acacccggcg 27960
ccagcacctg tcgtcagcgc catttcaact ttgtatacaa aagttgtgat gagcaaggaa 28020
attcccacgc cctacatgtg gagttaccag ccacaaatgg gacttgcggc tggagctgcc 28080
caagactact caacccgaat aaactacatg agcgcgggac cccacatgat atcccgggtc 28140
aacggaatac gcgcccaccg aaaccgaatt ctcctggaac aggcggctat taccaccaca 28200
cctcgtaata accttaatcc ccgtagttgg cccgctgccc tggtgtacca ggaaagtccc 28260
gctcccacca ctgtggtact tcccagagac gcccaggccg aagttcagat gactaactca 28320
ggggcgcagc ttgcgggcgg ctttcgtcac agggtgcggt cgcccgggca gggtataact 28380
cacctgacaa tcagagggcg aggtattcag ctcaacgacg agtcggtgag ctcctcgctt 28440
ggtctccgtc cggacgggac atttcagatc ggcggcgccg gccgctcttc attcacgcct 28500
cgtcaggcaa tcctaactct gcagacctcg tcctctgagc cgcgctctgg aggcattgga 28560
actctgcaat ttattgagga gtttgtgcca tcggtctact ttaacccctt ctcgggacct 28620
cccggccact atccggatca atttattcct aactttgacg cggtaaagga ctcggcggac 28680
ggctacgact gaatgttaag tggagaggca gagcaactgc gcctgaaaca cctggtccac 28740
tgtcgccgcc acaagtgctt tgcccgcgac tccggtgagt tttgctactt tgaattgccc 28800
gaggatcata tcgagggccc ggcgcacggc gtccggctta ccgcccaggg agagcttgcc 28860
cgtagcctga ttcgggagtt tacccagcgc cccctgctag ttgagcggga caggggaccc 28920
tgtgttctca ctgtgatttg caactgtcct aaccctggat tacatcaaga tctttgttgc 28980
catctctgtg ctgagtataa taaatacaga aattaaaata tactggggct cctatcgcca 29040
tcctgtaaac gccaccgtct tcacccgccc aagcaaacca aggcgaacct tacctggtac 29100
ttttaacatc tctccctctg tgatttacaa cagtttcaac ccagacggag tgagtctacg 29160
agagaacctc tccgagctca gctactccat cagaaaaaac accaccctcc ttacctgccg 29220
ggaacgtacg agtgcgtcac cggccgctgc accacaccta ccgcctgacc gtaaaccaga 29280
ctttttccgg acagacctca ataactctgt ttaccagaac aggaggtgag cttagaaaac 29340
ccttagggta ttaggccaaa ggcgcagcta ctgtggggtt tatgaacaat tcaagcaact 29400
ctacgggcta ttctaattca ggtttctcta gaatcggggt tggggttatt ctctgtcttg 29460
tgattctctt tattcttata ctaacgcttc tctgcctaag gctcgccgcc tgctgtgtgc 29520
acatttgcat ttattgtcag ctttttaaac gctggggtcg ccacccaaga tgattaggta 29580
cataatccta ggtttactca cccttgcgtc agcccacggt accacccaaa aggtggattt 29640
taaggagcca gcctgtaatg ttacattcgc agctgaagct aatgagtgca ccactcttat 29700
aaaatgcacc acagaacatg aaaagctgct tattcgccac aaaaacaaaa ttggcaagta 29760
tgctgtttat gctatttggc agccaggtga cactacagag tataatgtta cagttttcca 29820
gggtaaaagt cataaaactt ttatgtatac ttttccattt tatgaaatgt gcgacattac 29880
catgtacatg agcaaacagt ataagttgtg gcccccacaa aattgtgtgg aaaacactgg 29940
cactttctgc tgcactgcta tgctaattac agtgctcgct ttggtctgta ccctactcta 30000
tattaaatac aaaagcagac gcagctttat tgaggaaaag aaaatgcctt aatttactaa 30060
gttacaaagc taatgtcacc actaactgct ttactcgctg cttgcaaaac aaattcaaaa 30120
agttagcatt ataattagaa taggatttaa accccccggt catttcctgc tcaataccat 30180
tcccctgaac aattgactct atgtgggata tgctccagcg ctacaacctt gaagtcaggc 30240
ttcctggatg tcagcatctg actttggcca gcacctgtcc cgcggatttg ttccagtcca 30300
actacagcga cccaccctaa cagagatgac caacacaacc aacgcggccg ccgctaccgg 30360
acttacatct accacaaata caccccaagt ttctgccttt gtcaataact gggataactt 30420
gggcatgtgg tggttctcca tagcgcttat gtttgtatgc cttattatta tgtggctcat 30480
ctgctgccta aagcgcaaac gcgcccgacc acccatctat agtcccatca ttgtgctaca 30540
cccaaacaat gatggaatcc atagattgga cggactgaaa cacatgttct tttctcttac 30600
agtatgatta aatgagacat gattcctcga gtttttatat tactgaccct tgttgcgctt 30660
ttttgtgcgt gctccacatt ggctgcggtt tctcacatcg aagtagactg cattccagcc 30720
ttcacagtct atttgcttta cggatttgtc accctcacgc tcatctgcag cctcatcact 30780
gtggtcatcg cctttatcca gtgcattgac tgggtctgtg tgcgctttgc atatctcaga 30840
caccatcccc agtacaggga caggactata gctgagcttc ttagaattct ttaattatga 30900
aatttactgt gacttttctg ctgattattt gcaccctatc tgcgttttgt tccccgacct 30960
ccaagcctca aagacatata tcatgcagat tcactcgtat atggaatatt ccaagttgct 31020
acaatgaaaa aagcgatctt tccgaagcct ggttatatgc aatcatctct gttatggtgt 31080
tctgcagtac catcttagcc ctagctatat atccctacct tgacattggc tggaacgcaa 31140
tagatgccat gaaccaccca actttccccg cgcccgctat gcttccactg caacaagttg 31200
ttgccggcgg ctttgtccca gccaatcagc ctcgcccacc ttctcccacc cccactgaaa 31260
tcagctactt taatctaaca ggaggagatg actgacaccc tagatctaga aatggacgga 31320
attattacag agcagcgcct gctagaaaga cgcagggcag cggccgagca acagcgcatg 31380
aatcaagagc tccaagacat ggttaacttg caccagtgca aaaggggtat cttttgtctg 31440
gtaaagcagg ccaaagtcac ctacgacagt aataccaccg gacaccgcct tagctacaag 31500
ttgccaacca agcgtcagaa attggtggtc atggtgggag aaaagcccat taccataact 31560
cagcactcgg tagaaaccga aggctgcatt cactcacctt gtcaaggacc tgaggatctc 31620
tgcaccctta ttaagaccct gtgcggtctc aaagatctta ttccctttaa ctaataaaaa 31680
aaaataataa agcatcactt acttaaaatc agttagcaaa tttctgtcca gtttattcag 31740
cagcacctcc ttgccctcct cccagctctg gtattgcagc ttcctcctgg ctgcaaactt 31800
tctccacaat ctaaatggaa tgtcagtttc ctcctgttcc tgtccatccg cacccactat 31860
cttcatgttg ttgcagatga agcgcgcaag accgtctgaa gataccttca accccgtgta 31920
tccatatgac acggaaaccg gtcctccaac tgtgcctttt cttactcctc cctttgtatc 31980
ccccaatggg tttcaagaga gtccccctgg ggtactctct ttgcgcctat ccgaacctct 32040
agttacctcc aatggcatgc ttgcgctcaa aatgggcaac ggcctctctc tggacgaggc 32100
cggcaacctt acctcccaaa atgtaaccac tgtgagccca cctctcaaaa aaaccaagtc 32160
aaacataaac ctggaaatat ctgcacccct cacagttacc tcagaagccc taactgtggc 32220
tgccgccgca cctctaatgg tcgcgggcaa cacactcacc atgcaatcac aggccccgct 32280
aaccgtgcac gactccaaac ttagcattgc cacccaagga cccctcacag tgtcagaagg 32340
aaagctagcc ctgcaaacat caggccccct caccaccacc gatagcagta cccttactat 32400
cactgcctca ccccctctaa ctactgccac tggtagcttg ggcattgact tgaaagagcc 32460
catttataca caaaatggaa aactaggact aaagtacggg gctcctttgc atgtaacaga 32520
cgacctaaac actttgaccg tagcaactgg tccaggtgtg actattaata atacttcctt 32580
gcaaactaaa gttactggag ccttgggttt tgattcacaa ggcaatatgc aacttaatgt 32640
agcaggagga ctaaggattg attctcaaaa cagacgcctt atacttgatg ttagttatcc 32700
gtttgatgct caaaaccaac taaatctaag actaggacag ggccctcttt ttataaactc 32760
agcccacaac ttggatatta actacaacaa aggcctttac ttgtttacag cttcaaacaa 32820
ttccaaaaag cttgaggtta acctaagcac tgccaagggg ttgatgtttg acgctacagc 32880
catagccatt aatgcaggag atgggcttga atttggttca cctaatgcac caaacacaaa 32940
tcccctcaaa acaaaaattg gccatggcct agaatttgat tcaaacaagg ctatggttcc 33000
taaactagga actggcctta gttttgacag cacaggtgcc attacagtag gaaacaaaaa 33060
taatgataag ctaactttgt ggaccacacc agctccatct cctaactgta gactaaatgc 33120
agagaaagat gctaaactca ctttggtctt aacaaaatgt ggcagtcaaa tacttgctac 33180
agtttcagtt ttggctgtta aaggcagttt ggctccaata tctggaacag ttcaaagtgc 33240
tcatcttatt ataagatttg acgaaaatgg agtgctacta aacaattcct tcctggaccc 33300
agaatattgg aactttagaa atggagatct tactgaaggc acagcctata caaacgctgt 33360
tggatttatg cctaacctat cagcttatcc aaaatctcac ggtaaaactg ccaaaagtaa 33420
cattgtcagt caagtttact taaacggaga caaaactaaa cctgtaacac taaccattac 33480
actaaacggt acacaggaaa caggagacac aactccaagt gcatactcta tgtcattttc 33540
atgggactgg tctggccaca actacattaa tgaaatattt gccacatcct cttacacttt 33600
ttcatacatt gcccaagaat aaagaatcgt ttgtgttatg tttcaacgtg tttatttttc 33660
aattgcagaa aatttcgaat catttttcat tcagtagtat agccccacca ccacatagct 33720
tatacagatc accgtacctc aactttgtat aataaagttg taatcaaact cacagaaccc 33780
tagtattcaa cctgccacct ccctcccaac acacagagta cacagtcctt tctccccggc 33840
tggccttaaa aagcatcata tcatgggtaa cagacatatt cttaggtgtt atattccaca 33900
cggtttcctg tcgagccaaa cgctcatcag tgatattaat aaactccccg ggcagctcac 33960
ttaagttcat gtcgctgtcc agctgctgag ccacaggctg ctgtccaact tgcggttgct 34020
taacgggcgg cgaaggagaa gtccacgcct acatgggggt agagtcataa tcgtgcatca 34080
ggatagggcg gtggtgctgc agcagcgcgc gaataaactg ctgccgccgc cgctccgtcc 34140
tgcaggaata caacatggca gtggtctcct cagcgatgat tcgcaccgcc cgcagcataa 34200
ggcgccttgt cctccgggca cagcagcgca ccctgatctc acttaaatca gcacagtaac 34260
tgcagcacag caccacaata ttgttcaaaa tcccacagtg caaggcgctg tatccaaagc 34320
tcatggcggg gaccacagaa cccacgtggc catcatacca caagcgcagg tagattaagt 34380
ggcgacccct cataaacacg ctggacataa acattacctc ttttggcatg ttgtaattca 34440
ccacctcccg gtaccatata aacctctgat taaacatggc gccatccacc accatcctaa 34500
accagctggc caaaacctgc ccgccggcta tacactgcag ggaaccggga ctggaacaat 34560
gacagtggag agcccaggac tcgtaaccat ggatcatcat gctcgtcatg atatcaatgt 34620
tggcacaaca caggcacacg tgcatacact tcctcaggat tacaagctcc tcccgcgtta 34680
gaaccatatc ccagggaaca acccattcct gaatcagcgt aaatcccaca ctgcagggaa 34740
gacctcgcac gtaactcacg ttgtgcattg tcaaagtgtt acattcgggc agcagcggat 34800
gatcctccag tatggtagcg cgggtttctg tctcaaaagg aggtagacga tccctactgt 34860
acggagtgcg ccgagacaac cgagatcgtg ttggtcgtag tgtcatgcca aatggaacgc 34920
cggacgtagt catatttcct gaagcaaaac caggtgcggg cgtgacaaac agatctgcgt 34980
ctccggtctc gccgcttaga tcgctctgtg tagtagttgt agtatatcca ctctctcaaa 35040
gcatccaggc gccccctggc ttcgggttct atgtaaactc cttcatgcgc cgctgccctg 35100
ataacatcca ccaccgcaga ataagccaca cccagccaac ctacacattc gttctgcgag 35160
tcacacacgg gaggagcggg aagagctgga agaaccatgt tttttttttt attccaaaag 35220
attatccaaa acctcaaaat gaagatctat taagtgaacg cgctcccctc cggtggcgtg 35280
gtcaaactct acagccaaag aacagataat ggcatttgta agatgttgca caatggcttc 35340
caaaaggcaa acggccctca cgtccaagtg gacgtaaagg ctaaaccctt cagggtgaat 35400
ctcctctata aacattccag caccttcaac catgcccaaa taattctcat ctcgccacct 35460
tctcaatata tctctaagca aatcccgaat attaagtccg gccattgtaa aaatctgctc 35520
cagagcgccc tccaccttca gcctcaagca gcgaatcatg attgcaaaaa ttcaggttcc 35580
tcacagacct gtataagatt caaaagcgga acattaacaa aaataccgcg atcccgtagg 35640
tcccttcgca gggccagctg aacataatcg tgcaggtctg cacggaccag cgcggccact 35700
tccccgccag gaaccatgac aaaagaaccc acactgatta tgacacgcat actcggagct 35760
atgctaacca gcgtagcccc gatgtaagct tgttgcatgg gcggcgatat aaaatgcaag 35820
gtgctgctca aaaaatcagg caaagcctcg cgcaaaaaag aaagcacatc gtagtcatgc 35880
tcatgcagat aaaggcaggt aagctccgga accaccacag aaaaagacac catttttctc 35940
tcaaacatgt ctgcgggttt ctgcataaac acaaaataaa ataacaaaaa aacatttaaa 36000
cattagaagc ctgtcttaca acaggaaaaa caacccttat aagcataaga cggactacgg 36060
ccatgccggc gtgaccgtaa aaaaactggt caccgtgatt aaaaagcacc accgacagct 36120
cctcggtcat gtccggagtc ataatgtaag actcggtaaa cacatcaggt tgattcacat 36180
cggtcagtgc taaaaagcga ccgaaatagc ccgggggaat acatacccgc aggcgtagag 36240
acaacattac agcccccata ggaggtataa caaaattaat aggagagaaa aacacataaa 36300
cacctgaaaa accctcctgc ctaggcaaaa tagcaccctc ccgctccaga acaacataca 36360
gcgcttccac agcggcagcc ataacagtca gccttaccag taaaaaagaa aacctattaa 36420
aaaaacacca ctcgacacgg caccagctca atcagtcaca gtgtaaaaaa gggccaagtg 36480
cagagcgagt atatatagga ctaaaaaatg acgtaacggt taaagtccac aaaaaacacc 36540
cagaaaaccg cacgcgaacc tacgcccaga aacgaaagcc aaaaaaccca caacttcctc 36600
aaatcgtcac ttccgttttc ccacgttacg tcacttccca ttttaagaaa actacaattc 36660
ccaacacata caagttactc cgccctaaaa cctacgtcac ccgccccgtt cccacgcccc 36720
gcgccacgtc acaaactcca ccccctcatt atcatattgg cttcaatcca aaataaggta 36780
tattattgat gatg 36794
<210> 6
<211> 36785
<212> DNA
<213> 人工序列
<220>
<223> 合成腺病毒基因组DBP-P2A-YPet
<400> 6
catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60
ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120
gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180
gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240
taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300
agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360
gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420
cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480
tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540
tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600
aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660
tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720
cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780
gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840
cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900
ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960
cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020
caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080
ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140
tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200
gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260
ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320
cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380
ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440
gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500
cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560
ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620
gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680
cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740
ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800
tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860
gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920
caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980
gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040
agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100
aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160
cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220
gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280
gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340
gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400
gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460
tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520
ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580
tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640
agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700
tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760
gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820
acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880
gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940
aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000
ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060
gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120
tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180
acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240
aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300
tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360
gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420
tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480
ttggctctag cgatgaagat acagattgag gtactgaaat gtgtgggcgt ggcttaaggg 3540
tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg 3600
ccgccgccca acttttctat acaaagttgt aatgagcacc aactcgtttg atggaagcat 3660
tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat 3720
gggctccagc attgatggtc gccccgtcct gcccgcaaac tctactacct tgacctacga 3780
gaccgtgtct ggaacgccgt tggagactgc agcctccgcc gccgcttcag ccgctgcagc 3840
caccgcccgc gggattgtga ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc 3900
ttcccgttca tccgcccgcg atgacaagtt gacggctctt ttggcacaat tggattcttt 3960
gacccgggaa cttaatgtcg tttctcagca gctgttggat ctgcgccagc aggtttctgc 4020
cctgaaggct tcctcccctc ccaatgcggt ttaaaacata aataaaaaac cagactctgt 4080
ttggatttgg atcaagcata agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg 4140
taggcccggg accagcggtc tcggtcgttg agggtcctgt gtattttttc caggacgtgg 4200
taaaggtgac tctggatgtt cagatacatg ggcataagcc cgtctctggg gtggaggtag 4260
caccactgca gagcttcatg ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag 4320
cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc tgattgccag gggcaggccc 4380
ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt gcatacgtgg ggatatgaga 4440
tgcatcttgg actgtatttt taggttggct atgttcccag ccatatccct ccggggattc 4500
atgttgtgca gaaccaccag cacagtgtat ccggtgcact tgggaaattt gtcatgtagc 4560
ttagaaggaa atgcgtggaa gaacttggag acgcccttgt gacctccaag attttccatg 4620
cattcgtcca taatgatggc aatgggccca cgggcggcgg cctgggcgaa gatatttctg 4680
ggatcactaa cgtcatagtt gtgttccagg atgagatcgt cataggccat ttttacaaag 4740
cgcgggcgga gggtgccaga ctgcggtata atggttccat ccggcccagg ggcgtagtta 4800
ccctcacaga tttgcatttc ccacgctttg agttcagatg gggggatcat gtctacctgc 4860
ggggcgatga agaaaacggt ttccggggta ggggagatca gctgggaaga aagcaggttc 4920
ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa tcacacctat taccgggtgc 4980
aactggtagt taagagagct gcagctgccg tcatccctga gcaggggggc cacttcgtta 5040
agcatgtccc tgactcgcat gttttccctg accaaatccg ccagaaggcg ctcgccgccc 5100
agcgatagca gttcttgcaa ggaagcaaag tttttcaacg gtttgagacc gtccgccgta 5160
ggcatgcttt tgagcgtttg accaagcagt tccaggcggt cccacagctc ggtcacctgc 5220
tctacggcat ctcgatccag catatctcct cgtttcgcgg gttggggcgg ctttcgctgt 5280
acggcagtag tcggtgctcg tccagacggg ccagggtcat gtctttccac gggcgcaggg 5340
tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc tccgggctgc gcgctggcca 5400
gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt 5460
cggccaggta gcatttgacc atggtgtcat agtccagccc ctccgcggcg tggcccttgg 5520
cgcgcagctt gcccttggag gaggcgccgc acgaggggca gtgcagactt ttgagggcgt 5580
agagcttggg cgcgagaaat accgattccg gggagtaggc atccgcgccg caggccccgc 5640
agacggtctc gcattccacg agccaggtga gctctggccg ttcggggtca aaaaccaggt 5700
ttcccccatg ctttttgatg cgtttcttac ctctggtttc catgagccgg tgtccacgct 5760
cggtgacgaa aaggctgtcc gtgtccccgt atacagactt gagaggcctg tcctcgagcg 5820
gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacaaag gctcgcgtcc 5880
aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact agggggtcca 5940
ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag gtgattggtt 6000
tgtaggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag ggggtggggg 6060
cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt tggggtgagt 6120
actccctctg aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg 6180
aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgca tccatctggt 6240
cagaaaagac aatctttttg ttgtcaagct tggtggcaaa cgacccgtag agggcgttgg 6300
acagcaactt ggcgatggag cgcagggttt ggtttttgtc gcgatcggcg cgctccttgg 6360
ccgcgatgtt tagctgcacg tattcgcgcg caacgcaccg ccattcggga aagacggtgg 6420
tgcgctcgtc gggcaccagg tgcacgcgcc aaccgcggtt gtgcagggtg acaaggtcaa 6480
cgctggtggc tacctctccg cgtaggcgct cgttggtcca gcagaggcgg ccgcccttgc 6540
gcgagcagaa tggcggtagg gggtctagct gcgtctcgtc cggggggtct gcgtccacgg 6600
taaagacccc gggcagcagg cgcgcgtcga agtagtctat cttgcatcct tgcaagtcta 6660
gcgcctgctg ccatgcgcgg gcggcaagcg cgcgctcgta tgggttgagt gggggacccc 6720
atggcatggg gtgggtgagc gcggaggcgt acatgccgca aatgtcgtaa acgtagaggg 6780
gctctctgag tattccaaga tatgtagggt agcatcttcc accgcggatg ctggcgcgca 6840
cgtaatcgta tagttcgtgc gagggagcga ggaggtcggg accgaggttg ctacgggcgg 6900
gctgctctgc tcggaagact atctgcctga agatggcatg tgagttggat gatatggttg 6960
gacgctggaa gacgttgaag ctggcgtctg tgagacctac cgcgtcacgc acgaaggagg 7020
cgtaggagtc gcgcagcttg ttgaccagct cggcggtgac ctgcacgtct agggcgcagt 7080
agtccagggt ttccttgatg atgtcatact tatcctgtcc cttttttttc cacagctcgc 7140
ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct 7200
ccgaacggta agagcctagc atgtagaact ggttgacggc ctggtaggcg cagcatccct 7260
tttctacggg tagcgcgtat gcctgcgcgg ccttccggag cgaggtgtgg gtgagcgcaa 7320
aggtgtccct gaccatgact ttgaggtact ggtatttgaa gtcagtgtcg tcgcatccgc 7380
cctgctccca gagcaaaaag tccgtgcgct ttttggaacg cggatttggc agggcgaagg 7440
tgacatcgtt gaagagtatc tttcccgcgc gaggcataaa gttgcgtgtg atgcggaagg 7500
gtcccggcac ctcggaacgg ttgttaatta cctgggcggc gagcacgatc tcgtcaaagc 7560
cgttgatgtt gtggcccaca atgtaaagtt ccaagaagcg cgggatgccc ttgatggaag 7620
gcaatttttt aagttcctcg taggtgagct cttcagggga gctgagcccg tgctctgaaa 7680
gggcccagtc tgcaagatga gggttggaag cgacgaatga gctccacagg tcacgggcca 7740
ttagcatttg caggtggtcg cgaaaggtcc taaactggcg acctatggcc attttttctg 7800
gggtgatgca gtagaaggta agcgggtctt gttcccagcg gtcccatcca aggttcgcgg 7860
ctaggtctcg cgcggcagtc actagaggct catctccgcc gaacttcatg accagcatga 7920
agggcacgag ctgcttccca aaggccccca tccaagtata ggtctctaca tcgtaggtga 7980
caaagagacg ctcggtgcga ggatgcgagc cgatcgggaa gaactggatc tcccgccacc 8040
aattggagga gtggctattg atgtggtgaa agtagaagtc cctgcgacgg gccgaacact 8100
cgtgctggct tttgtaaaaa cgtgcgcagt actggcagcg gtgcacgggc tgtacatcct 8160
gcacgaggtt gacctgacga ccgcgcacaa ggaagcagag tgggaatttg agcccctcgc 8220
ctggcgggtt tggctggtgg tcttctactt cggctgcttg tccttgaccg tctggctgct 8280
cgaggggagt tacggtggat cggaccacca cgccgcgcga gcccaaagtc cagatgtccg 8340
cgcgcggcgg tcggagcttg atgacaacat cgcgcagatg ggagctgtcc atggtctgga 8400
gctcccgcgg cgtcaggtca ggcgggagct cctgcaggtt tacctcgcat agacgggtca 8460
gggcgcgggc tagatccagg tgatacctaa tttccagggg ctggttggtg gcggcgtcga 8520
tggcttgcaa gaggccgcat ccccgcggcg cgactacggt accgcgcggc gggcggtggg 8580
ccgcgggggt gtccttggat gatgcatcta aaagcggtga cgcgggcgag cccccggagg 8640
tagggggggc tccggacccg ccgggagagg gggcaggggc acgtcggcgc cgcgcgcggg 8700
caggagctgg tgctgcgcgc gtaggttgct ggcgaacgcg acgacgcggc ggttgatctc 8760
ctgaatctgg cgcctctgcg tgaagacgac gggcccggtg agcttgagcc tgaaagagag 8820
ttcgacagaa tcaatttcgg tgtcgttgac ggcggcctgg cgcaaaatct cctgcacgtc 8880
tcctgagttg tcttgatagg cgatctcggc catgaactgc tcgatctctt cctcctggag 8940
atctccgcgt ccggctcgct ccacggtggc ggcgaggtcg ttggaaatgc gggccatgag 9000
ctgcgagaag gcgttgaggc ctccctcgtt ccagacgcgg ctgtagacca cgcccccttc 9060
ggcatcgcgg gcgcgcatga ccacctgcgc gagattgagc tccacgtgcc gggcgaagac 9120
ggcgtagttt cgcaggcgct gaaagaggta gttgagggtg gtggcggtgt gttctgccac 9180
gaagaagtac ataacccagc gtcgcaacgt ggattcgttg atatccccca aggcctcaag 9240
gcgctccatg gcctcgtaga agtccacggc gaagttgaaa aactgggagt tgcgcgccga 9300
cacggttaac tcctcctcca gaagacggat gagctcggcg acagtgtcgc gcacctcgcg 9360
ctcaaaggct acaggggcct cttcttcttc ttcaatctcc tcttccataa gggcctcccc 9420
ttcttcttct tctggcggcg gtgggggagg ggggacacgg cggcgacgac ggcgcaccgg 9480
gaggcggtcg acaaagcgct cgatcatctc cccgcggcga cggcgcatgg tctcggtgac 9540
ggcgcggccg ttctcgcggg ggcgcagttg gaagacgccg cccgtcatgt cccggttatg 9600
ggttggcggg gggctgccat gcggcaggga tacggcgcta acgatgcatc tcaacaattg 9660
ttgtgtaggt actccgccgc cgagggacct gagcgagtcc gcatcgaccg gatcggaaaa 9720
cctctcgaga aaggcgtcta accagtcaca gtcgcaaggt aggctgagca ccgtggcggg 9780
cggcagcggg cggcggtcgg ggttgtttct ggcggaggtg ctgctgatga tgtaattaaa 9840
gtaggcggtc ttgagacggc ggatggtcga cagaagcacc atgtccttgg gtccggcctg 9900
ctgaatgcgc aggcggtcgg ccatgcccca ggcttcgttt tgacatcggc gcaggtcttt 9960
gtagtagtct tgcatgagcc tttctaccgg cacttcttct tctccttcct cttgtcctgc 10020
atctcttgca tctatcgctg cggcggcggc ggagtttggc cgtaggtggc gccctcttcc 10080
tcccatgcgt gtgaccccga agcccctcat cggctgaagc agggctaggt cggcgacaac 10140
gcgctcggct aatatggcct gctgcacctg cgtgagggta gactggaagt catccatgtc 10200
cacaaagcgg tggtatgcgc ccgtgttgat ggtgtaagtg cagttggcca taacggacca 10260
gttaacggtc tggtgacccg gctgcgagag ctcggtgtac ctgagacgcg agtaagccct 10320
cgagtcaaat acgtagtcgt tgcaagtccg caccaggtac tggtatccca ccaaaaagtg 10380
cggcggcggc tggcggtaga ggggccagcg tagggtggcc ggggctccgg gggcgagatc 10440
ttccaacata aggcgatgat atccgtagat gtacctggac atccaggtga tgccggcggc 10500
ggtggtggag gcgcgcggaa agtcgcggac gcggttccag atgttgcgca gcggcaaaaa 10560
gtgctccatg gtcgggacgc tctggccggt caggcgcgcg caatcgttga cgctctagac 10620
cgtgcaaaag gagagcctgt aagcgggcac tcttccgtgg tctggtggat aaattcgcaa 10680
gggtatcatg gcggacgacc ggggttcgag ccccgtatcc ggccgtccgc cgtgatccat 10740
gcggttaccg cccgcgtgtc gaacccaggt gtgcgacgtc agacaacggg ggagtgctcc 10800
ttttggcttc cttccaggcg cggcggctgc tgcgctagct tttttggcca ctggccgcgc 10860
gcagcgtaag cggttaggct ggaaagcgaa agcattaagt ggctcgctcc ctgtagccgg 10920
agggttattt tccaagggtt gagtcgcggg acccccggtt cgagtctcgg accggccgga 10980
ctgcggcgaa cgggggtttg cctccccgtc atgcaagacc ccgcttgcaa attcctccgg 11040
aaacagggac gagccccttt tttgcttttc ccagatgcat ccggtgctgc ggcagatgcg 11100
cccccctcct cagcagcggc aagagcaaga gcagcggcag acatgcaggg caccctcccc 11160
tcctcctacc gcgtcaggag gggcgacatc cgcggttgac gcggcagcag atggtgatta 11220
cgaacccccg cggcgccggg cccggcacta cctggacttg gaggagggcg agggcctggc 11280
gcggctagga gcgccctctc ctgagcggta cccaagggtg cagctgaagc gtgatacgcg 11340
tgaggcgtac gtgccgcggc agaacctgtt tcgcgaccgc gagggagagg agcccgagga 11400
gatgcgggat cgaaagttcc acgcagggcg cgagctgcgg catggcctga atcgcgagcg 11460
gttgctgcgc gaggaggact ttgagcccga cgcgcgaacc gggattagtc ccgcgcgcgc 11520
acacgtggcg gccgccgacc tggtaaccgc atacgagcag acggtgaacc aggagattaa 11580
ctttcaaaaa agctttaaca accacgtgcg tacgcttgtg gcgcgcgagg aggtggctat 11640
aggactgatg catctgtggg actttgtaag cgcgctggag caaaacccaa atagcaagcc 11700
gctcatggcg cagctgttcc ttatagtgca gcacagcagg gacaacgagg cattcaggga 11760
tgcgctgcta aacatagtag agcccgaggg ccgctggctg ctcgatttga taaacatcct 11820
gcagagcata gtggtgcagg agcgcagctt gagcctggct gacaaggtgg ccgccatcaa 11880
ctattccatg cttagcctgg gcaagtttta cgcccgcaag atataccata ccccttacgt 11940
tcccatagac aaggaggtaa agatcgaggg gttctacatg cgcatggcgc tgaaggtgct 12000
taccttgagc gacgacctgg gcgtttatcg caacgagcgc atccacaagg ccgtgagcgt 12060
gagccggcgg cgcgagctca gcgaccgcga gctgatgcac agcctgcaaa gggccctggc 12120
tggcacgggc agcggcgata gagaggccga gtcctacttt gacgcgggcg ctgacctgcg 12180
ctgggcccca agccgacgcg ccctggaggc agctggggcc ggacctgggc tggcggtggc 12240
acccgcgcgc gctggcaacg tcggcggcgt ggaggaatat gacgaggacg atgagtacga 12300
gccagaggac ggcgagtact aagcggtgat gtttctgatc agatgatgca agacgcaacg 12360
gacccggcgg tgcgggcggc gctgcagagc cagccgtccg gccttaactc cacggacgac 12420
tggcgccagg tcatggaccg catcatgtcg ctgactgcgc gcaatcctga cgcgttccgg 12480
cagcagccgc aggccaaccg gctctccgca attctggaag cggtggtccc ggcgcgcgca 12540
aaccccacgc acgagaaggt gctggcgatc gtaaacgcgc tggccgaaaa cagggccatc 12600
cggcccgacg aggccggcct ggtctacgac gcgctgcttc agcgcgtggc tcgttacaac 12660
agcggcaacg tgcagaccaa cctggaccgg ctggtggggg atgtgcgcga ggccgtggcg 12720
cagcgtgagc gcgcgcagca gcagggcaac ctgggctcca tggttgcact aaacgccttc 12780
ctgagtacac agcccgccaa cgtgccgcgg ggacaggagg actacaccaa ctttgtgagc 12840
gcactgcggc taatggtgac tgagacaccg caaagtgagg tgtaccagtc tgggccagac 12900
tattttttcc agaccagtag acaaggcctg cagaccgtaa acctgagcca ggctttcaaa 12960
aacttgcagg ggctgtgggg ggtgcgggct cccacaggcg accgcgcgac cgtgtctagc 13020
ttgctgacgc ccaactcgcg cctgttgctg ctgctaatag cgcccttcac ggacagtggc 13080
agcgtgtccc gggacacata cctaggtcac ttgctgacac tgtaccgcga ggccataggt 13140
caggcgcatg tggacgagca tactttccag gagattacaa gtgtcagccg cgcgctgggg 13200
caggaggaca cgggcagcct ggaggcaacc ctaaactacc tgctgaccaa ccggcggcag 13260
aagatcccct cgttgcacag tttaaacagc gaggaggagc gcattttgcg ctacgtgcag 13320
cagagcgtga gccttaacct gatgcgcgac ggggtaacgc ccagcgtggc gctggacatg 13380
accgcgcgca acatggaacc gggcatgtat gcctcaaacc ggccgtttat caaccgccta 13440
atggactact tgcatcgcgc ggccgccgtg aaccccgagt atttcaccaa tgccatcttg 13500
aacccgcact ggctaccgcc ccctggtttc tacaccgggg gattcgaggt gcccgagggt 13560
aacgatggat tcctctggga cgacatagac gacagcgtgt tttccccgca accgcagacc 13620
ctgctagagt tgcaacagcg cgagcaggca gaggcggcgc tgcgaaagga aagcttccgc 13680
aggccaagca gcttgtccga tctaggcgct gcggccccgc ggtcagatgc tagtagccca 13740
tttccaagct tgatagggtc tcttaccagc actcgcacca cccgcccgcg cctgctgggc 13800
gaggaggagt acctaaacaa ctcgctgctg cagccgcagc gcgaaaaaaa cctgcctccg 13860
gcatttccca acaacgggat agagagccta gtggacaaga tgagtagatg gaagacgtac 13920
gcgcaggagc acagggacgt gccaggcccg cgcccgccca cccgtcgtca aaggcacgac 13980
cgtcagcggg gtctggtgtg ggaggacgat gactcggcag acgacagcag cgtcctggat 14040
ttgggaggga gtggcaaccc gtttgcgcac cttcgcccca ggctggggag aatgttttaa 14100
aaaaaaaaaa gcatgatgca aaataaaaaa ctcaccaagg ccatggcacc gagcgttggt 14160
tttcttgtat tccccttagt atgcggcgcg cggcgatgta tgaggaaggt cctcctccct 14220
cctacgagag tgtggtgagc gcggcgccag tggcggcggc gctgggttct cccttcgatg 14280
ctcccctgga cccgccgttt gtgcctccgc ggtacctgcg gcctaccggg gggagaaaca 14340
gcatccgtta ctctgagttg gcacccctat tcgacaccac ccgtgtgtac ctggtggaca 14400
acaagtcaac ggatgtggca tccctgaact accagaacga ccacagcaac tttctgacca 14460
cggtcattca aaacaatgac tacagcccgg gggaggcaag cacacagacc atcaatcttg 14520
acgaccggtc gcactggggc ggcgacctga aaaccatcct gcataccaac atgccaaatg 14580
tgaacgagtt catgtttacc aataagttta aggcgcgggt gatggtgtcg cgcttgccta 14640
ctaaggacaa tcaggtggag ctgaaatacg agtgggtgga gttcacgctg cccgagggca 14700
actactccga gaccatgacc atagacctta tgaacaacgc gatcgtggag cactacttga 14760
aagtgggcag acagaacggg gttctggaaa gcgacatcgg ggtaaagttt gacacccgca 14820
acttcagact ggggtttgac cccgtcactg gtcttgtcat gcctggggta tatacaaacg 14880
aagccttcca tccagacatc attttgctgc caggatgcgg ggtggacttc acccacagcc 14940
gcctgagcaa cttgttgggc atccgcaagc ggcaaccctt ccaggagggc tttaggatca 15000
cctacgatga tctggagggt ggtaacattc ccgcactgtt ggatgtggac gcctaccagg 15060
cgagcttgaa agatgacacc gaacagggcg ggggtggcgc aggcggcagc aacagcagtg 15120
gcagcggcgc ggaagagaac tccaacgcgg cagccgcggc aatgcagccg gtggaggaca 15180
tgaacgatca tgccattcgc ggcgacacct ttgccacacg ggctgaggag aagcgcgctg 15240
aggccgaagc agcggccgaa gctgccgccc ccgctgcgca acccgaggtc gagaagcctc 15300
agaagaaacc ggtgatcaaa cccctgacag aggacagcaa gaaacgcagt tacaacctaa 15360
taagcaatga cagcaccttc acccagtacc gcagctggta ccttgcatac aactacggcg 15420
accctcagac cggaatccgc tcatggaccc tgctttgcac tcctgacgta acctgcggct 15480
cggagcaggt ctactggtcg ttgccagaca tgatgcaaga ccccgtgacc ttccgctcca 15540
cgcgccagat cagcaacttt ccggtggtgg gcgccgagct gttgcccgtg cactccaaga 15600
gcttctacaa cgaccaggcc gtctactccc aactcatccg ccagtttacc tctctgaccc 15660
acgtgttcaa tcgctttccc gagaaccaga ttttggcgcg cccgccagcc cccaccatca 15720
ccaccgtcag tgaaaacgtt cctgctctca cagatcacgg gacgctaccg ctgcgcaaca 15780
gcatcggagg agtccagcga gtgaccatta ctgacgccag acgccgcacc tgcccctacg 15840
tttacaaggc cctgggcata gtctcgccgc gcgtcctatc gagccgcact ttttgagcaa 15900
gcatgtccat ccttatatcg cccagcaata acacaggctg gggcctgcgc ttcccaagca 15960
agatgtttgg cggggccaag aagcgctccg accaacaccc agtgcgcgtg cgcgggcact 16020
accgcgcgcc ctggggcgcg cacaaacgcg gccgcactgg gcgcaccacc gtcgatgacg 16080
ccatcgacgc ggtggtggag gaggcgcgca actacacgcc cacgccgcca ccagtgtcca 16140
cagtggacgc ggccattcag accgtggtgc gcggagcccg gcgctatgct aaaatgaaga 16200
gacggcggag gcgcgtagca cgtcgccacc gccgccgacc cggcactgcc gcccaacgcg 16260
cggcggcggc cctgcttaac cgcgcacgtc gcaccggccg acgggcggcc atgcgggccg 16320
ctcgaaggct ggccgcgggt attgtcactg tgccccccag gtccaggcga cgagcggccg 16380
ccgcagcagc cgcggccatt agtgctatga ctcagggtcg caggggcaac gtgtattggg 16440
tgcgcgactc ggttagcggc ctgcgcgtgc ccgtgcgcac ccgccccccg cgcaactaga 16500
ttgcaagaaa aaactactta gactcgtact gttgtatgta tccagcggcg gcggcgcgca 16560
acgaagctat gtccaagcgc aaaatcaaag aagagatgct ccaggtcatc gcgccggaga 16620
tctatggccc cccgaagaag gaagagcagg attacaagcc ccgaaagcta aagcgggtca 16680
aaaagaaaaa gaaagatgat gatgatgaac ttgacgacga ggtggaactg ctgcacgcta 16740
ccgcgcccag gcgacgggta cagtggaaag gtcgacgcgt aaaacgtgtt ttgcgacccg 16800
gcaccaccgt agtctttacg cccggtgagc gctccacccg cacctacaag cgcgtgtatg 16860
atgaggtgta cggcgacgag gacctgcttg agcaggccaa cgagcgcctc ggggagtttg 16920
cctacggaaa gcggcataag gacatgctgg cgttgccgct ggacgagggc aacccaacac 16980
ctagcctaaa gcccgtaaca ctgcagcagg tgctgcccgc gcttgcaccg tccgaagaaa 17040
agcgcggcct aaagcgcgag tctggtgact tggcacccac cgtgcagctg atggtaccca 17100
agcgccagcg actggaagat gtcttggaaa aaatgaccgt ggaacctggg ctggagcccg 17160
aggtccgcgt gcggccaatc aagcaggtgg cgccgggact gggcgtgcag accgtggacg 17220
ttcagatacc cactaccagt agcaccagta ttgccaccgc cacagagggc atggagacac 17280
aaacgtcccc ggttgcctca gcggtggcgg atgccgcggt gcaggcggtc gctgcggccg 17340
cgtccaagac ctctacggag gtgcaaacgg acccgtggat gtttcgcgtt tcagcccccc 17400
ggcgcccgcg cggttcgagg aagtacggcg ccgccagcgc gctactgccc gaatatgccc 17460
tacatccttc cattgcgcct acccccggct atcgtggcta cacctaccgc cccagaagac 17520
gagcaactac ccgacgccga accaccactg gaacccgccg ccgccgtcgc cgtcgccagc 17580
ccgtgctggc cccgatttcc gtgcgcaggg tggctcgcga aggaggcagg accctggtgc 17640
tgccaacagc gcgctaccac cccagcatcg tttaaaagcc ggtctttgtg gttcttgcag 17700
atatggccct cacctgccgc ctccgtttcc cggtgccggg attccgagga agaatgcacc 17760
gtaggagggg catggccggc cacggcctga cgggcggcat gcgtcgtgcg caccaccggc 17820
ggcggcgcgc gtcgcaccgt cgcatgcgcg gcggtatcct gcccctcctt attccactga 17880
tcgccgcggc gattggcgcc gtgcccggaa ttgcatccgt ggccttgcag gcgcagagac 17940
actgattaaa aacaagttgc atgtggaaaa atcaaaataa aaagtctgga ctctcacgct 18000
cgcttggtcc tgtaactatt ttgtagaatg gaagacatca actttgcgtc tctggccccg 18060
cgacacggct cgcgcccgtt catgggaaac tggcaagata tcggcaccag caatatgagc 18120
ggtggcgcct tcagctgggg ctcgctgtgg agcggcatta aaaatttcgg ttccaccgtt 18180
aagaactatg gcagcaaggc ctggaacagc agcacaggcc agatgctgag ggataagttg 18240
aaagagcaaa atttccaaca aaaggtggta gatggcctgg cctctggcat tagcggggtg 18300
gtggacctgg ccaaccaggc agtgcaaaat aagattaaca gtaagcttga tccccgccct 18360
cccgtagagg agcctccacc ggccgtggag acagtgtctc cagaggggcg tggcgaaaag 18420
cgtccgcgcc ccgacaggga agaaactctg gtgacgcaaa tagacgagcc tccctcgtac 18480
gaggaggcac taaagcaagg cctgcccacc acccgtccca tcgcgcccat ggctaccgga 18540
gtgctgggcc agcacacacc cgtaacgctg gacctgcctc cccccgccga cacccagcag 18600
aaacctgtgc tgccaggccc gaccgccgtt gttgtaaccc gtcctagccg cgcgtccctg 18660
cgccgcgccg ccagcggtcc gcgatcgttg cggcccgtag ccagtggcaa ctggcaaagc 18720
acactgaaca gcatcgtggg tctgggggtg caatccctga agcgccgacg atgcttctga 18780
atagctaacg tgtcgtatgt gtgtcatgta tgcgtccatg tcgccgccag aggagctgct 18840
gagccgccgc gcgcccgctt tccaagatgg ctaccccttc gatgatgccg cagtggtctt 18900
acatgcacat ctcgggccag gacgcctcgg agtacctgag ccccgggctg gtgcagtttg 18960
cccgcgccac cgagacgtac ttcagcctga ataacaagtt tagaaacccc acggtggcgc 19020
ctacgcacga cgtgaccaca gaccggtccc agcgtttgac gctgcggttc atccctgtgg 19080
accgtgagga tactgcgtac tcgtacaagg cgcggttcac cctagctgtg ggtgataacc 19140
gtgtgctgga catggcttcc acgtactttg acatccgcgg cgtgctggac aggggcccta 19200
cttttaagcc ctactctggc actgcctaca acgccctggc tcccaagggt gccccaaatc 19260
cttgcgaatg ggatgaagct gctactgctc ttgaaataaa cctagaagaa gaggacgatg 19320
acaacgaaga cgaagtagac gagcaagctg agcagcaaaa aactcacgta tttgggcagg 19380
cgccttattc tggtataaat attacaaagg agggtattca aataggtgtc gaaggtcaaa 19440
cacctaaata tgccgataaa acatttcaac ctgaacctca aataggagaa tctcagtggt 19500
acgaaactga aattaatcat gcagctggga gagtccttaa aaagactacc ccaatgaaac 19560
catgttacgg ttcatatgca aaacccacaa atgaaaatgg agggcaaggc attcttgtaa 19620
agcaacaaaa tggaaagcta gaaagtcaag tggaaatgca atttttctca actactgagg 19680
cgaccgcagg caatggtgat aacttgactc ctaaagtggt attgtacagt gaagatgtag 19740
atatagaaac cccagacact catatttctt acatgcccac tattaaggaa ggtaactcac 19800
gagaactaat gggccaacaa tctatgccca acaggcctaa ttacattgct tttagggaca 19860
attttattgg tctaatgtat tacaacagca cgggtaatat gggtgttctg gcgggccaag 19920
catcgcagtt gaatgctgtt gtagatttgc aagacagaaa cacagagctt tcataccagc 19980
ttttgcttga ttccattggt gatagaacca ggtacttttc tatgtggaat caggctgttg 20040
acagctatga tccagatgtt agaattattg aaaatcatgg aactgaagat gaacttccaa 20100
attactgctt tccactggga ggtgtgatta atacagagac tcttaccaag gtaaaaccta 20160
aaacaggtca ggaaaatgga tgggaaaaag atgctacaga attttcagat aaaaatgaaa 20220
taagagttgg aaataatttt gccatggaaa tcaatctaaa tgccaacctg tggagaaatt 20280
tcctgtactc caacatagcg ctgtatttgc ccgacaagct aaagtacagt ccttccaacg 20340
taaaaatttc tgataaccca aacacctacg actacatgaa caagcgagtg gtggctcccg 20400
ggttagtgga ctgctacatt aaccttggag cacgctggtc ccttgactat atggacaacg 20460
tcaacccatt taaccaccac cgcaatgctg gcctgcgcta ccgctcaatg ttgctgggca 20520
atggtcgcta tgtgcccttc cacatccagg tgcctcagaa gttctttgcc attaaaaacc 20580
tccttctcct gccgggctca tacacctacg agtggaactt caggaaggat gttaacatgg 20640
ttctgcagag ctccctagga aatgacctaa gggttgacgg agccagcatt aagtttgata 20700
gcatttgcct ttacgccacc ttcttcccca tggcccacaa caccgcctcc acgcttgagg 20760
ccatgcttag aaacgacacc aacgaccagt cctttaacga ctatctctcc gccgccaaca 20820
tgctctaccc tatacccgcc aacgctacca acgtgcccat atccatcccc tcccgcaact 20880
gggcggcttt ccgcggctgg gccttcacgc gccttaagac taaggaaacc ccatcactgg 20940
gctcgggcta cgacccttat tacacctact ctggctctat accctaccta gatggaacct 21000
tttacctcaa ccacaccttt aagaaggtgg ccattacctt tgactcttct gtcagctggc 21060
ctggcaatga ccgcctgctt acccccaacg agtttgaaat taagcgctca gttgacgggg 21120
agggttacaa cgttgcccag tgtaacatga ccaaagactg gttcctggta caaatgctag 21180
ctaactataa cattggctac cagggcttct atatcccaga gagctacaag gaccgcatgt 21240
actccttctt tagaaacttc cagcccatga gccgtcaggt ggtggatgat actaaataca 21300
aggactacca acaggtgggc atcctacacc aacacaacaa ctctggattt gttggctacc 21360
ttgcccccac catgcgcgaa ggacaggcct accctgctaa cttcccctat ccgcttatag 21420
gcaagaccgc agttgacagc attacccaga aaaagtttct ttgcgatcgc accctttggc 21480
gcatcccatt ctccagtaac tttatgtcca tgggcgcact cacagacctg ggccaaaacc 21540
ttctctacgc caactccgcc cacgcgctag acatgacttt tgaggtggat cccatggacg 21600
agcccaccct tctttatgtt ttgtttgaag tctttgacgt ggtccgtgtg caccagccgc 21660
accgcggcgt catcgaaacc gtgtacctgc gcacgccctt ctcggccggc aacgccacaa 21720
cataaagaag caagcaacat caacaacagc tgccgccatg ggctccagtg agcaggaact 21780
gaaagccatt gtcaaagatc ttggttgtgg gccatatttt ttgggcacct atgacaagcg 21840
ctttccaggc tttgtttctc cacacaagct cgcctgcgcc atagtcaata cggccggtcg 21900
cgagactggg ggcgtacact ggatggcctt tgcctggaac ccgcactcaa aaacatgcta 21960
cctctttgag ccctttggct tttctgacca gcgactcaag caggtttacc agtttgagta 22020
cgagtcactc ctgcgccgta gcgccattgc ttcttccccc gaccgctgta taacgctgga 22080
aaagtccacc caaagcgtac aggggcccaa ctcggccgcc tgtggactat tctgctgcat 22140
gtttctccac gcctttgcca actggcccca aactcccatg gatcacaacc ccaccatgaa 22200
ccttattacc ggggtaccca actccatgct caacagtccc caggtacagc ccaccctgcg 22260
tcgcaaccag gaacagctct acagcttcct ggagcgccac tcgccctact tccgcagcca 22320
cagtgcgcag attaggagcg ccacttcttt ttgtcacttg aaaaacatgt aaaaataatg 22380
tactagagac actttcaata aaggcaaatg cttttatttg tacactctcg ggtgattatt 22440
tacccccacc cttgccgtct gcgccgttta cttatagagc tcgttcatgc cctcggtgat 22500
gccggcggcg gtcaggaact ccagcagcac catgtggtcc cgcttctcgt tggggtcctt 22560
gaacagggcg ctctggtagc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc 22620
gatgggggtg ttctgctggt agtggtcggc cagctgcacg ccgccgtcct cgatgttgtg 22680
ccggatcttg aagttggcct tgatgccgtt cttctgcttg tcggcggtga tgtacacgtt 22740
gtggctgttg tagttgtact ccagcttgtg gcccaggatg ttgccgtcct ccttgaagtc 22800
gatgcccttc agctcgatcc ggttcaccag ggtgtcgccc tcgaacttca cctcggcccg 22860
ggtcttgtag ttgccgtcgt ccttgaagaa gatggtccgc tcctgcacgt agccctcggg 22920
catggcgctc ttgaagaagt cgtgctgctt catgtggtcg gggtaccggg cgaagcactg 22980
cacgccgtag cccagggtgg tcaccagggt gggccagggc acgggcagct tgccggtggt 23040
gcacagcagc ttcagggtca gcttgccgta ggtggcgtcg ccctcgccct cgccgctcac 23100
gctgaacttg tggccgttca cgtcgccgtc cagctccacc aggatgggca ccacgccggt 23160
gaacagctct tcgcctttgc tcaccattgg gccaggattc tcctcgacgt caccagcctg 23220
cttcagcagg ctgaagttag tagctccgct tccaaaatca aaggggttct gccgcgcatc 23280
gctatgcgcc actggcaggg acacgttgcg atactggtgt ttagtgctcc acttaaactc 23340
aggcacaacc atccgcggca gctcggtgaa gttttcactc cacaggctgc gcaccatcac 23400
caacgcgttt agcaggtcgg gcgccgatat cttgaagtcg cagttggggc ctccgccctg 23460
cgcgcgcgag ttgcgataca cagggttgca gcactggaac actatcagcg ccgggtggtg 23520
cacgctggcc agcacgctct tgtcggagat cagatccgcg tccaggtcct ccgcgttgct 23580
cagggcgaac ggagtcaact ttggtagctg ccttcccaaa aagggcgcgt gcccaggctt 23640
tgagttgcac tcgcaccgta gtggcatcaa aaggtgaccg tgcccggtct gggcgttagg 23700
atacagcgcc tgcataaaag ccttgatctg cttaaaagcc acctgagcct ttgcgccttc 23760
agagaagaac atgccgcaag acttgccgga aaactgattg gccggacagg ccgcgtcgtg 23820
cacgcagcac cttgcgtcgg tgttggagat ctgcaccaca tttcggcccc accggttctt 23880
cacgatcttg gccttgctag actgctcctt cagcgcgcgc tgcccgtttt cgctcgtcac 23940
atccatttca atcacgtgct ccttatttat cataatgctt ccgtgtagac acttaagctc 24000
gccttcgatc tcagcgcagc ggtgcagcca caacgcgcag cccgtgggct cgtgatgctt 24060
gtaggtcacc tctgcaaacg actgcaggta cgcctgcagg aatcgcccca tcatcgtcac 24120
aaaggtcttg ttgctggtga aggtcagctg caacccgcgg tgctcctcgt tcagccaggt 24180
cttgcatacg gccgccagag cttccacttg gtcaggcagt agtttgaagt tcgcctttag 24240
atcgttatcc acgtggtact tgtccatcag cgcgcgcgca gcctccatgc ccttctccca 24300
cgcagacacg atcggcacac tcagcgggtt catcaccgta atttcacttt ccgcttcgct 24360
gggctcttcc tcttcctctt gcgtccgcat accacgcgcc actgggtcgt cttcattcag 24420
ccgccgcact gtgcgcttac ctcctttgcc atgcttgatt agcaccggtg ggttgctgaa 24480
acccaccatt tgtagcgcca catcttctct ttcttcctcg ctgtccacga ttacctctgg 24540
tgatggcggg cgctcgggct tgggagaagg gcgcttcttt ttcttcttgg gcgcaatggc 24600
caaatccgcc gccgaggtcg atggccgcgg gctgggtgtg cgcggcacca gcgcgtcttg 24660
tgatgagtct tcctcgtcct cggactcgat acgccgcctc atccgctttt ttgggggcgc 24720
ccggggaggc ggcggcgacg gggacgggga cgacacgtcc tccatggttg ggggacgtcg 24780
cgccgcaccg cgtccgcgct cgggggtggt ttcgcgctgc tcctcttccc gactggccat 24840
ttccttctcc tataggcaga aaaagatcat ggagtcagtc gagaagaagg acagcctaac 24900
cgccccctct gagttcgcca ccaccgcctc caccgatgcc gccaacgcgc ctaccacctt 24960
ccccgtcgag gcacccccgc ttgaggagga ggaagtgatt atcgagcagg acccaggttt 25020
tgtaagcgaa gacgacgagg accgctcagt accaacagag gataaaaagc aagaccagga 25080
caacgcagag gcaaacgagg aacaagtcgg gcggggggac gaaaggcatg gcgactacct 25140
agatgtggga gacgacgtgc tgttgaagca tctgcagcgc cagtgcgcca ttatctgcga 25200
cgcgttgcaa gagcgcagcg atgtgcccct cgccatagcg gatgtcagcc ttgcctacga 25260
acgccaccta ttctcaccgc gcgtaccccc caaacgccaa gaaaacggca catgcgagcc 25320
caacccgcgc ctcaacttct accccgtatt tgccgtgcca gaggtgcttg ccacctatca 25380
catctttttc caaaactgca agatacccct atcctgccgt gccaaccgca gccgagcgga 25440
caagcagctg gccttgcggc agggcgctgt catacctgat atcgcctcgc tcaacgaagt 25500
gccaaaaatc tttgagggtc ttggacgcga cgagaagcgc gcggcaaacg ctctgcaaca 25560
ggaaaacagc gaaaatgaaa gtcactctgg agtgttggtg gaactcgagg gtgacaacgc 25620
gcgcctagcc gtactaaaac gcagcatcga ggtcacccac tttgcctacc cggcacttaa 25680
cctacccccc aaggtcatga gcacagtcat gagtgagctg atcgtgcgcc gtgcgcagcc 25740
cctggagagg gatgcaaatt tgcaagaaca aacagaggag ggcctacccg cagttggcga 25800
cgagcagcta gcgcgctggc ttcaaacgcg cgagcctgcc gacttggagg agcgacgcaa 25860
actaatgatg gccgcagtgc tcgttaccgt ggagcttgag tgcatgcagc ggttctttgc 25920
tgacccggag atgcagcgca agctagagga aacattgcac tacacctttc gacagggcta 25980
cgtacgccag gcctgcaaga tctccaacgt ggagctctgc aacctggtct cctaccttgg 26040
aattttgcac gaaaaccgcc ttgggcaaaa cgtgcttcat tccacgctca agggcgaggc 26100
gcgccgcgac tacgtccgcg actgcgttta cttatttcta tgctacacct ggcagacggc 26160
catgggcgtt tggcagcagt gcttggagga gtgcaacctc aaggagctgc agaaactgct 26220
aaagcaaaac ttgaaggacc tatggacggc cttcaacgag cgctccgtgg ccgcgcacct 26280
ggcggacatc attttccccg aacgcctgct taaaaccctg caacagggtc tgccagactt 26340
caccagtcaa agcatgttgc agaactttag gaactttatc ctagagcgct caggaatctt 26400
gcccgccacc tgctgtgcac ttcctagcga ctttgtgccc attaagtacc gcgaatgccc 26460
tccgccgctt tggggccact gctaccttct gcagctagcc aactaccttg cctaccactc 26520
tgacataatg gaagacgtga gcggtgacgg tctactggag tgtcactgtc gctgcaacct 26580
atgcaccccg caccgctccc tggtttgcaa ttcgcagctg cttaacgaaa gtcaaattat 26640
cggtaccttt gagctgcagg gtccctcgcc tgacgaaaag tccgcggctc cggggttgaa 26700
actcactccg gggctgtgga cgtcggctta ccttcgcaaa tttgtacctg aggactacca 26760
cgcccacgag attaggttct acgaagacca atcccgcccg cctaatgcgg agcttaccgc 26820
ctgcgtcatt acccagggcc acattcttgg ccaattgcaa gccatcaaca aagcccgcca 26880
agagtttctg ctacgaaagg gacggggggt ttacttggac ccccagtccg gcgaggagct 26940
caacccaatc cccccgccgc cgcagcccta tcagcagcag ccgcgggccc ttgcttccca 27000
ggatggcacc caaaaagaag ctgcagctgc cgccgccacc cacggacgag gaggaatact 27060
gggacagtca ggcagaggag gttttggacg aggaggagga ggacatgatg gaagactggg 27120
agagcctaga cgaggaagct tccgaggtcg aagaggtgtc agacgaaaca ccgtcaccct 27180
cggtcgcatt cccctcgccg gcgccccaga aatcggcaac cggttccagc atggctacaa 27240
cctccgctcc tcaggcgccg ccggcactgc ccgttcgccg acccaaccgt agatgggaca 27300
ccactggaac cagggccggt aagtccaagc agccgccgcc gttagcccaa gagcaacaac 27360
agcgccaagg ctaccgctca tggcgcgggc acaagaacgc catagttgct tgcttgcaag 27420
actgtggggg caacatctcc ttcgcccgcc gctttcttct ctaccatcac ggcgtggcct 27480
tcccccgtaa catcctgcat tactaccgtc atctctacag cccatactgc accggcggca 27540
gcggcagcaa cagcagcggc cacacagaag caaaggcgac cggatagcaa gactctgaca 27600
aagcccaaga aatccacagc ggcggcagca gcaggaggag gagcgctgcg tctggcgccc 27660
aacgaacccg tatcgacccg cgagcttaga aacaggattt ttcccactct gtatgctata 27720
tttcaacaga gcaggggcca agaacaagag ctgaaaataa aaaacaggtc tctgcgatcc 27780
ctcacccgca gctgcctgta tcacaaaagc gaagatcagc ttcggcgcac gctggaagac 27840
gcggaggctc tcttcagtaa atactgcgcg ctgactctta aggactagtt tcgcgccctt 27900
tctcaaattt aagcgcgaaa actacgtcat ctccagcggc cacacccggc gccagcacct 27960
gtcgtcagcg ccatttcaac tttgtataca aaagttgtga tgagcaagga aattcccacg 28020
ccctacatgt ggagttacca gccacaaatg ggacttgcgg ctggagctgc ccaagactac 28080
tcaacccgaa taaactacat gagcgcggga ccccacatga tatcccgggt caacggaata 28140
cgcgcccacc gaaaccgaat tctcctggaa caggcggcta ttaccaccac acctcgtaat 28200
aaccttaatc cccgtagttg gcccgctgcc ctggtgtacc aggaaagtcc cgctcccacc 28260
actgtggtac ttcccagaga cgcccaggcc gaagttcaga tgactaactc aggggcgcag 28320
cttgcgggcg gctttcgtca cagggtgcgg tcgcccgggc agggtataac tcacctgaca 28380
atcagagggc gaggtattca gctcaacgac gagtcggtga gctcctcgct tggtctccgt 28440
ccggacggga catttcagat cggcggcgcc ggccgctctt cattcacgcc tcgtcaggca 28500
atcctaactc tgcagacctc gtcctctgag ccgcgctctg gaggcattgg aactctgcaa 28560
tttattgagg agtttgtgcc atcggtctac tttaacccct tctcgggacc tcccggccac 28620
tatccggatc aatttattcc taactttgac gcggtaaagg actcggcgga cggctacgac 28680
tgaatgttaa gtggagaggc agagcaactg cgcctgaaac acctggtcca ctgtcgccgc 28740
cacaagtgct ttgcccgcga ctccggtgag ttttgctact ttgaattgcc cgaggatcat 28800
atcgagggcc cggcgcacgg cgtccggctt accgcccagg gagagcttgc ccgtagcctg 28860
attcgggagt ttacccagcg ccccctgcta gttgagcggg acaggggacc ctgtgttctc 28920
actgtgattt gcaactgtcc taaccctgga ttacatcaag atctttgttg ccatctctgt 28980
gctgagtata ataaatacag aaattaaaat atactggggc tcctatcgcc atcctgtaaa 29040
cgccaccgtc ttcacccgcc caagcaaacc aaggcgaacc ttacctggta cttttaacat 29100
ctctccctct gtgatttaca acagtttcaa cccagacgga gtgagtctac gagagaacct 29160
ctccgagctc agctactcca tcagaaaaaa caccaccctc cttacctgcc gggaacgtac 29220
gagtgcgtca ccggccgctg caccacacct accgcctgac cgtaaaccag actttttccg 29280
gacagacctc aataactctg tttaccagaa caggaggtga gcttagaaaa cccttagggt 29340
attaggccaa aggcgcagct actgtggggt ttatgaacaa ttcaagcaac tctacgggct 29400
attctaattc aggtttctct agaatcgggg ttggggttat tctctgtctt gtgattctct 29460
ttattcttat actaacgctt ctctgcctaa ggctcgccgc ctgctgtgtg cacatttgca 29520
tttattgtca gctttttaaa cgctggggtc gccacccaag atgattaggt acataatcct 29580
aggtttactc acccttgcgt cagcccacgg taccacccaa aaggtggatt ttaaggagcc 29640
agcctgtaat gttacattcg cagctgaagc taatgagtgc accactctta taaaatgcac 29700
cacagaacat gaaaagctgc ttattcgcca caaaaacaaa attggcaagt atgctgttta 29760
tgctatttgg cagccaggtg acactacaga gtataatgtt acagttttcc agggtaaaag 29820
tcataaaact tttatgtata cttttccatt ttatgaaatg tgcgacatta ccatgtacat 29880
gagcaaacag tataagttgt ggcccccaca aaattgtgtg gaaaacactg gcactttctg 29940
ctgcactgct atgctaatta cagtgctcgc tttggtctgt accctactct atattaaata 30000
caaaagcaga cgcagcttta ttgaggaaaa gaaaatgcct taatttacta agttacaaag 30060
ctaatgtcac cactaactgc tttactcgct gcttgcaaaa caaattcaaa aagttagcat 30120
tataattaga ataggattta aaccccccgg tcatttcctg ctcaatacca ttcccctgaa 30180
caattgactc tatgtgggat atgctccagc gctacaacct tgaagtcagg cttcctggat 30240
gtcagcatct gactttggcc agcacctgtc ccgcggattt gttccagtcc aactacagcg 30300
acccacccta acagagatga ccaacacaac caacgcggcc gccgctaccg gacttacatc 30360
taccacaaat acaccccaag tttctgcctt tgtcaataac tgggataact tgggcatgtg 30420
gtggttctcc atagcgctta tgtttgtatg ccttattatt atgtggctca tctgctgcct 30480
aaagcgcaaa cgcgcccgac cacccatcta tagtcccatc attgtgctac acccaaacaa 30540
tgatggaatc catagattgg acggactgaa acacatgttc ttttctctta cagtatgatt 30600
aaatgagaca tgattcctcg agtttttata ttactgaccc ttgttgcgct tttttgtgcg 30660
tgctccacat tggctgcggt ttctcacatc gaagtagact gcattccagc cttcacagtc 30720
tatttgcttt acggatttgt caccctcacg ctcatctgca gcctcatcac tgtggtcatc 30780
gcctttatcc agtgcattga ctgggtctgt gtgcgctttg catatctcag acaccatccc 30840
cagtacaggg acaggactat agctgagctt cttagaattc tttaattatg aaatttactg 30900
tgacttttct gctgattatt tgcaccctat ctgcgttttg ttccccgacc tccaagcctc 30960
aaagacatat atcatgcaga ttcactcgta tatggaatat tccaagttgc tacaatgaaa 31020
aaagcgatct ttccgaagcc tggttatatg caatcatctc tgttatggtg ttctgcagta 31080
ccatcttagc cctagctata tatccctacc ttgacattgg ctggaacgca atagatgcca 31140
tgaaccaccc aactttcccc gcgcccgcta tgcttccact gcaacaagtt gttgccggcg 31200
gctttgtccc agccaatcag cctcgcccac cttctcccac ccccactgaa atcagctact 31260
ttaatctaac aggaggagat gactgacacc ctagatctag aaatggacgg aattattaca 31320
gagcagcgcc tgctagaaag acgcagggca gcggccgagc aacagcgcat gaatcaagag 31380
ctccaagaca tggttaactt gcaccagtgc aaaaggggta tcttttgtct ggtaaagcag 31440
gccaaagtca cctacgacag taataccacc ggacaccgcc ttagctacaa gttgccaacc 31500
aagcgtcaga aattggtggt catggtggga gaaaagccca ttaccataac tcagcactcg 31560
gtagaaaccg aaggctgcat tcactcacct tgtcaaggac ctgaggatct ctgcaccctt 31620
attaagaccc tgtgcggtct caaagatctt attcccttta actaataaaa aaaaataata 31680
aagcatcact tacttaaaat cagttagcaa atttctgtcc agtttattca gcagcacctc 31740
cttgccctcc tcccagctct ggtattgcag cttcctcctg gctgcaaact ttctccacaa 31800
tctaaatgga atgtcagttt cctcctgttc ctgtccatcc gcacccacta tcttcatgtt 31860
gttgcagatg aagcgcgcaa gaccgtctga agataccttc aaccccgtgt atccatatga 31920
cacggaaacc ggtcctccaa ctgtgccttt tcttactcct ccctttgtat cccccaatgg 31980
gtttcaagag agtccccctg gggtactctc tttgcgccta tccgaacctc tagttacctc 32040
caatggcatg cttgcgctca aaatgggcaa cggcctctct ctggacgagg ccggcaacct 32100
tacctcccaa aatgtaacca ctgtgagccc acctctcaaa aaaaccaagt caaacataaa 32160
cctggaaata tctgcacccc tcacagttac ctcagaagcc ctaactgtgg ctgccgccgc 32220
acctctaatg gtcgcgggca acacactcac catgcaatca caggccccgc taaccgtgca 32280
cgactccaaa cttagcattg ccacccaagg acccctcaca gtgtcagaag gaaagctagc 32340
cctgcaaaca tcaggccccc tcaccaccac cgatagcagt acccttacta tcactgcctc 32400
accccctcta actactgcca ctggtagctt gggcattgac ttgaaagagc ccatttatac 32460
acaaaatgga aaactaggac taaagtacgg ggctcctttg catgtaacag acgacctaaa 32520
cactttgacc gtagcaactg gtccaggtgt gactattaat aatacttcct tgcaaactaa 32580
agttactgga gccttgggtt ttgattcaca aggcaatatg caacttaatg tagcaggagg 32640
actaaggatt gattctcaaa acagacgcct tatacttgat gttagttatc cgtttgatgc 32700
tcaaaaccaa ctaaatctaa gactaggaca gggccctctt tttataaact cagcccacaa 32760
cttggatatt aactacaaca aaggccttta cttgtttaca gcttcaaaca attccaaaaa 32820
gcttgaggtt aacctaagca ctgccaaggg gttgatgttt gacgctacag ccatagccat 32880
taatgcagga gatgggcttg aatttggttc acctaatgca ccaaacacaa atcccctcaa 32940
aacaaaaatt ggccatggcc tagaatttga ttcaaacaag gctatggttc ctaaactagg 33000
aactggcctt agttttgaca gcacaggtgc cattacagta ggaaacaaaa ataatgataa 33060
gctaactttg tggaccacac cagctccatc tcctaactgt agactaaatg cagagaaaga 33120
tgctaaactc actttggtct taacaaaatg tggcagtcaa atacttgcta cagtttcagt 33180
tttggctgtt aaaggcagtt tggctccaat atctggaaca gttcaaagtg ctcatcttat 33240
tataagattt gacgaaaatg gagtgctact aaacaattcc ttcctggacc cagaatattg 33300
gaactttaga aatggagatc ttactgaagg cacagcctat acaaacgctg ttggatttat 33360
gcctaaccta tcagcttatc caaaatctca cggtaaaact gccaaaagta acattgtcag 33420
tcaagtttac ttaaacggag acaaaactaa acctgtaaca ctaaccatta cactaaacgg 33480
tacacaggaa acaggagaca caactccaag tgcatactct atgtcatttt catgggactg 33540
gtctggccac aactacatta atgaaatatt tgccacatcc tcttacactt tttcatacat 33600
tgcccaagaa taaagaatcg tttgtgttat gtttcaacgt gtttattttt caattgcaga 33660
aaatttcgaa tcatttttca ttcagtagta tagccccacc accacatagc ttatacagat 33720
caccgtacct caactttgta taataaagtt gtaatcaaac tcacagaacc ctagtattca 33780
acctgccacc tccctcccaa cacacagagt acacagtcct ttctccccgg ctggccttaa 33840
aaagcatcat atcatgggta acagacatat tcttaggtgt tatattccac acggtttcct 33900
gtcgagccaa acgctcatca gtgatattaa taaactcccc gggcagctca cttaagttca 33960
tgtcgctgtc cagctgctga gccacaggct gctgtccaac ttgcggttgc ttaacgggcg 34020
gcgaaggaga agtccacgcc tacatggggg tagagtcata atcgtgcatc aggatagggc 34080
ggtggtgctg cagcagcgcg cgaataaact gctgccgccg ccgctccgtc ctgcaggaat 34140
acaacatggc agtggtctcc tcagcgatga ttcgcaccgc ccgcagcata aggcgccttg 34200
tcctccgggc acagcagcgc accctgatct cacttaaatc agcacagtaa ctgcagcaca 34260
gcaccacaat attgttcaaa atcccacagt gcaaggcgct gtatccaaag ctcatggcgg 34320
ggaccacaga acccacgtgg ccatcatacc acaagcgcag gtagattaag tggcgacccc 34380
tcataaacac gctggacata aacattacct cttttggcat gttgtaattc accacctccc 34440
ggtaccatat aaacctctga ttaaacatgg cgccatccac caccatccta aaccagctgg 34500
ccaaaacctg cccgccggct atacactgca gggaaccggg actggaacaa tgacagtgga 34560
gagcccagga ctcgtaacca tggatcatca tgctcgtcat gatatcaatg ttggcacaac 34620
acaggcacac gtgcatacac ttcctcagga ttacaagctc ctcccgcgtt agaaccatat 34680
cccagggaac aacccattcc tgaatcagcg taaatcccac actgcaggga agacctcgca 34740
cgtaactcac gttgtgcatt gtcaaagtgt tacattcggg cagcagcgga tgatcctcca 34800
gtatggtagc gcgggtttct gtctcaaaag gaggtagacg atccctactg tacggagtgc 34860
gccgagacaa ccgagatcgt gttggtcgta gtgtcatgcc aaatggaacg ccggacgtag 34920
tcatatttcc tgaagcaaaa ccaggtgcgg gcgtgacaaa cagatctgcg tctccggtct 34980
cgccgcttag atcgctctgt gtagtagttg tagtatatcc actctctcaa agcatccagg 35040
cgccccctgg cttcgggttc tatgtaaact ccttcatgcg ccgctgccct gataacatcc 35100
accaccgcag aataagccac acccagccaa cctacacatt cgttctgcga gtcacacacg 35160
ggaggagcgg gaagagctgg aagaaccatg tttttttttt tattccaaaa gattatccaa 35220
aacctcaaaa tgaagatcta ttaagtgaac gcgctcccct ccggtggcgt ggtcaaactc 35280
tacagccaaa gaacagataa tggcatttgt aagatgttgc acaatggctt ccaaaaggca 35340
aacggccctc acgtccaagt ggacgtaaag gctaaaccct tcagggtgaa tctcctctat 35400
aaacattcca gcaccttcaa ccatgcccaa ataattctca tctcgccacc ttctcaatat 35460
atctctaagc aaatcccgaa tattaagtcc ggccattgta aaaatctgct ccagagcgcc 35520
ctccaccttc agcctcaagc agcgaatcat gattgcaaaa attcaggttc ctcacagacc 35580
tgtataagat tcaaaagcgg aacattaaca aaaataccgc gatcccgtag gtcccttcgc 35640
agggccagct gaacataatc gtgcaggtct gcacggacca gcgcggccac ttccccgcca 35700
ggaaccatga caaaagaacc cacactgatt atgacacgca tactcggagc tatgctaacc 35760
agcgtagccc cgatgtaagc ttgttgcatg ggcggcgata taaaatgcaa ggtgctgctc 35820
aaaaaatcag gcaaagcctc gcgcaaaaaa gaaagcacat cgtagtcatg ctcatgcaga 35880
taaaggcagg taagctccgg aaccaccaca gaaaaagaca ccatttttct ctcaaacatg 35940
tctgcgggtt tctgcataaa cacaaaataa aataacaaaa aaacatttaa acattagaag 36000
cctgtcttac aacaggaaaa acaaccctta taagcataag acggactacg gccatgccgg 36060
cgtgaccgta aaaaaactgg tcaccgtgat taaaaagcac caccgacagc tcctcggtca 36120
tgtccggagt cataatgtaa gactcggtaa acacatcagg ttgattcaca tcggtcagtg 36180
ctaaaaagcg accgaaatag cccgggggaa tacatacccg caggcgtaga gacaacatta 36240
cagcccccat aggaggtata acaaaattaa taggagagaa aaacacataa acacctgaaa 36300
aaccctcctg cctaggcaaa atagcaccct cccgctccag aacaacatac agcgcttcca 36360
cagcggcagc cataacagtc agccttacca gtaaaaaaga aaacctatta aaaaaacacc 36420
actcgacacg gcaccagctc aatcagtcac agtgtaaaaa agggccaagt gcagagcgag 36480
tatatatagg actaaaaaat gacgtaacgg ttaaagtcca caaaaaacac ccagaaaacc 36540
gcacgcgaac ctacgcccag aaacgaaagc caaaaaaccc acaacttcct caaatcgtca 36600
cttccgtttt cccacgttac gtcacttccc attttaagaa aactacaatt cccaacacat 36660
acaagttact ccgccctaaa acctacgtca cccgccccgt tcccacgccc cgcgccacgt 36720
cacaaactcc accccctcat tatcatattg gcttcaatcc aaaataaggt atattattga 36780
tgatg 36785
<210> 7
<211> 36797
<212> DNA
<213> 人工序列
<220>
<223> 合成腺病毒基因组YPet-P2A-ADP
<400> 7
catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60
ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120
gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180
gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240
taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300
agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360
gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420
cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480
tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540
tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600
aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660
tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720
cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780
gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840
cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900
ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960
cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020
caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080
ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140
tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200
gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260
ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320
cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380
ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440
gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500
cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560
ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620
gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680
cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740
ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800
tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860
gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920
caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980
gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040
agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100
aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160
cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220
gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280
gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340
gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400
gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460
tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520
ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580
tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640
agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700
tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760
gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820
acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880
gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940
aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000
ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060
gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120
tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180
acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240
aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300
tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360
gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420
tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480
ttggctctag cgatgaagat acagattgag gtactgaaat gtgtgggcgt ggcttaaggg 3540
tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg 3600
ccgccgccca acttttctat acaaagttgt aatgagcacc aactcgtttg atggaagcat 3660
tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat 3720
gggctccagc attgatggtc gccccgtcct gcccgcaaac tctactacct tgacctacga 3780
gaccgtgtct ggaacgccgt tggagactgc agcctccgcc gccgcttcag ccgctgcagc 3840
caccgcccgc gggattgtga ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc 3900
ttcccgttca tccgcccgcg atgacaagtt gacggctctt ttggcacaat tggattcttt 3960
gacccgggaa cttaatgtcg tttctcagca gctgttggat ctgcgccagc aggtttctgc 4020
cctgaaggct tcctcccctc ccaatgcggt ttaaaacata aataaaaaac cagactctgt 4080
ttggatttgg atcaagcata agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg 4140
taggcccggg accagcggtc tcggtcgttg agggtcctgt gtattttttc caggacgtgg 4200
taaaggtgac tctggatgtt cagatacatg ggcataagcc cgtctctggg gtggaggtag 4260
caccactgca gagcttcatg ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag 4320
cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc tgattgccag gggcaggccc 4380
ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt gcatacgtgg ggatatgaga 4440
tgcatcttgg actgtatttt taggttggct atgttcccag ccatatccct ccggggattc 4500
atgttgtgca gaaccaccag cacagtgtat ccggtgcact tgggaaattt gtcatgtagc 4560
ttagaaggaa atgcgtggaa gaacttggag acgcccttgt gacctccaag attttccatg 4620
cattcgtcca taatgatggc aatgggccca cgggcggcgg cctgggcgaa gatatttctg 4680
ggatcactaa cgtcatagtt gtgttccagg atgagatcgt cataggccat ttttacaaag 4740
cgcgggcgga gggtgccaga ctgcggtata atggttccat ccggcccagg ggcgtagtta 4800
ccctcacaga tttgcatttc ccacgctttg agttcagatg gggggatcat gtctacctgc 4860
ggggcgatga agaaaacggt ttccggggta ggggagatca gctgggaaga aagcaggttc 4920
ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa tcacacctat taccgggtgc 4980
aactggtagt taagagagct gcagctgccg tcatccctga gcaggggggc cacttcgtta 5040
agcatgtccc tgactcgcat gttttccctg accaaatccg ccagaaggcg ctcgccgccc 5100
agcgatagca gttcttgcaa ggaagcaaag tttttcaacg gtttgagacc gtccgccgta 5160
ggcatgcttt tgagcgtttg accaagcagt tccaggcggt cccacagctc ggtcacctgc 5220
tctacggcat ctcgatccag catatctcct cgtttcgcgg gttggggcgg ctttcgctgt 5280
acggcagtag tcggtgctcg tccagacggg ccagggtcat gtctttccac gggcgcaggg 5340
tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc tccgggctgc gcgctggcca 5400
gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt 5460
cggccaggta gcatttgacc atggtgtcat agtccagccc ctccgcggcg tggcccttgg 5520
cgcgcagctt gcccttggag gaggcgccgc acgaggggca gtgcagactt ttgagggcgt 5580
agagcttggg cgcgagaaat accgattccg gggagtaggc atccgcgccg caggccccgc 5640
agacggtctc gcattccacg agccaggtga gctctggccg ttcggggtca aaaaccaggt 5700
ttcccccatg ctttttgatg cgtttcttac ctctggtttc catgagccgg tgtccacgct 5760
cggtgacgaa aaggctgtcc gtgtccccgt atacagactt gagaggcctg tcctcgagcg 5820
gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacaaag gctcgcgtcc 5880
aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact agggggtcca 5940
ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag gtgattggtt 6000
tgtaggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag ggggtggggg 6060
cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt tggggtgagt 6120
actccctctg aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg 6180
aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgca tccatctggt 6240
cagaaaagac aatctttttg ttgtcaagct tggtggcaaa cgacccgtag agggcgttgg 6300
acagcaactt ggcgatggag cgcagggttt ggtttttgtc gcgatcggcg cgctccttgg 6360
ccgcgatgtt tagctgcacg tattcgcgcg caacgcaccg ccattcggga aagacggtgg 6420
tgcgctcgtc gggcaccagg tgcacgcgcc aaccgcggtt gtgcagggtg acaaggtcaa 6480
cgctggtggc tacctctccg cgtaggcgct cgttggtcca gcagaggcgg ccgcccttgc 6540
gcgagcagaa tggcggtagg gggtctagct gcgtctcgtc cggggggtct gcgtccacgg 6600
taaagacccc gggcagcagg cgcgcgtcga agtagtctat cttgcatcct tgcaagtcta 6660
gcgcctgctg ccatgcgcgg gcggcaagcg cgcgctcgta tgggttgagt gggggacccc 6720
atggcatggg gtgggtgagc gcggaggcgt acatgccgca aatgtcgtaa acgtagaggg 6780
gctctctgag tattccaaga tatgtagggt agcatcttcc accgcggatg ctggcgcgca 6840
cgtaatcgta tagttcgtgc gagggagcga ggaggtcggg accgaggttg ctacgggcgg 6900
gctgctctgc tcggaagact atctgcctga agatggcatg tgagttggat gatatggttg 6960
gacgctggaa gacgttgaag ctggcgtctg tgagacctac cgcgtcacgc acgaaggagg 7020
cgtaggagtc gcgcagcttg ttgaccagct cggcggtgac ctgcacgtct agggcgcagt 7080
agtccagggt ttccttgatg atgtcatact tatcctgtcc cttttttttc cacagctcgc 7140
ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct 7200
ccgaacggta agagcctagc atgtagaact ggttgacggc ctggtaggcg cagcatccct 7260
tttctacggg tagcgcgtat gcctgcgcgg ccttccggag cgaggtgtgg gtgagcgcaa 7320
aggtgtccct gaccatgact ttgaggtact ggtatttgaa gtcagtgtcg tcgcatccgc 7380
cctgctccca gagcaaaaag tccgtgcgct ttttggaacg cggatttggc agggcgaagg 7440
tgacatcgtt gaagagtatc tttcccgcgc gaggcataaa gttgcgtgtg atgcggaagg 7500
gtcccggcac ctcggaacgg ttgttaatta cctgggcggc gagcacgatc tcgtcaaagc 7560
cgttgatgtt gtggcccaca atgtaaagtt ccaagaagcg cgggatgccc ttgatggaag 7620
gcaatttttt aagttcctcg taggtgagct cttcagggga gctgagcccg tgctctgaaa 7680
gggcccagtc tgcaagatga gggttggaag cgacgaatga gctccacagg tcacgggcca 7740
ttagcatttg caggtggtcg cgaaaggtcc taaactggcg acctatggcc attttttctg 7800
gggtgatgca gtagaaggta agcgggtctt gttcccagcg gtcccatcca aggttcgcgg 7860
ctaggtctcg cgcggcagtc actagaggct catctccgcc gaacttcatg accagcatga 7920
agggcacgag ctgcttccca aaggccccca tccaagtata ggtctctaca tcgtaggtga 7980
caaagagacg ctcggtgcga ggatgcgagc cgatcgggaa gaactggatc tcccgccacc 8040
aattggagga gtggctattg atgtggtgaa agtagaagtc cctgcgacgg gccgaacact 8100
cgtgctggct tttgtaaaaa cgtgcgcagt actggcagcg gtgcacgggc tgtacatcct 8160
gcacgaggtt gacctgacga ccgcgcacaa ggaagcagag tgggaatttg agcccctcgc 8220
ctggcgggtt tggctggtgg tcttctactt cggctgcttg tccttgaccg tctggctgct 8280
cgaggggagt tacggtggat cggaccacca cgccgcgcga gcccaaagtc cagatgtccg 8340
cgcgcggcgg tcggagcttg atgacaacat cgcgcagatg ggagctgtcc atggtctgga 8400
gctcccgcgg cgtcaggtca ggcgggagct cctgcaggtt tacctcgcat agacgggtca 8460
gggcgcgggc tagatccagg tgatacctaa tttccagggg ctggttggtg gcggcgtcga 8520
tggcttgcaa gaggccgcat ccccgcggcg cgactacggt accgcgcggc gggcggtggg 8580
ccgcgggggt gtccttggat gatgcatcta aaagcggtga cgcgggcgag cccccggagg 8640
tagggggggc tccggacccg ccgggagagg gggcaggggc acgtcggcgc cgcgcgcggg 8700
caggagctgg tgctgcgcgc gtaggttgct ggcgaacgcg acgacgcggc ggttgatctc 8760
ctgaatctgg cgcctctgcg tgaagacgac gggcccggtg agcttgagcc tgaaagagag 8820
ttcgacagaa tcaatttcgg tgtcgttgac ggcggcctgg cgcaaaatct cctgcacgtc 8880
tcctgagttg tcttgatagg cgatctcggc catgaactgc tcgatctctt cctcctggag 8940
atctccgcgt ccggctcgct ccacggtggc ggcgaggtcg ttggaaatgc gggccatgag 9000
ctgcgagaag gcgttgaggc ctccctcgtt ccagacgcgg ctgtagacca cgcccccttc 9060
ggcatcgcgg gcgcgcatga ccacctgcgc gagattgagc tccacgtgcc gggcgaagac 9120
ggcgtagttt cgcaggcgct gaaagaggta gttgagggtg gtggcggtgt gttctgccac 9180
gaagaagtac ataacccagc gtcgcaacgt ggattcgttg atatccccca aggcctcaag 9240
gcgctccatg gcctcgtaga agtccacggc gaagttgaaa aactgggagt tgcgcgccga 9300
cacggttaac tcctcctcca gaagacggat gagctcggcg acagtgtcgc gcacctcgcg 9360
ctcaaaggct acaggggcct cttcttcttc ttcaatctcc tcttccataa gggcctcccc 9420
ttcttcttct tctggcggcg gtgggggagg ggggacacgg cggcgacgac ggcgcaccgg 9480
gaggcggtcg acaaagcgct cgatcatctc cccgcggcga cggcgcatgg tctcggtgac 9540
ggcgcggccg ttctcgcggg ggcgcagttg gaagacgccg cccgtcatgt cccggttatg 9600
ggttggcggg gggctgccat gcggcaggga tacggcgcta acgatgcatc tcaacaattg 9660
ttgtgtaggt actccgccgc cgagggacct gagcgagtcc gcatcgaccg gatcggaaaa 9720
cctctcgaga aaggcgtcta accagtcaca gtcgcaaggt aggctgagca ccgtggcggg 9780
cggcagcggg cggcggtcgg ggttgtttct ggcggaggtg ctgctgatga tgtaattaaa 9840
gtaggcggtc ttgagacggc ggatggtcga cagaagcacc atgtccttgg gtccggcctg 9900
ctgaatgcgc aggcggtcgg ccatgcccca ggcttcgttt tgacatcggc gcaggtcttt 9960
gtagtagtct tgcatgagcc tttctaccgg cacttcttct tctccttcct cttgtcctgc 10020
atctcttgca tctatcgctg cggcggcggc ggagtttggc cgtaggtggc gccctcttcc 10080
tcccatgcgt gtgaccccga agcccctcat cggctgaagc agggctaggt cggcgacaac 10140
gcgctcggct aatatggcct gctgcacctg cgtgagggta gactggaagt catccatgtc 10200
cacaaagcgg tggtatgcgc ccgtgttgat ggtgtaagtg cagttggcca taacggacca 10260
gttaacggtc tggtgacccg gctgcgagag ctcggtgtac ctgagacgcg agtaagccct 10320
cgagtcaaat acgtagtcgt tgcaagtccg caccaggtac tggtatccca ccaaaaagtg 10380
cggcggcggc tggcggtaga ggggccagcg tagggtggcc ggggctccgg gggcgagatc 10440
ttccaacata aggcgatgat atccgtagat gtacctggac atccaggtga tgccggcggc 10500
ggtggtggag gcgcgcggaa agtcgcggac gcggttccag atgttgcgca gcggcaaaaa 10560
gtgctccatg gtcgggacgc tctggccggt caggcgcgcg caatcgttga cgctctagac 10620
cgtgcaaaag gagagcctgt aagcgggcac tcttccgtgg tctggtggat aaattcgcaa 10680
gggtatcatg gcggacgacc ggggttcgag ccccgtatcc ggccgtccgc cgtgatccat 10740
gcggttaccg cccgcgtgtc gaacccaggt gtgcgacgtc agacaacggg ggagtgctcc 10800
ttttggcttc cttccaggcg cggcggctgc tgcgctagct tttttggcca ctggccgcgc 10860
gcagcgtaag cggttaggct ggaaagcgaa agcattaagt ggctcgctcc ctgtagccgg 10920
agggttattt tccaagggtt gagtcgcggg acccccggtt cgagtctcgg accggccgga 10980
ctgcggcgaa cgggggtttg cctccccgtc atgcaagacc ccgcttgcaa attcctccgg 11040
aaacagggac gagccccttt tttgcttttc ccagatgcat ccggtgctgc ggcagatgcg 11100
cccccctcct cagcagcggc aagagcaaga gcagcggcag acatgcaggg caccctcccc 11160
tcctcctacc gcgtcaggag gggcgacatc cgcggttgac gcggcagcag atggtgatta 11220
cgaacccccg cggcgccggg cccggcacta cctggacttg gaggagggcg agggcctggc 11280
gcggctagga gcgccctctc ctgagcggta cccaagggtg cagctgaagc gtgatacgcg 11340
tgaggcgtac gtgccgcggc agaacctgtt tcgcgaccgc gagggagagg agcccgagga 11400
gatgcgggat cgaaagttcc acgcagggcg cgagctgcgg catggcctga atcgcgagcg 11460
gttgctgcgc gaggaggact ttgagcccga cgcgcgaacc gggattagtc ccgcgcgcgc 11520
acacgtggcg gccgccgacc tggtaaccgc atacgagcag acggtgaacc aggagattaa 11580
ctttcaaaaa agctttaaca accacgtgcg tacgcttgtg gcgcgcgagg aggtggctat 11640
aggactgatg catctgtggg actttgtaag cgcgctggag caaaacccaa atagcaagcc 11700
gctcatggcg cagctgttcc ttatagtgca gcacagcagg gacaacgagg cattcaggga 11760
tgcgctgcta aacatagtag agcccgaggg ccgctggctg ctcgatttga taaacatcct 11820
gcagagcata gtggtgcagg agcgcagctt gagcctggct gacaaggtgg ccgccatcaa 11880
ctattccatg cttagcctgg gcaagtttta cgcccgcaag atataccata ccccttacgt 11940
tcccatagac aaggaggtaa agatcgaggg gttctacatg cgcatggcgc tgaaggtgct 12000
taccttgagc gacgacctgg gcgtttatcg caacgagcgc atccacaagg ccgtgagcgt 12060
gagccggcgg cgcgagctca gcgaccgcga gctgatgcac agcctgcaaa gggccctggc 12120
tggcacgggc agcggcgata gagaggccga gtcctacttt gacgcgggcg ctgacctgcg 12180
ctgggcccca agccgacgcg ccctggaggc agctggggcc ggacctgggc tggcggtggc 12240
acccgcgcgc gctggcaacg tcggcggcgt ggaggaatat gacgaggacg atgagtacga 12300
gccagaggac ggcgagtact aagcggtgat gtttctgatc agatgatgca agacgcaacg 12360
gacccggcgg tgcgggcggc gctgcagagc cagccgtccg gccttaactc cacggacgac 12420
tggcgccagg tcatggaccg catcatgtcg ctgactgcgc gcaatcctga cgcgttccgg 12480
cagcagccgc aggccaaccg gctctccgca attctggaag cggtggtccc ggcgcgcgca 12540
aaccccacgc acgagaaggt gctggcgatc gtaaacgcgc tggccgaaaa cagggccatc 12600
cggcccgacg aggccggcct ggtctacgac gcgctgcttc agcgcgtggc tcgttacaac 12660
agcggcaacg tgcagaccaa cctggaccgg ctggtggggg atgtgcgcga ggccgtggcg 12720
cagcgtgagc gcgcgcagca gcagggcaac ctgggctcca tggttgcact aaacgccttc 12780
ctgagtacac agcccgccaa cgtgccgcgg ggacaggagg actacaccaa ctttgtgagc 12840
gcactgcggc taatggtgac tgagacaccg caaagtgagg tgtaccagtc tgggccagac 12900
tattttttcc agaccagtag acaaggcctg cagaccgtaa acctgagcca ggctttcaaa 12960
aacttgcagg ggctgtgggg ggtgcgggct cccacaggcg accgcgcgac cgtgtctagc 13020
ttgctgacgc ccaactcgcg cctgttgctg ctgctaatag cgcccttcac ggacagtggc 13080
agcgtgtccc gggacacata cctaggtcac ttgctgacac tgtaccgcga ggccataggt 13140
caggcgcatg tggacgagca tactttccag gagattacaa gtgtcagccg cgcgctgggg 13200
caggaggaca cgggcagcct ggaggcaacc ctaaactacc tgctgaccaa ccggcggcag 13260
aagatcccct cgttgcacag tttaaacagc gaggaggagc gcattttgcg ctacgtgcag 13320
cagagcgtga gccttaacct gatgcgcgac ggggtaacgc ccagcgtggc gctggacatg 13380
accgcgcgca acatggaacc gggcatgtat gcctcaaacc ggccgtttat caaccgccta 13440
atggactact tgcatcgcgc ggccgccgtg aaccccgagt atttcaccaa tgccatcttg 13500
aacccgcact ggctaccgcc ccctggtttc tacaccgggg gattcgaggt gcccgagggt 13560
aacgatggat tcctctggga cgacatagac gacagcgtgt tttccccgca accgcagacc 13620
ctgctagagt tgcaacagcg cgagcaggca gaggcggcgc tgcgaaagga aagcttccgc 13680
aggccaagca gcttgtccga tctaggcgct gcggccccgc ggtcagatgc tagtagccca 13740
tttccaagct tgatagggtc tcttaccagc actcgcacca cccgcccgcg cctgctgggc 13800
gaggaggagt acctaaacaa ctcgctgctg cagccgcagc gcgaaaaaaa cctgcctccg 13860
gcatttccca acaacgggat agagagccta gtggacaaga tgagtagatg gaagacgtac 13920
gcgcaggagc acagggacgt gccaggcccg cgcccgccca cccgtcgtca aaggcacgac 13980
cgtcagcggg gtctggtgtg ggaggacgat gactcggcag acgacagcag cgtcctggat 14040
ttgggaggga gtggcaaccc gtttgcgcac cttcgcccca ggctggggag aatgttttaa 14100
aaaaaaaaaa gcatgatgca aaataaaaaa ctcaccaagg ccatggcacc gagcgttggt 14160
tttcttgtat tccccttagt atgcggcgcg cggcgatgta tgaggaaggt cctcctccct 14220
cctacgagag tgtggtgagc gcggcgccag tggcggcggc gctgggttct cccttcgatg 14280
ctcccctgga cccgccgttt gtgcctccgc ggtacctgcg gcctaccggg gggagaaaca 14340
gcatccgtta ctctgagttg gcacccctat tcgacaccac ccgtgtgtac ctggtggaca 14400
acaagtcaac ggatgtggca tccctgaact accagaacga ccacagcaac tttctgacca 14460
cggtcattca aaacaatgac tacagcccgg gggaggcaag cacacagacc atcaatcttg 14520
acgaccggtc gcactggggc ggcgacctga aaaccatcct gcataccaac atgccaaatg 14580
tgaacgagtt catgtttacc aataagttta aggcgcgggt gatggtgtcg cgcttgccta 14640
ctaaggacaa tcaggtggag ctgaaatacg agtgggtgga gttcacgctg cccgagggca 14700
actactccga gaccatgacc atagacctta tgaacaacgc gatcgtggag cactacttga 14760
aagtgggcag acagaacggg gttctggaaa gcgacatcgg ggtaaagttt gacacccgca 14820
acttcagact ggggtttgac cccgtcactg gtcttgtcat gcctggggta tatacaaacg 14880
aagccttcca tccagacatc attttgctgc caggatgcgg ggtggacttc acccacagcc 14940
gcctgagcaa cttgttgggc atccgcaagc ggcaaccctt ccaggagggc tttaggatca 15000
cctacgatga tctggagggt ggtaacattc ccgcactgtt ggatgtggac gcctaccagg 15060
cgagcttgaa agatgacacc gaacagggcg ggggtggcgc aggcggcagc aacagcagtg 15120
gcagcggcgc ggaagagaac tccaacgcgg cagccgcggc aatgcagccg gtggaggaca 15180
tgaacgatca tgccattcgc ggcgacacct ttgccacacg ggctgaggag aagcgcgctg 15240
aggccgaagc agcggccgaa gctgccgccc ccgctgcgca acccgaggtc gagaagcctc 15300
agaagaaacc ggtgatcaaa cccctgacag aggacagcaa gaaacgcagt tacaacctaa 15360
taagcaatga cagcaccttc acccagtacc gcagctggta ccttgcatac aactacggcg 15420
accctcagac cggaatccgc tcatggaccc tgctttgcac tcctgacgta acctgcggct 15480
cggagcaggt ctactggtcg ttgccagaca tgatgcaaga ccccgtgacc ttccgctcca 15540
cgcgccagat cagcaacttt ccggtggtgg gcgccgagct gttgcccgtg cactccaaga 15600
gcttctacaa cgaccaggcc gtctactccc aactcatccg ccagtttacc tctctgaccc 15660
acgtgttcaa tcgctttccc gagaaccaga ttttggcgcg cccgccagcc cccaccatca 15720
ccaccgtcag tgaaaacgtt cctgctctca cagatcacgg gacgctaccg ctgcgcaaca 15780
gcatcggagg agtccagcga gtgaccatta ctgacgccag acgccgcacc tgcccctacg 15840
tttacaaggc cctgggcata gtctcgccgc gcgtcctatc gagccgcact ttttgagcaa 15900
gcatgtccat ccttatatcg cccagcaata acacaggctg gggcctgcgc ttcccaagca 15960
agatgtttgg cggggccaag aagcgctccg accaacaccc agtgcgcgtg cgcgggcact 16020
accgcgcgcc ctggggcgcg cacaaacgcg gccgcactgg gcgcaccacc gtcgatgacg 16080
ccatcgacgc ggtggtggag gaggcgcgca actacacgcc cacgccgcca ccagtgtcca 16140
cagtggacgc ggccattcag accgtggtgc gcggagcccg gcgctatgct aaaatgaaga 16200
gacggcggag gcgcgtagca cgtcgccacc gccgccgacc cggcactgcc gcccaacgcg 16260
cggcggcggc cctgcttaac cgcgcacgtc gcaccggccg acgggcggcc atgcgggccg 16320
ctcgaaggct ggccgcgggt attgtcactg tgccccccag gtccaggcga cgagcggccg 16380
ccgcagcagc cgcggccatt agtgctatga ctcagggtcg caggggcaac gtgtattggg 16440
tgcgcgactc ggttagcggc ctgcgcgtgc ccgtgcgcac ccgccccccg cgcaactaga 16500
ttgcaagaaa aaactactta gactcgtact gttgtatgta tccagcggcg gcggcgcgca 16560
acgaagctat gtccaagcgc aaaatcaaag aagagatgct ccaggtcatc gcgccggaga 16620
tctatggccc cccgaagaag gaagagcagg attacaagcc ccgaaagcta aagcgggtca 16680
aaaagaaaaa gaaagatgat gatgatgaac ttgacgacga ggtggaactg ctgcacgcta 16740
ccgcgcccag gcgacgggta cagtggaaag gtcgacgcgt aaaacgtgtt ttgcgacccg 16800
gcaccaccgt agtctttacg cccggtgagc gctccacccg cacctacaag cgcgtgtatg 16860
atgaggtgta cggcgacgag gacctgcttg agcaggccaa cgagcgcctc ggggagtttg 16920
cctacggaaa gcggcataag gacatgctgg cgttgccgct ggacgagggc aacccaacac 16980
ctagcctaaa gcccgtaaca ctgcagcagg tgctgcccgc gcttgcaccg tccgaagaaa 17040
agcgcggcct aaagcgcgag tctggtgact tggcacccac cgtgcagctg atggtaccca 17100
agcgccagcg actggaagat gtcttggaaa aaatgaccgt ggaacctggg ctggagcccg 17160
aggtccgcgt gcggccaatc aagcaggtgg cgccgggact gggcgtgcag accgtggacg 17220
ttcagatacc cactaccagt agcaccagta ttgccaccgc cacagagggc atggagacac 17280
aaacgtcccc ggttgcctca gcggtggcgg atgccgcggt gcaggcggtc gctgcggccg 17340
cgtccaagac ctctacggag gtgcaaacgg acccgtggat gtttcgcgtt tcagcccccc 17400
ggcgcccgcg cggttcgagg aagtacggcg ccgccagcgc gctactgccc gaatatgccc 17460
tacatccttc cattgcgcct acccccggct atcgtggcta cacctaccgc cccagaagac 17520
gagcaactac ccgacgccga accaccactg gaacccgccg ccgccgtcgc cgtcgccagc 17580
ccgtgctggc cccgatttcc gtgcgcaggg tggctcgcga aggaggcagg accctggtgc 17640
tgccaacagc gcgctaccac cccagcatcg tttaaaagcc ggtctttgtg gttcttgcag 17700
atatggccct cacctgccgc ctccgtttcc cggtgccggg attccgagga agaatgcacc 17760
gtaggagggg catggccggc cacggcctga cgggcggcat gcgtcgtgcg caccaccggc 17820
ggcggcgcgc gtcgcaccgt cgcatgcgcg gcggtatcct gcccctcctt attccactga 17880
tcgccgcggc gattggcgcc gtgcccggaa ttgcatccgt ggccttgcag gcgcagagac 17940
actgattaaa aacaagttgc atgtggaaaa atcaaaataa aaagtctgga ctctcacgct 18000
cgcttggtcc tgtaactatt ttgtagaatg gaagacatca actttgcgtc tctggccccg 18060
cgacacggct cgcgcccgtt catgggaaac tggcaagata tcggcaccag caatatgagc 18120
ggtggcgcct tcagctgggg ctcgctgtgg agcggcatta aaaatttcgg ttccaccgtt 18180
aagaactatg gcagcaaggc ctggaacagc agcacaggcc agatgctgag ggataagttg 18240
aaagagcaaa atttccaaca aaaggtggta gatggcctgg cctctggcat tagcggggtg 18300
gtggacctgg ccaaccaggc agtgcaaaat aagattaaca gtaagcttga tccccgccct 18360
cccgtagagg agcctccacc ggccgtggag acagtgtctc cagaggggcg tggcgaaaag 18420
cgtccgcgcc ccgacaggga agaaactctg gtgacgcaaa tagacgagcc tccctcgtac 18480
gaggaggcac taaagcaagg cctgcccacc acccgtccca tcgcgcccat ggctaccgga 18540
gtgctgggcc agcacacacc cgtaacgctg gacctgcctc cccccgccga cacccagcag 18600
aaacctgtgc tgccaggccc gaccgccgtt gttgtaaccc gtcctagccg cgcgtccctg 18660
cgccgcgccg ccagcggtcc gcgatcgttg cggcccgtag ccagtggcaa ctggcaaagc 18720
acactgaaca gcatcgtggg tctgggggtg caatccctga agcgccgacg atgcttctga 18780
atagctaacg tgtcgtatgt gtgtcatgta tgcgtccatg tcgccgccag aggagctgct 18840
gagccgccgc gcgcccgctt tccaagatgg ctaccccttc gatgatgccg cagtggtctt 18900
acatgcacat ctcgggccag gacgcctcgg agtacctgag ccccgggctg gtgcagtttg 18960
cccgcgccac cgagacgtac ttcagcctga ataacaagtt tagaaacccc acggtggcgc 19020
ctacgcacga cgtgaccaca gaccggtccc agcgtttgac gctgcggttc atccctgtgg 19080
accgtgagga tactgcgtac tcgtacaagg cgcggttcac cctagctgtg ggtgataacc 19140
gtgtgctgga catggcttcc acgtactttg acatccgcgg cgtgctggac aggggcccta 19200
cttttaagcc ctactctggc actgcctaca acgccctggc tcccaagggt gccccaaatc 19260
cttgcgaatg ggatgaagct gctactgctc ttgaaataaa cctagaagaa gaggacgatg 19320
acaacgaaga cgaagtagac gagcaagctg agcagcaaaa aactcacgta tttgggcagg 19380
cgccttattc tggtataaat attacaaagg agggtattca aataggtgtc gaaggtcaaa 19440
cacctaaata tgccgataaa acatttcaac ctgaacctca aataggagaa tctcagtggt 19500
acgaaactga aattaatcat gcagctggga gagtccttaa aaagactacc ccaatgaaac 19560
catgttacgg ttcatatgca aaacccacaa atgaaaatgg agggcaaggc attcttgtaa 19620
agcaacaaaa tggaaagcta gaaagtcaag tggaaatgca atttttctca actactgagg 19680
cgaccgcagg caatggtgat aacttgactc ctaaagtggt attgtacagt gaagatgtag 19740
atatagaaac cccagacact catatttctt acatgcccac tattaaggaa ggtaactcac 19800
gagaactaat gggccaacaa tctatgccca acaggcctaa ttacattgct tttagggaca 19860
attttattgg tctaatgtat tacaacagca cgggtaatat gggtgttctg gcgggccaag 19920
catcgcagtt gaatgctgtt gtagatttgc aagacagaaa cacagagctt tcataccagc 19980
ttttgcttga ttccattggt gatagaacca ggtacttttc tatgtggaat caggctgttg 20040
acagctatga tccagatgtt agaattattg aaaatcatgg aactgaagat gaacttccaa 20100
attactgctt tccactggga ggtgtgatta atacagagac tcttaccaag gtaaaaccta 20160
aaacaggtca ggaaaatgga tgggaaaaag atgctacaga attttcagat aaaaatgaaa 20220
taagagttgg aaataatttt gccatggaaa tcaatctaaa tgccaacctg tggagaaatt 20280
tcctgtactc caacatagcg ctgtatttgc ccgacaagct aaagtacagt ccttccaacg 20340
taaaaatttc tgataaccca aacacctacg actacatgaa caagcgagtg gtggctcccg 20400
ggttagtgga ctgctacatt aaccttggag cacgctggtc ccttgactat atggacaacg 20460
tcaacccatt taaccaccac cgcaatgctg gcctgcgcta ccgctcaatg ttgctgggca 20520
atggtcgcta tgtgcccttc cacatccagg tgcctcagaa gttctttgcc attaaaaacc 20580
tccttctcct gccgggctca tacacctacg agtggaactt caggaaggat gttaacatgg 20640
ttctgcagag ctccctagga aatgacctaa gggttgacgg agccagcatt aagtttgata 20700
gcatttgcct ttacgccacc ttcttcccca tggcccacaa caccgcctcc acgcttgagg 20760
ccatgcttag aaacgacacc aacgaccagt cctttaacga ctatctctcc gccgccaaca 20820
tgctctaccc tatacccgcc aacgctacca acgtgcccat atccatcccc tcccgcaact 20880
gggcggcttt ccgcggctgg gccttcacgc gccttaagac taaggaaacc ccatcactgg 20940
gctcgggcta cgacccttat tacacctact ctggctctat accctaccta gatggaacct 21000
tttacctcaa ccacaccttt aagaaggtgg ccattacctt tgactcttct gtcagctggc 21060
ctggcaatga ccgcctgctt acccccaacg agtttgaaat taagcgctca gttgacgggg 21120
agggttacaa cgttgcccag tgtaacatga ccaaagactg gttcctggta caaatgctag 21180
ctaactacaa cattggctac cagggcttct atatcccaga gagctacaag gaccgcatgt 21240
actccttctt tagaaacttc cagcccatga gccgtcaggt ggtggatgat actaaataca 21300
aggactacca acaggtgggc atcctacacc aacacaacaa ctctggattt gttggctacc 21360
ttgcccccac catgcgcgaa ggacaggcct accctgctaa cttcccctat ccgcttatag 21420
gcaagaccgc agttgacagc attacccaga aaaagtttct ttgcgatcgc accctttggc 21480
gcatcccatt ctccagtaac tttatgtcca tgggcgcact cacagacctg ggccaaaacc 21540
ttctctacgc caactccgcc cacgcgctag acatgacttt tgaggtggat cccatggacg 21600
agcccaccct tctttatgtt ttgtttgaag tctttgacgt ggtccgtgtg caccggccgc 21660
accgcggcgt catcgaaacc gtgtacctgc gcacgccctt ctcggccggc aacgccacaa 21720
cataaagaag caagcaacat caacaacagc tgccgccatg ggctccagtg agcaggaact 21780
gaaagccatt gtcaaagatc ttggttgtgg gccatatttt ttgggcacct atgacaagcg 21840
ctttccaggc tttgtttctc cacacaagct cgcctgcgcc atagtcaata cggccggtcg 21900
cgagactggg ggcgtacact ggatggcctt tgcctggaac ccgcactcaa aaacatgcta 21960
cctctttgag ccctttggct tttctgacca gcgactcaag caggtttacc agtttgagta 22020
cgagtcactc ctgcgccgta gcgccattgc ttcttccccc gaccgctgta taacgctgga 22080
aaagtccacc caaagcgtac aggggcccaa ctcggccgcc tgtggactat tctgctgcat 22140
gtttctccac gcctttgcca actggcccca aactcccatg gatcacaacc ccaccatgaa 22200
ccttattacc ggggtaccca actccatgct caacagtccc caggtacagc ccaccctgcg 22260
tcgcaaccag gaacagctct acagcttcct ggagcgccac tcgccctact tccgcagcca 22320
cagtgcgcag attaggagcg ccacttcttt ttgtcacttg aaaaacatgt aaaaataatg 22380
tactagagac actttcaata aaggcaaatg cttttatttg tacactctcg ggtgattatt 22440
tacccccacc cttgccgtct gcgccgttta aaaatcaaag gggttctgcc gcgcatcgct 22500
atgcgccact ggcagggaca cgttgcgata ctggtgttta gtgctccact taaactcagg 22560
cacaaccatc cgcggcagct cggtgaagtt ttcactccac aggctgcgca ccatcaccaa 22620
cgcgtttagc aggtcgggcg ccgatatctt gaagtcgcag ttggggcctc cgccctgcgc 22680
gcgcgagttg cgatacacag ggttgcagca ctggaacact atcagcgccg ggtggtgcac 22740
gctggccagc acgctcttgt cggagatcag atccgcgtcc aggtcctccg cgttgctcag 22800
ggcgaacgga gtcaactttg gtagctgcct tcccaaaaag ggcgcgtgcc caggctttga 22860
gttgcactcg caccgtagtg gcatcaaaag gtgaccgtgc ccggtctggg cgttaggata 22920
cagcgcctgc ataaaagcct tgatctgctt aaaagccacc tgagcctttg cgccttcaga 22980
gaagaacatg ccgcaagact tgccggaaaa ctgattggcc ggacaggccg cgtcgtgcac 23040
gcagcacctt gcgtcggtgt tggagatctg caccacattt cggccccacc ggttcttcac 23100
gatcttggcc ttgctagact gctccttcag cgcgcgctgc ccgttttcgc tcgtcacatc 23160
catttcaatc acgtgctcct tatttatcat aatgcttccg tgtagacact taagctcgcc 23220
ttcgatctca gcgcagcggt gcagccacaa cgcgcagccc gtgggctcgt gatgcttgta 23280
ggtcacctct gcaaacgact gcaggtacgc ctgcaggaat cgccccatca tcgtcacaaa 23340
ggtcttgttg ctggtgaagg tcagctgcaa cccgcggtgc tcctcgttca gccaggtctt 23400
gcatacggcc gccagagctt ccacttggtc aggcagtagt ttgaagttcg cctttagatc 23460
gttatccacg tggtacttgt ccatcagcgc gcgcgcagcc tccatgccct tctcccacgc 23520
agacacgatc ggcacactca gcgggttcat caccgtaatt tcactttccg cttcgctggg 23580
ctcttcctct tcctcttgcg tccgcatacc acgcgccact gggtcgtctt cattcagccg 23640
ccgcactgtg cgcttacctc ctttgccatg cttgattagc accggtgggt tgctgaaacc 23700
caccatttgt agcgccacat cttctctttc ttcctcgctg tccacgatta cctctggtga 23760
tggcgggcgc tcgggcttgg gagaagggcg cttctttttc ttcttgggcg caatggccaa 23820
atccgccgcc gaggtcgatg gccgcgggct gggtgtgcgc ggcaccagcg cgtcttgtga 23880
tgagtcttcc tcgtcctcgg actcgatacg ccgcctcatc cgcttttttg ggggcgcccg 23940
gggaggcggc ggcgacgggg acggggacga cacgtcctcc atggttgggg gacgtcgcgc 24000
cgcaccgcgt ccgcgctcgg gggtggtttc gcgctgctcc tcttcccgac tggccatttc 24060
cttctcctat aggcagaaaa agatcatgga gtcagtcgag aagaaggaca gcctaaccgc 24120
cccctctgag ttcgccacca ccgcctccac cgatgccgcc aacgcgccta ccaccttccc 24180
cgtcgaggca cccccgcttg aggaggagga agtgattatc gagcaggacc caggttttgt 24240
aagcgaagac gacgaggacc gctcagtacc aacagaggat aaaaagcaag accaggacaa 24300
cgcagaggca aacgaggaac aagtcgggcg gggggacgaa aggcatggcg actacctaga 24360
tgtgggagac gacgtgctgt tgaagcatct gcagcgccag tgcgccatta tctgcgacgc 24420
gttgcaagag cgcagcgatg tgcccctcgc catagcggat gtcagccttg cctacgaacg 24480
ccacctattc tcaccgcgcg taccccccaa acgccaagaa aacggcacat gcgagcccaa 24540
cccgcgcctc aacttctacc ccgtatttgc cgtgccagag gtgcttgcca cctatcacat 24600
ctttttccaa aactgcaaga tacccctatc ctgccgtgcc aaccgcagcc gagcggacaa 24660
gcagctggcc ttgcggcagg gcgctgtcat acctgatatc gcctcgctca acgaagtgcc 24720
aaaaatcttt gagggtcttg gacgcgacga gaagcgcgcg gcaaacgctc tgcaacagga 24780
aaacagcgaa aatgaaagtc actctggagt gttggtggaa ctcgagggtg acaacgcgcg 24840
cctagccgta ctaaaacgca gcatcgaggt cacccacttt gcctacccgg cacttaacct 24900
accccccaag gtcatgagca cagtcatgag tgagctgatc gtgcgccgtg cgcagcccct 24960
ggagagggat gcaaatttgc aagaacaaac agaggagggc ctacccgcag ttggcgacga 25020
gcagctagcg cgctggcttc aaacgcgcga gcctgccgac ttggaggagc gacgcaaact 25080
aatgatggcc gcagtgctcg ttaccgtgga gcttgagtgc atgcagcggt tctttgctga 25140
cccggagatg cagcgcaagc tagaggaaac attgcactac acctttcgac agggctacgt 25200
acgccaggcc tgcaagatct ccaacgtgga gctctgcaac ctggtctcct accttggaat 25260
tttgcacgaa aaccgccttg ggcaaaacgt gcttcattcc acgctcaagg gcgaggcgcg 25320
ccgcgactac gtccgcgact gcgtttactt atttctatgc tacacctggc agacggccat 25380
gggcgtttgg cagcagtgct tggaggagtg caacctcaag gagctgcaga aactgctaaa 25440
gcaaaacttg aaggacctat ggacggcctt caacgagcgc tccgtggccg cgcacctggc 25500
ggacatcatt ttccccgaac gcctgcttaa aaccctgcaa cagggtctgc cagacttcac 25560
cagtcaaagc atgttgcaga actttaggaa ctttatccta gagcgctcag gaatcttgcc 25620
cgccacctgc tgtgcacttc ctagcgactt tgtgcccatt aagtaccgcg aatgccctcc 25680
gccgctttgg ggccactgct accttctgca gctagccaac taccttgcct accactctga 25740
cataatggaa gacgtgagcg gtgacggtct actggagtgt cactgtcgct gcaacctatg 25800
caccccgcac cgctccctgg tttgcaattc gcagctgctt aacgaaagtc aaattatcgg 25860
tacctttgag ctgcagggtc cctcgcctga cgaaaagtcc gcggctccgg ggttgaaact 25920
cactccgggg ctgtggacgt cggcttacct tcgcaaattt gtacctgagg actaccacgc 25980
ccacgagatt aggttctacg aagaccaatc ccgcccgcca aatgcggagc ttaccgcctg 26040
cgtcattacc cagggccaca ttcttggcca attgcaagcc atcaacaaag cccgccaaga 26100
gtttctgcta cgaaagggac ggggggttta cttggacccc cagtccggcg aggagctcaa 26160
cccaatcccc ccgccgccgc agccctatca gcagcagccg cgggcccttg cttcccagga 26220
tggcacccaa aaagaagctg cagctgccgc cgccacccac ggacgaggag gaatactggg 26280
acagtcaggc agaggaggtt ttggacgagg aggaggagga catgatggaa gactgggaga 26340
gcctagacga ggaagcttcc gaggtcgaag aggtgtcaga cgaaacaccg tcaccctcgg 26400
tcgcattccc ctcgccggcg ccccagaaat cggcaaccgg ttccagcatg gctacaacct 26460
ccgctcctca ggcgccgccg gcactgcccg ttcgccgacc caaccgtaga tgggacacca 26520
ctggaaccag ggccggtaag tccaagcagc cgccgccgtt agcccaagag caacaacagc 26580
gccaaggcta ccgctcatgg cgcgggcaca agaacgccat agttgcttgc ttgcaagact 26640
gtgggggcaa catctccttc gcccgccgct ttcttctcta ccatcacggc gtggccttcc 26700
cccgtaacat cctgcattac taccgtcatc tctacagccc atactgcacc ggcggcagcg 26760
gcagcggcag caacagcagc ggccacacag aagcaaaggc gaccggatag caagactctg 26820
acaaagccca agaaatccac agcggcggca gcagcaggag gaggagcgct gcgtctggcg 26880
cccaacgaac ccgtatcgac ccgcgagctt agaaacagga tttttcccac tctgtatgct 26940
atatttcaac agagcagggg ccaagaacaa gagctgaaaa taaaaaacag gtctctgcga 27000
tccctcaccc gcagctgcct gtatcacaaa agcgaagatc agcttcggcg cacgctggaa 27060
gacgcggagg ctctcttcag taaatactgc gcgctgactc ttaaggacta gtttcgcgcc 27120
ctttctcaaa tttaagcgcg aaaactacgt catctccagc ggccacaccc ggcgccagca 27180
cctgtcgtca gcgccatttc aactttgtat acaaaagttg tgatgagcaa ggaaattccc 27240
acgccctaca tgtggagtta ccagccacaa atgggacttg cggctggagc tgcccaagac 27300
tactcaaccc gaataaacta catgagcgcg ggaccccaca tgatatcccg ggtcaacgga 27360
atacgcgccc accgaaaccg aattctcctg gaacaggcgg ctattaccac cacacctcgt 27420
aataacctta atccccgtag ttggcccgct gccctggtgt accaggaaag tcccgctccc 27480
accactgtgg tacttcccag agacgcccag gccgaagttc agatgactaa ctcaggggcg 27540
cagcttgcgg gcggctttcg tcacagggtg cggtcgcccg ggcagggtat aactcacctg 27600
acaatcagag ggcgaggtat tcagctcaac gacgagtcgg tgagctcctc gcttggtctc 27660
cgtccggacg ggacatttca gatcggcggc gccggccgct cttcattcac gcctcgtcag 27720
gcaatcctaa ctctgcagac ctcgtcctct gagccgcgct ctggaggcat tggaactctg 27780
caatttattg aggagtttgt gccatcggtc tactttaacc ccttctcggg acctcccggc 27840
cactatccgg atcaatttat tcctaacttt gacgcggtaa aggactcggc ggacggctac 27900
gactgaatgt taagtggaga ggcagagcaa ctgcgcctga aacacctggt ccactgtcgc 27960
cgccacaagt gctttgcccg cgactccggt gagttttgct actttgaatt gcccgaggat 28020
catatcgagg gcccggcgca cggcgtccgg cttaccgccc agggagagct tgcccgtagc 28080
ctgattcggg agtttaccca gcgccccctg ctagttgagc gggacagggg accctgtgtt 28140
ctcactgtga tttgcaactg tcctaaccct ggattacatc aagatctttg ttgccatctc 28200
tgtgctgagt ataataaata cagaaattaa aatatactgg ggctcctatc gccatcctgt 28260
aaacgccacc gtcttcaccc gcccaagcaa accaaggcga accttacctg gtacttttaa 28320
catctctccc tctgtgattt acaacagttt caacccagac ggagtgagtc tacgagagaa 28380
cctctccgag ctcagctact ccatcagaaa aaacaccacc ctccttacct gccgggaacg 28440
tacgagtgcg tcaccggccg ctgcaccaca cctaccgcct gaccgtaaac cagacttttt 28500
ccggacagac ctcaataact ctgtttacca gaacaggagg tgagcttaga aaacccttag 28560
ggtattaggc caaaggcgca gctactgtgg ggtttatgaa caattcaagc aactctacgg 28620
gctattctaa ttcaggtttc tctagaatcg gggttggggt tattctctgt cttgtgattc 28680
tctttattct tatactaacg cttctctgcc taaggctcgc cgcctgctgt gtgcacattt 28740
gcatttattg tcagcttttt aaacgctggg gtcgccaccc aagatgatta ggtacataat 28800
cctaggttta ctcacccttg cgtcagccca cggtaccacc caaaaggtgg attttaagga 28860
gccagcctgt aatgttacat tcgcagctga agctaatgag tgcaccactc ttataaaatg 28920
caccacagaa catgaaaagc tgcttattcg ccacaaaaac aaaattggca agtatgctgt 28980
ttatgctatt tggcagccag gtgacactac agagtataat gttacagttt tccagggtaa 29040
aagtcataaa acttttatgt atacttttcc attttatgaa atgtgcgaca ttaccatgta 29100
catgagcaaa cagtataagt tgtggccccc acaaaattgt gtggaaaaca ctggcacttt 29160
ctgctgcact gctatgctaa ttacagtgct cgctttggtc tgtaccctac tctatattaa 29220
atacaaaagc agacgcagct ttattgagga aaagaaaatg ccttaattta ctaagttaca 29280
aagctaatgt caccactaac tgctttactc gctgcttgca aaacaaattc aaaaagttag 29340
cattataatt agaataggat ttaaaccccc cggtcatttc ctgctcaata ccattcccct 29400
gaacaattga ctctatgtgg gatatgctcc agcgctacaa ccttgaagtc aggcttcctg 29460
gatgtcagca tctgactttg gccagcacct gtcccgcgga tttgttccag tccaactaca 29520
gcgacccacc ctaacagaga tggtgagcaa aggcgaagag ctgttcaccg gcgtggtgcc 29580
catcctggtg gagctggacg gcgacgtgaa cggccacaag ttcagcgtga gcggcgaggg 29640
cgagggcgac gccacctacg gcaagctgac cctgaagctg ctgtgcacca ccggcaagct 29700
gcccgtgccc tggcccaccc tggtgaccac cctgggctac ggcgtgcagt gcttcgcccg 29760
gtaccccgac cacatgaagc agcacgactt cttcaagagc gccatgcccg agggctacgt 29820
gcaggagcgg accatcttct tcaaggacga cggcaactac aagacccggg ccgaggtgaa 29880
gttcgagggc gacaccctgg tgaaccggat cgagctgaag ggcatcgact tcaaggagga 29940
cggcaacatc ctgggccaca agctggagta caactacaac agccacaacg tgtacatcac 30000
cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccggcaca acatcgagga 30060
cggcggcgtg cagctggccg accactacca gcagaacacc cccatcggcg acggccccgt 30120
gctgctgccc gacaaccact acctgagcta ccagagcgcc ctgttcaagg accccaacga 30180
gaagcgggac cacatggtgc tgctggagtt cctgaccgcc gccggcatca ccgagggcat 30240
gaacgagctc tataagggaa gcggagctac taacttcagc ctgctgaagc aggctggtga 30300
cgtcgaggag aatcctggcc caatggtgat gaccaacaca accaacgcgg ccgccgctac 30360
cggacttaca tctaccacaa atacacccca agtttctgcc tttgtcaata actgggataa 30420
cttgggcatg tggtggttct ccatagcgct tatgtttgta tgccttatta ttatgtggct 30480
catctgctgc ctaaagcgca aacgcgcccg accacccatc tatagtccca tcattgtgct 30540
acacccaaac aatgatggaa tccatagatt ggacggactg aaacacatgt tcttttctct 30600
tacagtatga ttaaatgaga catgattcct cgagttttta tattactgac ccttgttgcg 30660
cttttttgtg cgtgctccac attggctgcg gtttctcaca tcgaagtaga ctgcattcca 30720
gccttcacag tctatttgct ttacggattt gtcaccctca cgctcatctg cagcctcatc 30780
actgtggtca tcgcctttat ccagtgcatt gactgggtct gtgtgcgctt tgcatatctc 30840
agacaccatc cccagtacag ggacaggact atagctgagc ttcttagaat tctttaatta 30900
tgaaatttac tgtgactttt ctgctgatta tttgcaccct atctgcgttt tgttccccga 30960
cctccaagcc tcaaagacat atatcatgca gattcactcg tatatggaat attccaagtt 31020
gctacaatga aaaaagcgat ctttccgaag cctggttata tgcaatcatc tctgttatgg 31080
tgttctgcag taccatctta gccctagcta tatatcccta ccttgacatt ggctggaacg 31140
caatagatgc catgaaccac ccaactttcc ccgcgcccgc tatgcttcca ctgcaacaag 31200
ttgttgccgg cggctttgtc ccagccaatc agcctcgccc accttctccc acccccactg 31260
aaatcagcta ctttaatcta acaggaggag atgactgaca ccctagatct agaaatggac 31320
ggaattatta cagagcagcg cctgctagaa agacgcaggg cagcggccga gcaacagcgc 31380
atgaatcaag agctccaaga catggttaac ttgcaccagt gcaaaagggg tatcttttgt 31440
ctggtaaagc aggccaaagt cacctacgac agtaatacca ccggacaccg ccttagctac 31500
aagttgccaa ccaagcgtca gaaattggtg gtcatggtgg gagaaaagcc cattaccata 31560
actcagcact cggtagaaac cgaaggctgc attcactcac cttgtcaagg acctgaggat 31620
ctctgcaccc ttattaagac cctgtgcggt ctcaaagatc ttattccctt taactaataa 31680
aaaaaaataa taaagcatca cttacttaaa atcagttagc aaatttctgt ccagtttatt 31740
cagcagcacc tccttgccct cctcccagct ctggtattgc agcttcctcc tggctgcaaa 31800
ctttctccac aatctaaatg gaatgtcagt ttcctcctgt tcctgtccat ccgcacccac 31860
tatcttcatg ttgttgcaga tgaagcgcgc aagaccgtct gaagatacct tcaaccccgt 31920
gtatccatat gacacggaaa ccggtcctcc aactgtgcct tttcttactc ctccctttgt 31980
atcccccaat gggtttcaag agagtccccc tggggtactc tctttgcgcc tatccgaacc 32040
tctagttacc tccaatggca tgcttgcgct caaaatgggc aacggcctct ctctggacga 32100
ggccggcaac cttacctccc aaaatgtaac cactgtgagc ccacctctca aaaaaaccaa 32160
gtcaaacata aacctggaaa tatctgcacc cctcacagtt acctcagaag ccctaactgt 32220
ggctgccgcc gcacctctaa tggtcgcggg caacacactc accatgcaat cacaggcccc 32280
gctaaccgtg cacgactcca aacttagcat tgccacccaa ggacccctca cagtgtcaga 32340
aggaaagcta gccctgcaaa catcaggccc cctcaccacc accgatagca gtacccttac 32400
tatcactgcc tcaccccctc taactactgc cactggtagc ttgggcattg acttgaaaga 32460
gcccatttat acacaaaatg gaaaactagg actaaagtac ggggctcctt tgcatgtaac 32520
agacgaccta aacactttga ccgtagcaac tggtccaggt gtgactatta ataatacttc 32580
cttgcaaact aaagttactg gagccttggg ttttgattca caaggcaata tgcaacttaa 32640
tgtagcagga ggactaagga ttgattctca aaacagacgc cttatacttg atgttagtta 32700
tccgtttgat gctcaaaacc aactaaatct aagactagga cagggccctc tttttataaa 32760
ctcagcccac aacttggata ttaactacaa caaaggcctt tacttgttta cagcttcaaa 32820
caattccaaa aagcttgagg ttaacctaag cactgccaag gggttgatgt ttgacgctac 32880
agccatagcc attaatgcag gagatgggct tgaatttggt tcacctaatg caccaaacac 32940
aaatcccctc aaaacaaaaa ttggccatgg cctagaattt gattcaaaca aggctatggt 33000
tcctaaacta ggaactggcc ttagttttga cagcacaggt gccattacag taggaaacaa 33060
aaataatgat aagctaactt tgtggaccac accagctcca tctcctaact gtagactaaa 33120
tgcagagaaa gatgctaaac tcactttggt cttaacaaaa tgtggcagtc aaatacttgc 33180
tacagtttca gttttggctg ttaaaggcag tttggctcca atatctggaa cagttcaaag 33240
tgctcatctt attataagat ttgacgaaaa tggagtgcta ctaaacaatt ccttcctgga 33300
cccagaatat tggaacttta gaaatggaga tcttactgaa ggcacagcct atacaaacgc 33360
tgttggattt atgcctaacc tatcagctta tccaaaatct cacggtaaaa ctgccaaaag 33420
taacattgtc agtcaagttt acttaaacgg agacaaaact aaacctgtaa cactaaccat 33480
tacactaaac ggtacacagg aaacaggaga cacaactcca agtgcatact ctatgtcatt 33540
ttcatgggac tggtctggcc acaactacat taatgaaata tttgccacat cctcttacac 33600
tttttcatac attgcccaag aataaagaat cgtttgtgtt atgtttcaac gtgtttattt 33660
ttcaattgca gaaaatttca agtcattttt cattcagtag tatagcccca ccaccacata 33720
gcttatacag atcaccgtac ctcaactttg tataataaag ttgtaatcaa actcacagaa 33780
ccctagtatt caacctgcca cctccctccc aacacacaga gtacacagtc ctttctcccc 33840
ggctggcctt aaaaagcatc atatcatggg taacagacat attcttaggt gttatattcc 33900
acacggtttc ctgtcgagcc aaacgctcat cagtgatatt aataaactcc ccgggcagct 33960
cacttaagtt catgtcgctg tccagctgct gagccacagg ctgctgtcca acttgcggtt 34020
gcttaacggg cggcgaagga gaagtccacg cctacatggg ggtagagtca taatcgtgca 34080
tcaggatagg gcggtggtgc tgcagcagcg cgcgaataaa ctgctgccgc cgccgctccg 34140
tcctgcagga atacaacatg gcagtggtct cctcagcgat gattcgcacc gcccgcagca 34200
taaggcgcct tgtcctccgg gcacagcagc gcaccctgat ctcacttaaa tcagcacagt 34260
aactgcagca cagcaccaca atattgttca aaatcccaca gtgcaaggcg ctgtatccaa 34320
agctcatggc ggggaccaca gaacccacgt ggccatcata ccacaagcgc aggtagatta 34380
agtggcgacc cctcataaac acgctggaca taaacattac ctcttttggc atgttgtaat 34440
tcaccacctc ccggtaccat ataaacctct gattaaacat ggcgccatcc accaccatcc 34500
taaaccagct ggccaaaacc tgcccgccgg ctatacactg cagggaaccg ggactggaac 34560
aatgacagtg gagagcccag gactcgtaac catggatcat catgctcgtc atgatatcaa 34620
tgttggcaca acacaggcac acgtgcatac acttcctcag gattacaagc tcctcccgcg 34680
ttagaaccat atcccaggga acaacccatt cctgaatcag cgtaaatccc acactgcagg 34740
gaagacctcg cacgtaactc acgttgtgca ttgtcaaagt gttacattcg ggcagcagcg 34800
gatgatcctc cagtatggta gcgcgggttt ctgtctcaaa aggaggtaga cgatccctac 34860
tgtacggagt gcgccgagac aaccgagatc gtgttggtcg tagtgtcatg ccaaatggaa 34920
cgccggacgt agtcatattt cctgaagcaa aaccaggtgc gggcgtgaca aacagatctg 34980
cgtctccggt ctcgccgctt agatcgctct gtgtagtagt tgtagtatat ccactctctc 35040
aaagcatcca ggcgccccct ggcttcgggt tctatgtaaa ctccttcatg cgccgctgcc 35100
ctgataacat ccaccaccgc agaataagcc acacccagcc aacctacaca ttcgttctgc 35160
gagtcacaca cgggaggagc gggaagagct ggaagaacca tgtttttttt tttattccaa 35220
aagattatcc aaaacctcaa aatgaagatc tattaagtga acgcgctccc ctccggtggc 35280
gtggtcaaac tctacagcca aagaacagat aatggcattt gtaagatgtt gcacaatggc 35340
ttccaaaagg caaacggccc tcacgtccaa gtggacgtaa aggctaaacc cttcagggtg 35400
aatctcctct ataaacattc cagcaccttc aaccatgccc aaataattct catctcgcca 35460
ccttctcaat atatctctaa gcaaatcccg aatattaagt ccggccattg taaaaatctg 35520
ctccagagcg ccctccacct tcagcctcaa gcagcgaatc atgattgcaa aaattcaggt 35580
tcctcacaga cctgtataag attcaaaagc ggaacattaa caaaaatacc gcgatcccgt 35640
aggtcccttc gcagggccag ctgaacataa tcgtgcaggt ctgcacggac cagcgcggcc 35700
acttccccgc caggaaccat gacaaaagaa cccacactga ttatgacacg catactcgga 35760
gctatgctaa ccagcgtagc cccgatgtaa gcttgttgca tgggcggcga tataaaatgc 35820
aaggtgctgc tcaaaaaatc aggcaaagcc tcgcgcaaaa aagaaagcac atcgtagtca 35880
tgctcatgca gataaaggca ggtaagctcc ggaaccacca cagaaaaaga caccattttt 35940
ctctcaaaca tgtctgcggg tttctgcata aacacaaaat aaaataacaa aaaaacattt 36000
aaacattaga agcctgtctt acaacaggaa aaacaaccct tataagcata agacggacta 36060
cggccatgcc ggcgtgaccg taaaaaaact ggtcaccgtg attaaaaagc accaccgaca 36120
gctcctcggt catgtccgga gtcataatgt aagactcggt aaacacatca ggttgattca 36180
catcggtcag tgctaaaaag cgaccgaaat agcccggggg aatacatacc cgcaggcgta 36240
gagacaacat tacagccccc ataggaggta taacaaaatt aataggagag aaaaacacat 36300
aaacacctga aaaaccctcc tgcctaggca aaatagcacc ctcccgctcc agaacaacat 36360
acagcgcttc cacagcggca gccataacag tcagccttac cagtaaaaaa gaaaacctat 36420
taaaaaaaca ccactcgaca cggcaccagc tcaatcagtc acagtgtaaa aaagggccaa 36480
gtgcagagcg agtatatata ggactaaaaa atgacgtaac ggttaaagtc cacaaaaaac 36540
acccagaaaa ccgcacgcga acctacgccc agaaacgaaa gccaaaaaac ccacaacttc 36600
ctcaaatcgt cacttccgtt ttcccacgtt acgtcacttc ccattttaag aaaactacaa 36660
ttcccaacac atacaagtta ctccgcccta aaacctacgt cacccgcccc gttcccacgc 36720
cccgcgccac gtcacaaact ccaccccctc attatcatat tggcttcaat ccaaaataag 36780
gtatattatt gatgatg 36797
<210> 8
<211> 36791
<212> DNA
<213> 人工序列
<220>
<223> 合成腺病毒基因组ADP-P2A-YPet
<400> 8
catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60
ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120
gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180
gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240
taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300
agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360
gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420
cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480
tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540
tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600
aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660
tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720
cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780
gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840
cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900
ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960
cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020
caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080
ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140
tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200
gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260
ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320
cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380
ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440
gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500
cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560
ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620
gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680
cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740
ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800
tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860
gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920
caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980
gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040
agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100
aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160
cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220
gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280
gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340
gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400
gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460
tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520
ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580
tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640
agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700
tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760
gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820
acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880
gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940
aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000
ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060
gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120
tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180
acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240
aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300
tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360
gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420
tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480
ttggctctag cgatgaagat acagattgag gtactgaaat gtgtgggcgt ggcttaaggg 3540
tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg 3600
ccgccgccca acttttctat acaaagttgt aatgagcacc aactcgtttg atggaagcat 3660
tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat 3720
gggctccagc attgatggtc gccccgtcct gcccgcaaac tctactacct tgacctacga 3780
gaccgtgtct ggaacgccgt tggagactgc agcctccgcc gccgcttcag ccgctgcagc 3840
caccgcccgc gggattgtga ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc 3900
ttcccgttca tccgcccgcg atgacaagtt gacggctctt ttggcacaat tggattcttt 3960
gacccgggaa cttaatgtcg tttctcagca gctgttggat ctgcgccagc aggtttctgc 4020
cctgaaggct tcctcccctc ccaatgcggt ttaaaacata aataaaaaac cagactctgt 4080
ttggatttgg atcaagcata agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg 4140
taggcccggg accagcggtc tcggtcgttg agggtcctgt gtattttttc caggacgtgg 4200
taaaggtgac tctggatgtt cagatacatg ggcataagcc cgtctctggg gtggaggtag 4260
caccactgca gagcttcatg ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag 4320
cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc tgattgccag gggcaggccc 4380
ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt gcatacgtgg ggatatgaga 4440
tgcatcttgg actgtatttt taggttggct atgttcccag ccatatccct ccggggattc 4500
atgttgtgca gaaccaccag cacagtgtat ccggtgcact tgggaaattt gtcatgtagc 4560
ttagaaggaa atgcgtggaa gaacttggag acgcccttgt gacctccaag attttccatg 4620
cattcgtcca taatgatggc aatgggccca cgggcggcgg cctgggcgaa gatatttctg 4680
ggatcactaa cgtcatagtt gtgttccagg atgagatcgt cataggccat ttttacaaag 4740
cgcgggcgga gggtgccaga ctgcggtata atggttccat ccggcccagg ggcgtagtta 4800
ccctcacaga tttgcatttc ccacgctttg agttcagatg gggggatcat gtctacctgc 4860
ggggcgatga agaaaacggt ttccggggta ggggagatca gctgggaaga aagcaggttc 4920
ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa tcacacctat taccgggtgc 4980
aactggtagt taagagagct gcagctgccg tcatccctga gcaggggggc cacttcgtta 5040
agcatgtccc tgactcgcat gttttccctg accaaatccg ccagaaggcg ctcgccgccc 5100
agcgatagca gttcttgcaa ggaagcaaag tttttcaacg gtttgagacc gtccgccgta 5160
ggcatgcttt tgagcgtttg accaagcagt tccaggcggt cccacagctc ggtcacctgc 5220
tctacggcat ctcgatccag catatctcct cgtttcgcgg gttggggcgg ctttcgctgt 5280
acggcagtag tcggtgctcg tccagacggg ccagggtcat gtctttccac gggcgcaggg 5340
tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc tccgggctgc gcgctggcca 5400
gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt 5460
cggccaggta gcatttgacc atggtgtcat agtccagccc ctccgcggcg tggcccttgg 5520
cgcgcagctt gcccttggag gaggcgccgc acgaggggca gtgcagactt ttgagggcgt 5580
agagcttggg cgcgagaaat accgattccg gggagtaggc atccgcgccg caggccccgc 5640
agacggtctc gcattccacg agccaggtga gctctggccg ttcggggtca aaaaccaggt 5700
ttcccccatg ctttttgatg cgtttcttac ctctggtttc catgagccgg tgtccacgct 5760
cggtgacgaa aaggctgtcc gtgtccccgt atacagactt gagaggcctg tcctcgagcg 5820
gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacaaag gctcgcgtcc 5880
aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact agggggtcca 5940
ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag gtgattggtt 6000
tgtaggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag ggggtggggg 6060
cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt tggggtgagt 6120
actccctctg aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg 6180
aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgca tccatctggt 6240
cagaaaagac aatctttttg ttgtcaagct tggtggcaaa cgacccgtag agggcgttgg 6300
acagcaactt ggcgatggag cgcagggttt ggtttttgtc gcgatcggcg cgctccttgg 6360
ccgcgatgtt tagctgcacg tattcgcgcg caacgcaccg ccattcggga aagacggtgg 6420
tgcgctcgtc gggcaccagg tgcacgcgcc aaccgcggtt gtgcagggtg acaaggtcaa 6480
cgctggtggc tacctctccg cgtaggcgct cgttggtcca gcagaggcgg ccgcccttgc 6540
gcgagcagaa tggcggtagg gggtctagct gcgtctcgtc cggggggtct gcgtccacgg 6600
taaagacccc gggcagcagg cgcgcgtcga agtagtctat cttgcatcct tgcaagtcta 6660
gcgcctgctg ccatgcgcgg gcggcaagcg cgcgctcgta tgggttgagt gggggacccc 6720
atggcatggg gtgggtgagc gcggaggcgt acatgccgca aatgtcgtaa acgtagaggg 6780
gctctctgag tattccaaga tatgtagggt agcatcttcc accgcggatg ctggcgcgca 6840
cgtaatcgta tagttcgtgc gagggagcga ggaggtcggg accgaggttg ctacgggcgg 6900
gctgctctgc tcggaagact atctgcctga agatggcatg tgagttggat gatatggttg 6960
gacgctggaa gacgttgaag ctggcgtctg tgagacctac cgcgtcacgc acgaaggagg 7020
cgtaggagtc gcgcagcttg ttgaccagct cggcggtgac ctgcacgtct agggcgcagt 7080
agtccagggt ttccttgatg atgtcatact tatcctgtcc cttttttttc cacagctcgc 7140
ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct 7200
ccgaacggta agagcctagc atgtagaact ggttgacggc ctggtaggcg cagcatccct 7260
tttctacggg tagcgcgtat gcctgcgcgg ccttccggag cgaggtgtgg gtgagcgcaa 7320
aggtgtccct gaccatgact ttgaggtact ggtatttgaa gtcagtgtcg tcgcatccgc 7380
cctgctccca gagcaaaaag tccgtgcgct ttttggaacg cggatttggc agggcgaagg 7440
tgacatcgtt gaagagtatc tttcccgcgc gaggcataaa gttgcgtgtg atgcggaagg 7500
gtcccggcac ctcggaacgg ttgttaatta cctgggcggc gagcacgatc tcgtcaaagc 7560
cgttgatgtt gtggcccaca atgtaaagtt ccaagaagcg cgggatgccc ttgatggaag 7620
gcaatttttt aagttcctcg taggtgagct cttcagggga gctgagcccg tgctctgaaa 7680
gggcccagtc tgcaagatga gggttggaag cgacgaatga gctccacagg tcacgggcca 7740
ttagcatttg caggtggtcg cgaaaggtcc taaactggcg acctatggcc attttttctg 7800
gggtgatgca gtagaaggta agcgggtctt gttcccagcg gtcccatcca aggttcgcgg 7860
ctaggtctcg cgcggcagtc actagaggct catctccgcc gaacttcatg accagcatga 7920
agggcacgag ctgcttccca aaggccccca tccaagtata ggtctctaca tcgtaggtga 7980
caaagagacg ctcggtgcga ggatgcgagc cgatcgggaa gaactggatc tcccgccacc 8040
aattggagga gtggctattg atgtggtgaa agtagaagtc cctgcgacgg gccgaacact 8100
cgtgctggct tttgtaaaaa cgtgcgcagt actggcagcg gtgcacgggc tgtacatcct 8160
gcacgaggtt gacctgacga ccgcgcacaa ggaagcagag tgggaatttg agcccctcgc 8220
ctggcgggtt tggctggtgg tcttctactt cggctgcttg tccttgaccg tctggctgct 8280
cgaggggagt tacggtggat cggaccacca cgccgcgcga gcccaaagtc cagatgtccg 8340
cgcgcggcgg tcggagcttg atgacaacat cgcgcagatg ggagctgtcc atggtctgga 8400
gctcccgcgg cgtcaggtca ggcgggagct cctgcaggtt tacctcgcat agacgggtca 8460
gggcgcgggc tagatccagg tgatacctaa tttccagggg ctggttggtg gcggcgtcga 8520
tggcttgcaa gaggccgcat ccccgcggcg cgactacggt accgcgcggc gggcggtggg 8580
ccgcgggggt gtccttggat gatgcatcta aaagcggtga cgcgggcgag cccccggagg 8640
tagggggggc tccggacccg ccgggagagg gggcaggggc acgtcggcgc cgcgcgcggg 8700
caggagctgg tgctgcgcgc gtaggttgct ggcgaacgcg acgacgcggc ggttgatctc 8760
ctgaatctgg cgcctctgcg tgaagacgac gggcccggtg agcttgagcc tgaaagagag 8820
ttcgacagaa tcaatttcgg tgtcgttgac ggcggcctgg cgcaaaatct cctgcacgtc 8880
tcctgagttg tcttgatagg cgatctcggc catgaactgc tcgatctctt cctcctggag 8940
atctccgcgt ccggctcgct ccacggtggc ggcgaggtcg ttggaaatgc gggccatgag 9000
ctgcgagaag gcgttgaggc ctccctcgtt ccagacgcgg ctgtagacca cgcccccttc 9060
ggcatcgcgg gcgcgcatga ccacctgcgc gagattgagc tccacgtgcc gggcgaagac 9120
ggcgtagttt cgcaggcgct gaaagaggta gttgagggtg gtggcggtgt gttctgccac 9180
gaagaagtac ataacccagc gtcgcaacgt ggattcgttg atatccccca aggcctcaag 9240
gcgctccatg gcctcgtaga agtccacggc gaagttgaaa aactgggagt tgcgcgccga 9300
cacggttaac tcctcctcca gaagacggat gagctcggcg acagtgtcgc gcacctcgcg 9360
ctcaaaggct acaggggcct cttcttcttc ttcaatctcc tcttccataa gggcctcccc 9420
ttcttcttct tctggcggcg gtgggggagg ggggacacgg cggcgacgac ggcgcaccgg 9480
gaggcggtcg acaaagcgct cgatcatctc cccgcggcga cggcgcatgg tctcggtgac 9540
ggcgcggccg ttctcgcggg ggcgcagttg gaagacgccg cccgtcatgt cccggttatg 9600
ggttggcggg gggctgccat gcggcaggga tacggcgcta acgatgcatc tcaacaattg 9660
ttgtgtaggt actccgccgc cgagggacct gagcgagtcc gcatcgaccg gatcggaaaa 9720
cctctcgaga aaggcgtcta accagtcaca gtcgcaaggt aggctgagca ccgtggcggg 9780
cggcagcggg cggcggtcgg ggttgtttct ggcggaggtg ctgctgatga tgtaattaaa 9840
gtaggcggtc ttgagacggc ggatggtcga cagaagcacc atgtccttgg gtccggcctg 9900
ctgaatgcgc aggcggtcgg ccatgcccca ggcttcgttt tgacatcggc gcaggtcttt 9960
gtagtagtct tgcatgagcc tttctaccgg cacttcttct tctccttcct cttgtcctgc 10020
atctcttgca tctatcgctg cggcggcggc ggagtttggc cgtaggtggc gccctcttcc 10080
tcccatgcgt gtgaccccga agcccctcat cggctgaagc agggctaggt cggcgacaac 10140
gcgctcggct aatatggcct gctgcacctg cgtgagggta gactggaagt catccatgtc 10200
cacaaagcgg tggtatgcgc ccgtgttgat ggtgtaagtg cagttggcca taacggacca 10260
gttaacggtc tggtgacccg gctgcgagag ctcggtgtac ctgagacgcg agtaagccct 10320
cgagtcaaat acgtagtcgt tgcaagtccg caccaggtac tggtatccca ccaaaaagtg 10380
cggcggcggc tggcggtaga ggggccagcg tagggtggcc ggggctccgg gggcgagatc 10440
ttccaacata aggcgatgat atccgtagat gtacctggac atccaggtga tgccggcggc 10500
ggtggtggag gcgcgcggaa agtcgcggac gcggttccag atgttgcgca gcggcaaaaa 10560
gtgctccatg gtcgggacgc tctggccggt caggcgcgcg caatcgttga cgctctagac 10620
cgtgcaaaag gagagcctgt aagcgggcac tcttccgtgg tctggtggat aaattcgcaa 10680
gggtatcatg gcggacgacc ggggttcgag ccccgtatcc ggccgtccgc cgtgatccat 10740
gcggttaccg cccgcgtgtc gaacccaggt gtgcgacgtc agacaacggg ggagtgctcc 10800
ttttggcttc cttccaggcg cggcggctgc tgcgctagct tttttggcca ctggccgcgc 10860
gcagcgtaag cggttaggct ggaaagcgaa agcattaagt ggctcgctcc ctgtagccgg 10920
agggttattt tccaagggtt gagtcgcggg acccccggtt cgagtctcgg accggccgga 10980
ctgcggcgaa cgggggtttg cctccccgtc atgcaagacc ccgcttgcaa attcctccgg 11040
aaacagggac gagccccttt tttgcttttc ccagatgcat ccggtgctgc ggcagatgcg 11100
cccccctcct cagcagcggc aagagcaaga gcagcggcag acatgcaggg caccctcccc 11160
tcctcctacc gcgtcaggag gggcgacatc cgcggttgac gcggcagcag atggtgatta 11220
cgaacccccg cggcgccggg cccggcacta cctggacttg gaggagggcg agggcctggc 11280
gcggctagga gcgccctctc ctgagcggta cccaagggtg cagctgaagc gtgatacgcg 11340
tgaggcgtac gtgccgcggc agaacctgtt tcgcgaccgc gagggagagg agcccgagga 11400
gatgcgggat cgaaagttcc acgcagggcg cgagctgcgg catggcctga atcgcgagcg 11460
gttgctgcgc gaggaggact ttgagcccga cgcgcgaacc gggattagtc ccgcgcgcgc 11520
acacgtggcg gccgccgacc tggtaaccgc atacgagcag acggtgaacc aggagattaa 11580
ctttcaaaaa agctttaaca accacgtgcg tacgcttgtg gcgcgcgagg aggtggctat 11640
aggactgatg catctgtggg actttgtaag cgcgctggag caaaacccaa atagcaagcc 11700
gctcatggcg cagctgttcc ttatagtgca gcacagcagg gacaacgagg cattcaggga 11760
tgcgctgcta aacatagtag agcccgaggg ccgctggctg ctcgatttga taaacatcct 11820
gcagagcata gtggtgcagg agcgcagctt gagcctggct gacaaggtgg ccgccatcaa 11880
ctattccatg cttagcctgg gcaagtttta cgcccgcaag atataccata ccccttacgt 11940
tcccatagac aaggaggtaa agatcgaggg gttctacatg cgcatggcgc tgaaggtgct 12000
taccttgagc gacgacctgg gcgtttatcg caacgagcgc atccacaagg ccgtgagcgt 12060
gagccggcgg cgcgagctca gcgaccgcga gctgatgcac agcctgcaaa gggccctggc 12120
tggcacgggc agcggcgata gagaggccga gtcctacttt gacgcgggcg ctgacctgcg 12180
ctgggcccca agccgacgcg ccctggaggc agctggggcc ggacctgggc tggcggtggc 12240
acccgcgcgc gctggcaacg tcggcggcgt ggaggaatat gacgaggacg atgagtacga 12300
gccagaggac ggcgagtact aagcggtgat gtttctgatc agatgatgca agacgcaacg 12360
gacccggcgg tgcgggcggc gctgcagagc cagccgtccg gccttaactc cacggacgac 12420
tggcgccagg tcatggaccg catcatgtcg ctgactgcgc gcaatcctga cgcgttccgg 12480
cagcagccgc aggccaaccg gctctccgca attctggaag cggtggtccc ggcgcgcgca 12540
aaccccacgc acgagaaggt gctggcgatc gtaaacgcgc tggccgaaaa cagggccatc 12600
cggcccgacg aggccggcct ggtctacgac gcgctgcttc agcgcgtggc tcgttacaac 12660
agcggcaacg tgcagaccaa cctggaccgg ctggtggggg atgtgcgcga ggccgtggcg 12720
cagcgtgagc gcgcgcagca gcagggcaac ctgggctcca tggttgcact aaacgccttc 12780
ctgagtacac agcccgccaa cgtgccgcgg ggacaggagg actacaccaa ctttgtgagc 12840
gcactgcggc taatggtgac tgagacaccg caaagtgagg tgtaccagtc tgggccagac 12900
tattttttcc agaccagtag acaaggcctg cagaccgtaa acctgagcca ggctttcaaa 12960
aacttgcagg ggctgtgggg ggtgcgggct cccacaggcg accgcgcgac cgtgtctagc 13020
ttgctgacgc ccaactcgcg cctgttgctg ctgctaatag cgcccttcac ggacagtggc 13080
agcgtgtccc gggacacata cctaggtcac ttgctgacac tgtaccgcga ggccataggt 13140
caggcgcatg tggacgagca tactttccag gagattacaa gtgtcagccg cgcgctgggg 13200
caggaggaca cgggcagcct ggaggcaacc ctaaactacc tgctgaccaa ccggcggcag 13260
aagatcccct cgttgcacag tttaaacagc gaggaggagc gcattttgcg ctacgtgcag 13320
cagagcgtga gccttaacct gatgcgcgac ggggtaacgc ccagcgtggc gctggacatg 13380
accgcgcgca acatggaacc gggcatgtat gcctcaaacc ggccgtttat caaccgccta 13440
atggactact tgcatcgcgc ggccgccgtg aaccccgagt atttcaccaa tgccatcttg 13500
aacccgcact ggctaccgcc ccctggtttc tacaccgggg gattcgaggt gcccgagggt 13560
aacgatggat tcctctggga cgacatagac gacagcgtgt tttccccgca accgcagacc 13620
ctgctagagt tgcaacagcg cgagcaggca gaggcggcgc tgcgaaagga aagcttccgc 13680
aggccaagca gcttgtccga tctaggcgct gcggccccgc ggtcagatgc tagtagccca 13740
tttccaagct tgatagggtc tcttaccagc actcgcacca cccgcccgcg cctgctgggc 13800
gaggaggagt acctaaacaa ctcgctgctg cagccgcagc gcgaaaaaaa cctgcctccg 13860
gcatttccca acaacgggat agagagccta gtggacaaga tgagtagatg gaagacgtac 13920
gcgcaggagc acagggacgt gccaggcccg cgcccgccca cccgtcgtca aaggcacgac 13980
cgtcagcggg gtctggtgtg ggaggacgat gactcggcag acgacagcag cgtcctggat 14040
ttgggaggga gtggcaaccc gtttgcgcac cttcgcccca ggctggggag aatgttttaa 14100
aaaaaaaaaa gcatgatgca aaataaaaaa ctcaccaagg ccatggcacc gagcgttggt 14160
tttcttgtat tccccttagt atgcggcgcg cggcgatgta tgaggaaggt cctcctccct 14220
cctacgagag tgtggtgagc gcggcgccag tggcggcggc gctgggttct cccttcgatg 14280
ctcccctgga cccgccgttt gtgcctccgc ggtacctgcg gcctaccggg gggagaaaca 14340
gcatccgtta ctctgagttg gcacccctat tcgacaccac ccgtgtgtac ctggtggaca 14400
acaagtcaac ggatgtggca tccctgaact accagaacga ccacagcaac tttctgacca 14460
cggtcattca aaacaatgac tacagcccgg gggaggcaag cacacagacc atcaatcttg 14520
acgaccggtc gcactggggc ggcgacctga aaaccatcct gcataccaac atgccaaatg 14580
tgaacgagtt catgtttacc aataagttta aggcgcgggt gatggtgtcg cgcttgccta 14640
ctaaggacaa tcaggtggag ctgaaatacg agtgggtgga gttcacgctg cccgagggca 14700
actactccga gaccatgacc atagacctta tgaacaacgc gatcgtggag cactacttga 14760
aagtgggcag acagaacggg gttctggaaa gcgacatcgg ggtaaagttt gacacccgca 14820
acttcagact ggggtttgac cccgtcactg gtcttgtcat gcctggggta tatacaaacg 14880
aagccttcca tccagacatc attttgctgc caggatgcgg ggtggacttc acccacagcc 14940
gcctgagcaa cttgttgggc atccgcaagc ggcaaccctt ccaggagggc tttaggatca 15000
cctacgatga tctggagggt ggtaacattc ccgcactgtt ggatgtggac gcctaccagg 15060
cgagcttgaa agatgacacc gaacagggcg ggggtggcgc aggcggcagc aacagcagtg 15120
gcagcggcgc ggaagagaac tccaacgcgg cagccgcggc aatgcagccg gtggaggaca 15180
tgaacgatca tgccattcgc ggcgacacct ttgccacacg ggctgaggag aagcgcgctg 15240
aggccgaagc agcggccgaa gctgccgccc ccgctgcgca acccgaggtc gagaagcctc 15300
agaagaaacc ggtgatcaaa cccctgacag aggacagcaa gaaacgcagt tacaacctaa 15360
taagcaatga cagcaccttc acccagtacc gcagctggta ccttgcatac aactacggcg 15420
accctcagac cggaatccgc tcatggaccc tgctttgcac tcctgacgta acctgcggct 15480
cggagcaggt ctactggtcg ttgccagaca tgatgcaaga ccccgtgacc ttccgctcca 15540
cgcgccagat cagcaacttt ccggtggtgg gcgccgagct gttgcccgtg cactccaaga 15600
gcttctacaa cgaccaggcc gtctactccc aactcatccg ccagtttacc tctctgaccc 15660
acgtgttcaa tcgctttccc gagaaccaga ttttggcgcg cccgccagcc cccaccatca 15720
ccaccgtcag tgaaaacgtt cctgctctca cagatcacgg gacgctaccg ctgcgcaaca 15780
gcatcggagg agtccagcga gtgaccatta ctgacgccag acgccgcacc tgcccctacg 15840
tttacaaggc cctgggcata gtctcgccgc gcgtcctatc gagccgcact ttttgagcaa 15900
gcatgtccat ccttatatcg cccagcaata acacaggctg gggcctgcgc ttcccaagca 15960
agatgtttgg cggggccaag aagcgctccg accaacaccc agtgcgcgtg cgcgggcact 16020
accgcgcgcc ctggggcgcg cacaaacgcg gccgcactgg gcgcaccacc gtcgatgacg 16080
ccatcgacgc ggtggtggag gaggcgcgca actacacgcc cacgccgcca ccagtgtcca 16140
cagtggacgc ggccattcag accgtggtgc gcggagcccg gcgctatgct aaaatgaaga 16200
gacggcggag gcgcgtagca cgtcgccacc gccgccgacc cggcactgcc gcccaacgcg 16260
cggcggcggc cctgcttaac cgcgcacgtc gcaccggccg acgggcggcc atgcgggccg 16320
ctcgaaggct ggccgcgggt attgtcactg tgccccccag gtccaggcga cgagcggccg 16380
ccgcagcagc cgcggccatt agtgctatga ctcagggtcg caggggcaac gtgtattggg 16440
tgcgcgactc ggttagcggc ctgcgcgtgc ccgtgcgcac ccgccccccg cgcaactaga 16500
ttgcaagaaa aaactactta gactcgtact gttgtatgta tccagcggcg gcggcgcgca 16560
acgaagctat gtccaagcgc aaaatcaaag aagagatgct ccaggtcatc gcgccggaga 16620
tctatggccc cccgaagaag gaagagcagg attacaagcc ccgaaagcta aagcgggtca 16680
aaaagaaaaa gaaagatgat gatgatgaac ttgacgacga ggtggaactg ctgcacgcta 16740
ccgcgcccag gcgacgggta cagtggaaag gtcgacgcgt aaaacgtgtt ttgcgacccg 16800
gcaccaccgt agtctttacg cccggtgagc gctccacccg cacctacaag cgcgtgtatg 16860
atgaggtgta cggcgacgag gacctgcttg agcaggccaa cgagcgcctc ggggagtttg 16920
cctacggaaa gcggcataag gacatgctgg cgttgccgct ggacgagggc aacccaacac 16980
ctagcctaaa gcccgtaaca ctgcagcagg tgctgcccgc gcttgcaccg tccgaagaaa 17040
agcgcggcct aaagcgcgag tctggtgact tggcacccac cgtgcagctg atggtaccca 17100
agcgccagcg actggaagat gtcttggaaa aaatgaccgt ggaacctggg ctggagcccg 17160
aggtccgcgt gcggccaatc aagcaggtgg cgccgggact gggcgtgcag accgtggacg 17220
ttcagatacc cactaccagt agcaccagta ttgccaccgc cacagagggc atggagacac 17280
aaacgtcccc ggttgcctca gcggtggcgg atgccgcggt gcaggcggtc gctgcggccg 17340
cgtccaagac ctctacggag gtgcaaacgg acccgtggat gtttcgcgtt tcagcccccc 17400
ggcgcccgcg cggttcgagg aagtacggcg ccgccagcgc gctactgccc gaatatgccc 17460
tacatccttc cattgcgcct acccccggct atcgtggcta cacctaccgc cccagaagac 17520
gagcaactac ccgacgccga accaccactg gaacccgccg ccgccgtcgc cgtcgccagc 17580
ccgtgctggc cccgatttcc gtgcgcaggg tggctcgcga aggaggcagg accctggtgc 17640
tgccaacagc gcgctaccac cccagcatcg tttaaaagcc ggtctttgtg gttcttgcag 17700
atatggccct cacctgccgc ctccgtttcc cggtgccggg attccgagga agaatgcacc 17760
gtaggagggg catggccggc cacggcctga cgggcggcat gcgtcgtgcg caccaccggc 17820
ggcggcgcgc gtcgcaccgt cgcatgcgcg gcggtatcct gcccctcctt attccactga 17880
tcgccgcggc gattggcgcc gtgcccggaa ttgcatccgt ggccttgcag gcgcagagac 17940
actgattaaa aacaagttgc atgtggaaaa atcaaaataa aaagtctgga ctctcacgct 18000
cgcttggtcc tgtaactatt ttgtagaatg gaagacatca actttgcgtc tctggccccg 18060
cgacacggct cgcgcccgtt catgggaaac tggcaagata tcggcaccag caatatgagc 18120
ggtggcgcct tcagctgggg ctcgctgtgg agcggcatta aaaatttcgg ttccaccgtt 18180
aagaactatg gcagcaaggc ctggaacagc agcacaggcc agatgctgag ggataagttg 18240
aaagagcaaa atttccaaca aaaggtggta gatggcctgg cctctggcat tagcggggtg 18300
gtggacctgg ccaaccaggc agtgcaaaat aagattaaca gtaagcttga tccccgccct 18360
cccgtagagg agcctccacc ggccgtggag acagtgtctc cagaggggcg tggcgaaaag 18420
cgtccgcgcc ccgacaggga agaaactctg gtgacgcaaa tagacgagcc tccctcgtac 18480
gaggaggcac taaagcaagg cctgcccacc acccgtccca tcgcgcccat ggctaccgga 18540
gtgctgggcc agcacacacc cgtaacgctg gacctgcctc cccccgccga cacccagcag 18600
aaacctgtgc tgccaggccc gaccgccgtt gttgtaaccc gtcctagccg cgcgtccctg 18660
cgccgcgccg ccagcggtcc gcgatcgttg cggcccgtag ccagtggcaa ctggcaaagc 18720
acactgaaca gcatcgtggg tctgggggtg caatccctga agcgccgacg atgcttctga 18780
atagctaacg tgtcgtatgt gtgtcatgta tgcgtccatg tcgccgccag aggagctgct 18840
gagccgccgc gcgcccgctt tccaagatgg ctaccccttc gatgatgccg cagtggtctt 18900
acatgcacat ctcgggccag gacgcctcgg agtacctgag ccccgggctg gtgcagtttg 18960
cccgcgccac cgagacgtac ttcagcctga ataacaagtt tagaaacccc acggtggcgc 19020
ctacgcacga cgtgaccaca gaccggtccc agcgtttgac gctgcggttc atccctgtgg 19080
accgtgagga tactgcgtac tcgtacaagg cgcggttcac cctagctgtg ggtgataacc 19140
gtgtgctgga catggcttcc acgtactttg acatccgcgg cgtgctggac aggggcccta 19200
cttttaagcc ctactctggc actgcctaca acgccctggc tcccaagggt gccccaaatc 19260
cttgcgaatg ggatgaagct gctactgctc ttgaaataaa cctagaagaa gaggacgatg 19320
acaacgaaga cgaagtagac gagcaagctg agcagcaaaa aactcacgta tttgggcagg 19380
cgccttattc tggtataaat attacaaagg agggtattca aataggtgtc gaaggtcaaa 19440
cacctaaata tgccgataaa acatttcaac ctgaacctca aataggagaa tctcagtggt 19500
acgaaactga aattaatcat gcagctggga gagtccttaa aaagactacc ccaatgaaac 19560
catgttacgg ttcatatgca aaacccacaa atgaaaatgg agggcaaggc attcttgtaa 19620
agcaacaaaa tggaaagcta gaaagtcaag tggaaatgca atttttctca actactgagg 19680
cgaccgcagg caatggtgat aacttgactc ctaaagtggt attgtacagt gaagatgtag 19740
atatagaaac cccagacact catatttctt acatgcccac tattaaggaa ggtaactcac 19800
gagaactaat gggccaacaa tctatgccca acaggcctaa ttacattgct tttagggaca 19860
attttattgg tctaatgtat tacaacagca cgggtaatat gggtgttctg gcgggccaag 19920
catcgcagtt gaatgctgtt gtagatttgc aagacagaaa cacagagctt tcataccagc 19980
ttttgcttga ttccattggt gatagaacca ggtacttttc tatgtggaat caggctgttg 20040
acagctatga tccagatgtt agaattattg aaaatcatgg aactgaagat gaacttccaa 20100
attactgctt tccactggga ggtgtgatta atacagagac tcttaccaag gtaaaaccta 20160
aaacaggtca ggaaaatgga tgggaaaaag atgctacaga attttcagat aaaaatgaaa 20220
taagagttgg aaataatttt gccatggaaa tcaatctaaa tgccaacctg tggagaaatt 20280
tcctgtactc caacatagcg ctgtatttgc ccgacaagct aaagtacagt ccttccaacg 20340
taaaaatttc tgataaccca aacacctacg actacatgaa caagcgagtg gtggctcccg 20400
ggttagtgga ctgctacatt aaccttggag cacgctggtc ccttgactat atggacaacg 20460
tcaacccatt taaccaccac cgcaatgctg gcctgcgcta ccgctcaatg ttgctgggca 20520
atggtcgcta tgtgcccttc cacatccagg tgcctcagaa gttctttgcc attaaaaacc 20580
tccttctcct gccgggctca tacacctacg agtggaactt caggaaggat gttaacatgg 20640
ttctgcagag ctccctagga aatgacctaa gggttgacgg agccagcatt aagtttgata 20700
gcatttgcct ttacgccacc ttcttcccca tggcccacaa caccgcctcc acgcttgagg 20760
ccatgcttag aaacgacacc aacgaccagt cctttaacga ctatctctcc gccgccaaca 20820
tgctctaccc tatacccgcc aacgctacca acgtgcccat atccatcccc tcccgcaact 20880
gggcggcttt ccgcggctgg gccttcacgc gccttaagac taaggaaacc ccatcactgg 20940
gctcgggcta cgacccttat tacacctact ctggctctat accctaccta gatggaacct 21000
tttacctcaa ccacaccttt aagaaggtgg ccattacctt tgactcttct gtcagctggc 21060
ctggcaatga ccgcctgctt acccccaacg agtttgaaat taagcgctca gttgacgggg 21120
agggttacaa cgttgcccag tgtaacatga ccaaagactg gttcctggta caaatgctag 21180
ctaactacaa cattggctac cagggcttct atatcccaga gagctacaag gaccgcatgt 21240
actccttctt tagaaacttc cagcccatga gccgtcaggt ggtggatgat actaaataca 21300
aggactacca acaggtgggc atcctacacc aacacaacaa ctctggattt gttggctacc 21360
ttgcccccac catgcgcgaa ggacaggcct accctgctaa cttcccctat ccgcttatag 21420
gcaagaccgc agttgacagc attacccaga aaaagtttct ttgcgatcgc accctttggc 21480
gcatcccatt ctccagtaac tttatgtcca tgggcgcact cacagacctg ggccaaaacc 21540
ttctctacgc caactccgcc cacgcgctag acatgacttt tgaggtggat cccatggacg 21600
agcccaccct tctttatgtt ttgtttgaag tctttgacgt ggtccgtgtg caccggccgc 21660
accgcggcgt catcgaaacc gtgtacctgc gcacgccctt ctcggccggc aacgccacaa 21720
cataaagaag caagcaacat caacaacagc tgccgccatg ggctccagtg agcaggaact 21780
gaaagccatt gtcaaagatc ttggttgtgg gccatatttt ttgggcacct atgacaagcg 21840
ctttccaggc tttgtttctc cacacaagct cgcctgcgcc atagtcaata cggccggtcg 21900
cgagactggg ggcgtacact ggatggcctt tgcctggaac ccgcactcaa aaacatgcta 21960
cctctttgag ccctttggct tttctgacca gcgactcaag caggtttacc agtttgagta 22020
cgagtcactc ctgcgccgta gcgccattgc ttcttccccc gaccgctgta taacgctgga 22080
aaagtccacc caaagcgtac aggggcccaa ctcggccgcc tgtggactat tctgctgcat 22140
gtttctccac gcctttgcca actggcccca aactcccatg gatcacaacc ccaccatgaa 22200
ccttattacc ggggtaccca actccatgct caacagtccc caggtacagc ccaccctgcg 22260
tcgcaaccag gaacagctct acagcttcct ggagcgccac tcgccctact tccgcagcca 22320
cagtgcgcag attaggagcg ccacttcttt ttgtcacttg aaaaacatgt aaaaataatg 22380
tactagagac actttcaata aaggcaaatg cttttatttg tacactctcg ggtgattatt 22440
tacccccacc cttgccgtct gcgccgttta aaaatcaaag gggttctgcc gcgcatcgct 22500
atgcgccact ggcagggaca cgttgcgata ctggtgttta gtgctccact taaactcagg 22560
cacaaccatc cgcggcagct cggtgaagtt ttcactccac aggctgcgca ccatcaccaa 22620
cgcgtttagc aggtcgggcg ccgatatctt gaagtcgcag ttggggcctc cgccctgcgc 22680
gcgcgagttg cgatacacag ggttgcagca ctggaacact atcagcgccg ggtggtgcac 22740
gctggccagc acgctcttgt cggagatcag atccgcgtcc aggtcctccg cgttgctcag 22800
ggcgaacgga gtcaactttg gtagctgcct tcccaaaaag ggcgcgtgcc caggctttga 22860
gttgcactcg caccgtagtg gcatcaaaag gtgaccgtgc ccggtctggg cgttaggata 22920
cagcgcctgc ataaaagcct tgatctgctt aaaagccacc tgagcctttg cgccttcaga 22980
gaagaacatg ccgcaagact tgccggaaaa ctgattggcc ggacaggccg cgtcgtgcac 23040
gcagcacctt gcgtcggtgt tggagatctg caccacattt cggccccacc ggttcttcac 23100
gatcttggcc ttgctagact gctccttcag cgcgcgctgc ccgttttcgc tcgtcacatc 23160
catttcaatc acgtgctcct tatttatcat aatgcttccg tgtagacact taagctcgcc 23220
ttcgatctca gcgcagcggt gcagccacaa cgcgcagccc gtgggctcgt gatgcttgta 23280
ggtcacctct gcaaacgact gcaggtacgc ctgcaggaat cgccccatca tcgtcacaaa 23340
ggtcttgttg ctggtgaagg tcagctgcaa cccgcggtgc tcctcgttca gccaggtctt 23400
gcatacggcc gccagagctt ccacttggtc aggcagtagt ttgaagttcg cctttagatc 23460
gttatccacg tggtacttgt ccatcagcgc gcgcgcagcc tccatgccct tctcccacgc 23520
agacacgatc ggcacactca gcgggttcat caccgtaatt tcactttccg cttcgctggg 23580
ctcttcctct tcctcttgcg tccgcatacc acgcgccact gggtcgtctt cattcagccg 23640
ccgcactgtg cgcttacctc ctttgccatg cttgattagc accggtgggt tgctgaaacc 23700
caccatttgt agcgccacat cttctctttc ttcctcgctg tccacgatta cctctggtga 23760
tggcgggcgc tcgggcttgg gagaagggcg cttctttttc ttcttgggcg caatggccaa 23820
atccgccgcc gaggtcgatg gccgcgggct gggtgtgcgc ggcaccagcg cgtcttgtga 23880
tgagtcttcc tcgtcctcgg actcgatacg ccgcctcatc cgcttttttg ggggcgcccg 23940
gggaggcggc ggcgacgggg acggggacga cacgtcctcc atggttgggg gacgtcgcgc 24000
cgcaccgcgt ccgcgctcgg gggtggtttc gcgctgctcc tcttcccgac tggccatttc 24060
cttctcctat aggcagaaaa agatcatgga gtcagtcgag aagaaggaca gcctaaccgc 24120
cccctctgag ttcgccacca ccgcctccac cgatgccgcc aacgcgccta ccaccttccc 24180
cgtcgaggca cccccgcttg aggaggagga agtgattatc gagcaggacc caggttttgt 24240
aagcgaagac gacgaggacc gctcagtacc aacagaggat aaaaagcaag accaggacaa 24300
cgcagaggca aacgaggaac aagtcgggcg gggggacgaa aggcatggcg actacctaga 24360
tgtgggagac gacgtgctgt tgaagcatct gcagcgccag tgcgccatta tctgcgacgc 24420
gttgcaagag cgcagcgatg tgcccctcgc catagcggat gtcagccttg cctacgaacg 24480
ccacctattc tcaccgcgcg taccccccaa acgccaagaa aacggcacat gcgagcccaa 24540
cccgcgcctc aacttctacc ccgtatttgc cgtgccagag gtgcttgcca cctatcacat 24600
ctttttccaa aactgcaaga tacccctatc ctgccgtgcc aaccgcagcc gagcggacaa 24660
gcagctggcc ttgcggcagg gcgctgtcat acctgatatc gcctcgctca acgaagtgcc 24720
aaaaatcttt gagggtcttg gacgcgacga gaagcgcgcg gcaaacgctc tgcaacagga 24780
aaacagcgaa aatgaaagtc actctggagt gttggtggaa ctcgagggtg acaacgcgcg 24840
cctagccgta ctaaaacgca gcatcgaggt cacccacttt gcctacccgg cacttaacct 24900
accccccaag gtcatgagca cagtcatgag tgagctgatc gtgcgccgtg cgcagcccct 24960
ggagagggat gcaaatttgc aagaacaaac agaggagggc ctacccgcag ttggcgacga 25020
gcagctagcg cgctggcttc aaacgcgcga gcctgccgac ttggaggagc gacgcaaact 25080
aatgatggcc gcagtgctcg ttaccgtgga gcttgagtgc atgcagcggt tctttgctga 25140
cccggagatg cagcgcaagc tagaggaaac attgcactac acctttcgac agggctacgt 25200
acgccaggcc tgcaagatct ccaacgtgga gctctgcaac ctggtctcct accttggaat 25260
tttgcacgaa aaccgccttg ggcaaaacgt gcttcattcc acgctcaagg gcgaggcgcg 25320
ccgcgactac gtccgcgact gcgtttactt atttctatgc tacacctggc agacggccat 25380
gggcgtttgg cagcagtgct tggaggagtg caacctcaag gagctgcaga aactgctaaa 25440
gcaaaacttg aaggacctat ggacggcctt caacgagcgc tccgtggccg cgcacctggc 25500
ggacatcatt ttccccgaac gcctgcttaa aaccctgcaa cagggtctgc cagacttcac 25560
cagtcaaagc atgttgcaga actttaggaa ctttatccta gagcgctcag gaatcttgcc 25620
cgccacctgc tgtgcacttc ctagcgactt tgtgcccatt aagtaccgcg aatgccctcc 25680
gccgctttgg ggccactgct accttctgca gctagccaac taccttgcct accactctga 25740
cataatggaa gacgtgagcg gtgacggtct actggagtgt cactgtcgct gcaacctatg 25800
caccccgcac cgctccctgg tttgcaattc gcagctgctt aacgaaagtc aaattatcgg 25860
tacctttgag ctgcagggtc cctcgcctga cgaaaagtcc gcggctccgg ggttgaaact 25920
cactccgggg ctgtggacgt cggcttacct tcgcaaattt gtacctgagg actaccacgc 25980
ccacgagatt aggttctacg aagaccaatc ccgcccgcca aatgcggagc ttaccgcctg 26040
cgtcattacc cagggccaca ttcttggcca attgcaagcc atcaacaaag cccgccaaga 26100
gtttctgcta cgaaagggac ggggggttta cttggacccc cagtccggcg aggagctcaa 26160
cccaatcccc ccgccgccgc agccctatca gcagcagccg cgggcccttg cttcccagga 26220
tggcacccaa aaagaagctg cagctgccgc cgccacccac ggacgaggag gaatactggg 26280
acagtcaggc agaggaggtt ttggacgagg aggaggagga catgatggaa gactgggaga 26340
gcctagacga ggaagcttcc gaggtcgaag aggtgtcaga cgaaacaccg tcaccctcgg 26400
tcgcattccc ctcgccggcg ccccagaaat cggcaaccgg ttccagcatg gctacaacct 26460
ccgctcctca ggcgccgccg gcactgcccg ttcgccgacc caaccgtaga tgggacacca 26520
ctggaaccag ggccggtaag tccaagcagc cgccgccgtt agcccaagag caacaacagc 26580
gccaaggcta ccgctcatgg cgcgggcaca agaacgccat agttgcttgc ttgcaagact 26640
gtgggggcaa catctccttc gcccgccgct ttcttctcta ccatcacggc gtggccttcc 26700
cccgtaacat cctgcattac taccgtcatc tctacagccc atactgcacc ggcggcagcg 26760
gcagcggcag caacagcagc ggccacacag aagcaaaggc gaccggatag caagactctg 26820
acaaagccca agaaatccac agcggcggca gcagcaggag gaggagcgct gcgtctggcg 26880
cccaacgaac ccgtatcgac ccgcgagctt agaaacagga tttttcccac tctgtatgct 26940
atatttcaac agagcagggg ccaagaacaa gagctgaaaa taaaaaacag gtctctgcga 27000
tccctcaccc gcagctgcct gtatcacaaa agcgaagatc agcttcggcg cacgctggaa 27060
gacgcggagg ctctcttcag taaatactgc gcgctgactc ttaaggacta gtttcgcgcc 27120
ctttctcaaa tttaagcgcg aaaactacgt catctccagc ggccacaccc ggcgccagca 27180
cctgtcgtca gcgccatttc aactttgtat acaaaagttg tgatgagcaa ggaaattccc 27240
acgccctaca tgtggagtta ccagccacaa atgggacttg cggctggagc tgcccaagac 27300
tactcaaccc gaataaacta catgagcgcg ggaccccaca tgatatcccg ggtcaacgga 27360
atacgcgccc accgaaaccg aattctcctg gaacaggcgg ctattaccac cacacctcgt 27420
aataacctta atccccgtag ttggcccgct gccctggtgt accaggaaag tcccgctccc 27480
accactgtgg tacttcccag agacgcccag gccgaagttc agatgactaa ctcaggggcg 27540
cagcttgcgg gcggctttcg tcacagggtg cggtcgcccg ggcagggtat aactcacctg 27600
acaatcagag ggcgaggtat tcagctcaac gacgagtcgg tgagctcctc gcttggtctc 27660
cgtccggacg ggacatttca gatcggcggc gccggccgct cttcattcac gcctcgtcag 27720
gcaatcctaa ctctgcagac ctcgtcctct gagccgcgct ctggaggcat tggaactctg 27780
caatttattg aggagtttgt gccatcggtc tactttaacc ccttctcggg acctcccggc 27840
cactatccgg atcaatttat tcctaacttt gacgcggtaa aggactcggc ggacggctac 27900
gactgaatgt taagtggaga ggcagagcaa ctgcgcctga aacacctggt ccactgtcgc 27960
cgccacaagt gctttgcccg cgactccggt gagttttgct actttgaatt gcccgaggat 28020
catatcgagg gcccggcgca cggcgtccgg cttaccgccc agggagagct tgcccgtagc 28080
ctgattcggg agtttaccca gcgccccctg ctagttgagc gggacagggg accctgtgtt 28140
ctcactgtga tttgcaactg tcctaaccct ggattacatc aagatctttg ttgccatctc 28200
tgtgctgagt ataataaata cagaaattaa aatatactgg ggctcctatc gccatcctgt 28260
aaacgccacc gtcttcaccc gcccaagcaa accaaggcga accttacctg gtacttttaa 28320
catctctccc tctgtgattt acaacagttt caacccagac ggagtgagtc tacgagagaa 28380
cctctccgag ctcagctact ccatcagaaa aaacaccacc ctccttacct gccgggaacg 28440
tacgagtgcg tcaccggccg ctgcaccaca cctaccgcct gaccgtaaac cagacttttt 28500
ccggacagac ctcaataact ctgtttacca gaacaggagg tgagcttaga aaacccttag 28560
ggtattaggc caaaggcgca gctactgtgg ggtttatgaa caattcaagc aactctacgg 28620
gctattctaa ttcaggtttc tctagaatcg gggttggggt tattctctgt cttgtgattc 28680
tctttattct tatactaacg cttctctgcc taaggctcgc cgcctgctgt gtgcacattt 28740
gcatttattg tcagcttttt aaacgctggg gtcgccaccc aagatgatta ggtacataat 28800
cctaggttta ctcacccttg cgtcagccca cggtaccacc caaaaggtgg attttaagga 28860
gccagcctgt aatgttacat tcgcagctga agctaatgag tgcaccactc ttataaaatg 28920
caccacagaa catgaaaagc tgcttattcg ccacaaaaac aaaattggca agtatgctgt 28980
ttatgctatt tggcagccag gtgacactac agagtataat gttacagttt tccagggtaa 29040
aagtcataaa acttttatgt atacttttcc attttatgaa atgtgcgaca ttaccatgta 29100
catgagcaaa cagtataagt tgtggccccc acaaaattgt gtggaaaaca ctggcacttt 29160
ctgctgcact gctatgctaa ttacagtgct cgctttggtc tgtaccctac tctatattaa 29220
atacaaaagc agacgcagct ttattgagga aaagaaaatg ccttaattta ctaagttaca 29280
aagctaatgt caccactaac tgctttactc gctgcttgca aaacaaattc aaaaagttag 29340
cattataatt agaataggat ttaaaccccc cggtcatttc ctgctcaata ccattcccct 29400
gaacaattga ctctatgtgg gatatgctcc agcgctacaa ccttgaagtc aggcttcctg 29460
gatgtcagca tctgactttg gccagcacct gtcccgcgga tttgttccag tccaactaca 29520
gcgacccacc ctaacagaga tgaccaacac aaccaacgcg gccgccgcta ccggacttac 29580
atctaccaca aatacacccc aagtttctgc ctttgtcaat aactgggata acttgggcat 29640
gtggtggttc tccatagcgc ttatgtttgt atgccttatt attatgtggc tcatctgctg 29700
cctaaagcgc aaacgcgccc gaccacccat ctatagtccc atcattgtgc tacacccaaa 29760
caatgatgga atccatagat tggacggact gaaacacatg ttcttttctc ttacagtagg 29820
aagcggagct actaacttca gcctgctgaa gcaggctggt gacgtcgagg agaatcctgg 29880
cccaatggtg agcaaaggcg aagagctgtt caccggcgtg gtgcccatcc tggtggagct 29940
ggacggcgac gtgaacggcc acaagttcag cgtgagcggc gagggcgagg gcgacgccac 30000
ctacggcaag ctgaccctga agctgctgtg caccaccggc aagctgcccg tgccctggcc 30060
caccctggtg accaccctgg gctacggcgt gcagtgcttc gcccggtacc ccgaccacat 30120
gaagcagcac gacttcttca agagcgccat gcccgagggc tacgtgcagg agcggaccat 30180
cttcttcaag gacgacggca actacaagac ccgggccgag gtgaagttcg agggcgacac 30240
cctggtgaac cggatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg 30300
ccacaagctg gagtacaact acaacagcca caacgtgtac atcaccgccg acaagcagaa 30360
gaacggcatc aaggccaact tcaagatccg gcacaacatc gaggacggcg gcgtgcagct 30420
ggccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa 30480
ccactacctg agctaccaga gcgccctgtt caaggacccc aacgagaagc gggaccacat 30540
ggtgctgctg gagttcctga ccgccgccgg catcaccgag ggcatgaacg agctctataa 30600
gtgattaaat gagacatgat tcctcgagtt tttatattac tgacccttgt tgcgcttttt 30660
tgtgcgtgct ccacattggc tgcggtttct cacatcgaag tagactgcat tccagccttc 30720
acagtctatt tgctttacgg atttgtcacc ctcacgctca tctgcagcct catcactgtg 30780
gtcatcgcct ttatccagtg cattgactgg gtctgtgtgc gctttgcata tctcagacac 30840
catccccagt acagggacag gactatagct gagcttctta gaattcttta attatgaaat 30900
ttactgtgac ttttctgctg attatttgca ccctatctgc gttttgttcc ccgacctcca 30960
agcctcaaag acatatatca tgcagattca ctcgtatatg gaatattcca agttgctaca 31020
atgaaaaaag cgatctttcc gaagcctggt tatatgcaat catctctgtt atggtgttct 31080
gcagtaccat cttagcccta gctatatatc cctaccttga cattggctgg aacgcaatag 31140
atgccatgaa ccacccaact ttccccgcgc ccgctatgct tccactgcaa caagttgttg 31200
ccggcggctt tgtcccagcc aatcagcctc gcccaccttc tcccaccccc actgaaatca 31260
gctactttaa tctaacagga ggagatgact gacaccctag atctagaaat ggacggaatt 31320
attacagagc agcgcctgct agaaagacgc agggcagcgg ccgagcaaca gcgcatgaat 31380
caagagctcc aagacatggt taacttgcac cagtgcaaaa ggggtatctt ttgtctggta 31440
aagcaggcca aagtcaccta cgacagtaat accaccggac accgccttag ctacaagttg 31500
ccaaccaagc gtcagaaatt ggtggtcatg gtgggagaaa agcccattac cataactcag 31560
cactcggtag aaaccgaagg ctgcattcac tcaccttgtc aaggacctga ggatctctgc 31620
acccttatta agaccctgtg cggtctcaaa gatcttattc cctttaacta ataaaaaaaa 31680
ataataaagc atcacttact taaaatcagt tagcaaattt ctgtccagtt tattcagcag 31740
cacctccttg ccctcctccc agctctggta ttgcagcttc ctcctggctg caaactttct 31800
ccacaatcta aatggaatgt cagtttcctc ctgttcctgt ccatccgcac ccactatctt 31860
catgttgttg cagatgaagc gcgcaagacc gtctgaagat accttcaacc ccgtgtatcc 31920
atatgacacg gaaaccggtc ctccaactgt gccttttctt actcctccct ttgtatcccc 31980
caatgggttt caagagagtc cccctggggt actctctttg cgcctatccg aacctctagt 32040
tacctccaat ggcatgcttg cgctcaaaat gggcaacggc ctctctctgg acgaggccgg 32100
caaccttacc tcccaaaatg taaccactgt gagcccacct ctcaaaaaaa ccaagtcaaa 32160
cataaacctg gaaatatctg cacccctcac agttacctca gaagccctaa ctgtggctgc 32220
cgccgcacct ctaatggtcg cgggcaacac actcaccatg caatcacagg ccccgctaac 32280
cgtgcacgac tccaaactta gcattgccac ccaaggaccc ctcacagtgt cagaaggaaa 32340
gctagccctg caaacatcag gccccctcac caccaccgat agcagtaccc ttactatcac 32400
tgcctcaccc cctctaacta ctgccactgg tagcttgggc attgacttga aagagcccat 32460
ttatacacaa aatggaaaac taggactaaa gtacggggct cctttgcatg taacagacga 32520
cctaaacact ttgaccgtag caactggtcc aggtgtgact attaataata cttccttgca 32580
aactaaagtt actggagcct tgggttttga ttcacaaggc aatatgcaac ttaatgtagc 32640
aggaggacta aggattgatt ctcaaaacag acgccttata cttgatgtta gttatccgtt 32700
tgatgctcaa aaccaactaa atctaagact aggacagggc cctcttttta taaactcagc 32760
ccacaacttg gatattaact acaacaaagg cctttacttg tttacagctt caaacaattc 32820
caaaaagctt gaggttaacc taagcactgc caaggggttg atgtttgacg ctacagccat 32880
agccattaat gcaggagatg ggcttgaatt tggttcacct aatgcaccaa acacaaatcc 32940
cctcaaaaca aaaattggcc atggcctaga atttgattca aacaaggcta tggttcctaa 33000
actaggaact ggccttagtt ttgacagcac aggtgccatt acagtaggaa acaaaaataa 33060
tgataagcta actttgtgga ccacaccagc tccatctcct aactgtagac taaatgcaga 33120
gaaagatgct aaactcactt tggtcttaac aaaatgtggc agtcaaatac ttgctacagt 33180
ttcagttttg gctgttaaag gcagtttggc tccaatatct ggaacagttc aaagtgctca 33240
tcttattata agatttgacg aaaatggagt gctactaaac aattccttcc tggacccaga 33300
atattggaac tttagaaatg gagatcttac tgaaggcaca gcctatacaa acgctgttgg 33360
atttatgcct aacctatcag cttatccaaa atctcacggt aaaactgcca aaagtaacat 33420
tgtcagtcaa gtttacttaa acggagacaa aactaaacct gtaacactaa ccattacact 33480
aaacggtaca caggaaacag gagacacaac tccaagtgca tactctatgt cattttcatg 33540
ggactggtct ggccacaact acattaatga aatatttgcc acatcctctt acactttttc 33600
atacattgcc caagaataaa gaatcgtttg tgttatgttt caacgtgttt atttttcaat 33660
tgcagaaaat ttcgaatcat ttttcattca gtagtatagc cccaccacca catagcttat 33720
acagatcacc gtacctcaac tttgtataat aaagttgtaa tcaaactcac agaaccctag 33780
tattcaacct gccacctccc tcccaacaca cagagtacac agtcctttct ccccggctgg 33840
ccttaaaaag catcatatca tgggtaacag acatattctt aggtgttata ttccacacgg 33900
tttcctgtcg agccaaacgc tcatcagtga tattaataaa ctccccgggc agctcactta 33960
agttcatgtc gctgtccagc tgctgagcca caggctgctg tccaacttgc ggttgcttaa 34020
cgggcggcga aggagaagtc cacgcctaca tgggggtaga gtcataatcg tgcatcagga 34080
tagggcggtg gtgctgcagc agcgcgcgaa taaactgctg ccgccgccgc tccgtcctgc 34140
aggaatacaa catggcagtg gtctcctcag cgatgattcg caccgcccgc agcataaggc 34200
gccttgtcct ccgggcacag cagcgcaccc tgatctcact taaatcagca cagtaactgc 34260
agcacagcac cacaatattg ttcaaaatcc cacagtgcaa ggcgctgtat ccaaagctca 34320
tggcggggac cacagaaccc acgtggccat cataccacaa gcgcaggtag attaagtggc 34380
gacccctcat aaacacgctg gacataaaca ttacctcttt tggcatgttg taattcacca 34440
cctcccggta ccatataaac ctctgattaa acatggcgcc atccaccacc atcctaaacc 34500
agctggccaa aacctgcccg ccggctatac actgcaggga accgggactg gaacaatgac 34560
agtggagagc ccaggactcg taaccatgga tcatcatgct cgtcatgata tcaatgttgg 34620
cacaacacag gcacacgtgc atacacttcc tcaggattac aagctcctcc cgcgttagaa 34680
ccatatccca gggaacaacc cattcctgaa tcagcgtaaa tcccacactg cagggaagac 34740
ctcgcacgta actcacgttg tgcattgtca aagtgttaca ttcgggcagc agcggatgat 34800
cctccagtat ggtagcgcgg gtttctgtct caaaaggagg tagacgatcc ctactgtacg 34860
gagtgcgccg agacaaccga gatcgtgttg gtcgtagtgt catgccaaat ggaacgccgg 34920
acgtagtcat atttcctgaa gcaaaaccag gtgcgggcgt gacaaacaga tctgcgtctc 34980
cggtctcgcc gcttagatcg ctctgtgtag tagttgtagt atatccactc tctcaaagca 35040
tccaggcgcc ccctggcttc gggttctatg taaactcctt catgcgccgc tgccctgata 35100
acatccacca ccgcagaata agccacaccc agccaaccta cacattcgtt ctgcgagtca 35160
cacacgggag gagcgggaag agctggaaga accatgtttt tttttttatt ccaaaagatt 35220
atccaaaacc tcaaaatgaa gatctattaa gtgaacgcgc tcccctccgg tggcgtggtc 35280
aaactctaca gccaaagaac agataatggc atttgtaaga tgttgcacaa tggcttccaa 35340
aaggcaaacg gccctcacgt ccaagtggac gtaaaggcta aacccttcag ggtgaatctc 35400
ctctataaac attccagcac cttcaaccat gcccaaataa ttctcatctc gccaccttct 35460
caatatatct ctaagcaaat cccgaatatt aagtccggcc attgtaaaaa tctgctccag 35520
agcgccctcc accttcagcc tcaagcagcg aatcatgatt gcaaaaattc aggttcctca 35580
cagacctgta taagattcaa aagcggaaca ttaacaaaaa taccgcgatc ccgtaggtcc 35640
cttcgcaggg ccagctgaac ataatcgtgc aggtctgcac ggaccagcgc ggccacttcc 35700
ccgccaggaa ccatgacaaa agaacccaca ctgattatga cacgcatact cggagctatg 35760
ctaaccagcg tagccccgat gtaagcttgt tgcatgggcg gcgatataaa atgcaaggtg 35820
ctgctcaaaa aatcaggcaa agcctcgcgc aaaaaagaaa gcacatcgta gtcatgctca 35880
tgcagataaa ggcaggtaag ctccggaacc accacagaaa aagacaccat ttttctctca 35940
aacatgtctg cgggtttctg cataaacaca aaataaaata acaaaaaaac atttaaacat 36000
tagaagcctg tcttacaaca ggaaaaacaa cccttataag cataagacgg actacggcca 36060
tgccggcgtg accgtaaaaa aactggtcac cgtgattaaa aagcaccacc gacagctcct 36120
cggtcatgtc cggagtcata atgtaagact cggtaaacac atcaggttga ttcacatcgg 36180
tcagtgctaa aaagcgaccg aaatagcccg ggggaataca tacccgcagg cgtagagaca 36240
acattacagc ccccatagga ggtataacaa aattaatagg agagaaaaac acataaacac 36300
ctgaaaaacc ctcctgccta ggcaaaatag caccctcccg ctccagaaca acatacagcg 36360
cttccacagc ggcagccata acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa 36420
aacaccactc gacacggcac cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag 36480
agcgagtata tataggacta aaaaatgacg taacggttaa agtccacaaa aaacacccag 36540
aaaaccgcac gcgaacctac gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa 36600
tcgtcacttc cgttttccca cgttacgtca cttcccattt taagaaaact acaattccca 36660
acacatacaa gttactccgc cctaaaacct acgtcacccg ccccgttccc acgccccgcg 36720
ccacgtcaca aactccaccc cctcattatc atattggctt caatccaaaa taaggtatat 36780
tattgatgat g 36791
<210> 9
<211> 36720
<212> DNA
<213> 人工序列
<220>
<223> 合成腺病毒基因组E3-14.7k-P2A-YPet
<400> 9
catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60
ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120
gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180
gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240
taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300
agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360
gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420
cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480
tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540
tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600
aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660
tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720
cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780
gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840
cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900
ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960
cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020
caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080
ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140
tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200
gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260
ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320
cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380
ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440
gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500
cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560
ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620
gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680
cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740
ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800
tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860
gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920
caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980
gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040
agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100
aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160
cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220
gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280
gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340
gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400
gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460
tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520
ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580
tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640
agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700
tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760
gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820
acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880
gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940
aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000
ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060
gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120
tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180
acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240
aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300
tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360
gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420
tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480
ttggctctag cgatgaagat acagattgag gtactgaaat gtgtgggcgt ggcttaaggg 3540
tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg 3600
ccgccgccat gagcaccaac tcgtttgatg gaagcattgt gagctcatat ttgacaacgc 3660
gcatgccccc atgggccggg gtgcgtcaga atgtgatggg ctccagcatt gatggtcgcc 3720
ccgtcctgcc cgcaaactct actaccttga cctacgagac cgtgtctgga acgccgttgg 3780
agactgcagc ctccgccgcc gcttcagccg ctgcagccac cgcccgcggg attgtgactg 3840
actttgcttt cctgagcccg cttgcaagca gtgcagcttc ccgttcatcc gcccgcgatg 3900
acaagttgac ggctcttttg gcacaattgg attctttgac ccgggaactt aatgtcgttt 3960
ctcagcagct gttggatctg cgccagcagg tttctgccct gaaggcttcc tcccctccca 4020
atgcggttta aaacataaat aaaaaaccag actctgtttg gatttggatc aagcataagt 4080
gtcttgctgt ctttatttag gggttttgcg cgcgcggtag gcccgggacc agcggtctcg 4140
gtcgttgagg gtcctgtgta ttttttccag gacgtggtaa aggtgactct ggatgttcag 4200
atacatgggc ataagcccgt ctctggggtg gaggtagcac cactgcagag cttcatgctg 4260
cggggtggtg ttgtagatga tccagtcgta gcaggagcgc tgggcgtggt gcctaaaaat 4320
gtctttcagt agcaagctga ttgccagggg caggcccttg gtgtaagtgt ttacaaagcg 4380
gttaagctgg gatgggtgca tacgtgggga tatgagatgc atcttggact gtatttttag 4440
gttggctatg ttcccagcca tatccctccg gggattcatg ttgtgcagaa ccaccagcac 4500
agtgtatccg gtgcacttgg gaaatttgtc atgtagctta gaaggaaatg cgtggaagaa 4560
cttggagacg cccttgtgac ctccaagatt ttccatgcat tcgtccataa tgatggcaat 4620
gggcccacgg gcggcggcct gggcgaagat atttctggga tcactaacgt catagttgtg 4680
ttccaggatg agatcgtcat aggccatttt tacaaagcgc gggcggaggg tgccagactg 4740
cggtataatg gttccatccg gcccaggggc gtagttaccc tcacagattt gcatttccca 4800
cgctttgagt tcagatgggg ggatcatgtc tacctgcggg gcgatgaaga aaacggtttc 4860
cggggtaggg gagatcagct gggaagaaag caggttcctg agcagctgcg acttaccgca 4920
gccggtgggc ccgtaaatca cacctattac cggctgcaac tggtagttaa gagagctgca 4980
gctgccgtca tccctgagca ggggggccac ttcgttaagc atgtccctga ctcgcatgtt 5040
ttccctgacc aaatccgcca gaaggcgctc gccgcccagc gatagcagtt cttgcaagga 5100
agcaaagttt ttcaacggtt tgagaccgtc cgccgtaggc atgcttttga gcgtttgacc 5160
aagcagttcc aggcggtccc acagctcggt cacctgctct acggcatctc gatccagcat 5220
atctcctcgt ttcgcgggtt ggggcggctt tcgctgtacg gcagtagtcg gtgctcgtcc 5280
agacgggcca gggtcatgtc tttccacggg cgcagggtcc tcgtcagcgt agtctgggtc 5340
acggtgaagg ggtgcgctcc gggctgcgcg ctggccaggg tgcgcttgag gctggtcctg 5400
ctggtgctga agcgctgccg gtcttcgccc tgcgcgtcgg ccaggtagca tttgaccatg 5460
gtgtcatagt ccagcccctc cgcggcgtgg cccttggcgc gcagcttgcc cttggaggag 5520
gcgccgcacg aggggcagtg cagacttttg agggcgtaga gcttgggcgc gagaaatacc 5580
gattccgggg agtaggcatc cgcgccgcag gccccgcaga cggtctcgca ttccacgagc 5640
caggtgagct ctggccgttc ggggtcaaaa accaggtttc ccccatgctt tttgatgcgt 5700
ttcttacctc tggtttccat gagccggtgt ccacgctcgg tgacgaaaag gctgtccgtg 5760
tccccgtata cagacttgag aggcctgtcc tcgagcggtg ttccgcggtc ctcctcgtat 5820
agaaactcgg accactctga gacaaaggct cgcgtccagg ccagcacgaa ggaggctaag 5880
tgggaggggt agcggtcgtt gtccactagg gggtccactc gctccagggt gtgaagacac 5940
atgtcgccct cttcggcatc aaggaaggtg attggtttgt aggtgtaggc cacgtgaccg 6000
ggtgttcctg aaggggggct ataaaagggg gtgggggcgc gttcgtcctc actctcttcc 6060
gcatcgctgt ctgcgagggc cagctgttgg ggtgagtact ccctctgaaa agcgggcatg 6120
acttctgcgc taagattgtc agtttccaaa aacgaggagg atttgatatt cacctggccc 6180
gcggtgatgc ctttgagggt ggccgcatcc atctggtcag aaaagacaat ctttttgttg 6240
tcaagcttgg tggcaaacga cccgtagagg gcgttggaca gcaacttggc gatggagcgc 6300
agggtttggt ttttgtcgcg atcggcgcgc tccttggccg cgatgtttag ctgcacgtat 6360
tcgcgcgcaa cgcaccgcca ttcgggaaag acggtggtgc gctcgtcggg caccaggtgc 6420
acgcgccaac cgcggttgtg cagggtgaca aggtcaacgc tggtggctac ctctccgcgt 6480
aggcgctcgt tggtccagca gaggcggccg cccttgcgcg agcagaatgg cggtaggggg 6540
tctagctgcg tctcgtccgg ggggtctgcg tccacggtaa agaccccggg cagcaggcgc 6600
gcgtcgaagt agtctatctt gcatccttgc aagtctagcg cctgctgcca tgcgcgggcg 6660
gcaagcgcgc gctcgtatgg gttgagtggg ggaccccatg gcatggggtg ggtgagcgcg 6720
gaggcgtaca tgccgcaaat gtcgtaaacg tagaggggct ctctgagtat tccaagatat 6780
gtagggtagc atcttccacc gcggatgctg gcgcgcacgt aatcgtatag ttcgtgcgag 6840
ggagcgagga ggtcgggacc gaggttgcta cgggcgggct gctctgctcg gaagactatc 6900
tgcctgaaga tggcatgtga gttggatgat atggttggac gctggaagac gttgaagctg 6960
gcgtctgtga gacctaccgc gtcacgcacg aaggaggcgt aggagtcgcg cagcttgttg 7020
accagctcgg cggtgacctg cacgtctagg gcgcagtagt ccagggtttc cttgatgatg 7080
tcatacttat cctgtccctt ttttttccac agctcgcggt tgaggacaaa ctcttcgcgg 7140
tctttccagt actcttggat cggaaacccg tcggcctccg aacggtaaga gcctagcatg 7200
tagaactggt tgacggcctg gtaggcgcag catccctttt ctacgggtag cgcgtatgcc 7260
tgcgcggcct tccggagcga ggtgtgggtg agcgcaaagg tgtccctgac catgactttg 7320
aggtactggt atttgaagtc agtgtcgtcg catccgccct gctcccagag caaaaagtcc 7380
gtgcgctttt tggaacgcgg atttggcagg gcgaaggtga catcgttgaa gagtatcttt 7440
cccgcgcgag gcataaagtt gcgtgtgatg cggaagggtc ccggcacctc ggaacggttg 7500
ttaattacct gggcggcgag cacgatctcg tcaaagccgt tgatgttgtg gcccacaatg 7560
taaagttcca agaagcgcgg gatgcccttg atggaaggca attttttaag ttcctcgtag 7620
gtgagctctt caggggagct gagcccgtgc tctgaaaggg cccagtctgc aagatgaggg 7680
ttggaagcga cgaatgagct ccacaggtca cgggccatta gcatttgcag gtggtcgcga 7740
aaggtcctaa actggcgacc tatggccatt ttttctgggg tgatgcagta gaaggtaagc 7800
gggtcttgtt cccagcggtc ccatccaagg ttcgcggcta ggtctcgcgc ggcagtcact 7860
agaggctcat ctccgccgaa cttcatgacc agcatgaagg gcacgagctg cttcccaaag 7920
gcccccatcc aagtataggt ctctacatcg taggtgacaa agagacgctc ggtgcgagga 7980
tgcgagccga tcgggaagaa ctggatctcc cgccaccaat tggaggagtg gctattgatg 8040
tggtgaaagt agaagtccct gcgacgggcc gaacactcgt gctggctttt gtaaaaacgt 8100
gcgcagtact ggcagcggtg cacgggctgt acatcctgca cgaggttgac ctgacgaccg 8160
cgcacaagga agcagagtgg gaatttgagc ccctcgcctg gcgggtttgg ctggtggtct 8220
tctacttcgg ctgcttgtcc ttgaccgtct ggctgctcga ggggagttac ggtggatcgg 8280
accaccacgc cgcgcgagcc caaagtccag atgtccgcgc gcggcggtcg gagcttgatg 8340
acaacatcgc gcagatggga gctgtccatg gtctggagct cccgcggcgt caggtcaggc 8400
gggagctcct gcaggtttac ctcgcataga cgggtcaggg cgcgggctag atccaggtga 8460
tacctaattt ccaggggctg gttggtggcg gcgtcgatgg cttgcaagag gccgcatccc 8520
cgcggcgcga ctacggtacc gcgcggcggg cggtgggccg cgggggtgtc cttggatgat 8580
gcatctaaaa gcggtgacgc gggcgagccc ccggaggtag ggggggctcc ggacccgccg 8640
ggagaggggg caggggcacg tcggcgccgc gcgcgggcag gagctggtgc tgcgcgcgta 8700
ggttgctggc gaacgcgacg acgcggcggt tgatctcctg aatctggcgc ctctgcgtga 8760
agacgacggg cccggtgagc ttgaacctga aagagagttc gacagaatca atttcggtgt 8820
cgttgacggc ggcctggcgc aaaatctcct gcacgtctcc tgagttgtct tgataggcga 8880
tctcggccat gaactgctcg atctcttcct cctggagatc tccgcgtccg gctcgctcca 8940
cggtggcggc gaggtcgttg gaaatgcggg ccatgagctg cgagaaggcg ttgaggcctc 9000
cctcgttcca gacgcggctg tagaccacgc ccccttcggc atcgcgggcg cgcatgacca 9060
cctgcgcgag attgagctcc acgtgccggg cgaagacggc gtagtttcgc aggcgctgaa 9120
agaggtagtt gagggtggtg gcggtgtgtt ctgccacgaa gaagtacata acccagcgtc 9180
gcaacgtgga ttcgttgata tcccccaagg cctcaaggcg ctccatggcc tcgtagaagt 9240
ccacggcgaa gttgaaaaac tgggagttgc gcgccgacac ggttaactcc tcctccagaa 9300
gacggatgag ctcggcgaca gtgtcgcgca cctcgcgctc aaaggctaca ggggcctctt 9360
cttcttcttc aatctcctct tccataaggg cctccccttc ttcttcttct ggcggcggtg 9420
ggggaggggg gacacggcgg cgacgacggc gcaccgggag gcggtcgaca aagcgctcga 9480
tcatctcccc gcggcgacgg cgcatggtct cggtgacggc gcggccgttc tcgcgggggc 9540
gcagttggaa gacgccgccc gtcatgtccc ggttatgggt tggcgggggg ctgccatgcg 9600
gcagggatac ggcgctaacg atgcatctca acaattgttg tgtaggtact ccgccgccga 9660
gggacctgag cgagtccgca tcgaccggat cggaaaacct ctcgagaaag gcgtctaacc 9720
agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg cagcgggcgg cggtcggggt 9780
tgtttctggc ggaggtgctg ctgatgatgt aattaaagta ggcggtcttg agacggcgga 9840
tggtcgacag aagcaccatg tccttgggtc cggcctgctg aatgcgcagg cggtcggcca 9900
tgccccaggc ttcgttttga catcggcgca ggtctttgta gtagtcttgc atgagccttt 9960
ctaccggcac ttcttcttct ccttcctctt gtcctgcatc tcttgcatct atcgctgcgg 10020
cggcggcgga gtttggccgt aggtggcgcc ctcttcctcc catgcgtgtg accccgaagc 10080
ccctcatcgg ctgaagcagg gctaggtcgg cgacaacgcg ctcggctaat atggcctgct 10140
gcacctgcgt gagggtagac tggaagtcat ccatgtccac aaagcggtgg tatgcgcccg 10200
tgttgatggt gtaagtgcag ttggccataa cggaccagtt aacggtctgg tgacccggct 10260
gcgagagctc ggtgtacctg agacgcgagt aagccctcga gtcaaatacg tagtcgttgc 10320
aagtccgcac caggtactgg tatcccacca aaaagtgcgg cggcggctgg cggtagaggg 10380
gccagcgtag ggtggccggg gctccggggg cgagatcttc caacataagg cgatgatatc 10440
cgtagatgta cctggacatc caggtgatgc cggcggcggt ggtggaggcg cgcggaaagt 10500
cgcggacgcg gttccagatg ttgcgcagcg gcaaaaagtg ctccatggtc gggacgctct 10560
ggccggtcag gcgcgcgcaa tcgttgacgc tctagaccgt gcaaaaggag agcctgtaag 10620
cgggcactct tccgtggtct ggtggataaa ttcgcaaggg tatcatggcg gacgaccggg 10680
gttcgagccc cgtatccggc cgtccgccgt gatccatgcg gttaccgccc gcgtgtcgaa 10740
cccaggtgtg cgacgtcaga caacggggga gtgctccttt tggcttcctt ccaggcgcgg 10800
cggctgctgc gctagctttt ttggccactg gccgcgcgca gcgtaagcgg ttaggctgga 10860
aagcgaaagc attaagtggc tcgctccctg tagccggagg gttattttcc aagggttgag 10920
tcgcgggacc cccggttcga gtctcggacc ggccggactg cggcgaacgg gggtttgcct 10980
ccccgtcatg caagaccccg cttgcaaatt cctccggaaa cagggacgag cccctttttt 11040
gcttttccca gatgcatccg gtgctgcggc agatgcgccc ccctcctcag cagcggcaag 11100
agcaagagca gcggcagaca tgcagggcac cctcccctcc tcctaccgcg tcaggagggg 11160
cgacatccgc ggttgacgcg gcagcagatg gtgattacga acccccgcgg cgccgggccc 11220
ggcactacct ggacttggag gagggcgagg gcctggcgcg gctaggagcg ccctctcctg 11280
agcggcaccc aagggtgcag ctgaagcgtg atacgcgtga ggcgtacgtg ccgcggcaga 11340
acctgtttcg cgaccgcgag ggagaggagc ccgaggagat gcgggatcga aagttccacg 11400
cagggcgcga gctgcggcat ggcctgaatc gcgagcggtt gctgcgcgag gaggactttg 11460
agcccgacgc gcgaaccggg attagtcccg cgcgcgcaca cgtggcggcc gccgacctgg 11520
taaccgcata cgagcagacg gtgaaccagg agattaactt tcaaaaaagc tttaacaacc 11580
acgtgcgtac gcttgtggcg cgcgaggagg tggctatagg actgatgcat ctgtgggact 11640
ttgtaagcgc gctggagcaa aacccaaata gcaagccgct catggcgcag ctgttcctta 11700
tagtgcagca cagcagggac aacgaggcat tcagggatgc gctgctaaac atagtagagc 11760
ccgagggccg ctggctgctc gatttgataa acatcctgca gagcatagtg gtgcaggagc 11820
gcagcttgag cctggctgac aaggtggccg ccatcaacta ttccatgctt agcctgggca 11880
agttttacgc ccgcaagata taccataccc cttacgttcc catagacaag gaggtaaaga 11940
tcgaggggtt ctacatgcgc atggcgctga aggtgcttac cttgagcgac gacctgggcg 12000
tttatcgcaa cgagcgcatc cacaaggccg tgagcgtgag ccggcggcgc gagctcagcg 12060
accgcgagct gatgcacagc ctgcaaaggg ccctggctgg cacgggcagc ggcgatagag 12120
aggccgagtc ctactttgac gcgggcgctg acctgcgctg ggccccaagc cgacgcgccc 12180
tggaggcagc tggggccgga cctgggctgg cggtggcacc cgcgcgcgct ggcaacgtcg 12240
gcggcgtgga ggaatatgac gaggacgatg agtacgagcc agaggacggc gagtactaag 12300
cggtgatgtt tctgatcaga tgatgcaaga cgcaacggac ccggcggtgc gggcggcgct 12360
gcagagccag ccgtccggcc ttaactccac ggacgactgg cgccaggtca tggaccgcat 12420
catgtcgctg actgcgcgca atcctgacgc gttccggcag cagccgcagg ccaaccggct 12480
ctccgcaatt ctggaagcgg tggtcccggc gcgcgcaaac cccacgcacg agaaggtgct 12540
ggcgatcgta aacgcgctgg ccgaaaacag ggccatccgg cccgacgagg ccggcctggt 12600
ctacgacgcg ctgcttcagc gcgtggctcg ttacaacagc ggcaacgtgc agaccaacct 12660
ggaccggctg gtgggggatg tgcgcgaggc cgtggcgcag cgtgagcgcg cgcagcagca 12720
gggcaacctg ggctccatgg ttgcactaaa cgccttcctg agtacacagc ccgccaacgt 12780
gccgcgggga caggaggact acaccaactt tgtgagcgca ctgcggctaa tggtgactga 12840
gacaccgcaa agtgaggtgt accagtctgg gccagactat tttttccaga ccagtagaca 12900
aggcctgcag accgtaaacc tgagccaggc tttcaaaaac ttgcaggggc tgtggggggt 12960
gcgggctccc acaggcgacc gcgcgaccgt gtctagcttg ctgacgccca actcgcgcct 13020
gttgctgctg ctaatagcgc ccttcacgga cagtggcagc gtgtcccggg acacatacct 13080
aggtcacttg ctgacactgt accgcgaggc cataggtcag gcgcatgtgg acgagcatac 13140
tttccaggag attacaagtg tcagccgcgc gctggggcag gaggacacgg gcagcctgga 13200
ggcaacccta aactacctgc tgaccaaccg gcggcagaag atcccctcgt tgcacagttt 13260
aaacagcgag gaggagcgca ttttgcgcta cgtgcagcag agcgtgagcc ttaacctgat 13320
gcgcgacggg gtaacgccca gcgtggcgct ggacatgacc gcgcgcaaca tggaaccggg 13380
catgtatgcc tcaaaccggc cgtttatcaa ccgcctaatg gactacttgc atcgcgcggc 13440
cgccgtgaac cccgagtatt tcaccaatgc catcttgaac ccgcactggc taccgccccc 13500
tggtttctac accgggggat tcgaggtgcc cgagggtaac gatggattcc tctgggacga 13560
catagacgac agcgtgtttt ccccgcaacc gcagaccctg ctagagttgc aacagcgcga 13620
gcaggcagag gcggcgctgc gaaaggaaag cttccgcagg ccaagcagct tgtccgatct 13680
aggcgctgcg gccccgcggt cagatgctag tagcccattt ccaagcttga tagggtctct 13740
taccagcact cgcaccaccc gcccgcgcct gctgggcgag gaggagtacc taaacaactc 13800
gctgctgcag ccgcagcgcg aaaaaaacct gcctccggca tttcccaaca acgggataga 13860
gagcctagtg gacaagatga gtagatggaa gacgtacgcg caggagcaca gggacgtgcc 13920
aggcccgcgc ccgcccaccc gtcgtcaaag gcacgaccgt cagcggggtc tggtgtggga 13980
ggacgatgac tcggcagacg acagcagcgt cctggatttg ggagggagtg gcaacccgtt 14040
tgcgcacctt cgccccaggc tggggagaat gttttaaaaa aaaaaaaaaa gcatgatgca 14100
aaataaaaaa ctcaccaagg ccatggcacc gagcgttggt tttcttgtat tccccttagt 14160
atgcggcgcg cggcgatgta tgaggaaggt cctcctccct cctacgagag tgtggtgagc 14220
gcggcgccag tggcggcggc gctgggttct cccttcgatg ctcccctgga cccgccgttt 14280
gtgcctccgc ggtacctgcg gcctaccggg gggagaaaca gcatccgtta ctctgagttg 14340
gcacccctat tcgacaccac ccgtgtgtac ctggtggaca acaagtcaac ggatgtggca 14400
tccctgaact accagaacga ccacagcaac tttctgacca cggtcattca aaacaatgac 14460
tacagcccgg gggaggcaag cacacagacc atcaatcttg acgaccggtc gcactggggc 14520
ggcgacctga aaaccatcct gcataccaac atgccaaatg tgaacgagtt catgtttacc 14580
aataagttta aggcgcgggt gatggtgtcg cgcttgccta ctaaggacaa tcaggtggag 14640
ctgaaatacg agtgggtgga gttcacgctg cccgagggca actactccga gaccatgacc 14700
atagacctta tgaacaacgc gatcgtggag cactacttga aagtgggcag acagaacggg 14760
gttctggaaa gcgacatcgg ggtaaagttt gacacccgca acttcagact ggggtttgac 14820
cccgtcactg gtcttgtcat gcctggggta tatacaaacg aagccttcca tccagacatc 14880
attttgctgc caggatgcgg ggtggacttc acccacagcc gcctgagcaa cttgttgggc 14940
atccgcaagc ggcaaccctt ccaggagggc tttaggatca cctacgatga tctggagggt 15000
ggtaacattc ccgcactgtt ggatgtggac gcctaccagg cgagcttgaa agatgacacc 15060
gaacagggcg ggggtggcgc aggcggcagc aacagcagtg gcagcggcgc ggaagagaac 15120
tccaacgcgg cagccgcggc aatgcagccg gtggaggaca tgaacgatca tgccattcgc 15180
ggcgacacct ttgccacacg ggctgaggag aagcgcgctg aggccgaagc agcggccgaa 15240
gctgccgccc ccgctgcgca acccgaggtc gagaagcctc agaagaaacc ggtgatcaaa 15300
cccctgacag aggacagcaa gaaacgcagt tacaacctaa taagcaatga cagcaccttc 15360
acccagtacc gcagctggta ccttgcatac aactacggcg accctcagac cggaatccgc 15420
tcatggaccc tgctttgcac tcctgacgta acctgcggct cggagcaggt ctactggtcg 15480
ttgccagaca tgatgcaaga ccccgtgacc ttccgctcca cgcgccagat cagcaacttt 15540
ccggtggtgg gcgccgagct gttgcccgtg cactccaaga gcttctacaa cgaccaggcc 15600
gtctactccc aactcatccg ccagtttacc tctctgaccc acgtgttcaa tcgctttccc 15660
gagaaccaga ttttggcgcg cccgccagcc cccaccatca ccaccgtcag tgaaaacgtt 15720
cctgctctca cagatcacgg gacgctaccg ctgcgcaaca gcatcggagg agtccagcga 15780
gtgaccatta ctgacgccag acgccgcacc tgcccctacg tttacaaggc cctgggcata 15840
gtctcgccgc gcgtcctatc gagccgcact ttttgagcaa gcatgtccat ccttatatcg 15900
cccagcaata acacaggctg gggcctgcgc ttcccaagca agatgtttgg cggggccaag 15960
aagcgctccg accaacaccc agtgcgcgtg cgcgggcact accgcgcgcc ctggggcgcg 16020
cacaaacgcg gccgcactgg gcgcaccacc gtcgatgacg ccatcgacgc ggtggtggag 16080
gaggcgcgca actacacgcc cacgccgcca ccagtgtcca cagtggacgc ggccattcag 16140
accgtggtgc gcggagcccg gcgctatgct aaaatgaaga gacggcggag gcgcgtagca 16200
cgtcgccacc gccgccgacc cggcactgcc gcccaacgcg cggcggcggc cctgcttaac 16260
cgcgcacgtc gcaccggccg acgggcggcc atgcgggccg ctcgaaggct ggccgcgggt 16320
attgtcactg tgccccccag gtccaggcga cgagcggccg ccgcagcagc cgcggccatt 16380
agtgctatga ctcagggtcg caggggcaac gtgtattggg tgcgcgactc ggttagcggc 16440
ctgcgcgtgc ccgtgcgcac ccgccccccg cgcaactaga ttgcaagaaa aaactactta 16500
gactcgtact gttgtatgta tccagcggcg gcggcgcgca acgaagctat gtccaagcgc 16560
aaaatcaaag aagagatgct ccaggtcatc gcgccggaga tctatggccc cccgaagaag 16620
gaagagcagg attacaagcc ccgaaagcta aagcgggtca aaaagaaaaa gaaagatgat 16680
gatgatgaac ttgacgacga ggtggaactg ctgcacgcta ccgcgcccag gcgacgggta 16740
cagtggaaag gtcgacgcgt aaaacgtgtt ttgcgacccg gcaccaccgt agtctttacg 16800
cccggtgagc gctccacccg cacctacaag cgcgtgtatg atgaggtgta cggcgacgag 16860
gacctgcttg agcaggccaa cgagcgcctc ggggagtttg cctacggaaa gcggcataag 16920
gacatgctgg cgttgccgct ggacgagggc aacccaacac ctagcctaaa gcccgtaaca 16980
ctgcagcagg tgctgcccgc gcttgcaccg tccgaagaaa agcgcggcct aaagcgcgag 17040
tctggtgact tggcacccac cgtgcagctg atggtaccca agcgccagcg actggaagat 17100
gtcttggaaa aaatgaccgt ggaacctggg ctggagcccg aggtccgcgt gcggccaatc 17160
aagcaggtgg cgccgggact gggcgtgcag accgtggacg ttcagatacc cactaccagt 17220
agcaccagta ttgccaccgc cacagagggc atggagacac aaacgtcccc ggttgcctca 17280
gcggtggcgg atgccgcggt gcaggcggtc gctgcggccg cgtccaagac ctctacggag 17340
gtgcaaacgg acccgtggat gtttcgcgtt tcagcccccc ggcgcccgcg ccgttcgagg 17400
aagtacggcg ccgccagcgc gctactgccc gaatatgccc tacatccttc cattgcgcct 17460
acccccggct atcgtggcta cacctaccgc cccagaagac gagcaactac ccgacgccga 17520
accaccactg gaacccgccg ccgccgtcgc cgtcgccagc ccgtgctggc cccgatttcc 17580
gtgcgcaggg tggctcgcga aggaggcagg accctggtgc tgccaacagc gcgctaccac 17640
cccagcatcg tttaaaagcc ggtctttgtg gttcttgcag atatggccct cacctgccgc 17700
ctccgtttcc cggtgccggg attccgagga agaatgcacc gtaggagggg catggccggc 17760
cacggcctga cgggcggcat gcgtcgtgcg caccaccggc ggcggcgcgc gtcgcaccgt 17820
cgcatgcgcg gcggtatcct gcccctcctt attccactga tcgccgcggc gattggcgcc 17880
gtgcccggaa ttgcatccgt ggccttgcag gcgcagagac actgattaaa aacaagttgc 17940
atgtggaaaa atcaaaataa aaagtctgga ctctcacgct cgcttggtcc tgtaactatt 18000
ttgtagaatg gaagacatca actttgcgtc tctggccccg cgacacggct cgcgcccgtt 18060
catgggaaac tggcaagata tcggcaccag caatatgagc ggtggcgcct tcagctgggg 18120
ctcgctgtgg agcggcatta aaaatttcgg ttccaccgtt aagaactatg gcagcaaggc 18180
ctggaacagc agcacaggcc agatgctgag ggataagttg aaagagcaaa atttccaaca 18240
aaaggtggta gatggcctgg cctctggcat tagcggggtg gtggacctgg ccaaccaggc 18300
agtgcaaaat aagattaaca gtaagcttga tccccgccct cccgtagagg agcctccacc 18360
ggccgtggag acagtgtctc cagaggggcg tggcgaaaag cgtccgcgcc ccgacaggga 18420
agaaactctg gtgacgcaaa tagacgagcc tccctcgtac gaggaggcac taaagcaagg 18480
cctgcccacc acccgtccca tcgcgcccat ggctaccgga gtgctgggcc agcacacacc 18540
cgtaacgctg gacctgcctc cccccgccga cacccagcag aaacctgtgc tgccaggccc 18600
gaccgccgtt gttgtaaccc gtcctagccg cgcgtccctg cgccgcgccg ccagcggtcc 18660
gcgatcgttg cggcccgtag ccagtggcaa ctggcaaagc acactgaaca gcatcgtggg 18720
tctgggggtg caatccctga agcgccgacg atgcttctga tagctaacgt gtcgtatgtg 18780
tgtcatgtat gcgtccatgt cgccgccaga ggagctgctg agccgccgcg cgcccgcttt 18840
ccaagatggc taccccttcg atgatgccgc agtggtctta catgcacatc tcgggccagg 18900
acgcctcgga gtacctgagc cccgggctgg tgcagtttgc ccgcgccacc gagacgtact 18960
tcagcctgaa taacaagttt agaaacccca cggtggcgcc tacgcacgac gtgaccacag 19020
accggtccca gcgtttgacg ctgcggttca tccctgtgga ccgtgaggat actgcgtact 19080
cgtacaaggc gcggttcacc ctagctgtgg gtgataaccg tgtgctggac atggcttcca 19140
cgtactttga catccgcggc gtgctggaca ggggccctac ttttaagccc tactctggca 19200
ctgcctacaa cgccctggct cccaagggtg ccccaaatcc ttgcgaatgg gatgaagctg 19260
ctactgctct tgaaataaac ctagaagaag aggacgatga caacgaagac gaagtagacg 19320
agcaagctga gcagcaaaaa actcacgtat ttgggcaggc gccttattct ggtataaata 19380
ttacaaagga gggtattcaa ataggtgtcg aaggtcaaac acctaaatat gccgataaaa 19440
catttcaacc tgaacctcaa ataggagaat ctcagtggta cgaaacagaa attaatcatg 19500
cagctgggag agtcctaaaa aagactaccc caatgaaacc atgttacggt tcatatgcaa 19560
aacccacaaa tgaaaatgga gggcaaggca ttcttgtaaa gcaacaaaat ggaaagctag 19620
aaagtcaagt ggaaatgcaa tttttctcaa ctactgaggc agccgcaggc aatggtgata 19680
acttgactcc taaagtggta ttgtacagtg aagatgtaga tatagaaacc ccagacactc 19740
atatttctta catgcccact attaaggaag gtaactcacg agaactaatg ggccaacaat 19800
ctatgcccaa caggcctaat tacattgctt ttagggacaa ttttattggt ctaatgtatt 19860
acaacagcac gggtaatatg ggtgttctgg cgggccaagc atcgcagttg aatgctgttg 19920
tagatttgca agacagaaac acagagcttt cataccagct tttgcttgat tccattggtg 19980
atagaaccag gtacttttct atgtggaatc aggctgttga cagctatgat ccagatgtta 20040
gaattattga aaatcatgga actgaagatg aacttccaaa ttactgcttt ccactgggag 20100
gtgtgattaa tacagagact cttaccaagg taaaacctaa aacaggtcag gaaaatggat 20160
gggaaaaaga tgctacagaa ttttcagata aaaatgaaat aagagttgga aataattttg 20220
ccatggaaat caatctaaat gccaacctgt ggagaaattt cctgtactcc aacatagcgc 20280
tgtatttgcc cgacaagcta aagtacagtc cttccaacgt aaaaatttct gataacccaa 20340
acacctacga ctacatgaac aagcgagtgg tggctcccgg gctagtggac tgctacatta 20400
accttggagc acgctggtcc cttgactata tggacaacgt caacccattt aaccaccacc 20460
gcaatgctgg cctgcgctac cgctcaatgt tgctgggcaa tggtcgctat gtgcccttcc 20520
acatccaggt gcctcagaag ttctttgcca ttaaaaacct ccttctcctg ccgggctcat 20580
acacctacga gtggaacttc aggaaggatg ttaacatggt tctgcagagc tccctaggaa 20640
atgacctaag ggttgacgga gccagcatta agtttgatag catttgcctt tacgccacct 20700
tcttccccat ggcccacaac accgcctcca cgcttgaggc catgcttaga aacgacacca 20760
acgaccagtc ctttaacgac tatctctccg ccgccaacat gctctaccct atacccgcca 20820
acgctaccaa cgtgcccata tccatcccct cccgcaactg ggcggctttc cgcggctggg 20880
ccttcacgcg ccttaagact aaggaaaccc catcactggg ctcgggctac gacccttatt 20940
acacctactc tggctctata ccctacctag atggaacctt ttacctcaac cacaccttta 21000
agaaggtggc cattaccttt gactcttctg tcagctggcc tggcaatgac cgcctgctta 21060
cccccaacga gtttgaaatt aagcgctcag ttgacgggga gggttacaac gttgcccagt 21120
gtaacatgac caaagactgg ttcctggtac aaatgctagc taactataac attggctacc 21180
agggcttcta tatcccagag agctacaagg accgcatgta ctccttcttt agaaacttcc 21240
agcccatgag ccgtcaggtg gtggatgata ctaaatacaa ggactaccaa caggtgggca 21300
tcctacacca acacaacaac tctggatttg ttggctacct tgcccccacc atgcgcgaag 21360
gacaggccta ccctgctaac ttcccctatc cgcttatagg caagaccgca gttgacagca 21420
ttacccagaa aaagtttctt tgcgatcgca ccctttggcg catcccattc tccagtaact 21480
ttatgtccat gggcgcactc acagacctgg gccaaaacct tctctacgcc aactccgccc 21540
acgcgctaga catgactttt gaggtggatc ccatggacga gcccaccctt ctttatgttt 21600
tgtttgaagt ctttgacgtg gtccgtgtgc accagccgca ccgcggcgtc atcgaaaccg 21660
tgtacctgcg cacgcccttc tcggccggca acgccacaac ataaagaagc aagcaacatc 21720
aacaacagct gccgccatgg gctccagtga gcaggaactg aaagccattg tcaaagatct 21780
tggttgtggg ccatattttt tgggcaccta tgacaagcgc tttccaggct ttgtttctcc 21840
acacaagctc gcctgcgcca tagtcaatac ggccggtcgc gagactgggg gcgtacactg 21900
gatggccttt gcctggaacc cgcactcaaa aacatgctac ctctttgagc cctttggctt 21960
ttctgaccag cgactcaagc aggtttacca gtttgagtac gagtcactcc tgcgccgtag 22020
cgccattgct tcttcccccg accgctgtat aacgctggaa aagtccaccc aaagcgtaca 22080
ggggcccaac tcggccgcct gtggactatt ctgctgcatg tttctccacg cctttgccaa 22140
ctggccccaa actcccatgg atcacaaccc caccatgaac cttattaccg gggtacccaa 22200
ctccatgctc aacagtcccc aggtacagcc caccctgcgt cgcaaccagg aacagctcta 22260
cagcttcctg gagcgccact cgccctactt ccgcagccac agtgcgcaga ttaggagcgc 22320
cacttctttt tgtcacttga aaaacatgta aaaataatgt actagagaca ctttcaataa 22380
aggcaaatgc ttttatttgt acactctcgg gtgattattt acccccaccc ttgccgtctg 22440
cgccgtttaa aaatcaaagg ggttctgccg cgcatcgcta tgcgccactg gcagggacac 22500
gttgcgatac tggtgtttag tgctccactt aaactcaggc acaaccatcc gcggcagctc 22560
ggtgaagttt tcactccaca ggctgcgcac catcaccaac gcgtttagca ggtcgggcgc 22620
cgatatcttg aagtcgcagt tggggcctcc gccctgcgcg cgcgagttgc gatacacagg 22680
gttgcagcac tggaacacta tcagcgccgg gtggtgcacg ctggccagca cgctcttgtc 22740
ggagatcaga tccgcgtcca ggtcctccgc gttgctcagg gcgaacggag tcaactttgg 22800
tagctgcctt cccaaaaagg gcgcgtgccc aggctttgag ttgcactcgc accgtagtgg 22860
catcaaaagg tgaccgtgcc cggtctgggc gttaggatac agcgcctgca taaaagcctt 22920
gatctgctta aaagccacct gagcctttgc gccttcagag aagaacatgc cgcaagactt 22980
gccggaaaac tgattggccg gacaggccgc gtcgtgcacg cagcaccttg cgtcggtgtt 23040
ggagatctgc accacatttc ggccccaccg gttcttcacg atcttggcct tgctagactg 23100
ctccttcagc gcgcgctgcc cgttttcgct cgtcacatcc atttcaatca cgtgctcctt 23160
atttatcata atgcttccgt gtagacactt aagctcgcct tcgatctcag cgcagcggtg 23220
cagccacaac gcgcagcccg tgggctcgtg atgcttgtag gtcacctctg caaacgactg 23280
caggtacgcc tgcaggaatc gccccatcat cgtcacaaag gtcttgttgc tggtgaaggt 23340
cagctgcaac ccgcggtgct cctcgttcag ccaggtcttg catacggccg ccagagcttc 23400
cacttggtca ggcagtagtt tgaagttcgc ctttagatcg ttatccacgt ggtacttgtc 23460
catcagcgcg cgcgcagcct ccatgccctt ctcccacgca gacacgatcg gcacactcag 23520
cgggttcatc accgtaattt cactttccgc ttcgctgggc tcttcctctt cctcttgcgt 23580
ccgcatacca cgcgccactg ggtcgtcttc attcagccgc cgcactgtgc gcttacctcc 23640
tttgccatgc ttgattagca ccggtgggtt gctgaaaccc accatttgta gcgccacatc 23700
ttctctttct tcctcgctgt ccacgattac ctctggtgat ggcgggcgct cgggcttggg 23760
agaagggcgc ttctttttct tcttgggcgc aatggccaaa tccgccgccg aggtcgatgg 23820
ccgcgggctg ggtgtgcgcg gcaccagcgc gtcttgtgat gagtcttcct cgtcctcgga 23880
ctcgatacgc cgcctcatcc gcttttttgg gggcgcccgg ggaggcggcg gcgacgggga 23940
cggggacgac acgtcctcca tggttggggg acgtcgcgcc gcaccgcgtc cgcgctcggg 24000
ggtggtttcg cgctgctcct cttcccgact ggccatttcc ttctcctata ggcagaaaaa 24060
gatcatggag tcagtcgaga agaaggacag cctaaccgcc ccctctgagt tcgccaccac 24120
cgcctccacc gatgccgcca acgcgcctac caccttcccc gtcgaggcac ccccgcttga 24180
ggaggaggaa gtgattatcg agcaggaccc aggttttgta agcgaagacg acgaggaccg 24240
ctcagtacca acagaggata aaaagcaaga ccaggacaac gcagaggcaa acgaggaaca 24300
agtcgggcgg ggggacgaaa ggcatggcga ctacctagat gtgggagacg acgtgctgtt 24360
gaagcatctg cagcgccagt gcgccattat ctgcgacgcg ttgcaagagc gcagcgatgt 24420
gcccctcgcc atagcggatg tcagccttgc ctacgaacgc cacctattct caccgcgcgt 24480
accccccaaa cgccaagaaa acggcacatg cgagcccaac ccgcgcctca acttctaccc 24540
cgtatttgcc gtgccagagg tgcttgccac ctatcacatc tttttccaaa actgcaagat 24600
acccctatcc tgccgtgcca accgcagccg agcggacaag cagctggcct tgcggcaggg 24660
cgctgtcata cctgatatcg cctcgctcaa cgaagtgcca aaaatctttg agggtcttgg 24720
acgcgacgag aagcgcgcgg caaacgctct gcaacaggaa aacagcgaaa atgaaagtca 24780
ctctggagtg ttggtggaac tcgagggtga caacgcgcgc ctagccgtac taaaacgcag 24840
catcgaggtc acccactttg cctacccggc acttaaccta ccccccaagg tcatgagcac 24900
agtcatgagt gagctgatcg tgcgccgtgc gcagcccctg gagagggatg caaatttgca 24960
agaacaaaca gaggagggcc tacccgcagt tggcgacgag cagctagcgc gctggcttca 25020
aacgcgcgag cctgccgact tggaggagcg acgcaaacta atgatggccg cagtgctcgt 25080
taccgtggag cttgagtgca tgcagcggtt ctttgctgac ccggagatgc agcgcaagct 25140
agaggaaaca ttgcactaca cctttcgaca gggctacgta cgccaggcct gcaagatctc 25200
caacgtggag ctctgcaacc tggtctccta ccttggaatt ttgcacgaaa accgccttgg 25260
gcaaaacgtg cttcattcca cgctcaaggg cgaggcgcgc cgcgactacg tccgcgactg 25320
cgtttactta tttctatgct acacctggca gacggccatg ggcgtttggc agcagtgctt 25380
ggaggagtgc aacctcaagg agctgcagaa actgctaaag caaaacttga aggacctatg 25440
gacggccttc aacgagcgct ccgtggccgc gcacctggcg gacatcattt tccccgaacg 25500
cctgcttaaa accctgcaac agggtctgcc agacttcacc agtcaaagca tgttgcagaa 25560
ctttaggaac tttatcctag agcgctcagg aatcttgccc gccacctgct gtgcacttcc 25620
tagcgacttt gtgcccatta agtaccgcga atgccctccg ccgctttggg gccactgcta 25680
ccttctgcag ctagccaact accttgccta ccactctgac ataatggaag acgtgagcgg 25740
tgacggtcta ctggagtgtc actgtcgctg caacctatgc accccgcacc gctccctggt 25800
ttgcaattcg cagctgctta acgaaagtca aattatcggt acctttgagc tgcagggtcc 25860
ctcgcctgac gaaaagtccg cggctccggg gttgaaactc actccggggc tgtggacgtc 25920
ggcttacctt cgcaaatttg tacctgagga ctaccacgcc cacgagatta ggttctacga 25980
agaccaatcc cgcccgccta atgcggagct taccgcctgc gtcattaccc agggccacat 26040
tcttggccaa ttgcaagcca tcaacaaagc ccgccaagag tttctgctac gaaagggacg 26100
gggggtttac ttggaccccc agtccggcga ggagctcaac ccaatccccc cgccgccgca 26160
gccctatcag cagcagccgc gggcccttgc ttcccaggat ggcacccaaa aagaagctgc 26220
agctgccgcc gccacccacg gacgaggagg aatactggga cagtcaggca gaggaggttt 26280
tggacgagga ggaggaggac atgatggaag actgggagag cctagacgag gaagcttccg 26340
aggtcgaaga ggtgtcagac gaaacaccgt caccctcggt cgcattcccc tcgccggcgc 26400
cccagaaatc ggcaaccggt tccagcatgg ctacaacctc cgctcctcag gcgccgccgg 26460
cactgcccgt tcgccgaccc aaccgtagat gggacaccac tggaaccagg gccggtaagt 26520
ccaagcagcc gccgccgtta gcccaagagc aacaacagcg ccaaggctac cgctcatggc 26580
gcgggcacaa gaacgccata gttgcttgct tgcaagactg tgggggcaac atctccttcg 26640
cccgccgctt tcttctctac catcacggcg tggccttccc ccgtaacatc ctgcattact 26700
accgtcatct ctacagccca tactgcaccg gcggcagcgg cagcaacagc agcggccaca 26760
cagaagcaaa ggcgaccgga tagcaagact ctgacaaagc ccaagaaatc cacagcggcg 26820
gcagcagcag gaggaggagc gctgcgtctg gcgcccaacg aacccgtatc gacccgcgag 26880
cttagaaaca ggatttttcc cactctgtat gctatatttc aacagagcag gggccaagaa 26940
caagagctga aaataaaaaa caggtctctg cgatccctca cccgcagctg cctgtatcac 27000
aaaagcgaag atcagcttcg gcgcacgctg gaagacgcgg aggctctctt cagtaaatac 27060
tgcgcgctga ctcttaagga ctagtttcgc gccctttctc aaatttaagc gcgaaaacta 27120
cgtcatctcc agcggccaca cccggcgcca gcacctgtcg tcagcgccat ttatgagcaa 27180
ggaaattccc acgccctaca tgtggagtta ccagccacaa atgggacttg cggctggagc 27240
tgcccaagac tactcaaccc gaataaacta catgagcgcg ggaccccaca tgatatcccg 27300
ggtcaacgga atacgcgccc accgaaaccg aattctcctg gaacaggcgg ctattaccac 27360
cacacctcgt aataacctta atccccgtag ttggcccgct gccctggtgt accaggaaag 27420
tcccgctccc accactgtgg tacttcccag agacgcccag gccgaagttc agatgactaa 27480
ctcaggggcg cagcttgcgg gcggctttcg tcacagggtg cggtcgcccg ggcagggtat 27540
aactcacctg acaatcagag ggcgaggtat tcagctcaac gacgagtcgg tgagctcctc 27600
gcttggtctc cgtccggacg ggacatttca gatcggcggc gccggccgct cttcattcac 27660
gcctcgtcag gcaatcctaa ctctgcagac ctcgtcctct gagccgcgct ctggaggcat 27720
tggaactctg caatttattg aggagtttgt gccatcggtc tactttaacc ccttctcggg 27780
acctcccggc cactatccgg atcaatttat tcctaacttt gacgcggtaa aggactcggc 27840
ggacggctac gactgaatgt taagtggaga ggcagagcaa ctgcgcctga aacacctggt 27900
ccactgtcgc cgccacaagt gctttgcccg cgactccggt gagttttgct actttgaatt 27960
gcccgaggat catatcgagg gcccggcgca cggcgtccgg cttaccgccc agggagagct 28020
tgcccgtagc ctgattcggg agtttaccca gcgccccctg ctagttgagc gggacagggg 28080
accctgtgtt ctcactgtga tttgcaactg tcctaaccct ggattacatc aagatctttg 28140
ttgccatctc tgtgctgagt ataataaata cagaaattaa aatatactgg ggctcctatc 28200
gccatcctgt aaacgccacc gtcttcaccc gcccaagcaa accaaggcga accttacctg 28260
gtacttttaa catctctccc tctgtgattt acaacagttt caacccagac ggagtgagtc 28320
tacgagagaa cctctccgag ctcagctact ccatcagaaa aaacaccacc ctccttacct 28380
gccgggaacg tacgagtgcg tcaccggccg ctgcaccaca cctaccgcct gaccgtaaac 28440
cagacttttt ccggacagac ctcaataact ctgtttacca gaacaggagg tgagcttaga 28500
aaacccttag ggtattaggc caaaggcgca gctactgtgg ggtttatgaa caattcaagc 28560
aactctacgg gctattctaa ttcaggtttc tctagaatcg gggttggggt tattctctgt 28620
cttgtgattc tctttattct tatactaacg cttctctgcc taaggctcgc cgcctgctgt 28680
gtgcacattt gcatttattg tcagcttttt aaacgctggg gtcgccaccc aagatgatta 28740
ggtacataat cctaggttta ctcacccttg cgtcagccca cggtaccacc caaaaggtgg 28800
attttaagga gccagcctgt aatgttacat tcgcagctga agctaatgag tgcaccactc 28860
ttataaaatg caccacagaa catgaaaagc tgcttattcg ccacaaaaac aaaattggca 28920
agtatgctgt ttatgctatt tggcagccag gtgacactac agagtataat gttacagttt 28980
tccagggtaa aagtcataaa acttttatgt atacttttcc attttatgaa atgtgcgaca 29040
ttaccatgta catgagcaaa cagtataagt tgtggccccc acaaaattgt gtggaaaaca 29100
ctggcacttt ctgctgcact gctatgctaa ttacagtgct cgctttggtc tgtaccctac 29160
tctatattaa atacaaaagc agacgcagct ttattgagga aaagaaaatg ccttaattta 29220
ctaagttaca aagctaatgt caccactaac tgctttactc gctgcttgca aaacaaattc 29280
aaaaagttag cattataatt agaataggat ttaaaccccc cggtcatttc ctgctcaata 29340
ccattcccct gaacaattga ctctatgtgg gatatgctcc agcgctacaa ccttgaagtc 29400
aggcttcctg gatgtcagca tctgactttg gccagcacct gtcccgcgga tttgttccag 29460
tccaactaca gcgacccacc ctaacagaga tgaccaacac aaccaacgcg gccgccgcta 29520
ccggacttac atctaccaca aatacacccc aagtttctgc ctttgtcaat aactgggata 29580
acttgggcat gtggtggttc tccatagcgc ttatgtttgt atgccttatt attatgtggc 29640
tcatctgctg cctaaagcgc aaacgcgccc gaccacccat ctatagtccc atcattgtgc 29700
tacacccaaa caatgatgga atccatagat tggacggact gaaacacatg ttcttttctc 29760
ttacagtatg attaaatgag acatgattcc tcgagttttt atattactga cccttgttgc 29820
gcttttttgt gcgtgctcca cattggctgc ggtttctcac atcgaagtag actgcattcc 29880
agccttcaca gtctatttgc tttacggatt tgtcaccctc acgctcatct gcagcctcat 29940
cactgtggtc atcgccttta tccagtgcat tgactgggtc tgtgtgcgct ttgcatatct 30000
cagacaccat ccccagtaca gggacaggac tatagctgag cttcttagaa ttctttaatt 30060
atgaaattta ctgtgacttt tctgctgatt atttgcaccc tatctgcgtt ttgttccccg 30120
acctccaagc ctcaaagaca tatatcatgc agattcactc gtatatggaa tattccaagt 30180
tgctacaatg aaaaaagcga tctttccgaa gcctggttat atgcaatcat ctctgttatg 30240
gtgttctgca gtaccatctt agccctagct atatatccct accttgacat tggctggaac 30300
gcaatagatg ccatgaacca cccaactttc cccgcgcccg ctatgcttcc actgcaacaa 30360
gttgttgccg gcggctttgt cccagccaat cagcctcgcc caccttctcc cacccccact 30420
gaaatcagct actttaatct aacaggagga gatgactgac accctagatc tagaaatgga 30480
cggaattatt acagagcagc gcctgctaga aagacgcagg gcagcggccg agcaacagcg 30540
catgaatcaa gagctccaag acatggttaa cttgcaccag tgcaaaaggg gtatcttttg 30600
tctggtaaag caggccaaag tcacctacga cagtaatacc accggacacc gccttagcta 30660
caagttgcca accaagcgtc agaaattggt ggtcatggtg ggagaaaagc ccattaccat 30720
aactcagcac tcggtagaaa ccgaaggctg cattcactca ccttgtcaag gacctgagga 30780
tctctgcacc cttattaaga ccctgtgcgg tctcaaagat cttattccct ttaacggaag 30840
cggagctact aacttcagcc tgctgaagca ggctggtgac gtcgaggaga atcctggccc 30900
aatggtgagc aaaggcgaag agctgttcac cggcgtggtg cccatcctgg tggagctgga 30960
cggcgacgtg aacggccaca agttcagcgt gagcggcgag ggcgagggcg acgccaccta 31020
cggcaagctg accctgaagc tgctgtgcac caccggcaag ctgcccgtgc cctggcccac 31080
cctggtgacc accctgggct acggcgtgca gtgcttcgcc cggtaccccg accacatgaa 31140
gcagcacgac ttcttcaaga gcgccatgcc cgagggctac gtgcaggagc ggaccatctt 31200
cttcaaggac gacggcaact acaagacccg ggccgaggtg aagttcgagg gcgacaccct 31260
ggtgaaccgg atcgagctga agggcatcga cttcaaggag gacggcaaca tcctgggcca 31320
caagctggag tacaactaca acagccacaa cgtgtacatc accgccgaca agcagaagaa 31380
cggcatcaag gccaacttca agatccggca caacatcgag gacggcggcg tgcagctggc 31440
cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 31500
ctacctgagc taccagagcg ccctgttcaa ggaccccaac gagaagcggg accacatggt 31560
gctgctggag ttcctgaccg ccgccggcat caccgagggc atgaacgagc tctataagta 31620
ataaaaaaaa ataataaagc atcacttact taaaatcagt tagcaaattt ctgtccagtt 31680
tattcagcag cacctccttg ccctcctccc agctctggta ttgcagcttc ctcctggctg 31740
caaactttct ccacaatcta aatggaatgt cagtttcctc ctgttcctgt ccatccgcac 31800
ccactatctt catgttgttg cagatgaagc gcgcaagacc gtctgaagat accttcaacc 31860
ccgtgtatcc atatgacacg gaaaccggtc ctccaactgt gccttttctt actcctccct 31920
ttgtatcccc caatgggttt caagagagtc cccctggggt actctctttg cgcctatccg 31980
aacctctagt tacctccaat ggcatgcttg cgctcaaaat gggcaacggc ctctctctgg 32040
acgaggccgg caaccttacc tcccaaaatg taaccactgt gagcccacct ctcaaaaaaa 32100
ccaagtcaaa cataaacctg gaaatatctg cacccctcac agttacctca gaagccctaa 32160
ctgtggctgc cgccgcacct ctaatggtcg cgggcaacac actcaccatg caatcacagg 32220
ccccgctaac cgtgcacgac tccaaactta gcattgccac ccaaggaccc ctcacagtgt 32280
cagaaggaaa gctagccctg caaacatcag gccccctcac caccaccgat agcagtaccc 32340
ttactatcac tgcctcaccc cctctaacta ctgccactgg tagcttgggc attgacttga 32400
aagagcccat ttatacacaa aatggaaaac taggactaaa gtacggggct cctttgcatg 32460
taacagacga cctaaacact ttgaccgtag caactggtcc aggtgtgact attaataata 32520
cttccttgca aactaaagtt actggagcct tgggttttga ttcacaaggc aatatgcaac 32580
ttaatgtagc aggaggacta aggattgatt ctcaaaacag acgccttata cttgatgtta 32640
gttatccgtt tgatgctcaa aaccaactaa atctaagact aggacagggc cctcttttta 32700
taaactcagc ccacaacttg gatattaact acaacaaagg cctttacttg tttacagctt 32760
caaacaattc caaaaagctt gaggttaacc taagcactgc caaggggttg atgtttgacg 32820
ctacagccat agccattaat gcaggagatg ggcttgaatt tggttcacct aatgcaccaa 32880
acacaaatcc cctcaaaaca aaaattggcc atggcctaga atttgattca aacaaggcta 32940
tggttcctaa actaggaact ggccttagtt ttgacagcac aggtgccatt acagtaggaa 33000
acaaaaataa tgataagcta actttgtgga ccacaccagc tccatctcct aactgtagac 33060
taaatgcaga gaaagatgct aaactcactt tggtcttaac aaaatgtggc agtcaaatac 33120
ttgctacagt ttcagttttg gctgttaaag gcagtttggc tccaatatct ggaacagttc 33180
aaagtgctca tcttattata agatttgacg aaaatggagt gctactaaac aattccttcc 33240
tggacccaga atattggaac tttagaaatg gagatcttac tgaaggcaca gcctatacaa 33300
acgctgttgg atttatgcct aacctatcag cttatccaaa atctcacggt aaaactgcca 33360
aaagtaacat tgtcagtcaa gtttacttaa acggagacaa aactaaacct gtaacactaa 33420
ccattacact aaacggtaca caggaaacag gagacacaac tccaagtgca tactctatgt 33480
cattttcatg ggactggtct ggccacaact acattaatga aatatttgcc acatcctctt 33540
acactttttc atacattgcc caagaataaa gaatcgtttg tgttatgttt caacgtgttt 33600
atttttcaat tgcagaaaat ttcgaatcat ttttcattca gtagtatagc cccaccacca 33660
catagcttat acagatcacc gtacctaaat caaactcaca gaaccctagt attcaacctg 33720
ccacctccct cccaacacac agagtacaca gtcctttctc cccggctggc cttaaaaagc 33780
atcatatcat gggtaacaga catattctta ggtgttatat tccacacggt ttcctgtcga 33840
gccaaacgct catcagtgat attaataaac tccccgggca gctcacttaa gttcatgtcg 33900
ctgtccagct gctgagccac aggctgctgt ccaacttgcg gttgcttaac gggcggcgaa 33960
ggagaagtcc acgcctacat gggggtagag tcataatcgt gcatcaggat agggcggtgg 34020
tgctgcagca gcgcgcgaat aaactgctgc cgccgccgct ccgtcctgca ggaatacaac 34080
atggcagtgg tctcctcagc gatgattcgc accgcccgca gcataaggcg ccttgtcctc 34140
cgggcacagc agcgcaccct gatctcactt aaatcagcac agtaactgca gcacagcacc 34200
acaatattgt tcaaaatccc acagtgcaag gcgctgtatc caaagctcat ggcggggacc 34260
acagaaccca cgtggccatc ataccacaag cgcaggtaga ttaagtggcg acccctcata 34320
aacacgctgg acataaacat tacctctttt ggcatgttgt aattcaccac ctcccggtac 34380
catataaacc tctgattaaa catggcgcca tccaccacca tcctaaacca gctggccaaa 34440
acctgcccgc cggctataca ctgcagggaa ccgggactgg aacaatgaca gtggagagcc 34500
caggactcgt aaccatggat catcatgctc gtcatgatat caatgttggc acaacacagg 34560
cacacgtgca tacacttcct caggattaca agctcctccc gcgttagaac catatcccag 34620
ggaacaaccc attcctgaat cagcgtaaat cccacactgc agggaagacc tcgcacgtaa 34680
ctcacgttgt gcattgtcaa agtgttacat tcgggcagca gcggatgatc ctccagtatg 34740
gtagcgcggg tttctgtctc aaaaggaggt agacgatccc tactgtacgg agtgcgccga 34800
gacaaccgag atcgtgttgg tcgtagtgtc atgccaaatg gaacgccgga cgtagtcata 34860
tttcctgaag caaaaccagg tgcgggcgtg acaaacagat ctgcgtctcc ggtctcgccg 34920
cttagatcgc tctgtgtagt agttgtagta tatccactct ctcaaagcat ccaggcgccc 34980
cctggcttcg ggttctatgt aaactccttc atgcgccgct gccctgataa catccaccac 35040
cgcagaataa gccacaccca gccaacctac acattcgttc tgcgagtcac acacgggagg 35100
agcgggaaga gctggaagaa ccatgttttt ttttttattc caaaagatta tccaaaacct 35160
caaaatgaag atctattaag tgaacgcgct cccctccggt ggcgtggtca aactctacag 35220
ccaaagaaca gataatggca tttgtaagat gttgcacaat ggcttccaaa aggcaaacgg 35280
ccctcacgtc caagtggacg taaaggctaa acccttcagg gtgaatctcc tctataaaca 35340
ttccagcacc ttcaaccatg cccaaataat tctcatctcg ccaccttctc aatatatctc 35400
taagcaaatc ccgaatatta agtccggcca ttgtaaaaat ctgctccaga gcgccctcca 35460
ccttcagcct caagcagcga atcatgattg caaaaattca ggttcctcac agacctgtat 35520
aagattcaaa agcggaacat taacaaaaat accgcgatcc cgtaggtccc ttcgcagggc 35580
cagctgaaca taatcgtgca ggtctgcacg gaccagcgcg gccacttccc cgccaggaac 35640
catgacaaaa gaacccacac tgattatgac acgcatactc ggagctatgc taaccagcgt 35700
agccccgatg taagcttgtt gcatgggcgg cgatataaaa tgcaaggtgc tgctcaaaaa 35760
atcaggcaaa gcctcgcgca aaaaagaaag cacatcgtag tcatgctcat gcagataaag 35820
gcaggtaagc tccggaacca ccacagaaaa agacaccatt tttctctcaa acatgtctgc 35880
gggtttctgc ataaacacaa aataaaataa caaaaaaaca tttaaacatt agaagcctgt 35940
cttacaacag gaaaaacaac ccttataagc ataagacgga ctacggccat gccggcgtga 36000
ccgtaaaaaa actggtcacc gtgattaaaa agcaccaccg acagctcctc ggtcatgtcc 36060
ggagtcataa tgtaagactc ggtaaacaca tcaggttgat tcacatcggt cagtgctaaa 36120
aagcgaccga aatagcccgg gggaatacat acccgcaggc gtagagacaa cattacagcc 36180
cccataggag gtataacaaa attaatagga gagaaaaaca cataaacacc tgaaaaaccc 36240
tcctgcctag gcaaaatagc accctcccgc tccagaacaa catacagcgc ttccacagcg 36300
gcagccataa cagtcagcct taccagtaaa aaagaaaacc tattaaaaaa acaccactcg 36360
acacggcacc agctcaatca gtcacagtgt aaaaaagggc caagtgcaga gcgagtatat 36420
ataggactaa aaaatgacgt aacggttaaa gtccacaaaa aacacccaga aaaccgcacg 36480
cgaacctacg cccagaaacg aaagccaaaa aacccacaac ttcctcaaat cgtcacttcc 36540
gttttcccac gttacgtcac ttcccatttt aagaaaacta caattcccaa cacatacaag 36600
ttactccgcc ctaaaaccta cgtcacccgc cccgttccca cgccccgcgc cacgtcacaa 36660
actccacccc ctcattatca tattggcttc aatccaaaat aaggtatatt attgatgatg 36720
<210> 10
<211> 36790
<212> DNA
<213> 人工序列
<220>
<223> 合成腺病毒基因组YPet-P2A-E4-ORF2
<400> 10
catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60
ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120
gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180
gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240
taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300
agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360
gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420
cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480
tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540
tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600
aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660
tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720
cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780
gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840
cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900
ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960
cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020
caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080
ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140
tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200
gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260
ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320
cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380
ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440
gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500
cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560
ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620
gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680
cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740
ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800
tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860
gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920
caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980
gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040
agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100
aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160
cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220
gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280
gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340
gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400
gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460
tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520
ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580
tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640
agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700
tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760
gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820
acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880
gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940
aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000
ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060
gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120
tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180
acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240
aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300
tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360
gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420
tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480
ttggctctag cgatgaagat acagattgag gtactgaaat gtgtgggcgt ggcttaaggg 3540
tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg 3600
ccgccgccca acttttctat acaaagttgt aatgagcacc aactcgtttg atggaagcat 3660
tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat 3720
gggctccagc attgatggtc gccccgtcct gcccgcaaac tctactacct tgacctacga 3780
gaccgtgtct ggaacgccgt tggagactgc agcctccgcc gccgcttcag ccgctgcagc 3840
caccgcccgc gggattgtga ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc 3900
ttcccgttca tccgcccgcg atgacaagtt gacggctctt ttggcacaat tggattcttt 3960
gacccgggaa cttaatgtcg tttctcagca gctgttggat ctgcgccagc aggtttctgc 4020
cctgaaggct tcctcccctc ccaatgcggt ttaaaacata aataaaaaac cagactctgt 4080
ttggatttgg atcaagcata agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg 4140
taggcccggg accagcggtc tcggtcgttg agggtcctgt gtattttttc caggacgtgg 4200
taaaggtgac tctggatgtt cagatacatg ggcataagcc cgtctctggg gtggaggtag 4260
caccactgca gagcttcatg ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag 4320
cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc tgattgccag gggcaggccc 4380
ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt gcatacgtgg ggatatgaga 4440
tgcatcttgg actgtatttt taggttggct atgttcccag ccatatccct ccggggattc 4500
atgttgtgca gaaccaccag cacagtgtat ccggtgcact tgggaaattt gtcatgtagc 4560
ttagaaggaa atgcgtggaa gaacttggag acgcccttgt gacctccaag attttccatg 4620
cattcgtcca taatgatggc aatgggccca cgggcggcgg cctgggcgaa gatatttctg 4680
ggatcactaa cgtcatagtt gtgttccagg atgagatcgt cataggccat ttttacaaag 4740
cgcgggcgga gggtgccaga ctgcggtata atggttccat ccggcccagg ggcgtagtta 4800
ccctcacaga tttgcatttc ccacgctttg agttcagatg gggggatcat gtctacctgc 4860
ggggcgatga agaaaacggt ttccggggta ggggagatca gctgggaaga aagcaggttc 4920
ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa tcacacctat taccgggtgc 4980
aactggtagt taagagagct gcagctgccg tcatccctga gcaggggggc cacttcgtta 5040
agcatgtccc tgactcgcat gttttccctg accaaatccg ccagaaggcg ctcgccgccc 5100
agcgatagca gttcttgcaa ggaagcaaag tttttcaacg gtttgagacc gtccgccgta 5160
ggcatgcttt tgagcgtttg accaagcagt tccaggcggt cccacagctc ggtcacctgc 5220
tctacggcat ctcgatccag catatctcct cgtttcgcgg gttggggcgg ctttcgctgt 5280
acggcagtag tcggtgctcg tccagacggg ccagggtcat gtctttccac gggcgcaggg 5340
tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc tccgggctgc gcgctggcca 5400
gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt 5460
cggccaggta gcatttgacc atggtgtcat agtccagccc ctccgcggcg tggcccttgg 5520
cgcgcagctt gcccttggag gaggcgccgc acgaggggca gtgcagactt ttgagggcgt 5580
agagcttggg cgcgagaaat accgattccg gggagtaggc atccgcgccg caggccccgc 5640
agacggtctc gcattccacg agccaggtga gctctggccg ttcggggtca aaaaccaggt 5700
ttcccccatg ctttttgatg cgtttcttac ctctggtttc catgagccgg tgtccacgct 5760
cggtgacgaa aaggctgtcc gtgtccccgt atacagactt gagaggcctg tcctcgagcg 5820
gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacaaag gctcgcgtcc 5880
aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact agggggtcca 5940
ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag gtgattggtt 6000
tgtaggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag ggggtggggg 6060
cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt tggggtgagt 6120
actccctctg aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg 6180
aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgca tccatctggt 6240
cagaaaagac aatctttttg ttgtcaagct tggtggcaaa cgacccgtag agggcgttgg 6300
acagcaactt ggcgatggag cgcagggttt ggtttttgtc gcgatcggcg cgctccttgg 6360
ccgcgatgtt tagctgcacg tattcgcgcg caacgcaccg ccattcggga aagacggtgg 6420
tgcgctcgtc gggcaccagg tgcacgcgcc aaccgcggtt gtgcagggtg acaaggtcaa 6480
cgctggtggc tacctctccg cgtaggcgct cgttggtcca gcagaggcgg ccgcccttgc 6540
gcgagcagaa tggcggtagg gggtctagct gcgtctcgtc cggggggtct gcgtccacgg 6600
taaagacccc gggcagcagg cgcgcgtcga agtagtctat cttgcatcct tgcaagtcta 6660
gcgcctgctg ccatgcgcgg gcggcaagcg cgcgctcgta tgggttgagt gggggacccc 6720
atggcatggg gtgggtgagc gcggaggcgt acatgccgca aatgtcgtaa acgtagaggg 6780
gctctctgag tattccaaga tatgtagggt agcatcttcc accgcggatg ctggcgcgca 6840
cgtaatcgta tagttcgtgc gagggagcga ggaggtcggg accgaggttg ctacgggcgg 6900
gctgctctgc tcggaagact atctgcctga agatggcatg tgagttggat gatatggttg 6960
gacgctggaa gacgttgaag ctggcgtctg tgagacctac cgcgtcacgc acgaaggagg 7020
cgtaggagtc gcgcagcttg ttgaccagct cggcggtgac ctgcacgtct agggcgcagt 7080
agtccagggt ttccttgatg atgtcatact tatcctgtcc cttttttttc cacagctcgc 7140
ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct 7200
ccgaacggta agagcctagc atgtagaact ggttgacggc ctggtaggcg cagcatccct 7260
tttctacggg tagcgcgtat gcctgcgcgg ccttccggag cgaggtgtgg gtgagcgcaa 7320
aggtgtccct gaccatgact ttgaggtact ggtatttgaa gtcagtgtcg tcgcatccgc 7380
cctgctccca gagcaaaaag tccgtgcgct ttttggaacg cggatttggc agggcgaagg 7440
tgacatcgtt gaagagtatc tttcccgcgc gaggcataaa gttgcgtgtg atgcggaagg 7500
gtcccggcac ctcggaacgg ttgttaatta cctgggcggc gagcacgatc tcgtcaaagc 7560
cgttgatgtt gtggcccaca atgtaaagtt ccaagaagcg cgggatgccc ttgatggaag 7620
gcaatttttt aagttcctcg taggtgagct cttcagggga gctgagcccg tgctctgaaa 7680
gggcccagtc tgcaagatga gggttggaag cgacgaatga gctccacagg tcacgggcca 7740
ttagcatttg caggtggtcg cgaaaggtcc taaactggcg acctatggcc attttttctg 7800
gggtgatgca gtagaaggta agcgggtctt gttcccagcg gtcccatcca aggttcgcgg 7860
ctaggtctcg cgcggcagtc actagaggct catctccgcc gaacttcatg accagcatga 7920
agggcacgag ctgcttccca aaggccccca tccaagtata ggtctctaca tcgtaggtga 7980
caaagagacg ctcggtgcga ggatgcgagc cgatcgggaa gaactggatc tcccgccacc 8040
aattggagga gtggctattg atgtggtgaa agtagaagtc cctgcgacgg gccgaacact 8100
cgtgctggct tttgtaaaaa cgtgcgcagt actggcagcg gtgcacgggc tgtacatcct 8160
gcacgaggtt gacctgacga ccgcgcacaa ggaagcagag tgggaatttg agcccctcgc 8220
ctggcgggtt tggctggtgg tcttctactt cggctgcttg tccttgaccg tctggctgct 8280
cgaggggagt tacggtggat cggaccacca cgccgcgcga gcccaaagtc cagatgtccg 8340
cgcgcggcgg tcggagcttg atgacaacat cgcgcagatg ggagctgtcc atggtctgga 8400
gctcccgcgg cgtcaggtca ggcgggagct cctgcaggtt tacctcgcat agacgggtca 8460
gggcgcgggc tagatccagg tgatacctaa tttccagggg ctggttggtg gcggcgtcga 8520
tggcttgcaa gaggccgcat ccccgcggcg cgactacggt accgcgcggc gggcggtggg 8580
ccgcgggggt gtccttggat gatgcatcta aaagcggtga cgcgggcgag cccccggagg 8640
tagggggggc tccggacccg ccgggagagg gggcaggggc acgtcggcgc cgcgcgcggg 8700
caggagctgg tgctgcgcgc gtaggttgct ggcgaacgcg acgacgcggc ggttgatctc 8760
ctgaatctgg cgcctctgcg tgaagacgac gggcccggtg agcttgagcc tgaaagagag 8820
ttcgacagaa tcaatttcgg tgtcgttgac ggcggcctgg cgcaaaatct cctgcacgtc 8880
tcctgagttg tcttgatagg cgatctcggc catgaactgc tcgatctctt cctcctggag 8940
atctccgcgt ccggctcgct ccacggtggc ggcgaggtcg ttggaaatgc gggccatgag 9000
ctgcgagaag gcgttgaggc ctccctcgtt ccagacgcgg ctgtagacca cgcccccttc 9060
ggcatcgcgg gcgcgcatga ccacctgcgc gagattgagc tccacgtgcc gggcgaagac 9120
ggcgtagttt cgcaggcgct gaaagaggta gttgagggtg gtggcggtgt gttctgccac 9180
gaagaagtac ataacccagc gtcgcaacgt ggattcgttg atatccccca aggcctcaag 9240
gcgctccatg gcctcgtaga agtccacggc gaagttgaaa aactgggagt tgcgcgccga 9300
cacggttaac tcctcctcca gaagacggat gagctcggcg acagtgtcgc gcacctcgcg 9360
ctcaaaggct acaggggcct cttcttcttc ttcaatctcc tcttccataa gggcctcccc 9420
ttcttcttct tctggcggcg gtgggggagg ggggacacgg cggcgacgac ggcgcaccgg 9480
gaggcggtcg acaaagcgct cgatcatctc cccgcggcga cggcgcatgg tctcggtgac 9540
ggcgcggccg ttctcgcggg ggcgcagttg gaagacgccg cccgtcatgt cccggttatg 9600
ggttggcggg gggctgccat gcggcaggga tacggcgcta acgatgcatc tcaacaattg 9660
ttgtgtaggt actccgccgc cgagggacct gagcgagtcc gcatcgaccg gatcggaaaa 9720
cctctcgaga aaggcgtcta accagtcaca gtcgcaaggt aggctgagca ccgtggcggg 9780
cggcagcggg cggcggtcgg ggttgtttct ggcggaggtg ctgctgatga tgtaattaaa 9840
gtaggcggtc ttgagacggc ggatggtcga cagaagcacc atgtccttgg gtccggcctg 9900
ctgaatgcgc aggcggtcgg ccatgcccca ggcttcgttt tgacatcggc gcaggtcttt 9960
gtagtagtct tgcatgagcc tttctaccgg cacttcttct tctccttcct cttgtcctgc 10020
atctcttgca tctatcgctg cggcggcggc ggagtttggc cgtaggtggc gccctcttcc 10080
tcccatgcgt gtgaccccga agcccctcat cggctgaagc agggctaggt cggcgacaac 10140
gcgctcggct aatatggcct gctgcacctg cgtgagggta gactggaagt catccatgtc 10200
cacaaagcgg tggtatgcgc ccgtgttgat ggtgtaagtg cagttggcca taacggacca 10260
gttaacggtc tggtgacccg gctgcgagag ctcggtgtac ctgagacgcg agtaagccct 10320
cgagtcaaat acgtagtcgt tgcaagtccg caccaggtac tggtatccca ccaaaaagtg 10380
cggcggcggc tggcggtaga ggggccagcg tagggtggcc ggggctccgg gggcgagatc 10440
ttccaacata aggcgatgat atccgtagat gtacctggac atccaggtga tgccggcggc 10500
ggtggtggag gcgcgcggaa agtcgcggac gcggttccag atgttgcgca gcggcaaaaa 10560
gtgctccatg gtcgggacgc tctggccggt caggcgcgcg caatcgttga cgctctagac 10620
cgtgcaaaag gagagcctgt aagcgggcac tcttccgtgg tctggtggat aaattcgcaa 10680
gggtatcatg gcggacgacc ggggttcgag ccccgtatcc ggccgtccgc cgtgatccat 10740
gcggttaccg cccgcgtgtc gaacccaggt gtgcgacgtc agacaacggg ggagtgctcc 10800
ttttggcttc cttccaggcg cggcggctgc tgcgctagct tttttggcca ctggccgcgc 10860
gcagcgtaag cggttaggct ggaaagcgaa agcattaagt ggctcgctcc ctgtagccgg 10920
agggttattt tccaagggtt gagtcgcggg acccccggtt cgagtctcgg accggccgga 10980
ctgcggcgaa cgggggtttg cctccccgtc atgcaagacc ccgcttgcaa attcctccgg 11040
aaacagggac gagccccttt tttgcttttc ccagatgcat ccggtgctgc ggcagatgcg 11100
cccccctcct cagcagcggc aagagcaaga gcagcggcag acatgcaggg caccctcccc 11160
tcctcctacc gcgtcaggag gggcgacatc cgcggttgac gcggcagcag atggtgatta 11220
cgaacccccg cggcgccggg cccggcacta cctggacttg gaggagggcg agggcctggc 11280
gcggctagga gcgccctctc ctgagcggta cccaagggtg cagctgaagc gtgatacgcg 11340
tgaggcgtac gtgccgcggc agaacctgtt tcgcgaccgc gagggagagg agcccgagga 11400
gatgcgggat cgaaagttcc acgcagggcg cgagctgcgg catggcctga atcgcgagcg 11460
gttgctgcgc gaggaggact ttgagcccga cgcgcgaacc gggattagtc ccgcgcgcgc 11520
acacgtggcg gccgccgacc tggtaaccgc atacgagcag acggtgaacc aggagattaa 11580
ctttcaaaaa agctttaaca accacgtgcg tacgcttgtg gcgcgcgagg aggtggctat 11640
aggactgatg catctgtggg actttgtaag cgcgctggag caaaacccaa atagcaagcc 11700
gctcatggcg cagctgttcc ttatagtgca gcacagcagg gacaacgagg cattcaggga 11760
tgcgctgcta aacatagtag agcccgaggg ccgctggctg ctcgatttga taaacatcct 11820
gcagagcata gtggtgcagg agcgcagctt gagcctggct gacaaggtgg ccgccatcaa 11880
ctattccatg cttagcctgg gcaagtttta cgcccgcaag atataccata ccccttacgt 11940
tcccatagac aaggaggtaa agatcgaggg gttctacatg cgcatggcgc tgaaggtgct 12000
taccttgagc gacgacctgg gcgtttatcg caacgagcgc atccacaagg ccgtgagcgt 12060
gagccggcgg cgcgagctca gcgaccgcga gctgatgcac agcctgcaaa gggccctggc 12120
tggcacgggc agcggcgata gagaggccga gtcctacttt gacgcgggcg ctgacctgcg 12180
ctgggcccca agccgacgcg ccctggaggc agctggggcc ggacctgggc tggcggtggc 12240
acccgcgcgc gctggcaacg tcggcggcgt ggaggaatat gacgaggacg atgagtacga 12300
gccagaggac ggcgagtact aagcggtgat gtttctgatc agatgatgca agacgcaacg 12360
gacccggcgg tgcgggcggc gctgcagagc cagccgtccg gccttaactc cacggacgac 12420
tggcgccagg tcatggaccg catcatgtcg ctgactgcgc gcaatcctga cgcgttccgg 12480
cagcagccgc aggccaaccg gctctccgca attctggaag cggtggtccc ggcgcgcgca 12540
aaccccacgc acgagaaggt gctggcgatc gtaaacgcgc tggccgaaaa cagggccatc 12600
cggcccgacg aggccggcct ggtctacgac gcgctgcttc agcgcgtggc tcgttacaac 12660
agcggcaacg tgcagaccaa cctggaccgg ctggtggggg atgtgcgcga ggccgtggcg 12720
cagcgtgagc gcgcgcagca gcagggcaac ctgggctcca tggttgcact aaacgccttc 12780
ctgagtacac agcccgccaa cgtgccgcgg ggacaggagg actacaccaa ctttgtgagc 12840
gcactgcggc taatggtgac tgagacaccg caaagtgagg tgtaccagtc tgggccagac 12900
tattttttcc agaccagtag acaaggcctg cagaccgtaa acctgagcca ggctttcaaa 12960
aacttgcagg ggctgtgggg ggtgcgggct cccacaggcg accgcgcgac cgtgtctagc 13020
ttgctgacgc ccaactcgcg cctgttgctg ctgctaatag cgcccttcac ggacagtggc 13080
agcgtgtccc gggacacata cctaggtcac ttgctgacac tgtaccgcga ggccataggt 13140
caggcgcatg tggacgagca tactttccag gagattacaa gtgtcagccg cgcgctgggg 13200
caggaggaca cgggcagcct ggaggcaacc ctaaactacc tgctgaccaa ccggcggcag 13260
aagatcccct cgttgcacag tttaaacagc gaggaggagc gcattttgcg ctacgtgcag 13320
cagagcgtga gccttaacct gatgcgcgac ggggtaacgc ccagcgtggc gctggacatg 13380
accgcgcgca acatggaacc gggcatgtat gcctcaaacc ggccgtttat caaccgccta 13440
atggactact tgcatcgcgc ggccgccgtg aaccccgagt atttcaccaa tgccatcttg 13500
aacccgcact ggctaccgcc ccctggtttc tacaccgggg gattcgaggt gcccgagggt 13560
aacgatggat tcctctggga cgacatagac gacagcgtgt tttccccgca accgcagacc 13620
ctgctagagt tgcaacagcg cgagcaggca gaggcggcgc tgcgaaagga aagcttccgc 13680
aggccaagca gcttgtccga tctaggcgct gcggccccgc ggtcagatgc tagtagccca 13740
tttccaagct tgatagggtc tcttaccagc actcgcacca cccgcccgcg cctgctgggc 13800
gaggaggagt acctaaacaa ctcgctgctg cagccgcagc gcgaaaaaaa cctgcctccg 13860
gcatttccca acaacgggat agagagccta gtggacaaga tgagtagatg gaagacgtac 13920
gcgcaggagc acagggacgt gccaggcccg cgcccgccca cccgtcgtca aaggcacgac 13980
cgtcagcggg gtctggtgtg ggaggacgat gactcggcag acgacagcag cgtcctggat 14040
ttgggaggga gtggcaaccc gtttgcgcac cttcgcccca ggctggggag aatgttttaa 14100
aaaaaaaaaa gcatgatgca aaataaaaaa ctcaccaagg ccatggcacc gagcgttggt 14160
tttcttgtat tccccttagt atgcggcgcg cggcgatgta tgaggaaggt cctcctccct 14220
cctacgagag tgtggtgagc gcggcgccag tggcggcggc gctgggttct cccttcgatg 14280
ctcccctgga cccgccgttt gtgcctccgc ggtacctgcg gcctaccggg gggagaaaca 14340
gcatccgtta ctctgagttg gcacccctat tcgacaccac ccgtgtgtac ctggtggaca 14400
acaagtcaac ggatgtggca tccctgaact accagaacga ccacagcaac tttctgacca 14460
cggtcattca aaacaatgac tacagcccgg gggaggcaag cacacagacc atcaatcttg 14520
acgaccggtc gcactggggc ggcgacctga aaaccatcct gcataccaac atgccaaatg 14580
tgaacgagtt catgtttacc aataagttta aggcgcgggt gatggtgtcg cgcttgccta 14640
ctaaggacaa tcaggtggag ctgaaatacg agtgggtgga gttcacgctg cccgagggca 14700
actactccga gaccatgacc atagacctta tgaacaacgc gatcgtggag cactacttga 14760
aagtgggcag acagaacggg gttctggaaa gcgacatcgg ggtaaagttt gacacccgca 14820
acttcagact ggggtttgac cccgtcactg gtcttgtcat gcctggggta tatacaaacg 14880
aagccttcca tccagacatc attttgctgc caggatgcgg ggtggacttc acccacagcc 14940
gcctgagcaa cttgttgggc atccgcaagc ggcaaccctt ccaggagggc tttaggatca 15000
cctacgatga tctggagggt ggtaacattc ccgcactgtt ggatgtggac gcctaccagg 15060
cgagcttgaa agatgacacc gaacagggcg ggggtggcgc aggcggcagc aacagcagtg 15120
gcagcggcgc ggaagagaac tccaacgcgg cagccgcggc aatgcagccg gtggaggaca 15180
tgaacgatca tgccattcgc ggcgacacct ttgccacacg ggctgaggag aagcgcgctg 15240
aggccgaagc agcggccgaa gctgccgccc ccgctgcgca acccgaggtc gagaagcctc 15300
agaagaaacc ggtgatcaaa cccctgacag aggacagcaa gaaacgcagt tacaacctaa 15360
taagcaatga cagcaccttc acccagtacc gcagctggta ccttgcatac aactacggcg 15420
accctcagac cggaatccgc tcatggaccc tgctttgcac tcctgacgta acctgcggct 15480
cggagcaggt ctactggtcg ttgccagaca tgatgcaaga ccccgtgacc ttccgctcca 15540
cgcgccagat cagcaacttt ccggtggtgg gcgccgagct gttgcccgtg cactccaaga 15600
gcttctacaa cgaccaggcc gtctactccc aactcatccg ccagtttacc tctctgaccc 15660
acgtgttcaa tcgctttccc gagaaccaga ttttggcgcg cccgccagcc cccaccatca 15720
ccaccgtcag tgaaaacgtt cctgctctca cagatcacgg gacgctaccg ctgcgcaaca 15780
gcatcggagg agtccagcga gtgaccatta ctgacgccag acgccgcacc tgcccctacg 15840
tttacaaggc cctgggcata gtctcgccgc gcgtcctatc gagccgcact ttttgagcaa 15900
gcatgtccat ccttatatcg cccagcaata acacaggctg gggcctgcgc ttcccaagca 15960
agatgtttgg cggggccaag aagcgctccg accaacaccc agtgcgcgtg cgcgggcact 16020
accgcgcgcc ctggggcgcg cacaaacgcg gccgcactgg gcgcaccacc gtcgatgacg 16080
ccatcgacgc ggtggtggag gaggcgcgca actacacgcc cacgccgcca ccagtgtcca 16140
cagtggacgc ggccattcag accgtggtgc gcggagcccg gcgctatgct aaaatgaaga 16200
gacggcggag gcgcgtagca cgtcgccacc gccgccgacc cggcactgcc gcccaacgcg 16260
cggcggcggc cctgcttaac cgcgcacgtc gcaccggccg acgggcggcc atgcgggccg 16320
ctcgaaggct ggccgcgggt attgtcactg tgccccccag gtccaggcga cgagcggccg 16380
ccgcagcagc cgcggccatt agtgctatga ctcagggtcg caggggcaac gtgtattggg 16440
tgcgcgactc ggttagcggc ctgcgcgtgc ccgtgcgcac ccgccccccg cgcaactaga 16500
ttgcaagaaa aaactactta gactcgtact gttgtatgta tccagcggcg gcggcgcgca 16560
acgaagctat gtccaagcgc aaaatcaaag aagagatgct ccaggtcatc gcgccggaga 16620
tctatggccc cccgaagaag gaagagcagg attacaagcc ccgaaagcta aagcgggtca 16680
aaaagaaaaa gaaagatgat gatgatgaac ttgacgacga ggtggaactg ctgcacgcta 16740
ccgcgcccag gcgacgggta cagtggaaag gtcgacgcgt aaaacgtgtt ttgcgacccg 16800
gcaccaccgt agtctttacg cccggtgagc gctccacccg cacctacaag cgcgtgtatg 16860
atgaggtgta cggcgacgag gacctgcttg agcaggccaa cgagcgcctc ggggagtttg 16920
cctacggaaa gcggcataag gacatgctgg cgttgccgct ggacgagggc aacccaacac 16980
ctagcctaaa gcccgtaaca ctgcagcagg tgctgcccgc gcttgcaccg tccgaagaaa 17040
agcgcggcct aaagcgcgag tctggtgact tggcacccac cgtgcagctg atggtaccca 17100
agcgccagcg actggaagat gtcttggaaa aaatgaccgt ggaacctggg ctggagcccg 17160
aggtccgcgt gcggccaatc aagcaggtgg cgccgggact gggcgtgcag accgtggacg 17220
ttcagatacc cactaccagt agcaccagta ttgccaccgc cacagagggc atggagacac 17280
aaacgtcccc ggttgcctca gcggtggcgg atgccgcggt gcaggcggtc gctgcggccg 17340
cgtccaagac ctctacggag gtgcaaacgg acccgtggat gtttcgcgtt tcagcccccc 17400
ggcgcccgcg cggttcgagg aagtacggcg ccgccagcgc gctactgccc gaatatgccc 17460
tacatccttc cattgcgcct acccccggct atcgtggcta cacctaccgc cccagaagac 17520
gagcaactac ccgacgccga accaccactg gaacccgccg ccgccgtcgc cgtcgccagc 17580
ccgtgctggc cccgatttcc gtgcgcaggg tggctcgcga aggaggcagg accctggtgc 17640
tgccaacagc gcgctaccac cccagcatcg tttaaaagcc ggtctttgtg gttcttgcag 17700
atatggccct cacctgccgc ctccgtttcc cggtgccggg attccgagga agaatgcacc 17760
gtaggagggg catggccggc cacggcctga cgggcggcat gcgtcgtgcg caccaccggc 17820
ggcggcgcgc gtcgcaccgt cgcatgcgcg gcggtatcct gcccctcctt attccactga 17880
tcgccgcggc gattggcgcc gtgcccggaa ttgcatccgt ggccttgcag gcgcagagac 17940
actgattaaa aacaagttgc atgtggaaaa atcaaaataa aaagtctgga ctctcacgct 18000
cgcttggtcc tgtaactatt ttgtagaatg gaagacatca actttgcgtc tctggccccg 18060
cgacacggct cgcgcccgtt catgggaaac tggcaagata tcggcaccag caatatgagc 18120
ggtggcgcct tcagctgggg ctcgctgtgg agcggcatta aaaatttcgg ttccaccgtt 18180
aagaactatg gcagcaaggc ctggaacagc agcacaggcc agatgctgag ggataagttg 18240
aaagagcaaa atttccaaca aaaggtggta gatggcctgg cctctggcat tagcggggtg 18300
gtggacctgg ccaaccaggc agtgcaaaat aagattaaca gtaagcttga tccccgccct 18360
cccgtagagg agcctccacc ggccgtggag acagtgtctc cagaggggcg tggcgaaaag 18420
cgtccgcgcc ccgacaggga agaaactctg gtgacgcaaa tagacgagcc tccctcgtac 18480
gaggaggcac taaagcaagg cctgcccacc acccgtccca tcgcgcccat ggctaccgga 18540
gtgctgggcc agcacacacc cgtaacgctg gacctgcctc cccccgccga cacccagcag 18600
aaacctgtgc tgccaggccc gaccgccgtt gttgtaaccc gtcctagccg cgcgtccctg 18660
cgccgcgccg ccagcggtcc gcgatcgttg cggcccgtag ccagtggcaa ctggcaaagc 18720
acactgaaca gcatcgtggg tctgggggtg caatccctga agcgccgacg atgcttctga 18780
atagctaacg tgtcgtatgt gtgtcatgta tgcgtccatg tcgccgccag aggagctgct 18840
gagccgccgc gcgcccgctt tccaagatgg ctaccccttc gatgatgccg cagtggtctt 18900
acatgcacat ctcgggccag gacgcctcgg agtacctgag ccccgggctg gtgcagtttg 18960
cccgcgccac cgagacgtac ttcagcctga ataacaagtt tagaaacccc acggtggcgc 19020
ctacgcacga cgtgaccaca gaccggtccc agcgtttgac gctgcggttc atccctgtgg 19080
accgtgagga tactgcgtac tcgtacaagg cgcggttcac cctagctgtg ggtgataacc 19140
gtgtgctgga catggcttcc acgtactttg acatccgcgg cgtgctggac aggggcccta 19200
cttttaagcc ctactctggc actgcctaca acgccctggc tcccaagggt gccccaaatc 19260
cttgcgaatg ggatgaagct gctactgctc ttgaaataaa cctagaagaa gaggacgatg 19320
acaacgaaga cgaagtagac gagcaagctg agcagcaaaa aactcacgta tttgggcagg 19380
cgccttattc tggtataaat attacaaagg agggtattca aataggtgtc gaaggtcaaa 19440
cacctaaata tgccgataaa acatttcaac ctgaacctca aataggagaa tctcagtggt 19500
acgaaactga aattaatcat gcagctggga gagtccttaa aaagactacc ccaatgaaac 19560
catgttacgg ttcatatgca aaacccacaa atgaaaatgg agggcaaggc attcttgtaa 19620
agcaacaaaa tggaaagcta gaaagtcaag tggaaatgca atttttctca actactgagg 19680
cgaccgcagg caatggtgat aacttgactc ctaaagtggt attgtacagt gaagatgtag 19740
atatagaaac cccagacact catatttctt acatgcccac tattaaggaa ggtaactcac 19800
gagaactaat gggccaacaa tctatgccca acaggcctaa ttacattgct tttagggaca 19860
attttattgg tctaatgtat tacaacagca cgggtaatat gggtgttctg gcgggccaag 19920
catcgcagtt gaatgctgtt gtagatttgc aagacagaaa cacagagctt tcataccagc 19980
ttttgcttga ttccattggt gatagaacca ggtacttttc tatgtggaat caggctgttg 20040
acagctatga tccagatgtt agaattattg aaaatcatgg aactgaagat gaacttccaa 20100
attactgctt tccactggga ggtgtgatta atacagagac tcttaccaag gtaaaaccta 20160
aaacaggtca ggaaaatgga tgggaaaaag atgctacaga attttcagat aaaaatgaaa 20220
taagagttgg aaataatttt gccatggaaa tcaatctaaa tgccaacctg tggagaaatt 20280
tcctgtactc caacatagcg ctgtatttgc ccgacaagct aaagtacagt ccttccaacg 20340
taaaaatttc tgataaccca aacacctacg actacatgaa caagcgagtg gtggctcccg 20400
ggttagtgga ctgctacatt aaccttggag cacgctggtc ccttgactat atggacaacg 20460
tcaacccatt taaccaccac cgcaatgctg gcctgcgcta ccgctcaatg ttgctgggca 20520
atggtcgcta tgtgcccttc cacatccagg tgcctcagaa gttctttgcc attaaaaacc 20580
tccttctcct gccgggctca tacacctacg agtggaactt caggaaggat gttaacatgg 20640
ttctgcagag ctccctagga aatgacctaa gggttgacgg agccagcatt aagtttgata 20700
gcatttgcct ttacgccacc ttcttcccca tggcccacaa caccgcctcc acgcttgagg 20760
ccatgcttag aaacgacacc aacgaccagt cctttaacga ctatctctcc gccgccaaca 20820
tgctctaccc tatacccgcc aacgctacca acgtgcccat atccatcccc tcccgcaact 20880
gggcggcttt ccgcggctgg gccttcacgc gccttaagac taaggaaacc ccatcactgg 20940
gctcgggcta cgacccttat tacacctact ctggctctat accctaccta gatggaacct 21000
tttacctcaa ccacaccttt aagaaggtgg ccattacctt tgactcttct gtcagctggc 21060
ctggcaatga ccgcctgctt acccccaacg agtttgaaat taagcgctca gttgacgggg 21120
agggttacaa cgttgcccag tgtaacatga ccaaagactg gttcctggta caaatgctag 21180
ctaactacaa cattggctac cagggcttct atatcccaga gagctacaag gaccgcatgt 21240
actccttctt tagaaacttc cagcccatga gccgtcaggt ggtggatgat actaaataca 21300
aggactacca acaggtgggc atcctacacc aacacaacaa ctctggattt gttggctacc 21360
ttgcccccac catgcgcgaa ggacaggcct accctgctaa cttcccctat ccgcttatag 21420
gcaagaccgc agttgacagc attacccaga aaaagtttct ttgcgatcgc accctttggc 21480
gcatcccatt ctccagtaac tttatgtcca tgggcgcact cacagacctg ggccaaaacc 21540
ttctctacgc caactccgcc cacgcgctag acatgacttt tgaggtggat cccatggacg 21600
agcccaccct tctttatgtt ttgtttgaag tctttgacgt ggtccgtgtg caccggccgc 21660
accgcggcgt catcgaaacc gtgtacctgc gcacgccctt ctcggccggc aacgccacaa 21720
cataaagaag caagcaacat caacaacagc tgccgccatg ggctccagtg agcaggaact 21780
gaaagccatt gtcaaagatc ttggttgtgg gccatatttt ttgggcacct atgacaagcg 21840
ctttccaggc tttgtttctc cacacaagct cgcctgcgcc atagtcaata cggccggtcg 21900
cgagactggg ggcgtacact ggatggcctt tgcctggaac ccgcactcaa aaacatgcta 21960
cctctttgag ccctttggct tttctgacca gcgactcaag caggtttacc agtttgagta 22020
cgagtcactc ctgcgccgta gcgccattgc ttcttccccc gaccgctgta taacgctgga 22080
aaagtccacc caaagcgtac aggggcccaa ctcggccgcc tgtggactat tctgctgcat 22140
gtttctccac gcctttgcca actggcccca aactcccatg gatcacaacc ccaccatgaa 22200
ccttattacc ggggtaccca actccatgct caacagtccc caggtacagc ccaccctgcg 22260
tcgcaaccag gaacagctct acagcttcct ggagcgccac tcgccctact tccgcagcca 22320
cagtgcgcag attaggagcg ccacttcttt ttgtcacttg aaaaacatgt aaaaataatg 22380
tactagagac actttcaata aaggcaaatg cttttatttg tacactctcg ggtgattatt 22440
tacccccacc cttgccgtct gcgccgttta aaaatcaaag gggttctgcc gcgcatcgct 22500
atgcgccact ggcagggaca cgttgcgata ctggtgttta gtgctccact taaactcagg 22560
cacaaccatc cgcggcagct cggtgaagtt ttcactccac aggctgcgca ccatcaccaa 22620
cgcgtttagc aggtcgggcg ccgatatctt gaagtcgcag ttggggcctc cgccctgcgc 22680
gcgcgagttg cgatacacag ggttgcagca ctggaacact atcagcgccg ggtggtgcac 22740
gctggccagc acgctcttgt cggagatcag atccgcgtcc aggtcctccg cgttgctcag 22800
ggcgaacgga gtcaactttg gtagctgcct tcccaaaaag ggcgcgtgcc caggctttga 22860
gttgcactcg caccgtagtg gcatcaaaag gtgaccgtgc ccggtctggg cgttaggata 22920
cagcgcctgc ataaaagcct tgatctgctt aaaagccacc tgagcctttg cgccttcaga 22980
gaagaacatg ccgcaagact tgccggaaaa ctgattggcc ggacaggccg cgtcgtgcac 23040
gcagcacctt gcgtcggtgt tggagatctg caccacattt cggccccacc ggttcttcac 23100
gatcttggcc ttgctagact gctccttcag cgcgcgctgc ccgttttcgc tcgtcacatc 23160
catttcaatc acgtgctcct tatttatcat aatgcttccg tgtagacact taagctcgcc 23220
ttcgatctca gcgcagcggt gcagccacaa cgcgcagccc gtgggctcgt gatgcttgta 23280
ggtcacctct gcaaacgact gcaggtacgc ctgcaggaat cgccccatca tcgtcacaaa 23340
ggtcttgttg ctggtgaagg tcagctgcaa cccgcggtgc tcctcgttca gccaggtctt 23400
gcatacggcc gccagagctt ccacttggtc aggcagtagt ttgaagttcg cctttagatc 23460
gttatccacg tggtacttgt ccatcagcgc gcgcgcagcc tccatgccct tctcccacgc 23520
agacacgatc ggcacactca gcgggttcat caccgtaatt tcactttccg cttcgctggg 23580
ctcttcctct tcctcttgcg tccgcatacc acgcgccact gggtcgtctt cattcagccg 23640
ccgcactgtg cgcttacctc ctttgccatg cttgattagc accggtgggt tgctgaaacc 23700
caccatttgt agcgccacat cttctctttc ttcctcgctg tccacgatta cctctggtga 23760
tggcgggcgc tcgggcttgg gagaagggcg cttctttttc ttcttgggcg caatggccaa 23820
atccgccgcc gaggtcgatg gccgcgggct gggtgtgcgc ggcaccagcg cgtcttgtga 23880
tgagtcttcc tcgtcctcgg actcgatacg ccgcctcatc cgcttttttg ggggcgcccg 23940
gggaggcggc ggcgacgggg acggggacga cacgtcctcc atggttgggg gacgtcgcgc 24000
cgcaccgcgt ccgcgctcgg gggtggtttc gcgctgctcc tcttcccgac tggccatttc 24060
cttctcctat aggcagaaaa agatcatgga gtcagtcgag aagaaggaca gcctaaccgc 24120
cccctctgag ttcgccacca ccgcctccac cgatgccgcc aacgcgccta ccaccttccc 24180
cgtcgaggca cccccgcttg aggaggagga agtgattatc gagcaggacc caggttttgt 24240
aagcgaagac gacgaggacc gctcagtacc aacagaggat aaaaagcaag accaggacaa 24300
cgcagaggca aacgaggaac aagtcgggcg gggggacgaa aggcatggcg actacctaga 24360
tgtgggagac gacgtgctgt tgaagcatct gcagcgccag tgcgccatta tctgcgacgc 24420
gttgcaagag cgcagcgatg tgcccctcgc catagcggat gtcagccttg cctacgaacg 24480
ccacctattc tcaccgcgcg taccccccaa acgccaagaa aacggcacat gcgagcccaa 24540
cccgcgcctc aacttctacc ccgtatttgc cgtgccagag gtgcttgcca cctatcacat 24600
ctttttccaa aactgcaaga tacccctatc ctgccgtgcc aaccgcagcc gagcggacaa 24660
gcagctggcc ttgcggcagg gcgctgtcat acctgatatc gcctcgctca acgaagtgcc 24720
aaaaatcttt gagggtcttg gacgcgacga gaagcgcgcg gcaaacgctc tgcaacagga 24780
aaacagcgaa aatgaaagtc actctggagt gttggtggaa ctcgagggtg acaacgcgcg 24840
cctagccgta ctaaaacgca gcatcgaggt cacccacttt gcctacccgg cacttaacct 24900
accccccaag gtcatgagca cagtcatgag tgagctgatc gtgcgccgtg cgcagcccct 24960
ggagagggat gcaaatttgc aagaacaaac agaggagggc ctacccgcag ttggcgacga 25020
gcagctagcg cgctggcttc aaacgcgcga gcctgccgac ttggaggagc gacgcaaact 25080
aatgatggcc gcagtgctcg ttaccgtgga gcttgagtgc atgcagcggt tctttgctga 25140
cccggagatg cagcgcaagc tagaggaaac attgcactac acctttcgac agggctacgt 25200
acgccaggcc tgcaagatct ccaacgtgga gctctgcaac ctggtctcct accttggaat 25260
tttgcacgaa aaccgccttg ggcaaaacgt gcttcattcc acgctcaagg gcgaggcgcg 25320
ccgcgactac gtccgcgact gcgtttactt atttctatgc tacacctggc agacggccat 25380
gggcgtttgg cagcagtgct tggaggagtg caacctcaag gagctgcaga aactgctaaa 25440
gcaaaacttg aaggacctat ggacggcctt caacgagcgc tccgtggccg cgcacctggc 25500
ggacatcatt ttccccgaac gcctgcttaa aaccctgcaa cagggtctgc cagacttcac 25560
cagtcaaagc atgttgcaga actttaggaa ctttatccta gagcgctcag gaatcttgcc 25620
cgccacctgc tgtgcacttc ctagcgactt tgtgcccatt aagtaccgcg aatgccctcc 25680
gccgctttgg ggccactgct accttctgca gctagccaac taccttgcct accactctga 25740
cataatggaa gacgtgagcg gtgacggtct actggagtgt cactgtcgct gcaacctatg 25800
caccccgcac cgctccctgg tttgcaattc gcagctgctt aacgaaagtc aaattatcgg 25860
tacctttgag ctgcagggtc cctcgcctga cgaaaagtcc gcggctccgg ggttgaaact 25920
cactccgggg ctgtggacgt cggcttacct tcgcaaattt gtacctgagg actaccacgc 25980
ccacgagatt aggttctacg aagaccaatc ccgcccgcca aatgcggagc ttaccgcctg 26040
cgtcattacc cagggccaca ttcttggcca attgcaagcc atcaacaaag cccgccaaga 26100
gtttctgcta cgaaagggac ggggggttta cttggacccc cagtccggcg aggagctcaa 26160
cccaatcccc ccgccgccgc agccctatca gcagcagccg cgggcccttg cttcccagga 26220
tggcacccaa aaagaagctg cagctgccgc cgccacccac ggacgaggag gaatactggg 26280
acagtcaggc agaggaggtt ttggacgagg aggaggagga catgatggaa gactgggaga 26340
gcctagacga ggaagcttcc gaggtcgaag aggtgtcaga cgaaacaccg tcaccctcgg 26400
tcgcattccc ctcgccggcg ccccagaaat cggcaaccgg ttccagcatg gctacaacct 26460
ccgctcctca ggcgccgccg gcactgcccg ttcgccgacc caaccgtaga tgggacacca 26520
ctggaaccag ggccggtaag tccaagcagc cgccgccgtt agcccaagag caacaacagc 26580
gccaaggcta ccgctcatgg cgcgggcaca agaacgccat agttgcttgc ttgcaagact 26640
gtgggggcaa catctccttc gcccgccgct ttcttctcta ccatcacggc gtggccttcc 26700
cccgtaacat cctgcattac taccgtcatc tctacagccc atactgcacc ggcggcagcg 26760
gcagcggcag caacagcagc ggccacacag aagcaaaggc gaccggatag caagactctg 26820
acaaagccca agaaatccac agcggcggca gcagcaggag gaggagcgct gcgtctggcg 26880
cccaacgaac ccgtatcgac ccgcgagctt agaaacagga tttttcccac tctgtatgct 26940
atatttcaac agagcagggg ccaagaacaa gagctgaaaa taaaaaacag gtctctgcga 27000
tccctcaccc gcagctgcct gtatcacaaa agcgaagatc agcttcggcg cacgctggaa 27060
gacgcggagg ctctcttcag taaatactgc gcgctgactc ttaaggacta gtttcgcgcc 27120
ctttctcaaa tttaagcgcg aaaactacgt catctccagc ggccacaccc ggcgccagca 27180
cctgtcgtca gcgccatttc aactttgtat acaaaagttg tgatgagcaa ggaaattccc 27240
acgccctaca tgtggagtta ccagccacaa atgggacttg cggctggagc tgcccaagac 27300
tactcaaccc gaataaacta catgagcgcg ggaccccaca tgatatcccg ggtcaacgga 27360
atacgcgccc accgaaaccg aattctcctg gaacaggcgg ctattaccac cacacctcgt 27420
aataacctta atccccgtag ttggcccgct gccctggtgt accaggaaag tcccgctccc 27480
accactgtgg tacttcccag agacgcccag gccgaagttc agatgactaa ctcaggggcg 27540
cagcttgcgg gcggctttcg tcacagggtg cggtcgcccg ggcagggtat aactcacctg 27600
acaatcagag ggcgaggtat tcagctcaac gacgagtcgg tgagctcctc gcttggtctc 27660
cgtccggacg ggacatttca gatcggcggc gccggccgct cttcattcac gcctcgtcag 27720
gcaatcctaa ctctgcagac ctcgtcctct gagccgcgct ctggaggcat tggaactctg 27780
caatttattg aggagtttgt gccatcggtc tactttaacc ccttctcggg acctcccggc 27840
cactatccgg atcaatttat tcctaacttt gacgcggtaa aggactcggc ggacggctac 27900
gactgaatgt taagtggaga ggcagagcaa ctgcgcctga aacacctggt ccactgtcgc 27960
cgccacaagt gctttgcccg cgactccggt gagttttgct actttgaatt gcccgaggat 28020
catatcgagg gcccggcgca cggcgtccgg cttaccgccc agggagagct tgcccgtagc 28080
ctgattcggg agtttaccca gcgccccctg ctagttgagc gggacagggg accctgtgtt 28140
ctcactgtga tttgcaactg tcctaaccct ggattacatc aagatctttg ttgccatctc 28200
tgtgctgagt ataataaata cagaaattaa aatatactgg ggctcctatc gccatcctgt 28260
aaacgccacc gtcttcaccc gcccaagcaa accaaggcga accttacctg gtacttttaa 28320
catctctccc tctgtgattt acaacagttt caacccagac ggagtgagtc tacgagagaa 28380
cctctccgag ctcagctact ccatcagaaa aaacaccacc ctccttacct gccgggaacg 28440
tacgagtgcg tcaccggccg ctgcaccaca cctaccgcct gaccgtaaac cagacttttt 28500
ccggacagac ctcaataact ctgtttacca gaacaggagg tgagcttaga aaacccttag 28560
ggtattaggc caaaggcgca gctactgtgg ggtttatgaa caattcaagc aactctacgg 28620
gctattctaa ttcaggtttc tctagaatcg gggttggggt tattctctgt cttgtgattc 28680
tctttattct tatactaacg cttctctgcc taaggctcgc cgcctgctgt gtgcacattt 28740
gcatttattg tcagcttttt aaacgctggg gtcgccaccc aagatgatta ggtacataat 28800
cctaggttta ctcacccttg cgtcagccca cggtaccacc caaaaggtgg attttaagga 28860
gccagcctgt aatgttacat tcgcagctga agctaatgag tgcaccactc ttataaaatg 28920
caccacagaa catgaaaagc tgcttattcg ccacaaaaac aaaattggca agtatgctgt 28980
ttatgctatt tggcagccag gtgacactac agagtataat gttacagttt tccagggtaa 29040
aagtcataaa acttttatgt atacttttcc attttatgaa atgtgcgaca ttaccatgta 29100
catgagcaaa cagtataagt tgtggccccc acaaaattgt gtggaaaaca ctggcacttt 29160
ctgctgcact gctatgctaa ttacagtgct cgctttggtc tgtaccctac tctatattaa 29220
atacaaaagc agacgcagct ttattgagga aaagaaaatg ccttaattta ctaagttaca 29280
aagctaatgt caccactaac tgctttactc gctgcttgca aaacaaattc aaaaagttag 29340
cattataatt agaataggat ttaaaccccc cggtcatttc ctgctcaata ccattcccct 29400
gaacaattga ctctatgtgg gatatgctcc agcgctacaa ccttgaagtc aggcttcctg 29460
gatgtcagca tctgactttg gccagcacct gtcccgcgga tttgttccag tccaactaca 29520
gcgacccacc ctaacagaga tgaccaacac aaccaacgcg gccgccgcta ccggacttac 29580
atctaccaca aatacacccc aagtttctgc ctttgtcaat aactgggata acttgggcat 29640
gtggtggttc tccatagcgc ttatgtttgt atgccttatt attatgtggc tcatctgctg 29700
cctaaagcgc aaacgcgccc gaccacccat ctatagtccc atcattgtgc tacacccaaa 29760
caatgatgga atccatagat tggacggact gaaacacatg ttcttttctc ttacagtatg 29820
attaaatgag acatgattcc tcgagttttt atattactga cccttgttgc gcttttttgt 29880
gcgtgctcca cattggctgc ggtttctcac atcgaagtag actgcattcc agccttcaca 29940
gtctatttgc tttacggatt tgtcaccctc acgctcatct gcagcctcat cactgtggtc 30000
atcgccttta tccagtgcat tgactgggtc tgtgtgcgct ttgcatatct cagacaccat 30060
ccccagtaca gggacaggac tatagctgag cttcttagaa ttctttaatt atgaaattta 30120
ctgtgacttt tctgctgatt atttgcaccc tatctgcgtt ttgttccccg acctccaagc 30180
ctcaaagaca tatatcatgc agattcactc gtatatggaa tattccaagt tgctacaatg 30240
aaaaaagcga tctttccgaa gcctggttat atgcaatcat ctctgttatg gtgttctgca 30300
gtaccatctt agccctagct atatatccct accttgacat tggctggaac gcaatagatg 30360
ccatgaacca cccaactttc cccgcgcccg ctatgcttcc actgcaacaa gttgttgccg 30420
gcggctttgt cccagccaat cagcctcgcc caccttctcc cacccccact gaaatcagct 30480
actttaatct aacaggagga gatgactgac accctagatc tagaaatgga cggaattatt 30540
acagagcagc gcctgctaga aagacgcagg gcagcggccg agcaacagcg catgaatcaa 30600
gagctccaag acatggttaa cttgcaccag tgcaaaaggg gtatcttttg tctggtaaag 30660
caggccaaag tcacctacga cagtaatacc accggacacc gccttagcta caagttgcca 30720
accaagcgtc agaaattggt ggtcatggtg ggagaaaagc ccattaccat aactcagcac 30780
tcggtagaaa ccgaaggctg cattcactca ccttgtcaag gacctgagga tctctgcacc 30840
cttattaaga ccctgtgcgg tctcaaagat cttattccct ttaactaata aaaaaaaata 30900
ataaagcatc acttacttaa aatcagttag caaatttctg tccagtttat tcagcagcac 30960
ctccttgccc tcctcccagc tctggtattg cagcttcctc ctggctgcaa actttctcca 31020
caatctaaat ggaatgtcag tttcctcctg ttcctgtcca tccgcaccca ctatcttcat 31080
gttgttgcag atgaagcgcg caagaccgtc tgaagatacc ttcaaccccg tgtatccata 31140
tgacacggaa accggtcctc caactgtgcc ttttcttact cctccctttg tatcccccaa 31200
tgggtttcaa gagagtcccc ctggggtact ctctttgcgc ctatccgaac ctctagttac 31260
ctccaatggc atgcttgcgc tcaaaatggg caacggcctc tctctggacg aggccggcaa 31320
ccttacctcc caaaatgtaa ccactgtgag cccacctctc aaaaaaacca agtcaaacat 31380
aaacctggaa atatctgcac ccctcacagt tacctcagaa gccctaactg tggctgccgc 31440
cgcacctcta atggtcgcgg gcaacacact caccatgcaa tcacaggccc cgctaaccgt 31500
gcacgactcc aaacttagca ttgccaccca aggacccctc acagtgtcag aaggaaagct 31560
agccctgcaa acatcaggcc ccctcaccac caccgatagc agtaccctta ctatcactgc 31620
ctcaccccct ctaactactg ccactggtag cttgggcatt gacttgaaag agcccattta 31680
tacacaaaat ggaaaactag gactaaagta cggggctcct ttgcatgtaa cagacgacct 31740
aaacactttg accgtagcaa ctggtccagg tgtgactatt aataatactt ccttgcaaac 31800
taaagttact ggagccttgg gttttgattc acaaggcaat atgcaactta atgtagcagg 31860
aggactaagg attgattctc aaaacagacg ccttatactt gatgttagtt atccgtttga 31920
tgctcaaaac caactaaatc taagactagg acagggccct ctttttataa actcagccca 31980
caacttggat attaactaca acaaaggcct ttacttgttt acagcttcaa acaattccaa 32040
aaagcttgag gttaacctaa gcactgccaa ggggttgatg tttgacgcta cagccatagc 32100
cattaatgca ggagatgggc ttgaatttgg ttcacctaat gcaccaaaca caaatcccct 32160
caaaacaaaa attggccatg gcctagaatt tgattcaaac aaggctatgg ttcctaaact 32220
aggaactggc cttagttttg acagcacagg tgccattaca gtaggaaaca aaaataatga 32280
taagctaact ttgtggacca caccagctcc atctcctaac tgtagactaa atgcagagaa 32340
agatgctaaa ctcactttgg tcttaacaaa atgtggcagt caaatacttg ctacagtttc 32400
agttttggct gttaaaggca gtttggctcc aatatctgga acagttcaaa gtgctcatct 32460
tattataaga tttgacgaaa atggagtgct actaaacaat tccttcctgg acccagaata 32520
ttggaacttt agaaatggag atcttactga aggcacagcc tatacaaacg ctgttggatt 32580
tatgcctaac ctatcagctt atccaaaatc tcacggtaaa actgccaaaa gtaacattgt 32640
cagtcaagtt tacttaaacg gagacaaaac taaacctgta acactaacca ttacactaaa 32700
cggtacacag gaaacaggag acacaactcc aagtgcatac tctatgtcat tttcatggga 32760
ctggtctggc cacaactaca ttaatgaaat atttgccaca tcctcttaca ctttttcata 32820
cattgcccaa gaataaagaa tcgtttgtgt tatgtttcaa cgtgtttatt tttcaattgc 32880
agaaaatttc aagtcatttt tcattcagta gtatagcccc accaccacat agcttataca 32940
gatcaccgta cctcaacttt gtataataaa gttgaatcaa actcacagaa ccctagtatt 33000
caacctgcca cctccctccc aacacacaga gtacacagtc ctttctcccc ggctggcctt 33060
aaaaagcatc atatcatggg taacagacat attcttaggt gttatattcc acacggtttc 33120
ctgtcgagcc aaacgctcat cagtgatatt aataaactcc ccgggcagct cacttaagtt 33180
catgtcgctg tccagctgct gagccacagg ctgctgtcca acttgcggtt gcttaacggg 33240
cggcgaagga gaagtccacg cctacatggg ggtagagtca taatcgtgca tcaggatagg 33300
gcggtggtgc tgcagcagcg cgcgaataaa ctgctgccgc cgccgctccg tcctgcagga 33360
atacaacatg gcagtggtct cctcagcgat gattcgcacc gcccgcagca taaggcgcct 33420
tgtcctccgg gcacagcagc gcaccctgat ctcacttaaa tcagcacagt aactgcagca 33480
cagcaccaca atattgttca aaatcccaca gtgcaaggcg ctgtatccaa agctcatggc 33540
ggggaccaca gaacccacgt ggccatcata ccacaagcgc aggtagatta agtggcgacc 33600
cctcataaac acgctggaca taaacattac ctcttttggc atgttgtaat tcaccacctc 33660
ccggtaccat ataaacctct gattaaacat ggcgccatcc accaccatcc taaaccagct 33720
ggccaaaacc tgcccgccgg ctatacactg cagggaaccg ggactggaac aatgacagtg 33780
gagagcccag gactcgtaac catggatcat catgctcgtc atgatatcaa tgttggcaca 33840
acacaggcac acgtgcatac acttcctcag gattacaagc tcctcccgcg ttagaaccat 33900
atcccaggga acaacccatt cctgaatcag cgtaaatccc acactgcagg gaagacctcg 33960
cacgtaactc acgttgtgca ttgtcaaagt gttacattcg ggcagcagcg gatgatcctc 34020
cagtatggta gcgcgggttt ctgtctcaaa aggaggtaga cgatccctac tgtacggagt 34080
gcgccgagac aaccgagatc gtgttggtcg tagtgtcatg ccaaatggaa cgccggacgt 34140
agtcatattt cctgaagcaa aaccaggtgc gggcgtgaca aacagatctg cgtctccggt 34200
ctcgccgctt agatcgctct gtgtagtagt tgtagtatat ccactctctc aaagcatcca 34260
ggcgccccct ggcttcgggt tctatgtaaa ctccttcatg cgccgctgcc ctgataacat 34320
ccaccaccgc agaataagcc acacccagcc aacctacaca ttcgttctgc gagtcacaca 34380
cgggaggagc gggaagagct ggaagaacca tgtttttttt tttattccaa aagattatcc 34440
aaaacctcaa aatgaagatc tattaagtga acgcgctccc ctccggtggc gtggtcaaac 34500
tctacagcca aagaacagat aatggcattt gtaagatgtt gcacaatggc ttccaaaagg 34560
caaacggccc tcacgtccaa gtggacgtaa aggctaaacc cttcagggtg aatctcctct 34620
ataaacattc cagcaccttc aaccatgccc aaataattct catctcgcca ccttctcaat 34680
atatctctaa gcaaatcccg aatattaagt ccggccattg taaaaatctg ctccagagcg 34740
ccctccacct tcagcctcaa gcagcgaatc atgattgcaa aaattcaggt tcctcacaga 34800
cctgtataag attcaaaagc ggaacattaa caaaaatacc gcgatcccgt aggtcccttc 34860
gcagggccag ctgaacataa tcgtgcaggt ctgcacggac cagcgcggcc acttccccgc 34920
caggaaccat gacaaaagaa cccacactga ttatgacacg catactcgga gctatgctaa 34980
ccagcgtagc cccgatgtaa gcttgttgca tgggcggcga tataaaatgc aaggtgctgc 35040
tcaaaaaatc aggcaaagcc tcgcgcaaaa aagaaagcac atcgtagtca tgctcatgca 35100
gataaaggca ggtaagctcc ggaaccacca cagaaaaaga caccattttt ctctcaaaca 35160
ttgggccagg attctcctcg acgtcaccag cctgcttcag caggctgaag ttagtagctc 35220
cgcttccctt atagagctcg ttcatgccct cggtgatgcc ggcggcggtc aggaactcca 35280
gcagcaccat gtggtcccgc ttctcgttgg ggtccttgaa cagggcgctc tggtagctca 35340
ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt 35400
ggtcggccag ctgcacgccg ccgtcctcga tgttgtgccg gatcttgaag ttggccttga 35460
tgccgttctt ctgcttgtcg gcggtgatgt acacgttgtg gctgttgtag ttgtactcca 35520
gcttgtggcc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatccggt 35580
tcaccagggt gtcgccctcg aacttcacct cggcccgggt cttgtagttg ccgtcgtcct 35640
tgaagaagat ggtccgctcc tgcacgtagc cctcgggcat ggcgctcttg aagaagtcgt 35700
gctgcttcat gtggtcgggg taccgggcga agcactgcac gccgtagccc agggtggtca 35760
ccagggtggg ccagggcacg ggcagcttgc cggtggtgca cagcagcttc agggtcagct 35820
tgccgtaggt ggcgtcgccc tcgccctcgc cgctcacgct gaacttgtgg ccgttcacgt 35880
cgccgtccag ctccaccagg atgggcacca cgccggtgaa cagctcttcg cctttgctca 35940
ccatgtctgc gggtttctgc ataaacacaa aataaaataa caaaaaaaca tttaaacatt 36000
agaagcctgt cttacaacag gaaaaacaac ccttataagc ataagacgga ctacggccat 36060
gccggcgtga ccgtaaaaaa actggtcacc gtgattaaaa agcaccaccg acagctcctc 36120
ggtcatgtcc ggagtcataa tgtaagactc ggtaaacaca tcaggttgat tcacatcggt 36180
cagtgctaaa aagcgaccga aatagcccgg gggaatacat acccgcaggc gtagagacaa 36240
cattacagcc cccataggag gtataacaaa attaatagga gagaaaaaca cataaacacc 36300
tgaaaaaccc tcctgcctag gcaaaatagc accctcccgc tccagaacaa catacagcgc 36360
ttccacagcg gcagccataa cagtcagcct taccagtaaa aaagaaaacc tattaaaaaa 36420
acaccactcg acacggcacc agctcaatca gtcacagtgt aaaaaagggc caagtgcaga 36480
gcgagtatat ataggactaa aaaatgacgt aacggttaaa gtccacaaaa aacacccaga 36540
aaaccgcacg cgaacctacg cccagaaacg aaagccaaaa aacccacaac ttcctcaaat 36600
cgtcacttcc gttttcccac gttacgtcac ttcccatttt aagaaaacta caattcccaa 36660
cacatacaag ttactccgcc ctaaaaccta cgtcacccgc cccgttccca cgccccgcgc 36720
cacgtcacaa actccacccc ctcattatca tattggcttc aatccaaaat aaggtatatt 36780
attgatgatg 36790
<210> 11
<211> 36781
<212> DNA
<213> 人工序列
<220>
<223> 合成腺病毒基因组mCherry-P2A-E4-ORF2
<400> 11
catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60
ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120
gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180
gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240
taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300
agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360
gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420
cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480
tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540
tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600
aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660
tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720
cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780
gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840
cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900
ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960
cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020
caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080
ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140
tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200
gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260
ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320
cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380
ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440
gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500
cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560
ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620
gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680
cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740
ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800
tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860
gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920
caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980
gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040
agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100
aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160
cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220
gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280
gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340
gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400
gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460
tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520
ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580
tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640
agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700
tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760
gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820
acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880
gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940
aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000
ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060
gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120
tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180
acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240
aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300
tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360
gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420
tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480
ttggctctag cgatgaagat acagattgag gtactgaaat gtgtgggcgt ggcttaaggg 3540
tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg 3600
ccgccgccca acttttctat acaaagttgt aatgagcacc aactcgtttg atggaagcat 3660
tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat 3720
gggctccagc attgatggtc gccccgtcct gcccgcaaac tctactacct tgacctacga 3780
gaccgtgtct ggaacgccgt tggagactgc agcctccgcc gccgcttcag ccgctgcagc 3840
caccgcccgc gggattgtga ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc 3900
ttcccgttca tccgcccgcg atgacaagtt gacggctctt ttggcacaat tggattcttt 3960
gacccgggaa cttaatgtcg tttctcagca gctgttggat ctgcgccagc aggtttctgc 4020
cctgaaggct tcctcccctc ccaatgcggt ttaaaacata aataaaaaac cagactctgt 4080
ttggatttgg atcaagcata agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg 4140
taggcccggg accagcggtc tcggtcgttg agggtcctgt gtattttttc caggacgtgg 4200
taaaggtgac tctggatgtt cagatacatg ggcataagcc cgtctctggg gtggaggtag 4260
caccactgca gagcttcatg ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag 4320
cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc tgattgccag gggcaggccc 4380
ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt gcatacgtgg ggatatgaga 4440
tgcatcttgg actgtatttt taggttggct atgttcccag ccatatccct ccggggattc 4500
atgttgtgca gaaccaccag cacagtgtat ccggtgcact tgggaaattt gtcatgtagc 4560
ttagaaggaa atgcgtggaa gaacttggag acgcccttgt gacctccaag attttccatg 4620
cattcgtcca taatgatggc aatgggccca cgggcggcgg cctgggcgaa gatatttctg 4680
ggatcactaa cgtcatagtt gtgttccagg atgagatcgt cataggccat ttttacaaag 4740
cgcgggcgga gggtgccaga ctgcggtata atggttccat ccggcccagg ggcgtagtta 4800
ccctcacaga tttgcatttc ccacgctttg agttcagatg gggggatcat gtctacctgc 4860
ggggcgatga agaaaacggt ttccggggta ggggagatca gctgggaaga aagcaggttc 4920
ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa tcacacctat taccgggtgc 4980
aactggtagt taagagagct gcagctgccg tcatccctga gcaggggggc cacttcgtta 5040
agcatgtccc tgactcgcat gttttccctg accaaatccg ccagaaggcg ctcgccgccc 5100
agcgatagca gttcttgcaa ggaagcaaag tttttcaacg gtttgagacc gtccgccgta 5160
ggcatgcttt tgagcgtttg accaagcagt tccaggcggt cccacagctc ggtcacctgc 5220
tctacggcat ctcgatccag catatctcct cgtttcgcgg gttggggcgg ctttcgctgt 5280
acggcagtag tcggtgctcg tccagacggg ccagggtcat gtctttccac gggcgcaggg 5340
tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc tccgggctgc gcgctggcca 5400
gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt 5460
cggccaggta gcatttgacc atggtgtcat agtccagccc ctccgcggcg tggcccttgg 5520
cgcgcagctt gcccttggag gaggcgccgc acgaggggca gtgcagactt ttgagggcgt 5580
agagcttggg cgcgagaaat accgattccg gggagtaggc atccgcgccg caggccccgc 5640
agacggtctc gcattccacg agccaggtga gctctggccg ttcggggtca aaaaccaggt 5700
ttcccccatg ctttttgatg cgtttcttac ctctggtttc catgagccgg tgtccacgct 5760
cggtgacgaa aaggctgtcc gtgtccccgt atacagactt gagaggcctg tcctcgagcg 5820
gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacaaag gctcgcgtcc 5880
aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact agggggtcca 5940
ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag gtgattggtt 6000
tgtaggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag ggggtggggg 6060
cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt tggggtgagt 6120
actccctctg aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg 6180
aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgca tccatctggt 6240
cagaaaagac aatctttttg ttgtcaagct tggtggcaaa cgacccgtag agggcgttgg 6300
acagcaactt ggcgatggag cgcagggttt ggtttttgtc gcgatcggcg cgctccttgg 6360
ccgcgatgtt tagctgcacg tattcgcgcg caacgcaccg ccattcggga aagacggtgg 6420
tgcgctcgtc gggcaccagg tgcacgcgcc aaccgcggtt gtgcagggtg acaaggtcaa 6480
cgctggtggc tacctctccg cgtaggcgct cgttggtcca gcagaggcgg ccgcccttgc 6540
gcgagcagaa tggcggtagg gggtctagct gcgtctcgtc cggggggtct gcgtccacgg 6600
taaagacccc gggcagcagg cgcgcgtcga agtagtctat cttgcatcct tgcaagtcta 6660
gcgcctgctg ccatgcgcgg gcggcaagcg cgcgctcgta tgggttgagt gggggacccc 6720
atggcatggg gtgggtgagc gcggaggcgt acatgccgca aatgtcgtaa acgtagaggg 6780
gctctctgag tattccaaga tatgtagggt agcatcttcc accgcggatg ctggcgcgca 6840
cgtaatcgta tagttcgtgc gagggagcga ggaggtcggg accgaggttg ctacgggcgg 6900
gctgctctgc tcggaagact atctgcctga agatggcatg tgagttggat gatatggttg 6960
gacgctggaa gacgttgaag ctggcgtctg tgagacctac cgcgtcacgc acgaaggagg 7020
cgtaggagtc gcgcagcttg ttgaccagct cggcggtgac ctgcacgtct agggcgcagt 7080
agtccagggt ttccttgatg atgtcatact tatcctgtcc cttttttttc cacagctcgc 7140
ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct 7200
ccgaacggta agagcctagc atgtagaact ggttgacggc ctggtaggcg cagcatccct 7260
tttctacggg tagcgcgtat gcctgcgcgg ccttccggag cgaggtgtgg gtgagcgcaa 7320
aggtgtccct gaccatgact ttgaggtact ggtatttgaa gtcagtgtcg tcgcatccgc 7380
cctgctccca gagcaaaaag tccgtgcgct ttttggaacg cggatttggc agggcgaagg 7440
tgacatcgtt gaagagtatc tttcccgcgc gaggcataaa gttgcgtgtg atgcggaagg 7500
gtcccggcac ctcggaacgg ttgttaatta cctgggcggc gagcacgatc tcgtcaaagc 7560
cgttgatgtt gtggcccaca atgtaaagtt ccaagaagcg cgggatgccc ttgatggaag 7620
gcaatttttt aagttcctcg taggtgagct cttcagggga gctgagcccg tgctctgaaa 7680
gggcccagtc tgcaagatga gggttggaag cgacgaatga gctccacagg tcacgggcca 7740
ttagcatttg caggtggtcg cgaaaggtcc taaactggcg acctatggcc attttttctg 7800
gggtgatgca gtagaaggta agcgggtctt gttcccagcg gtcccatcca aggttcgcgg 7860
ctaggtctcg cgcggcagtc actagaggct catctccgcc gaacttcatg accagcatga 7920
agggcacgag ctgcttccca aaggccccca tccaagtata ggtctctaca tcgtaggtga 7980
caaagagacg ctcggtgcga ggatgcgagc cgatcgggaa gaactggatc tcccgccacc 8040
aattggagga gtggctattg atgtggtgaa agtagaagtc cctgcgacgg gccgaacact 8100
cgtgctggct tttgtaaaaa cgtgcgcagt actggcagcg gtgcacgggc tgtacatcct 8160
gcacgaggtt gacctgacga ccgcgcacaa ggaagcagag tgggaatttg agcccctcgc 8220
ctggcgggtt tggctggtgg tcttctactt cggctgcttg tccttgaccg tctggctgct 8280
cgaggggagt tacggtggat cggaccacca cgccgcgcga gcccaaagtc cagatgtccg 8340
cgcgcggcgg tcggagcttg atgacaacat cgcgcagatg ggagctgtcc atggtctgga 8400
gctcccgcgg cgtcaggtca ggcgggagct cctgcaggtt tacctcgcat agacgggtca 8460
gggcgcgggc tagatccagg tgatacctaa tttccagggg ctggttggtg gcggcgtcga 8520
tggcttgcaa gaggccgcat ccccgcggcg cgactacggt accgcgcggc gggcggtggg 8580
ccgcgggggt gtccttggat gatgcatcta aaagcggtga cgcgggcgag cccccggagg 8640
tagggggggc tccggacccg ccgggagagg gggcaggggc acgtcggcgc cgcgcgcggg 8700
caggagctgg tgctgcgcgc gtaggttgct ggcgaacgcg acgacgcggc ggttgatctc 8760
ctgaatctgg cgcctctgcg tgaagacgac gggcccggtg agcttgagcc tgaaagagag 8820
ttcgacagaa tcaatttcgg tgtcgttgac ggcggcctgg cgcaaaatct cctgcacgtc 8880
tcctgagttg tcttgatagg cgatctcggc catgaactgc tcgatctctt cctcctggag 8940
atctccgcgt ccggctcgct ccacggtggc ggcgaggtcg ttggaaatgc gggccatgag 9000
ctgcgagaag gcgttgaggc ctccctcgtt ccagacgcgg ctgtagacca cgcccccttc 9060
ggcatcgcgg gcgcgcatga ccacctgcgc gagattgagc tccacgtgcc gggcgaagac 9120
ggcgtagttt cgcaggcgct gaaagaggta gttgagggtg gtggcggtgt gttctgccac 9180
gaagaagtac ataacccagc gtcgcaacgt ggattcgttg atatccccca aggcctcaag 9240
gcgctccatg gcctcgtaga agtccacggc gaagttgaaa aactgggagt tgcgcgccga 9300
cacggttaac tcctcctcca gaagacggat gagctcggcg acagtgtcgc gcacctcgcg 9360
ctcaaaggct acaggggcct cttcttcttc ttcaatctcc tcttccataa gggcctcccc 9420
ttcttcttct tctggcggcg gtgggggagg ggggacacgg cggcgacgac ggcgcaccgg 9480
gaggcggtcg acaaagcgct cgatcatctc cccgcggcga cggcgcatgg tctcggtgac 9540
ggcgcggccg ttctcgcggg ggcgcagttg gaagacgccg cccgtcatgt cccggttatg 9600
ggttggcggg gggctgccat gcggcaggga tacggcgcta acgatgcatc tcaacaattg 9660
ttgtgtaggt actccgccgc cgagggacct gagcgagtcc gcatcgaccg gatcggaaaa 9720
cctctcgaga aaggcgtcta accagtcaca gtcgcaaggt aggctgagca ccgtggcggg 9780
cggcagcggg cggcggtcgg ggttgtttct ggcggaggtg ctgctgatga tgtaattaaa 9840
gtaggcggtc ttgagacggc ggatggtcga cagaagcacc atgtccttgg gtccggcctg 9900
ctgaatgcgc aggcggtcgg ccatgcccca ggcttcgttt tgacatcggc gcaggtcttt 9960
gtagtagtct tgcatgagcc tttctaccgg cacttcttct tctccttcct cttgtcctgc 10020
atctcttgca tctatcgctg cggcggcggc ggagtttggc cgtaggtggc gccctcttcc 10080
tcccatgcgt gtgaccccga agcccctcat cggctgaagc agggctaggt cggcgacaac 10140
gcgctcggct aatatggcct gctgcacctg cgtgagggta gactggaagt catccatgtc 10200
cacaaagcgg tggtatgcgc ccgtgttgat ggtgtaagtg cagttggcca taacggacca 10260
gttaacggtc tggtgacccg gctgcgagag ctcggtgtac ctgagacgcg agtaagccct 10320
cgagtcaaat acgtagtcgt tgcaagtccg caccaggtac tggtatccca ccaaaaagtg 10380
cggcggcggc tggcggtaga ggggccagcg tagggtggcc ggggctccgg gggcgagatc 10440
ttccaacata aggcgatgat atccgtagat gtacctggac atccaggtga tgccggcggc 10500
ggtggtggag gcgcgcggaa agtcgcggac gcggttccag atgttgcgca gcggcaaaaa 10560
gtgctccatg gtcgggacgc tctggccggt caggcgcgcg caatcgttga cgctctagac 10620
cgtgcaaaag gagagcctgt aagcgggcac tcttccgtgg tctggtggat aaattcgcaa 10680
gggtatcatg gcggacgacc ggggttcgag ccccgtatcc ggccgtccgc cgtgatccat 10740
gcggttaccg cccgcgtgtc gaacccaggt gtgcgacgtc agacaacggg ggagtgctcc 10800
ttttggcttc cttccaggcg cggcggctgc tgcgctagct tttttggcca ctggccgcgc 10860
gcagcgtaag cggttaggct ggaaagcgaa agcattaagt ggctcgctcc ctgtagccgg 10920
agggttattt tccaagggtt gagtcgcggg acccccggtt cgagtctcgg accggccgga 10980
ctgcggcgaa cgggggtttg cctccccgtc atgcaagacc ccgcttgcaa attcctccgg 11040
aaacagggac gagccccttt tttgcttttc ccagatgcat ccggtgctgc ggcagatgcg 11100
cccccctcct cagcagcggc aagagcaaga gcagcggcag acatgcaggg caccctcccc 11160
tcctcctacc gcgtcaggag gggcgacatc cgcggttgac gcggcagcag atggtgatta 11220
cgaacccccg cggcgccggg cccggcacta cctggacttg gaggagggcg agggcctggc 11280
gcggctagga gcgccctctc ctgagcggta cccaagggtg cagctgaagc gtgatacgcg 11340
tgaggcgtac gtgccgcggc agaacctgtt tcgcgaccgc gagggagagg agcccgagga 11400
gatgcgggat cgaaagttcc acgcagggcg cgagctgcgg catggcctga atcgcgagcg 11460
gttgctgcgc gaggaggact ttgagcccga cgcgcgaacc gggattagtc ccgcgcgcgc 11520
acacgtggcg gccgccgacc tggtaaccgc atacgagcag acggtgaacc aggagattaa 11580
ctttcaaaaa agctttaaca accacgtgcg tacgcttgtg gcgcgcgagg aggtggctat 11640
aggactgatg catctgtggg actttgtaag cgcgctggag caaaacccaa atagcaagcc 11700
gctcatggcg cagctgttcc ttatagtgca gcacagcagg gacaacgagg cattcaggga 11760
tgcgctgcta aacatagtag agcccgaggg ccgctggctg ctcgatttga taaacatcct 11820
gcagagcata gtggtgcagg agcgcagctt gagcctggct gacaaggtgg ccgccatcaa 11880
ctattccatg cttagcctgg gcaagtttta cgcccgcaag atataccata ccccttacgt 11940
tcccatagac aaggaggtaa agatcgaggg gttctacatg cgcatggcgc tgaaggtgct 12000
taccttgagc gacgacctgg gcgtttatcg caacgagcgc atccacaagg ccgtgagcgt 12060
gagccggcgg cgcgagctca gcgaccgcga gctgatgcac agcctgcaaa gggccctggc 12120
tggcacgggc agcggcgata gagaggccga gtcctacttt gacgcgggcg ctgacctgcg 12180
ctgggcccca agccgacgcg ccctggaggc agctggggcc ggacctgggc tggcggtggc 12240
acccgcgcgc gctggcaacg tcggcggcgt ggaggaatat gacgaggacg atgagtacga 12300
gccagaggac ggcgagtact aagcggtgat gtttctgatc agatgatgca agacgcaacg 12360
gacccggcgg tgcgggcggc gctgcagagc cagccgtccg gccttaactc cacggacgac 12420
tggcgccagg tcatggaccg catcatgtcg ctgactgcgc gcaatcctga cgcgttccgg 12480
cagcagccgc aggccaaccg gctctccgca attctggaag cggtggtccc ggcgcgcgca 12540
aaccccacgc acgagaaggt gctggcgatc gtaaacgcgc tggccgaaaa cagggccatc 12600
cggcccgacg aggccggcct ggtctacgac gcgctgcttc agcgcgtggc tcgttacaac 12660
agcggcaacg tgcagaccaa cctggaccgg ctggtggggg atgtgcgcga ggccgtggcg 12720
cagcgtgagc gcgcgcagca gcagggcaac ctgggctcca tggttgcact aaacgccttc 12780
ctgagtacac agcccgccaa cgtgccgcgg ggacaggagg actacaccaa ctttgtgagc 12840
gcactgcggc taatggtgac tgagacaccg caaagtgagg tgtaccagtc tgggccagac 12900
tattttttcc agaccagtag acaaggcctg cagaccgtaa acctgagcca ggctttcaaa 12960
aacttgcagg ggctgtgggg ggtgcgggct cccacaggcg accgcgcgac cgtgtctagc 13020
ttgctgacgc ccaactcgcg cctgttgctg ctgctaatag cgcccttcac ggacagtggc 13080
agcgtgtccc gggacacata cctaggtcac ttgctgacac tgtaccgcga ggccataggt 13140
caggcgcatg tggacgagca tactttccag gagattacaa gtgtcagccg cgcgctgggg 13200
caggaggaca cgggcagcct ggaggcaacc ctaaactacc tgctgaccaa ccggcggcag 13260
aagatcccct cgttgcacag tttaaacagc gaggaggagc gcattttgcg ctacgtgcag 13320
cagagcgtga gccttaacct gatgcgcgac ggggtaacgc ccagcgtggc gctggacatg 13380
accgcgcgca acatggaacc gggcatgtat gcctcaaacc ggccgtttat caaccgccta 13440
atggactact tgcatcgcgc ggccgccgtg aaccccgagt atttcaccaa tgccatcttg 13500
aacccgcact ggctaccgcc ccctggtttc tacaccgggg gattcgaggt gcccgagggt 13560
aacgatggat tcctctggga cgacatagac gacagcgtgt tttccccgca accgcagacc 13620
ctgctagagt tgcaacagcg cgagcaggca gaggcggcgc tgcgaaagga aagcttccgc 13680
aggccaagca gcttgtccga tctaggcgct gcggccccgc ggtcagatgc tagtagccca 13740
tttccaagct tgatagggtc tcttaccagc actcgcacca cccgcccgcg cctgctgggc 13800
gaggaggagt acctaaacaa ctcgctgctg cagccgcagc gcgaaaaaaa cctgcctccg 13860
gcatttccca acaacgggat agagagccta gtggacaaga tgagtagatg gaagacgtac 13920
gcgcaggagc acagggacgt gccaggcccg cgcccgccca cccgtcgtca aaggcacgac 13980
cgtcagcggg gtctggtgtg ggaggacgat gactcggcag acgacagcag cgtcctggat 14040
ttgggaggga gtggcaaccc gtttgcgcac cttcgcccca ggctggggag aatgttttaa 14100
aaaaaaaaaa gcatgatgca aaataaaaaa ctcaccaagg ccatggcacc gagcgttggt 14160
tttcttgtat tccccttagt atgcggcgcg cggcgatgta tgaggaaggt cctcctccct 14220
cctacgagag tgtggtgagc gcggcgccag tggcggcggc gctgggttct cccttcgatg 14280
ctcccctgga cccgccgttt gtgcctccgc ggtacctgcg gcctaccggg gggagaaaca 14340
gcatccgtta ctctgagttg gcacccctat tcgacaccac ccgtgtgtac ctggtggaca 14400
acaagtcaac ggatgtggca tccctgaact accagaacga ccacagcaac tttctgacca 14460
cggtcattca aaacaatgac tacagcccgg gggaggcaag cacacagacc atcaatcttg 14520
acgaccggtc gcactggggc ggcgacctga aaaccatcct gcataccaac atgccaaatg 14580
tgaacgagtt catgtttacc aataagttta aggcgcgggt gatggtgtcg cgcttgccta 14640
ctaaggacaa tcaggtggag ctgaaatacg agtgggtgga gttcacgctg cccgagggca 14700
actactccga gaccatgacc atagacctta tgaacaacgc gatcgtggag cactacttga 14760
aagtgggcag acagaacggg gttctggaaa gcgacatcgg ggtaaagttt gacacccgca 14820
acttcagact ggggtttgac cccgtcactg gtcttgtcat gcctggggta tatacaaacg 14880
aagccttcca tccagacatc attttgctgc caggatgcgg ggtggacttc acccacagcc 14940
gcctgagcaa cttgttgggc atccgcaagc ggcaaccctt ccaggagggc tttaggatca 15000
cctacgatga tctggagggt ggtaacattc ccgcactgtt ggatgtggac gcctaccagg 15060
cgagcttgaa agatgacacc gaacagggcg ggggtggcgc aggcggcagc aacagcagtg 15120
gcagcggcgc ggaagagaac tccaacgcgg cagccgcggc aatgcagccg gtggaggaca 15180
tgaacgatca tgccattcgc ggcgacacct ttgccacacg ggctgaggag aagcgcgctg 15240
aggccgaagc agcggccgaa gctgccgccc ccgctgcgca acccgaggtc gagaagcctc 15300
agaagaaacc ggtgatcaaa cccctgacag aggacagcaa gaaacgcagt tacaacctaa 15360
taagcaatga cagcaccttc acccagtacc gcagctggta ccttgcatac aactacggcg 15420
accctcagac cggaatccgc tcatggaccc tgctttgcac tcctgacgta acctgcggct 15480
cggagcaggt ctactggtcg ttgccagaca tgatgcaaga ccccgtgacc ttccgctcca 15540
cgcgccagat cagcaacttt ccggtggtgg gcgccgagct gttgcccgtg cactccaaga 15600
gcttctacaa cgaccaggcc gtctactccc aactcatccg ccagtttacc tctctgaccc 15660
acgtgttcaa tcgctttccc gagaaccaga ttttggcgcg cccgccagcc cccaccatca 15720
ccaccgtcag tgaaaacgtt cctgctctca cagatcacgg gacgctaccg ctgcgcaaca 15780
gcatcggagg agtccagcga gtgaccatta ctgacgccag acgccgcacc tgcccctacg 15840
tttacaaggc cctgggcata gtctcgccgc gcgtcctatc gagccgcact ttttgagcaa 15900
gcatgtccat ccttatatcg cccagcaata acacaggctg gggcctgcgc ttcccaagca 15960
agatgtttgg cggggccaag aagcgctccg accaacaccc agtgcgcgtg cgcgggcact 16020
accgcgcgcc ctggggcgcg cacaaacgcg gccgcactgg gcgcaccacc gtcgatgacg 16080
ccatcgacgc ggtggtggag gaggcgcgca actacacgcc cacgccgcca ccagtgtcca 16140
cagtggacgc ggccattcag accgtggtgc gcggagcccg gcgctatgct aaaatgaaga 16200
gacggcggag gcgcgtagca cgtcgccacc gccgccgacc cggcactgcc gcccaacgcg 16260
cggcggcggc cctgcttaac cgcgcacgtc gcaccggccg acgggcggcc atgcgggccg 16320
ctcgaaggct ggccgcgggt attgtcactg tgccccccag gtccaggcga cgagcggccg 16380
ccgcagcagc cgcggccatt agtgctatga ctcagggtcg caggggcaac gtgtattggg 16440
tgcgcgactc ggttagcggc ctgcgcgtgc ccgtgcgcac ccgccccccg cgcaactaga 16500
ttgcaagaaa aaactactta gactcgtact gttgtatgta tccagcggcg gcggcgcgca 16560
acgaagctat gtccaagcgc aaaatcaaag aagagatgct ccaggtcatc gcgccggaga 16620
tctatggccc cccgaagaag gaagagcagg attacaagcc ccgaaagcta aagcgggtca 16680
aaaagaaaaa gaaagatgat gatgatgaac ttgacgacga ggtggaactg ctgcacgcta 16740
ccgcgcccag gcgacgggta cagtggaaag gtcgacgcgt aaaacgtgtt ttgcgacccg 16800
gcaccaccgt agtctttacg cccggtgagc gctccacccg cacctacaag cgcgtgtatg 16860
atgaggtgta cggcgacgag gacctgcttg agcaggccaa cgagcgcctc ggggagtttg 16920
cctacggaaa gcggcataag gacatgctgg cgttgccgct ggacgagggc aacccaacac 16980
ctagcctaaa gcccgtaaca ctgcagcagg tgctgcccgc gcttgcaccg tccgaagaaa 17040
agcgcggcct aaagcgcgag tctggtgact tggcacccac cgtgcagctg atggtaccca 17100
agcgccagcg actggaagat gtcttggaaa aaatgaccgt ggaacctggg ctggagcccg 17160
aggtccgcgt gcggccaatc aagcaggtgg cgccgggact gggcgtgcag accgtggacg 17220
ttcagatacc cactaccagt agcaccagta ttgccaccgc cacagagggc atggagacac 17280
aaacgtcccc ggttgcctca gcggtggcgg atgccgcggt gcaggcggtc gctgcggccg 17340
cgtccaagac ctctacggag gtgcaaacgg acccgtggat gtttcgcgtt tcagcccccc 17400
ggcgcccgcg cggttcgagg aagtacggcg ccgccagcgc gctactgccc gaatatgccc 17460
tacatccttc cattgcgcct acccccggct atcgtggcta cacctaccgc cccagaagac 17520
gagcaactac ccgacgccga accaccactg gaacccgccg ccgccgtcgc cgtcgccagc 17580
ccgtgctggc cccgatttcc gtgcgcaggg tggctcgcga aggaggcagg accctggtgc 17640
tgccaacagc gcgctaccac cccagcatcg tttaaaagcc ggtctttgtg gttcttgcag 17700
atatggccct cacctgccgc ctccgtttcc cggtgccggg attccgagga agaatgcacc 17760
gtaggagggg catggccggc cacggcctga cgggcggcat gcgtcgtgcg caccaccggc 17820
ggcggcgcgc gtcgcaccgt cgcatgcgcg gcggtatcct gcccctcctt attccactga 17880
tcgccgcggc gattggcgcc gtgcccggaa ttgcatccgt ggccttgcag gcgcagagac 17940
actgattaaa aacaagttgc atgtggaaaa atcaaaataa aaagtctgga ctctcacgct 18000
cgcttggtcc tgtaactatt ttgtagaatg gaagacatca actttgcgtc tctggccccg 18060
cgacacggct cgcgcccgtt catgggaaac tggcaagata tcggcaccag caatatgagc 18120
ggtggcgcct tcagctgggg ctcgctgtgg agcggcatta aaaatttcgg ttccaccgtt 18180
aagaactatg gcagcaaggc ctggaacagc agcacaggcc agatgctgag ggataagttg 18240
aaagagcaaa atttccaaca aaaggtggta gatggcctgg cctctggcat tagcggggtg 18300
gtggacctgg ccaaccaggc agtgcaaaat aagattaaca gtaagcttga tccccgccct 18360
cccgtagagg agcctccacc ggccgtggag acagtgtctc cagaggggcg tggcgaaaag 18420
cgtccgcgcc ccgacaggga agaaactctg gtgacgcaaa tagacgagcc tccctcgtac 18480
gaggaggcac taaagcaagg cctgcccacc acccgtccca tcgcgcccat ggctaccgga 18540
gtgctgggcc agcacacacc cgtaacgctg gacctgcctc cccccgccga cacccagcag 18600
aaacctgtgc tgccaggccc gaccgccgtt gttgtaaccc gtcctagccg cgcgtccctg 18660
cgccgcgccg ccagcggtcc gcgatcgttg cggcccgtag ccagtggcaa ctggcaaagc 18720
acactgaaca gcatcgtggg tctgggggtg caatccctga agcgccgacg atgcttctga 18780
atagctaacg tgtcgtatgt gtgtcatgta tgcgtccatg tcgccgccag aggagctgct 18840
gagccgccgc gcgcccgctt tccaagatgg ctaccccttc gatgatgccg cagtggtctt 18900
acatgcacat ctcgggccag gacgcctcgg agtacctgag ccccgggctg gtgcagtttg 18960
cccgcgccac cgagacgtac ttcagcctga ataacaagtt tagaaacccc acggtggcgc 19020
ctacgcacga cgtgaccaca gaccggtccc agcgtttgac gctgcggttc atccctgtgg 19080
accgtgagga tactgcgtac tcgtacaagg cgcggttcac cctagctgtg ggtgataacc 19140
gtgtgctgga catggcttcc acgtactttg acatccgcgg cgtgctggac aggggcccta 19200
cttttaagcc ctactctggc actgcctaca acgccctggc tcccaagggt gccccaaatc 19260
cttgcgaatg ggatgaagct gctactgctc ttgaaataaa cctagaagaa gaggacgatg 19320
acaacgaaga cgaagtagac gagcaagctg agcagcaaaa aactcacgta tttgggcagg 19380
cgccttattc tggtataaat attacaaagg agggtattca aataggtgtc gaaggtcaaa 19440
cacctaaata tgccgataaa acatttcaac ctgaacctca aataggagaa tctcagtggt 19500
acgaaactga aattaatcat gcagctggga gagtccttaa aaagactacc ccaatgaaac 19560
catgttacgg ttcatatgca aaacccacaa atgaaaatgg agggcaaggc attcttgtaa 19620
agcaacaaaa tggaaagcta gaaagtcaag tggaaatgca atttttctca actactgagg 19680
cgaccgcagg caatggtgat aacttgactc ctaaagtggt attgtacagt gaagatgtag 19740
atatagaaac cccagacact catatttctt acatgcccac tattaaggaa ggtaactcac 19800
gagaactaat gggccaacaa tctatgccca acaggcctaa ttacattgct tttagggaca 19860
attttattgg tctaatgtat tacaacagca cgggtaatat gggtgttctg gcgggccaag 19920
catcgcagtt gaatgctgtt gtagatttgc aagacagaaa cacagagctt tcataccagc 19980
ttttgcttga ttccattggt gatagaacca ggtacttttc tatgtggaat caggctgttg 20040
acagctatga tccagatgtt agaattattg aaaatcatgg aactgaagat gaacttccaa 20100
attactgctt tccactggga ggtgtgatta atacagagac tcttaccaag gtaaaaccta 20160
aaacaggtca ggaaaatgga tgggaaaaag atgctacaga attttcagat aaaaatgaaa 20220
taagagttgg aaataatttt gccatggaaa tcaatctaaa tgccaacctg tggagaaatt 20280
tcctgtactc caacatagcg ctgtatttgc ccgacaagct aaagtacagt ccttccaacg 20340
taaaaatttc tgataaccca aacacctacg actacatgaa caagcgagtg gtggctcccg 20400
ggttagtgga ctgctacatt aaccttggag cacgctggtc ccttgactat atggacaacg 20460
tcaacccatt taaccaccac cgcaatgctg gcctgcgcta ccgctcaatg ttgctgggca 20520
atggtcgcta tgtgcccttc cacatccagg tgcctcagaa gttctttgcc attaaaaacc 20580
tccttctcct gccgggctca tacacctacg agtggaactt caggaaggat gttaacatgg 20640
ttctgcagag ctccctagga aatgacctaa gggttgacgg agccagcatt aagtttgata 20700
gcatttgcct ttacgccacc ttcttcccca tggcccacaa caccgcctcc acgcttgagg 20760
ccatgcttag aaacgacacc aacgaccagt cctttaacga ctatctctcc gccgccaaca 20820
tgctctaccc tatacccgcc aacgctacca acgtgcccat atccatcccc tcccgcaact 20880
gggcggcttt ccgcggctgg gccttcacgc gccttaagac taaggaaacc ccatcactgg 20940
gctcgggcta cgacccttat tacacctact ctggctctat accctaccta gatggaacct 21000
tttacctcaa ccacaccttt aagaaggtgg ccattacctt tgactcttct gtcagctggc 21060
ctggcaatga ccgcctgctt acccccaacg agtttgaaat taagcgctca gttgacgggg 21120
agggttacaa cgttgcccag tgtaacatga ccaaagactg gttcctggta caaatgctag 21180
ctaactacaa cattggctac cagggcttct atatcccaga gagctacaag gaccgcatgt 21240
actccttctt tagaaacttc cagcccatga gccgtcaggt ggtggatgat actaaataca 21300
aggactacca acaggtgggc atcctacacc aacacaacaa ctctggattt gttggctacc 21360
ttgcccccac catgcgcgaa ggacaggcct accctgctaa cttcccctat ccgcttatag 21420
gcaagaccgc agttgacagc attacccaga aaaagtttct ttgcgatcgc accctttggc 21480
gcatcccatt ctccagtaac tttatgtcca tgggcgcact cacagacctg ggccaaaacc 21540
ttctctacgc caactccgcc cacgcgctag acatgacttt tgaggtggat cccatggacg 21600
agcccaccct tctttatgtt ttgtttgaag tctttgacgt ggtccgtgtg caccggccgc 21660
accgcggcgt catcgaaacc gtgtacctgc gcacgccctt ctcggccggc aacgccacaa 21720
cataaagaag caagcaacat caacaacagc tgccgccatg ggctccagtg agcaggaact 21780
gaaagccatt gtcaaagatc ttggttgtgg gccatatttt ttgggcacct atgacaagcg 21840
ctttccaggc tttgtttctc cacacaagct cgcctgcgcc atagtcaata cggccggtcg 21900
cgagactggg ggcgtacact ggatggcctt tgcctggaac ccgcactcaa aaacatgcta 21960
cctctttgag ccctttggct tttctgacca gcgactcaag caggtttacc agtttgagta 22020
cgagtcactc ctgcgccgta gcgccattgc ttcttccccc gaccgctgta taacgctgga 22080
aaagtccacc caaagcgtac aggggcccaa ctcggccgcc tgtggactat tctgctgcat 22140
gtttctccac gcctttgcca actggcccca aactcccatg gatcacaacc ccaccatgaa 22200
ccttattacc ggggtaccca actccatgct caacagtccc caggtacagc ccaccctgcg 22260
tcgcaaccag gaacagctct acagcttcct ggagcgccac tcgccctact tccgcagcca 22320
cagtgcgcag attaggagcg ccacttcttt ttgtcacttg aaaaacatgt aaaaataatg 22380
tactagagac actttcaata aaggcaaatg cttttatttg tacactctcg ggtgattatt 22440
tacccccacc cttgccgtct gcgccgttta aaaatcaaag gggttctgcc gcgcatcgct 22500
atgcgccact ggcagggaca cgttgcgata ctggtgttta gtgctccact taaactcagg 22560
cacaaccatc cgcggcagct cggtgaagtt ttcactccac aggctgcgca ccatcaccaa 22620
cgcgtttagc aggtcgggcg ccgatatctt gaagtcgcag ttggggcctc cgccctgcgc 22680
gcgcgagttg cgatacacag ggttgcagca ctggaacact atcagcgccg ggtggtgcac 22740
gctggccagc acgctcttgt cggagatcag atccgcgtcc aggtcctccg cgttgctcag 22800
ggcgaacgga gtcaactttg gtagctgcct tcccaaaaag ggcgcgtgcc caggctttga 22860
gttgcactcg caccgtagtg gcatcaaaag gtgaccgtgc ccggtctggg cgttaggata 22920
cagcgcctgc ataaaagcct tgatctgctt aaaagccacc tgagcctttg cgccttcaga 22980
gaagaacatg ccgcaagact tgccggaaaa ctgattggcc ggacaggccg cgtcgtgcac 23040
gcagcacctt gcgtcggtgt tggagatctg caccacattt cggccccacc ggttcttcac 23100
gatcttggcc ttgctagact gctccttcag cgcgcgctgc ccgttttcgc tcgtcacatc 23160
catttcaatc acgtgctcct tatttatcat aatgcttccg tgtagacact taagctcgcc 23220
ttcgatctca gcgcagcggt gcagccacaa cgcgcagccc gtgggctcgt gatgcttgta 23280
ggtcacctct gcaaacgact gcaggtacgc ctgcaggaat cgccccatca tcgtcacaaa 23340
ggtcttgttg ctggtgaagg tcagctgcaa cccgcggtgc tcctcgttca gccaggtctt 23400
gcatacggcc gccagagctt ccacttggtc aggcagtagt ttgaagttcg cctttagatc 23460
gttatccacg tggtacttgt ccatcagcgc gcgcgcagcc tccatgccct tctcccacgc 23520
agacacgatc ggcacactca gcgggttcat caccgtaatt tcactttccg cttcgctggg 23580
ctcttcctct tcctcttgcg tccgcatacc acgcgccact gggtcgtctt cattcagccg 23640
ccgcactgtg cgcttacctc ctttgccatg cttgattagc accggtgggt tgctgaaacc 23700
caccatttgt agcgccacat cttctctttc ttcctcgctg tccacgatta cctctggtga 23760
tggcgggcgc tcgggcttgg gagaagggcg cttctttttc ttcttgggcg caatggccaa 23820
atccgccgcc gaggtcgatg gccgcgggct gggtgtgcgc ggcaccagcg cgtcttgtga 23880
tgagtcttcc tcgtcctcgg actcgatacg ccgcctcatc cgcttttttg ggggcgcccg 23940
gggaggcggc ggcgacgggg acggggacga cacgtcctcc atggttgggg gacgtcgcgc 24000
cgcaccgcgt ccgcgctcgg gggtggtttc gcgctgctcc tcttcccgac tggccatttc 24060
cttctcctat aggcagaaaa agatcatgga gtcagtcgag aagaaggaca gcctaaccgc 24120
cccctctgag ttcgccacca ccgcctccac cgatgccgcc aacgcgccta ccaccttccc 24180
cgtcgaggca cccccgcttg aggaggagga agtgattatc gagcaggacc caggttttgt 24240
aagcgaagac gacgaggacc gctcagtacc aacagaggat aaaaagcaag accaggacaa 24300
cgcagaggca aacgaggaac aagtcgggcg gggggacgaa aggcatggcg actacctaga 24360
tgtgggagac gacgtgctgt tgaagcatct gcagcgccag tgcgccatta tctgcgacgc 24420
gttgcaagag cgcagcgatg tgcccctcgc catagcggat gtcagccttg cctacgaacg 24480
ccacctattc tcaccgcgcg taccccccaa acgccaagaa aacggcacat gcgagcccaa 24540
cccgcgcctc aacttctacc ccgtatttgc cgtgccagag gtgcttgcca cctatcacat 24600
ctttttccaa aactgcaaga tacccctatc ctgccgtgcc aaccgcagcc gagcggacaa 24660
gcagctggcc ttgcggcagg gcgctgtcat acctgatatc gcctcgctca acgaagtgcc 24720
aaaaatcttt gagggtcttg gacgcgacga gaagcgcgcg gcaaacgctc tgcaacagga 24780
aaacagcgaa aatgaaagtc actctggagt gttggtggaa ctcgagggtg acaacgcgcg 24840
cctagccgta ctaaaacgca gcatcgaggt cacccacttt gcctacccgg cacttaacct 24900
accccccaag gtcatgagca cagtcatgag tgagctgatc gtgcgccgtg cgcagcccct 24960
ggagagggat gcaaatttgc aagaacaaac agaggagggc ctacccgcag ttggcgacga 25020
gcagctagcg cgctggcttc aaacgcgcga gcctgccgac ttggaggagc gacgcaaact 25080
aatgatggcc gcagtgctcg ttaccgtgga gcttgagtgc atgcagcggt tctttgctga 25140
cccggagatg cagcgcaagc tagaggaaac attgcactac acctttcgac agggctacgt 25200
acgccaggcc tgcaagatct ccaacgtgga gctctgcaac ctggtctcct accttggaat 25260
tttgcacgaa aaccgccttg ggcaaaacgt gcttcattcc acgctcaagg gcgaggcgcg 25320
ccgcgactac gtccgcgact gcgtttactt atttctatgc tacacctggc agacggccat 25380
gggcgtttgg cagcagtgct tggaggagtg caacctcaag gagctgcaga aactgctaaa 25440
gcaaaacttg aaggacctat ggacggcctt caacgagcgc tccgtggccg cgcacctggc 25500
ggacatcatt ttccccgaac gcctgcttaa aaccctgcaa cagggtctgc cagacttcac 25560
cagtcaaagc atgttgcaga actttaggaa ctttatccta gagcgctcag gaatcttgcc 25620
cgccacctgc tgtgcacttc ctagcgactt tgtgcccatt aagtaccgcg aatgccctcc 25680
gccgctttgg ggccactgct accttctgca gctagccaac taccttgcct accactctga 25740
cataatggaa gacgtgagcg gtgacggtct actggagtgt cactgtcgct gcaacctatg 25800
caccccgcac cgctccctgg tttgcaattc gcagctgctt aacgaaagtc aaattatcgg 25860
tacctttgag ctgcagggtc cctcgcctga cgaaaagtcc gcggctccgg ggttgaaact 25920
cactccgggg ctgtggacgt cggcttacct tcgcaaattt gtacctgagg actaccacgc 25980
ccacgagatt aggttctacg aagaccaatc ccgcccgcca aatgcggagc ttaccgcctg 26040
cgtcattacc cagggccaca ttcttggcca attgcaagcc atcaacaaag cccgccaaga 26100
gtttctgcta cgaaagggac ggggggttta cttggacccc cagtccggcg aggagctcaa 26160
cccaatcccc ccgccgccgc agccctatca gcagcagccg cgggcccttg cttcccagga 26220
tggcacccaa aaagaagctg cagctgccgc cgccacccac ggacgaggag gaatactggg 26280
acagtcaggc agaggaggtt ttggacgagg aggaggagga catgatggaa gactgggaga 26340
gcctagacga ggaagcttcc gaggtcgaag aggtgtcaga cgaaacaccg tcaccctcgg 26400
tcgcattccc ctcgccggcg ccccagaaat cggcaaccgg ttccagcatg gctacaacct 26460
ccgctcctca ggcgccgccg gcactgcccg ttcgccgacc caaccgtaga tgggacacca 26520
ctggaaccag ggccggtaag tccaagcagc cgccgccgtt agcccaagag caacaacagc 26580
gccaaggcta ccgctcatgg cgcgggcaca agaacgccat agttgcttgc ttgcaagact 26640
gtgggggcaa catctccttc gcccgccgct ttcttctcta ccatcacggc gtggccttcc 26700
cccgtaacat cctgcattac taccgtcatc tctacagccc atactgcacc ggcggcagcg 26760
gcagcggcag caacagcagc ggccacacag aagcaaaggc gaccggatag caagactctg 26820
acaaagccca agaaatccac agcggcggca gcagcaggag gaggagcgct gcgtctggcg 26880
cccaacgaac ccgtatcgac ccgcgagctt agaaacagga tttttcccac tctgtatgct 26940
atatttcaac agagcagggg ccaagaacaa gagctgaaaa taaaaaacag gtctctgcga 27000
tccctcaccc gcagctgcct gtatcacaaa agcgaagatc agcttcggcg cacgctggaa 27060
gacgcggagg ctctcttcag taaatactgc gcgctgactc ttaaggacta gtttcgcgcc 27120
ctttctcaaa tttaagcgcg aaaactacgt catctccagc ggccacaccc ggcgccagca 27180
cctgtcgtca gcgccatttc aactttgtat acaaaagttg tgatgagcaa ggaaattccc 27240
acgccctaca tgtggagtta ccagccacaa atgggacttg cggctggagc tgcccaagac 27300
tactcaaccc gaataaacta catgagcgcg ggaccccaca tgatatcccg ggtcaacgga 27360
atacgcgccc accgaaaccg aattctcctg gaacaggcgg ctattaccac cacacctcgt 27420
aataacctta atccccgtag ttggcccgct gccctggtgt accaggaaag tcccgctccc 27480
accactgtgg tacttcccag agacgcccag gccgaagttc agatgactaa ctcaggggcg 27540
cagcttgcgg gcggctttcg tcacagggtg cggtcgcccg ggcagggtat aactcacctg 27600
acaatcagag ggcgaggtat tcagctcaac gacgagtcgg tgagctcctc gcttggtctc 27660
cgtccggacg ggacatttca gatcggcggc gccggccgct cttcattcac gcctcgtcag 27720
gcaatcctaa ctctgcagac ctcgtcctct gagccgcgct ctggaggcat tggaactctg 27780
caatttattg aggagtttgt gccatcggtc tactttaacc ccttctcggg acctcccggc 27840
cactatccgg atcaatttat tcctaacttt gacgcggtaa aggactcggc ggacggctac 27900
gactgaatgt taagtggaga ggcagagcaa ctgcgcctga aacacctggt ccactgtcgc 27960
cgccacaagt gctttgcccg cgactccggt gagttttgct actttgaatt gcccgaggat 28020
catatcgagg gcccggcgca cggcgtccgg cttaccgccc agggagagct tgcccgtagc 28080
ctgattcggg agtttaccca gcgccccctg ctagttgagc gggacagggg accctgtgtt 28140
ctcactgtga tttgcaactg tcctaaccct ggattacatc aagatctttg ttgccatctc 28200
tgtgctgagt ataataaata cagaaattaa aatatactgg ggctcctatc gccatcctgt 28260
aaacgccacc gtcttcaccc gcccaagcaa accaaggcga accttacctg gtacttttaa 28320
catctctccc tctgtgattt acaacagttt caacccagac ggagtgagtc tacgagagaa 28380
cctctccgag ctcagctact ccatcagaaa aaacaccacc ctccttacct gccgggaacg 28440
tacgagtgcg tcaccggccg ctgcaccaca cctaccgcct gaccgtaaac cagacttttt 28500
ccggacagac ctcaataact ctgtttacca gaacaggagg tgagcttaga aaacccttag 28560
ggtattaggc caaaggcgca gctactgtgg ggtttatgaa caattcaagc aactctacgg 28620
gctattctaa ttcaggtttc tctagaatcg gggttggggt tattctctgt cttgtgattc 28680
tctttattct tatactaacg cttctctgcc taaggctcgc cgcctgctgt gtgcacattt 28740
gcatttattg tcagcttttt aaacgctggg gtcgccaccc aagatgatta ggtacataat 28800
cctaggttta ctcacccttg cgtcagccca cggtaccacc caaaaggtgg attttaagga 28860
gccagcctgt aatgttacat tcgcagctga agctaatgag tgcaccactc ttataaaatg 28920
caccacagaa catgaaaagc tgcttattcg ccacaaaaac aaaattggca agtatgctgt 28980
ttatgctatt tggcagccag gtgacactac agagtataat gttacagttt tccagggtaa 29040
aagtcataaa acttttatgt atacttttcc attttatgaa atgtgcgaca ttaccatgta 29100
catgagcaaa cagtataagt tgtggccccc acaaaattgt gtggaaaaca ctggcacttt 29160
ctgctgcact gctatgctaa ttacagtgct cgctttggtc tgtaccctac tctatattaa 29220
atacaaaagc agacgcagct ttattgagga aaagaaaatg ccttaattta ctaagttaca 29280
aagctaatgt caccactaac tgctttactc gctgcttgca aaacaaattc aaaaagttag 29340
cattataatt agaataggat ttaaaccccc cggtcatttc ctgctcaata ccattcccct 29400
gaacaattga ctctatgtgg gatatgctcc agcgctacaa ccttgaagtc aggcttcctg 29460
gatgtcagca tctgactttg gccagcacct gtcccgcgga tttgttccag tccaactaca 29520
gcgacccacc ctaacagaga tgaccaacac aaccaacgcg gccgccgcta ccggacttac 29580
atctaccaca aatacacccc aagtttctgc ctttgtcaat aactgggata acttgggcat 29640
gtggtggttc tccatagcgc ttatgtttgt atgccttatt attatgtggc tcatctgctg 29700
cctaaagcgc aaacgcgccc gaccacccat ctatagtccc atcattgtgc tacacccaaa 29760
caatgatgga atccatagat tggacggact gaaacacatg ttcttttctc ttacagtatg 29820
attaaatgag acatgattcc tcgagttttt atattactga cccttgttgc gcttttttgt 29880
gcgtgctcca cattggctgc ggtttctcac atcgaagtag actgcattcc agccttcaca 29940
gtctatttgc tttacggatt tgtcaccctc acgctcatct gcagcctcat cactgtggtc 30000
atcgccttta tccagtgcat tgactgggtc tgtgtgcgct ttgcatatct cagacaccat 30060
ccccagtaca gggacaggac tatagctgag cttcttagaa ttctttaatt atgaaattta 30120
ctgtgacttt tctgctgatt atttgcaccc tatctgcgtt ttgttccccg acctccaagc 30180
ctcaaagaca tatatcatgc agattcactc gtatatggaa tattccaagt tgctacaatg 30240
aaaaaagcga tctttccgaa gcctggttat atgcaatcat ctctgttatg gtgttctgca 30300
gtaccatctt agccctagct atatatccct accttgacat tggctggaac gcaatagatg 30360
ccatgaacca cccaactttc cccgcgcccg ctatgcttcc actgcaacaa gttgttgccg 30420
gcggctttgt cccagccaat cagcctcgcc caccttctcc cacccccact gaaatcagct 30480
actttaatct aacaggagga gatgactgac accctagatc tagaaatgga cggaattatt 30540
acagagcagc gcctgctaga aagacgcagg gcagcggccg agcaacagcg catgaatcaa 30600
gagctccaag acatggttaa cttgcaccag tgcaaaaggg gtatcttttg tctggtaaag 30660
caggccaaag tcacctacga cagtaatacc accggacacc gccttagcta caagttgcca 30720
accaagcgtc agaaattggt ggtcatggtg ggagaaaagc ccattaccat aactcagcac 30780
tcggtagaaa ccgaaggctg cattcactca ccttgtcaag gacctgagga tctctgcacc 30840
cttattaaga ccctgtgcgg tctcaaagat cttattccct ttaactaata aaaaaaaata 30900
ataaagcatc acttacttaa aatcagttag caaatttctg tccagtttat tcagcagcac 30960
ctccttgccc tcctcccagc tctggtattg cagcttcctc ctggctgcaa actttctcca 31020
caatctaaat ggaatgtcag tttcctcctg ttcctgtcca tccgcaccca ctatcttcat 31080
gttgttgcag atgaagcgcg caagaccgtc tgaagatacc ttcaaccccg tgtatccata 31140
tgacacggaa accggtcctc caactgtgcc ttttcttact cctccctttg tatcccccaa 31200
tgggtttcaa gagagtcccc ctggggtact ctctttgcgc ctatccgaac ctctagttac 31260
ctccaatggc atgcttgcgc tcaaaatggg caacggcctc tctctggacg aggccggcaa 31320
ccttacctcc caaaatgtaa ccactgtgag cccacctctc aaaaaaacca agtcaaacat 31380
aaacctggaa atatctgcac ccctcacagt tacctcagaa gccctaactg tggctgccgc 31440
cgcacctcta atggtcgcgg gcaacacact caccatgcaa tcacaggccc cgctaaccgt 31500
gcacgactcc aaacttagca ttgccaccca aggacccctc acagtgtcag aaggaaagct 31560
agccctgcaa acatcaggcc ccctcaccac caccgatagc agtaccctta ctatcactgc 31620
ctcaccccct ctaactactg ccactggtag cttgggcatt gacttgaaag agcccattta 31680
tacacaaaat ggaaaactag gactaaagta cggggctcct ttgcatgtaa cagacgacct 31740
aaacactttg accgtagcaa ctggtccagg tgtgactatt aataatactt ccttgcaaac 31800
taaagttact ggagccttgg gttttgattc acaaggcaat atgcaactta atgtagcagg 31860
aggactaagg attgattctc aaaacagacg ccttatactt gatgttagtt atccgtttga 31920
tgctcaaaac caactaaatc taagactagg acagggccct ctttttataa actcagccca 31980
caacttggat attaactaca acaaaggcct ttacttgttt acagcttcaa acaattccaa 32040
aaagcttgag gttaacctaa gcactgccaa ggggttgatg tttgacgcta cagccatagc 32100
cattaatgca ggagatgggc ttgaatttgg ttcacctaat gcaccaaaca caaatcccct 32160
caaaacaaaa attggccatg gcctagaatt tgattcaaac aaggctatgg ttcctaaact 32220
aggaactggc cttagttttg acagcacagg tgccattaca gtaggaaaca aaaataatga 32280
taagctaact ttgtggacca caccagctcc atctcctaac tgtagactaa atgcagagaa 32340
agatgctaaa ctcactttgg tcttaacaaa atgtggcagt caaatacttg ctacagtttc 32400
agttttggct gttaaaggca gtttggctcc aatatctgga acagttcaaa gtgctcatct 32460
tattataaga tttgacgaaa atggagtgct actaaacaat tccttcctgg acccagaata 32520
ttggaacttt agaaatggag atcttactga aggcacagcc tatacaaacg ctgttggatt 32580
tatgcctaac ctatcagctt atccaaaatc tcacggtaaa actgccaaaa gtaacattgt 32640
cagtcaagtt tacttaaacg gagacaaaac taaacctgta acactaacca ttacactaaa 32700
cggtacacag gaaacaggag acacaactcc aagtgcatac tctatgtcat tttcatggga 32760
ctggtctggc cacaactaca ttaatgaaat atttgccaca tcctcttaca ctttttcata 32820
cattgcccaa gaataaagaa tcgtttgtgt tatgtttcaa cgtgtttatt tttcaattgc 32880
agaaaatttc aagtcatttt tcattcagta gtatagcccc accaccacat agcttataca 32940
gatcaccgta cctcaacttt gtataataaa gttgaatcaa actcacagaa ccctagtatt 33000
caacctgcca cctccctccc aacacacaga gtacacagtc ctttctcccc ggctggcctt 33060
aaaaagcatc atatcatggg taacagacat attcttaggt gttatattcc acacggtttc 33120
ctgtcgagcc aaacgctcat cagtgatatt aataaactcc ccgggcagct cacttaagtt 33180
catgtcgctg tccagctgct gagccacagg ctgctgtcca acttgcggtt gcttaacggg 33240
cggcgaagga gaagtccacg cctacatggg ggtagagtca taatcgtgca tcaggatagg 33300
gcggtggtgc tgcagcagcg cgcgaataaa ctgctgccgc cgccgctccg tcctgcagga 33360
atacaacatg gcagtggtct cctcagcgat gattcgcacc gcccgcagca taaggcgcct 33420
tgtcctccgg gcacagcagc gcaccctgat ctcacttaaa tcagcacagt aactgcagca 33480
cagcaccaca atattgttca aaatcccaca gtgcaaggcg ctgtatccaa agctcatggc 33540
ggggaccaca gaacccacgt ggccatcata ccacaagcgc aggtagatta agtggcgacc 33600
cctcataaac acgctggaca taaacattac ctcttttggc atgttgtaat tcaccacctc 33660
ccggtaccat ataaacctct gattaaacat ggcgccatcc accaccatcc taaaccagct 33720
ggccaaaacc tgcccgccgg ctatacactg cagggaaccg ggactggaac aatgacagtg 33780
gagagcccag gactcgtaac catggatcat catgctcgtc atgatatcaa tgttggcaca 33840
acacaggcac acgtgcatac acttcctcag gattacaagc tcctcccgcg ttagaaccat 33900
atcccaggga acaacccatt cctgaatcag cgtaaatccc acactgcagg gaagacctcg 33960
cacgtaactc acgttgtgca ttgtcaaagt gttacattcg ggcagcagcg gatgatcctc 34020
cagtatggta gcgcgggttt ctgtctcaaa aggaggtaga cgatccctac tgtacggagt 34080
gcgccgagac aaccgagatc gtgttggtcg tagtgtcatg ccaaatggaa cgccggacgt 34140
agtcatattt cctgaagcaa aaccaggtgc gggcgtgaca aacagatctg cgtctccggt 34200
ctcgccgctt agatcgctct gtgtagtagt tgtagtatat ccactctctc aaagcatcca 34260
ggcgccccct ggcttcgggt tctatgtaaa ctccttcatg cgccgctgcc ctgataacat 34320
ccaccaccgc agaataagcc acacccagcc aacctacaca ttcgttctgc gagtcacaca 34380
cgggaggagc gggaagagct ggaagaacca tgtttttttt tttattccaa aagattatcc 34440
aaaacctcaa aatgaagatc tattaagtga acgcgctccc ctccggtggc gtggtcaaac 34500
tctacagcca aagaacagat aatggcattt gtaagatgtt gcacaatggc ttccaaaagg 34560
caaacggccc tcacgtccaa gtggacgtaa aggctaaacc cttcagggtg aatctcctct 34620
ataaacattc cagcaccttc aaccatgccc aaataattct catctcgcca ccttctcaat 34680
atatctctaa gcaaatcccg aatattaagt ccggccattg taaaaatctg ctccagagcg 34740
ccctccacct tcagcctcaa gcagcgaatc atgattgcaa aaattcaggt tcctcacaga 34800
cctgtataag attcaaaagc ggaacattaa caaaaatacc gcgatcccgt aggtcccttc 34860
gcagggccag ctgaacataa tcgtgcaggt ctgcacggac cagcgcggcc acttccccgc 34920
caggaaccat gacaaaagaa cccacactga ttatgacacg catactcgga gctatgctaa 34980
ccagcgtagc cccgatgtaa gcttgttgca tgggcggcga tataaaatgc aaggtgctgc 35040
tcaaaaaatc aggcaaagcc tcgcgcaaaa aagaaagcac atcgtagtca tgctcatgca 35100
gataaaggca ggtaagctcc ggaaccacca cagaaaaaga caccattttt ctctcaaaca 35160
ttgggccagg attctcctcg acgtcaccag cctgcttcag caggctgaag ttagtagctc 35220
cgcttccctt gtacagctcg tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt 35280
actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccaacttg atgttgacgt 35340
tgtaggcgcc gggcagctgc acgggcttct tggccttgta ggtggtcttg acctcagcgt 35400
cgtagtggcc gccgtccttc agcttcagcc tctgcttgat ctcgcccttc agggcgccgt 35460
cctcggggta catccgctcg gaggaggcct cccagcccat ggtcttcttc tgcattacgg 35520
ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct 35580
gcagggagga gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct 35640
cccacttgaa gccctcgggg aaggacagct tcaagtagtc ggggatgtcg gcggggtgct 35700
tcacgtaggc cttggagccg tacatgaact gaggggacag gatgtcccag gcgaagggca 35760
gggggccacc cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct 35820
cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga 35880
agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc accatgtctg 35940
cgggtttctg cataaacaca aaataaaata acaaaaaaac atttaaacat tagaagcctg 36000
tcttacaaca ggaaaaacaa cccttataag cataagacgg actacggcca tgccggcgtg 36060
accgtaaaaa aactggtcac cgtgattaaa aagcaccacc gacagctcct cggtcatgtc 36120
cggagtcata atgtaagact cggtaaacac atcaggttga ttcacatcgg tcagtgctaa 36180
aaagcgaccg aaatagcccg ggggaataca tacccgcagg cgtagagaca acattacagc 36240
ccccatagga ggtataacaa aattaatagg agagaaaaac acataaacac ctgaaaaacc 36300
ctcctgccta ggcaaaatag caccctcccg ctccagaaca acatacagcg cttccacagc 36360
ggcagccata acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa aacaccactc 36420
gacacggcac cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag agcgagtata 36480
tataggacta aaaaatgacg taacggttaa agtccacaaa aaacacccag aaaaccgcac 36540
gcgaacctac gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa tcgtcacttc 36600
cgttttccca cgttacgtca cttcccattt taagaaaact acaattccca acacatacaa 36660
gttactccgc cctaaaacct acgtcacccg ccccgttccc acgccccgcg ccacgtcaca 36720
aactccaccc cctcattatc atattggctt caatccaaaa taaggtatat tattgatgat 36780
g 36781
<210> 12
<211> 36899
<212> DNA
<213> 人工序列
<220>
<223> 合成腺病毒基因组YPet-P2A-纤维蛋白
<400> 12
attaccctgt tatccctaca tcatcaataa tataccttat tttggattga agccaatatg 60
ataatgaggg ggtggagttt gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta 120
gtagtgtggc ggaagtgtga tgttgcaagt gtggcggaac acatgtaagc gacggatgtg 180
gcaaaagtga cgtttttggt gtgcgccggt gtacacagga agtgacaatt ttcgcgcggt 240
tttaggcgga tgttgtagta aatttgggcg taaccgagta agatttggcc attttcgcgg 300
gaaaactgaa taagaggaag tgaaatctga ataattttgt gttactcata gcgcgtaata 360
tttgtctagg gccgcgggga ctttgaccgt ttacgtggag actcgcccag gtgtttttct 420
caggtgtttt ccgcgttccg ggtcaaagtt ggcgttttat tattatagtc agctgacgtg 480
tagtgtattt atacccggtg agttcctcaa gaggccactc ttgagtgcca gcgagtagag 540
ttttctcctc cgagccgctc cgacaccggg actgaaaatg agacatatta tctgccacgg 600
aggtgttatt accgaagaaa tggccgccag tcttttggac cagctgatcg aagaggtact 660
ggctgataat cttccacctc ctagccattt tgaaccacct acccttcacg aactgtatga 720
tttagacgtg acggcccccg aagatcccaa cgaggaggcg gtttcgcaga tttttcccga 780
ctctgtaatg ttggcggtgc aggaagggat tgacttactc acttttccgc cggcgcccgg 840
ttctccggag ccgcctcacc tttcccggca gcccgagcag ccggagcaga gagccttggg 900
tccggtttct atgccaaacc ttgtaccgga ggtgatcgat cttacctgcc acgaggctgg 960
ctttccaccc agtgacgacg aggatgaaga gggtgaggag tttgtgttag attatgtgga 1020
gcaccccggg cacggttgca ggtcttgtca ttatcaccgg aggaatacgg gggacccaga 1080
tattatgtgt tcgctttgct atatgaggac ctgtggcatg tttgtctaca gtaagtgaaa 1140
attatgggca gtgggtgata gagtggtggg tttggtgtgg taattttttt tttaattttt 1200
acagttttgt ggtttaaaga attttgtatt gtgatttttt taaaaggtcc tgtgtctgaa 1260
cctgagcctg agcccgagcc agaaccggag cctgcaagac ctacccgccg tcctaaaatg 1320
gcgcctgcta tcctgagacg cccgacatca cctgtgtcta gagaatgcaa tagtagtacg 1380
gatagctgtg actccggtcc ttctaacaca cctcctgaga tacacccggt ggtcccgctg 1440
tgccccatta aaccagttgc cgtgagagtt ggtgggcgtc gccaggctgt ggaatgtatc 1500
gaggacttgc ttaacgagcc tgggcaacct ttggacttga gctgtaaacg ccccaggcca 1560
taaggtgtaa acctgtgatt gcgtgtgtgg ttaacgcctt tgtttgctga atgagttgat 1620
gtaagtttaa taaagggtga gataatgttt aacttgcatg gcgtgttaaa tggggcgggg 1680
cttaaagggt atataatgcg ccgtgggcta atcttggtta catctgacct catggaggct 1740
tgggagtgtt tggaagattt ttctgctgtg cgtaacttgc tggaacagag ctctaacagt 1800
acctcttggt tttggaggtt tctgtggggc tcatcccagg caaagttagt ctgcagaatt 1860
aaggaggatt acaagtggga atttgaagag cttttgaaat cctgtggtga gctgtttgat 1920
tctttgaatc tgggtcacca ggcgcttttc caagagaagg tcatcaagac tttggatttt 1980
tccacaccgg ggcgcgctgc ggctgctgtt gcttttttga gttttataaa ggataaatgg 2040
agcgaagaaa cccatctgag cggggggtac ctgctggatt ttctggccat gcatctgtgg 2100
agagcggttg tgagacacaa gaatcgcctg ctactgttgt cttccgtccg cccggcgata 2160
ataccgacgg aggagcagca gcagcagcag gaggaagcca ggcggcggcg gcaggagcag 2220
agcccatgga acccgagagc cggcctggac cctcgggaat gaatgttgta caggtggctg 2280
aactgtatcc agaactgaga cgcattttga caattacaga ggatgggcag gggctaaagg 2340
gggtaaagag ggagcggggg gcttgtgagg ctacagagga ggctaggaat ctagctttta 2400
gcttaatgac cagacaccgt cctgagtgta ttacttttca acagatcaag gataattgcg 2460
ctaatgagct tgatctgctg gcgcagaagt attccataga gcagctgacc acttactggc 2520
tgcagccagg ggatgatttt gaggaggcta ttagggtata tgcaaaggtg gcacttaggc 2580
cagattgcaa gtacaagatc agcaaacttg taaatatcag gaattgttgc tacatttctg 2640
ggaacggggc cgaggtggag atagatacgg aggatagggt ggcctttaga tgtagcatga 2700
taaatatgtg gccgggggtg cttggcatgg acggggtggt tattatgaat gtaaggttta 2760
ctggccccaa ttttagcggt acggttttcc tggccaatac caaccttatc ctacacggtg 2820
taagcttcta tgggtttaac aatacctgtg tggaagcctg gaccgatgta agggttcggg 2880
gctgtgcctt ttactgctgc tggaaggggg tggtgtgtcg ccccaaaagc agggcttcaa 2940
ttaagaaatg cctctttgaa aggtgtacct tgggtatcct gtctgagggt aactccaggg 3000
tgcgccacaa tgtggcctcc gactgtggtt gcttcatgct agtgaaaagc gtggctgtga 3060
ttaagcataa catggtatgt ggcaactgcg aggacagggc ctctcagatg ctgacctgct 3120
cggacggcaa ctgtcacctg ctgaagacca ttcacgtagc cagccactct cgcaaggcct 3180
ggccagtgtt tgagcataac atactgaccc gctgttcctt gcatttgggt aacaggaggg 3240
gggtgttcct accttaccaa tgcaatttga gtcacactaa gatattgctt gagcccgaga 3300
gcatgtccaa ggtgaacctg aacggggtgt ttgacatgac catgaagatc tggaaggtgc 3360
tgaggtacga tgagacccgc accaggtgca gaccctgcga gtgtggcggt aaacatatta 3420
ggaaccagcc tgtgatgctg gatgtgaccg aggagctgag gcccgatcac ttggtgctgg 3480
cctgcacccg cgctgagttt ggctctagcg atgaagatac agattgaggt actgaaatgt 3540
gtgggcgtgg cttaagggtg ggaaagaata tataaggtgg gggtcttatg tagttttgta 3600
tctgttttgc agcagccgcc gccgcccaac ttttctatac aaagttgtaa tgagcaccaa 3660
ctcgtttgat ggaagcattg tgagctcata tttgacaacg cgcatgcccc catgggccgg 3720
ggtgcgtcag aatgtgatgg gctccagcat tgatggtcgc cccgtcctgc ccgcaaactc 3780
tactaccttg acctacgaga ccgtgtctgg aacgccgttg gagactgcag cctccgccgc 3840
cgcttcagcc gctgcagcca ccgcccgcgg gattgtgact gactttgctt tcctgagccc 3900
gcttgcaagc agtgcagctt cccgttcatc cgcccgcgat gacaagttga cggctctttt 3960
ggcacaattg gattctttga cccgggaact taatgtcgtt tctcagcagc tgttggatct 4020
gcgccagcag gtttctgccc tgaaggcttc ctcccctccc aatgcggttt aaaacataaa 4080
taaaaaacca gactctgttt ggatttggat caagcataag tgtcttgctg tctttattta 4140
ggggttttgc gcgcgcggta ggcccgggac cagcggtctc ggtcgttgag ggtcctgtgt 4200
attttttcca ggacgtggta aaggtgactc tggatgttca gatacatggg cataagcccg 4260
tctctggggt ggaggtagca ccactgcaga gcttcatgct gcggggtggt gttgtagatg 4320
atccagtcgt agcaggagcg ctgggcgtgg tgcctaaaaa tgtctttcag tagcaagctg 4380
attgccaggg gcaggccctt ggtgtaagtg tttacaaagc ggttaagctg ggatgggtgc 4440
atacgtgggg atatgagatg catcttggac tgtattttta ggttggctat gttcccagcc 4500
atatccctcc ggggattcat gttgtgcaga accaccagca cagtgtatcc ggtgcacttg 4560
ggaaatttgt catgtagctt agaaggaaat gcgtggaaga acttggagac gcccttgtga 4620
cctccaagat tttccatgca ttcgtccata atgatggcaa tgggcccacg ggcggcggcc 4680
tgggcgaaga tatttctggg atcactaacg tcatagttgt gttccaggat gagatcgtca 4740
taggccattt ttacaaagcg cgggcggagg gtgccagact gcggtataat ggttccatcc 4800
ggcccagggg cgtagttacc ctcacagatt tgcatttccc acgctttgag ttcagatggg 4860
gggatcatgt ctacctgcgg ggcgatgaag aaaacggttt ccggggtagg ggagatcagc 4920
tgggaagaaa gcaggttcct gagcagctgc gacttaccgc agccggtggg cccgtaaatc 4980
acacctatta ccgggtgcaa ctggtagtta agagagctgc agctgccgtc atccctgagc 5040
aggggggcca cttcgttaag catgtccctg actcgcatgt tttccctgac caaatccgcc 5100
agaaggcgct cgccgcccag cgatagcagt tcttgcaagg aagcaaagtt tttcaacggt 5160
ttgagaccgt ccgccgtagg catgcttttg agcgtttgac caagcagttc caggcggtcc 5220
cacagctcgg tcacctgctc tacggcatct cgatccagca tatctcctcg tttcgcgggt 5280
tggggcggct ttcgctgtac ggcagtagtc ggtgctcgtc cagacgggcc agggtcatgt 5340
ctttccacgg gcgcagggtc ctcgtcagcg tagtctgggt cacggtgaag gggtgcgctc 5400
cgggctgcgc gctggccagg gtgcgcttga ggctggtcct gctggtgctg aagcgctgcc 5460
ggtcttcgcc ctgcgcgtcg gccaggtagc atttgaccat ggtgtcatag tccagcccct 5520
ccgcggcgtg gcccttggcg cgcagcttgc ccttggagga ggcgccgcac gaggggcagt 5580
gcagactttt gagggcgtag agcttgggcg cgagaaatac cgattccggg gagtaggcat 5640
ccgcgccgca ggccccgcag acggtctcgc attccacgag ccaggtgagc tctggccgtt 5700
cggggtcaaa aaccaggttt cccccatgct ttttgatgcg tttcttacct ctggtttcca 5760
tgagccggtg tccacgctcg gtgacgaaaa ggctgtccgt gtccccgtat acagacttga 5820
gaggcctgtc ctcgagcggt gttccgcggt cctcctcgta tagaaactcg gaccactctg 5880
agacaaaggc tcgcgtccag gccagcacga aggaggctaa gtgggagggg tagcggtcgt 5940
tgtccactag ggggtccact cgctccaggg tgtgaagaca catgtcgccc tcttcggcat 6000
caaggaaggt gattggtttg taggtgtagg ccacgtgacc gggtgttcct gaaggggggc 6060
tataaaaggg ggtgggggcg cgttcgtcct cactctcttc cgcatcgctg tctgcgaggg 6120
ccagctgttg gggtgagtac tccctctgaa aagcgggcat gacttctgcg ctaagattgt 6180
cagtttccaa aaacgaggag gatttgatat tcacctggcc cgcggtgatg cctttgaggg 6240
tggccgcatc catctggtca gaaaagacaa tctttttgtt gtcaagcttg gtggcaaacg 6300
acccgtagag ggcgttggac agcaacttgg cgatggagcg cagggtttgg tttttgtcgc 6360
gatcggcgcg ctccttggcc gcgatgttta gctgcacgta ttcgcgcgca acgcaccgcc 6420
attcgggaaa gacggtggtg cgctcgtcgg gcaccaggtg cacgcgccaa ccgcggttgt 6480
gcagggtgac aaggtcaacg ctggtggcta cctctccgcg taggcgctcg ttggtccagc 6540
agaggcggcc gcccttgcgc gagcagaatg gcggtagggg gtctagctgc gtctcgtccg 6600
gggggtctgc gtccacggta aagaccccgg gcagcaggcg cgcgtcgaag tagtctatct 6660
tgcatccttg caagtctagc gcctgctgcc atgcgcgggc ggcaagcgcg cgctcgtatg 6720
ggttgagtgg gggaccccat ggcatggggt gggtgagcgc ggaggcgtac atgccgcaaa 6780
tgtcgtaaac gtagaggggc tctctgagta ttccaagata tgtagggtag catcttccac 6840
cgcggatgct ggcgcgcacg taatcgtata gttcgtgcga gggagcgagg aggtcgggac 6900
cgaggttgct acgggcgggc tgctctgctc ggaagactat ctgcctgaag atggcatgtg 6960
agttggatga tatggttgga cgctggaaga cgttgaagct ggcgtctgtg agacctaccg 7020
cgtcacgcac gaaggaggcg taggagtcgc gcagcttgtt gaccagctcg gcggtgacct 7080
gcacgtctag ggcgcagtag tccagggttt ccttgatgat gtcatactta tcctgtccct 7140
tttttttcca cagctcgcgg ttgaggacaa actcttcgcg gtctttccag tactcttgga 7200
tcggaaaccc gtcggcctcc gaacggtaag agcctagcat gtagaactgg ttgacggcct 7260
ggtaggcgca gcatcccttt tctacgggta gcgcgtatgc ctgcgcggcc ttccggagcg 7320
aggtgtgggt gagcgcaaag gtgtccctga ccatgacttt gaggtactgg tatttgaagt 7380
cagtgtcgtc gcatccgccc tgctcccaga gcaaaaagtc cgtgcgcttt ttggaacgcg 7440
gatttggcag ggcgaaggtg acatcgttga agagtatctt tcccgcgcga ggcataaagt 7500
tgcgtgtgat gcggaagggt cccggcacct cggaacggtt gttaattacc tgggcggcga 7560
gcacgatctc gtcaaagccg ttgatgttgt ggcccacaat gtaaagttcc aagaagcgcg 7620
ggatgccctt gatggaaggc aattttttaa gttcctcgta ggtgagctct tcaggggagc 7680
tgagcccgtg ctctgaaagg gcccagtctg caagatgagg gttggaagcg acgaatgagc 7740
tccacaggtc acgggccatt agcatttgca ggtggtcgcg aaaggtccta aactggcgac 7800
ctatggccat tttttctggg gtgatgcagt agaaggtaag cgggtcttgt tcccagcggt 7860
cccatccaag gttcgcggct aggtctcgcg cggcagtcac tagaggctca tctccgccga 7920
acttcatgac cagcatgaag ggcacgagct gcttcccaaa ggcccccatc caagtatagg 7980
tctctacatc gtaggtgaca aagagacgct cggtgcgagg atgcgagccg atcgggaaga 8040
actggatctc ccgccaccaa ttggaggagt ggctattgat gtggtgaaag tagaagtccc 8100
tgcgacgggc cgaacactcg tgctggcttt tgtaaaaacg tgcgcagtac tggcagcggt 8160
gcacgggctg tacatcctgc acgaggttga cctgacgacc gcgcacaagg aagcagagtg 8220
ggaatttgag cccctcgcct ggcgggtttg gctggtggtc ttctacttcg gctgcttgtc 8280
cttgaccgtc tggctgctcg aggggagtta cggtggatcg gaccaccacg ccgcgcgagc 8340
ccaaagtcca gatgtccgcg cgcggcggtc ggagcttgat gacaacatcg cgcagatggg 8400
agctgtccat ggtctggagc tcccgcggcg tcaggtcagg cgggagctcc tgcaggttta 8460
cctcgcatag acgggtcagg gcgcgggcta gatccaggtg atacctaatt tccaggggct 8520
ggttggtggc ggcgtcgatg gcttgcaaga ggccgcatcc ccgcggcgcg actacggtac 8580
cgcgcggcgg gcggtgggcc gcgggggtgt ccttggatga tgcatctaaa agcggtgacg 8640
cgggcgagcc cccggaggta gggggggctc cggacccgcc gggagagggg gcaggggcac 8700
gtcggcgccg cgcgcgggca ggagctggtg ctgcgcgcgt aggttgctgg cgaacgcgac 8760
gacgcggcgg ttgatctcct gaatctggcg cctctgcgtg aagacgacgg gcccggtgag 8820
cttgagcctg aaagagagtt cgacagaatc aatttcggtg tcgttgacgg cggcctggcg 8880
caaaatctcc tgcacgtctc ctgagttgtc ttgataggcg atctcggcca tgaactgctc 8940
gatctcttcc tcctggagat ctccgcgtcc ggctcgctcc acggtggcgg cgaggtcgtt 9000
ggaaatgcgg gccatgagct gcgagaaggc gttgaggcct ccctcgttcc agacgcggct 9060
gtagaccacg cccccttcgg catcgcgggc gcgcatgacc acctgcgcga gattgagctc 9120
cacgtgccgg gcgaagacgg cgtagtttcg caggcgctga aagaggtagt tgagggtggt 9180
ggcggtgtgt tctgccacga agaagtacat aacccagcgt cgcaacgtgg attcgttgat 9240
atcccccaag gcctcaaggc gctccatggc ctcgtagaag tccacggcga agttgaaaaa 9300
ctgggagttg cgcgccgaca cggttaactc ctcctccaga agacggatga gctcggcgac 9360
agtgtcgcgc acctcgcgct caaaggctac aggggcctct tcttcttctt caatctcctc 9420
ttccataagg gcctcccctt cttcttcttc tggcggcggt gggggagggg ggacacggcg 9480
gcgacgacgg cgcaccggga ggcggtcgac aaagcgctcg atcatctccc cgcggcgacg 9540
gcgcatggtc tcggtgacgg cgcggccgtt ctcgcggggg cgcagttgga agacgccgcc 9600
cgtcatgtcc cggttatggg ttggcggggg gctgccatgc ggcagggata cggcgctaac 9660
gatgcatctc aacaattgtt gtgtaggtac tccgccgccg agggacctga gcgagtccgc 9720
atcgaccgga tcggaaaacc tctcgagaaa ggcgtctaac cagtcacagt cgcaaggtag 9780
gctgagcacc gtggcgggcg gcagcgggcg gcggtcgggg ttgtttctgg cggaggtgct 9840
gctgatgatg taattaaagt aggcggtctt gagacggcgg atggtcgaca gaagcaccat 9900
gtccttgggt ccggcctgct gaatgcgcag gcggtcggcc atgccccagg cttcgttttg 9960
acatcggcgc aggtctttgt agtagtcttg catgagcctt tctaccggca cttcttcttc 10020
tccttcctct tgtcctgcat ctcttgcatc tatcgctgcg gcggcggcgg agtttggccg 10080
taggtggcgc cctcttcctc ccatgcgtgt gaccccgaag cccctcatcg gctgaagcag 10140
ggctaggtcg gcgacaacgc gctcggctaa tatggcctgc tgcacctgcg tgagggtaga 10200
ctggaagtca tccatgtcca caaagcggtg gtatgcgccc gtgttgatgg tgtaagtgca 10260
gttggccata acggaccagt taacggtctg gtgacccggc tgcgagagct cggtgtacct 10320
gagacgcgag taagccctcg agtcaaatac gtagtcgttg caagtccgca ccaggtactg 10380
gtatcccacc aaaaagtgcg gcggcggctg gcggtagagg ggccagcgta gggtggccgg 10440
ggctccgggg gcgagatctt ccaacataag gcgatgatat ccgtagatgt acctggacat 10500
ccaggtgatg ccggcggcgg tggtggaggc gcgcggaaag tcgcggacgc ggttccagat 10560
gttgcgcagc ggcaaaaagt gctccatggt cgggacgctc tggccggtca ggcgcgcgca 10620
atcgttgacg ctctagaccg tgcaaaagga gagcctgtaa gcgggcactc ttccgtggtc 10680
tggtggataa attcgcaagg gtatcatggc ggacgaccgg ggttcgagcc ccgtatccgg 10740
ccgtccgccg tgatccatgc ggttaccgcc cgcgtgtcga acccaggtgt gcgacgtcag 10800
acaacggggg agtgctcctt ttggcttcct tccaggcgcg gcggctgctg cgctagcttt 10860
tttggccact ggccgcgcgc agcgtaagcg gttaggctgg aaagcgaaag cattaagtgg 10920
ctcgctccct gtagccggag ggttattttc caagggttga gtcgcgggac ccccggttcg 10980
agtctcggac cggccggact gcggcgaacg ggggtttgcc tccccgtcat gcaagacccc 11040
gcttgcaaat tcctccggaa acagggacga gccccttttt tgcttttccc agatgcatcc 11100
ggtgctgcgg cagatgcgcc cccctcctca gcagcggcaa gagcaagagc agcggcagac 11160
atgcagggca ccctcccctc ctcctaccgc gtcaggaggg gcgacatccg cggttgacgc 11220
ggcagcagat ggtgattacg aacccccgcg gcgccgggcc cggcactacc tggacttgga 11280
ggagggcgag ggcctggcgc ggctaggagc gccctctcct gagcggtacc caagggtgca 11340
gctgaagcgt gatacgcgtg aggcgtacgt gccgcggcag aacctgtttc gcgaccgcga 11400
gggagaggag cccgaggaga tgcgggatcg aaagttccac gcagggcgcg agctgcggca 11460
tggcctgaat cgcgagcggt tgctgcgcga ggaggacttt gagcccgacg cgcgaaccgg 11520
gattagtccc gcgcgcgcac acgtggcggc cgccgacctg gtaaccgcat acgagcagac 11580
ggtgaaccag gagattaact ttcaaaaaag ctttaacaac cacgtgcgta cgcttgtggc 11640
gcgcgaggag gtggctatag gactgatgca tctgtgggac tttgtaagcg cgctggagca 11700
aaacccaaat agcaagccgc tcatggcgca gctgttcctt atagtgcagc acagcaggga 11760
caacgaggca ttcagggatg cgctgctaaa catagtagag cccgagggcc gctggctgct 11820
cgatttgata aacatcctgc agagcatagt ggtgcaggag cgcagcttga gcctggctga 11880
caaggtggcc gccatcaact attccatgct tagcctgggc aagttttacg cccgcaagat 11940
ataccatacc ccttacgttc ccatagacaa ggaggtaaag atcgaggggt tctacatgcg 12000
catggcgctg aaggtgctta ccttgagcga cgacctgggc gtttatcgca acgagcgcat 12060
ccacaaggcc gtgagcgtga gccggcggcg cgagctcagc gaccgcgagc tgatgcacag 12120
cctgcaaagg gccctggctg gcacgggcag cggcgataga gaggccgagt cctactttga 12180
cgcgggcgct gacctgcgct gggccccaag ccgacgcgcc ctggaggcag ctggggccgg 12240
acctgggctg gcggtggcac ccgcgcgcgc tggcaacgtc ggcggcgtgg aggaatatga 12300
cgaggacgat gagtacgagc cagaggacgg cgagtactaa gcggtgatgt ttctgatcag 12360
atgatgcaag acgcaacgga cccggcggtg cgggcggcgc tgcagagcca gccgtccggc 12420
cttaactcca cggacgactg gcgccaggtc atggaccgca tcatgtcgct gactgcgcgc 12480
aatcctgacg cgttccggca gcagccgcag gccaaccggc tctccgcaat tctggaagcg 12540
gtggtcccgg cgcgcgcaaa ccccacgcac gagaaggtgc tggcgatcgt aaacgcgctg 12600
gccgaaaaca gggccatccg gcccgacgag gccggcctgg tctacgacgc gctgcttcag 12660
cgcgtggctc gttacaacag cggcaacgtg cagaccaacc tggaccggct ggtgggggat 12720
gtgcgcgagg ccgtggcgca gcgtgagcgc gcgcagcagc agggcaacct gggctccatg 12780
gttgcactaa acgccttcct gagtacacag cccgccaacg tgccgcgggg acaggaggac 12840
tacaccaact ttgtgagcgc actgcggcta atggtgactg agacaccgca aagtgaggtg 12900
taccagtctg ggccagacta ttttttccag accagtagac aaggcctgca gaccgtaaac 12960
ctgagccagg ctttcaaaaa cttgcagggg ctgtgggggg tgcgggctcc cacaggcgac 13020
cgcgcgaccg tgtctagctt gctgacgccc aactcgcgcc tgttgctgct gctaatagcg 13080
cccttcacgg acagtggcag cgtgtcccgg gacacatacc taggtcactt gctgacactg 13140
taccgcgagg ccataggtca ggcgcatgtg gacgagcata ctttccagga gattacaagt 13200
gtcagccgcg cgctggggca ggaggacacg ggcagcctgg aggcaaccct aaactacctg 13260
ctgaccaacc ggcggcagaa gatcccctcg ttgcacagtt taaacagcga ggaggagcgc 13320
attttgcgct acgtgcagca gagcgtgagc cttaacctga tgcgcgacgg ggtaacgccc 13380
agcgtggcgc tggacatgac cgcgcgcaac atggaaccgg gcatgtatgc ctcaaaccgg 13440
ccgtttatca accgcctaat ggactacttg catcgcgcgg ccgccgtgaa ccccgagtat 13500
ttcaccaatg ccatcttgaa cccgcactgg ctaccgcccc ctggtttcta caccggggga 13560
ttcgaggtgc ccgagggtaa cgatggattc ctctgggacg acatagacga cagcgtgttt 13620
tccccgcaac cgcagaccct gctagagttg caacagcgcg agcaggcaga ggcggcgctg 13680
cgaaaggaaa gcttccgcag gccaagcagc ttgtccgatc taggcgctgc ggccccgcgg 13740
tcagatgcta gtagcccatt tccaagcttg atagggtctc ttaccagcac tcgcaccacc 13800
cgcccgcgcc tgctgggcga ggaggagtac ctaaacaact cgctgctgca gccgcagcgc 13860
gaaaaaaacc tgcctccggc atttcccaac aacgggatag agagcctagt ggacaagatg 13920
agtagatgga agacgtacgc gcaggagcac agggacgtgc caggcccgcg cccgcccacc 13980
cgtcgtcaaa ggcacgaccg tcagcggggt ctggtgtggg aggacgatga ctcggcagac 14040
gacagcagcg tcctggattt gggagggagt ggcaacccgt ttgcgcacct tcgccccagg 14100
ctggggagaa tgttttaaaa aaaaaaaagc atgatgcaaa ataaaaaact caccaaggcc 14160
atggcaccga gcgttggttt tcttgtattc cccttagtat gcggcgcgcg gcgatgtatg 14220
aggaaggtcc tcctccctcc tacgagagtg tggtgagcgc ggcgccagtg gcggcggcgc 14280
tgggttctcc cttcgatgct cccctggacc cgccgtttgt gcctccgcgg tacctgcggc 14340
ctaccggggg gagaaacagc atccgttact ctgagttggc acccctattc gacaccaccc 14400
gtgtgtacct ggtggacaac aagtcaacgg atgtggcatc cctgaactac cagaacgacc 14460
acagcaactt tctgaccacg gtcattcaaa acaatgacta cagcccgggg gaggcaagca 14520
cacagaccat caatcttgac gaccggtcgc actggggcgg cgacctgaaa accatcctgc 14580
ataccaacat gccaaatgtg aacgagttca tgtttaccaa taagtttaag gcgcgggtga 14640
tggtgtcgcg cttgcctact aaggacaatc aggtggagct gaaatacgag tgggtggagt 14700
tcacgctgcc cgagggcaac tactccgaga ccatgaccat agaccttatg aacaacgcga 14760
tcgtggagca ctacttgaaa gtgggcagac agaacggggt tctggaaagc gacatcgggg 14820
taaagtttga cacccgcaac ttcagactgg ggtttgaccc cgtcactggt cttgtcatgc 14880
ctggggtata tacaaacgaa gccttccatc cagacatcat tttgctgcca ggatgcgggg 14940
tggacttcac ccacagccgc ctgagcaact tgttgggcat ccgcaagcgg caacccttcc 15000
aggagggctt taggatcacc tacgatgatc tggagggtgg taacattccc gcactgttgg 15060
atgtggacgc ctaccaggcg agcttgaaag atgacaccga acagggcggg ggtggcgcag 15120
gcggcagcaa cagcagtggc agcggcgcgg aagagaactc caacgcggca gccgcggcaa 15180
tgcagccggt ggaggacatg aacgatcatg ccattcgcgg cgacaccttt gccacacggg 15240
ctgaggagaa gcgcgctgag gccgaagcag cggccgaagc tgccgccccc gctgcgcaac 15300
ccgaggtcga gaagcctcag aagaaaccgg tgatcaaacc cctgacagag gacagcaaga 15360
aacgcagtta caacctaata agcaatgaca gcaccttcac ccagtaccgc agctggtacc 15420
ttgcatacaa ctacggcgac cctcagaccg gaatccgctc atggaccctg ctttgcactc 15480
ctgacgtaac ctgcggctcg gagcaggtct actggtcgtt gccagacatg atgcaagacc 15540
ccgtgacctt ccgctccacg cgccagatca gcaactttcc ggtggtgggc gccgagctgt 15600
tgcccgtgca ctccaagagc ttctacaacg accaggccgt ctactcccaa ctcatccgcc 15660
agtttacctc tctgacccac gtgttcaatc gctttcccga gaaccagatt ttggcgcgcc 15720
cgccagcccc caccatcacc accgtcagtg aaaacgttcc tgctctcaca gatcacggga 15780
cgctaccgct gcgcaacagc atcggaggag tccagcgagt gaccattact gacgccagac 15840
gccgcacctg cccctacgtt tacaaggccc tgggcatagt ctcgccgcgc gtcctatcga 15900
gccgcacttt ttgagcaagc atgtccatcc ttatatcgcc cagcaataac acaggctggg 15960
gcctgcgctt cccaagcaag atgtttggcg gggccaagaa gcgctccgac caacacccag 16020
tgcgcgtgcg cgggcactac cgcgcgccct ggggcgcgca caaacgcggc cgcactgggc 16080
gcaccaccgt cgatgacgcc atcgacgcgg tggtggagga ggcgcgcaac tacacgccca 16140
cgccgccacc agtgtccaca gtggacgcgg ccattcagac cgtggtgcgc ggagcccggc 16200
gctatgctaa aatgaagaga cggcggaggc gcgtagcacg tcgccaccgc cgccgacccg 16260
gcactgccgc ccaacgcgcg gcggcggccc tgcttaaccg cgcacgtcgc accggccgac 16320
gggcggccat gcgggccgct cgaaggctgg ccgcgggtat tgtcactgtg ccccccaggt 16380
ccaggcgacg agcggccgcc gcagcagccg cggccattag tgctatgact cagggtcgca 16440
ggggcaacgt gtattgggtg cgcgactcgg ttagcggcct gcgcgtgccc gtgcgcaccc 16500
gccccccgcg caactagatt gcaagaaaaa actacttaga ctcgtactgt tgtatgtatc 16560
cagcggcggc ggcgcgcaac gaagctatgt ccaagcgcaa aatcaaagaa gagatgctcc 16620
aggtcatcgc gccggagatc tatggccccc cgaagaagga agagcaggat tacaagcccc 16680
gaaagctaaa gcgggtcaaa aagaaaaaga aagatgatga tgatgaactt gacgacgagg 16740
tggaactgct gcacgctacc gcgcccaggc gacgggtaca gtggaaaggt cgacgcgtaa 16800
aacgtgtttt gcgacccggc accaccgtag tctttacgcc cggtgagcgc tccacccgca 16860
cctacaagcg cgtgtatgat gaggtgtacg gcgacgagga cctgcttgag caggccaacg 16920
agcgcctcgg ggagtttgcc tacggaaagc ggcataagga catgctggcg ttgccgctgg 16980
acgagggcaa cccaacacct agcctaaagc ccgtaacact gcagcaggtg ctgcccgcgc 17040
ttgcaccgtc cgaagaaaag cgcggcctaa agcgcgagtc tggtgacttg gcacccaccg 17100
tgcagctgat ggtacccaag cgccagcgac tggaagatgt cttggaaaaa atgaccgtgg 17160
aacctgggct ggagcccgag gtccgcgtgc ggccaatcaa gcaggtggcg ccgggactgg 17220
gcgtgcagac cgtggacgtt cagataccca ctaccagtag caccagtatt gccaccgcca 17280
cagagggcat ggagacacaa acgtccccgg ttgcctcagc ggtggcggat gccgcggtgc 17340
aggcggtcgc tgcggccgcg tccaagacct ctacggaggt gcaaacggac ccgtggatgt 17400
ttcgcgtttc agccccccgg cgcccgcgcg gttcgaggaa gtacggcgcc gccagcgcgc 17460
tactgcccga atatgcccta catccttcca ttgcgcctac ccccggctat cgtggctaca 17520
cctaccgccc cagaagacga gcaactaccc gacgccgaac caccactgga acccgccgcc 17580
gccgtcgccg tcgccagccc gtgctggccc cgatttccgt gcgcagggtg gctcgcgaag 17640
gaggcaggac cctggtgctg ccaacagcgc gctaccaccc cagcatcgtt taaaagccgg 17700
tctttgtggt tcttgcagat atggccctca cctgccgcct ccgtttcccg gtgccgggat 17760
tccgaggaag aatgcaccgt aggaggggca tggccggcca cggcctgacg ggcggcatgc 17820
gtcgtgcgca ccaccggcgg cggcgcgcgt cgcaccgtcg catgcgcggc ggtatcctgc 17880
ccctccttat tccactgatc gccgcggcga ttggcgccgt gcccggaatt gcatccgtgg 17940
ccttgcaggc gcagagacac tgattaaaaa caagttgcat gtggaaaaat caaaataaaa 18000
agtctggact ctcacgctcg cttggtcctg taactatttt gtagaatgga agacatcaac 18060
tttgcgtctc tggccccgcg acacggctcg cgcccgttca tgggaaactg gcaagatatc 18120
ggcaccagca atatgagcgg tggcgccttc agctggggct cgctgtggag cggcattaaa 18180
aatttcggtt ccaccgttaa gaactatggc agcaaggcct ggaacagcag cacaggccag 18240
atgctgaggg ataagttgaa agagcaaaat ttccaacaaa aggtggtaga tggcctggcc 18300
tctggcatta gcggggtggt ggacctggcc aaccaggcag tgcaaaataa gattaacagt 18360
aagcttgatc cccgccctcc cgtagaggag cctccaccgg ccgtggagac agtgtctcca 18420
gaggggcgtg gcgaaaagcg tccgcgcccc gacagggaag aaactctggt gacgcaaata 18480
gacgagcctc cctcgtacga ggaggcacta aagcaaggcc tgcccaccac ccgtcccatc 18540
gcgcccatgg ctaccggagt gctgggccag cacacacccg taacgctgga cctgcctccc 18600
cccgccgaca cccagcagaa acctgtgctg ccaggcccga ccgccgttgt tgtaacccgt 18660
cctagccgcg cgtccctgcg ccgcgccgcc agcggtccgc gatcgttgcg gcccgtagcc 18720
agtggcaact ggcaaagcac actgaacagc atcgtgggtc tgggggtgca atccctgaag 18780
cgccgacgat gcttctgaat agctaacgtg tcgtatgtgt gtcatgtatg cgtccatgtc 18840
gccgccagag gagctgctga gccgccgcgc gcccgctttc caagatggct accccttcga 18900
tgatgccgca gtggtcttac atgcacatct cgggccagga cgcctcggag tacctgagcc 18960
ccgggctggt gcagtttgcc cgcgccaccg agacgtactt cagcctgaat aacaagttta 19020
gaaaccccac ggtggcgcct acgcacgacg tgaccacaga ccggtcccag cgtttgacgc 19080
tgcggttcat ccctgtggac cgtgaggata ctgcgtactc gtacaaggcg cggttcaccc 19140
tagctgtggg tgataaccgt gtgctggaca tggcttccac gtactttgac atccgcggcg 19200
tgctggacag gggccctact tttaagccct actctggcac tgcctacaac gccctggctc 19260
ccaagggtgc cccaaatcct tgcgaatggg atgaagctgc tactgctctt gaaataaacc 19320
tagaagaaga ggacgatgac aacgaagacg aagtagacga gcaagctgag cagcaaaaaa 19380
ctcacgtatt tgggcaggcg ccttattctg gtataaatat tacaaaggag ggtattcaaa 19440
taggtgtcga aggtcaaaca cctaaatatg ccgataaaac atttcaacct gaacctcaaa 19500
taggagaatc tcagtggtac gaaactgaaa ttaatcatgc agctgggaga gtccttaaaa 19560
agactacccc aatgaaacca tgttacggtt catatgcaaa acccacaaat gaaaatggag 19620
ggcaaggcat tcttgtaaag caacaaaatg gaaagctaga aagtcaagtg gaaatgcaat 19680
ttttctcaac tactgaggcg accgcaggca atggtgataa cttgactcct aaagtggtat 19740
tgtacagtga agatgtagat atagaaaccc cagacactca tatttcttac atgcccacta 19800
ttaaggaagg taactcacga gaactaatgg gccaacaatc tatgcccaac aggcctaatt 19860
acattgcttt tagggacaat tttattggtc taatgtatta caacagcacg ggtaatatgg 19920
gtgttctggc gggccaagca tcgcagttga atgctgttgt agatttgcaa gacagaaaca 19980
cagagctttc ataccagctt ttgcttgatt ccattggtga tagaaccagg tacttttcta 20040
tgtggaatca ggctgttgac agctatgatc cagatgttag aattattgaa aatcatggaa 20100
ctgaagatga acttccaaat tactgctttc cactgggagg tgtgattaat acagagactc 20160
ttaccaaggt aaaacctaaa acaggtcagg aaaatggatg ggaaaaagat gctacagaat 20220
tttcagataa aaatgaaata agagttggaa ataattttgc catggaaatc aatctaaatg 20280
ccaacctgtg gagaaatttc ctgtactcca acatagcgct gtatttgccc gacaagctaa 20340
agtacagtcc ttccaacgta aaaatttctg ataacccaaa cacctacgac tacatgaaca 20400
agcgagtggt ggctcccggg ttagtggact gctacattaa ccttggagca cgctggtccc 20460
ttgactatat ggacaacgtc aacccattta accaccaccg caatgctggc ctgcgctacc 20520
gctcaatgtt gctgggcaat ggtcgctatg tgcccttcca catccaggtg cctcagaagt 20580
tctttgccat taaaaacctc cttctcctgc cgggctcata cacctacgag tggaacttca 20640
ggaaggatgt taacatggtt ctgcagagct ccctaggaaa tgacctaagg gttgacggag 20700
ccagcattaa gtttgatagc atttgccttt acgccacctt cttccccatg gcccacaaca 20760
ccgcctccac gcttgaggcc atgcttagaa acgacaccaa cgaccagtcc tttaacgact 20820
atctctccgc cgccaacatg ctctacccta tacccgccaa cgctaccaac gtgcccatat 20880
ccatcccctc ccgcaactgg gcggctttcc gcggctgggc cttcacgcgc cttaagacta 20940
aggaaacccc atcactgggc tcgggctacg acccttatta cacctactct ggctctatac 21000
cctacctaga tggaaccttt tacctcaacc acacctttaa gaaggtggcc attacctttg 21060
actcttctgt cagctggcct ggcaatgacc gcctgcttac ccccaacgag tttgaaatta 21120
agcgctcagt tgacggggag ggttacaacg ttgcccagtg taacatgacc aaagactggt 21180
tcctggtaca aatgctagct aactacaaca ttggctacca gggcttctat atcccagaga 21240
gctacaagga ccgcatgtac tccttcttta gaaacttcca gcccatgagc cgtcaggtgg 21300
tggatgatac taaatacaag gactaccaac aggtgggcat cctacaccaa cacaacaact 21360
ctggatttgt tggctacctt gcccccacca tgcgcgaagg acaggcctac cctgctaact 21420
tcccctatcc gcttataggc aagaccgcag ttgacagcat tacccagaaa aagtttcttt 21480
gcgatcgcac cctttggcgc atcccattct ccagtaactt tatgtccatg ggcgcactca 21540
cagacctggg ccaaaacctt ctctacgcca actccgccca cgcgctagac atgacttttg 21600
aggtggatcc catggacgag cccacccttc tttatgtttt gtttgaagtc tttgacgtgg 21660
tccgtgtgca ccggccgcac cgcggcgtca tcgaaaccgt gtacctgcgc acgcccttct 21720
cggccggcaa cgccacaaca taaagaagca agcaacatca acaacagctg ccgccatggg 21780
ctccagtgag caggaactga aagccattgt caaagatctt ggttgtgggc catatttttt 21840
gggcacctat gacaagcgct ttccaggctt tgtttctcca cacaagctcg cctgcgccat 21900
agtcaatacg gccggtcgcg agactggggg cgtacactgg atggcctttg cctggaaccc 21960
gcactcaaaa acatgctacc tctttgagcc ctttggcttt tctgaccagc gactcaagca 22020
ggtttaccag tttgagtacg agtcactcct gcgccgtagc gccattgctt cttcccccga 22080
ccgctgtata acgctggaaa agtccaccca aagcgtacag gggcccaact cggccgcctg 22140
tggactattc tgctgcatgt ttctccacgc ctttgccaac tggccccaaa ctcccatgga 22200
tcacaacccc accatgaacc ttattaccgg ggtacccaac tccatgctca acagtcccca 22260
ggtacagccc accctgcgtc gcaaccagga acagctctac agcttcctgg agcgccactc 22320
gccctacttc cgcagccaca gtgcgcagat taggagcgcc acttcttttt gtcacttgaa 22380
aaacatgtaa aaataatgta ctagagacac tttcaataaa ggcaaatgct tttatttgta 22440
cactctcggg tgattattta cccccaccct tgccgtctgc gccgtttaaa aatcaaaggg 22500
gttctgccgc gcatcgctat gcgccactgg cagggacacg ttgcgatact ggtgtttagt 22560
gctccactta aactcaggca caaccatccg cggcagctcg gtgaagtttt cactccacag 22620
gctgcgcacc atcaccaacg cgtttagcag gtcgggcgcc gatatcttga agtcgcagtt 22680
ggggcctccg ccctgcgcgc gcgagttgcg atacacaggg ttgcagcact ggaacactat 22740
cagcgccggg tggtgcacgc tggccagcac gctcttgtcg gagatcagat ccgcgtccag 22800
gtcctccgcg ttgctcaggg cgaacggagt caactttggt agctgccttc ccaaaaaggg 22860
cgcgtgccca ggctttgagt tgcactcgca ccgtagtggc atcaaaaggt gaccgtgccc 22920
ggtctgggcg ttaggataca gcgcctgcat aaaagccttg atctgcttaa aagccacctg 22980
agcctttgcg ccttcagaga agaacatgcc gcaagacttg ccggaaaact gattggccgg 23040
acaggccgcg tcgtgcacgc agcaccttgc gtcggtgttg gagatctgca ccacatttcg 23100
gccccaccgg ttcttcacga tcttggcctt gctagactgc tccttcagcg cgcgctgccc 23160
gttttcgctc gtcacatcca tttcaatcac gtgctcctta tttatcataa tgcttccgtg 23220
tagacactta agctcgcctt cgatctcagc gcagcggtgc agccacaacg cgcagcccgt 23280
gggctcgtga tgcttgtagg tcacctctgc aaacgactgc aggtacgcct gcaggaatcg 23340
ccccatcatc gtcacaaagg tcttgttgct ggtgaaggtc agctgcaacc cgcggtgctc 23400
ctcgttcagc caggtcttgc atacggccgc cagagcttcc acttggtcag gcagtagttt 23460
gaagttcgcc tttagatcgt tatccacgtg gtacttgtcc atcagcgcgc gcgcagcctc 23520
catgcccttc tcccacgcag acacgatcgg cacactcagc gggttcatca ccgtaatttc 23580
actttccgct tcgctgggct cttcctcttc ctcttgcgtc cgcataccac gcgccactgg 23640
gtcgtcttca ttcagccgcc gcactgtgcg cttacctcct ttgccatgct tgattagcac 23700
cggtgggttg ctgaaaccca ccatttgtag cgccacatct tctctttctt cctcgctgtc 23760
cacgattacc tctggtgatg gcgggcgctc gggcttggga gaagggcgct tctttttctt 23820
cttgggcgca atggccaaat ccgccgccga ggtcgatggc cgcgggctgg gtgtgcgcgg 23880
caccagcgcg tcttgtgatg agtcttcctc gtcctcggac tcgatacgcc gcctcatccg 23940
cttttttggg ggcgcccggg gaggcggcgg cgacggggac ggggacgaca cgtcctccat 24000
ggttggggga cgtcgcgccg caccgcgtcc gcgctcgggg gtggtttcgc gctgctcctc 24060
ttcccgactg gccatttcct tctcctatag gcagaaaaag atcatggagt cagtcgagaa 24120
gaaggacagc ctaaccgccc cctctgagtt cgccaccacc gcctccaccg atgccgccaa 24180
cgcgcctacc accttccccg tcgaggcacc cccgcttgag gaggaggaag tgattatcga 24240
gcaggaccca ggttttgtaa gcgaagacga cgaggaccgc tcagtaccaa cagaggataa 24300
aaagcaagac caggacaacg cagaggcaaa cgaggaacaa gtcgggcggg gggacgaaag 24360
gcatggcgac tacctagatg tgggagacga cgtgctgttg aagcatctgc agcgccagtg 24420
cgccattatc tgcgacgcgt tgcaagagcg cagcgatgtg cccctcgcca tagcggatgt 24480
cagccttgcc tacgaacgcc acctattctc accgcgcgta ccccccaaac gccaagaaaa 24540
cggcacatgc gagcccaacc cgcgcctcaa cttctacccc gtatttgccg tgccagaggt 24600
gcttgccacc tatcacatct ttttccaaaa ctgcaagata cccctatcct gccgtgccaa 24660
ccgcagccga gcggacaagc agctggcctt gcggcagggc gctgtcatac ctgatatcgc 24720
ctcgctcaac gaagtgccaa aaatctttga gggtcttgga cgcgacgaga agcgcgcggc 24780
aaacgctctg caacaggaaa acagcgaaaa tgaaagtcac tctggagtgt tggtggaact 24840
cgagggtgac aacgcgcgcc tagccgtact aaaacgcagc atcgaggtca cccactttgc 24900
ctacccggca cttaacctac cccccaaggt catgagcaca gtcatgagtg agctgatcgt 24960
gcgccgtgcg cagcccctgg agagggatgc aaatttgcaa gaacaaacag aggagggcct 25020
acccgcagtt ggcgacgagc agctagcgcg ctggcttcaa acgcgcgagc ctgccgactt 25080
ggaggagcga cgcaaactaa tgatggccgc agtgctcgtt accgtggagc ttgagtgcat 25140
gcagcggttc tttgctgacc cggagatgca gcgcaagcta gaggaaacat tgcactacac 25200
ctttcgacag ggctacgtac gccaggcctg caagatctcc aacgtggagc tctgcaacct 25260
ggtctcctac cttggaattt tgcacgaaaa ccgccttggg caaaacgtgc ttcattccac 25320
gctcaagggc gaggcgcgcc gcgactacgt ccgcgactgc gtttacttat ttctatgcta 25380
cacctggcag acggccatgg gcgtttggca gcagtgcttg gaggagtgca acctcaagga 25440
gctgcagaaa ctgctaaagc aaaacttgaa ggacctatgg acggccttca acgagcgctc 25500
cgtggccgcg cacctggcgg acatcatttt ccccgaacgc ctgcttaaaa ccctgcaaca 25560
gggtctgcca gacttcacca gtcaaagcat gttgcagaac tttaggaact ttatcctaga 25620
gcgctcagga atcttgcccg ccacctgctg tgcacttcct agcgactttg tgcccattaa 25680
gtaccgcgaa tgccctccgc cgctttgggg ccactgctac cttctgcagc tagccaacta 25740
ccttgcctac cactctgaca taatggaaga cgtgagcggt gacggtctac tggagtgtca 25800
ctgtcgctgc aacctatgca ccccgcaccg ctccctggtt tgcaattcgc agctgcttaa 25860
cgaaagtcaa attatcggta cctttgagct gcagggtccc tcgcctgacg aaaagtccgc 25920
ggctccgggg ttgaaactca ctccggggct gtggacgtcg gcttaccttc gcaaatttgt 25980
acctgaggac taccacgccc acgagattag gttctacgaa gaccaatccc gcccgccaaa 26040
tgcggagctt accgcctgcg tcattaccca gggccacatt cttggccaat tgcaagccat 26100
caacaaagcc cgccaagagt ttctgctacg aaagggacgg ggggtttact tggaccccca 26160
gtccggcgag gagctcaacc caatcccccc gccgccgcag ccctatcagc agcagccgcg 26220
ggcccttgct tcccaggatg gcacccaaaa agaagctgca gctgccgccg ccacccacgg 26280
acgaggagga atactgggac agtcaggcag aggaggtttt ggacgaggag gaggaggaca 26340
tgatggaaga ctgggagagc ctagacgagg aagcttccga ggtcgaagag gtgtcagacg 26400
aaacaccgtc accctcggtc gcattcccct cgccggcgcc ccagaaatcg gcaaccggtt 26460
ccagcatggc tacaacctcc gctcctcagg cgccgccggc actgcccgtt cgccgaccca 26520
accgtagatg ggacaccact ggaaccaggg ccggtaagtc caagcagccg ccgccgttag 26580
cccaagagca acaacagcgc caaggctacc gctcatggcg cgggcacaag aacgccatag 26640
ttgcttgctt gcaagactgt gggggcaaca tctccttcgc ccgccgcttt cttctctacc 26700
atcacggcgt ggccttcccc cgtaacatcc tgcattacta ccgtcatctc tacagcccat 26760
actgcaccgg cggcagcggc agcggcagca acagcagcgg ccacacagaa gcaaaggcga 26820
ccggatagca agactctgac aaagcccaag aaatccacag cggcggcagc agcaggagga 26880
ggagcgctgc gtctggcgcc caacgaaccc gtatcgaccc gcgagcttag aaacaggatt 26940
tttcccactc tgtatgctat atttcaacag agcaggggcc aagaacaaga gctgaaaata 27000
aaaaacaggt ctctgcgatc cctcacccgc agctgcctgt atcacaaaag cgaagatcag 27060
cttcggcgca cgctggaaga cgcggaggct ctcttcagta aatactgcgc gctgactctt 27120
aaggactagt ttcgcgccct ttctcaaatt taagcgcgaa aactacgtca tctccagcgg 27180
ccacacccgg cgccagcacc tgtcgtcagc gccatttcaa ctttgtatac aaaagttgtg 27240
atgagcaagg aaattcccac gccctacatg tggagttacc agccacaaat gggacttgcg 27300
gctggagctg cccaagacta ctcaacccga ataaactaca tgagcgcggg accccacatg 27360
atatcccggg tcaacggaat acgcgcccac cgaaaccgaa ttctcctgga acaggcggct 27420
attaccacca cacctcgtaa taaccttaat ccccgtagtt ggcccgctgc cctggtgtac 27480
caggaaagtc ccgctcccac cactgtggta cttcccagag acgcccaggc cgaagttcag 27540
atgactaact caggggcgca gcttgcgggc ggctttcgtc acagggtgcg gtcgcccggg 27600
cagggtataa ctcacctgac aatcagaggg cgaggtattc agctcaacga cgagtcggtg 27660
agctcctcgc ttggtctccg tccggacggg acatttcaga tcggcggcgc cggccgctct 27720
tcattcacgc ctcgtcaggc aatcctaact ctgcagacct cgtcctctga gccgcgctct 27780
ggaggcattg gaactctgca atttattgag gagtttgtgc catcggtcta ctttaacccc 27840
ttctcgggac ctcccggcca ctatccggat caatttattc ctaactttga cgcggtaaag 27900
gactcggcgg acggctacga ctgaatgtta agtggagagg cagagcaact gcgcctgaaa 27960
cacctggtcc actgtcgccg ccacaagtgc tttgcccgcg actccggtga gttttgctac 28020
tttgaattgc ccgaggatca tatcgagggc ccggcgcacg gcgtccggct taccgcccag 28080
ggagagcttg cccgtagcct gattcgggag tttacccagc gccccctgct agttgagcgg 28140
gacaggggac cctgtgttct cactgtgatt tgcaactgtc ctaaccctgg attacatcaa 28200
gatctttgtt gccatctctg tgctgagtat aataaataca gaaattaaaa tatactgggg 28260
ctcctatcgc catcctgtaa acgccaccgt cttcacccgc ccaagcaaac caaggcgaac 28320
cttacctggt acttttaaca tctctccctc tgtgatttac aacagtttca acccagacgg 28380
agtgagtcta cgagagaacc tctccgagct cagctactcc atcagaaaaa acaccaccct 28440
ccttacctgc cgggaacgta cgagtgcgtc accggccgct gcaccacacc taccgcctga 28500
ccgtaaacca gactttttcc ggacagacct caataactct gtttaccaga acaggaggtg 28560
agcttagaaa acccttaggg tattaggcca aaggcgcagc tactgtgggg tttatgaaca 28620
attcaagcaa ctctacgggc tattctaatt caggtttctc tagaatcggg gttggggtta 28680
ttctctgtct tgtgattctc tttattctta tactaacgct tctctgccta aggctcgccg 28740
cctgctgtgt gcacatttgc atttattgtc agctttttaa acgctggggt cgccacccaa 28800
gatgattagg tacataatcc taggtttact cacccttgcg tcagcccacg gtaccaccca 28860
aaaggtggat tttaaggagc cagcctgtaa tgttacattc gcagctgaag ctaatgagtg 28920
caccactctt ataaaatgca ccacagaaca tgaaaagctg cttattcgcc acaaaaacaa 28980
aattggcaag tatgctgttt atgctatttg gcagccaggt gacactacag agtataatgt 29040
tacagttttc cagggtaaaa gtcataaaac ttttatgtat acttttccat tttatgaaat 29100
gtgcgacatt accatgtaca tgagcaaaca gtataagttg tggcccccac aaaattgtgt 29160
ggaaaacact ggcactttct gctgcactgc tatgctaatt acagtgctcg ctttggtctg 29220
taccctactc tatattaaat acaaaagcag acgcagcttt attgaggaaa agaaaatgcc 29280
ttaatttact aagttacaaa gctaatgtca ccactaactg ctttactcgc tgcttgcaaa 29340
acaaattcaa aaagttagca ttataattag aataggattt aaaccccccg gtcatttcct 29400
gctcaatacc attcccctga acaattgact ctatgtggga tatgctccag cgctacaacc 29460
ttgaagtcag gcttcctgga tgtcagcatc tgactttggc cagcacctgt cccgcggatt 29520
tgttccagtc caactacagc gacccaccct aacagagatg accaacacaa ccaacgcggc 29580
cgccgctacc ggacttacat ctaccacaaa tacaccccaa gtttctgcct ttgtcaataa 29640
ctgggataac ttgggcatgt ggtggttctc catagcgctt atgtttgtat gccttattat 29700
tatgtggctc atctgctgcc taaagcgcaa acgcgcccga ccacccatct atagtcccat 29760
cattgtgcta cacccaaaca atgatggaat ccatagattg gacggactga aacacatgtt 29820
cttttctctt acagtatgat taaatgagac atgattcctc gagtttttat attactgacc 29880
cttgttgcgc ttttttgtgc gtgctccaca ttggctgcgg tttctcacat cgaagtagac 29940
tgcattccag ccttcacagt ctatttgctt tacggatttg tcaccctcac gctcatctgc 30000
agcctcatca ctgtggtcat cgcctttatc cagtgcattg actgggtctg tgtgcgcttt 30060
gcatatctca gacaccatcc ccagtacagg gacaggacta tagctgagct tcttagaatt 30120
ctttaattat gaaatttact gtgacttttc tgctgattat ttgcacccta tctgcgtttt 30180
gttccccgac ctccaagcct caaagacata tatcatgcag attcactcgt atatggaata 30240
ttccaagttg ctacaatgaa aaaagcgatc tttccgaagc ctggttatat gcaatcatct 30300
ctgttatggt gttctgcagt accatcttag ccctagctat atatccctac cttgacattg 30360
gctggaacgc aatagatgcc atgaaccacc caactttccc cgcgcccgct atgcttccac 30420
tgcaacaagt tgttgccggc ggctttgtcc cagccaatca gcctcgccca ccttctccca 30480
cccccactga aatcagctac tttaatctaa caggaggaga tgactgacac cctagatcta 30540
gaaatggacg gaattattac agagcagcgc ctgctagaaa gacgcagggc agcggccgag 30600
caacagcgca tgaatcaaga gctccaagac atggttaact tgcaccagtg caaaaggggt 30660
atcttttgtc tggtaaagca ggccaaagtc acctacgaca gtaataccac cggacaccgc 30720
cttagctaca agttgccaac caagcgtcag aaattggtgg tcatggtggg agaaaagccc 30780
attaccataa ctcagcactc ggtagaaacc gaaggctgca ttcactcacc ttgtcaagga 30840
cctgaggatc tctgcaccct tattaagacc ctgtgcggtc tcaaagatct tattcccttt 30900
aactaataaa aaaaaataat aaagcatcac ttacttaaaa tcagttagca aatttctgtc 30960
cagtttattc agcagcacct ccttgccctc ctcccagctc tggtattgca gcttcctcct 31020
ggctgcaaac tttctccaca atctaaatgg aatgtcagtt tcctcctgtt cctgtccatc 31080
cgcacccact atcttcatgt tgttgcagat ggtgagcaaa ggcgaagagc tgttcaccgg 31140
cgtggtgccc atcctggtgg agctggacgg cgacgtgaac ggccacaagt tcagcgtgag 31200
cggcgagggc gagggcgacg ccacctacgg caagctgacc ctgaagctgc tgtgcaccac 31260
cggcaagctg cccgtgccct ggcccaccct ggtgaccacc ctgggctacg gcgtgcagtg 31320
cttcgcccgg taccccgacc acatgaagca gcacgacttc ttcaagagcg ccatgcccga 31380
gggctacgtg caggagcgga ccatcttctt caaggacgac ggcaactaca agacccgggc 31440
cgaggtgaag ttcgagggcg acaccctggt gaaccggatc gagctgaagg gcatcgactt 31500
caaggaggac ggcaacatcc tgggccacaa gctggagtac aactacaaca gccacaacgt 31560
gtacatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccggcacaa 31620
catcgaggac ggcggcgtgc agctggccga ccactaccag cagaacaccc ccatcggcga 31680
cggccccgtg ctgctgcccg acaaccacta cctgagctac cagagcgccc tgttcaagga 31740
ccccaacgag aagcgggacc acatggtgct gctggagttc ctgaccgccg ccggcatcac 31800
cgagggcatg aacgagctct ataagggaag cggagctact aacttcagcc tgctgaagca 31860
ggctggtgac gtcgaggaga atcctggccc aatgaagcgc gcaagaccgt ctgaagatac 31920
cttcaacccc gtgtatccat atgacacgga aaccggtcct ccaactgtgc cttttcttac 31980
tcctcccttt gtatccccca atgggtttca agagagtccc cctggggtac tctctttgcg 32040
cctatccgaa cctctagtta cctccaatgg catgcttgcg ctcaaaatgg gcaacggcct 32100
ctctctggac gaggccggca accttacctc ccaaaatgta accactgtga gcccacctct 32160
caaaaaaacc aagtcaaaca taaacctgga aatatctgca cccctcacag ttacctcaga 32220
agccctaact gtggctgccg ccgcacctct aatggtcgcg ggcaacacac tcaccatgca 32280
atcacaggcc ccgctaaccg tgcacgactc caaacttagc attgccaccc aaggacccct 32340
cacagtgtca gaaggaaagc tagccctgca aacatcaggc cccctcacca ccaccgatag 32400
cagtaccctt actatcactg cctcaccccc tctaactact gccactggta gcttgggcat 32460
tgacttgaaa gagcccattt atacacaaaa tggaaaacta ggactaaagt acggggctcc 32520
tttgcatgta acagacgacc taaacacttt gaccgtagca actggtccag gtgtgactat 32580
taataatact tccttgcaaa ctaaagttac tggagccttg ggttttgatt cacaaggcaa 32640
tatgcaactt aatgtagcag gaggactaag gattgattct caaaacagac gccttatact 32700
tgatgttagt tatccgtttg atgctcaaaa ccaactaaat ctaagactag gacagggccc 32760
tctttttata aactcagccc acaacttgga tattaactac aacaaaggcc tttacttgtt 32820
tacagcttca aacaattcca aaaagcttga ggttaaccta agcactgcca aggggttgat 32880
gtttgacgct acagccatag ccattaatgc aggagatggg cttgaatttg gttcacctaa 32940
tgcaccaaac acaaatcccc tcaaaacaaa aattggccat ggcctagaat ttgattcaaa 33000
caaggctatg gttcctaaac taggaactgg ccttagtttt gacagcacag gtgccattac 33060
agtaggaaac aaaaataatg ataagctaac tttgtggacc acaccagctc catctcctaa 33120
ctgtagacta aatgcagaga aagatgctaa actcactttg gtcttaacaa aatgtggcag 33180
tcaaatactt gctacagttt cagttttggc tgttaaaggc agtttggctc caatatctgg 33240
aacagttcaa agtgctcatc ttattataag atttgacgaa aatggagtgc tactaaacaa 33300
ttccttcctg gacccagaat attggaactt tagaaatgga gatcttactg aaggcacagc 33360
ctatacaaac gctgttggat ttatgcctaa cctatcagct tatccaaaat ctcacggtaa 33420
aactgccaaa agtaacattg tcagtcaagt ttacttaaac ggagacaaaa ctaaacctgt 33480
aacactaacc attacactaa acggtacaca ggaaacagga gacacaactc caagtgcata 33540
ctctatgtca ttttcatggg actggtctgg ccacaactac attaatgaaa tatttgccac 33600
atcctcttac actttttcat acattgccca agaataaaga atcgtttgtg ttatgtttca 33660
acgtgtttat ttttcaattg cagaaaattt cgaatcattt ttcattcagt agtatagccc 33720
caccaccaca tagcttatac agatcaccgt acctctttat ttttcaattg cagaaaattt 33780
caagtcattt ttcattcagt agtatagccc caccaccaca tagcttatac agatcaccgt 33840
acctcaactt tgtataataa agttgtaatc aaactcacag aaccctagta ttcaacctgc 33900
cacctccctc ccaacacaca gagtacacag tcctttctcc ccggctggcc ttaaaaagca 33960
tcatatcatg ggtaacagac atattcttag gtgttatatt ccacacggtt tcctgtcgag 34020
ccaaacgctc atcagtgata ttaataaact ccccgggcag ctcacttaag ttcatgtcgc 34080
tgtccagctg ctgagccaca ggctgctgtc caacttgcgg ttgcttaacg ggcggcgaag 34140
gagaagtcca cgcctacatg ggggtagagt cataatcgtg catcaggata gggcggtggt 34200
gctgcagcag cgcgcgaata aactgctgcc gccgccgctc cgtcctgcag gaatacaaca 34260
tggcagtggt ctcctcagcg atgattcgca ccgcccgcag cataaggcgc cttgtcctcc 34320
gggcacagca gcgcaccctg atctcactta aatcagcaca gtaactgcag cacagcacca 34380
caatattgtt caaaatccca cagtgcaagg cgctgtatcc aaagctcatg gcggggacca 34440
cagaacccac gtggccatca taccacaagc gcaggtagat taagtggcga cccctcataa 34500
acacgctgga cataaacatt acctcttttg gcatgttgta attcaccacc tcccggtacc 34560
atataaacct ctgattaaac atggcgccat ccaccaccat cctaaaccag ctggccaaaa 34620
cctgcccgcc ggctatacac tgcagggaac cgggactgga acaatgacag tggagagccc 34680
aggactcgta accatggatc atcatgctcg tcatgatatc aatgttggca caacacaggc 34740
acacgtgcat acacttcctc aggattacaa gctcctcccg cgttagaacc atatcccagg 34800
gaacaaccca ttcctgaatc agcgtaaatc ccacactgca gggaagacct cgcacgtaac 34860
tcacgttgtg cattgtcaaa gtgttacatt cgggcagcag cggatgatcc tccagtatgg 34920
tagcgcgggt ttctgtctca aaaggaggta gacgatccct actgtacgga gtgcgccgag 34980
acaaccgaga tcgtgttggt cgtagtgtca tgccaaatgg aacgccggac gtagtcatat 35040
ttcctgaagc aaaaccaggt gcgggcgtga caaacagatc tgcgtctccg gtctcgccgc 35100
ttagatcgct ctgtgtagta gttgtagtat atccactctc tcaaagcatc caggcgcccc 35160
ctggcttcgg gttctatgta aactccttca tgcgccgctg ccctgataac atccaccacc 35220
gcagaataag ccacacccag ccaacctaca cattcgttct gcgagtcaca cacgggagga 35280
gcgggaagag ctggaagaac catgtttttt tttttattcc aaaagattat ccaaaacctc 35340
aaaatgaaga tctattaagt gaacgcgctc ccctccggtg gcgtggtcaa actctacagc 35400
caaagaacag ataatggcat ttgtaagatg ttgcacaatg gcttccaaaa ggcaaacggc 35460
cctcacgtcc aagtggacgt aaaggctaaa cccttcaggg tgaatctcct ctataaacat 35520
tccagcacct tcaaccatgc ccaaataatt ctcatctcgc caccttctca atatatctct 35580
aagcaaatcc cgaatattaa gtccggccat tgtaaaaatc tgctccagag cgccctccac 35640
cttcagcctc aagcagcgaa tcatgattgc aaaaattcag gttcctcaca gacctgtata 35700
agattcaaaa gcggaacatt aacaaaaata ccgcgatccc gtaggtccct tcgcagggcc 35760
agctgaacat aatcgtgcag gtctgcacgg accagcgcgg ccacttcccc gccaggaacc 35820
atgacaaaag aacccacact gattatgaca cgcatactcg gagctatgct aaccagcgta 35880
gccccgatgt aagcttgttg catgggcggc gatataaaat gcaaggtgct gctcaaaaaa 35940
tcaggcaaag cctcgcgcaa aaaagaaagc acatcgtagt catgctcatg cagataaagg 36000
caggtaagct ccggaaccac cacagaaaaa gacaccattt ttctctcaaa catgtctgcg 36060
ggtttctgca taaacacaaa ataaaataac aaaaaaacat ttaaacatta gaagcctgtc 36120
ttacaacagg aaaaacaacc cttataagca taagacggac tacggccatg ccggcgtgac 36180
cgtaaaaaaa ctggtcaccg tgattaaaaa gcaccaccga cagctcctcg gtcatgtccg 36240
gagtcataat gtaagactcg gtaaacacat caggttgatt cacatcggtc agtgctaaaa 36300
agcgaccgaa atagcccggg ggaatacata cccgcaggcg tagagacaac attacagccc 36360
ccataggagg tataacaaaa ttaataggag agaaaaacac ataaacacct gaaaaaccct 36420
cctgcctagg caaaatagca ccctcccgct ccagaacaac atacagcgct tccacagcgg 36480
cagccataac agtcagcctt accagtaaaa aagaaaacct attaaaaaaa caccactcga 36540
cacggcacca gctcaatcag tcacagtgta aaaaagggcc aagtgcagag cgagtatata 36600
taggactaaa aaatgacgta acggttaaag tccacaaaaa acacccagaa aaccgcacgc 36660
gaacctacgc ccagaaacga aagccaaaaa acccacaact tcctcaaatc gtcacttccg 36720
ttttcccacg ttacgtcact tcccatttta agaaaactac aattcccaac acatacaagt 36780
tactccgccc taaaacctac gtcacccgcc ccgttcccac gccccgcgcc acgtcacaaa 36840
ctccaccccc tcattatcat attggcttca atccaaaata aggtatatta ttgatgatg 36899
<210> 13
<211> 36794
<212> DNA
<213> 人工序列
<220>
<223> 合成腺病毒基因组纤维蛋白-P2A-YPet
<400> 13
catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60
ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120
gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180
gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240
taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300
agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360
gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420
cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480
tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540
tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600
aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660
tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720
cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780
gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840
cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900
ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960
cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020
caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080
ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140
tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200
gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260
ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320
cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380
ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440
gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500
cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560
ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620
gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680
cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740
ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800
tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860
gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920
caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980
gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040
agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100
aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160
cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220
gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280
gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340
gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400
gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460
tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520
ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580
tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640
agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700
tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760
gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820
acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880
gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940
aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000
ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060
gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120
tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180
acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240
aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300
tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360
gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420
tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480
ttggctctag cgatgaagat acagattgag gtactgaaat gtgtgggcgt ggcttaaggg 3540
tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg 3600
ccgccgccca acttttctat acaaagttgt aatgagcacc aactcgtttg atggaagcat 3660
tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat 3720
gggctccagc attgatggtc gccccgtcct gcccgcaaac tctactacct tgacctacga 3780
gaccgtgtct ggaacgccgt tggagactgc agcctccgcc gccgcttcag ccgctgcagc 3840
caccgcccgc gggattgtga ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc 3900
ttcccgttca tccgcccgcg atgacaagtt gacggctctt ttggcacaat tggattcttt 3960
gacccgggaa cttaatgtcg tttctcagca gctgttggat ctgcgccagc aggtttctgc 4020
cctgaaggct tcctcccctc ccaatgcggt ttaaaacata aataaaaaac cagactctgt 4080
ttggatttgg atcaagcata agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg 4140
taggcccggg accagcggtc tcggtcgttg agggtcctgt gtattttttc caggacgtgg 4200
taaaggtgac tctggatgtt cagatacatg ggcataagcc cgtctctggg gtggaggtag 4260
caccactgca gagcttcatg ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag 4320
cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc tgattgccag gggcaggccc 4380
ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt gcatacgtgg ggatatgaga 4440
tgcatcttgg actgtatttt taggttggct atgttcccag ccatatccct ccggggattc 4500
atgttgtgca gaaccaccag cacagtgtat ccggtgcact tgggaaattt gtcatgtagc 4560
ttagaaggaa atgcgtggaa gaacttggag acgcccttgt gacctccaag attttccatg 4620
cattcgtcca taatgatggc aatgggccca cgggcggcgg cctgggcgaa gatatttctg 4680
ggatcactaa cgtcatagtt gtgttccagg atgagatcgt cataggccat ttttacaaag 4740
cgcgggcgga gggtgccaga ctgcggtata atggttccat ccggcccagg ggcgtagtta 4800
ccctcacaga tttgcatttc ccacgctttg agttcagatg gggggatcat gtctacctgc 4860
ggggcgatga agaaaacggt ttccggggta ggggagatca gctgggaaga aagcaggttc 4920
ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa tcacacctat taccgggtgc 4980
aactggtagt taagagagct gcagctgccg tcatccctga gcaggggggc cacttcgtta 5040
agcatgtccc tgactcgcat gttttccctg accaaatccg ccagaaggcg ctcgccgccc 5100
agcgatagca gttcttgcaa ggaagcaaag tttttcaacg gtttgagacc gtccgccgta 5160
ggcatgcttt tgagcgtttg accaagcagt tccaggcggt cccacagctc ggtcacctgc 5220
tctacggcat ctcgatccag catatctcct cgtttcgcgg gttggggcgg ctttcgctgt 5280
acggcagtag tcggtgctcg tccagacggg ccagggtcat gtctttccac gggcgcaggg 5340
tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc tccgggctgc gcgctggcca 5400
gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt 5460
cggccaggta gcatttgacc atggtgtcat agtccagccc ctccgcggcg tggcccttgg 5520
cgcgcagctt gcccttggag gaggcgccgc acgaggggca gtgcagactt ttgagggcgt 5580
agagcttggg cgcgagaaat accgattccg gggagtaggc atccgcgccg caggccccgc 5640
agacggtctc gcattccacg agccaggtga gctctggccg ttcggggtca aaaaccaggt 5700
ttcccccatg ctttttgatg cgtttcttac ctctggtttc catgagccgg tgtccacgct 5760
cggtgacgaa aaggctgtcc gtgtccccgt atacagactt gagaggcctg tcctcgagcg 5820
gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacaaag gctcgcgtcc 5880
aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact agggggtcca 5940
ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag gtgattggtt 6000
tgtaggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag ggggtggggg 6060
cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt tggggtgagt 6120
actccctctg aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg 6180
aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgca tccatctggt 6240
cagaaaagac aatctttttg ttgtcaagct tggtggcaaa cgacccgtag agggcgttgg 6300
acagcaactt ggcgatggag cgcagggttt ggtttttgtc gcgatcggcg cgctccttgg 6360
ccgcgatgtt tagctgcacg tattcgcgcg caacgcaccg ccattcggga aagacggtgg 6420
tgcgctcgtc gggcaccagg tgcacgcgcc aaccgcggtt gtgcagggtg acaaggtcaa 6480
cgctggtggc tacctctccg cgtaggcgct cgttggtcca gcagaggcgg ccgcccttgc 6540
gcgagcagaa tggcggtagg gggtctagct gcgtctcgtc cggggggtct gcgtccacgg 6600
taaagacccc gggcagcagg cgcgcgtcga agtagtctat cttgcatcct tgcaagtcta 6660
gcgcctgctg ccatgcgcgg gcggcaagcg cgcgctcgta tgggttgagt gggggacccc 6720
atggcatggg gtgggtgagc gcggaggcgt acatgccgca aatgtcgtaa acgtagaggg 6780
gctctctgag tattccaaga tatgtagggt agcatcttcc accgcggatg ctggcgcgca 6840
cgtaatcgta tagttcgtgc gagggagcga ggaggtcggg accgaggttg ctacgggcgg 6900
gctgctctgc tcggaagact atctgcctga agatggcatg tgagttggat gatatggttg 6960
gacgctggaa gacgttgaag ctggcgtctg tgagacctac cgcgtcacgc acgaaggagg 7020
cgtaggagtc gcgcagcttg ttgaccagct cggcggtgac ctgcacgtct agggcgcagt 7080
agtccagggt ttccttgatg atgtcatact tatcctgtcc cttttttttc cacagctcgc 7140
ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct 7200
ccgaacggta agagcctagc atgtagaact ggttgacggc ctggtaggcg cagcatccct 7260
tttctacggg tagcgcgtat gcctgcgcgg ccttccggag cgaggtgtgg gtgagcgcaa 7320
aggtgtccct gaccatgact ttgaggtact ggtatttgaa gtcagtgtcg tcgcatccgc 7380
cctgctccca gagcaaaaag tccgtgcgct ttttggaacg cggatttggc agggcgaagg 7440
tgacatcgtt gaagagtatc tttcccgcgc gaggcataaa gttgcgtgtg atgcggaagg 7500
gtcccggcac ctcggaacgg ttgttaatta cctgggcggc gagcacgatc tcgtcaaagc 7560
cgttgatgtt gtggcccaca atgtaaagtt ccaagaagcg cgggatgccc ttgatggaag 7620
gcaatttttt aagttcctcg taggtgagct cttcagggga gctgagcccg tgctctgaaa 7680
gggcccagtc tgcaagatga gggttggaag cgacgaatga gctccacagg tcacgggcca 7740
ttagcatttg caggtggtcg cgaaaggtcc taaactggcg acctatggcc attttttctg 7800
gggtgatgca gtagaaggta agcgggtctt gttcccagcg gtcccatcca aggttcgcgg 7860
ctaggtctcg cgcggcagtc actagaggct catctccgcc gaacttcatg accagcatga 7920
agggcacgag ctgcttccca aaggccccca tccaagtata ggtctctaca tcgtaggtga 7980
caaagagacg ctcggtgcga ggatgcgagc cgatcgggaa gaactggatc tcccgccacc 8040
aattggagga gtggctattg atgtggtgaa agtagaagtc cctgcgacgg gccgaacact 8100
cgtgctggct tttgtaaaaa cgtgcgcagt actggcagcg gtgcacgggc tgtacatcct 8160
gcacgaggtt gacctgacga ccgcgcacaa ggaagcagag tgggaatttg agcccctcgc 8220
ctggcgggtt tggctggtgg tcttctactt cggctgcttg tccttgaccg tctggctgct 8280
cgaggggagt tacggtggat cggaccacca cgccgcgcga gcccaaagtc cagatgtccg 8340
cgcgcggcgg tcggagcttg atgacaacat cgcgcagatg ggagctgtcc atggtctgga 8400
gctcccgcgg cgtcaggtca ggcgggagct cctgcaggtt tacctcgcat agacgggtca 8460
gggcgcgggc tagatccagg tgatacctaa tttccagggg ctggttggtg gcggcgtcga 8520
tggcttgcaa gaggccgcat ccccgcggcg cgactacggt accgcgcggc gggcggtggg 8580
ccgcgggggt gtccttggat gatgcatcta aaagcggtga cgcgggcgag cccccggagg 8640
tagggggggc tccggacccg ccgggagagg gggcaggggc acgtcggcgc cgcgcgcggg 8700
caggagctgg tgctgcgcgc gtaggttgct ggcgaacgcg acgacgcggc ggttgatctc 8760
ctgaatctgg cgcctctgcg tgaagacgac gggcccggtg agcttgagcc tgaaagagag 8820
ttcgacagaa tcaatttcgg tgtcgttgac ggcggcctgg cgcaaaatct cctgcacgtc 8880
tcctgagttg tcttgatagg cgatctcggc catgaactgc tcgatctctt cctcctggag 8940
atctccgcgt ccggctcgct ccacggtggc ggcgaggtcg ttggaaatgc gggccatgag 9000
ctgcgagaag gcgttgaggc ctccctcgtt ccagacgcgg ctgtagacca cgcccccttc 9060
ggcatcgcgg gcgcgcatga ccacctgcgc gagattgagc tccacgtgcc gggcgaagac 9120
ggcgtagttt cgcaggcgct gaaagaggta gttgagggtg gtggcggtgt gttctgccac 9180
gaagaagtac ataacccagc gtcgcaacgt ggattcgttg atatccccca aggcctcaag 9240
gcgctccatg gcctcgtaga agtccacggc gaagttgaaa aactgggagt tgcgcgccga 9300
cacggttaac tcctcctcca gaagacggat gagctcggcg acagtgtcgc gcacctcgcg 9360
ctcaaaggct acaggggcct cttcttcttc ttcaatctcc tcttccataa gggcctcccc 9420
ttcttcttct tctggcggcg gtgggggagg ggggacacgg cggcgacgac ggcgcaccgg 9480
gaggcggtcg acaaagcgct cgatcatctc cccgcggcga cggcgcatgg tctcggtgac 9540
ggcgcggccg ttctcgcggg ggcgcagttg gaagacgccg cccgtcatgt cccggttatg 9600
ggttggcggg gggctgccat gcggcaggga tacggcgcta acgatgcatc tcaacaattg 9660
ttgtgtaggt actccgccgc cgagggacct gagcgagtcc gcatcgaccg gatcggaaaa 9720
cctctcgaga aaggcgtcta accagtcaca gtcgcaaggt aggctgagca ccgtggcggg 9780
cggcagcggg cggcggtcgg ggttgtttct ggcggaggtg ctgctgatga tgtaattaaa 9840
gtaggcggtc ttgagacggc ggatggtcga cagaagcacc atgtccttgg gtccggcctg 9900
ctgaatgcgc aggcggtcgg ccatgcccca ggcttcgttt tgacatcggc gcaggtcttt 9960
gtagtagtct tgcatgagcc tttctaccgg cacttcttct tctccttcct cttgtcctgc 10020
atctcttgca tctatcgctg cggcggcggc ggagtttggc cgtaggtggc gccctcttcc 10080
tcccatgcgt gtgaccccga agcccctcat cggctgaagc agggctaggt cggcgacaac 10140
gcgctcggct aatatggcct gctgcacctg cgtgagggta gactggaagt catccatgtc 10200
cacaaagcgg tggtatgcgc ccgtgttgat ggtgtaagtg cagttggcca taacggacca 10260
gttaacggtc tggtgacccg gctgcgagag ctcggtgtac ctgagacgcg agtaagccct 10320
cgagtcaaat acgtagtcgt tgcaagtccg caccaggtac tggtatccca ccaaaaagtg 10380
cggcggcggc tggcggtaga ggggccagcg tagggtggcc ggggctccgg gggcgagatc 10440
ttccaacata aggcgatgat atccgtagat gtacctggac atccaggtga tgccggcggc 10500
ggtggtggag gcgcgcggaa agtcgcggac gcggttccag atgttgcgca gcggcaaaaa 10560
gtgctccatg gtcgggacgc tctggccggt caggcgcgcg caatcgttga cgctctagac 10620
cgtgcaaaag gagagcctgt aagcgggcac tcttccgtgg tctggtggat aaattcgcaa 10680
gggtatcatg gcggacgacc ggggttcgag ccccgtatcc ggccgtccgc cgtgatccat 10740
gcggttaccg cccgcgtgtc gaacccaggt gtgcgacgtc agacaacggg ggagtgctcc 10800
ttttggcttc cttccaggcg cggcggctgc tgcgctagct tttttggcca ctggccgcgc 10860
gcagcgtaag cggttaggct ggaaagcgaa agcattaagt ggctcgctcc ctgtagccgg 10920
agggttattt tccaagggtt gagtcgcggg acccccggtt cgagtctcgg accggccgga 10980
ctgcggcgaa cgggggtttg cctccccgtc atgcaagacc ccgcttgcaa attcctccgg 11040
aaacagggac gagccccttt tttgcttttc ccagatgcat ccggtgctgc ggcagatgcg 11100
cccccctcct cagcagcggc aagagcaaga gcagcggcag acatgcaggg caccctcccc 11160
tcctcctacc gcgtcaggag gggcgacatc cgcggttgac gcggcagcag atggtgatta 11220
cgaacccccg cggcgccggg cccggcacta cctggacttg gaggagggcg agggcctggc 11280
gcggctagga gcgccctctc ctgagcggta cccaagggtg cagctgaagc gtgatacgcg 11340
tgaggcgtac gtgccgcggc agaacctgtt tcgcgaccgc gagggagagg agcccgagga 11400
gatgcgggat cgaaagttcc acgcagggcg cgagctgcgg catggcctga atcgcgagcg 11460
gttgctgcgc gaggaggact ttgagcccga cgcgcgaacc gggattagtc ccgcgcgcgc 11520
acacgtggcg gccgccgacc tggtaaccgc atacgagcag acggtgaacc aggagattaa 11580
ctttcaaaaa agctttaaca accacgtgcg tacgcttgtg gcgcgcgagg aggtggctat 11640
aggactgatg catctgtggg actttgtaag cgcgctggag caaaacccaa atagcaagcc 11700
gctcatggcg cagctgttcc ttatagtgca gcacagcagg gacaacgagg cattcaggga 11760
tgcgctgcta aacatagtag agcccgaggg ccgctggctg ctcgatttga taaacatcct 11820
gcagagcata gtggtgcagg agcgcagctt gagcctggct gacaaggtgg ccgccatcaa 11880
ctattccatg cttagcctgg gcaagtttta cgcccgcaag atataccata ccccttacgt 11940
tcccatagac aaggaggtaa agatcgaggg gttctacatg cgcatggcgc tgaaggtgct 12000
taccttgagc gacgacctgg gcgtttatcg caacgagcgc atccacaagg ccgtgagcgt 12060
gagccggcgg cgcgagctca gcgaccgcga gctgatgcac agcctgcaaa gggccctggc 12120
tggcacgggc agcggcgata gagaggccga gtcctacttt gacgcgggcg ctgacctgcg 12180
ctgggcccca agccgacgcg ccctggaggc agctggggcc ggacctgggc tggcggtggc 12240
acccgcgcgc gctggcaacg tcggcggcgt ggaggaatat gacgaggacg atgagtacga 12300
gccagaggac ggcgagtact aagcggtgat gtttctgatc agatgatgca agacgcaacg 12360
gacccggcgg tgcgggcggc gctgcagagc cagccgtccg gccttaactc cacggacgac 12420
tggcgccagg tcatggaccg catcatgtcg ctgactgcgc gcaatcctga cgcgttccgg 12480
cagcagccgc aggccaaccg gctctccgca attctggaag cggtggtccc ggcgcgcgca 12540
aaccccacgc acgagaaggt gctggcgatc gtaaacgcgc tggccgaaaa cagggccatc 12600
cggcccgacg aggccggcct ggtctacgac gcgctgcttc agcgcgtggc tcgttacaac 12660
agcggcaacg tgcagaccaa cctggaccgg ctggtggggg atgtgcgcga ggccgtggcg 12720
cagcgtgagc gcgcgcagca gcagggcaac ctgggctcca tggttgcact aaacgccttc 12780
ctgagtacac agcccgccaa cgtgccgcgg ggacaggagg actacaccaa ctttgtgagc 12840
gcactgcggc taatggtgac tgagacaccg caaagtgagg tgtaccagtc tgggccagac 12900
tattttttcc agaccagtag acaaggcctg cagaccgtaa acctgagcca ggctttcaaa 12960
aacttgcagg ggctgtgggg ggtgcgggct cccacaggcg accgcgcgac cgtgtctagc 13020
ttgctgacgc ccaactcgcg cctgttgctg ctgctaatag cgcccttcac ggacagtggc 13080
agcgtgtccc gggacacata cctaggtcac ttgctgacac tgtaccgcga ggccataggt 13140
caggcgcatg tggacgagca tactttccag gagattacaa gtgtcagccg cgcgctgggg 13200
caggaggaca cgggcagcct ggaggcaacc ctaaactacc tgctgaccaa ccggcggcag 13260
aagatcccct cgttgcacag tttaaacagc gaggaggagc gcattttgcg ctacgtgcag 13320
cagagcgtga gccttaacct gatgcgcgac ggggtaacgc ccagcgtggc gctggacatg 13380
accgcgcgca acatggaacc gggcatgtat gcctcaaacc ggccgtttat caaccgccta 13440
atggactact tgcatcgcgc ggccgccgtg aaccccgagt atttcaccaa tgccatcttg 13500
aacccgcact ggctaccgcc ccctggtttc tacaccgggg gattcgaggt gcccgagggt 13560
aacgatggat tcctctggga cgacatagac gacagcgtgt tttccccgca accgcagacc 13620
ctgctagagt tgcaacagcg cgagcaggca gaggcggcgc tgcgaaagga aagcttccgc 13680
aggccaagca gcttgtccga tctaggcgct gcggccccgc ggtcagatgc tagtagccca 13740
tttccaagct tgatagggtc tcttaccagc actcgcacca cccgcccgcg cctgctgggc 13800
gaggaggagt acctaaacaa ctcgctgctg cagccgcagc gcgaaaaaaa cctgcctccg 13860
gcatttccca acaacgggat agagagccta gtggacaaga tgagtagatg gaagacgtac 13920
gcgcaggagc acagggacgt gccaggcccg cgcccgccca cccgtcgtca aaggcacgac 13980
cgtcagcggg gtctggtgtg ggaggacgat gactcggcag acgacagcag cgtcctggat 14040
ttgggaggga gtggcaaccc gtttgcgcac cttcgcccca ggctggggag aatgttttaa 14100
aaaaaaaaaa gcatgatgca aaataaaaaa ctcaccaagg ccatggcacc gagcgttggt 14160
tttcttgtat tccccttagt atgcggcgcg cggcgatgta tgaggaaggt cctcctccct 14220
cctacgagag tgtggtgagc gcggcgccag tggcggcggc gctgggttct cccttcgatg 14280
ctcccctgga cccgccgttt gtgcctccgc ggtacctgcg gcctaccggg gggagaaaca 14340
gcatccgtta ctctgagttg gcacccctat tcgacaccac ccgtgtgtac ctggtggaca 14400
acaagtcaac ggatgtggca tccctgaact accagaacga ccacagcaac tttctgacca 14460
cggtcattca aaacaatgac tacagcccgg gggaggcaag cacacagacc atcaatcttg 14520
acgaccggtc gcactggggc ggcgacctga aaaccatcct gcataccaac atgccaaatg 14580
tgaacgagtt catgtttacc aataagttta aggcgcgggt gatggtgtcg cgcttgccta 14640
ctaaggacaa tcaggtggag ctgaaatacg agtgggtgga gttcacgctg cccgagggca 14700
actactccga gaccatgacc atagacctta tgaacaacgc gatcgtggag cactacttga 14760
aagtgggcag acagaacggg gttctggaaa gcgacatcgg ggtaaagttt gacacccgca 14820
acttcagact ggggtttgac cccgtcactg gtcttgtcat gcctggggta tatacaaacg 14880
aagccttcca tccagacatc attttgctgc caggatgcgg ggtggacttc acccacagcc 14940
gcctgagcaa cttgttgggc atccgcaagc ggcaaccctt ccaggagggc tttaggatca 15000
cctacgatga tctggagggt ggtaacattc ccgcactgtt ggatgtggac gcctaccagg 15060
cgagcttgaa agatgacacc gaacagggcg ggggtggcgc aggcggcagc aacagcagtg 15120
gcagcggcgc ggaagagaac tccaacgcgg cagccgcggc aatgcagccg gtggaggaca 15180
tgaacgatca tgccattcgc ggcgacacct ttgccacacg ggctgaggag aagcgcgctg 15240
aggccgaagc agcggccgaa gctgccgccc ccgctgcgca acccgaggtc gagaagcctc 15300
agaagaaacc ggtgatcaaa cccctgacag aggacagcaa gaaacgcagt tacaacctaa 15360
taagcaatga cagcaccttc acccagtacc gcagctggta ccttgcatac aactacggcg 15420
accctcagac cggaatccgc tcatggaccc tgctttgcac tcctgacgta acctgcggct 15480
cggagcaggt ctactggtcg ttgccagaca tgatgcaaga ccccgtgacc ttccgctcca 15540
cgcgccagat cagcaacttt ccggtggtgg gcgccgagct gttgcccgtg cactccaaga 15600
gcttctacaa cgaccaggcc gtctactccc aactcatccg ccagtttacc tctctgaccc 15660
acgtgttcaa tcgctttccc gagaaccaga ttttggcgcg cccgccagcc cccaccatca 15720
ccaccgtcag tgaaaacgtt cctgctctca cagatcacgg gacgctaccg ctgcgcaaca 15780
gcatcggagg agtccagcga gtgaccatta ctgacgccag acgccgcacc tgcccctacg 15840
tttacaaggc cctgggcata gtctcgccgc gcgtcctatc gagccgcact ttttgagcaa 15900
gcatgtccat ccttatatcg cccagcaata acacaggctg gggcctgcgc ttcccaagca 15960
agatgtttgg cggggccaag aagcgctccg accaacaccc agtgcgcgtg cgcgggcact 16020
accgcgcgcc ctggggcgcg cacaaacgcg gccgcactgg gcgcaccacc gtcgatgacg 16080
ccatcgacgc ggtggtggag gaggcgcgca actacacgcc cacgccgcca ccagtgtcca 16140
cagtggacgc ggccattcag accgtggtgc gcggagcccg gcgctatgct aaaatgaaga 16200
gacggcggag gcgcgtagca cgtcgccacc gccgccgacc cggcactgcc gcccaacgcg 16260
cggcggcggc cctgcttaac cgcgcacgtc gcaccggccg acgggcggcc atgcgggccg 16320
ctcgaaggct ggccgcgggt attgtcactg tgccccccag gtccaggcga cgagcggccg 16380
ccgcagcagc cgcggccatt agtgctatga ctcagggtcg caggggcaac gtgtattggg 16440
tgcgcgactc ggttagcggc ctgcgcgtgc ccgtgcgcac ccgccccccg cgcaactaga 16500
ttgcaagaaa aaactactta gactcgtact gttgtatgta tccagcggcg gcggcgcgca 16560
acgaagctat gtccaagcgc aaaatcaaag aagagatgct ccaggtcatc gcgccggaga 16620
tctatggccc cccgaagaag gaagagcagg attacaagcc ccgaaagcta aagcgggtca 16680
aaaagaaaaa gaaagatgat gatgatgaac ttgacgacga ggtggaactg ctgcacgcta 16740
ccgcgcccag gcgacgggta cagtggaaag gtcgacgcgt aaaacgtgtt ttgcgacccg 16800
gcaccaccgt agtctttacg cccggtgagc gctccacccg cacctacaag cgcgtgtatg 16860
atgaggtgta cggcgacgag gacctgcttg agcaggccaa cgagcgcctc ggggagtttg 16920
cctacggaaa gcggcataag gacatgctgg cgttgccgct ggacgagggc aacccaacac 16980
ctagcctaaa gcccgtaaca ctgcagcagg tgctgcccgc gcttgcaccg tccgaagaaa 17040
agcgcggcct aaagcgcgag tctggtgact tggcacccac cgtgcagctg atggtaccca 17100
agcgccagcg actggaagat gtcttggaaa aaatgaccgt ggaacctggg ctggagcccg 17160
aggtccgcgt gcggccaatc aagcaggtgg cgccgggact gggcgtgcag accgtggacg 17220
ttcagatacc cactaccagt agcaccagta ttgccaccgc cacagagggc atggagacac 17280
aaacgtcccc ggttgcctca gcggtggcgg atgccgcggt gcaggcggtc gctgcggccg 17340
cgtccaagac ctctacggag gtgcaaacgg acccgtggat gtttcgcgtt tcagcccccc 17400
ggcgcccgcg cggttcgagg aagtacggcg ccgccagcgc gctactgccc gaatatgccc 17460
tacatccttc cattgcgcct acccccggct atcgtggcta cacctaccgc cccagaagac 17520
gagcaactac ccgacgccga accaccactg gaacccgccg ccgccgtcgc cgtcgccagc 17580
ccgtgctggc cccgatttcc gtgcgcaggg tggctcgcga aggaggcagg accctggtgc 17640
tgccaacagc gcgctaccac cccagcatcg tttaaaagcc ggtctttgtg gttcttgcag 17700
atatggccct cacctgccgc ctccgtttcc cggtgccggg attccgagga agaatgcacc 17760
gtaggagggg catggccggc cacggcctga cgggcggcat gcgtcgtgcg caccaccggc 17820
ggcggcgcgc gtcgcaccgt cgcatgcgcg gcggtatcct gcccctcctt attccactga 17880
tcgccgcggc gattggcgcc gtgcccggaa ttgcatccgt ggccttgcag gcgcagagac 17940
actgattaaa aacaagttgc atgtggaaaa atcaaaataa aaagtctgga ctctcacgct 18000
cgcttggtcc tgtaactatt ttgtagaatg gaagacatca actttgcgtc tctggccccg 18060
cgacacggct cgcgcccgtt catgggaaac tggcaagata tcggcaccag caatatgagc 18120
ggtggcgcct tcagctgggg ctcgctgtgg agcggcatta aaaatttcgg ttccaccgtt 18180
aagaactatg gcagcaaggc ctggaacagc agcacaggcc agatgctgag ggataagttg 18240
aaagagcaaa atttccaaca aaaggtggta gatggcctgg cctctggcat tagcggggtg 18300
gtggacctgg ccaaccaggc agtgcaaaat aagattaaca gtaagcttga tccccgccct 18360
cccgtagagg agcctccacc ggccgtggag acagtgtctc cagaggggcg tggcgaaaag 18420
cgtccgcgcc ccgacaggga agaaactctg gtgacgcaaa tagacgagcc tccctcgtac 18480
gaggaggcac taaagcaagg cctgcccacc acccgtccca tcgcgcccat ggctaccgga 18540
gtgctgggcc agcacacacc cgtaacgctg gacctgcctc cccccgccga cacccagcag 18600
aaacctgtgc tgccaggccc gaccgccgtt gttgtaaccc gtcctagccg cgcgtccctg 18660
cgccgcgccg ccagcggtcc gcgatcgttg cggcccgtag ccagtggcaa ctggcaaagc 18720
acactgaaca gcatcgtggg tctgggggtg caatccctga agcgccgacg atgcttctga 18780
atagctaacg tgtcgtatgt gtgtcatgta tgcgtccatg tcgccgccag aggagctgct 18840
gagccgccgc gcgcccgctt tccaagatgg ctaccccttc gatgatgccg cagtggtctt 18900
acatgcacat ctcgggccag gacgcctcgg agtacctgag ccccgggctg gtgcagtttg 18960
cccgcgccac cgagacgtac ttcagcctga ataacaagtt tagaaacccc acggtggcgc 19020
ctacgcacga cgtgaccaca gaccggtccc agcgtttgac gctgcggttc atccctgtgg 19080
accgtgagga tactgcgtac tcgtacaagg cgcggttcac cctagctgtg ggtgataacc 19140
gtgtgctgga catggcttcc acgtactttg acatccgcgg cgtgctggac aggggcccta 19200
cttttaagcc ctactctggc actgcctaca acgccctggc tcccaagggt gccccaaatc 19260
cttgcgaatg ggatgaagct gctactgctc ttgaaataaa cctagaagaa gaggacgatg 19320
acaacgaaga cgaagtagac gagcaagctg agcagcaaaa aactcacgta tttgggcagg 19380
cgccttattc tggtataaat attacaaagg agggtattca aataggtgtc gaaggtcaaa 19440
cacctaaata tgccgataaa acatttcaac ctgaacctca aataggagaa tctcagtggt 19500
acgaaactga aattaatcat gcagctggga gagtccttaa aaagactacc ccaatgaaac 19560
catgttacgg ttcatatgca aaacccacaa atgaaaatgg agggcaaggc attcttgtaa 19620
agcaacaaaa tggaaagcta gaaagtcaag tggaaatgca atttttctca actactgagg 19680
cgaccgcagg caatggtgat aacttgactc ctaaagtggt attgtacagt gaagatgtag 19740
atatagaaac cccagacact catatttctt acatgcccac tattaaggaa ggtaactcac 19800
gagaactaat gggccaacaa tctatgccca acaggcctaa ttacattgct tttagggaca 19860
attttattgg tctaatgtat tacaacagca cgggtaatat gggtgttctg gcgggccaag 19920
catcgcagtt gaatgctgtt gtagatttgc aagacagaaa cacagagctt tcataccagc 19980
ttttgcttga ttccattggt gatagaacca ggtacttttc tatgtggaat caggctgttg 20040
acagctatga tccagatgtt agaattattg aaaatcatgg aactgaagat gaacttccaa 20100
attactgctt tccactggga ggtgtgatta atacagagac tcttaccaag gtaaaaccta 20160
aaacaggtca ggaaaatgga tgggaaaaag atgctacaga attttcagat aaaaatgaaa 20220
taagagttgg aaataatttt gccatggaaa tcaatctaaa tgccaacctg tggagaaatt 20280
tcctgtactc caacatagcg ctgtatttgc ccgacaagct aaagtacagt ccttccaacg 20340
taaaaatttc tgataaccca aacacctacg actacatgaa caagcgagtg gtggctcccg 20400
ggttagtgga ctgctacatt aaccttggag cacgctggtc ccttgactat atggacaacg 20460
tcaacccatt taaccaccac cgcaatgctg gcctgcgcta ccgctcaatg ttgctgggca 20520
atggtcgcta tgtgcccttc cacatccagg tgcctcagaa gttctttgcc attaaaaacc 20580
tccttctcct gccgggctca tacacctacg agtggaactt caggaaggat gttaacatgg 20640
ttctgcagag ctccctagga aatgacctaa gggttgacgg agccagcatt aagtttgata 20700
gcatttgcct ttacgccacc ttcttcccca tggcccacaa caccgcctcc acgcttgagg 20760
ccatgcttag aaacgacacc aacgaccagt cctttaacga ctatctctcc gccgccaaca 20820
tgctctaccc tatacccgcc aacgctacca acgtgcccat atccatcccc tcccgcaact 20880
gggcggcttt ccgcggctgg gccttcacgc gccttaagac taaggaaacc ccatcactgg 20940
gctcgggcta cgacccttat tacacctact ctggctctat accctaccta gatggaacct 21000
tttacctcaa ccacaccttt aagaaggtgg ccattacctt tgactcttct gtcagctggc 21060
ctggcaatga ccgcctgctt acccccaacg agtttgaaat taagcgctca gttgacgggg 21120
agggttacaa cgttgcccag tgtaacatga ccaaagactg gttcctggta caaatgctag 21180
ctaactacaa cattggctac cagggcttct atatcccaga gagctacaag gaccgcatgt 21240
actccttctt tagaaacttc cagcccatga gccgtcaggt ggtggatgat actaaataca 21300
aggactacca acaggtgggc atcctacacc aacacaacaa ctctggattt gttggctacc 21360
ttgcccccac catgcgcgaa ggacaggcct accctgctaa cttcccctat ccgcttatag 21420
gcaagaccgc agttgacagc attacccaga aaaagtttct ttgcgatcgc accctttggc 21480
gcatcccatt ctccagtaac tttatgtcca tgggcgcact cacagacctg ggccaaaacc 21540
ttctctacgc caactccgcc cacgcgctag acatgacttt tgaggtggat cccatggacg 21600
agcccaccct tctttatgtt ttgtttgaag tctttgacgt ggtccgtgtg caccggccgc 21660
accgcggcgt catcgaaacc gtgtacctgc gcacgccctt ctcggccggc aacgccacaa 21720
cataaagaag caagcaacat caacaacagc tgccgccatg ggctccagtg agcaggaact 21780
gaaagccatt gtcaaagatc ttggttgtgg gccatatttt ttgggcacct atgacaagcg 21840
ctttccaggc tttgtttctc cacacaagct cgcctgcgcc atagtcaata cggccggtcg 21900
cgagactggg ggcgtacact ggatggcctt tgcctggaac ccgcactcaa aaacatgcta 21960
cctctttgag ccctttggct tttctgacca gcgactcaag caggtttacc agtttgagta 22020
cgagtcactc ctgcgccgta gcgccattgc ttcttccccc gaccgctgta taacgctgga 22080
aaagtccacc caaagcgtac aggggcccaa ctcggccgcc tgtggactat tctgctgcat 22140
gtttctccac gcctttgcca actggcccca aactcccatg gatcacaacc ccaccatgaa 22200
ccttattacc ggggtaccca actccatgct caacagtccc caggtacagc ccaccctgcg 22260
tcgcaaccag gaacagctct acagcttcct ggagcgccac tcgccctact tccgcagcca 22320
cagtgcgcag attaggagcg ccacttcttt ttgtcacttg aaaaacatgt aaaaataatg 22380
tactagagac actttcaata aaggcaaatg cttttatttg tacactctcg ggtgattatt 22440
tacccccacc cttgccgtct gcgccgttta aaaatcaaag gggttctgcc gcgcatcgct 22500
atgcgccact ggcagggaca cgttgcgata ctggtgttta gtgctccact taaactcagg 22560
cacaaccatc cgcggcagct cggtgaagtt ttcactccac aggctgcgca ccatcaccaa 22620
cgcgtttagc aggtcgggcg ccgatatctt gaagtcgcag ttggggcctc cgccctgcgc 22680
gcgcgagttg cgatacacag ggttgcagca ctggaacact atcagcgccg ggtggtgcac 22740
gctggccagc acgctcttgt cggagatcag atccgcgtcc aggtcctccg cgttgctcag 22800
ggcgaacgga gtcaactttg gtagctgcct tcccaaaaag ggcgcgtgcc caggctttga 22860
gttgcactcg caccgtagtg gcatcaaaag gtgaccgtgc ccggtctggg cgttaggata 22920
cagcgcctgc ataaaagcct tgatctgctt aaaagccacc tgagcctttg cgccttcaga 22980
gaagaacatg ccgcaagact tgccggaaaa ctgattggcc ggacaggccg cgtcgtgcac 23040
gcagcacctt gcgtcggtgt tggagatctg caccacattt cggccccacc ggttcttcac 23100
gatcttggcc ttgctagact gctccttcag cgcgcgctgc ccgttttcgc tcgtcacatc 23160
catttcaatc acgtgctcct tatttatcat aatgcttccg tgtagacact taagctcgcc 23220
ttcgatctca gcgcagcggt gcagccacaa cgcgcagccc gtgggctcgt gatgcttgta 23280
ggtcacctct gcaaacgact gcaggtacgc ctgcaggaat cgccccatca tcgtcacaaa 23340
ggtcttgttg ctggtgaagg tcagctgcaa cccgcggtgc tcctcgttca gccaggtctt 23400
gcatacggcc gccagagctt ccacttggtc aggcagtagt ttgaagttcg cctttagatc 23460
gttatccacg tggtacttgt ccatcagcgc gcgcgcagcc tccatgccct tctcccacgc 23520
agacacgatc ggcacactca gcgggttcat caccgtaatt tcactttccg cttcgctggg 23580
ctcttcctct tcctcttgcg tccgcatacc acgcgccact gggtcgtctt cattcagccg 23640
ccgcactgtg cgcttacctc ctttgccatg cttgattagc accggtgggt tgctgaaacc 23700
caccatttgt agcgccacat cttctctttc ttcctcgctg tccacgatta cctctggtga 23760
tggcgggcgc tcgggcttgg gagaagggcg cttctttttc ttcttgggcg caatggccaa 23820
atccgccgcc gaggtcgatg gccgcgggct gggtgtgcgc ggcaccagcg cgtcttgtga 23880
tgagtcttcc tcgtcctcgg actcgatacg ccgcctcatc cgcttttttg ggggcgcccg 23940
gggaggcggc ggcgacgggg acggggacga cacgtcctcc atggttgggg gacgtcgcgc 24000
cgcaccgcgt ccgcgctcgg gggtggtttc gcgctgctcc tcttcccgac tggccatttc 24060
cttctcctat aggcagaaaa agatcatgga gtcagtcgag aagaaggaca gcctaaccgc 24120
cccctctgag ttcgccacca ccgcctccac cgatgccgcc aacgcgccta ccaccttccc 24180
cgtcgaggca cccccgcttg aggaggagga agtgattatc gagcaggacc caggttttgt 24240
aagcgaagac gacgaggacc gctcagtacc aacagaggat aaaaagcaag accaggacaa 24300
cgcagaggca aacgaggaac aagtcgggcg gggggacgaa aggcatggcg actacctaga 24360
tgtgggagac gacgtgctgt tgaagcatct gcagcgccag tgcgccatta tctgcgacgc 24420
gttgcaagag cgcagcgatg tgcccctcgc catagcggat gtcagccttg cctacgaacg 24480
ccacctattc tcaccgcgcg taccccccaa acgccaagaa aacggcacat gcgagcccaa 24540
cccgcgcctc aacttctacc ccgtatttgc cgtgccagag gtgcttgcca cctatcacat 24600
ctttttccaa aactgcaaga tacccctatc ctgccgtgcc aaccgcagcc gagcggacaa 24660
gcagctggcc ttgcggcagg gcgctgtcat acctgatatc gcctcgctca acgaagtgcc 24720
aaaaatcttt gagggtcttg gacgcgacga gaagcgcgcg gcaaacgctc tgcaacagga 24780
aaacagcgaa aatgaaagtc actctggagt gttggtggaa ctcgagggtg acaacgcgcg 24840
cctagccgta ctaaaacgca gcatcgaggt cacccacttt gcctacccgg cacttaacct 24900
accccccaag gtcatgagca cagtcatgag tgagctgatc gtgcgccgtg cgcagcccct 24960
ggagagggat gcaaatttgc aagaacaaac agaggagggc ctacccgcag ttggcgacga 25020
gcagctagcg cgctggcttc aaacgcgcga gcctgccgac ttggaggagc gacgcaaact 25080
aatgatggcc gcagtgctcg ttaccgtgga gcttgagtgc atgcagcggt tctttgctga 25140
cccggagatg cagcgcaagc tagaggaaac attgcactac acctttcgac agggctacgt 25200
acgccaggcc tgcaagatct ccaacgtgga gctctgcaac ctggtctcct accttggaat 25260
tttgcacgaa aaccgccttg ggcaaaacgt gcttcattcc acgctcaagg gcgaggcgcg 25320
ccgcgactac gtccgcgact gcgtttactt atttctatgc tacacctggc agacggccat 25380
gggcgtttgg cagcagtgct tggaggagtg caacctcaag gagctgcaga aactgctaaa 25440
gcaaaacttg aaggacctat ggacggcctt caacgagcgc tccgtggccg cgcacctggc 25500
ggacatcatt ttccccgaac gcctgcttaa aaccctgcaa cagggtctgc cagacttcac 25560
cagtcaaagc atgttgcaga actttaggaa ctttatccta gagcgctcag gaatcttgcc 25620
cgccacctgc tgtgcacttc ctagcgactt tgtgcccatt aagtaccgcg aatgccctcc 25680
gccgctttgg ggccactgct accttctgca gctagccaac taccttgcct accactctga 25740
cataatggaa gacgtgagcg gtgacggtct actggagtgt cactgtcgct gcaacctatg 25800
caccccgcac cgctccctgg tttgcaattc gcagctgctt aacgaaagtc aaattatcgg 25860
tacctttgag ctgcagggtc cctcgcctga cgaaaagtcc gcggctccgg ggttgaaact 25920
cactccgggg ctgtggacgt cggcttacct tcgcaaattt gtacctgagg actaccacgc 25980
ccacgagatt aggttctacg aagaccaatc ccgcccgcca aatgcggagc ttaccgcctg 26040
cgtcattacc cagggccaca ttcttggcca attgcaagcc atcaacaaag cccgccaaga 26100
gtttctgcta cgaaagggac ggggggttta cttggacccc cagtccggcg aggagctcaa 26160
cccaatcccc ccgccgccgc agccctatca gcagcagccg cgggcccttg cttcccagga 26220
tggcacccaa aaagaagctg cagctgccgc cgccacccac ggacgaggag gaatactggg 26280
acagtcaggc agaggaggtt ttggacgagg aggaggagga catgatggaa gactgggaga 26340
gcctagacga ggaagcttcc gaggtcgaag aggtgtcaga cgaaacaccg tcaccctcgg 26400
tcgcattccc ctcgccggcg ccccagaaat cggcaaccgg ttccagcatg gctacaacct 26460
ccgctcctca ggcgccgccg gcactgcccg ttcgccgacc caaccgtaga tgggacacca 26520
ctggaaccag ggccggtaag tccaagcagc cgccgccgtt agcccaagag caacaacagc 26580
gccaaggcta ccgctcatgg cgcgggcaca agaacgccat agttgcttgc ttgcaagact 26640
gtgggggcaa catctccttc gcccgccgct ttcttctcta ccatcacggc gtggccttcc 26700
cccgtaacat cctgcattac taccgtcatc tctacagccc atactgcacc ggcggcagcg 26760
gcagcggcag caacagcagc ggccacacag aagcaaaggc gaccggatag caagactctg 26820
acaaagccca agaaatccac agcggcggca gcagcaggag gaggagcgct gcgtctggcg 26880
cccaacgaac ccgtatcgac ccgcgagctt agaaacagga tttttcccac tctgtatgct 26940
atatttcaac agagcagggg ccaagaacaa gagctgaaaa taaaaaacag gtctctgcga 27000
tccctcaccc gcagctgcct gtatcacaaa agcgaagatc agcttcggcg cacgctggaa 27060
gacgcggagg ctctcttcag taaatactgc gcgctgactc ttaaggacta gtttcgcgcc 27120
ctttctcaaa tttaagcgcg aaaactacgt catctccagc ggccacaccc ggcgccagca 27180
cctgtcgtca gcgccatttc aactttgtat acaaaagttg tgatgagcaa ggaaattccc 27240
acgccctaca tgtggagtta ccagccacaa atgggacttg cggctggagc tgcccaagac 27300
tactcaaccc gaataaacta catgagcgcg ggaccccaca tgatatcccg ggtcaacgga 27360
atacgcgccc accgaaaccg aattctcctg gaacaggcgg ctattaccac cacacctcgt 27420
aataacctta atccccgtag ttggcccgct gccctggtgt accaggaaag tcccgctccc 27480
accactgtgg tacttcccag agacgcccag gccgaagttc agatgactaa ctcaggggcg 27540
cagcttgcgg gcggctttcg tcacagggtg cggtcgcccg ggcagggtat aactcacctg 27600
acaatcagag ggcgaggtat tcagctcaac gacgagtcgg tgagctcctc gcttggtctc 27660
cgtccggacg ggacatttca gatcggcggc gccggccgct cttcattcac gcctcgtcag 27720
gcaatcctaa ctctgcagac ctcgtcctct gagccgcgct ctggaggcat tggaactctg 27780
caatttattg aggagtttgt gccatcggtc tactttaacc ccttctcggg acctcccggc 27840
cactatccgg atcaatttat tcctaacttt gacgcggtaa aggactcggc ggacggctac 27900
gactgaatgt taagtggaga ggcagagcaa ctgcgcctga aacacctggt ccactgtcgc 27960
cgccacaagt gctttgcccg cgactccggt gagttttgct actttgaatt gcccgaggat 28020
catatcgagg gcccggcgca cggcgtccgg cttaccgccc agggagagct tgcccgtagc 28080
ctgattcggg agtttaccca gcgccccctg ctagttgagc gggacagggg accctgtgtt 28140
ctcactgtga tttgcaactg tcctaaccct ggattacatc aagatctttg ttgccatctc 28200
tgtgctgagt ataataaata cagaaattaa aatatactgg ggctcctatc gccatcctgt 28260
aaacgccacc gtcttcaccc gcccaagcaa accaaggcga accttacctg gtacttttaa 28320
catctctccc tctgtgattt acaacagttt caacccagac ggagtgagtc tacgagagaa 28380
cctctccgag ctcagctact ccatcagaaa aaacaccacc ctccttacct gccgggaacg 28440
tacgagtgcg tcaccggccg ctgcaccaca cctaccgcct gaccgtaaac cagacttttt 28500
ccggacagac ctcaataact ctgtttacca gaacaggagg tgagcttaga aaacccttag 28560
ggtattaggc caaaggcgca gctactgtgg ggtttatgaa caattcaagc aactctacgg 28620
gctattctaa ttcaggtttc tctagaatcg gggttggggt tattctctgt cttgtgattc 28680
tctttattct tatactaacg cttctctgcc taaggctcgc cgcctgctgt gtgcacattt 28740
gcatttattg tcagcttttt aaacgctggg gtcgccaccc aagatgatta ggtacataat 28800
cctaggttta ctcacccttg cgtcagccca cggtaccacc caaaaggtgg attttaagga 28860
gccagcctgt aatgttacat tcgcagctga agctaatgag tgcaccactc ttataaaatg 28920
caccacagaa catgaaaagc tgcttattcg ccacaaaaac aaaattggca agtatgctgt 28980
ttatgctatt tggcagccag gtgacactac agagtataat gttacagttt tccagggtaa 29040
aagtcataaa acttttatgt atacttttcc attttatgaa atgtgcgaca ttaccatgta 29100
catgagcaaa cagtataagt tgtggccccc acaaaattgt gtggaaaaca ctggcacttt 29160
ctgctgcact gctatgctaa ttacagtgct cgctttggtc tgtaccctac tctatattaa 29220
atacaaaagc agacgcagct ttattgagga aaagaaaatg ccttaattta ctaagttaca 29280
aagctaatgt caccactaac tgctttactc gctgcttgca aaacaaattc aaaaagttag 29340
cattataatt agaataggat ttaaaccccc cggtcatttc ctgctcaata ccattcccct 29400
gaacaattga ctctatgtgg gatatgctcc agcgctacaa ccttgaagtc aggcttcctg 29460
gatgtcagca tctgactttg gccagcacct gtcccgcgga tttgttccag tccaactaca 29520
gcgacccacc ctaacagaga tgaccaacac aaccaacgcg gccgccgcta ccggacttac 29580
atctaccaca aatacacccc aagtttctgc ctttgtcaat aactgggata acttgggcat 29640
gtggtggttc tccatagcgc ttatgtttgt atgccttatt attatgtggc tcatctgctg 29700
cctaaagcgc aaacgcgccc gaccacccat ctatagtccc atcattgtgc tacacccaaa 29760
caatgatgga atccatagat tggacggact gaaacacatg ttcttttctc ttacagtatg 29820
attaaatgag acatgattcc tcgagttttt atattactga cccttgttgc gcttttttgt 29880
gcgtgctcca cattggctgc ggtttctcac atcgaagtag actgcattcc agccttcaca 29940
gtctatttgc tttacggatt tgtcaccctc acgctcatct gcagcctcat cactgtggtc 30000
atcgccttta tccagtgcat tgactgggtc tgtgtgcgct ttgcatatct cagacaccat 30060
ccccagtaca gggacaggac tatagctgag cttcttagaa ttctttaatt atgaaattta 30120
ctgtgacttt tctgctgatt atttgcaccc tatctgcgtt ttgttccccg acctccaagc 30180
ctcaaagaca tatatcatgc agattcactc gtatatggaa tattccaagt tgctacaatg 30240
aaaaaagcga tctttccgaa gcctggttat atgcaatcat ctctgttatg gtgttctgca 30300
gtaccatctt agccctagct atatatccct accttgacat tggctggaac gcaatagatg 30360
ccatgaacca cccaactttc cccgcgcccg ctatgcttcc actgcaacaa gttgttgccg 30420
gcggctttgt cccagccaat cagcctcgcc caccttctcc cacccccact gaaatcagct 30480
actttaatct aacaggagga gatgactgac accctagatc tagaaatgga cggaattatt 30540
acagagcagc gcctgctaga aagacgcagg gcagcggccg agcaacagcg catgaatcaa 30600
gagctccaag acatggttaa cttgcaccag tgcaaaaggg gtatcttttg tctggtaaag 30660
caggccaaag tcacctacga cagtaatacc accggacacc gccttagcta caagttgcca 30720
accaagcgtc agaaattggt ggtcatggtg ggagaaaagc ccattaccat aactcagcac 30780
tcggtagaaa ccgaaggctg cattcactca ccttgtcaag gacctgagga tctctgcacc 30840
cttattaaga ccctgtgcgg tctcaaagat cttattccct ttaactaata aaaaaaaata 30900
ataaagcatc acttacttaa aatcagttag caaatttctg tccagtttat tcagcagcac 30960
ctccttgccc tcctcccagc tctggtattg cagcttcctc ctggctgcaa actttctcca 31020
caatctaaat ggaatgtcag tttcctcctg ttcctgtcca tccgcaccca ctatcttcat 31080
gttgttgcag atgaagcgcg caagaccgtc tgaagatacc ttcaaccccg tgtatccata 31140
tgacacggaa accggtcctc caactgtgcc ttttcttact cctccctttg tatcccccaa 31200
tgggtttcaa gagagtcccc ctggggtact ctctttgcgc ctatccgaac ctctagttac 31260
ctccaatggc atgcttgcgc tcaaaatggg caacggcctc tctctggacg aggccggcaa 31320
ccttacctcc caaaatgtaa ccactgtgag cccacctctc aaaaaaacca agtcaaacat 31380
aaacctggaa atatctgcac ccctcacagt tacctcagaa gccctaactg tggctgccgc 31440
cgcacctcta atggtcgcgg gcaacacact caccatgcaa tcacaggccc cgctaaccgt 31500
gcacgactcc aaacttagca ttgccaccca aggacccctc acagtgtcag aaggaaagct 31560
agccctgcaa acatcaggcc ccctcaccac caccgatagc agtaccctta ctatcactgc 31620
ctcaccccct ctaactactg ccactggtag cttgggcatt gacttgaaag agcccattta 31680
tacacaaaat ggaaaactag gactaaagta cggggctcct ttgcatgtaa cagacgacct 31740
aaacactttg accgtagcaa ctggtccagg tgtgactatt aataatactt ccttgcaaac 31800
taaagttact ggagccttgg gttttgattc acaaggcaat atgcaactta atgtagcagg 31860
aggactaagg attgattctc aaaacagacg ccttatactt gatgttagtt atccgtttga 31920
tgctcaaaac caactaaatc taagactagg acagggccct ctttttataa actcagccca 31980
caacttggat attaactaca acaaaggcct ttacttgttt acagcttcaa acaattccaa 32040
aaagcttgag gttaacctaa gcactgccaa ggggttgatg tttgacgcta cagccatagc 32100
cattaatgca ggagatgggc ttgaatttgg ttcacctaat gcaccaaaca caaatcccct 32160
caaaacaaaa attggccatg gcctagaatt tgattcaaac aaggctatgg ttcctaaact 32220
aggaactggc cttagttttg acagcacagg tgccattaca gtaggaaaca aaaataatga 32280
taagctaact ttgtggacca caccagctcc atctcctaac tgtagactaa atgcagagaa 32340
agatgctaaa ctcactttgg tcttaacaaa atgtggcagt caaatacttg ctacagtttc 32400
agttttggct gttaaaggca gtttggctcc aatatctgga acagttcaaa gtgctcatct 32460
tattataaga tttgacgaaa atggagtgct actaaacaat tccttcctgg acccagaata 32520
ttggaacttt agaaatggag atcttactga aggcacagcc tatacaaacg ctgttggatt 32580
tatgcctaac ctatcagctt atccaaaatc tcacggtaaa actgccaaaa gtaacattgt 32640
cagtcaagtt tacttaaacg gagacaaaac taaacctgta acactaacca ttacactaaa 32700
cggtacacag gaaacaggag acacaactcc aagtgcatac tctatgtcat tttcatggga 32760
ctggtctggc cacaactaca ttaatgaaat atttgccaca tcctcttaca ctttttcata 32820
cattgcccaa gaaggaagcg gagctactaa cttcagcctg ctgaagcagg ctggtgacgt 32880
cgaggagaat cctggcccaa tggtgagcaa aggcgaagag ctgttcaccg gcgtggtgcc 32940
catcctggtg gagctggacg gcgacgtgaa cggccacaag ttcagcgtga gcggcgaggg 33000
cgagggcgac gccacctacg gcaagctgac cctgaagctg ctgtgcacca ccggcaagct 33060
gcccgtgccc tggcccaccc tggtgaccac cctgggctac ggcgtgcagt gcttcgcccg 33120
gtaccccgac cacatgaagc agcacgactt cttcaagagc gccatgcccg agggctacgt 33180
gcaggagcgg accatcttct tcaaggacga cggcaactac aagacccggg ccgaggtgaa 33240
gttcgagggc gacaccctgg tgaaccggat cgagctgaag ggcatcgact tcaaggagga 33300
cggcaacatc ctgggccaca agctggagta caactacaac agccacaacg tgtacatcac 33360
cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccggcaca acatcgagga 33420
cggcggcgtg cagctggccg accactacca gcagaacacc cccatcggcg acggccccgt 33480
gctgctgccc gacaaccact acctgagcta ccagagcgcc ctgttcaagg accccaacga 33540
gaagcgggac cacatggtgc tgctggagtt cctgaccgcc gccggcatca ccgagggcat 33600
gaacgagctc tataagtaat aaagaatcgt ttgtgttatg tttcaacgtg tttatttttc 33660
aattgcagaa aatttcgaat catttttcat tcagtagtat agccccacca ccacatagct 33720
tatacagatc accgtacctc aactttgtat aataaagttg taatcaaact cacagaaccc 33780
tagtattcaa cctgccacct ccctcccaac acacagagta cacagtcctt tctccccggc 33840
tggccttaaa aagcatcata tcatgggtaa cagacatatt cttaggtgtt atattccaca 33900
cggtttcctg tcgagccaaa cgctcatcag tgatattaat aaactccccg ggcagctcac 33960
ttaagttcat gtcgctgtcc agctgctgag ccacaggctg ctgtccaact tgcggttgct 34020
taacgggcgg cgaaggagaa gtccacgcct acatgggggt agagtcataa tcgtgcatca 34080
ggatagggcg gtggtgctgc agcagcgcgc gaataaactg ctgccgccgc cgctccgtcc 34140
tgcaggaata caacatggca gtggtctcct cagcgatgat tcgcaccgcc cgcagcataa 34200
ggcgccttgt cctccgggca cagcagcgca ccctgatctc acttaaatca gcacagtaac 34260
tgcagcacag caccacaata ttgttcaaaa tcccacagtg caaggcgctg tatccaaagc 34320
tcatggcggg gaccacagaa cccacgtggc catcatacca caagcgcagg tagattaagt 34380
ggcgacccct cataaacacg ctggacataa acattacctc ttttggcatg ttgtaattca 34440
ccacctcccg gtaccatata aacctctgat taaacatggc gccatccacc accatcctaa 34500
accagctggc caaaacctgc ccgccggcta tacactgcag ggaaccggga ctggaacaat 34560
gacagtggag agcccaggac tcgtaaccat ggatcatcat gctcgtcatg atatcaatgt 34620
tggcacaaca caggcacacg tgcatacact tcctcaggat tacaagctcc tcccgcgtta 34680
gaaccatatc ccagggaaca acccattcct gaatcagcgt aaatcccaca ctgcagggaa 34740
gacctcgcac gtaactcacg ttgtgcattg tcaaagtgtt acattcgggc agcagcggat 34800
gatcctccag tatggtagcg cgggtttctg tctcaaaagg aggtagacga tccctactgt 34860
acggagtgcg ccgagacaac cgagatcgtg ttggtcgtag tgtcatgcca aatggaacgc 34920
cggacgtagt catatttcct gaagcaaaac caggtgcggg cgtgacaaac agatctgcgt 34980
ctccggtctc gccgcttaga tcgctctgtg tagtagttgt agtatatcca ctctctcaaa 35040
gcatccaggc gccccctggc ttcgggttct atgtaaactc cttcatgcgc cgctgccctg 35100
ataacatcca ccaccgcaga ataagccaca cccagccaac ctacacattc gttctgcgag 35160
tcacacacgg gaggagcggg aagagctgga agaaccatgt tttttttttt attccaaaag 35220
attatccaaa acctcaaaat gaagatctat taagtgaacg cgctcccctc cggtggcgtg 35280
gtcaaactct acagccaaag aacagataat ggcatttgta agatgttgca caatggcttc 35340
caaaaggcaa acggccctca cgtccaagtg gacgtaaagg ctaaaccctt cagggtgaat 35400
ctcctctata aacattccag caccttcaac catgcccaaa taattctcat ctcgccacct 35460
tctcaatata tctctaagca aatcccgaat attaagtccg gccattgtaa aaatctgctc 35520
cagagcgccc tccaccttca gcctcaagca gcgaatcatg attgcaaaaa ttcaggttcc 35580
tcacagacct gtataagatt caaaagcgga acattaacaa aaataccgcg atcccgtagg 35640
tcccttcgca gggccagctg aacataatcg tgcaggtctg cacggaccag cgcggccact 35700
tccccgccag gaaccatgac aaaagaaccc acactgatta tgacacgcat actcggagct 35760
atgctaacca gcgtagcccc gatgtaagct tgttgcatgg gcggcgatat aaaatgcaag 35820
gtgctgctca aaaaatcagg caaagcctcg cgcaaaaaag aaagcacatc gtagtcatgc 35880
tcatgcagat aaaggcaggt aagctccgga accaccacag aaaaagacac catttttctc 35940
tcaaacatgt ctgcgggttt ctgcataaac acaaaataaa ataacaaaaa aacatttaaa 36000
cattagaagc ctgtcttaca acaggaaaaa caacccttat aagcataaga cggactacgg 36060
ccatgccggc gtgaccgtaa aaaaactggt caccgtgatt aaaaagcacc accgacagct 36120
cctcggtcat gtccggagtc ataatgtaag actcggtaaa cacatcaggt tgattcacat 36180
cggtcagtgc taaaaagcga ccgaaatagc ccgggggaat acatacccgc aggcgtagag 36240
acaacattac agcccccata ggaggtataa caaaattaat aggagagaaa aacacataaa 36300
cacctgaaaa accctcctgc ctaggcaaaa tagcaccctc ccgctccaga acaacataca 36360
gcgcttccac agcggcagcc ataacagtca gccttaccag taaaaaagaa aacctattaa 36420
aaaaacacca ctcgacacgg caccagctca atcagtcaca gtgtaaaaaa gggccaagtg 36480
cagagcgagt atatatagga ctaaaaaatg acgtaacggt taaagtccac aaaaaacacc 36540
cagaaaaccg cacgcgaacc tacgcccaga aacgaaagcc aaaaaaccca caacttcctc 36600
aaatcgtcac ttccgttttc ccacgttacg tcacttccca ttttaagaaa actacaattc 36660
ccaacacata caagttactc cgccctaaaa cctacgtcac ccgccccgtt cccacgcccc 36720
gcgccacgtc acaaactcca ccccctcatt atcatattgg cttcaatcca aaataaggta 36780
tattattgat gatg 36794
<210> 14
<211> 19
<212> PRT
<213> 人工序列
<220>
<223> 合成肽
<400> 14
Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn
1 5 10 15
Pro Gly Pro
<210> 15
<211> 22
<212> PRT
<213> 人工序列
<220>
<223> 合成肽
<400> 15
Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val
1 5 10 15
Glu Ser Asn Pro Gly Pro
20
<210> 16
<211> 20
<212> PRT
<213> 人工序列
<220>
<223> 合成肽
<400> 16
Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser
1 5 10 15
Asn Pro Gly Pro
20
<210> 17
<211> 18
<212> PRT
<213> 人工序列
<220>
<223> 合成肽
<400> 17
Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro
1 5 10 15
Gly Pro
<210> 18
<211> 22
<212> PRT
<213> 人工序列
<220>
<223> 合成肽
<400> 18
Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val
1 5 10 15
Glu Glu Asn Pro Gly Pro
20
<210> 19
<211> 25
<212> PRT
<213> 人工序列
<220>
<223> 合成肽
<400> 19
Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala
1 5 10 15
Gly Asp Val Glu Ser Asn Pro Gly Pro
20 25
<210> 20
<211> 23
<212> PRT
<213> 人工序列
<220>
<223> 合成肽
<400> 20
Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp
1 5 10 15
Val Glu Ser Asn Pro Gly Pro
20
<210> 21
<211> 21
<212> PRT
<213> 人工序列
<220>
<223> 合成肽
<400> 21
Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu
1 5 10 15
Glu Asn Pro Gly Pro
20
<210> 22
<211> 35876
<212> DNA
<213> 人工序列
<220>
<223> 合成腺病毒基因组Ad9 E3-15k-P2A-YPet
<400> 22
ctatctatat aatatacccc acaaagtaaa caaaagttaa tatgcaaatg agcttttgaa 60
ttttaacggt ttcggggcgg agccaacgct gattggacga gagaagacga tgcaaatgac 120
gtcacgacgc acggctaacg gtcgccgcgg aggcgtggcc tagcccggaa gcaagtcgcg 180
gggctgatga cgtataaaaa agcggacttt agacccggaa acggccgatt ttcccgcggc 240
cacgcccgga tatgaggtaa ttctgggcgg atgcaagtga aattaggtca ttttggcgcg 300
aaaactgaat gaggaagtga aaagcgaaaa ataccggtcc ctcccagggc ggaatattta 360
ccgagggccg agagactttg accgattacg tgggggtttc gattgcggtg tttttttcgc 420
gaatttccgc gtccgtgtca aagtccggtg tttatgtcac agatcagctg atccgcaggg 480
tatttaaacc agtcgagtcc gtcaagaggc cactcttgag tgccagcgag tagagatttc 540
tctgagctcc gctcccagag accgagaaaa atgagacacc tgcgcctcct gccttcaact 600
gtgcccggtg agctggctgt gcttatgctg gaggactttg tggatacagt attggaggac 660
gaactgcatc caagtccgtt cgagctggga cccacacttc aggatctcta tgatctggag 720
gtagatgccc atgatgacga ccctaacgag gaggctgtga atttaatatt tccagaatct 780
atgattcttc aggctgacat agccaacgaa tctactccac ttcatacacc gactctgtca 840
cccatacctg aattggaaga ggaggacgaa ctagacctcc ggtgttatga ggaaggtttt 900
cctcccagcg attcagagga tgaacggggt gagcagacca tggctctgat ctcagactat 960
gcttgtgtga ttgtggagga gcaagatgtg attgaaaaat ctactgagcc agtacaaggc 1020
tgtaggaact gccagtacca ccgggataag tccggagatg tgaacgcctc ctgcgctttg 1080
tgctatatga aacagacttt cagctttatt tacagtaagt ggagtgaatg tgagagaggc 1140
tgagtgctta acacataact gtaatgcttg aacagctgtg ctaagtgtgg tttattttgt 1200
tactaggtcc ggtgtcagag gatgagttat caccctcaga agaagaccac ccgtctcccc 1260
ctgagctgtc aggcgaaacg cccctgcaag tgttcagacc caccccagtc agacccagtg 1320
gcgagaggcg agcggctgtt gacaaaattg aggacttgtt gcaggacatg ggtggggatg 1380
aacctttgga cctgagcttg aaacgcccca ggaactagac gcagctgcgc ttagtcatgt 1440
gtaaataaag ttgtacaata aaagtctatg tgacgcatgc aaggtgtggt ttatgactca 1500
tgggcggggc ttagtcctat ataagtggca acacctgggc acttgggcac agaccttcag 1560
ggagttcctg atggatgtgt ggactatcct tgcagacttt agcaagacac gccggcttgt 1620
agaggatagt tcagacgggt gctccgggtt ctggagacac tggtttggaa ctcctctatc 1680
tcgcctggtg tacacagtta agaaggatta taaagaggaa tttgaaaata tttttgctga 1740
ctgctctggc ctgctagatt ctctgaatct tggccaccag tcccttttcc aggaaagggt 1800
actccacagc cttgattttt ccagcccagg gcgcactaca gccggggttg cttttgtggt 1860
ttttctggtt gacaaatgga gccaggacac ccaactgagc aggggctaca tcctggactt 1920
cgcagccatg cacctgtgga gggcctggat caggcagcgg ggacagagaa tcttgaatta 1980
ctggcttcta cagccagcag ctccgggtct tcttcgtcta cacagacaaa catccatgtt 2040
ggaggaagaa atgaggcagg ccatggacga gaacccgagg agcggcctgg accctccgtc 2100
ggaagaggag ctggattgaa tcaggtatcc agcctgtacc cagagcttag caaggtgctg 2160
acatccatgg ccaggggagt taagagggag aggagcgatg ggggtaatac cgggatgatg 2220
accgagctga cggccagcct gatgaatcgg aagcgcccag agcgccttac ctggtacgag 2280
ctacagcagg agtgcaggga tgagttgggc ctgatgcagg ataaatatgg cctggagcag 2340
ataaaaaccc attggttgaa cccagatgag gattgggagg aggctattaa gaagtatgcc 2400
aagatagccc tgcgcccaga ttgcaagtac atagtgacca agaccgtgaa tatcagacat 2460
gcctgctaca tctcggggaa cggggcagag gtggtcatcg ataccctgga caaggccgcc 2520
ttcaggtgtt gcatgatggg aatgagagca ggagtgatga atatgaattc catgatcttc 2580
atgaacatga agttcaatgg agagaagttt aatggggtgc tgttcatggc caacagccac 2640
atgaccctgc atggctgcag tttcttcggc ttcaacaata tgtgcgcaga ggtctggggc 2700
gcttccaaga tcaggggatg taagttttat ggctgctgga tgggcgtggt cggaagaccc 2760
aagagcgaga tgtctgtgaa gcagtgtgtg tttgagaaat gctacctggg agtctctacc 2820
gagggcaatg ctagagtgag acactgctct tccctggaga cgggctgctt ctgcctggtg 2880
aagggcacag cctctctgaa gcataatatg gtgaagggct gcacggatga gcgcatgtac 2940
aacatgctga cctgcgattc gggggtctgc catatcctga agaacatcca tgtgacctcc 3000
caccccagaa agaagtggcc agtgtttgag aataacctgc tgatcaagtg ccatatgcac 3060
ctgggagcca gaaggggcac cttccagccg taccagtgca actttagcca gaccaagctg 3120
ctgttggaga acgatgcctt ctccagggtg aacctgaacg gcatctttga catggatgtc 3180
tcggtgtaca agatcctgag atacgatgag accaagtcca gggtgcgcgc ttgcgagtgc 3240
gggggcagac acaccaggat gcagccagtg gccctggatg tgaccgagga gctgagacca 3300
gaccacctgg tgatggcctg taccgggacc gagttcagct ccagtgggga ggacacagat 3360
tagaggtagg tcgagtgagt agtgggcgtg gctaaggtga ctataaaggc gggtgtctta 3420
cgagggtctt tttgcttttc tgcagacatc atgaacggga ccggcggggc cttcgaaggg 3480
gggcttttta gcccttattt gacaacccgc ctgccgggat gggccggagt tcgtcagaat 3540
gtgatgggat cgacggtgga tgggcgccca gtgcttccag caaattcctc gaccatgacc 3600
tacgcgaccg tggggagctc gtcgctcgac agcaccgccg cagccgcggc agccgcagct 3660
gccatgacag cgacgagact ggcctcgagc tacatgccca gcagcggcag cagcccctct 3720
gtgcccagtt ccatcatcgc cgaggagaaa ctgctggccc tgctggccga gctggaagcc 3780
ctgagccgcc agctggccgc cctgacccag caggtgtccg agctccgcga gcagcaacag 3840
cagcaaaata aatgaaaact tcaataaaca cagattctga ttcaaacagc aaagcatctt 3900
tattatttat tttttcgcgc gcggtaggcc ctggtccacc tctcccgatc attgagagtg 3960
cggtggattt tttccaggac ccggtagagg tgggattgga tgttgaggta catgggcatg 4020
agcccgtccc gggggtggag gtagcaccac tgcatggcct cgtgctctgg ggtcgtgttg 4080
tagatgatcc agtcatagca ggggcgctgg gcgtggtgct ggatgatgtc tttgagaagg 4140
agactgatgg ccacggggag ccccttggtg taggtattgg cgaagcggtt gagctgggag 4200
ggatgcatgc ggggggagat gatgtgcagt ttggcctgga tcttgaggtt ggcgatgttg 4260
ccacccagat cccgccgggg gttcatgttg tgcaggacca ccaggacggt gtagcccgtg 4320
cacttgggga acttgtcatg caacttggaa gggaatgcgt ggaagaattt ggagacgccc 4380
ttgtgcccgc ccaggttttc catgcattca tccatgatga tggcgatggg cccgtgggct 4440
gcggctttgg caaagacgtt tctggggtca gatacatcat aattatgctc ctgggtgaga 4500
tcatcataag acattttaat gaatttgggg cggagggtgc cagattgggg gacgatggtt 4560
ccctcgggcc ccggggcgaa gttcccctca cagatctgca tctcccaggc tttcatctcg 4620
gaggggggga tcatgtccac ctgcggggcg atgaaaaaaa cggtttccgg ggctggggtg 4680
atgagctgcg aagagagcag gtttctcaac agctgggact tgccgcaccc ggtcgggccg 4740
tagatgaccc cgatgacggg ttgcaggtgg tagttcaagg acatgcagct gccgtcgtcc 4800
cggaggaggg gggccacctc gttgagcatg tctctgactt ggaggttttc ccgaacgagc 4860
tcgccgagga ggcggtcccc gcccagcgag aggagctctt gcagggaagc aaagtttttc 4920
aggggcttga gtccgtcggc catgggcatc ttggcgaggg tctgcgagag gagctccagg 4980
cggtcccaga gctcggtgac gtgctctacg gcatctcgat ccagcagact tcctcgtttc 5040
gggggttggg acgactgcga ctgtagggca cgagacgatg ggcgtccagc gcggccagcg 5100
tcatgtcctt ccagggtctc agggtccgag tgagggtggt ctccgtcacg gtgaaggggt 5160
gggcccctgg ctgggcgctt gcaagggtgc gcttgagact catcctgctg gtgctgaaac 5220
gggcacggtc ttcgccctgc gagtcggcga gatagcagtt gaccatgagc tcgtagttga 5280
gggcctcggc ggcgtggccc ttggcgcgga gcttgccctt ggaagagcgc ccgcaggcgg 5340
gacagaggag ggattgcagg gcgtagagct tgggggcgag aaagacggac tcgggagcga 5400
aagcgtccgc tccgcagtgg gcgcagacgg tctcgcactc gacgagccag gtgagctctg 5460
gctgctcggg gtcaaaaacc agttttcccc cgttcttttt gatgcgcttc ttacctcgcg 5520
tctccatgag tctgtgtccg cgctcggtga caaacaggct gtcggtgtcc ccgtagacgg 5580
acttgattgg cctgtcctgc aggggcgtcc cgcggtcctc ctcgtagaga aactcggacc 5640
actctgagac aaaggcgcgc gtccacgcca agacaaagga ggccacgtgc gaggggtagc 5700
ggtcgttgtc cactaggggg tccacctttt ccaccgtgtg cagacacatg tccccctcct 5760
ccgcatccaa gaaggtgatt ggcttgtagg tgtaggccac gtgaccgggg gtccccgacg 5820
ggggggtata aaagggggcg ggtctgtgct cgtcctcact ctcttccgcg tcgctgtcca 5880
cgagcgccag ctgttggggt aggtattccc tctcgagagc gggcatgacc tcggcactca 5940
ggttgtcagt ttctagaaac gaggaggatt tgatgttggc ttgccctgcc gcgatgcttt 6000
ttaggagact ttcatccatc tggtcagaaa agactatttt tttattgtca agcttggtgg 6060
cgaaggagcc atagagggcg ttggatagaa gcttggcgat ggatctcatg gtctgatttt 6120
tgtcacggtc ggcgcgctcc ttggccgcga tgtttagctg gacatactcg cgcgcgacgc 6180
acttccattc ggggaagacg gtggtgcgct cgtcgggcac gatcctgacg cgccagccgc 6240
ggttatgcag ggtgaccagg tccacgctgg tggccacctc gccgcgcagg ggctcgttgg 6300
tccagcagag tctgccgccc ttgcgcgagc agaacggggg cagcacatca agcagatgct 6360
cgtcaggggg gtccgcatcg atggtgaaga tgcccggaca gagttccttg tcaaaataat 6420
cgatttttga ggatgcatca tccaaggcca tctgccactc gcgggcggcc agcgctcgct 6480
cgtaggggtt gaggggcgga ccccagggca tgggatgagt gagggcggag gcgtacatgc 6540
cgcagatgtc gtagacatag atgggctccg agaggatgcc gatgtaggtg ggataacagc 6600
gccccccgcg gatgctggcg cgcacgtagt catacaactc gtgcgagggg gccaagaagg 6660
cggggccgag attggtgcgc tggggctgct cggcgcggaa gacgatctgg cgaaagatgg 6720
cgtgcgagtt ggaggagatg gtgggccgtt ggaagatgtt aaagtgggcg tggggcaagc 6780
ggaccgagtc gcggatgaag tgcgcgtagg agtcttgcag cttggcgacg agctcggcgg 6840
tgacgaggac gtccatggcg cagtagtcca gcgtttcgcg gatgatgtca taacccgcct 6900
ctcctttctt ctcccacagc tcgcggttga gggcgtactc ctcgtcatcc ttccagtact 6960
cccggagcgg gaatcctcga tcgtccgcac ggtaagagcc cagcatgtag aaatggttca 7020
cggccttgta gggacagcag cccttctcca cggggagggc gtaagcttga gcggccttgc 7080
ggagcgaggt gtgcgtcagg gcgaaggtgt ccctgaccat gactttcaag aactggtact 7140
tgaaatccga gtcgtcgcag ccgccgtgct cccagagctc gaaatcggtg cgcttcttcg 7200
agagggggtt aggcagagcg aaagtgacgt cattgaagag aatcttgcct gctcgcggca 7260
tgaaattgcg ggtgatgcgg aaagggcccg ggacggaggc tcggttgttg atcacctggg 7320
cggcaaggac gatctcgtcg aagccgttga tgttgtgccc gacgatgtag agttccatga 7380
atcgcgggcg gcctttgatg tgcggcagct ttttgagctc ctcgtaggtg aggtcctcgg 7440
ggcattgcag gccgtgctgc tctagcgccc actcctggag atgtgggttg gcttgcatga 7500
aggaagccca gagctcgcgg gccatgaggg tctggagctc gtcgcgaaag aggcggaact 7560
gctggcccac ggccatcttt tctggggtga cgcagtagaa ggtgaggggg tcccgctccc 7620
agcgatccca gcgtaagcgc acggcgagat cgcgagcgag ggcgaccagc tcgggttccc 7680
ccgagaattt catgaccagc atgaagggga cgagctgctt gccgaaggac cccatccagg 7740
tgtaggtttc tacatcgtag gtgacaaaga gccgctccgt gcgaggatga gagccgattg 7800
ggaagaactg gatttcctgc caccagttgg acgagtggct gttgatgtga tgaaagtaga 7860
aatcccgccg gcgaaccgag cactcgtgct gatgcttgta aaagcgtccg cagtactcgc 7920
agcgctgcac gggctgtacc tcatccacga gatacacagc gcgtcccttg aggaggaact 7980
tcaggagtgg cggccctggc tggtggcttt catgttcgcc tgcgtgggac tcaccctggg 8040
gctcctcgag gacggagagg ctgacgagcc cgcgcgggag ccaggtccag atctcggcgc 8100
ggcgggggcg gagagcgaag acgagggcgc gcagttggga gctgtccatg gtgtcgcgga 8160
gatccaggtc cgggggcagg gttctgaggt tgacctcgta gaggcgggtg agggcgtgct 8220
tgagatgcag atggtacttg atctccacgg gtgagttggt ggtcgtgtcc acgcattgca 8280
tgagcccgta gctgcgcggg gccacgaccg tgccgcggtg cgcttttaga agcggtgtcg 8340
cgggcgtgct cccggcggca gcggcggttc tggccccgcg ggcaggggcg gcagaggcac 8400
gtcggcgtgg cgctcgggca ggtcccggtg ctgcgccctg agagcgctgg cgtgcgcgac 8460
gacgcggcgg ttgacatcct ggatctgccg cctctgcgtg aagaccacgg gccccgtgac 8520
tttgaacctg aaagacagtt caacagaatc aatctctgcg tcattgacgg cggcctgacg 8580
caggatctct tgcacgtcgc ccgagttgtc ctggtaggcg atctcggtca tgaactgttc 8640
gatctcctcc tcctggagat cgccgcggcc cgcgcgctcc acggtggcgg cgaggtcatt 8700
ggagatgcgg cccatgagct gcgagaaggc gcccaggccg ctctcgttcc agacgcggct 8760
gtagaccacg tccccgtcgg cgtcgcgtgc gcgcatgacc acctgcgcga ggttgagctc 8820
cacgtgccgc gtgaagacgg cgtagttgcg caggcgctgg aagaggtagt tgagggtggt 8880
ggcgatgtgc tcggtgacga agaagtacat gatccagcgg cgcaggggca tctcgctgat 8940
gtcgccgatg gcctccagcc tttccatggc ctcgtagaaa tccacggcga agttgaaaaa 9000
ctgggcattg cgggccgaga ccgtgagctc gtcttccagg agccggatga gctcggcgat 9060
ggtggcgcgc acctcgcgct cgaaatcccc gggggcctcc tcttcttcct cttcttccat 9120
gacgacctct tcttctattt cttcctctgg gggcggtggt ggtggcgggg cccgacgacg 9180
acggcgacgc accgggagac ggtcgacgaa gcgctcgatc atctccccgc ggcggcgacg 9240
catggtttcg gtgacggcgc gaccccgttc gcgaggacgc agcgtgaaga cgccgccggt 9300
catctcccgg taatggggcg ggtccccgtt gggcagcgat agggcgctga cgatgcatct 9360
tatcaattgc ggtgtagggg acgtgagcgc gtcgagatcg accggatcgg agaatctttc 9420
gaggaaagcg tctagccaat cgcagtcgca aggtaagctc aaacacgtag cagccctgtg 9480
gacgctgtta gaattgcggt tgctgatgat gtaattgaag taggcgtttt tgaggcggcg 9540
gatggtggcg aggaggacca ggtccttggg tcccgcttgc tggatgcgga gccgctcggc 9600
catgccccag gcctggccct gacaccggct caggttcttg tagtagtcat gcatgagcct 9660
ctcgatgtca tcactggcgg aggcggagtc ttccatgcgg gtgaccccga cgcccctgag 9720
cggctgcacg agcgccaggt cggcgacgac gcgctcggcg aggatggcct gttgcacgcg 9780
ggtgagggtg tcctggaagt cgtccatgtc gacaaagcgg tggtaggccc cggtgttgat 9840
ggtgtaggtg cagttggcca tgagcgacca gttgacggtc tgcaggccgg gttgcacgac 9900
ctcggagtac ctgagccgcg agaaggcgcg cgagtcgaag acgtagtcat tgcaggtgcg 9960
cacgaggtac tggtagccga ctaggaagtg cggcggcggc tggcggtaga gcggccagcg 10020
ctgggtggcc ggcgcgcccg gggccaggtc ctcgagcatg aggcggtggt agccgtagag 10080
gtagcgggac atccaggtga tgccggcggc ggtggtggag gcgcgcggga actcgcggac 10140
gcggttccag atgttgcgca gcggcaggaa atagtccatg gtcggcacgg tctggccggt 10200
gagacgcgcg cagtcattga cgctctagag gcaaaaacga aagcggttga gcgggctctt 10260
cctccgtagc ctggcggaac gcaaacgggt taggccgcgt gtgtaccccg gttcgagtcc 10320
ccttgaatca ggctggagcc gcgactaacg tggtattggc actcccgtct cgacccgagc 10380
ccgatagccg ccaggatacg gcggagagtc ctttttgccg gccaaggggg gtcgctagac 10440
ttgaaagcga ccgaaaaccc tgccgggtag tggctcgcgc ccgtagtctg gagaagcatc 10500
gccagggttg agtcgcggca gaacccggtt cgcggacggc cgcggcgagc gggacttggt 10560
caccccgccg atttaaagac ccacagccag ccgacttctc cagttacggg agcgagcccc 10620
cttttttctt tttgccagat gcatcccgtc ctgcgccaaa tgcgtcccac ccccccggcg 10680
accaccgcga ccgcggccgt agcaggcgcc ggcgctagcc agccacagcc acagacagag 10740
atggacttgg aagagggcga agggctggcg agactggggg cgccgtcccc ggagcgacac 10800
ccccgcgtgc agctgcagaa ggacgtgcgc ccggcgtacg tgcctgcgca gaacctgttc 10860
agggaccgca gcggggagga gcccgaggag atgcgcgact gccggtttcg ggcgggcagg 10920
gagctgcgcg agggcctgga ccgccagcgc gtgctgcgcg acgaggattt cgagccgaac 10980
gagcagacgg ggatcagccc cgcgcgcgct cacgtggcgg cggccaacct ggtgacggcc 11040
tacgagcaga cggtgaagca ggagcgcaac ttccaaaaga gtttcaacaa ccacgtgcgc 11100
accctgatcg cgcgcgagga ggtggccctg ggcctgatgc acctgtggga cctggcggag 11160
gccatcgtgc agaacccgga cagcaagcct ctgacggcgc agctgttcct ggtggtgcag 11220
cacagcaggg acaacgaggc gttcagggag gcactgctga acatcgccga gcccgagggt 11280
cgctggctgc tggagctgat caacatcttg cagagcatcg tagtgcagga gcgcagcctg 11340
agcttggccg agaaggtggc ggcgatcaac tactcggtgc tgagcctggg caagttttac 11400
gcgcgcaaga tttacaagac gccgtacgtg cccatagaca aggaggtgaa gatagacagc 11460
ttttacatgc gcatggcgct caaggtgctg acgctgagcg acgacctggg cgtgtaccgc 11520
aacgaccgca tccacaaggc cgtgagcacg agccggcggc gcgagctaag cgaccgcgag 11580
ctgatgctga gcctgcgccg ggcgctggta gggggcgctg ccggcggcga ggagtcctac 11640
ttcgacatgg gggcggacct gcattggcag ccgagccggc gcgccttgga ggccgcctac 11700
ggtccagagg acttggatga ggaagaggaa gaggaggagg atgcacccgc tgcggggtac 11760
tgacgcctcc gtgatgtgtt tttagatgca gcaagccccg gaccccgcca taagggcggc 11820
gctgcaaagc cagccgtccg gtctagcatc ggacgactgg gaggccgcga tgcaacgcat 11880
catggccctg acgacccgca accccgagtc ctttagacaa cagccgcagg ccaacagact 11940
ctcggccatt ctggaggcgg tggtcccctc tcggaccaac cccacgcacg agaaggtgct 12000
ggcgatcgtg aacgcgctgg cggagaacaa ggccatccgt cccgacgagg ccgggctggt 12060
gtacaacgcc ctgctggagc gcgtgggccg ctacaacagc acgaacgtgc agtccaacct 12120
ggaccggctg gtgacggacg tgcgcgaggc cgtggcgcag cgcgagcggt tcaagaacga 12180
gggcctgggc tcgctggtgg cgctgaacgc cttcctggcg acgcagccgg cgaacgtgcc 12240
gcgcgggcag gacgattaca ccaactttat cagcgcgctg cggctgatgg tgaccgaggt 12300
tccccagagc gaggtgtacc agtcgggccc ggactacttt ttccagacga gccggcaggg 12360
cttgcagacg gtgaacctga gccaggcttt caagaacctg cgagggctgt ggggcgtgca 12420
ggcgcccgtg ggcgaccggt cgacggtgag cagcttgctg acgcccaact cgcggctgct 12480
gctgctgctg atcgcgccct tcaccgacag cggcagcgtg aaccgcaact cgtacctggg 12540
ccatctgctg acgctgtacc gcgaggccat aggccaggcg caggtggacg agcagacctt 12600
ccaggagatc actagcgtga gccgcgcgct ggggcagaac gacaccgaca gtctgagggc 12660
caccctgaac ttcttgctga ccaatagaca gcagaagatc ccggcgcaat atgcgctgtc 12720
ggccgaggag gaaaggatcc tgagatatgt gcagcagagc gtagggctgt tcctgatgca 12780
ggagggggcc acccccagcg ccgcgctgga catgaccgcg cgcaacatgg aacctagcat 12840
gtacgccgcc aaccggccgt tcatcaataa gctgatggac tacctgcacc gcgcggcggt 12900
catgaacacg gactacttta caaacgccat cctgaacccg cactggctcc cgccgccggg 12960
gttctacacg ggcgagtacg acatgcccga ccccaacgac gggttcctgt gggacgacgt 13020
ggacagcgtg gtgttctcgc cgacctttca aaagcgccag gaggcgccgc cgagcgaggg 13080
cgcggtgggg aggagcccct ttcctagctt agggagtttg catagcttgc cgggctcggt 13140
gaacagcggc agggtgagcc ggccgcgctt gctgggcgag gacgagtacc tgaacgactc 13200
gctgctgcag ccgccgcggg ccaagaacgc catggccaat aacgggatag agagtctggt 13260
ggacaaactg aaccgttgga agacctacgc tcaggaccat agggatgcgc ccgcgccgcg 13320
gcgacagcgc cacgaccggc agcggggcct ggtgtgggac gacgaggact cggccgacga 13380
tagcagcgtg ttggacttgg gcgggagcgg tggggtcaac ccgttcgcgc atctgcagcc 13440
caaactgggg cgacggatgt tttgaaatgc aaaataaaac tcaccaaggc catagcgtgc 13500
gttctcttcc ttgttagaga tgaggcgtgc ggtggtgtct tcctctcctc ctccctcgta 13560
cgagagcgtg atggcgcagg cgaccctgga ggttccgttt gtgcctccgc ggtatatggc 13620
tcctacggag ggcagaaaca gcattcgtta ctcggagctg gctccgcagt acgacaccac 13680
tcgcgtgtac ttggtggaca acaagtcggc ggacatcgct tccctgaact accaaaacga 13740
ccacagcaac ttcctgacca cggtggtgca gaacaacgat ttcacccccg ccgaggccag 13800
cacgcagacg ataaattttg acgagcggtc gcggtggggc ggtgatctga agaccattct 13860
gcacactaac atgcccaatg tgaacgagta catgttcacc agcaagttta aggcgcgggt 13920
gatggtggct aggaagcatc cagagggggt agttgaaaca gatttgagtc aggataagct 13980
tgaatatgag tggtttgagt ttaccctgcc cgagggaaac ttttccgaga ccatgaccat 14040
agacctgatg aacaacgcca tcttggaaaa ctacttgcaa gtggggcggc aaaatggcgt 14100
gctggagagc gatatcggag tcaagtttga cagcagaaat ttcaagctgg gctgggaccc 14160
ggtgaccaag ctggtgatgc caggggtcta cacctacgag gccttccacc cggacgtggt 14220
gctgctgccg ggctgcgggg tggacttcac cgagagccgc ctgagcaacc tcctgggcat 14280
tcgcaagaag caacctttcc aagagggctt cagaatcatg tatgaggatc tagaaggtgg 14340
caacatcccc gccctccttg atgtgcccaa gtacttggaa agcaagaaga aagttgaaga 14400
cgaaactaaa aatgcagctg cggctacagc cgatacaacc actaggggtg atacatttgc 14460
aactccagcg caagagacag cagctgataa gaaggtagaa gtcttgccca ttgaaaagga 14520
tgagagtggt agaagttaca acctgatcca ggggacccac gacacgctgt accgcagttg 14580
gtacctgtcc tatacctacg gggaccccga gaagggggtg cagtcgtgga cgctgctcac 14640
caccccggac gtcacctgcg gcgcggagca agtctactgg tcgctgccgg acctcatgca 14700
agacccagtc accttccgct ctacccagca agtcagcaac taccccgtgg tcggcgccga 14760
gctcatgccc ttccgcgcca agagctttta caacgacctc gccgtctact cccagctcat 14820
ccgcagctac acctccctca cccacgtctt caaccgcttc cccgacaacc agatcctctg 14880
ccgcccgccc gcgcccacca tcaccaccgt cagtgaaaac gtgcctgctc tcacagatca 14940
cgggacgcta ccgctgcgca gcagtatccg cggagtccag cgagtgaccg tcactgacgc 15000
ccgtcgccgc acctgtccct acgtctacaa ggccctgggc atagtcgcgc cgcgcgtgct 15060
ttccagtcgc accttctaaa aaaatgtcta ttctcatctc gcccagcaat aacaccggct 15120
ggggtcttac tagacccagc accatgtacg gaggagccaa gaagcgctcc cagcagcacc 15180
ccgtccgcgt ccgcggccac ttccgcgctc cctggggcgc ttacaagcgc gggcggactt 15240
ccaccgccgt gcgcaccacc gtcgacgacg tcatcgactc ggtggtcgcc gacgcgcgca 15300
actatacccc cgccccctcc accgtggacg cggtcatcga cagcgtggtg gccgacgcgc 15360
gcgactatgc cagacgcaag agccggcggc gacggatcgc caggcgccac cggagcacgc 15420
ccgccatgcg cgccgcccgg gctctgctgc gccgcgccag acgcacgggc cgccgggcca 15480
tgatgcgagc cgcgcgccgc gctgccactg cacccacccc cgcaggcagg actcgcagac 15540
gagcggccgc cgccgccgcc gcggccatct ctagcatgac cagacccagg cgcggaaacg 15600
tgtactgggt gcgcgactcc gtcacgggcg tgcgcgtgcc cgtgcgcacc cgtcctcctc 15660
gtccctgatc taatgcttgt gtcctccccc gcaagcgacg atgtcaaagc gcaaaatcaa 15720
ggaggagatg ctccaggtcg tcgccccgga gatttacgga ccaccccagg cggaccagaa 15780
accccgcaaa atcaagcggg ttaaaaaaaa ggatgaggtg gacgaggggg cagtagagtt 15840
tgtgcgcgag ttcgctccgc ggcggcgcgt aaattggaag gggcgcaggg tgcagcgcgt 15900
gttgcggccc ggcacggcgg tggtgttcac gcccggcgag cggtcctcag tcaggagcaa 15960
gcgtagctat gacgaggtgt acggcgacga cgacatcctg gaccaggcgg cggagcgggc 16020
gggcgagttc gcctacggga agcggtcgcg cgaagaggag ctgatctcgc tgccgctgga 16080
cgaaagcaac cccacgccga gcctgaagcc cgtgaccctg cagcaggtgc tgccccaggc 16140
ggtgctgctg ccgagccgcg gggtcaagcg cgagggcgag agcatgtacc cgaccatgca 16200
gatcatggtg cccaagcgcc ggcgcgtgga ggacgtgctg gacaccgtga aaatggatgt 16260
ggagcccgag gtcaaggtgc gccccatcaa gcaggtggcg ccgggcctgg gcgtgcagac 16320
cgtggacatt cagatcccca ccgacatgga tgtcgacaaa aaaccctcga ccagcatcga 16380
ggtgcagacc gacccctggc tcccagcctc cactgctacc gtctccactt ctaccgccgc 16440
cacggctacc gagcctccca ggaggcgaag atggggcgcc gccagccggc tgatgcccaa 16500
ctacgtggtg catccttcca tcatcccgac gccgggctac cgcggcaccc ggtactacgc 16560
cagccgccgg cgcccagcca gcaaacgccg ccgccgcacc gccacccgcc gccgtctggc 16620
ccccgcccgc gtgcgccgcg tgaccacgcg ccggggccgc tcgctcgttc tgcccaccgt 16680
gcgctaccac cccagcatcc tttaattcgt gtgctgtgat actgttgcag agagatggct 16740
ctcacttgcc gcctgcgcat ccccgtcccg aattaccgag gaagatcccg ccgcaggaga 16800
ggcatggcag gcagcggcct gaaccgccgc cggcggcggg ccatgcgcag gcgcctgagt 16860
ggcggctttc tgcccgcgct catccccata atcgccgcgg ccatcggcac gatcccgggc 16920
atagcttccg ttgcgctgca ggcgtcgcag cgccgttgat gtgcgaataa aagcctcttt 16980
agactctgac acacctggtc ctgtatattt ttagaatgga agacatcaat tttgcgtccc 17040
tggctccgcg gcacggcacg cggccgttca tgggcacctg gaacgagatc ggcaccagcc 17100
agctgaacgg gggcgccttc aattggagca gtgtctggag cgggcttaaa aatttcggct 17160
cgacgctccg gacctatggg aacaaggcct ggaatagtag cacggggcag ttgctaaggg 17220
aaaagctcaa agaccagaac tttcagcaga aggtggtgga cgggctggcc tcgggcatta 17280
acggggtggt ggacatcgcg aaccaggccg tgcagcgcga gataaacagc cgcctggacc 17340
cgcggccgcc cacggtggtg gagatggaag atgcaactct tccgccgccc aaaggcgaga 17400
agcggccgcg gcccgacgcg gaggagacga tcctgcaggt ggacgagccg ccctcgtacg 17460
aggaggccgt caaggccggc atgcccacca cgcgcatcat cgcgccgctg gccacgggtg 17520
taatgaaacc cgccaccctt gacctgcctc caccacccgc gcccgctcca ccgaaggcag 17580
ctcccgcggt cgtgcaggcc cccccggtgg cgaccgccgt gcgccgcgtc cccgcccgcc 17640
gccaggccca gaactggcag agcacgttgc acagtatcgt aggcctggga gtgaaaagtc 17700
tgaagcgccg ccgatgctat tgaaagagag gaaagaggac actaaaggga gagcttaact 17760
tgtatgtgcc ttaccgccag agaacgcgcg aagatggcca ccccctcgat gatgccgcag 17820
tgggcgtaca tgcacatcgc cgggcaggac gcctcggagt acctgagccc gggtctggtg 17880
cagtttgccc gcgccaccga cacgtacttc agcctgggca acaagtttag gaaccccacg 17940
gtggccccga cccacgatgt gaccacggac cggtcccagc gtctgacgct gcgcttcgtg 18000
cccgtggatc gcgaggacac cacgtactcg tacaaggcgc gcttcactct ggccgtgggc 18060
gacaaccggg tgctagacat ggccagcact tactttgaca tccgcggcgt cctggaccgc 18120
ggtcccagct tcaaacccta ctcgggcaca gcttacaaca gtctggcccc caagggtgcc 18180
cccaactcca gccagtggct tgcaaaagac accaatgctg gcgatcaagc attaaaaacc 18240
cacacacatg gcgtagctgc tatgggggga acagatatca cagcaaaggg tttgcaaatt 18300
ggtgttgaca cgactgaaaa caagaatgag cctatttatg caaatgaaat ataccagcca 18360
gaacctcagg taggagagga aaacttgcaa gatgttgaaa acttttatgg aggcagagct 18420
cttaaaaaag aaaccaaaat gaaaccttgc tatggctcgt ttgccagacc cacaaatgaa 18480
aaaggcggtc aagccaaatt tttaactgac ggcgatggtc agctaactaa aaatcatgat 18540
atcacaatga atttctttga cactcctgga ggaacagttg gtcaggatac tgaacttgaa 18600
gcagacattg ttatgtatgc tgagaatgtg catctggaaa ctccagacac gcatgtggtg 18660
tacaaaccag gaacttctga tgagagttca gaagcaaatt tggttcagca gtccatgcca 18720
aacaggccca actacatcgg cttcagggac aactttgtgg gtctcatgta ctataacagc 18780
actggcaaca tgggtgtgct ggctggtcaa gcatctcagt tgaatgctgt ggtcgacttg 18840
caagacagaa acacagagct gtcttaccag ctcttgctag attctctggg tgacagaacc 18900
agatacttta gcatgtggaa ctctgcagtg gacagttatg atcctgatgt caggattatt 18960
gaaaatcacg gtgtggaaga tgaacttcca aactattgct tcccattgga tggagctggc 19020
actaatgcta cctaccaagg tgtaaaagtt aaaaatggcc aagatggaga tgtaaacgca 19080
gattgggaaa aagatccaaa tcttgcttca cgaaaccaaa tatgcaaggg taacatcttc 19140
gccatggaga tcaacctcca ggccaacctg tggaagagtt ttctgtactc gaatgtggcc 19200
ctgtacctgc ccgactcata caagtacacg ccggccaacg tcacgctgcc cgccaatacc 19260
aacacctacg agtacatgaa cggccgcgtg gtagccccct cgctggtgga cgcctacatc 19320
aacatcggcg cccggtggtc gctggacccc atggacaacg tcaacccatt caaccaccac 19380
cgcaacgcgg gcctgcgtta ccgctccatg cttctgggca acggccgcta cgtgcccttc 19440
cacatccaag tgccccaaaa gttctttgcc atcaagaacc tgctcctgct ccccggctcc 19500
tacacctacg agtggaactt ccgcaaggat gtcaacatga tcctgcagag ttccctcgga 19560
aacgacctgc gcgtcgacgg cgcctccgtc cgcttcgaca gcgtcaacct ctacgccaca 19620
ttcttcccca tggcgcacaa caccgcctcc accctggaag ccatgctgcg caacgacacc 19680
aacgaccagt ccttcaacga ctacctctcg gccgccaaca tgctctaccc catcccggcc 19740
aaggccacca acgtgcccat ctccatcccc tcgcgcaact gggccgcctt ccgcggctgg 19800
agtttcaccc ggctcaagac caaagaaact ccctccctcg gctcgggttt cgatccctac 19860
tttgtatact cgggttccat cccctacctc gacgggacct tctacctcaa ccacaccttc 19920
aagaaggtct ccatcatgtt cgactcctcg gtcagctggc ccggcaacga ccggctgctc 19980
acgccgaacg agttcgagat caagcgcagt gtcgacgggg agggctacaa tgtggcccaa 20040
tgcaacatga ccaaggactg gttcctcgtc cagatgctct cccactacaa catcggctac 20100
cagggcttcc acgtgcccga gggctacaag gaccgcatgt actccttctt ccgcaacttc 20160
cagcccatga gcaggcaggt ggtcgatgag atcaactaca aggactacaa ggccgtcacc 20220
ctgcccttcc agcacaacaa ctcgggcttc accggctacc ttgcacccac catgcgtcag 20280
gggcagccct accccgccaa cttcccctat cctctcatcg gccagacagc cgtgccctct 20340
gtcacccaga aaaagttcct ctgcgacagg gtcatgtggc gcatcccctt ctccagcaac 20400
ttcatgtcca tgggcgccct caccgacctg ggtcagaaca tgctctatgc caactcggcc 20460
cacgcgctcg acatgacctt cgaggtggac cccatggatg agcccaccct cctctatctt 20520
ctcttcgaag ttttcgacgt ggtcagagtg caccagccgc accgcggcgt catcgaggcc 20580
gtctacctgc gcacgccctt ctccgccggc aacgccacca cctaagcatg agcggctcca 20640
gcgaacgaga gctcgcggcc atcgtgcgcg acctgggctg cgggccctac tttttgggca 20700
cccacgacaa gcgcttcccg ggcttcctcg ccggcgacaa gctggcctgc gccattgtca 20760
acacggccgg ccgcgagacc ggaggcgtgc actggctcgc cttcggctgg aacccgcgct 20820
cgcgcacctg ctacatgttc gacccatttg ggttctcgga ccgccggctc aagcagattt 20880
acagcttcga gtacgaggct atgttgcgcc gaagcgccct ggcttcctcg cccgaccgct 20940
gtctcagcct cgagcagtcc acccagaccg tgcaggggcc cgactccgcc gcctgcggac 21000
ttttctgttg catgttcttg catgccttcg tgcactggcc cgaccgaccc atggacggga 21060
accccaccat gaacttgctg acgggggtgc ccaacggcat gctacaatcg ccacaggtgc 21120
tgcccaccct ccggcgcaac caggaggagc tctaccgctt cctcgcgcgc cactcccctt 21180
actttcgctc ccaccgcgcc gccatcgaac acgccaccgc ttttgacaaa atgaaacaac 21240
tgcgtgtatc tcaataaaca gcacttttat tttacatgta ctggagtata tgcaagttat 21300
ttaaaagtcg aaggggttct cgcgctcgtc gttgtgcgcc gcgctgggga gggccacgtt 21360
gcggtactgg aacttgggct gccacttgaa ctcggggatc accagtttgg gcactggggt 21420
ctcggggaag gtctcgctcc acatgcgccg gctcatctgc agggcgccca gtatgtccgg 21480
ggcggagatc ttgaaatcgc agttggggcc ggtgctctgc gcgcgcgagt tgcggtacac 21540
ggggttgcag cactggaaca ccatcagact ggggtacttc acactggcca gcacgctctt 21600
gtcgctgatc tgatccttgt ccaggtcctc ggcgttgctc aggccgaacg gggtcatctt 21660
gcacagctgg cggcccagga agggcacgct ctgaggcttg tggttacact cgcagtgcac 21720
gggcatcagc atcatccccg cgccgcgctg catattcggg tagagggcct tgacaaaggc 21780
cgagatctgc ttgaaagctt gctgggcctt ggccccctcg ctgaaaaaca ggccgcagct 21840
cttcccgctg aactggttat tcccgcaccc ggcatcatgc acgcagcagc gcgcgtcatg 21900
gctggtcagt tgcaccacgc tccgtcccca gcggttctgg gtcaccttgg ccttgctagg 21960
ctgctccttc aacgcgcgct ggccgttctc gctggtcaca tccatctcca ccacgtggtc 22020
cttgtggatc atcaccgtcc cgtgcaaaca cttgagctgg ccttccacct cggtgcagcc 22080
gtgatcccac agggcgcagc cggtgcactc ccaattcttg tgtgcgatcc cgctgtggct 22140
gaagatgtaa ccttgcaaca ggcgacccat gacggtgcta aatgatttac tggtgctgaa 22200
ggtcagttgc agaccgcggg cctcctcgtt catccaggtc tggcacatct tttggaagat 22260
ctcggtctgc tcgggcatga gcttgtaagc atcgcgcagg ccgctgtcga cgcggtagcg 22320
ttccataagc acgttcatgg tatccatgcc cttctcccag gacgagacca gaggcagact 22380
cagggggttg cgcacgttca gaataccggg ggtcgcgggc tcgacgatgc gttttccgtc 22440
cttgccttcc ttcaacagaa ccggcggctg gctgaatccc actcccacga tcacggcatc 22500
ttcctggggc atctcttcgt cggggtctac cttggtcaca tgcttggtct ttctggcttg 22560
cttctttttt ggagggctgt ccacggggac cacgtcctcc tcggaagacc cggagcccac 22620
ccgctgatac tttcggcgct tggtgggcag aggaggtgga ggcgaggggc tcctctcctg 22680
ctccggcgga tagcgcgccg acccgtggcc ccggggcgga gtggcctctc gctccatgaa 22740
ccggcgcacg tcctgactgc cgccggccat tgtttcctag gggaagatgg aggagcagcc 22800
gcgtaagcag gagcaggagg aggacttaac cacccacgag caacccaaaa tcgagcagga 22860
cctgggcttc gaagagccgg ctcgtctaga acccccacag gatgaacagg agcacgagca 22920
agacgcaggc caggaggaga ccgacgctgg gctcgagcat ggctacctgg gaggagagga 22980
ggatgtgctg ctgaaacact tgcagcgcca gtccctcatc ctccgggacg ccctggccga 23040
ccggagcgaa acccccctca gcgtcgagga gctgtgtcgg gcctacgagc tcaacctctt 23100
ctcgccgcgc gtgcccccca aacgccagcc caacggcacc tgcgagccca acccgcgtct 23160
caacttctat cccgttttcg cggtccccga ggcccttgcc acctatcaca tttttttcaa 23220
gaaccaaaag atccccgtct cctgccgcgc caaccgcacc cgcgccgacg cgctcctcgc 23280
tctggggccc ggcgcgcgca tacctgatat cgcttccctg gaagaggtgc ccaagatctt 23340
cgaagggctc ggtcgggacg agacgcgcgc ggcgaacgct ctgaaagaaa cagcagagga 23400
agagggtcac actagcgccc tggtagagtt ggaaggcgac aacgccaggc tggccgtgct 23460
caagcgcagc gtcgagctca cccactttgc ctaccccgcc gtcaacctcc cgcccaaggt 23520
catgcgtcac atcatggatc agctcatcat gccccacatc gaagccctcg atgaaagtca 23580
ggagcagcgc cccgaggacg cccgacccgt ggtcagcgac gagatgctcg cgcgctggct 23640
cgggacccgc gagccccagg ctttggaaca gcggcgcaag ctcatgctgg ccgtggtcct 23700
ggtcaccctc gagctcgaat gcatgcgccg cttcttcagc gaccccgaga ccctgcgcaa 23760
agtcgaggag accctgcact acactttcag acacggtttc gtcaggcagg cctgcaagat 23820
ttccaacgtg gagctgacca acctggtctc ctgcctgggg atcctgcatg agaaccgcct 23880
gggacagacc gtactccact ctaccctgaa gggcgaggcg cggagggact atgtccgcga 23940
ctgcgtcttt ctctttctct gccacacatg gcaagcagcc atgggcgtgt ggcagcagtg 24000
tctcgaggac gagaacctga aggagctgga caagcttctt gctagaaacc ttaaaaagct 24060
ttggacgggc ttcgacgagc gcaccgtcgc ctcggacctg gccgagatcg tcttccccga 24120
gcgcctgagg cagacgctga aaggcgggct gcccgacttc atgagccaga gcatgttgca 24180
aaactaccgc actttcattc tcgagcgatc ggggatgctg cccgccacct gcaacgcttt 24240
cccctccgac tttgtcccgc tgagctaccg cgagtgtccc ccgccgctgt ggagccactg 24300
ctacctcttg cagctggcca actacatcgc ctaccactcg gacgtgatcg aggacgtgag 24360
cggcgagggg ctgctcgagt gccactgccg ctgcaacctg tgctccccgc accgctccct 24420
ggtctgcaac ccccagctcc ttagcgagac ccaggtcatc ggtaccttcg agctgcaagg 24480
tccgcaggag tccaccgctc cgctgaaact cacgccgggg ttgtggactt ccgcgtacct 24540
gcgcaaattt gtacccgagg actaccacgc ccatgagata aagttcttcg aggaccaatc 24600
gcggccgcag cacgcggatc tcacggcctg cgtcatcacc cagggcgcga tcctcgccca 24660
attgcacgcc atccaaaaat cccgccaaga gtttcttctg aaaaagggta gaggggtcta 24720
cctggacccc cagacgggcg aggtgctcaa cacgggtctc ccccagcatg ccgaggaaga 24780
agcaggagcc gctagtggag gagatggaag aagaatggga cagccaggca gaggaggacg 24840
aatgggagga ggagacagag gaggaagaat tggaagaggt ggaagaggag caggcaacag 24900
agcagcccgt cgccgcacca tccgcgccgg cagccccggc ggtcacggat acaacctccg 24960
cagctccggc caagcctcct cgtagatggg atcgagtgaa gggcgacggt aagcacgagc 25020
ggcagggcta ccgatcatgg agggcccaca aagccgcgat catcgcctgc ttgcaagact 25080
gcggggggaa catcgctttc gcccgccgct acctgctctt ccaccgcggg gtgaacatcc 25140
cccgcaacgt gttgcattac taccgtcacc ttcacagcta agaaaaagca agtaagagaa 25200
gtcgccggag gaggcctgag gatcgcggcg aacgagccct tgaccaccag ggagctgagg 25260
aaccggatct tccccactct ttatgccatt tttcagcaga gtcgaggtca gcagcaagag 25320
ctcaaagtaa aaaaccggtc tctgcgctcg ctcacccgca gttgcttgta ccacaaaaac 25380
gaagatcagc tgcagcgcac tctcgaagac gccgaggctc tgttccacaa gtactgcgcg 25440
ctcactctta aagactaagg cgcgcccacc cggaaaaaag gcgggaatta cctcatcgcc 25500
accttaatga gcaaggagat tcccacccct tacatgtgga gctatcagcc ccagatgggc 25560
ctggccgcgg gcgcctccca agactactcc acacgcatga actggctcag tgccggcccc 25620
tcgatgatat cacgggtcaa cggggtccgc agtcatcgaa accagatatt gttggagcag 25680
gcggcggtca cctccacgcc cagggcaaag ctcaacccgc gtaattggcc ctccaccctg 25740
gtgtatcagg aaatccccgg gccgactacc gtactacttc cgcgtgacgc actggccgaa 25800
gtccgcatga ctaactcagg tgtccagctg gccggcggcg cttcccggtg cccgctccgc 25860
ccacaatcgg gtataaaaac cctggtgatc cgaggcagag gcacacagct caacgacgag 25920
ttggtgagct cttcgatcgg tctgcgaccg gacggagtgt tccaactagc cggagccggg 25980
agatcgtcct tcactcccaa ccaggcctac ctgaccttgc agagcagctc ttcggagcct 26040
cgctcgggag gcatcggaac cctccagttc gtggaggagt ttgtgccctc ggtctacttc 26100
aaccccttct cgggatcgcc aggcctctac ccggacgagt tcataccgaa cttcgacgca 26160
gtgagagaag cggtggacgg ctacgactga atgtcccatg gtgactctgc tgagctcgct 26220
cggttgaggc atctggacca ctgccgccgc ctgcgctgct tcgcccggga gagctgcgga 26280
ctcatctact ttgagtttcc cgaggagcac cccaacggtc ctgcacacgg agtgcggatc 26340
accgtagagg gcaccaccga gtctcacctg gtcaggttct tcacccagca acccttcctg 26400
gtcgagcggg accggggcgc caccacctac accgtctact gcatctgtcc gaccccgaag 26460
ttgcatgaga atttttgctg tactctttgt ggtgagttta ataaaagcta aactcttgca 26520
atactctgga ccttgtcgtc atcaactcaa cgagaccgtc tacctcacca accagactga 26580
ggtaaaactc acctgcagac cacacaagac ctatatcatc tggttcttcg agaacacctc 26640
atttgcagtc tcaaacactc actgcaacga cggtgttgaa cttccaaaca acctttccag 26700
tggactgagt tacgatacac atagagctaa gctcgtcctc tacaatcctt tcgtagaggg 26760
aacctaccac tgccagagcg gaccttgctt ccacagtttt actttggtga acgttaccgg 26820
cagcagcaca gccgctccag aacctaacct tccttctgat actatcaaac ctcgtttcgg 26880
aggtgagcta aggcttcccc cttctgagga gggggttagc ccatacgaag tggtcgggta 26940
tttgatttta ggggtggtcc tgggtgggtg catagcggtg ctagctcagc tgccttgctg 27000
ggtagaaatc aaaatcttta tatgctgggt cagacattgt ggggaggaac tatgaagggg 27060
ctcttgctga ttatcctttc cctggtgggg ggtgtgctgt catgccacga acagccacga 27120
tgtaacatca ccacaggcaa tgagaggagt gtcatatgca cagtagtcat caaatgcgag 27180
catgaatgcc ctctcaacat cacattcaaa aaccgtacca tggggaatgc atgggtgggc 27240
gactgggaac caggagatga gcagaactac acggtcactg tccatggtag cgatggaaat 27300
cacactttcg gtttcaaatt catttttgaa gtcatgtgtg atatcacact gcatgtggct 27360
agacttcatg gcttgtggcc ccctaccaag gaaaacatgg ttgggttttc tttggctttt 27420
gtgatcatgg cctgctttat gtcaggtctg ctggtaggag ctctagtgtg gttcctgaaa 27480
cgcaagccca ggtatggaaa tgaggagaag gaaaaattgc tataaattct ttttcttttc 27540
acagcaccat gaatactttg accagtgtcg tgctgctctc tcttcttgta gcttttagtc 27600
aggcaggaat tattaactta aatgtatcat ggggaatgaa tctaacttta gtgggaccct 27660
cagacctgcc agttacatgg tatgatggaa agggaatgca gttttgtgat ggaaatacaa 27720
ttaagaaccc acaaatcaag catagctgta atcaacagaa tctaacttta cttaatgctg 27780
acaagtctca tgaaagaact tacctaggtt acagacatga cagtaaggga aaagtagact 27840
ataaggttac agtcatacca cctcctccag ccactcgcaa gcctttgtca gagcctcatt 27900
atgttactgt gactatgggc gataacataa ctttagtggg accctcagac ctgccagtta 27960
catggtatga tggagaagga aataaattct gcgatggaga aaaagttgaa catgcagaat 28020
ttaatcatac atgtaacatc cagaacctga cactgctctt tgtcaactta acgcataatg 28080
gagcatacat tggttataac aaagacggtt ctgatagaga attatatgag gtgtcagtca 28140
aaaccttgtt tcagaacggg gctggacaaa gtaaggttga acaaggtaat aaagggaaac 28200
ctaatactgc tcaaagtggt ggtaaaaaaa ccaaaacaga acatagaaac cagagtccaa 28260
aaagaaaatc aacaaataac cttcagccaa cacaattgta tgttaggcct tttactaatg 28320
ttagtttaac tggacctcca aatggcaagg ttacttggta tgatggcgaa cttaatgatc 28380
catgtgaaca aaagtacaaa ctcagaactt tttgcaatca gcaaaatcta actttaatta 28440
atgtaagcag cacttatgat ggcatctatt atggcactga tgaaaaagat aaggcaaatc 28500
gttacagaat aaaagtaaat actacaaatc acaaaactgt taaaattaag ccacatacca 28560
gagaacctcc tgctgtacaa gaaaaacagt ttgaattaca agatgcagaa actgatgaaa 28620
acgaatcaaa aattccctca gctactgtgg caatcgtggt gggagtgatt gcgggctttg 28680
taactctgat cattgtcttc atatgctaca tctgctgccg caagcgtccc aggtcataca 28740
atcatatggt agacccacta ctcagcttct cttactgaaa ctcagtcact ctcatttcag 28800
aaccatgaag gctttcacag cttgcgttct gattagcata gtcacactta gttcagctgc 28860
aatgattaat gttaatgtca ctagaggtgg taaaattaca ttgaatggga cttatccaca 28920
aactacatgg acaagatatc ataaagatgg atggaaaaat atctgtgaat ggaatgttac 28980
tgcatataaa tgcttcaata atggaagcat tactattact gccactgcca acattacttc 29040
tggcacatac aaagctgaaa gctataaaaa tgaaatcaaa aaactaacct ataaaaacaa 29100
caaaaccaca tttgaagatt ctggaaatta tgaacatcaa aaattatctt tttatatgtt 29160
gacaataatt gaactgccta caactaaggc tcccaccaca accagtacaa ctattaagac 29220
cactactcac actacagtgc aaaatactac tttattgatt gggtttttac tgagagagaa 29280
tgaaagtact actgaacaga cagaggctac ctcaagtgcc ttcagcagca ctgcaaattt 29340
aacttcgctt gcttggacta atgaaaccgg agtatcattg atgcatggcc agccttactc 29400
aggtttggat attcaaatta cttttctggt tgtctgtggg atctttattc ttgtggttct 29460
tctgtacttt gtctgctgca aagccagaga gaaatctagg aggcccatct acaggccagt 29520
gattggggaa cctcagccac tccaagtgga tggaggctta aggaatcttc tcttctcttt 29580
tacagtatgg tgatcagcca tgattcctag gttcttccta tttaacatcc tcttttgtct 29640
cttcaacatc tgtgctgcct tcgcggccgt ctcgcacgcc tcgcccgact gtctagggcc 29700
tttccccacc tacctcctct ttgccctgct cacctgcacc tgcgtctgca gcattgtctg 29760
cgtggtcatc accttcctgc agctcatcga ctggtgctgc gcgcgctata attatctcca 29820
ccacagtccc gaatacaggg acgagaacgt agccagaatc ttaaggctca tctgaccatg 29880
cagactctgc tcatgctgct atccctccta tcccctgccc tcgccacttc tgctgactct 29940
aaatgcaaat tcgcggagat atggaatttc ttagattgct atcaggagaa aattgatatg 30000
ccctcctatt acttggtgat tgtgggaata gtcatggtct gctcctgcac tttctttgcc 30060
atcatgatct acccctgttt tgatctcggc tggaactctg ttgaggcatt cacatacaca 30120
ctagaaagca gttcactagc ctccacgcca ccgcccacac cgcctccccg cagaaatcag 30180
ttcccactga ttcagtactt agaagagccc cctccccggc ccccttccac tgttagctac 30240
tttcacataa ccggcggcga tgactgacca ccacctggac ctcgagatgg acggccaggc 30300
ctccgagcag cgcatcctgc aactgcgcgt ccgtcagcag caggagcggg ccgccaagga 30360
gctcctcgat gccatcaaca tccaccagtg caagaagggc atcttctgcc ttgtcaaaca 30420
ggcaaagatc acctacgagc tcgtgtccgg cggcaagcag catcgcctca cctatgagct 30480
gccccagcag aagcagaagt tcacctgcat ggtgggcgtc aaccccatag tcatcaccca 30540
gcagtcgggc gagaccaacg gctgcatcca ctgctcctgc gaaagccccg agtgcatata 30600
ctccctcctc aagacccttt gcggactccg cgacctcctc cccatgaacg gaagcggagc 30660
tactaacttc agcctgctga agcaggctgg tgacgtcgag gagaatcctg gcccaatggt 30720
gagcaaaggc gaagagctgt tcaccggcgt ggtgcccatc ctggtggagc tggacggcga 30780
cgtgaacggc cacaagttca gcgtgagcgg cgagggcgag ggcgacgcca cctacggcaa 30840
gctgaccctg aagctgctgt gcaccaccgg caagctgccc gtgccctggc ccaccctggt 30900
gaccaccctg ggctacggcg tgcagtgctt cgcccggtac cccgaccaca tgaagcagca 30960
cgacttcttc aagagcgcca tgcccgaggg ctacgtgcag gagcggacca tcttcttcaa 31020
ggacgacggc aactacaaga cccgggccga ggtgaagttc gagggcgaca ccctggtgaa 31080
ccggatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg gccacaagct 31140
ggagtacaac tacaacagcc acaacgtgta catcaccgcc gacaagcaga agaacggcat 31200
caaggccaac ttcaagatcc ggcacaacat cgaggacggc ggcgtgcagc tggccgacca 31260
ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 31320
gagctaccag agcgccctgt tcaaggaccc caacgagaag cgggaccaca tggtgctgct 31380
ggagttcctg accgccgccg gcatcaccga gggcatgaac gagctctata agtgatgttg 31440
attaaaagca cgaaaaccaa tcagaccctt cccccatttc cccattccca attactcata 31500
aaataaatca ttggaattaa tcattcaata aaaatcactt acttgaaatc tgaaagtatg 31560
tctctggtgt agttgttcag cagcacctcg ttaccctcct cccagctctg gtattccagt 31620
ccccggcggg cggcgaactt cctccacacc ttgaaaggga tgtcaaattc ctggtccaca 31680
attttcattg tcttccctct cagatgtcaa agaggctccg ggtggaagat gacttcaacc 31740
ccgtctaccc ctatggctac gcgcggaatc agaatatccc cttcctcact cccccctttg 31800
tctcctccga tggattccaa aacttccccc ctggggtcct gtcactcaaa ctggctgacc 31860
caatagccat cgtcaatggg aatgtctcac tcaaagtggg agggggtctc actttgcaag 31920
atggaactgg aaaactaaca gtcaatgctg atccaccttt gcaacttaca aacaacaaat 31980
tagggattgc tttggacgct ccatttgatg ttatagacaa taaactcaca ttgttagcgg 32040
gccatggctt gtctattata acaaaagaaa catcaacact gcctggcttg attaatactc 32100
ttgtagtatt aactggaaag ggtattggaa cagaatcaac agataatggc ggaagcgtat 32160
gtgttagagt tggagaaggt ggcggcttat catttaataa tgatggagac ttggtagcat 32220
ttaataaaaa agaagataag cgcaccctat ggacaactcc agacacatct ccaaattgca 32280
agattgatca agataaggac tctaagttaa ctctggtcct tacaaagtgt ggaagtcaaa 32340
tattggctaa tgtgtcatta attgtcgtag ctggtaagta caaaattatc aataacaata 32400
ctcaaccagc tctcaaagga tttaccatta aattattgtt tgatgaaaat ggagtactta 32460
tggaatcttc aaatcttggt aaatcatatt ggaactttag aaatgaaaat tcaattatgt 32520
caacagctta tgaaaaagct attggattca tgcctaattt ggtagcctat ccaaaaccta 32580
ccgctggctc taaaaaatat gcaagagata tagtttatgg aaacatctac cttggtggaa 32640
agccagatca accagtaacc attaaaacta cctttaatca ggaaactgga tgtgaatatt 32700
ctatcacatt tgattttagt tgggccaaga cttatgtaaa tgttgaattt gaaacaacct 32760
cttttacctt ttcctatatc gcccaagaat gaaagaccaa taaacgtgtt tttcatttca 32820
aaattttaat catgtatctt tattgatttt tacaccagca cgggtagtca gtctcccacc 32880
accagcccat ttcacagtgt acactgttct ctcagcacgg gtggccttaa atagggaaat 32940
gttctcatta gtgcgggaac tggacttggg gtctataatc cacacagttt cctggcgagc 33000
caaacggggg tcggtgattg agatgaagcc gtcctctgaa aagtcatcca agcgagcctc 33060
acagtccaag gtcacagtct ggtggaatga gaagaacgca cagattcata ctcggaaaac 33120
aggatgggtc tgtgcctctc catcagcgcc ctcagcagtc tctgccgccg gggctcggtg 33180
cggctgctgc agattggatc gggatcgcaa gtctctctga ctataatccc cacagccttc 33240
agcatcagtc tcctggtgcg tcgggcacag caccgcatcc tgatctctgc catgttctca 33300
cagtaagtgc agcacataat caccatgtta ttcagcagcc cataattcag ggcgctccag 33360
ccaaagctca tgttggggat gatggaaccc acgtgaccat cgtaccagat gcggcagtat 33420
atcaggtgcc tgcccctcat aaacacactg cccatataca tgatctcttt gggcatgttt 33480
ctgttcacaa tctgacggta ccaggggaag cgctggttga acatgcaccc gtaaatgact 33540
ctcctgaacc acacggccag cagggtgcct cccgcccgac actgcaggga gcccggggat 33600
gaacagtggc aatgcaggat ccagcgctcg tacccgctca ccatctgagc tctcaccaag 33660
tccagggtag cagggcacag gcacactgac atacatcttt ttaaaatttt tatttcctct 33720
ggggtcagga tcatatccca ggggactgga aactcttgga gcagggtaaa gccagcagca 33780
catggtaatc cacggacaga acttacatta tgataatctg catgatcaca atcgggcaac 33840
aggggatgtt gttcagtcag tgaagccctg gtttcctcat cagatcgtgg taaacgggcc 33900
ctgcgatatg gatgatggcg gagcgagctg gattgaatct cggtttgcat tgtagtggat 33960
tctcttgcgt accttgtcgt acttctgcca gcagaaatgg gcccttgaac agcagatacc 34020
cctcctgcgg ccgtcctttc gctgctgccg ctcagtcatc caactgaagt acatccattc 34080
tcgaagattc tggagaagtt cctctgcatc tgatgaaata aaaaacccgt ccatgcgaat 34140
tcccctcatc acatcagcca ggactctgta ggccatcccc atccagttaa tgctgccttg 34200
tctatcattc agagggggcg gtggcaggat tggaagaacc atttttattc caaacggtct 34260
cgaaggacga taaagtgcaa gtcacgcagg tgacagcgtt cccctccgct gtgctggtgg 34320
aaacagacag ccaggtcaaa acccactcta ttttcaaggt gctcgaccgt ggcttcgagc 34380
agtggctcta cgcgtacatc cagcataaga atcacattaa aggctggccc tccatcgatt 34440
tcatcaatca tcaggttaca ttcctgcacc atccccaggt aattctcatt tttccagcct 34500
tgaattatct ctacaaattg ttggtgtaag tccactccgc acatgtggaa aagctcccac 34560
agtgccccct ccactttcat aatcaggcag accttcataa tagaaacaga tcctgctgct 34620
ccaccacctg cagcgtgttc aaaacaacaa gattcaataa ggttctgccc tccgccctga 34680
gctcgcgcct caatgtcagc tgcaaaaagt cacttaagtc ctgggccact acagctgaca 34740
attcagagcc agggctaagc gtgggactgg caagcgtaag ggaaaacttt aatgctccaa 34800
agctagcacc caaaaactgc atgctggaat aagctctctt tgtgtctccg gtgatgcctt 34860
ccaaaatgtg agtgataaag cgtggtagtt tttctttaat catttgcgta atagaaaagt 34920
cctctaaata agtcactagg accccaggga ccacaatgtg gtagcttaca ccgcgtcgct 34980
gaagcatggt tagtagagat gagagtctga aaaacagaaa gcatgcacta aactaaggtg 35040
gctattttta ctgaaggaaa aatcactctc tccagcaaca gggtacccac tgggtggccc 35100
ttgcggacat acaaaaatcg gtccgtgtga ttaaaaagca gcacagtaag ttcctgtctt 35160
cttccggcaa aaatcacatc ggactgggtt agtatgtccc tggcatggta gtcattcaag 35220
gccataaatc tgccctgata tccagtagga accagcacac tcacttttag gtgaagcaat 35280
accaccccat gcggaggaat gtggaaagat tcagggcaaa agaaggtata tctattgcta 35340
gtcccttcct ggacgggagc gatccctcca ggactatcta tgaaagcata cagagattca 35400
gccatagctc agcccgctta ccagtagaca gagagcacag cagtacaagc gccaacagca 35460
gcgactgact acccactaac ccagctccct atttaaaggc accttacact gacgtaatga 35520
ccaaaggtct aaaaaccccg ccaaaaaaac acacacgccc tgggtgtttt ttcgcgaaaa 35580
cacttccgcg ttctcacttc ctcgtatcga tttcgtgact caacttccgg gttcccacgt 35640
tacgtcactt ctgcccttac atgtaactca gtcgtagggc gccatgttgc ccacgtccaa 35700
aatggtttcc atgtccagcc acgcctccgc ggcgaccgtt agccgtgcgt cgtgacgtca 35760
tttgcatcgt cttctctcgt ccaatcagcg ttggctccgc cccgaaaccg ttaaaattca 35820
aaagctcatt tgcatattaa cttttgttta ctttgtgggg tatattatat agatag 35876
<210> 23
<211> 35563
<212> DNA
<213> 人工序列
<220>
<223> 合成腺病毒基因组Ad34 E3-14.8k-P2A-YPet
<400> 23
catcatcaat aatatacctt atagatggaa tggtgccaat atgtaaatga ggtgatttta 60
aaaattgtgg ggtgtgtggt gattggctgt ggggttaacg gctaaacggg gcggcgcggc 120
cgtgggaaaa tgacgttttg tgggggtgga gtttttttgc aagttgtcgc gggaaatgtg 180
acgcataaaa aggctttttt tctcacggaa ctactgactt ttcccacggt atttaacagg 240
aaatgaggta gttttgaccg gatgcaagtg aaaattgctg atttgcgcgc gaaaactgaa 300
tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360
ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420
ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctacggtatt 480
tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540
tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600
aatttctgct gagactggaa atgaaatact ggagcttgtg gtgcacgccc tgatgggaga 660
cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720
agaggtagag ggatcggagg attctaatga ggaagctgtg aatggctttt ttaccgattc 780
tatgctttta gctgctaatg aaggattaga attagatccg cctttggaca ctttcgatac 840
tccaggggtg attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgggttccgt 900
ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960
aaaggagcag tctatgcaga ctgcagcggg tgagggagtg aaggctgcca gtgttggttt 1020
tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080
aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagagcgc actgccactt 1140
tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200
attgagtggg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgagtcacc 1260
atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320
caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380
cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacggc caagacaata 1440
agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaga gtgcaatgta 1500
ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560
taagtagaag cagacctgta tggttagctc ataggagctg gctttcatcc atggaggttt 1620
gggccatttt ggaagacctt agaaagacta ggcaactgtt agaggacgct tcggacggag 1680
tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740
aacaggacta taaagaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800
ctcttaattt gggccatcaa gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860
caaccccagg tagaactgcc gctgctgtgg cttttcttac ttttatatta gataaatgga 1920
tcccgcagac tcatttcagc aggggatacg ttttggattt cgtagccaca gcattgtgga 1980
gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040
gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100
aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160
gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220
taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280
gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtc cagaaagagg gaagggatga 2340
agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400
tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460
acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520
ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580
gtggcctgga gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640
tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700
ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760
tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820
atgcatattc caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880
ctgcgcttct acagatactg gatgttttat tttaattaag ggcaatgcca gcgtaaagca 2940
taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgccggtgg 3000
gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060
ttttgatcac aatgtgttga ccaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120
tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180
cagaatgagc ctaacaggaa tctttgacat gaacatgcaa atctggaaga tcctgaggta 3240
tgatgatacg agatcgaggg tgcgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300
gccggtgtgt gtagatgtga ctgaagatct gagaccggat catttggtta ttgcccgcac 3360
tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420
ggggtggggt tttcagatgg acagattgag taaaaatttg ttttttctgt ctttcagctg 3480
tcatgagtgg aaacgcttct tttaaggggg gagtcttcag cccttatctg acagggcgtc 3540
tcccatcctg ggcaggagtt cgtcagaatg ttatgggatc tactgtggat ggaagacccg 3600
tccaacccgc caattcttca acgctgacct atgctacttt aagttcttca cctttggacg 3660
cagctgcagc cgccgccgcc gcctctgttg ccgctaacac tgtgcttgga atgggttact 3720
atggaagtat cgtggctaat tccacttcct ctaataaccc ttctaccctg actcaggaca 3780
agttacttgt ccttttggcc cagctggagg ctttgaccca acgtctgggt gaactttatc 3840
agcaggtggc cgagttgcga gtacaaactg agtctgctgt cggcacggca aagtctaaat 3900
aaaaaaaaat tccacaatca atgaataaat aaacgagctt gttgttgatt taaaatcaag 3960
tgtttttatt tcatttttcg cgcacggtat gccctagacc accgatctcg atcattgaga 4020
acacggtgga ttttttccag aatcctatag aggtgggatt gaatgtttag atacatgggc 4080
attaggccat ctttggggtg gagatagctc cattgaaggg attcatgctc cggggtagtg 4140
ttgtaaatca cccagtcata acaaggtcgc agtgcatggt gttgcacaat atcttttaga 4200
agtaggctga ttgccacaga taagcccttg gtgtaggtgt ttacaaaccg gttgagctgg 4260
gaggggtgca ttcggggtga aattatgtgc attttggatt ggatttttaa gttggcaata 4320
ttgccgccaa gatctcgtct tgggttcatg ttatgaagga ccaccaagac ggtgtatccg 4380
gtacatttag gaaatttatc gtgtagcttg gatggaaaag cgtggaaaaa tttggagaca 4440
cccttgtgtc ctccgagatt ttccatgcac tcatccatga taatagcaat ggggccgtgg 4500
gcagcagcgc gggcaaacac gttccgtggg tctgacacat catagttatg ttcctgagtt 4560
aaatcatcat aagccatttt aatgaatttg gggcggagag tacccgattg gggtatgaat 4620
gttccttcgg gccccggagc atagttcccc tcacagattt gcatttccca agctttcagt 4680
tccgatggtg gaatcatgtc cacctggggg gctatgaaga acaccgtttc tggggcgggg 4740
gtgattagtt gggatgatag caagtttctg agcaattgag atttgccaca tccggtgggg 4800
ccataaatga ttccgattac aggttgcagg tggtagttta gggaacggca actgccgtct 4860
tctcgaagca agggggccac ctcgttcatc atttccctta catgcatatt ttcccgcacc 4920
aaatccatta ggaggcgctc tcctcctagt gatagaagtt cttgtagtga ggaaaagttt 4980
ttcagcggtt ttagaccgtc agccatgggc attttggaga gagtttgctg caaaagttct 5040
agtctgttcc acagttcagt gatgtgttct atggcatctc gatccagcag acctcctcgt 5100
ttcgcgggtt tggacggctc ctggagtagg gtatgagacg atgggcgtcc agcgctgcca 5160
gggttcggtc cttccagggt ctcagtgttc gagtcagggt tgtttccgtc acagtgaagg 5220
ggtgtgcgcc tgcttgggcg cttgccaggg tgcgcttcag actcattctg ctggtggaga 5280
acttctgtcg cttggcgccc tgtatgtcgg ccaagtagca gtttaccatg agttcgtagt 5340
tgagcgcctc ggctgcgtgg cctttggcgc ggagcttacc tttggaagtt ttcttgcata 5400
ccgggcagta taggcatttc agcgcataca gcttgggcgc aaggaaaatg gattctgggg 5460
agtatgcatc tgcgccgcag gaggcgcaaa cagtttcaca ttccaccagc caggttaaat 5520
ccggttcatt ggggtcaaaa acaagttttc cgccatattt tttgatgcgt ttcttacctt 5580
tggtctccat gagttcgtgt cctcgttgag tgacaaacag gctgtccgta tccccgtaga 5640
ctgattttac aggcctcttc tccagtggag tgcctcggtc ttcttcgtac aggaactctg 5700
accactctga tacaaaggcg cgcgtccagg ccagcacaaa ggaggctatg tgggaggggt 5760
agcgatcgtt gtcaaccagg gggtccacct tttccaaagt atgcaaacac atgtcaccct 5820
cttcaacatc caggaatgtg attggcttgt aggtgtattt cacgtgacct ggggtccccg 5880
ctgggggggt ataaaagggg gcggttcttt gctcttcctc actgtcttcc ggatcgctgt 5940
ccaggaacgt cagctgttgg ggtaggtatt ccctctcgaa ggcgggcatg acctctgcac 6000
tcaggttgtc agtttctaag aacgaggagg atttgatatt gacagtgccg gttgagatgc 6060
ctttcatgag gttttcgtcc atttggtcag aaaacacaat ttttttattg tcaagtttgg 6120
tggcaaatga tccatacagg gcgttggata aaagtttggc aatggatcgc atggtttggt 6180
tcttttcctt gtccgcgcgc tctttggcag cgatgttgag ttggacatac tcgcgtgcta 6240
ggcacttcca ttcggggaag atagttgtca attcatctgg cacgattctc acttgccacc 6300
ctcgattatg caaggtaatt aaatccacac tggtggccac ctcgcctcga aggggttcgt 6360
tggtccaaca gagcctacct cctttcctag aacagaaagg gggaagtggg tctagcataa 6420
gttcatcggg agggtctgca tccatggtaa agattcccgg aagtaaatcc ttatcaaaat 6480
agctgatggg agtggggtca tctaaggcca tttgccattc tcgagctgcc agtgcacgct 6540
catatgggtt aaggggactg ccccagggca tgggatgggt gagtgcagag gcatacatgc 6600
cacagatgtc atagacgtag atgggatcct caaagatgcc tatataggtt ggatagcatc 6660
gcccccctct gatacttgct cgcacatagt catatagttc atgtgatggc gctagcaacc 6720
ccggacccaa gttggtgcga ttgggttttt ctgttctgta gacaatctgg cgaaagatgg 6780
cgtgagaatt ggaagagatg gtgggtcttt gaaaaatgtt gaaatgggca tgaggtagac 6840
ctacagagtc tctgacaaag tgggcataag attcttgaag cttggttacc agttcggcgg 6900
tgacaagtac gtctagggcg cagtagtcaa gtgtttcttg aatgatgtca taacctggtt 6960
ggtttttctt ttcccacagt tcgcggttga gaaggtattc ttcgcgatcc ttccagtact 7020
cttctagcgg aaacccgtct ttgtctgcac ggtaagatcc tagcatgtag aactgattaa 7080
ctgccttgta agggcagcag cccttctcta cgggtagaga gtatgcttga gcagcttttc 7140
gcagcgaagc gtgagtaagg gcgaaggtgt ctctgaccat gactttgaga aattggtatt 7200
tgaagtccat gtcgtcacag gctccctgtt cccagagttg gaagtctacc cgtttcttgt 7260
aggcggggtt gggcaaagcg aaagtaacat cgttgaagag aatcttaccg gctctgggca 7320
taaaattgcg agtgatgcgg aaaggctgtg gtacttccgc tcgattgttg atcacctggg 7380
cagctaggac gatctcgtcg aaaccgttga tgttgtgtcc tacgatgtat aattctatga 7440
aacgcggcgt gcctttgacg tgaggtagct tattgagctc atcaaaggtt aggtctgtag 7500
ggtcagataa ggcgtagtgt tcgagagccc attcgtgcag gtgaggattt gcatgtagga 7560
atgatgacca aagatccacc gccagtgctg tttgtaactg gtcccgatac tgacgaaaat 7620
gctggccaat tgccattttt tctggagtga cacagtagaa ggttctgggg tcttgttgcc 7680
atcgatccca ctttagttta atggctagat cgtgggccat gttgacgaga cgctcttctc 7740
ctgagagttt catgaccagc atgaaaggaa ctagttgttt gccaaaggac cccatccagg 7800
tgtaagtttc cacatcgtag gtcaggaaga gtctttctgt gcgaggatga gagccgatcg 7860
ggaagaactg gatttcctgc caccagttgg aggattggct gttgatgtga tggaagtaga 7920
agtttctgcg gcgcgccgag cattcgtgtt tgtgcttgta cagacggccg cagtagtcgc 7980
agcgttgcac gggttgtatc tcgtgaatga gctgtacctg gcttcccttg acgagaaatt 8040
tcagtgggaa gccgaggcct ggcgattgta tctcgtgctc ttctatattc gctgtatcgg 8100
cctgttcatc ttctgtttcg gtggtggtca tgctgacgag cccccgcggg aggcaagtcc 8160
agacctcggc gcgggagggg cggagctgaa ggaccagagc gcgcaggctg gagctgtcca 8220
gagtcctgag acgctgcgga ctcaggttag taggtaggga cagaagatta acttgcatga 8280
tcttttccag ggcgtgcggg aggttcagat ggtacttgat ttccacaggt tcgtttgtag 8340
agatgtcaat ggcttgcagg gttccgtgtc ctttgggcgc cactaccgta cctttgtttt 8400
ttcttttgat cggtggtggc tctcttgctt cttgcatgct cagaagcgat gacggggacg 8460
cgcgccgggc ggaagcggtt gttccggacc cggaggcatg gctggtagtg gcacgtcggc 8520
gccgcgcacg ggcaggttct ggtactgcgc tctgagaaga cttgcgtgcg ccaccacgcg 8580
tcgattgacg tcttgtatct gacgtctctg ggtgaaagct accggccccg tgagcttgaa 8640
cctgaaagag agttcaacag aatcaatttc ggtatcgtta acggcagctt gtctcagtat 8700
ttcttgtacg tcaccagagt tgtcctggta ggcgatctcc gccatgaact gctcgatttc 8760
ttcctcctga agatctccgc gacccgctct ctcgacggtg gccgcgaggt cattggagat 8820
acggcccatg agttgggaga atgcagtcat gcccgcctcg ttccagacgc ggctgtaaac 8880
cacggccccc tcggagtctc ttgcgcgcat caccacctga gcgaggttaa gctccacgtg 8940
tctggtgaag accgcatagt tgcataggcg ctgaaaaagg tagttgagtg tggtggcaat 9000
gtgttcggcg acgaagaaat acatgatcca tcgtctcagc ggcatttcgc tgacatcgcc 9060
cagagcttcc aagcgctcca tggcctcgta gaagtccacg gcaaaattaa aaaactggga 9120
gtttcgcgcg gacacggtca attcctcctc gagaagacgg atgagttcgg ctatggtggc 9180
ccgtacttcg cgttcgaagg ctcccgggat ctcttcttcc tcttctatct cttcttccac 9240
taacatctct tcttcgtctt caggcggggg cggagggggc acacggcgac gtcgacggcg 9300
cacgggcaaa cggtcgatga atcgttcaat gacctctccg cggcggcggc gcatggtttc 9360
agtgacggcg cggccgttct cgcgcggtcg cagagtaaaa acaccgccgc gcatctcctt 9420
aaagtggtga ctgggaggtt ctccgtttgg gagggagagg gcgctgatta tacattttat 9480
taattggccc gtagggactg cgcgcagaga tctgatcgtg tcaagatcca cgggatctga 9540
aaacctttcg acgaaagcgt ctaaccagtc acagtcacaa ggtaggctga gtacggcttc 9600
ttgtgggcgg gggtggttat gtgttcggtc tgggtcttct gtttcttctt catctcggga 9660
aggtgagacg atgctgctgg tgatgaaatt aaagtaggca gttctaagac ggcggatggt 9720
ggcgaggagc accaggtctt tgggtccggc ttgctggata cgcaggcgat tggccattcc 9780
ccaagcatta tcctgacatc tagcaagatc tttgtagtag tcttgcatga gccgttctac 9840
gggcacttct tcctcacccg ttctgccatg catacgtgtg agtccaaacc cgcgcattgg 9900
ttgtaccagt gccaagtcag ctacgactct ttcggcgagg atggcttgct gtacttgggt 9960
gagggtggct tgaaagtcat caaaatccac aaagcggtgg taagccccgg tattaatggt 10020
gtaagcacag ttggccatga ctgaccagtt aactgtctgg tgaccagggc gcacgagctc 10080
ggtgtattta aggcgcgaat aggcgcgggt gtcaaagatg taatcgttgc aggtgcgcac 10140
cagatactgg taacctataa gaaaatgcgg cggtggttgg cggtagagag gccatcgttc 10200
tgtagctgga gcgccggggg cgaggtcttc caacataagg cggtgatagc cgtagatgta 10260
cctggacatc caggtgattc ctgcggcggt agtagaagcc cgaggaaact cgcgtacgcg 10320
gttccaaatg ttgcgtagcg gcatgaagta gttcattgta ggcacggttt gaccagtgag 10380
gcgcgcgcag tcattgatgc tctatagaca cggagaaaat gaaagcgttc agcgactcga 10440
ctccgtagcc tggaggaacg tgaacgggtt gggtcgcggt gtaccccggt tcgagacttg 10500
tactcgagcc ggccggagcc gcggctaacg tggtattggc actcccgtct cgacccagcc 10560
tacaaaaatc caggatacgg aatcgagtcg ttttgctggt tgccgaatgg cagggaagtg 10620
agtcctattt tttttttttg ccgctcagat gcatcccgtg ctgcgacaga tgcgtcccca 10680
acaacagccc ccctcgcagc agcagcaacc acaaaaggct gtccctgcaa ctactgcaac 10740
tgccgctgtg agcggtgcgg gacagcccgc ctatgatctg gacttggaag agggcgaagg 10800
actggcacgt ctaggtgcgc cttcgcccga gcggcatccg cgagttcaac tgaaaaaaga 10860
ttctcgcgag gcgtatgtgc cccaacagaa cctatttaga gacagaagcg gcgaggagcc 10920
ggaggagatg cgagcttccc gctttaacgc gggtcgtgag ctgcgtcacg gtttggacag 10980
aagacgagtg ttgcgggacg aggatttcga agttgatgaa gtgacaggga tcagtcctgc 11040
cagggcacac gtggctgcag ccaaccttgt atcggcttac gaacagacag taaaggaaga 11100
gcgtaatttc caaaagtctt ttaataatca tgtgcgaacc ctcattgccc gcgaagaagt 11160
cacccttggt ttgatgcatt tgtgggattt gatggaagct atcattcaga accctactag 11220
caaacctctg accgcacagc tgtttctggt ggtgcaacac agcagagaca atgaggcttt 11280
cagagaggcg ctgctcaaca tcaccgaacc cgaggggaga tggttgtatg atcttatcaa 11340
cattctacag agtatcatag tgcaggagcg gagcctgggc ctggccgaga aggtggctgc 11400
catcaattac tcggttttga gcttgggaaa gtattacgct cgcaagatct acaagactcc 11460
atacgttccc atagacaagg aggtgaagat agatgggttc tacatgcgca tgacgctgaa 11520
ggtgttgacc ctgagcgatg atcttggggt gtaccgcaat gacagaatgc atcgcgcggt 11580
gagcgccagc aggaggcgcg agttaagcga cagggaactg atgcacagtt tgcaaagagc 11640
tctaactgga gctggaaccg agggtgagaa ttactttgat atgggagctg acttgcagtg 11700
gcagcctagt cgcagggctc tgaacgccgc gacggcagga tgtgagcttc cttacataga 11760
agaggcggat gaaggcgagg aggaagaggg cgagtacttg gaagactgat ggcacaaccc 11820
gtgttttttg ctagatggaa cagcaagcac cggatcccgc aatgcgggcg gcgctgcaga 11880
gccagccgtc cggcattaac tcctcggacg attggaccca ggccatgcaa cgtatcatgg 11940
cgttgacgac tcgcaacccc gaagccttta gacagcaacc ccaggccaac cgtctatcgg 12000
ccatcatgga agctgtagtg ccttcccgct ctaatcccac tcatgagaag gtcctggcca 12060
tcgtgaacgc gttggtggag aacaaagcta ttcgtccaga tgaggccgga ctggtataca 12120
acgctctctt agaacgcgtg gctcgctaca acagtagcaa tgtgcaaacc aatttggacc 12180
gtatgataac agatgtacgc gaagccgtgt ctcagcgcga aaggttccag cgcgatgcca 12240
acctgggttc gctggtggcg ttaaatgctt tcttgagtac tcagcctgct aatgtgccgc 12300
gtggtcaaca ggattatact aactttttaa gtgctttgag actgatggta tcagaagtac 12360
ctcagagcga agtatatcag tccggtcctg attacttctt tcagactagc agacagggct 12420
tgcagacggt aaatctgagc caagctttta aaaaccttaa aggtttgtgg ggagtgcatg 12480
ccccggtagg agaaagagca accgtgtcta gcttgttaac tccgaactcc cgcctattat 12540
tactgttggt agctcctttc accgacagcg gtagcatcga ccgtaattcc tatttgggtt 12600
acctactaaa cctgtatcgc gaagccatag ggcaaagtca ggtggacgag cagacctatc 12660
aagaaattac ccaagtcagt cgcgctttgg gacaggaaga cactggcagt ttggaagcca 12720
ctctgaactt cttgcttacc aatcggtctc aaaagatccc tcctcaatat gctcttactg 12780
cggaggagga gaggatcctt agatatgtgc agcagagcgt gggattgttt ctgatgcaag 12840
agggggcaac tccgactgca gcactggaca tgacagcgcg aaatatggag cccagcatgt 12900
atgccagtaa ccgacctttc attaacaaac tgctggacta cttgcacaga gctgccgcta 12960
tgaactctga ttatttcacc aatgccatct taaacccgca ctggctgccc ccacctggtt 13020
tctacacggg cgaatatgac atgcccgacc ctaatgacgg atttctgtgg gacgacgtgg 13080
acagcgatgt tttttcacct ctttctgatc atcgcacgtg gaaaaaggaa ggcggcgata 13140
gaatgcattc ttctgcatcg ctgtccgggg tcattggtgc taccgcggct gagcccgagt 13200
ctgcaagtcc ttttcctagt ctaccctttt ctctacacag tgtacgtagc agcgaagtgg 13260
gtagaataag tcgcccgagt ttaatgggcg aagaggagta cctaaacgat tccttgctca 13320
gaccggcaag agaaaaaaat ttcccaaaca atggaataga aagtttggtg gataaaatga 13380
gtagatggaa gacttatgct caggatcaca gagacgagcc tgggatcatg gggactacaa 13440
gtagagcgag ccgtagacgc cagcgccatg acagacagag gggtcttgtg tgggacgatg 13500
aggattcggc cgatgatagc agcgtattgg acttgggtgg gagaggaagg ggcaacccgt 13560
ttgctcattt gcgccctcgc ttgggtggta tgttgtaaaa aaaaataaaa aagaaaaaac 13620
tcaccaaggc catggcgacg agcgtacgtt cgttcttctt tattatctgt gtctagtata 13680
atgaggcgag tcgtgctagg cggagcggtg gtgtatccgg agggtcctcc tccttcgtac 13740
gagagcgtga tgcagcagca gcaggcgacg gcggtgatgc aatccccact ggaggctccc 13800
tttgtgcctc cgcgatacct ggcacctacg gagggcagaa acagcattcg ttactcggaa 13860
ctggcacctc agtacgatac caccaggttg tatctggtgg acaacaagtc ggcggacatt 13920
gcttctctga actatcagaa tgaccacagc aacttcttga ccacggtggt gcaaaacaat 13980
gactttaccc ctacggaagc cagcacccag accattaact ttgatgaacg atcgcggtgg 14040
ggcggtcagc taaaaaccat catgcatact aacatgccca acgtgaacga gtatatgttt 14100
agtaacaagt tcaaagcgcg tgtgatggtg tccagaaaac ctcctgaggg tgttagagta 14160
gacgataatt atgatcataa gcaagatatt ctaaaatacg agtggttcga gtttactttg 14220
ccagaaggca acttttcggt cactatgact atcgacttga tgaacaatgc catcatagac 14280
aattacttga aagtgggcag acagaatgga gtgttggaaa gtgacattgg tgttaagttc 14340
gacactagga acttcaagtt gggatgggat ccagaaacta agttgatcat gcctggggtt 14400
tacacctatg aggccttcca tcctgacatc gtattgctgc ctggctgcgg agtggacttt 14460
accgaaagcc gtctgagcaa ccttcttggc attagaaaga aacacccatt ccaagagggt 14520
tttaagatct tgtatgagga tttagaagga ggaaatattc cagccctttt ggatgtagat 14580
gcttatgaga acagcaagaa agatcaaaaa gccaaaatag aagctgctgc agaagctaaa 14640
gcaaacatag ttgccaacga tccggtaagg gtggctaacg ctagtgaaat caggggagac 14700
agttttgccg caacatccgt tccgactaaa gaatcattat tggatgatgt gtctcaaaac 14760
atagagttaa aactcactat taagcctgtg gaaaaagatg gcaaaaacag aagttacaat 14820
gtgttggaag ataaaatcaa cacggcctat cgcagttggt acctttcgta caattatggc 14880
gaccccgaaa aaggagtgcg ttcctggaca ttgctcacca cctcagatgt cacctgcgga 14940
gcggagcagg tctactggtc gcttccagac atgatgcagg atcctgtcac tttccgctcc 15000
actagacaag tcagtaacta ccctgtggtg ggtgcagagc ttatgcccgt cttttcaaag 15060
agcttctaca acgaacaagc tgtgtactcc cagcagctcc gccagtccac ctcgcttacg 15120
cacgtcttca accgctttcc tgagaaccag attttaatcc gtccgccggc gcccacaatt 15180
accaccgtca gtgaaaacgt tcctgctctc acagatcacg ggaccctgcc gttgcgcagc 15240
agtatccggg gagtccaacg tgtgaccgtt actgacgcca gacgccgcac ctgtccctac 15300
gtgtacaagg cactgggcat agtcgcaccg cgcgtccttt caagccgcac tttctaaaaa 15360
aaaaaaaaaa atgtccgttc ttatctcgcc cagtaataac accggttggg gtctgcgcgc 15420
tcccagcaag atgtacggag gcgcacgcaa acgttctacc caacatcccg tgcgtgttcg 15480
cgggcatttt cgcgctccat ggggtgccct caagggccgc actcgcgttc gaaccaccgt 15540
cgatgatgta atcgatcagg tggttgccga cgcccgtaat tatactccta ctgcgcctac 15600
atctactgtg gacgcagtta ttgacagtgt agtggctgac gctcgcaact atgctcgacg 15660
taagagccgg cgaaggcgca ttgccagacg tcaccgagct accactgcca tgcgagcagc 15720
aagagctctg ctacgaagag ctagacgcgt ggggcgaaga gccatgctta gggcggccag 15780
acgtgcagct tcgggcgcca gcgccggcag gtcccgcagg caagcagccg ctgtcgcagc 15840
ggcgactatt gccgacatgg cccaatcgcg aagaggcaat gtatactggg tgcgtgacgc 15900
tgccaccggt caacgtgtac ccgtgcgcac ccgtccccct cgcacttaga agatactgag 15960
cagtctccga tgttgtgtcc cagcggcgag gatgtccaag cgcaaataca aggaagaaat 16020
gctgcaggtt atcgcacctg aagtctacgg ccaaccgttg aaggatgaaa aaaaaccccg 16080
caaaatcaag cgggtaaaaa aggacaaaaa agaagaggaa gatggcgatg atgggctggc 16140
ggagtttgtg cgcgagtttg ccccacggcg acgcgtgcaa tggcgtgggc gcaaagttcg 16200
acatgtgttg agacctggaa cttcggtggt ctttacaccc ggcgagcgtt caagcgctac 16260
ttttaagcgt tcctatgatg aggtgtacgg ggatgatgat attcttgagc aggcagctga 16320
ccgattaggc gagtttgctt atggcaagcg tagtagaata aatcccaagg atgaaacagt 16380
gtccataccc ttggatcatg gaaatcccac ccctagtctt aaaccggtca ctttgcagca 16440
agtgttaccc gtaactccgc gaacaggtgt taaacgcgaa ggtgaagatt tgtatcccac 16500
tatgcaactg atggtgccca aacgccagaa gttggaggac gttttggaga aagtaaaagt 16560
ggatccagat attcaacctg aggttaaagt gagacccatt aagcaggtag cgcctggtct 16620
gggagtacaa actgtagaca ttaaaattcc cactgaaagt atggaagtgc aaactgaacc 16680
cgcaaagcct actgccacct ccactgaagt gcaaacggac ccatggatgc ccatgcctat 16740
tacaactgac gccgtcggtc ccactcgaag atcccgacga aagtacggtc cagcaagtct 16800
gttgatgccc aactatgtcg tacacccatc tattattcct actcctggtt accgaggcac 16860
tcgctactat cgcagccgaa acagtacttc ccgccgtcgc cgcaagacac ctgcaaatcg 16920
cagtcgtcgc cgtagacgca caagcaaacc gattcccggc gccctggtgc ggcaagtgta 16980
ccgcaatggt agtgcggaac ctttgacact gccgcgtgcg cgttaccatc ctagtatcat 17040
cacttaatca atgttgccgc tgcctccttg cagatatggc cctcacttgt cgccttcgcg 17100
ttcccatcac tggttaccga ggaagaaact cgcgccgtag aagagggatg ttggggcgcg 17160
gaatgcgacg ctacaggcga cggcgtgcta tccgcaagca attgcggggt ggttttttgc 17220
cagccttaat tccaattatc gctgctgcga ttggcgcaat accaggcata gcttccgtgg 17280
cggttcaggc ctcgcaacga cattgacatt ggaaaaaaaa aaaacgtata aataaaaaat 17340
acaatggact ctgacactcc tggtactgtg actatgtttt cttagagatg gaagacatca 17400
atttttcatc cttggctccg cgacacggca cgaagccgta catgggcacc tggagcgaca 17460
tcggcacgag ccaactgaac gggggcgcct tcaattggag cagtatctgg agcgggctta 17520
aaaattttgg ctcaaccata aaaacatacg ggaacaaagc ttggaacagc agtacaggac 17580
aggcgcttag aaataaactt aaagaccaga acttccaaca aaaagtagtc gatgggatag 17640
cttccggtat caatggagtg gtagatttgg ctaaccaggc tgtgcagaaa aagataaaca 17700
gtcgtttgga cccgccgcca gcaaccccag gtgaaatgca agtggaggaa gaaattcctc 17760
cgccagaaaa acgaggcgac aagcgtccgc gtcccgattt ggaagagacg ctggtgacgc 17820
gcgtagatga accgccttct tatgaggaag caacgaagct tggaatgccc accactagac 17880
cgatagcccc tatggccacc ggggtgatga aaccttctca gttgcatcga cccgtcacct 17940
tggatttgcc ccctcctcct gctgctactg ctgtacccgc ttctaagcct gtcgctgccc 18000
cgaaaccagt cgccgtagcc aggtcacgtc ccgggggcgc tcctcgtcca aatgcacact 18060
ggcaaaatac tctgaacagc atcgtgggtc taggcgtgca aagtgtaaaa cgccgtcgct 18120
gcttttaatt aaatatggag tagcgcttaa cttgcctatc tgtgtatatg tgtcattaca 18180
cgccgtcaca gcatcagagg aaaaaaggaa gaggtcgtgc gtcgacgctg agttactttc 18240
aagatggcca ccccatcgat gctgccccaa tgggcataca tgcacatcgc cggacaggat 18300
gcttcggagt acctgagtcc gggtctggtg cagttcgccc gcgccacaga cacctacttc 18360
aatctgggaa ataagtttag aaatcctacc gtagcgccga cccacgatgt gaccaccgat 18420
cgtagccagc ggctcatgtt gcgcttcgtg cccgttgacc gggaggacaa tacatactct 18480
tacaaagtgc ggtacaccct ggccgtgggc gacaacagag tgctggatat ggccagcacg 18540
ttctttgaca ttaggggcgt gttggacaga ggtcccagtt ttaaacccta ttctggtacg 18600
gcttacaact ccctggctcc taaaggcgct ccaaatgcat ctcagtggtt ggataaggga 18660
gttacaagca ctggcctagt ggacgacggc aatactgatg atggggaaga agccaaaaaa 18720
gcaacataca cttttggtaa tgctccagta aaagccgagg ctgaaatcac aaaagacgga 18780
ttgccggtgg gcttggaagt ttcaactgaa ggtcctaaac caatctatgc tgataagctt 18840
tatcagccag aacctcaagt gggagacgaa acttggactg acctagacgg aaaaaccgaa 18900
gagtatggag ggagggttct taaacctgaa actaaaatga aaccctgcta cggatctttt 18960
gctaaaccta ctaatattaa aggaggtcag gcaaaggtaa aaccaaaaga agacgatggc 19020
actaacaaca tcgagtatga cattgacatg aacttctttg acttaagatc acaaagatca 19080
gaactcaaac ctaaaattgt aatgtatgca gaaaatgtgg acctggaatg tccagatact 19140
catgttgtgt acaaacctgg agtttcagat gctagttctg agaccaatct tggacaacag 19200
tctatgccca acagacccaa ctacattggc ttcagagata acttcatcgg acttatgtac 19260
tataacagta ctggcaacat gggggtactg gctggccaag cgtctcagtt gaatgcagtg 19320
gttgacttgc aggacagaaa cacagaactg tcttaccaac tcttgcttga ctctctgggc 19380
gacagaacca gatactttag catgtggaat caggctgtgg acagttatga tcctgatgta 19440
cgtgttattg aaaatcatgg tgtggaagat gaacttccca actattgttt tccgttggat 19500
ggtgtcggtc cgcgaacaga tagttacaag gagattaagc caaatggaga ccaatctact 19560
tggacaaatg tagacccaac tggcagcagt gaacttgcta agggaaatcc atttgccatg 19620
gaaattaacc ttcaagccaa tctatggcga agtttccttt attccaatgt ggctctatat 19680
ctcccagact cgtacaaata caccccgtcc aatgtcactc ttccagaaaa caaaaacacc 19740
tacgactaca tgaacgggcg ggtggtgccg ccatctctag tagacaccta tgtgaacatt 19800
ggtgccaggt ggtctctgga tgccatggac aatgtcaacc cattcaacca ccaccgtaac 19860
gctggcttgc gttaccgatc catgcttctg ggtaacggac gttatgtgcc tttccacata 19920
caagtgcctc aaaaattctt cgctgttaaa aacctgctgc ttctcccagg ctcctacact 19980
tatgagtgga actttaggaa ggatgtaaac atggttctac agagttccct cggtaacgac 20040
ctacgggtag atggcgccag catcagtttt acgagcatca acctctatgc tacttttttc 20100
cccatggctc acaacaccgc ttccaccctt gaagccatgc tgcggaatga caccaatgat 20160
cagtcattca acgactacct atctgcagct aacatgctct accccattcc tgccaatgca 20220
accaatattc ccatttccat tccttctcgc aactgggcgg ctttcagagg ctggtcattt 20280
accagactga aaaccaaaga aactccctct ttggggtctg gatttgaccc ctacttcgtc 20340
tattctggtt ctattcccta cctggatggt accttctacc tgaaccacac ttttaagaag 20400
gtttccatca tgtttgactc ttcagtgagc tggcctggaa atgacaggtt actatctcct 20460
aacgaatttg aaataaagcg cactgtggat ggcgaaggct acaacgtagc ccaatgcaac 20520
atgaccaaag actggttctt ggtacagatg ctcgccaact acaacatcgg ctatcagggc 20580
ttctacattc cagaaggata caaagatcgc atgtattcat ttttcagaaa cttccagccc 20640
atgagcaggc aggtggttga tgaggtcaat tacaaagact tcaaggccgt cgccataccc 20700
taccaacaca acaactctgg ctttgtgggt tacatggctc cgaccatgcg tcaaggtcaa 20760
ccctatcccg ctaactatcc ctatccactc attggaacaa ctgccgtaaa tagtgttacg 20820
cagaaaaagt tcttgtgtga cagaaccatg tggcgcatac cgttctcaag caacttcatg 20880
tctatgggag cccttacaga cttgggacag aacatgctct atgccaactc agctcatgct 20940
ctggacatga cctttgaggt ggatcccatg gatgagccca ccctgcttta tcttctcttc 21000
gaagttttcg acgtggtcag agtgcatcag ccacaccgcg gcatcatcga ggcagtctac 21060
ctgcgtacac cgttctcggc cggtaacgct accacgtaag aagcttcttg cttcttgcaa 21120
acagcagctg caaccatggc ctgcggatcc caaaacggct ccagcgagca agagctcaga 21180
gccattgtcc aagacctggg ttgcggacca tattttttgg gaacctttga taagcgcttc 21240
ccggggttca tggcccccga taagctcgcc tgtgccattg taaatacggc cggacgtgag 21300
acggggggag agcactggtt ggctttcggt tggaacccac gttctaacac ctgctacctt 21360
tttgatcctt ttggattctc ggatgatcgt ctcaaacaga tttaccagtt tgaatatgag 21420
ggtctcctgc gccgcagcgc tcttgctacc aaggaccggt gtattacgct ggaaaaatct 21480
acccagaccg tgcagggccc ccgttctgcc gcctgcggac ttttctgctg catgttcctt 21540
catgcctttg tgcactggcc tgaccgtccc atggacggaa accccaccat gaaattgcta 21600
actggagtgc caaacaacat gcttcattct cctaaagtcc agcccaccct gtgtgacaat 21660
caaaaagcac tctaccattt tctcaatacc cattcgcctt attttcgctc tcatcgtaca 21720
cacatcgaaa gggccactgc gttcgaccgt atggatgtgc aataatgatt catgtaaaca 21780
acgtgttcaa taaacagcac tttatttttt acatgtatcg aggctctgga ttacttattt 21840
atttacaagt cgaatgggtt ctgacgagaa tcagaatgac ccgcaggcag tgatacgttg 21900
cggaactgat acttgggttg ccacttgaat tcgggaatca ccaacttggg aaccggtata 21960
tcgggcagga tgtcactcca cagctttctg gtcagctgca aagctcccag caggtcagga 22020
gccgaaatct tgaaatcaca attaggacca gtgctctgag cgcgagagtt gcggtacacc 22080
ggattgcagc actgaaacac catcagcgac ggatgtctta cgcttgccag cacggtggga 22140
tctgcaatca tgcccacatc cagatcttca gcattggcaa tgctgaacgg ggtcatcttg 22200
caggtctgcc tacccatggc gggcacccaa ttaggcttgt ggttacaatc gcagtgcagg 22260
gggatcagta tcatcttggc ctgatcctgt ctgattcctg gatacacggc tctcatgaaa 22320
gcatcatatt gcttgaaagc ctgctgggct ttactaccct cggtataaaa catcccgcag 22380
gacctgctcg aaaactggtt agctgcgcag ccggcatcat tcacacagca gcgggcgtca 22440
ttgttggcta tttgcaccac acttctgccc cagcggtttt gggtgatttt ggttcgctcg 22500
ggattctcct tcaaggctcg ttgtccgttc tcgctggcca catccatctc gataatctgc 22560
tccttctgaa tcataatatt gccatgcaag cacttcagct tgccctcata atcattgcag 22620
ccatgaggcc acaacgcaca gcctgtacat tcccaattat ggtgggcgat ctgagaaaaa 22680
gaatgtatca ttccctgcag aaatcttccc atcatcgtgc tcagtgtctt gtgactagtg 22740
aaagttaact ggatgcctcg gtgctcctcg ttcacgtact ggtgacagat gcgcttgtat 22800
tgttcgtgct gctcaggcat tagtttaaaa gaggttctaa gttcgttatc cagcctgtac 22860
ttctccatca gcagacacat cacttccatg cctttctccc aagcagacac caggggcaag 22920
ctaatcggat tcttaacagt gcaggcagca gctcctttag ccagagggtc atctttggcg 22980
atcttctcaa tgcttctttt gccatccttc tcaacgatgc gcacgggcgg gtagctgaaa 23040
cccactgcta caagttgcgc ctcttctctt tcttcttcgc tgtcttgact gatgtcttgc 23100
atggggacat gtttggtctt ccttggcttc tttttcgggg gtatcggagg aggaggactg 23160
tcgctccgtt ccggagacag ggaggattgt gacgtttcgc tcaccattac caactgactg 23220
tcggtagaag aacctgaccc cacacggcga caggtgtttc tcttcggggg cagaggtgga 23280
ggcgattgcg aagggctgcg gtccgacctg gaaggcggat gactggcaga accccttccg 23340
cgttcggggg tgtgctccct gtggcggtcg cttaactgat ttccttcgcg gctggccatt 23400
gtgttctcct aggcagagaa acaacagaca tggaaactca gccattgctg tcaacatcgc 23460
cacgagtgcc atcacatctc gtcctcagcg acgaggaaaa ggagcagagc ttaagcattc 23520
caccgcccag tcctgccacc acctctaccc tagaagataa ggaggtcgac gcatctcatg 23580
acatgcagaa taaaaaagcg aaagagtctg agccagacat cgaacaagac ccgggctatg 23640
tgacaccggt ggaacacgag gaagagttga aacgctttct agagagagag gatgaaaact 23700
gcccaaaaca gcaagcggat aactatcacc aagatgctgg aaatagggat cagaacaccg 23760
actacctcat agggcttgac ggggaagacg cgctccttaa acatctagca agacagtcac 23820
tcatagtcaa ggatgcatta ttggacagaa ctgaagtgcc catcagtgtc gaagagctca 23880
gccgcgccta cgagcttaac ctattttcac ctcgtactcc ccccaaacgt cagccaaacg 23940
gcacctgcga gccaaatcct cgcttaaact tttatccagc ttttgctgtg ccagaagtac 24000
tggctaccta tcacatcttt tttaaaaatc aaaaaattcc agtctcctgc cgcgctaatc 24060
gcacccgcgc cgatgcccta ctcaatctgg gacctggttc acgcttacct gatatagctt 24120
ccttggaaga ggttccaaag atcttcgagg gtctgggcaa taatgagact cgggccgcaa 24180
atgctctgca aaagggagaa aatggcatgg atgagcatca cagcgttctg gtggaattgg 24240
aaggcgataa tgccagactc gcagtactca agcgaagcgt cgaggtcaca cactttgcat 24300
accccgctgt caacctgccc cctaaagtca tgacggccgt catggaccag ttactcatta 24360
agcgcgcaag tcccctttca gaagacatgc atgacccaga tgcctgtgat gagggtaaac 24420
cagtggtcag tgatgagcag ctaacccgat ggctgggcac cgactctccc cgggatttgg 24480
aagagcgtcg caagcttatg atggccgtgg tgctggttac cgtagaacta gagtgtcttc 24540
ggcgtttctt taccgattca gaaaccttgc gcaaactcga agagaatctg cactacactt 24600
ttagacacgg ctttgtgcgg caggcatgca agatatctaa cgtggaactc accaacctgg 24660
tttcctacat gggtattctg catgagaatc gcctaggaca aagcgtgctg cacagcaccc 24720
ttaaggggga agcccgccgt gattacatcc gcgattgtgt ttatctctac ctgtgccaca 24780
cgtggcaaac cggcatgggt gtatggcagc aatgtttaga agaacagaac ctgaaagagc 24840
taaacaagct cttacagaaa tctcttaagg ttctgtggac agggttcgac gagcgcaccg 24900
tcgcttccga cctggcagac ctcatcttcc cagagcgtct cagggttact ttgcgaaacg 24960
gactgcctga ctttatgagc cagagcatgc ttaacaattt tcgctctttc atcctggaac 25020
gctccggtat cctgcccgcc acctgctgcg cactgccctc cgactttgtg cctctcacct 25080
accgcgaatg ccccccgccg ctatggagtc actgctacct gttccgtctg gccaactacc 25140
tctcctacca ctcggatgtg atcgaggatg tgagcggaga cggcttgctg gagtgtcact 25200
gccgctgcaa tctgtgcacg ccccaccggt ccctagcttg caacccccag ttgatgagcg 25260
aaacccagat aataggcacc tttgaattgc aaggccccag cagccaaggc gatgggtctt 25320
ctcctgggca aagtttaaaa ctgaccccgg gactgtggac ctccgcctac ttgcgcaagt 25380
ttgccccgga agattaccac ccctatgaaa tcaagttcta tgaggaccaa tcacagcctc 25440
cgaaagccga actttcggcc tgcgtcatca cccagggggc aattctggcc caattgcaag 25500
ccatccaaaa atcccgccaa gaatttctac tgaaaaaggg taagggggtc taccttgacc 25560
cccagaccgg cgaggaactc aacacaaggt tccctcagga tgtcccaacg acgagaaagc 25620
aagaagttga aggtgcagcc gccgccccca gaagatatgg aggaagattg ggacagtcag 25680
gcagaggaag cggaggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740
gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800
gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860
cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920
ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980
tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040
ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100
agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160
accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220
agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280
catcttccag cagagtcggg gccaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340
ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400
ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460
cgcgcttatt caaaaaaggc gggaattaca tcatcctcga cgtttatgag taaagaaatt 26520
cccacgcctt acatgtggag ttatcagccc caaatgggat tggcggcagg cgcctcccag 26580
gactactcca cccgcatgaa ttggctcagc gccgggcctt ctatgatttc tcgagttaat 26640
gatatacgcg cctaccgaaa ccaaatactt ttggaacagt cagctcttac caccacgccc 26700
cgccaacacc ttaatcccag aaattggccc gccgccctag tgtaccagga aagtcccgct 26760
cccaccactg tattacttcc tcgagacgcc caggccgaag tccaaatgac taatgcaggt 26820
gcgcagttag cgggcggctc caccctatgt cgtcacaggc ctcggcataa tataaaacgc 26880
ctgatgatca gaggccgagg tatccagctc aacgacgagt cggtgagctc tccgcttggt 26940
ctacgaccag acggaatctt tcagattgcc ggctgcggga gatcttcctt cacccctcgt 27000
caggctgttc tgactttgga aagttcgtct tcgcaacccc gctcgggcgg aatcgggacc 27060
gttcaatttg tggaggagtt tactccctct gtctacttca accccttctc cggatctcct 27120
gggcactacc cggacgagtt cataccgaac ttcgacgcga ttagcgagtc agtggacggc 27180
tacgattgat gtctggtgac gcggctgagc tatctcggct gcgacatcta gaccactgcc 27240
gccgctttcg ctgctttgcc cgggaactca ttgagttcat ctacttcgaa ctccccaagg 27300
atcaccctca aggtccggcc cacggagtgc ggattactat cgaaggcaaa atacactctc 27360
gcctgcaacg aattttctcc cagcggcccg tgctgatcga gcgagaccag ggaaacacca 27420
cggtttccat ctactgcatt tgtaatcacc ccggattgca tgaaagcctt tgctgtctta 27480
tgtgtactga gtttaataaa aactgaatta agactctcct acggactgcc gcttcttcaa 27540
cccggatttt acaaccagaa gaacgaaact tttcctgtcg tccaggactc tgttaacttc 27600
acctttccta ctcacaaact agaagctcaa cgactacacc gcttttccag aagcattttc 27660
cctactaata ctactttcaa aaccggaggt gagctccaag gtcttcctac agaaaaccct 27720
tgggtggaag cgggccttgt agtgctagga attcttgcgg gtgggcttgt gattattctt 27780
tgctacctat acacaccttg cttcactttc ctagtggtgt tgtggtattg gtttaaaaaa 27840
tggggcccat actagtcttg cttgttttac tttcgctttt ggaaccgggt tctgccaatt 27900
acgatccatg tctagacttc gacccagaaa actgcacact tacttttgca cccgacacaa 27960
gccgcatctg tggagttctt attaagtgcg gatgggaatg caggtccgtt gaaattacac 28020
acaataacaa aacctggaac aataccttat ccaccacatg ggagccagga gttcccgagt 28080
ggtacactgt ctctgtccga ggtcctgacg gttccatccg cattagtaac aacactttca 28140
ttttttctga aatgtgcgat ctggccatgt tcatgagcaa acagtattct ctatggcctc 28200
ctagcaagga caacatcgta acgttctcca ttgcttattg cttgtgcgct tgccttctta 28260
ctgctttact gtgcgtatgc atacacctgc ttgtaaccac tcgcatcaaa aacgccaata 28320
acaaagaaaa aatgccttaa cctctttctg tttacagaca tggcttctct tacatctctc 28380
atatttgtca gcattgtcac tgccgctcac ggacaaacag tcgtctctat ccctctagga 28440
cataattaca ctctcatagg acccccaatc acttcagagg tcatctggac caaactggga 28500
agcgttgatt actttgatat aatctgcaac aaaacaaaac caataatagt aacttgcaac 28560
atacaaaatc ttacattgat taatgttagc aaagtttaca gcggttacta ttatggttat 28620
gacagataca gtagtcaata tagaaattac ttggttcgtg ttacccagtt aaaaaccacg 28680
aaaatgccaa atatggcaaa gattcgatcc gatgacaatt ctctagaaac ttttacatct 28740
cccaccacac ccgacgaaaa aaacatccca gattcaatga ttgcaattgt tgcagcggtg 28800
gcagtggtga tggcactaat aataatatgc atgcttttat atgcttgtcg ctacaaaaag 28860
tttcatccta aaaaacaaga tctcctacta aggcttaaca tttaatttct ttttatacag 28920
ccatggtttc cactaccaca ttccttatgc ttactagtct tgcaactctg acttctgctc 28980
gctcacacct cactgtaact ataggctcaa actgcacact aaaaggacct caaggtggtc 29040
atgtcttttg gtggagaata tatgacaatg gatggtttac aaaaccatgt gaccaacctg 29100
gtagattttt ctgcaacggc agagacctaa ccattatcaa cgtgacagca aatgacaaag 29160
gcttctatta tggaaccgac tataaaagta gtttagatta taacattatt gtactgccat 29220
ctaccactcc agcaccccgc acaactactt tctctagcag cagtgtcgct aacaatacaa 29280
tttccaatcc aacctttgcc gcgcttttaa aacgcactgt gaataattct acaacttcac 29340
atacaacaat ttccacttca acaatcagca ttatcgctgc agtgacaatt ggaatatcta 29400
ttcttgtttt taccataacc tactacgcct gctgctatag aaaagacaaa cataaaggtg 29460
atccattact tagatttgat atttaatttg ttcttttttt ttttatttac agtatggtga 29520
acaccaatca tggtacctag aaatttcttc ttcaccatac tcatttgtgc atttaatgtt 29580
tgcgctactt tcacagcagt agccacagca accccagact gtataggagc atttgcttcc 29640
tatgcacttt ttgcttttgt tacttgcatc tgcgtatgta gcatagtctg cctggttatt 29700
aattttttcc aacttctaga ctggatcctt gtgcgaattg cctacctgcg ccaccatccc 29760
gaataccgca accaaaatat cgcggcactt cttagactca tctaaaacca tgcaggctat 29820
actaccaata tttttgcttc tattgcttcc ctacgctgtc tcaaccccag ctgcctatag 29880
tactccacca gaacacctta gaaaatgcaa attccaacaa ccgtggtcat ttcttgcttg 29940
ctatcgagaa aaatcagaaa ttcccccaaa tttaataatg attgctggaa taattaatat 30000
aatctgttgc accataattt catttttgat atacccccta tttgattttg gctggaatgc 30060
tcccaatgca catgatcatc cacaagaccc agaggaacac attcccctac aaaacatgca 30120
acatccaata gcgctaatag attacgaaag tgaaccacaa cccccactac tccctgctat 30180
tagttacttc aacctaaccg gcggagatga ctgaaacact caccacctcc aattccgccg 30240
aggatctgct cgatatggac ggccgcgtct cagaacagcg acttgcccaa ctacgcatcc 30300
gccagcagca ggaacgcgcg gccaaagagc tcagagatgt catccaaatt caccaatgca 30360
aaaaaggcat attctgtttg gtaaaacaag ccaagatatc ctacgagatc accgctactg 30420
accatcgcct ctcttacgaa cttggccccc aacgacaaaa atttacctgc atggtgggaa 30480
tcaaccccat agttatcacc cagcaaagtg gagatactaa gggttgcatt cactgctcct 30540
gcgattccat cgagtgcacc tacaccctgc tgaagaccct atgcggccta agagacctgc 30600
taccaatgaa tggaagcgga gctactaact tcagcctgct gaagcaggct ggtgacgtcg 30660
aggagaatcc tggcccaatg gtgagcaaag gcgaagagct gttcaccggc gtggtgccca 30720
tcctggtgga gctggacggc gacgtgaacg gccacaagtt cagcgtgagc ggcgagggcg 30780
agggcgacgc cacctacggc aagctgaccc tgaagctgct gtgcaccacc ggcaagctgc 30840
ccgtgccctg gcccaccctg gtgaccaccc tgggctacgg cgtgcagtgc ttcgcccggt 30900
accccgacca catgaagcag cacgacttct tcaagagcgc catgcccgag ggctacgtgc 30960
aggagcggac catcttcttc aaggacgacg gcaactacaa gacccgggcc gaggtgaagt 31020
tcgagggcga caccctggtg aaccggatcg agctgaaggg catcgacttc aaggaggacg 31080
gcaacatcct gggccacaag ctggagtaca actacaacag ccacaacgtg tacatcaccg 31140
ccgacaagca gaagaacggc atcaaggcca acttcaagat ccggcacaac atcgaggacg 31200
gcggcgtgca gctggccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 31260
tgctgcccga caaccactac ctgagctacc agagcgccct gttcaaggac cccaacgaga 31320
agcgggacca catggtgctg ctggagttcc tgaccgccgc cggcatcacc gagggcatga 31380
acgagctcta taagtaaaaa atgattaata aaaaatcact tacttgaaat cagcaataag 31440
gtctctgttg aaattttctc ccagcagcac ctcacttccc tcttcccaac tctggtattc 31500
taaaccccgt tcagcggcat actttctcca tactttaaag gggatgtcaa attttagctc 31560
ctctcctgta cccacaatct tcatgtcttt cttcccagat gaccaagaga gtccggctca 31620
gtgactcctt caaccctgtc tacccctatg aagatgaaag cacctcccaa caccccttta 31680
taaacccagg gtttatttcc ccaaatggct tcacacaaag cccagacgga gttcttactt 31740
taaaatgttt aaccccacta acaaccacag gcggatctct acagctaaaa gtgggagggg 31800
gacttacagt ggatgacact gatggtacct tacaagaaaa catacgtgct acagcaccca 31860
ttactaaaaa taatcactct gtagaactat ccattggaaa tggattagaa actcaaaaca 31920
ataaactatg tgccaaattg ggaaatgggt taaaatttaa caacggtgac atttgtataa 31980
aggatagtat taacacctta tggactggaa taaaccctcc acctaactgt caaattgtgg 32040
aaaacactaa tacaaatgat ggcaaactta ctttagtatt agtaaaaaac ggagggcttg 32100
ttaatggcta cgtgtctcta gttggtgtat cagacactgt gaaccaaatg ttcacacaaa 32160
agacagcaaa catccaatta agattatatt ttgactcttc tggaaatcta ttaactgatg 32220
aatcagactt aaaaattcca cttaaaaata aatcttctac agcgaccagt gaaactgtag 32280
ccagcagcaa agcctttatg ccaagtacta cagcttatcc cttcaacacc actactaggg 32340
atagtgaaaa ctacattcat ggaatatgtt actacatgac tagttatgat agaagtctat 32400
ttcccttgaa catttctata atgctaaaca gccgtatgat ttcttccaat gttgcctatg 32460
ccatacaatt tgaatggaat ctaaatgcaa gtgaatctcc agaaagcaac atagctacgc 32520
tgaccacatc cccctttttc ttttcttaca ttacagaaga cgacaactaa aataaagttt 32580
aagtgttttt atttaaaact cacaaaattc gagtagttat tttgcctcca ccttcccatt 32640
tgacagaata caccaatctc tccccacgca cagctttaaa catttggata ccattagaga 32700
tagacattgt tttagattcc acattccaaa cagtttcaga gcgagccaat ctggggtcag 32760
tgatagataa aaatccatcg cgatagtctt ttaaagcgct ttcacagtcc aactgctgcg 32820
gatgcgaatc cggagtctgg atcacggtca tctggaagaa gaacgatggg aatcataatc 32880
cgaaaacggt atcggacgat tgtgtctcat caaacccaca agcagccgct gtctgcgtcg 32940
ctccgtgcaa ctgctgttta tgggatcagg gtccacagtg tcctgaagca tgattttaat 33000
agcccttaac atcaactttc tggtgcgatg cgcgcagcaa cgcattctga tttcactcaa 33060
atctttgcag taggtacaac acattattac aatattgttt aataaaccat aattaaaagc 33120
gctccagcca aaactcatat ctgatataat cgcccctgca tgaccatcat accaaagttt 33180
aatataaatt aaatgacgtt ccctcaaaaa cacactaccc acatacatga tctcttttgg 33240
catgtgcata ttaacaatct gtctgtacca tggacaacgt tggttaatca tgcaacccaa 33300
tataaccttc cggaaccaca ctgccaacac cgctccccca gccatgcatt gaagtgaacc 33360
ctgctgatta caatgacaat gaagaaccca attctctcga ccgtgaatca cttgagaatg 33420
aaaaatatct atagtggcac aacatagaca taaatgcatg catcttctca taatttttaa 33480
ctcctcagga tttagaaaca tatcccaggg aataggaagc tcttgcagaa cagtaaagct 33540
ggcagaacaa ggaagaccac gaacacaact tacactatgc atagtcatag tatcacaatc 33600
tggcaacagc gggtggtctt cagtcataga agctcgggtt tcattttcct cacaacgtgg 33660
taactgggct ctggtgtaag ggtgatgtct ggcgcatgat gtcgagcgtg cgcgcaacct 33720
tgtcataatg gagttgcttc ctgacattct cgtattttgt atagcaaaac gcggccctgg 33780
cagaacacac tcttcttcgc cttctatcct gccgcttagc gtgttccgtg tgatagttca 33840
agtacagcca cactcttaag ttggtcaaaa gaatgctggc ttcagttgta atcaaaactc 33900
catcgcatct aattgttctg aggaaatcat ccacggtagc atatgcaaat cccaaccaag 33960
caatgcaact ggattgcgtt tcaagcagga gaggagaggg aagagacgga agaaccatgt 34020
taatttttat tccaaacgat ctcgcagtac ttcaaattgt agatcgcgca gatggcatct 34080
ctcgccccca ctgtgttggt gaaaaagcac agctaaatca aaagaaatgc gattttcaag 34140
gtgctcaacg gtggcttcca acaaagcctc cacgcgcaca tccaagaaca aaagaatacc 34200
aaaagaagga gcattttcta actcctcaat catcatatta cattcctgca ccattcccag 34260
ataattttca gctttccagc cttgaattat tcgtgtcagt tcttgtggta aatccaatcc 34320
acacattaca aacaggtccc ggagggcgcc ctccaccacc attcttaaac acaccctcat 34380
aatgacaaaa tatcttgctc ctgtgtcacc tgtagcgaat tgagaatggc aacatcaatt 34440
gacatgccct tggctctaag ttcttcttta agttctagtt gtaaaaactc tctcatatta 34500
tcaccaaact gcttagccag aagccccccg ggaacaagag caggggacgc tacagtgcag 34560
tacaagcgca gacctcccca attggctcca gcaaaaacaa gattggaata agcatattgg 34620
gaaccgccag taatatcatc gaagttgctg gaaatataat caggcagagt ttcttgtaaa 34680
aattgaataa aagaaaaatt tgccaaaaaa acattcaaaa cctctgggat gcaaatgcaa 34740
taggttaccg cgctgcgctc caacattgtt agttttgaat tagtctgcaa aaataaaaaa 34800
aaaaacaagc gtcatatcat agtagcctga cgaacaggtg gataaatcag tctttccatc 34860
acaagacaag ccacagggtc tccagctcga ccctcgtaaa acctgtcatg gtgattaaac 34920
aacagcaccg aaagttcctc gcggtgacca gcatgaataa ttcttgatga agcatacaat 34980
ccagacatgt tagcatcagt taacgagaaa aaacagccaa catagccttt gggtataatt 35040
atgcttaatc gtaagtatag caaagccacc cctcgcggat acaaagtaaa aggcacagga 35100
gaataaaaaa tataattatt tctctgctgc tgttcaggca acgtcgcccc cggtccctct 35160
aaatacacat acaaagcctc atcagccatg gcttaccaga caaagtacag cgggcacgca 35220
caagctctaa agtcactctc caacctctcc acaatatata tacacaagcc ctaaactgac 35280
gtaatgggag taaagtgtaa aaaatcccgc caaacccaac acacaccccg aaactgcgtc 35340
accagggaaa agtacagttt cacttccgca atcccaacaa gcgtcacttc ctctttctca 35400
cggtacgtca catcccatta acttgcaacg tcattttccc acggccgcgc cgccccgttt 35460
agccgttaac cccacagcca atcaccacac accccacaat ttttaaaatc acctcattta 35520
catattggca ccattccatc tataaggtat attattgatg atg 35563

Claims (20)

1.重组腺病毒基因组，包括异源开放阅读框(ORF)和自切割肽编码序列，两者均可操作地连接至与内源腺病毒ORF相同的阅读框中且在所述相同的阅读框中，其中所述自切割肽编码序列位于所述异源ORF和所述内源ORF之间，且其中：
所述内源ORF是E1B-55k且所述异源ORF在E1B-55k的3'；
所述内源ORF是DNA聚合酶且所述异源ORF在DNA聚合酶的5'；
所述内源ORF是DNA结合蛋白(DBP)且所述异源ORF在DBP的3'；
所述内源ORF是腺病毒死亡蛋白(ADP)且所述异源ORF在ADP的5'；
所述内源ORF是E3-14.7k且所述异源ORF在E3-14.7k的3'；
所述内源ORF是E4-ORF2且所述异源ORF在E4-ORF2的5'；或者
所述内源ORF是纤维蛋白且所述异源ORF在纤维蛋白的3'；
其中所述异源ORF编码治疗性蛋白。
2.如权利要求1所述的重组腺病毒基因组，其中所述治疗性蛋白包括免疫调节剂。
3.如权利要求1或权利要求2所述的重组腺病毒基因组，其中所述自切割肽是2A肽或其变体。
4.如权利要求3所述的重组腺病毒基因组，其中所述2A肽包括猪捷申病毒-1(PTV1)2A(P2A)肽、口蹄疫病毒(FMDV)2A(F2A)肽、马鼻炎A病毒(ERAV)2A(E2A)肽或明脉扁刺蛾β四体病毒(TaV)2A(T2A)肽，或其变体。
5.如权利要求4所述的重组腺病毒基因组，其中所述自切割肽的氨基酸序列与SEQ IDNO:14-21中的任何一个的氨基酸序列至少80％、至少85％、至少90％或至少95％一致。
6.如权利要求4所述的重组腺病毒基因组，其中所述自切割肽包括所述SEQ ID NO:14-21中的任何一个的氨基酸序列。
7.包括如权利要求1-6中任一项所述的重组腺病毒基因组和药学上可接受的载体的组合物。
8.包括如权利要求1-6中任一项所述的重组腺病毒基因组的重组腺病毒。
9.包括如权利要求8所述的重组腺病毒和药学上可接受的载体的组合物。
10.将治疗性蛋白递送至受试者的方法，包括向所述受试者施用如权利要求1-6中任一项所述的重组腺病毒基因组、如权利要求8所述的重组腺病毒、或如权利要求7或权利要求9所述的组合物。
11.抑制肿瘤细胞活力和/或肿瘤细胞生长的方法，包括将所述肿瘤细胞与如权利要求1-6中任一项所述的重组腺病毒基因组、如权利要求8所述的重组腺病毒、或如权利要求7或权利要求9所述的组合物相接触。
12.如权利要求11所述的方法，其中所述方法为体外方法。
13.如权利要求11所述的方法，其中所述方法为体内方法且接触所述肿瘤细胞包括向患有肿瘤的受试者施用所述重组腺病毒基因组、重组腺病毒或组合物。
14.抑制受试者中肿瘤进展或减小肿瘤体积的方法，包括向所述受试者施用治疗有效量的如权利要求1-6中任一项所述的重组腺病毒基因组、如权利要求8所述的重组腺病毒、或如权利要求7或权利要求9所述的组合物，从而抑制受试者中肿瘤进展或减小肿瘤体积。
15.治疗受试者中癌症的方法，包括向所述受试者施用治疗有效量的如权利要求1-6中任一项所述的重组腺病毒基因组、如权利要求8所述的重组腺病毒、或如权利要求7或权利要求9所述的组合物，从而治疗受试者中的癌症。
16.如权利要求13-15中任一项所述的方法，进一步包括向所述受试者施用额外的治疗剂。
17.试剂盒，包括：
(i)如权利要求1-6中任一项所述的重组腺病毒基因组、如权利要求8所述的重组腺病毒、或如权利要求7或权利要求9所述的组合物；以及
(ii)一种或更多种额外的治疗剂和/或一种或更多种诊断剂。
18.如权利要求17所述的试剂盒，其中所述一种或更多种额外的治疗剂包括化疗剂、生物剂或其组合。
19.如权利要求17或18所述的试剂盒，其中所述一种或更多种诊断剂包括一种或更多种对肿瘤标志物特异的抗体。
20.如权利要求17或18所述的试剂盒，其中所述一种或更多种诊断剂包括一种或更多种对肿瘤标志物特异的核酸分子。
CN201780013061.3A 2016-02-23 2017-02-23 对病毒动力学影响最小的治疗性腺病毒中的外源基因表达 Active CN108699566B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310696656.0A CN117384961A (zh) 2016-02-23 2017-02-23 对病毒动力学影响最小的治疗性腺病毒中的外源基因表达

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662298653P 2016-02-23 2016-02-23
US62/298,653 2016-02-23
PCT/US2017/019086 WO2017147269A1 (en) 2016-02-23 2017-02-23 Exogenous gene expression in therapeutic adenovirus for minimal impact on viral kinetics

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202310696656.0A Division CN117384961A (zh) 2016-02-23 2017-02-23 对病毒动力学影响最小的治疗性腺病毒中的外源基因表达

Publications (2)

Publication Number Publication Date
CN108699566A true CN108699566A (zh) 2018-10-23
CN108699566B CN108699566B (zh) 2023-06-30

Family

ID=58358815

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202310696656.0A Pending CN117384961A (zh) 2016-02-23 2017-02-23 对病毒动力学影响最小的治疗性腺病毒中的外源基因表达
CN201780013061.3A Active CN108699566B (zh) 2016-02-23 2017-02-23 对病毒动力学影响最小的治疗性腺病毒中的外源基因表达

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CN202310696656.0A Pending CN117384961A (zh) 2016-02-23 2017-02-23 对病毒动力学影响最小的治疗性腺病毒中的外源基因表达

Country Status (9)

Country Link
US (3) US10738325B2 (zh)
EP (2) EP4155411A1 (zh)
JP (2) JP7015551B2 (zh)
KR (3) KR102471633B1 (zh)
CN (2) CN117384961A (zh)
AU (2) AU2017223589B2 (zh)
CA (1) CA3013639A1 (zh)
ES (1) ES2933174T3 (zh)
WO (1) WO2017147269A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109758467A (zh) * 2019-03-08 2019-05-17 中国农业科学院兰州兽医研究所 一种吉西他滨在制备预防口蹄疫病毒感染的药物中的应用

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2903582C (en) 2013-03-14 2021-06-08 Salk Institute For Biological Studies Oncolytic adenovirus compositions
AU2016333996B2 (en) 2015-10-05 2020-05-28 Salk Institute For Biological Studies Synthetic adenoviruses with tropism to damaged tissue for use in promoting wound repair and tissue regeneration
EP4155411A1 (en) * 2016-02-23 2023-03-29 Salk Institute for Biological Studies Exogenous gene expression in therapeutic adenovirus for minimal impact on viral kinetics
JP7054527B2 (ja) 2016-02-23 2022-04-14 ソーク インスティテュート フォー バイオロジカル スタディーズ アデノウイルスの複製動態を測定するための高スループットアッセイ
WO2018111767A1 (en) 2016-12-12 2018-06-21 Salk Institute For Biological Studies Tumor-targeting synthetic adenoviruses and uses thereof
MX2020010499A (es) * 2018-04-09 2020-10-28 Salk Inst For Biological Studi Composiciones de adenovirus oncoliticas con propiedades aumentadas de replicacion.
CA3188762A1 (en) * 2020-07-06 2022-01-13 Salk Institute For Biological Studies Recombinant adenovirus genome having a synthetic transcriptional unit and two step transcriptional regulation and amplification
CN112592388A (zh) * 2020-12-14 2021-04-02 河南普诺易生物制品研究院有限公司 一种2a肽、双顺反子表达载体、重组蛋白表达系统及应用
IL305175A (en) * 2021-02-19 2023-10-01 Angeles Therapeutics Inc Single-chain and multi-chain synthetic antigen receptors for diverse immune cells
KR20240099339A (ko) * 2021-10-25 2024-06-28 젠비보, 인크. 치료제 전달을 위한 조성물 및 방법

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014000026A1 (en) * 2012-06-25 2014-01-03 University Of Canberra Recombinant viral vectors and uses therefor
WO2015155370A1 (en) * 2014-04-12 2015-10-15 Psioxus Therapeutics Limited Group b adenovirus modified in the e4orf4 region
WO2016049201A1 (en) * 2014-09-24 2016-03-31 Salk Institute For Biological Studies Oncolytic tumor viruses and methods of use

Family Cites Families (330)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5670488A (en) 1992-12-03 1997-09-23 Genzyme Corporation Adenovirus vector for gene therapy
US5747469A (en) 1991-03-06 1998-05-05 Board Of Regents, The University Of Texas System Methods and compositions comprising DNA damaging agents and p53
US6410010B1 (en) 1992-10-13 2002-06-25 Board Of Regents, The University Of Texas System Recombinant P53 adenovirus compositions
FR2688514A1 (fr) 1992-03-16 1993-09-17 Centre Nat Rech Scient Adenovirus recombinants defectifs exprimant des cytokines et medicaments antitumoraux les contenant.
US5846945A (en) 1993-02-16 1998-12-08 Onyx Pharmaceuticals, Inc. Cytopathic viruses for therapy and prophylaxis of neoplasia
DK0931830T3 (da) 1993-02-16 2001-06-11 Onyx Pharma Inc Cytopatiske vira til terapi og profylakse af neoplasi
US7045347B2 (en) 1993-06-24 2006-05-16 Advec, Inc. Helper dependent adenovirus vectors based on integrase family site-specific recombinases
US20010006629A1 (en) 1993-10-25 2001-07-05 Richard J. Gregory Recombinant adenoviral vector and methods of use
US7252989B1 (en) 1994-04-04 2007-08-07 Board Of Regents, The University Of Texas System Adenovirus supervector system
US5965541A (en) 1995-11-28 1999-10-12 Genvec, Inc. Vectors and methods for gene transfer to cells
US5962311A (en) 1994-09-08 1999-10-05 Genvec, Inc. Short-shafted adenoviral fiber and its use
US5846782A (en) 1995-11-28 1998-12-08 Genvec, Inc. Targeting adenovirus with use of constrained peptide motifs
US5559099A (en) 1994-09-08 1996-09-24 Genvec, Inc. Penton base protein and methods of using same
US6465253B1 (en) 1994-09-08 2002-10-15 Genvec, Inc. Vectors and methods for gene transfer to cells
FR2726285B1 (fr) 1994-10-28 1996-11-29 Centre Nat Rech Scient Adenovirus depourvus de particules contaminantes viables, preparation et utilisation
AU709498B2 (en) 1994-12-12 1999-09-02 Genetic Therapy, Inc. Improved adenoviral vectors and producer cells
US5770442A (en) 1995-02-21 1998-06-23 Cornell Research Foundation, Inc. Chimeric adenoviral fiber protein and methods of using same
US6127525A (en) 1995-02-21 2000-10-03 Cornell Research Foundation, Inc. Chimeric adenoviral coat protein and methods of using same
US20030026789A1 (en) 1995-05-03 2003-02-06 Richard J. Gregory Gene therapy using replication competent targeted adenoviral vectors
US6506379B1 (en) 1995-06-07 2003-01-14 Ariad Gene Therapeutics, Inc. Intramuscular delivery of recombinant AAV
AUPN477695A0 (en) 1995-08-14 1995-09-07 Commonwealth Scientific And Industrial Research Organisation Gene therapy
US5945335A (en) 1995-11-09 1999-08-31 Avigen, Inc. Adenovirus helper-free system for producing recombinant AAV virions lacking oncogenic sequences
EP0861329B1 (fr) 1995-11-13 2005-03-16 Commissariat A L'energie Atomique Complexe proteique dodecaedrique adenoviral, composition le contenant et ses applications
IL124654A0 (en) 1995-11-28 1998-12-06 Genvec Inc Vectors and method for gene transfer to cells
US6133243A (en) 1996-02-22 2000-10-17 Onyx Pharmaceuticals, Inc. Liposomal-viral DNA complexes for treating disease
FR2746110B1 (fr) 1996-03-14 1998-04-17 Methode de traitement par therapie genique des tumeurs humaines et virus recombinants correspondants
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6485958B2 (en) 1996-07-01 2002-11-26 Gencell Sa Method for producing recombinant adenovirus
US7232899B2 (en) 1996-09-25 2007-06-19 The Scripps Research Institute Adenovirus vectors, packaging cell lines, compositions, and methods for preparation and use
US5922315A (en) 1997-01-24 1999-07-13 Genetic Therapy, Inc. Adenoviruses having altered hexon proteins
US6403370B1 (en) 1997-02-10 2002-06-11 Genstar Therapeutics Corporation Oncolytic/immunogenic complementary-adenoviral vector system
US20050287120A1 (en) 1997-03-21 2005-12-29 Fisher Paul B Cancer - targeted viral vectors
FR2761689B1 (fr) 1997-04-02 1999-06-25 Transgene Sa Fibre adenovirale modifiee et adenovirus cibles
EP0988390A1 (en) 1997-05-28 2000-03-29 Genvec, Inc. Alternatively targeted adenovirus
US6200799B1 (en) 1997-06-03 2001-03-13 University Of Lausanne Somatic gene therapy to suppress secondary cataract formation following eye surgery
EP0996735B1 (en) 1997-06-09 2003-12-10 Genvec, Inc. Chimeric vectors comprising a phage packaging site and a portion derived from the genome of a eukaryotic virus
WO1999031261A1 (en) 1997-12-12 1999-06-24 Onyx Pharmaceuticals, Inc. SELECTIVE KILLING AND DIAGNOSIS OF p53+ NEOPLASTIC CELLS
WO1999039734A1 (en) 1998-02-06 1999-08-12 The Uab Research Foundation Adenovirus vector containing a heterologous peptide epitope in the hi loop of the fiber knob
US6984635B1 (en) 1998-02-13 2006-01-10 Board Of Trustees Of The Leland Stanford Jr. University Dimerizing agents, their production and use
KR20010074429A (ko) 1998-02-17 2001-08-04 피터 브이. 오`네일 섬유 교체 단백질을 함유하는 변형된 아데노바이러스
US20050095231A1 (en) 1998-02-17 2005-05-05 Curiel David T. Modified adenovirus containing a fiber replacement protein
WO1999044423A1 (en) 1998-03-03 1999-09-10 The Uab Research Foundation Amplification of gene transfer and gene therapy by controlled replication
AU3358999A (en) 1998-03-20 1999-10-11 Genzyme Corporation Chimeric adenoviral vectors for targeted gene delivery
US6670188B1 (en) 1998-04-24 2003-12-30 Crucell Holland B.V. Packaging systems for human recombinant adenovirus to be used in gene therapy
US7829329B2 (en) 1998-04-24 2010-11-09 Onyx Pharmaceuticals, Inc. Adenoviral vectors for treating disease
ATE258228T1 (de) 1998-07-07 2004-02-15 Transgene Sa Verwendung der leserahmen der adenoviralen e4- region zur verbesserung der genexpression
US20030017138A1 (en) 1998-07-08 2003-01-23 Menzo Havenga Chimeric adenoviruses
AU773202B2 (en) 1998-08-27 2004-05-20 Aventis Pharma S.A. Targeted adenovirus vectors for delivery of heterologous genes
US6900049B2 (en) 1998-09-10 2005-05-31 Cell Genesys, Inc. Adenovirus vectors containing cell status-specific response elements and methods of use thereof
EP1112372A1 (en) 1998-09-11 2001-07-04 Genvec, Inc. Alternatively targeted adenovirus
US6841540B1 (en) 1998-09-29 2005-01-11 The Uab Research Foundation Immunomodulation by genetic modification of dendritic cells and B cells
JP4404490B2 (ja) 1998-10-15 2010-01-27 カンジ，インコーポレイテッド 選択的に複製するウイルスベクター
US20020037274A1 (en) 1998-10-26 2002-03-28 Angelica Williams Single agent method for killing tumor and tumor associated endothelial cells using adenoviral mutants
US6929946B1 (en) 1998-11-20 2005-08-16 Crucell Holland B.V. Gene delivery vectors provided with a tissue tropism for smooth muscle cells, and/or endothelial cells
CA2359795A1 (en) 1999-01-14 2000-07-20 Novartis Ag Adenovirus vectors, packaging cell lines, compositions, and methods for preparation and use
US6395875B1 (en) 1999-01-25 2002-05-28 Brookhaven Science Associates Llc Recombinant soluble adenovirus receptor
US7157266B2 (en) 1999-01-25 2007-01-02 Brookhaven Science Associates Llc Structure of adenovirus bound to cellular receptor car
JP4683727B2 (ja) 1999-03-04 2011-05-18 クルーセル ホランド ベスローテン フェンノートシャップ 線維芽細胞様またはマクロファージ様細胞の形質導入のための手段と方法
US6869936B1 (en) 1999-03-04 2005-03-22 Crucell Holland B.V. Means and methods for fibroblast-like or macrophage-like cell transduction
AU767904B2 (en) 1999-05-12 2003-11-27 Uab Research Foundation, The Infectivity-enhanced conditionally-replicative adenovirus and uses thereof
US20040175362A1 (en) 1999-05-12 2004-09-09 Curiel David T. Infectivity-enhanced conditionally-replicative adenovirus and uses thereof
KR100741247B1 (ko) 1999-05-17 2007-07-19 크루셀 홀란드 비.브이. 아데노바이러스 타입 35의 적어도 하나의 구성성분을포함하는 아데노바이러스 유래 유전자 전달체
US20050232900A1 (en) 1999-05-18 2005-10-20 Crucell Holland B.V. Serotype of adenovirus and uses thereof
US6913922B1 (en) 1999-05-18 2005-07-05 Crucell Holland B.V. Serotype of adenovirus and uses thereof
WO2000073478A2 (en) 1999-06-01 2000-12-07 University Of Washington Recombinant adenoviral vectors expressing chimeric fiber proteins for cell specific infection and genome integration
US7094398B1 (en) 1999-06-01 2006-08-22 University Of Washington Recombinant adenoviral vectors expressing chimeric fiber proteins for cell specific infection and genome integration
EP1593742A3 (en) 1999-06-01 2006-02-01 The University of Washington Recombinant adenoviral vectors expressing chimeric fiber proteins for cell specific infection
FR2794771B1 (fr) 1999-06-11 2001-08-10 Aventis Pharma Sa Adenovirus recombinants codant pour le transporteur specifique de l'iode (nis)
DE19929569A1 (de) 1999-06-21 2000-12-28 Holm Per Sonne Mittel zur Behandlung maligner Erkrankungen unter Verwendung des Proteins YB-1
JP2003531568A (ja) 1999-07-06 2003-10-28 ゴット−エー−ジェーン・エービー 組換え型アデノウイルス
US7589069B1 (en) 1999-07-12 2009-09-15 Saint Louis University Replication-competent anti-cancer vectors
EP1124977A1 (fr) 1999-08-27 2001-08-22 Transgene S.A. Fibre adenovirale modifiee et utilisations
WO2001021217A2 (en) 1999-09-23 2001-03-29 Genvec, Inc. Method of treating cells of the prostate prophylactically or therapeutically
US6555368B1 (en) 1999-09-24 2003-04-29 Uab Research Foundation Capsid-modified recombinant adenovirus and methods of use
WO2001023004A1 (en) 1999-09-30 2001-04-05 The Trustees Of The University Of Pennsylvania Replication selective adenoviruses for use in cancer therapy
US6635476B1 (en) 1999-10-15 2003-10-21 Canji, Inc. Targeted vectors
MXPA02004736A (es) 1999-11-12 2003-01-28 Oncolytics Biotech Inc Virus para el tratamiento de trastornos proliferativos celulares.
ES2288488T3 (es) 1999-11-15 2008-01-16 Onyx Pharmaceuticals, Inc. Adenovirus oncoliticos.
US6867022B1 (en) 2000-01-21 2005-03-15 Regents Of The University Of Michigan Replication deficient adenovirus vectors and methods of making and using them
WO2001058940A2 (en) 2000-02-09 2001-08-16 Genvec, Inc. Adenoviral capsid containing chimeric protein ix
US6911200B2 (en) 2000-03-24 2005-06-28 Cell Genesys, Inc. Methods of treating neoplasia with combination of target-cell specific adenovirus, chemotherapy and radiation
US6692736B2 (en) 2000-03-24 2004-02-17 Cell Genesys, Inc. Cell-specific adenovirus vectors comprising an internal ribosome entry site
WO2001073093A2 (en) 2000-03-24 2001-10-04 Cell Genesys, Inc. Cell-specific adenovirus vectors comprising an internal ribosome entry site
US20030082146A1 (en) 2000-04-26 2003-05-01 Van Es Helmuth H. G. Adenovirus vectors with knobless fibers, and their uses
JP2003531609A (ja) 2000-05-01 2003-10-28 ノバルティス アクチエンゲゼルシャフト 目に関する形質導入用ベクターおよび遺伝子治療を目的とするその用途
US7332337B2 (en) 2000-05-16 2008-02-19 Galapagos Nv Viral vectors having tissue tropism for T-lymphocytes, B- and mast cells
EP1157999A1 (en) 2000-05-24 2001-11-28 Introgene B.V. Methods and means for enhancing skin transplantation using gene delivery vehicles having tropism for primary fibroblasts, as well as other uses thereof
EP1284294A4 (en) 2000-05-26 2004-10-13 Sumitomo Pharma NOVEL RECOMBINANT ADENOVIRUS VECTOR WITH REDUCED SIDE EFFECTS
JP2003534806A (ja) 2000-05-31 2003-11-25 ジェンベク、インコーポレイティッド アデノウイルスベクターのターゲティングの方法および組成物
CA2410665C (en) 2000-05-31 2017-11-07 University Of Saskatchewan Modified bovine adenovirus having altered tropism
US7754201B2 (en) 2000-06-02 2010-07-13 GenPhar, Inc Method of vaccination through serotype rotation
EP1167533A1 (en) 2000-06-23 2002-01-02 Vereniging Voor Christelijk Wetenschappelijk Onderwijs Methods and means for the complementation of viral protein expression in stable cell lines
US6579522B1 (en) 2000-06-27 2003-06-17 Genvec, Inc. Replication deficient adenoviral TNF vector
US7022496B2 (en) 2000-07-14 2006-04-04 The Johns Hopkins University Use of gene product of adenovirus early region 4 ORF-6 to inhibit repair of double-strand breaks in DNA
US20020106382A1 (en) 2000-07-14 2002-08-08 Young Charles S.H. Modified adenovirus and uses thereof
GB0017720D0 (en) 2000-07-19 2000-09-06 Got A Gene Ab Modified virus
AU9434801A (en) 2000-08-10 2002-02-18 Crucell Holland Bv Gene delivery vectors with cell type specificity for primary human chondrocytes
US7235233B2 (en) 2000-09-26 2007-06-26 Crucell Holland B.V. Serotype 5 adenoviral vectors with chimeric fibers for gene delivery in skeletal muscle cells or myoblasts
JP4394878B2 (ja) 2000-12-08 2010-01-06 ライフ テクノロジーズ コーポレーション 複数の認識部位を用いた核酸分子合成のための方法および組成物
US20030095989A1 (en) 2000-12-18 2003-05-22 Irving John M. Chimeric cytolytic viruses for cancer treatment
US6635466B2 (en) 2001-01-09 2003-10-21 University Of Iowa Research Foundation Adenovirus serotype 30 (Ad30)
US20020168343A1 (en) 2001-02-14 2002-11-14 Curiel David T. Combined transductional and transcriptional targeting system for improved gene delivery
IL152420A0 (en) 2001-02-23 2003-05-29 Novartis Ag Novel oncolytic adenoviral vectors
AU2002244757A1 (en) 2001-04-02 2002-10-15 Bayer Aktiengesellschaft Method for specifically detecting, isolating and characterizing cells from body samples by transfecting nucleic acid constructs
US20030219899A1 (en) 2001-04-17 2003-11-27 Nikolay Korokhov Mosaic adenoviral vectors
EP1390476A4 (en) 2001-04-17 2005-03-30 Vectorlogics Inc MOSAIC adenovirus vectors
US20030138405A1 (en) 2001-04-17 2003-07-24 Juan Fueyo Conditionally replicative adenovirus to target the Rb and Rb-related pathways
ATE530672T1 (de) 2001-06-22 2011-11-15 Univ Pennsylvania Rekombinante adenoviren mit affen-adenovirus proteinen und verwendung davon.
US6844192B2 (en) 2001-06-29 2005-01-18 Wake Forest University Adenovirus E4 protein variants for virus production
US6905678B2 (en) 2001-07-07 2005-06-14 Crucell Holland B.V. Gene delivery vectors with cell type specificity for mesenchymal stem cells
AU2002346084B2 (en) 2001-07-23 2006-11-16 Onyx Pharmaceuticals, Inc. Viral mutants that selectively replicate in targeted human cancer cells
CA2460157A1 (en) 2001-09-18 2003-03-27 Clontech Laboratories, Inc. Site-specific recombinase based method for producing adenoviral vectors
US7569217B2 (en) 2001-09-24 2009-08-04 University Of Saskatchewan Porcine adenovirus E1 and E4 regions
JP4354814B2 (ja) 2001-09-29 2009-10-28 ヨンセイ ユニバーシティ 改善された疾患治療効果を有する組換えアデノウィルス及びそれを含む薬剤学的組成物
PL209133B1 (pl) 2001-11-21 2011-07-29 Univ Pennsylvania Rekombinowany adenowirus, obejmująca go izolowana komórka gospodarza, oraz kompozycja i zastosowanie
CN100475966C (zh) 2001-11-23 2009-04-08 上海三维生物技术有限公司 具有肿瘤细胞特异性感染和转基因表达能力的新型腺病毒
EP1327688A1 (en) 2002-01-14 2003-07-16 Vereniging Voor Christelijk Wetenschappelijk Onderwijs Adenoviruses with enhanced lytic potency
US20040002060A1 (en) 2002-01-24 2004-01-01 Novartis Ag Fiber shaft modifications for efficient targeting
JP2005515784A (ja) 2002-02-01 2005-06-02 トランジェーヌ、ソシエテ、アノニム Ｐｏｄに関連した細胞活性を調節するためのアデノウイルスベクター
US20030220284A1 (en) 2002-02-22 2003-11-27 Patricia Yotnda Delivery of adenoviral DNA in a liposomal formulation for treatment of disease
US20040005710A1 (en) 2002-03-09 2004-01-08 Ho-Sun Son Method for producing recombinant viruses using site-specific recombination
MXPA04009204A (es) 2002-03-26 2004-11-26 Oncolytics Biotech Inc Uso de adenovirus mutados en genes va para el tratamiento del cancer.
US20060147420A1 (en) 2004-03-10 2006-07-06 Juan Fueyo Oncolytic adenovirus armed with therapeutic genes
SI1497438T1 (sl) 2002-04-25 2010-03-31 Crucell Holland Bv Sredstva in postopki za pripravo adenovirusnih vektorjev
EP1499332A4 (en) 2002-04-29 2006-12-06 Hadasit Med Res Service COMPOSITIONS AND METHODS FOR TREATING CANCER WITH A VIRAL ONCOLYTIC AGENT
EP1504016A4 (en) 2002-05-08 2006-11-22 Intronn Inc USE OF SPLICEOSOM-MEDIATED RNA-TRANS-SPLICING TO GIVE ADENOVIRES A CELLSELECTIVE REPLICATION
US20060099178A1 (en) 2002-05-27 2006-05-11 Holm Per S Novel use of adenoviruses and nucleic acids coding therefor
AU2003247128A1 (en) 2002-07-10 2004-02-02 Transgene S.A. Modified adenoviral fiber with ablated to cellular receptors
US20040219516A1 (en) 2002-07-18 2004-11-04 Invitrogen Corporation Viral vectors containing recombination sites
US7364727B2 (en) 2002-07-22 2008-04-29 Cell Genesys, Inc. Metastatic colon cancer specific promoter and uses thereof
EP1539937A4 (en) 2002-08-22 2006-07-26 Merck & Co Inc METHODS OF PROPAGATION OF ADENOVIRUS AND VIRUS SO OBTAINED
JP2006500956A (ja) 2002-10-01 2006-01-12 デューク・ユニバーシティ 腫瘍の低酸素領域で選択的に複製する組み換えアデノウイルスベクターの使用による、標的腫瘍治療
EP1413586A1 (en) 2002-10-21 2004-04-28 Centre National De La Recherche Scientifique (Cnrs) Regulation of the Cre recombinase using a dissociation/re-association system of said recombinase
US20040102382A1 (en) 2002-11-27 2004-05-27 Transgene, S.A. Targeting peptides
US20080124360A1 (en) 2003-01-24 2008-05-29 Seggern Daniel J Von Adenovirus particles with enhanced infectivity of dendritic cells and particles with decreased infectivity of hepatocytes
JP2006513714A (ja) 2003-03-17 2006-04-27 メルク　エンド　カムパニー　インコーポレーテッド アデノウイルス血清型２４ベクター、核酸およびそれにより製造されたウイルス
US20040191761A1 (en) 2003-03-27 2004-09-30 Routes John M. Modified adenoviral E1A constructs and methods of use thereof
WO2004097016A1 (en) 2003-03-28 2004-11-11 Merck & Co., Inc. Adenovirus serotype 34 vectors, nucleic acids and virus produced thereby
WO2004087930A1 (en) 2003-03-28 2004-10-14 Saint Louis University Adenovirus replication-competent vectors expressing trail
US20050095705A1 (en) 2003-04-15 2005-05-05 Michael Kadan Method for production of oncolytic adenoviruses
US20060281090A1 (en) 2003-05-01 2006-12-14 University Of Washington Uw Tech Transfer- Invention Licensing Capsid-modified adenovirus vectors and methods of using the same
CA2524499A1 (en) 2003-05-14 2004-11-25 Istituto Di Ricerche Di Biologia Molecolare P. Angeletti Spa Broadening adenovirus tropism
US20050079158A1 (en) 2003-06-05 2005-04-14 Shenzhen Allucks Biotech Co., Ltd. Construct of anti-cancer recombinant adenovirus, method for preparing the same and use thereof
CA2527721C (en) 2003-06-10 2016-05-10 University Of Saskatchewan Chimeric adenovirus capsid proteins
US20070003923A1 (en) 2003-06-11 2007-01-04 Scripps Research Institute, The Modified fiber proteins for efficient receptor binding
US7291498B2 (en) 2003-06-20 2007-11-06 The Trustees Of The University Of Pennsylvania Methods of generating chimeric adenoviruses and uses for such chimeric adenoviruses
CA2528511C (en) 2003-06-20 2015-11-03 The Trustees Of The University Of Pennsylvania Methods of generating chimeric adenoviruses and uses for such chimeric adenoviruses
WO2005010149A2 (en) 2003-07-18 2005-02-03 Onyx Pharmaceuticals, Inc. Subgroup b adenoviral vectors for treating disease
WO2005012537A2 (en) 2003-07-25 2005-02-10 Genvec, Inc. Adenoviral vector-based vaccines
US20050186178A1 (en) 2003-08-28 2005-08-25 Ennist David L. Oncolytic adenoviral vectors encoding GM-CSF
WO2005030261A1 (en) 2003-08-28 2005-04-07 Cell Genesys, Inc. Oncolytic adenoviral vectors encoding gm-csf
EP1664099A2 (en) 2003-09-09 2006-06-07 Niels Rudi Pedersen Adenoviral epitopes
US7482156B2 (en) 2003-10-15 2009-01-27 Cell Genesys, Inc. Hepatocellular carcinoma specific promoter and uses thereof
WO2005051430A1 (de) 2003-11-14 2005-06-09 Per Sonne Holm Neue verwendung von adenoviren und dafür codierenden nukleinsäuren
US20050201978A1 (en) 2003-11-17 2005-09-15 Lipton James S. Tumor and infectious disease therapeutic compositions
US8354251B2 (en) 2003-12-31 2013-01-15 Kalobios Pharmaceuticals, Inc. Transactivation system for mammalian cells
WO2005075506A1 (en) 2004-01-09 2005-08-18 The Scripps Research Institute Identification of endogenous trimerization domains in the adenovirus fiber protein that allow detargeting and retargeting of viral vectors
CA2880060C (en) 2004-01-23 2018-03-13 Agostino Cirillo Chimpanzee adenovirus vaccine carriers
US20050201936A1 (en) 2004-02-12 2005-09-15 Wold William S.M. Models for viral-based cancer therapy
US7473418B2 (en) 2004-03-25 2009-01-06 Cell Genesys, Inc. Pan cancer oncolytic vectors and methods of use thereof
EP1729817B1 (en) 2004-03-30 2014-03-05 Industry-Academic Cooperation Foundation Gene delivery system containing relaxin gene and pharmaceutical composition using relaxin
US20080292592A1 (en) 2004-04-30 2008-11-27 Sunil Chada Oncolytic Adenovirus Armed with Therapeutic Genes
EP1743041B1 (en) 2004-05-03 2012-06-27 Stefan Kochanek Modified viral vector particles
GB0411428D0 (en) 2004-05-21 2004-06-23 Got A Gene Ab Vectors
JP4787936B2 (ja) 2004-05-26 2011-10-05 サイオクサス セラピューティクス リミティド 癌処理への使用のためのキメラアデノウィルス
US20050271622A1 (en) 2004-06-03 2005-12-08 Shenzhen Allucks Biotech Co., Ltd. Construct of tumor-selective recombinant adenovirus, method for preparing the same and use thereof
WO2005117993A2 (en) 2004-06-04 2005-12-15 Genvec, Inc. Method of using adenoviral vectors with improved safety and efficacy for the treatment of cancer
CN100361710C (zh) 2004-06-07 2008-01-16 成都康弘生物科技有限公司 肿瘤细胞专一表达免疫调节因子gm-csf的溶瘤性腺病毒重组体的构建及其应用
US20060292682A1 (en) 2004-07-22 2006-12-28 Hawkins Lynda K Addition of transgenes into adenoviral vectors
GB0416487D0 (en) 2004-07-23 2004-08-25 Isis Innovation Modified virus
US7776322B2 (en) 2004-08-16 2010-08-17 Stefan Kochanek Modified viral vector particles
CA2589602A1 (en) 2004-09-01 2006-04-13 Genvec, Inc. Adenoviral vectors able to transduce apcs, potential use in immune response generation
WO2007040550A2 (en) 2004-10-25 2007-04-12 Novartis Ag Torc polynucleotides and polypeptides, and methods of use
US7943373B2 (en) 2004-09-29 2011-05-17 Oncolys Biopharma, Inc. Telomelysin/GFP-expressing recombinant virus
MX2007004188A (es) 2004-10-13 2007-10-11 Crucell Holland Bv Vectores adenovirales mejorados y usos de los mismos.
US20070122817A1 (en) 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
IL166049A0 (en) 2004-12-30 2006-01-15 Gavish Galilee Bio Appl Ltd Method for obtaining modified proteins and viruseswith intact native binding
WO2006070023A2 (en) 2004-12-31 2006-07-06 Per Sonne Holm Method for reversing multiple resistance in animal cells
WO2006070024A2 (de) 2004-12-31 2006-07-06 Per Sonne Holm E1 -minus adenoviren und deren verwendung
WO2006086357A2 (en) 2005-02-10 2006-08-17 Merck & Co., Inc. Adenovirus serotype 36 vectors, nucleic acid and viruses produced thereby
EP1851343A2 (en) 2005-02-11 2007-11-07 Merck and Co., Inc. Adenovirus serotype 26 vectors, nucleic acid and viruses produced thereby
KR100747646B1 (ko) 2005-02-25 2007-08-08 연세대학교 산학협력단 데코린 유전자를 포함하는 유전자 전달 시스템 및 이를 포함하는 약제학적 항종양 조성물
WO2006119449A2 (en) 2005-05-04 2006-11-09 Vectorlogics, Inc. Modified adenovirus containing a stabilized antibody
WO2007050128A2 (en) 2005-05-31 2007-05-03 Vectorlogics, Inc. Shielded adenoviral vectors and methods of use
EP1893636A2 (en) 2005-06-17 2008-03-05 Istituto Di Ricerche Di Biologia Molecolare P. Angeletti S.P.A. Hepatitis c virus nucleic acid vaccine
CA2620495A1 (en) 2005-08-31 2007-03-08 Genvec, Inc. Adenoviral vector-based malaria vaccines
KR100723009B1 (ko) 2005-11-30 2007-05-29 한국원자력연구원 인간 ｐ３１ 유전자를 함유하는 악성 종양 치료용 약학적조성물
ES2304281B1 (es) 2006-02-01 2009-08-12 Dnatrix Inc. Adenovirus oncoliticos para el tratamiento del cancer.
WO2007094653A1 (en) 2006-02-13 2007-08-23 Vereniging Voor Christelijk Hoger Onderwijs, Wetenschappelijk Onderzoek En Patientenzorg Adenovirus particles having a chimeric adenovirus spike protein, use thereof and methods for producing such particles.
WO2007103825A2 (en) 2006-03-02 2007-09-13 The Board Of Regents Of The University Of Texas System Combination therapy with oncolytic adenovirus
US20070292954A1 (en) 2006-04-21 2007-12-20 The Brigham And Women's Hospital, Inc. Generation of recombinant DNA by sequence-and ligation-independent cloning
DK2012822T3 (da) 2006-04-28 2010-05-25 Univ Pennsylvania Modificeret adenovirushexonprotein og anvendelser deraf
US20080242608A1 (en) 2006-06-02 2008-10-02 Azad Bonni Methods and compositions for treating and preventing neurologic disorders
WO2007148148A1 (en) 2006-06-19 2007-12-27 Institut Gustave Roussy Inhibition of the liver tropism of adenoviral vectors
JP2008048621A (ja) 2006-08-22 2008-03-06 Chiba Prefecture キメラ型アデノウイルスとその作製方法並びにそれを用いた医薬
GR20060100496A (el) 2006-09-01 2008-04-15 Παρθενιος Μπουλικας ΛΙΠΟΣΩΜΑΤΙΚΑ ΕΓΚΑΨΥΛΙΩΜΕΝΟΙ ΦΟΡΕΙΣ-ΥΒΡΙΔΙΟΥ ΑΔΕΝΟΙΟΥ - ΙΟΥ SEMLIKI FOREST (SFV), ΠΟΥ ΦΕΡΕΙ ΚΑΤΑΣΚΕΥΑΣΜΑΤΑ RNAi ΚΑΙ ΘΕΡΑΠΕΥΤΙΚΑ ΓΟΝΙΔΙΑ ΠΟΥ ΧΡΗΣΙΜΟΠΟΙΟΥΝΤΑΙ ΕΝΑΝΤΙΟΝ ΣΤΟΧΩΝ ΚΑΡΚΙΝΟΥ ΚΑΙ ΑΛΛΩΝ ΑΣΘΕΝΕΙΩΝ
WO2008067025A2 (en) 2006-09-29 2008-06-05 Evanston Northwestern Healthcare Oncolytic adenoviruses and uses thereof
WO2008140474A1 (en) 2006-10-26 2008-11-20 Johns Hopkins University Recombinant adenovirus vaccines
US8207180B2 (en) 2006-11-28 2012-06-26 Nerviano Medical Sciences S.R.L. Tricyclic indoles and (4,5-dihydro) indoles
WO2008095168A2 (en) 2007-02-01 2008-08-07 University Of Chicago Compositions and methods related to a recombinant adenoviral vector that targets il 13 receptors
DK2137301T3 (da) 2007-03-14 2011-09-12 Inst Catala D Oncologia Adenovirus med mutationer i det endoplasmatiske reticulums retentionsdomæne for E3-19K-proteinet samt deres anvendelse ved cancerbehandling
WO2008150496A2 (en) 2007-05-31 2008-12-11 Genelux Corporation Assay for sensitivity to chemotherapeutic agents
WO2009065800A1 (en) 2007-11-20 2009-05-28 Crucell Holland B.V. Recombinant human adenoviruses for eliciting mucosal immune responses
US8231880B2 (en) 2007-11-28 2012-07-31 The Trustess Of The University Of Pennsylvania Simian subfamily C adenoviruses SAdV-40, -31, and -34 and uses thereof
SI2220242T1 (sl) 2007-11-28 2017-05-31 The Trustees Of The University Of Pennsylvania Podskupina opičjih b adenovirusov sadv-28,27,-29,-32,-33 in -35, in njihova uporaba
HUE032142T2 (en) 2007-11-28 2017-08-28 Univ Pennsylvania Simian E Adenovirus Adenovirus addovirus containing the SAdV-39 capsid hexon protein and its applications
EP2252316A2 (en) 2008-02-07 2010-11-24 Andrew Baker Modulation of adenoviral tropism
CN102016011B (zh) 2008-03-04 2013-12-11 宾夕法尼亚大学托管会 猿猴腺病毒sadv-36、-42.1、-42.2和-44及其应用
WO2009117656A2 (en) 2008-03-21 2009-09-24 Vectorlogics,Inc. Capsid-incorporated antigen for novel adenovirus vaccine
EP2300053A1 (en) 2008-06-04 2011-03-30 Cornell University Vaccines for prevention and treatment of addiction
JP5596026B2 (ja) 2008-06-19 2014-09-24 ミレニアム ファーマシューティカルズ， インコーポレイテッド チオフェン又はチアゾール誘導体及びｐｉ３ｋ阻害剤としてのそれらの使用
KR101034811B1 (ko) 2008-08-25 2011-05-16 단국대학교 산학협력단 조직 특이적 프로모터와 암 특이 유전자를 타겟팅하는트랜스-스플라이싱 라이보자임을 포함하는 재조합아데노바이러스 및 이의 용도
US8808986B2 (en) 2008-08-27 2014-08-19 Gen9, Inc. Methods and devices for high fidelity polynucleotide synthesis
KR20110081222A (ko) 2008-09-26 2011-07-13 어번 유니버시티 비복제성 벡터화된 백신의 점막 투여에 의한 조류의 면역화
JP5809978B2 (ja) 2008-10-31 2015-11-11 ザ・トラステイーズ・オブ・ザ・ユニバーシテイ・オブ・ペンシルベニア サルアデノウイルスＳＡｄＶ−４３、−４５、−４６、−４７、−４８、−４９および−５０ならびにそれらの用途
US9345787B2 (en) 2008-12-22 2016-05-24 Targovax Oy Adenoviral vectors and methods and uses related thereto
SG173432A1 (en) 2008-12-22 2011-09-29 Oncos Therapeutics Oy Oncolytic adenoviral vectors and methods and uses related thereto
CA3108979A1 (en) 2009-02-02 2010-08-05 Glaxosmithkline Biologicals Sa Simian adenovirus nucleic acid- and amino acid-sequences, vectors containing same, and uses thereof
PT3029144T (pt) 2009-03-02 2019-10-28 Univ California Mutantes e1a e e1b de adenovírus seletivos de tumor
ES2385251B1 (es) 2009-05-06 2013-05-06 Fundació Privada Institut D'investigació Biomèdica De Bellvitge Adenovirus oncolíticos para el tratamiento del cáncer.
US8846031B2 (en) 2009-05-29 2014-09-30 The Trustees Of The University Of Pennsylvania Simian adenovirus 41 and uses thereof
MX2012001404A (es) 2009-07-31 2012-06-01 Paxvax Inc Vectores de base adenoviral.
WO2011022002A1 (en) 2009-08-18 2011-02-24 The Rockefeller University Modification of recombinant adenovirus with immunogenic plasmodium circumsporozoite protein epitopes
US9555089B2 (en) 2009-08-18 2017-01-31 The Rockefeller University Modification of recombinant adenovirus with immunogenic plasmodium circumsporozoite protein epitopes
KR101248912B1 (ko) 2009-12-31 2013-03-29 한양대학교 산학협력단 항혈관신생 활성을 가지는 재조합 아데노바이러스
US9163261B2 (en) 2010-02-22 2015-10-20 Koteswara Rao KOLLIPARA Adeno-associated virus 2/8—micro RNA-101 therapy for liver cancer
CN102191245B (zh) 2010-03-15 2013-11-13 浙江东方基因生物制品有限公司 应用肿瘤特异性启动子表达报告基因来检测循环血中肿瘤细胞的方法和试剂
CN102892429B (zh) 2010-03-17 2016-08-31 康奈尔大学 基于被破坏的腺病毒的抗滥用药物疫苗
CN102844329B (zh) 2010-04-14 2016-01-20 财团法人牧岩生命工学研究所 分离自猿腺病毒血清型19的六邻体、其高变区和使用其的嵌合型腺病毒
WO2011133040A2 (en) 2010-04-23 2011-10-27 Orca Therapeutics B.V. Replication-competent adenoviruses
WO2011136400A1 (en) 2010-04-26 2011-11-03 Green Cross Corporation Tumor-specific promoter and oncolytic virus vector comprising the same
AU2011265260C1 (en) 2010-06-10 2016-03-17 University Of Washington Through Its Center For Commercialization Methods and systems for adenovirus interaction with desmoglein 2 (DSG2)
WO2012003287A2 (en) 2010-06-30 2012-01-05 The Johns Hopkins University Compositions and methods for detecting and quantifying circulating tumor cells ctcs
CN107090440B (zh) 2010-08-16 2021-10-22 萨克生物研究学院 腺病毒组装方法
BR112013003579A2 (pt) 2010-08-16 2016-06-07 Salk Inst For Biological Studi adenovírus, células, e, métodos para tratar câncer
WO2012022496A2 (en) 2010-08-19 2012-02-23 Per Sonne Holm Method for killing tumor stem cells
KR20130108371A (ko) 2010-09-24 2013-10-02 온코스 테라퓨틱스 오와이 단클론 항―ｃｔｌａ－４ 항체를 암호화하는 종양분해 아데노바이러스 벡터
BR112013006669A2 (pt) 2010-09-24 2019-09-24 Oncos Therapeutics Oy vetores adenovirais oncolíticos e métodos e usos relacionados aos mesmos
EP3075860A1 (en) 2010-11-23 2016-10-05 The Trustees of the University of Pennsylvania Subfamily e simian adenovirus a1295 and uses thereof
WO2012083297A2 (en) 2010-12-17 2012-06-21 Genvec, Inc. Adenoviral vectors with modified hexon regions
WO2012088041A1 (en) 2010-12-20 2012-06-28 Genvec, Inc. Adenoviral vector-based dengue fever vaccine
CN102174479B (zh) 2011-03-02 2013-04-03 北京锤特生物科技有限公司 靶向性治疗人肿瘤的溶肿瘤腺病毒及其应用
JP5975352B2 (ja) 2011-03-25 2016-08-23 国立大学法人 鹿児島大学 癌幹細胞を標的とするウイルスベクター
US20140341857A1 (en) 2011-05-16 2014-11-20 Institut Gustave Roussy Combination of oncolytic adenoviruses with histone deacetylase inhibitors
WO2012162342A2 (en) 2011-05-23 2012-11-29 The Wistar Institute Of Anatomy And Biology Influenza vaccines containing modified adenovirus vectors
GB201108879D0 (en) 2011-05-25 2011-07-06 Isis Innovation Vector
CN102260712B (zh) 2011-05-31 2013-10-02 北京锤特生物科技有限公司 溶肿瘤能力增强的B型人腺病毒Ad11突变体的构建和应用
RU2642293C2 (ru) 2011-08-23 2018-01-24 Нэшинел Институт оф Биомедикал Инновайшен Условно реплицирующий аденовирус
US20140348791A1 (en) 2011-09-09 2014-11-27 Beth Israel Deaconess Medical Center, Inc. Modified adenoviral vectors and methods of treatment using same
BR112014008249B1 (pt) 2011-10-05 2022-03-15 Genvec Inc Adenovírus ou vetor adenoviral e composição com os mesmos
EP2764119A2 (en) * 2011-10-05 2014-08-13 Genelux Corporation Method for detecting replication or colonization of a biological therapeutic
FI123955B (en) 2011-11-25 2014-01-15 Oncos Therapeutics Ltd Oncolytic adenovirus
EP2798069B1 (en) 2011-12-15 2017-03-29 Washington University Porcine knob xenotype chimeric adenoviral vector for dendritic cell infection
BR112014019049A2 (pt) 2012-02-02 2017-07-04 Univ Texas adenovirus imunogênico
CA2864956C (en) 2012-03-12 2021-11-09 Crucell Holland B.V. Batches of recombinant adenovirus with altered terminal ends
CN110592123A (zh) 2012-03-13 2019-12-20 萨克生物研究学院 使用腺病毒和化学二聚体的选择性细胞寻靶
JP6329936B2 (ja) 2012-03-14 2018-05-23 ソーク インスティテュート フォー バイオロジカル スタディーズ アデノウイルス腫瘍診断
US9017672B2 (en) 2012-05-11 2015-04-28 Immunicum Aktiebolag Hexon Tat-PTD modified adenovirus and uses thereof
WO2013173702A2 (en) 2012-05-18 2013-11-21 The Trustees Of The University Of Pennsylvania Subfamily e simian adenoviruses a1302, a1320, a1331 and a1337 and uses thereof
WO2013174910A1 (en) 2012-05-24 2013-11-28 Crucell Holland B.V. Adenoviral vectors for transduction of vascular tissue
WO2013181128A1 (en) 2012-05-29 2013-12-05 Genvec, Inc. Modified serotype 28 adenoviral vectors
EP2900818B1 (en) 2012-09-25 2017-06-07 University of Washington through its Center for Commercialization Desmoglein 2 (dsg2) binding proteins and uses therefor
KR101429696B1 (ko) 2012-11-21 2014-08-13 국립암센터 안전성 및 항암활성이 증가된 재조합 아데노바이러스 및 이의 용도
KR20150122674A (ko) 2013-02-28 2015-11-02 싸이오서스 테라퓨틱스 엘티디. 아데노바이러스의 생산 방법
WO2014160475A1 (en) 2013-03-13 2014-10-02 Aboody Karen S Tropic cell based virotherapy for the treatment of cancer
CA2903582C (en) 2013-03-14 2021-06-08 Salk Institute For Biological Studies Oncolytic adenovirus compositions
KR102502974B1 (ko) 2013-04-18 2023-02-23 틸트 바이오세러퓨틱스 오이 향상된 입양 세포 치료
SG10201710528WA (en) 2013-06-18 2018-01-30 Dnatrix Inc Treatment of brain cancer with oncolytic adenovirus
JP2016538327A (ja) 2013-09-24 2016-12-08 ユニバーシティ オブ ワシントン スルー イッツ センター フォー コマーシャリゼーション デスモグレイン２（ｄｓｇ２）結合タンパク質およびその使用
CA2931322A1 (en) 2013-11-22 2015-05-28 Dnatrix, Inc. Adenovirus expressing immune cell stimulatory receptor agonist(s)
GB201415579D0 (en) 2014-09-03 2014-10-15 Psioxus Therapeutics Ltd A process
EP2940128A1 (en) 2014-04-30 2015-11-04 Institut d'Investigació Biomèdica de Bellvitge (IDIBELL) Adenovirus comprising an albumin-binding moiety
JP6705755B2 (ja) 2014-05-19 2020-06-03 バロ セラピューティクス オイＶａｌｏ Ｔｈｅｒａｐｅｕｔｉｃｓ Ｏｙ 癌ワクチン開発のための改変アデノウイルス
EP3150706B1 (en) 2014-05-28 2019-07-10 National University Corporation Okayama University Conditionally replicating adenovirus to express reic gene
US10617729B2 (en) 2014-12-24 2020-04-14 The Uab Research Foundation Multitargeting onocolytic adenovirus, methods of use, and methods of making
WO2016118433A1 (en) 2015-01-20 2016-07-28 Shayakhmetov Dmitry M Detargeted adenovirus variants and related methods
US20180051301A1 (en) 2015-03-02 2018-02-22 Washington University Induction of pacemaker-like cells from cardiomyocytes
WO2016146894A1 (en) 2015-03-17 2016-09-22 Tilt Biotherapeutics Oy Oncolytic adenoviruses coding for bi-specific antibodies and methods and uses related thereto
EP3072900A1 (en) 2015-03-27 2016-09-28 Medizinische Hochschule Hannover Anti-tumour medicament based on adenovirus
GB201505860D0 (en) 2015-04-07 2015-05-20 Agalimmune Ltd Therapeutic compositions and methods of use for treating cancer
GB201513176D0 (en) 2015-07-27 2015-09-09 Glaxosmithkline Biolog Sa Novel methods for inducing an immune response
WO2017039313A1 (ko) 2015-09-01 2017-03-09 한양대학교 산학협력단 ＩＬ-12 및 ｓｈＶＥＧＦ 동시발현 아데노바이러스를 포함하는 항종양 면역성 증진 조성물
AU2016333996B2 (en) 2015-10-05 2020-05-28 Salk Institute For Biological Studies Synthetic adenoviruses with tropism to damaged tissue for use in promoting wound repair and tissue regeneration
EP3365062B1 (en) 2015-10-19 2024-09-18 CG Oncology, Inc. Methods of treating solid or lymphatic tumors by combination therapy
WO2017075395A1 (en) 2015-10-28 2017-05-04 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Tumor-specific adenovirus vectors and therapeutic uses
EP4155411A1 (en) 2016-02-23 2023-03-29 Salk Institute for Biological Studies Exogenous gene expression in therapeutic adenovirus for minimal impact on viral kinetics
JP7054527B2 (ja) 2016-02-23 2022-04-14 ソーク インスティテュート フォー バイオロジカル スタディーズ アデノウイルスの複製動態を測定するための高スループットアッセイ
EP3426271A4 (en) 2016-03-10 2019-10-16 Cold Genesys, Inc. METHODS OF TREATING SOLID OR LYMPHATIC TUMORS BY POLYTHERAPY
US20170314044A1 (en) 2016-05-02 2017-11-02 Regents Of The University Of Minnesota Adenovirus constructs and methods
US20190201551A1 (en) 2016-05-23 2019-07-04 Washington University Pulmonary targeted cas9/crispr for in vivo editing of disease genes
WO2017205875A1 (en) 2016-05-27 2017-11-30 Dnatrix, Inc. Adenovirus and immunomodulator combination therapy
GB2549809C (en) 2016-06-23 2022-11-30 Univ Oxford Innovation Ltd Vector
US11155832B2 (en) 2016-09-30 2021-10-26 Genvec, Inc. Adenovectors for delivery of therapeutic genetic material into T cells
FI20165814A (fi) 2016-10-27 2018-04-28 Tilt Biotherapeutics Oy Interleukiini 8 (il-8) prognostisena ja ennustavana biomarkkerina ja koostumukset ja vektorit käytettäväksi onkolyyttisessa immunoterapiassa
CN110072550A (zh) 2016-11-01 2019-07-30 德那翠丝有限公司 用于治疗脑癌的组合疗法
WO2018083257A1 (en) 2016-11-03 2018-05-11 Psioxus Therapeutics Limited Oncolytic adenovirus encoding transgenes
WO2018083259A1 (en) 2016-11-03 2018-05-11 Psioxus Therapeutics Limited Oncolytic adenovirus encoding transgenes
WO2018083258A1 (en) 2016-11-03 2018-05-11 Psioxus Therapeutics Limited Oncolytic adenovirus encoding at least three transgenes
CN109982709A (zh) 2016-11-17 2019-07-05 Vcn生物科学有限公司 病毒载体在治疗视网膜母细胞瘤中的用途
MX2019006722A (es) 2016-12-09 2019-08-22 Glaxosmithkline Biologicals Sa Construcciones de adenovirus de chimpance con antigenos de lyssavirus.
GB201620968D0 (en) 2016-12-09 2017-01-25 Glaxosmithkline Biologicals Sa Adenovirus polynucleotides and polypeptides
WO2018111767A1 (en) 2016-12-12 2018-06-21 Salk Institute For Biological Studies Tumor-targeting synthetic adenoviruses and uses thereof
US10232053B2 (en) 2016-12-30 2019-03-19 Trieza Therapeutics, Inc. Immunomodulatory oncolytic adenoviral vectors, and methods of production and use thereof for treatment of cancer
WO2018125970A1 (en) 2016-12-30 2018-07-05 Salk Institute For Biological Studies Synthetic adenoviruses targeting bone tissue and uses thereof
JP2020505049A (ja) 2017-01-30 2020-02-20 エピセントアールエックス，インコーポレイテッド 複数トランスジーン組み換えアデノウイルス
BR112019015514A2 (pt) 2017-01-30 2020-04-07 Epicentrx Inc mutantes de tata-box e caat-box seletivos de tumor
KR102643016B1 (ko) 2017-04-10 2024-02-29 에피센트알엑스, 인코포레이티드 재조합 바이러스의 제조 방법
EA201990822A1 (ru) 2017-04-12 2020-01-09 Эписентарикс, Инк. Иммуномодулирующие слитые белки
US11896634B2 (en) 2017-04-21 2024-02-13 Baylor College Of Medicine Oncolytic virotherapy with helper-dependent adenoviral-based vectors expressing immunomodulatory molecules
WO2018201017A1 (en) 2017-04-27 2018-11-01 Washington University Dendritic cell targeted adenovirus for vaccination
WO2018204677A1 (en) 2017-05-04 2018-11-08 Epicentrx, Inc. Oncolytic adenovirus formulation
JP7245175B2 (ja) 2017-05-24 2023-03-23 エピセントアールエックス，インコーポレイテッド 抗脈管形成アデノウイルス
CA3067909A1 (en) 2017-06-19 2018-12-27 Medicenna Therapeutics Inc. Uses and methods for il-2 superagonists, agonists, and fusions thereof
WO2019016756A1 (en) 2017-07-21 2019-01-24 Glaxosmithkline Biologicals Sa ANTIGEN CONSTRUCTS OF CHIKUNGUNYA VIRUSES
SI3460052T1 (sl) 2017-09-20 2020-03-31 Immunicum Ab Izboljšane alogene dendritične celice za uporabo pri zdravljenju raka
US12123027B2 (en) 2017-10-12 2024-10-22 Ascend Advanced Therapies Limited Life-cycle-defective adenovirus helper viruses, their production and use for producing rAAV
AU2018357912B2 (en) 2017-10-31 2023-11-09 Janssen Vaccines & Prevention B.V. Adenovirus and uses thereof
CA3077630A1 (en) 2017-10-31 2019-05-09 Janssen Vaccines & Prevention B.V. Adenovirus vectors and uses thereof
JP7438943B2 (ja) 2017-10-31 2024-02-27 ヤンセン ファッシンズ アンド プリベンション ベーフェー アデノウイルス及びその用途
EA202091074A1 (ru) 2017-10-31 2020-07-22 Янссен Вэксинс Энд Превеншн Б.В. Аденовирус и его применения
GB201802539D0 (en) 2018-02-16 2018-04-04 Univ College Cardiff Consultants Ltd Modified adenoviruses
GB201804473D0 (en) 2018-03-21 2018-05-02 Valo Therapeutics Oy Modified oncolytic adenoviruses
GB201804468D0 (en) 2018-03-21 2018-05-02 Valo Therapeutics Oy PeptiCRAd Cancer Therapy
US20210015878A1 (en) 2018-03-28 2021-01-21 Epicentrx, Inc. Personalized cancer vaccines
MX2020010499A (es) 2018-04-09 2020-10-28 Salk Inst For Biological Studi Composiciones de adenovirus oncoliticas con propiedades aumentadas de replicacion.
TW202011977A (zh) 2018-04-20 2020-04-01 貝勒醫學院 溶瘤病毒療法及免疫療法
CN112638936A (zh) 2018-06-12 2021-04-09 葛兰素史密丝克莱恩生物有限公司 腺病毒多核苷酸和多肽
WO2020014539A1 (en) 2018-07-11 2020-01-16 Epicentrx, Inc. Methods and compositions for targeting cancer cells for treatment
EP3844286A1 (en) 2018-08-31 2021-07-07 Orca Therapeutics B.V. Recombinant replication competent viruses comprising a coding region for glycogen synthase kinase-3 (gsk3) and methods of killing aberrant cells
EP3864159A4 (en) 2018-10-09 2022-11-02 Nikegen Limited COMPOSITIONS AND METHODS FOR THE PRODUCTION OF VIRAL VECTORS

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014000026A1 (en) * 2012-06-25 2014-01-03 University Of Canberra Recombinant viral vectors and uses therefor
WO2015155370A1 (en) * 2014-04-12 2015-10-15 Psioxus Therapeutics Limited Group b adenovirus modified in the e4orf4 region
WO2016049201A1 (en) * 2014-09-24 2016-03-31 Salk Institute For Biological Studies Oncolytic tumor viruses and methods of use

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GARTH M. FUNSTON等: "Expression of heterologous genes in oncolytic adenoviruses using picornaviral 2A sequences that trigger ribosome skipping", 《JOURNAL OF GENERAL VIROLOGY》 *
吴瑞倩 等: "基于内含肽的自剪切技术在膜蛋白研究中的应用", 《生命的化学》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109758467A (zh) * 2019-03-08 2019-05-17 中国农业科学院兰州兽医研究所 一种吉西他滨在制备预防口蹄疫病毒感染的药物中的应用

Also Published As

Publication number Publication date
CN108699566B (zh) 2023-06-30
ES2933174T3 (es) 2023-02-02
AU2017223589A1 (en) 2018-08-09
US10738325B2 (en) 2020-08-11
US20200325492A1 (en) 2020-10-15
EP4155411A1 (en) 2023-03-29
WO2017147269A1 (en) 2017-08-31
AU2023255054A1 (en) 2023-11-16
CN117384961A (zh) 2024-01-12
KR20180112024A (ko) 2018-10-11
JP7534798B2 (ja) 2024-08-15
JP2022051769A (ja) 2022-04-01
JP2019509037A (ja) 2019-04-04
EP3390645A1 (en) 2018-10-24
EP3390645B1 (en) 2022-09-14
AU2017223589B2 (en) 2023-08-03
JP7015551B2 (ja) 2022-02-15
KR102471633B1 (ko) 2022-11-25
US20180355379A1 (en) 2018-12-13
KR20220163505A (ko) 2022-12-09
US20230088476A1 (en) 2023-03-23
CA3013639A1 (en) 2017-08-31
US11401529B2 (en) 2022-08-02
KR20210065205A (ko) 2021-06-03

Similar Documents

Publication Publication Date Title
KR102471633B1 (ko) 바이러스 동역학에 미치는 영향 최소화를 위한 치료용 아데노바이러스의 외인성 유전자 발현
AU2022203504A1 (en) Oncolytic tumor viruses and methods of use
AU2020281047B2 (en) High throughput assay for measuring adenovirus replication kinetics
AU2011332025B2 (en) Subfamily E simian adenoviruses A1321, A1325, A1295, A1309 and A1322 and uses thereof
CN1833027B (zh) 产生嵌合腺病毒的方法及这种嵌合腺病毒的用途
KR101761425B1 (ko) 시미안 아데노바이러스 핵산- 및 아미노산-서열, 이를 포함하는 벡터 및 이의 용도
KR102535670B1 (ko) 아데노바이러스 폴리뉴클레오티드 및 폴리펩티드
KR101614364B1 (ko) 유인원 ｅ 아데노바이러스 ｓａｄｖ-39, -25.2, -26, -30, -37, 및 -38
DK2163260T3 (en) Chimpanzee adenovirus vaccine carriers
KR20200140848A (ko) 복제 속성이 향상된 종양살상형 아데노바이러스 조성물
CA2528511A1 (en) Methods of generating chimeric adenoviruses and uses for such chimeric adenoviruses
CN108135991A (zh) 新型腺病毒
CN107937440A (zh) 猴腺病毒(大猩猩)或腺病毒载体及其使用方法
CN107574154A (zh) 猴(大猩猩)腺病毒或腺病毒载体及其使用方法
RU2731356C9 (ru) Экспрессионный вектор для создания иммунобиологического средства для индукции специфического иммунитета против вируса тяжелого острого респираторного синдрома SARS-CoV-2 (варианты)
CN1993462A (zh) 腺病毒载体组合物
AU2016333996A1 (en) Synthetic adenoviruses with tropism to damaged tissue for use in promoting wound repair and tissue regeneration
KR20220027785A (ko) 신규한 코로나바이러스 재조합 스파이크 단백질, 이를 코딩하는 폴리뉴클레오티드, 상기 폴리뉴클레오티드를 포함하는 벡터 및 상기 벡터를 포함하는 코로나바이러스감염증 예방 또는 치료용 백신
CN112011570B (zh) 特异杀伤肿瘤细胞的溶瘤病毒系统及其应用
CN116323955A (zh) 通过crispr/cas介导的体内末端解析拯救重组腺病毒
CN114262692A (zh) 一种携带tmvp1和hsv-tk的溶瘤腺病毒重组体，其构建方法及应用
RU2821989C1 (ru) Новый аденовирусный вектор, не включающий компетентный по репликации аденовирус, и его применение
RU2744444C1 (ru) Применение средства для индукции специфического иммунитета против вируса тяжелого острого респираторного синдрома SARS-CoV-2 для ревакцинации населения (варианты)
KR20220106072A (ko) 복제가능 아데노바이러스를 포함하지 않는 신규 아데노바이러스 벡터 및 이의 용도
KR20230008069A (ko) 아데노바이러스 발현 벡터, 및 제조를 위한 방법 및 세포주

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant