KR102471633B1 - 바이러스 동역학에 미치는 영향 최소화를 위한 치료용 아데노바이러스의 외인성 유전자 발현 - Google Patents

바이러스 동역학에 미치는 영향 최소화를 위한 치료용 아데노바이러스의 외인성 유전자 발현 Download PDF

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KR102471633B1
KR102471633B1 KR1020217016040A KR20217016040A KR102471633B1 KR 102471633 B1 KR102471633 B1 KR 102471633B1 KR 1020217016040 A KR1020217016040 A KR 1020217016040A KR 20217016040 A KR20217016040 A KR 20217016040A KR 102471633 B1 KR102471633 B1 KR 102471633B1
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recombinant adenovirus
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클로다그 오'시어
윌리엄 패틀로
콜린 파워스
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솔크 인스티튜트 포 바이올로지칼 스터디즈
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Abstract

외인성 오픈 리딩 프레임(ORF) 및 자가-절단 펩타이드 암호화 서열을 포함하는 재조합 아데노바이러스 게놈이 기술된다. 바이러스 동역학에 최소한의 영향을 위한 외인성 유전자의 최적의 배치가 추가로 개시된다. 상기 재조합 아데노바이러스의 치료법의 적용 또한 기술된다.

Description

바이러스 동역학에 미치는 영향 최소화를 위한 치료용 아데노바이러스의 외인성 유전자 발현{EXOGENOUS GENE EXPRESSION IN THERAPEUTIC ADENOVIRUS FOR MINIMAL IMPACT ON VIRAL KINETICS}
관련 출원에 대한 교차 참조
본 출원은 2016년 2월 23일자로 출원된 미국 가출원 제62/298,653호의 이익을 주장하며, 이는 본원에 참조로서 포함된다.
기술분야
본 개시는 재조합 아데노바이러스 컨스트럭트(construct) 내 외인성(exogenous) 오픈 리딩 프레임(open reading frame)의 최적 배치 및 상기 재조합 바이러스의 치료법의 적용에 관한 것이다.
아데노바이러스 항원형 5(adenovirus serotype 5, Ad5)는 기본적인 연구 응용, 쥐(murine) 페암 모델, 및 인간 유전자 치료 시험에서 선택되는 벡터이다. 아데노바이러스는 특정 세포 유형의 수용체에 대해 감염을 표적으로 하는 Ad 파이버(fiber) 단백질 스파이크(spike)로 장식된 단백질 캡시드(capsid)에 의해 보호되는 안정한 36 kb 이중-가닥의 DNA 게놈을 가진다. 아데노바이러스는 숙주 DNA에 삽입되지 않고 확립된 프로토콜을 이용하여 높은 역가(titer)로 생산될 수 있으며, 인간 유전자 치료 및 암 적용에 입증된 안전성을 가진다. 따라서, Ad-기반의 벡터는 암 진단 및 치료법에 엄청난 유망성을 가지고 있다.
요약
본원에 이종의(heterologous) 오픈 리딩 프레임(open reading frame, ORF) 및 자가-절단(self-cleaving) 펩타이드 암호화 서열을 포함하는 재조합 아데노바이러스 게놈이 개시되어 있다. 상기 이종의 ORF는 예를 들어 치료용 단백질을 암호화할 수 있다. 상기 재조합 아데노바이러스 게놈 및 개시된 게놈에 의해 생산되는 재조합 바이러스는 예를 들어, 암 치료와 같은 치료법의 적용에 사용될 수 있다.
이종의 ORF 및 자가-절단 펩타이드 암호화 서열을 포함하고, 둘은 작동 가능하게 연결되고 내인성(endogenous) 아데노바이러스 ORF와 동일한 리딩 프레임 내에 있는 재조합 아데노바이러스 게놈이 본원에 제공된다. 상기 자가-절단 펩타이드 암호화 서열은 상기 이종의 ORF와 상기 내인성 ORF 사이에 위치한다. 일부 실시양태에서, 상기 내인성 ORF는 E1B-55k이고 상기 이종의 ORF는 E1B-55k의 3'이다; 상기 내인성 ORF는 DNA 중합효소(polymerase)이고 상기 이종의 ORF는 DNA 중합효소의 5'이다; 상기 내인성 ORF는 DNA-결합 단백질(DNA-binding protein (DBP))이고 상기 이종의 ORF는 DBP의 3'이다; 상기 내인성 ORF는 아데노바이러스 사멸 단백질(adenovirus death protein(ADP))이고 상기 이종의 ORF는 ADP의 5'이다; 상기 내인성 ORF는 E3-14.7k이고 상기 이종의 ORF는 E3-14.7k의 3'이다; 상기 내인성 ORF는 E4-ORF2이고 상기 이종의 ORF는 E4-ORF2이다; 또는 상기 내인성 ORF는 파이버(fiber)이고 상기 이종의 ORF는 파이버의 3'이다. 일부 실시예에서, 상기 이종의 ORF는 치료용 단백질을 암호화한다.
본원에 개시된 재조합 아데노바이러스 게놈을 포함하는 재조합 아데노바이러스가 본원에 추가로 제공된다. 재조합 아데노바이러스 게놈 또는 본원에 개시된 재조합 바이러스 및 약학적으로 허용가능한 염을 포함하는 조성물 또한 제공된다.
대상에 재조합 아데노바이러스 게놈 또는 본원에 개시된 재조합 아데노바이러스 (또는 이의 조성물)를 투여함으로써 대상에 치료용 단백질을 전달하는 방법 또한 제공된다. 이러한 방법에 있어서, 상기 재조합 아데노바이러스 게놈 또는 재조합 아데노바이러스의 이종의 ORF는 상기 치료용 단백질을 암호화한다.
재조합 아데노바이러스 게놈 또는 본원에 개시된 재조합 바이러스 (또는 이의 조성물)를 투여함으로써 종양 세포 생존율을 억제하고, 종양 세포 성장을 억제하고, 종양 진행을 억제하고, 종양 부피를 감소시키고, 암을 가진 대상을 치료하는 방법이 추가로 제공된다. 이러한 방법에 있어서, 상기 재조합 아데노바이러스 게놈 또는 재조합 아데노바이러스의 이종의 ORF는 암 치료에 적합한 치료용 단백질을 암호화한다.
본 개시의 상기 및 다른 목적과 특징은 첨부된 도면을 참조하여 진행되는 하기 상세한 설명으로부터 보다 명확해질 것이다.
도 1은 아데노바이러스 컨스트럭트를 시험하기 위한 예시적인 작업 흐름의 개략도이다. 전체 바이러스 게놈 플라스미드를 생산하고 다중(multi)-웰 플레이트에 있는 293-E4 세포와 같은 적절한 세포에 형질감염시킨다. 형질감염된 세포가 증식됨에 따라, 동결/해동시켜 바이러스 입자를 방출시킨 후 원심분리에 의해 세포 찌꺼기(debris)를 펠렛화한다. (바이러스 입자를 포함하는) 상층액을 다중의, 더 큰 배양 플레이트에 옮긴다. 형질감염된 세포로부터 바이러스 입자를 수확하고, CsCl는 정제하고 감염성 바이러스 역가는 ELISA에 의해 측정된다. 관심 세포 유형은 정제된 바이러스의 알려진 MOI로 감염된다. 감염 후 48 또는 72시간 째에, 아데노바이러스 후기(late) 단백질, 아데노바이러스 게놈 또는 플라크는 각각 웨스턴 블롯(Western blot), q-PCR 또는 플라크 어세이에 의해 측정된다.
도 2는 기하급수적(exponential) 바이러스 성장을 보여주는 개략도이다. 종양 내 모든 세포의 종양 살상(oncolytic killing)은 기하급수적 바이러스 성장을 필요로한다. 그러나, 대부분의 경우, 종양 세포의 작은 부분만 초기에 감염된다. 따라서, 복제 라운드(round) 당 자손수의 작은 차이는 단 몇 번의 복제 라운드 후에 입자 전체 수에 큰 차이를 초래한다. 사이클 당 3 비리온(virion)을 생산하는 바이러스와 사이클 당 5 비리온을 생산하는 바이러스 간의 비교를 나타낸다. 그래프에서 볼 수 있듯이, 5-6 라운드의 복제 후, 두 바이러스의 바이러스 역가가 유의하게 다르다.
도 3은 형광에 기초한 바이러스 동역학(fluorescence-based viral kinetic (FBVK)) 어세이의 예시적인 작업 흐름도를 보여주는 개략도이다. (예를 들어, Adsembly 또는 AdSLIC에 의해) 전체 바이러스 게놈 플라스미드를 생산하고 다중-웰 플레이트에 있는 관심 세포 유형을 형질감염시키는 데 사용한다. 대안적으로, 세포를 재조합 아데노바이러스 입자로 감염시킨다. 상기 아데노바이러스 게놈은 실질적으로 바이러스 복제 동영학을 변화시키지 않는 바이러스 게놈 내의 위치에서 형광 단백질을 암호화하는 하나 이상의 오픈 리딩 프레임(ORF)을 포함한다. 시간에 따른 형광을 모니터링하여 바이러스 복제 동역학을 계산한다. 종양살상(oncolytic) 바이러스 후보는 종양 세포와 정상 세포 사이의 바이러스 동역학에서 가장 큰 차이를 나타내는 것이다.
도 4a-4b는 아데노바이러스 게놈 플라스미드로 시작할 때 동역학 어세이 설정을 개략적으로 설명한다. 이 어세이는 초기 형질감염 효율에 대한 정확한 지식을 필요로 하지 않는다. 형질감염 조건을 초기에 형질감염된 세포의 약 5-10%가 되도록 선택한다. 나타낸 실시예에서, 48-웰 플레이트가 사용되며, 이는 3개의 거짓(mock)-감염된 웰과 FLUORESBRITETM 비드를 가진 3개의 웰과 함께, 14개의 상이한 바이러스 컨스트럭트를 3회 시험 가능하게 하여 도구 민감성 편차(drift)를 보완한다 (도 4a). 48-웰 플레이트의 상부 절반의 웰은 6개의 상이한 바이러스의 게놈 플라스미드, 거짓-감염된 세포 및 블랭크(FLUORESBRITETM 비드)로 형질감염된 세포를 각 3개씩 포함한다 (도 4b). 48-웰 플레이트의 하부 절반의 웰은 8개의 상이한 바이러스의 게놈 플라스미드로 형질감염된 세포를 포함한다. 연속적인 형광 모니터링을 위해 다중-웰 플레이트를 플레이트 리더(예를 들어 TECAN 플레이트 리더)에 놓는다.
도 5는 재조합 바이러스로 시작할 때 동역학 어세이 설정을 개략적으로 설명한다. 이 어세이는 바이러스 역가에 대한 정확한 지식을 필요로 하지 않는다. 재조합 바이러스를 연속적으로 희석하여 다중-웰 플레이트에 놓인 세포를 감염시티는 데 사용한다. 나타낸 실시예에서, 96-웰 플레이트가 사용되며 각 바이러스는 1:100, 1:300, 1:900, 1:2700, 1:8100, 1:24,300, 1:72,900 및 1:218,700로 희석하여 동시에 11개의 바이러스를 시험 가능하게 한다. 4개의 웰을 거짓-형질감염되고 FLUORESBRITETM 비드를 4개의 웰에 놓아 도구 민감성 및 편차를 보완한다. 연속적인 형광 모니터링을 위해 다중-웰 플레이트를 플레이트 리더(예를 들어 TECAN 플레이트 리더)에 놓는다.
도 6a-6c는 재조합 아데노바이러스의 조합 어셈블리에 대한 Adsembly 및 AdSLIC 기술의 개략적인 개요를 제공한다 (도 6a). 아데노바이러스 게놈은 4개의 모듈 - E1, 코어(core), E3 및 E4로 분리된다 (도 6b). Adsembly는 다중-부위 게이트웨이(Gateway) 반응을 사용하는 게놈 재-어셈블리를 포함한다 (도 6c). AdSLIC는 서열 및 라이게이션 독립적인 클로닝(sequence and ligation independent cloning (SLIC))을 이용하여 아데노바이러스 모듈을 어셈블한다.
도 7은 E1 영역의 형광 단백질을 암호화하는 재조합 아데노바이러스에 대한 ln-슬로프 값을 나타내는 막대 그래프이다. 각각 P2A 부위를 포함하는 직접적인 융합 컨스트럭트 YPet-E1A, 및 YPet-P2A-E1A, E1A-P2A-mCherry 및 E1B-55k-P2A-YPet 컨스트럭트에 대한 값을 나타낸다. YPet-P2A-ADP 컨스트럭트를 비교를 위해 나타낸다.
도 8은 형광에 기초한 바이러스 동역학 어세이에 대한 동역학 데이터 분석 및 해석의 개략도이다.
도 9a-9c는 Ad5, Ad9 또는 Ad34로부터 유래되고 이종의 ORF E3-14.7k ORF의 3' (또는 Ad9 및 Ad34에서 이의 등가물)를 포함하는 재조합 아데노바이러스에 대한 ln-슬로프 값을 나타내는 막대 그래프이다. 293 세포(도 9A), A549 세포(도 9B) 및 U2OS 세포(도 9C)의 Ad5 (E3-14.7k-P2A-YPet; PCMN-887), Ad9 (E3-15k-P2A-YPet; PCMN-888) 및 Ad34 (E3-14.8k-P2A-YPet; PCMN-889)에 대한 값을 나타낸다. 또한 각 도면에 (E3-14.7k-P2A-YPet을 포함하는) Ad5 코어 및 Ad9 (Ad5/Ad9) 또는 Ad34 (Ad5/Ad34)의 파이버 샤프트/손잡이(shaft/knob)를 포함하는 키메라 바이러스에 대한 값이 표시되어 있다.
서열목록
첨부되는 서열목록에 열거된 핵산 및 아미노산 서열은 C.F.R. 1.822에 정의된 바와 같이, 뉴클레오티드 염기에 대한 문자 약어 및 아미노산에 대한 3문자 코드를 이용하여 나타낸다. 각 핵산 서열의 단 하나의 가닥만 나타내지만, 상보적 가닥은 표시된 가닥에 대한 임의의 참고에 의해 포함되는 것으로 이해된다. 상기 서열 목록은 2017년 2월 21일에 생성된 730 KB의 ASCII 텍스트 파일로 제출되며, 이는 본원에 참고로 포함된다. 첨부된 서열 목록에서:
서열번호 1은 합성 아데노바이러스 게놈 CMBT-379 (YPet-P2A-E1A)의 뉴클레오티드 서열이다.
서열번호 2는 합성 아데노바이러스 게놈 CMBT-432 (E1A-P2A-YPet)의 뉴클레오티드 서열이다.
서열번호 3은 합성 아데노바이러스 게놈 CMBT-456 (E1B-55k-P2A-YPet)의 뉴클레오티드 서열이다.
서열번호 4는 합성 아데노바이러스 게놈 CMBT-499 (E1B-55k-P2A-mCherry)의 뉴클레오티드 서열이다.
서열번호 5는 합성 아데노바이러스 게놈 CMBT-530 (YPet-P2A-(DNA Poly))의 뉴클레오티드 서열이다.
서열번호 6은 합성 아데노바이러스 게놈 CMBT-886 (DBP-P2A-YPet)의 뉴클레오티드 서열이다.
서열번호 7은 합성 아데노바이러스 게놈 CMBT-403 (YPet-P2A-ADP)의 뉴클레오티드 서열이다.
서열번호 8은 합성 아데노바이러스 게놈 CMBT-429 (ADP-P2A-YPet)의 뉴클레오티드 서열이다.
서열번호 9는 합성 아데노바이러스 게놈 PCMN-887 (E3-14.7k-P2A-YPet)의 뉴클레오티드 서열이다.
서열번호 10은 합성 아데노바이러스 게놈 CMBT-457 (YPet-P2A-E4-ORF2)의 뉴클레오티드 서열이다.
서열번호 11은 합성 아데노바이러스 게놈 CMBT-633 (mCherry-P2A-E4-ORF2)의 뉴클레오티드 서열이다.
서열번호 12는 합성 아데노바이러스 게놈 CMBT-407 (YPet-P2A-Fiber)의 뉴클레오티드 서열이다.
서열번호 13은 합성 아데노바이러스 게놈 CMBT-445 (Fiber-P2A-YPet)의 뉴클레오티드 서열이다.
서열번호 14는 P2A의 아미노산 서열이다.
서열번호 15는 F2A의 아미노산 서열이다.
서열번호 16은 E2A의 아미노산 서열이다.
서열번호 17은 T2A의 아미노산 서열이다.
서열번호 18은 N-말단에 GSG를 포함하는 변형된 P2A의 아미노산 서열이다.
서열번호 19는 N-말단에 GSG를 포함하는 변형된 F2A의 아미노산 서열이다.
서열번호 20은 N-말단에 GSG를 포함하는 변형된 E2A의 아미노산 서열이다.
서열번호 21은 N-말단에 GSG를 포함하는 변형된 T2A의 아미노산 서열이다.
서열번호 22는 합성 아데노바이러스 게놈 PCMN-888 (Ad9 E3-15k-P2A-YPet)의 뉴클레오티드 서열이다.
서열번호 23은 합성 아데노바이러TM 게놈 PCMN-889 (Ad34 E3-14.8k-P2A-YPet)의 뉴클레오티드 서열이다.
상세한 설명
I. 약어
Ad 아데노바이러스
ADP 아데노바이러스 사멸 단백질(adenovirus death protein)
BFP 청색 형광 단백질(blue fluorescent protein)
E2A 마 비염 A 바이러스 2A(equine rhinitis A virus 2A)
ELISA 효소결합 면역흡착 어세이(enzyme-linked immunosorbent assay)
ERAV 마 비염 A 바이러스(equine rhinitis A virus)
F2A 수족구병 바이러스 2A(foot and mouth disease virus 2A)
FACS 형광 활성화 세포 분류(fluorescence activated cells sorting)
FMDV 수족구병 바이러스(food and mouth disease virus)
GFP 녹색 형광 단백질(green fluorescent protein)
MOI 감염 다중도(multiplicity of infection)
*OD 광학 밀도(optical density)
ORF 오픈 리딩 프레임(open reading frame)
P2A 돼지 테스코바이러스-1 2A(porcine teschovirus-1 2A)
pIX 단백질 IX(protein IX)
PTV1 돼지 테스코바이러스-1(porcine teschovirus-1)
RFP 적색 형광 단백질(red fluorescent protein)
SLIC 서열 및 라이게이션 독립적인 클로닝 (sequence and ligation independent cloning)
T2A 토세어 아시그나 바이러스 2A(Thosea asigna virus 2A)
TaV 토세어 아시그나 바이러스(Thosea asigna virus)
YFP 황색 형광 단백질(yellow fluorescent protein)
II. 용어 및 방법
달리 명시하지 않는 한, 기술적 용어는 일반적인 사용법에 따라 사용된다. 분자 생물학적에서 일반적인 용어의 정의는 Benjamin Lewin, Genes V, Oxford University Press에 의해 게재, 1994 (ISBN 0-19-854287-9); Kendrew 등 (eds.), The Encyclopedia of Molecular Biology, Blackwell Science Ltd.에 의해 게재 1994 (ISBN 0-632-02182-9); 및 Robert A. Meyers (ed.), Molecular Biology and Biotechnology: a Comprehensive Desk Reference, VCH Publishers, Inc.에 의해 게재, 1995 (ISBN 1-56081-569-8)에서 발견될 수 있다.
본 개시의 다양한 실시양태의 검토를 용이하게 하기 위해, 구체적인 용어의 하기 설명이 제공된다:
2A 펩타이드(2A peptide): 피코르나바이러스(picornaviruse)와 같은 일부 RNA 바이러스에 의해 암호화된 자가-절단 펩타이드의 유형. 2A 펩타이드는 리보솜이 2A 요소의 C-말단에서 펩타이드 결합의 형성을 거치지 않도록 함으로써 기능을 하며, 2A 서열의 말단과 하류 펩타이드 사이에서 분리를 유도한다 (Kim 등, PLoS One 6(4):e18556, 2011). "절단"은 상기 2A 펩타이드의 C-말단에서 발견되는 글리신 및 프롤린 잔기 사이에서 발생한다. 예시적인 2A 펩타이드는 본원에 서열번호 14-17로 기재되는 토세어 아시그나 바이러스(TaV), 마 비염 A 바이러스(ERAV), 돼지 테스코바이러스-1(PTV1) 및 수족구병 바이러스(FMDV)를 포함하나, 이에 한정되지 않는다. 일부 실시양태에서, 상기 2A 펩타이드는 절단 효율을 향상시키기 위해 N-말단에 있는 Gly-Ser-Gly를 포함한다 (서열번호 18-21).
아데노바이러스(Adenovirus): 선형, 이중-가닥의 DNA 게놈 및 정20면체(icosahedral) 캡시드를 갖는 비-피막 바이러스(non-enveloped virus). 현재 68개의 알려진 인간 아데노바이러스의 항원형이 있으며, 이는 7개의 종(A, B, C, D, E, F 및 G 종)으로 나누어진다. 상이한 아데노바이러스의 항원형은 상이한 질환의 종류, 호흡기 질환 (주로 B 및 C 종), 결막염(conjunctivitis) (B 및 D 종) 및/또는 위장염(gastroenteritis) (F 및 G 종)을 일으키는 일부 항원형과 관련된다.
아데노바이러스 사멸 단백질(Adenovirus death protein, ADP): 세포의 용해 및 다른 세포를 감염시키는 아데노바이러스의 방출을 매개하는 아데노바이러스 감염의 후기 단계에서 합성된 단백질. ADP는 핵막(nuclear membrane), 소포체(endoplasmic reticulum) 및 골지(Golgi)에 국한되는 101개 아미노산의 내재 막 당단백질(integral membrane glycoprotein)이다. ADP는 이전에 E3-11.6K로 명명했다.
투여(Administration): 임의의 효과적인 경로에 의해 개체에 치료용 제제(예, 재조합 바이러스)와 같은 제제를 제공 또는 주는 것. 예시적인 투여 경로는 주사(예를 들어, 피하(subcutaneous), 근육 내(intramuscular), 피내(intradermal), 복강 내(intraperitoneal), 종양 내(intratumoral) 및 정맥 내(intravenous)), 경구(oral), 관내(intraductal), 설하(sublingual), 직장(rectal), 경피(transdermal), 비강 내(intranasal), 질(vaginal) 및 흡입(inhalation) 경로를 포함하나, 이에 한정되지 않는다.
키메라(chimeric): 다른 기원을 적어도 두 부분으로 구성됨. 본 개시의 내용에서, "키메라 아데노바이러스"는 상이한 항원형 2개(예를 들어, Ad5 및 아데노바이러스의 제2 항원형으로부터) 이상으로부터 유래된 유전 물질 및/또는 단백질을 갖는 아데노바이러스이다. 이러한 맥락에서, "캡시드-교환된(capsid-swapped)" 아데노바이러스는 캡시드 단백질이 아데노바이러스의 하나의 항원형으로부터 유래되고 나머지 단백질이 다른 아데노바이러스 항원형으로부터 유래된 키메라 아데노바이러스를 의미한다. 유사하게, "키메라 파이버(fiber)"는 2개 이상의 다른 아데노바이러스의 항원형으로부터 유래된 아미노산 서열을 갖는 파이버 단백질이다. 예를 들어, 키메라 파이버는 Ad5의 파이버 샤프트(fiber shaft) 및 아데노바이러스 제2 항원형의 파이버 손잡이(fiber knob)으로 구성될 수 있다. 다른 실시예에서, 키메라 파이버는 Ad5 꼬리 및 아데노바이러스 제2 항원형(예를 들어, Ad9 또는 Ad34)의 파이버 샤프트 및 손잡이로 구성된다.
접촉(contacting): 직접적인 물리적 군집(association)에서의 배치(placement); 는 고체 및 액체 형태 모두를 포함한다.
퇴화된 변이(degenerate variant): 본 개시의 맥락에서, "퇴화된 변이"는 유전 코드의 결과로서 퇴화된 서열을 포함하는 펩타이드를 암호화하는 폴리뉴클레오티드를 말한다. 20개의 천연 아미노산이 있고, 대부분이 하나 이상의 코돈으로 지정된다. 따라서, 뉴클레오티드에 의해 암호화되는 펩타이드의 아미노산 서열이 변하지 않는 한, 펩타이드를 암호화하는 모든 퇴화된 뉴클레오티드 서열이 포함된다.
결실된(deleted): "결실된" 단백질(예를 들어, E4orf1 또는 E4orf6/7 단백질)을 암호화하는 아데노바이러스 게놈은 단백질 발현의 부재를 초래하는 단백질 암호화 서열의 완전한 결실, 또는 부분적 결실을 갖는 아데노바이러스를 의미한다.
E2F의 탈조절(deregulation): E2F 전사 인자 및 하류 표적 유전자의 활성 증가를 의미하며, 이는 인간 암의 거의 모든 종류에서 발생한다. E2F 경로 활성 및 전사의 탈조절은 예를 들어 Rb, p107 및 p130 종양 억제인자의 기능 상실 돌연변이 및 결실과 같이, 경로 중 임의의 상류 구성요소에서 다양한 상이한 돌연변이로 인해 발생할 수 있다. Rb는 확인된 최초의 종양 억제인자였고 인간 종양의 적어도 1/3에서 부재하거나 돌연변이되어 있다. 또한, p16 돌연변이 및/또는 후성적 침묵(epigenetic silencing)은 종양 세포에서 E2F를 활성화시킬 수 있다. 사이클린(cyclin) D 및 CDK4 돌연변이, 유전자 증폭 또는 과발현은 인간 종양에서 탈조절된 E2F 활성을 또한 초래할 수 있다. 또한 E2F는 EGFR, RTKs, RAS, RAF, PI-3K, PTEN, RAF, MYC을 포함하는 성장 인자 수용체(growth factor receptor) 경로 돌연변이에 의해 활성화된다. p16INK4a-사이클린 D:cdk4/6-RB-E2F 경로의 돌연변이는 일반적으로 상호 배타적(mutually exclusive) 방식으로 일어나므로, 하나의 '히트(hit)' (예를 들어, p16)는 다른 것들(예를 들어, Rb 돌연변이 또는 cyclin D:cdk 과발현)에 의해 동반되지 않는다. 그러나, 대부분 현재의 화학요법은 E2F 전사 표적을 억제하는 증식성 독이지만, 정상 세포에도 독성을 가지며 종종 치명적인 의원성(iatrogenic) 합병증을 가진다. 본원에 개시된 바와 같이, 대안적인 치료적 접근법은 p16-사이클린 D:cdk4-RB-E2F 경로를 탈조절하는 암세포 병변에서 선택적 용균 복제를 수행하는 바이러스를 이용하는 것이다.
DNA-결합 단백질(DNA-binding protein, DBP): 이 아데노바이러스 단백질은 이중-가닥의 DNA 뿐만 아니라 단일-가닥의 DNA 및 RNA에 결합한다. 72-kD 단백질인 DBP는 아데노바이러스 DNA의 복제에 필수적이다.
E1A: 아데노바이러스 초기 영역(E1A) 유전자 및 상기 유전자로부터 발현된 폴리펩타이드. 상기 E1A 단백질은 세포를 세포주기로 유도함으로써 바이러스 게놈 복제에 중요한 역할을 한다. 본원에 사용된 바와 같이, 용어 "E1A 단백질"은 E1A 유전자로부터 발현된 단백질을 의미하며, 상기 용어는 임의의 아데노바이러스 항원형에 의해 생산된 E1A 단백질을 포함한다.
E3-RIDα/RIDβ 및 E3-14.7k: E3 유전자로부터 생산된 초기-발현된 단백질. E3-RIDα, E3-RIDβ, 및 E3-14.7k 단백질은 수용체 내재화 및 분해(receptor internalization and degradation) 복합체를 구성하며, 핵막에 국한되고 숙주 항바이러스 반응으로부터 감염된 세포를 보호하기 위해 CD95 (FasL 수용체), 및 TNFR1 및 2 (TNF/TRAIL 수용체)를 포함하는 다양한 수용체의 내포 작용(endocytosis) 및 분해를 일으킨다. E3-RIDα, E3-RIDβ, 및 E3-14.7k 암호화 서열은 이 순서대로 서로 인접한다.
E4orf1: E4 유전자로부터 생산된 아데노바이러스 단백질. 용어 "E4orf1 단백질"은 임의의 아데노바이러스 항원형의 E4 유전자에 의해 생산된 E4orf1 단백질을 포함한다.
E4orf6/7: 아데노바이러스 E4 유전자에 의해 암호화되는 단백질. 용어 "E4orf6/7 단백질"은 임의의 아데노바이러스 항원형의 E4 유전자에 의해 생산된 E4orf6/7 단백질을 포함한다.
파이버(fiber): 아데노바이러스 파이버 단백질은 세포 표면 수용체에 결합을 매개하는 삼량체 단백질이다. 파이버 단백질은 긴 N-말단 샤프트 및 구형 C-말단 손잡이(knob)로 구성된다.
형광 단백질(Fluorescent protein): 특정 파장의 빛에 노출될 때 특정 파장의 빛을 방출하는 단백질. 형광 단백질은 녹색 형광 단백질(예, GFP, EGFP, AcGFP, Emerald, Superfolder GFP, Azami Green, mWasabi, TagGFP, TurboGFP 및 ZsGreen), 청색 형광 단백질(EBFP, EBFP2, Sapphire, T-Sapphire, Azurite 및 mTagBFP), 청록색 형광 단백질(cyan fluorescent protein) (예, ECFP, mECFP, Cerulean, CyPet, AmCyan1, Midori-Ishi Cyan, mTurquoise 및 mTFP1), 황색 형광 단백질(EYFP, Topaz, Venus, mCitrine, YPet, TagYFP, PhiYFP, ZsYellow1 및 mBanana), 주황색 형광 단백질(orange fluorescent protein) (Kusabira Orange, Kusabira Orange2, mOrange, mOrange2 및 mTangerine), 적색 형광 단백질 (mRuby, mApple, mStrawberry, AsRed2, mRFP1, JRed, mCherry, HcRed1, mRaspberry, dKeima-Tandem, HcRed-Tandem, mPlum, AQ143, tdTomato 및 E2-Crimson), 주황색/적색 형광 단백질 (dTomato, dTomato-Tandem, TagRFP, TagRFP-T, DsRed, DsRed2, DsRed-Express (T1) 및 DsRed-Monomer) 및 이의 변형된 버전을 포함하나, 이에 한정되지 않는다.
융합 단백질(Fusion protein): 2개 이상의 상이한(이종의) 단백질 또는 펩타이드의 아미노산 서열을 포함하는 단백질. 융합 단백질은 예를 들어, 2개의 상이한(이종의) 단백질의 부분을 암호화하는 핵산 서열로부터 조작된 핵산 서열의 발현에 의해 생성될 수 있다. 융합 단백질을 생성하기 위해, 상기 핵산 서열은 동일한 리딩 프레임 안에 있어야 하고 내부 정지 코돈을 포함하지 않아야 한다. 융합 단백질, 특히 짧은 융합 단백질은 또한 화학적 합성에 의해 생성될 수 있다.
이종(heterologous): 이종의 단백질 또는 폴리펩타이드는 상이한 공급원 또는 종으로부터 유래된 단백질 또는 폴리펩타이드를 말한다.
헥손(Hexon): 주요 아데노바이러스 캡시드 단백질.
면역조절제(immunomodulator): 면역계를 변화(예, 활성화, 향상 또는 억제)시키는 제제. 면역조절제는 사이토카인(cytokine) (예, 인터루킨 2(IL-2), IL-7, IL-12, GM-CSF, FLT3 리간드 또는 인터페론), 케모카인(chemokine) (예, CCL3, CCL26, CXCL7, CXCL9 및 CXCL10), T 세포 활성화 리간드(T cell activating ligand) (예, 항-CD3 Ab 또는 동종항원(alloantigen)), 동시-자극 분자(co-stimulatory molecule) (예, B7.1/B7.2, OX40L, 4-1-BBL 또는 CD40L), 면역관문 차단 억제제(checkpoint blockade inhibitor) (예, 항-PD-1 또는 항-CTLA4 Ab), 및 소분자 면역조절제(small molecule immunomodulator)를 포함하나, 이에 한정되지 않는다.
분리된(isolated): "분리된" 생물학적 구성요소(핵산 분자, 단백질, 바이러스 또는 세포)는 다른 염색체 및 과잉-염색체(extra-chromosomal)의 DNA 및 RNA, 단백질 및 세포와 같은 구성요소가 자연적으로 발생하는, 유기체의 세포 또는 조직에서 다른 생물학적 구성요소, 또는 유기체 그 자체로부터 실질적으로 분리 또는 정제되었다. "분리된" 핵산 분자 및 단백질은 표준 정제 방법에 의해 정제된 것들을 포함한다. 상기 용어는 또한 화학적으로 합성된 핵산 분자 및 단백질 뿐만 아니라 숙주 세포에서 재조합 발현에 의해 제조된 핵산 분자 및 단백질을 포괄한다.
변형(Modification): 핵산 또는 단백질 서열의 서열 변화. 예를 들어, 아미노산 서열 변형은 예를 들어, 치환, 삽입 및 결실, 또는 이의 조합을 포함한다. 삽입은 단일 또는 다중 아미노산 잔기의 서열내(intrasequence) 삽입 뿐만 아니라 아미노 및/또는 카르복실 말단 융합을 포함한다. 결실은 단백질 서열로부터 하나 이상의 아미노산 잔기를 제거하는 것을 특징으로 한다. 본원의 일부 실시양태에서, 상기 변형(예, 치환, 삽입 또는 결실)은 단백질 특정 활성의 감소 또는 증진과 같은 기능의 변화를 초래한다. 본원에 사용된 바와 같이, "Δ" 또는 "델타(delta)"는 결실을 의미한다. 치환 변형은 하나 이상의 잔기가 제거되고 그 자리에 다른 잔기가 삽입된 것이다. 아미노산 치환은 전형적으로 단일 잔기이나, 한 번에 많은 상이한 위치에서 발생할 수 있다. 치환, 결실, 삽입 또는 이의 임의의 조합을 조합하여 최종 돌연변이 서열을 얻을 수 있다. 이러한 변형은 단백질을 암호화하는 DNA에서 뉴클레오티드의 변형에 의해 제조될 수 있으며, 그렇게 함으로써 상기 변형을 암호화하는 DNA를 생산할 수 있다. 공지된 서열을 갖는 DNA 내 미리 결정된 부위에서 삽입, 결실 및 치환 돌연변이를 만드는 기술은 당업계에 잘 알려져 있다. "변형된" 단백질, 핵산 또는 바이러스는 상기 요약된 바와 같이 하나 이상의 변형을 갖는 것이다.
신생물(neoplasia), 악성 종양(malignancy), 암(cancer) 및 종양(tumor): 신생물(neoplasm)은 과도한 세포 분열로 인한 조직 또는 세포의 비정상적인 성장이다. 신생물의 성장은 종양을 생산할 수 있다. 개체(individual)의 종양의 양은 종양의 수, 부피, 또는 무게로 측정될 수 있는 "종양 부담(tumor burden)"이다. 전이하지 않은 종양을 "양성(benign)"이라 칭한다. 주변 조직을 침입 및/또는 전이할 수 있는 종양을 "악성(malignant)"이라 칭한다. 또한 악성 종양을 "암(cancer)"이라 칭한다.
혈액 암(hematologic cancer)은 혈액 또는 골수의 암이다. 혈액 (또는 혈행(hematogenous)) 암은 급성 백혈병(acute leukemia) (예, 급성 림프성 백혈병(acute lymphocytic leukemia), 급성 골수세포 백혈병(acute myelocytic leukemia), 급성 골수성 백혈병(acute myelogenous leukemia) 및 골수아구성(myeloblastic), 전골수구(promyelocytic), 골수단핵구(myelomonocytic), 단핵구(monocytic) 및 적백혈병(erythroleukemia)), 만성 백혈병(chronic leukemia) (예, 만성 골수세포(chronic myelocytic) (과립구) 백혈병, 만성 골수성 백혈병(chronic myelogenous leukemia), 및 만성 림프성 백혈병(chronic lymphocytic leukemia)), 진성 적혈구증가증(polycythemia vera), 림프종(lymphoma), 호지킨병(Hodgkin's disease), 비-호지킨 림프종(non-Hodgkin's lymphoma) (무통성 및 고도), 다발성 골수종(multiple myeloma), 발덴스트롬 마크로글로불린혈증(Waldenstrom's macroglobulinemia), 중쇄병(heavy chain disease), 골수이형성 증후군(myelodysplastic syndrome), 털세포 백혈병 및 골수이형성증(and myelodysplasia)을 포함하는 백혈병을 포함한다. 일부 경우에, 림프종(lymphoma)이 고형 종양으로 간주된다.
고형 종양은 낭종 또는 액체 영역을 일반적으로 포함하지 않는 조직의 비정상 덩어리이다. 고형 종양은 양성 또는 악성일 수 있다. 고형 종양의 다른 유형은 이들을 형성하는 세포의 유형으로 명명된다 (예, 육종(sarcomas), 암종(carcinomas), 및 림프종(lymphomas)). 육종 및 암종과 같은 고형 종양의 예는 섬유육종(fibrosarcoma), 점액육종(myxosarcoma), 지방육종(liposarcoma), 연골육종(chondrosarcoma), 골육종(osteosarcoma) 및 다른 육종, 활막종(synovioma), 중피종(mesothelioma), 유윙종양(Ewing's tumor), 평활근육종(leiomyosarcoma), 횡문근육종(rhabdomyosarcoma), 결장암(colon carcinoma), 림프성 종양(lymphoid malignancy), 췌장암(pancreatic cancer), 유방암(breast cancer), 폐암(lung cancer), 난소암(ovarian cancer), 전립선암(prostate cancer), 간세포 암종(hepatocellular carcinoma), 편평세포 암종(squamous cell carcinoma), 기저세포 암종(basal cell carcinoma), 선암종(adenocarcinoma), 한선 암종(sweat gland carcinoma), 갑상선 수질암(medullary thyroid carcinoma), 유두 갑상선 암종(papillary thyroid carcinoma), 갈색세포종 피지선 암종(pheochromocytomas sebaceous gland carcinoma), 유두 암종(papillary carcinoma), 인유두종 바이러스(human papilloma virus (HPV))-감염된 신생물, 유두 선암종(papillary adenocarcinomas), 수질암종(medullary carcinoma), 기관지 암종(bronchogenic carcinoma), 신장암(renal cell carcinoma), 간암(hepatoma), 담도암(bile duct carcinoma), 융모암(choriocarcinoma), 윌름스 종양(Wilms' tumor), 자궁경부암(cervical cancer), 고환 종양(testicular tumor), 정상피종(seminoma), 방광암(bladder carcinoma), 흑색종(melanoma), 및 CNS 종양 (예, 신경교종(glioma) (예 뇌간 신경교종(brainstem glioma) 및 혼합교종(mixed gliomas)), 교모세포종(glioblastoma) (또한 다형성 신경교종이라 알려짐) 별아 교세포종(astrocytoma), CNS 림프종(CNS lymphoma), 배아세포종(germinoma), 수모세포종(medulloblastoma), 신경초종(Schwannoma) 두개인두종(craniopharyogioma), 상의세포종(ependymoma), 송과체종(pinealoma), 혈관모세포종(hemangioblastoma), 청신경 종양(acoustic neuroma), 핍지교종(oligodendroglioma), 수막종(menangioma), 신경아세포종(neuroblastoma), 망막모세포종(retinoblastoma) 뇌 전이(brain metastasis))을 포함한다.
종양살상 바이러스(Oncolytic virus): 증식성 질환, 예를 들어, 암/종양 세포의 세포를 선택적으로 사멸시키는 바이러스. 암세포의 사멸은 살아있는 세포 수를 결정, 또는 세포병변 효과(cytopathic effect), 세포사멸(apoptosis), 또는 암세포 내 바이러스성 단백질의 합성 (예, 대사 표식, 면역블롯, 또는 복제에 필수적인 바이러스 유전자의 RT-PCR), 또는 종양의 크기를 검출하는 것과 같이 당업계에서 확립된 임의의 방법에 의해 검출될 수 있다.
작동가능하게 연결된(Operably linked): 제1 핵산 서열이 제2 핵산 서열과 기능적 관계에 놓일 때 제1 핵산 서열은 제2 핵산 서열과 작동 가능하게 연결된다. 예를 들어, 프로모터가 암호화 서열의 전사 또는 발현에 영향을 준다면 프로모터는 암호화 서열에 작동 가능하게 연결된다. 일반적으로, 작동 가능하게 연결된 DNA 서열은 연속적이며, 필요한 경우 두 개의 단백질-암호화 영역을 동일한 리딩 프레임에서 결합시킨다.
약학적으로 허용 가능한 담체(Pharmaceutically acceptable carrier): 본 개시에서 유용한 약학적으로 허용 가능한 담체(비히클)는 통상적이다. E. W. Martin에 의한 Remington’s Pharmaceutical Sciences, Mack Publishing Co., Easton, PA, 15th Edition (1975)는 하나 이상의 치료용 화합물, 분자 또는 제제(예를 들어, 본원에 개시된 재조합 바이러스)의 약학적 전달에 적합한 조성물 및 제제를 기술한다.
일반적으로, 담체의 성질은 사용되는 특정 투여 방식에 의존할 것이다. 예를 들어, 비경구용 제제는 보통 비히클로서 물, 생리 식염수, 평형 염액, 수성 덱스트로스, 글리세롤 등과 같이 약학적으로 및 생리학적으로 허용 가능한 유체를 포함하는 주사 가능한 유체를 포함한다. 고체 조성물 (예를 들어, 분말, 환제(pill), 정제(tablet), 또는 캡슐(capsule) 형태)의 경우, 통상적인 비독성 고체 담체는 예를 들어, 만니톨, 락토오스, 전분, 또는 마그네슘 스테아레이트의 약학적 등급을 포함할 수 있다. 생물학적으로-중성인 담체 이외에, 투여될 약학적 조성물은 습윤 또는 유화제, 방부제, 및 pH 완충제 등과 같은 소량의 비-독성 보조 물질, 예를 들어 소듐 아세테이트(sodium acetate) 또는 소르비탄 모노라우레이트(sorbitan monolaurate)를 포함할 수 있다.
폴리펩타이드, 펩타이드 또는 단백질(Polypeptide, peptide or protein): 단량체가 아미드 결합을 통해 함께 결합된 아미노산 잔기인 중합체. 아미노산이 α-아미노산인 경우, L-광학 이성질체 또는 D-광학 이성질체가 사용될 수 있다. 용어 "폴리펩타이드", "펩타이드" 및 "단백질"은 본 명세서에서 상호 교환적으로 사용된다. 이 용어는 자연적으로 발생하는 아미노산 중합체 및 비-자연적으로 발생하는 아미노산 중합체 뿐만 아니라 하나 이상의 아미노산 잔기가 상응하는 자연적으로 발생하는 아미노산의 인공적인 화학 모방체인 아미노산 중합체에 적용된다. 용어 "잔기" 또는 "아미노산 잔기"는 단백질, 폴리펩타이드 또는 펩타이드에 혼입된(incorporated) 아미노산에 대한 참조를 포함한다.
폴리펩타이드에서의 보존적 치환은 유사한 생화학적 특성을 갖는 상이한 아미노산 잔기에 대한 단백질 서열 내에서의 하나의 아미노산 잔기의 치환이다. 전형적으로, 보존적 치환은 생성된 폴리펩타이드의 활성에 거의 또는 전혀 영향을 미치지 않는다. 예를 들어, 하나 이상의 보존적 치환 (예를 들어, 1, 2, 3, 4 또는 5 이하의 치환)을 포함하는 단백질 또는 펩타이드는 야생형 단백질 또는 펩타이드의 구조 및 기능을 보유한다. 폴리펩타이드는 부위-특이적 돌연변이 유발(site-directed mutagenesis) 또는 PCR과 같이 표준 절차를 이용하여 폴리펩타이드를 암호화하는 뉴클레오티드 서열을 조작함으로써 하나 이상의 보존적 치환을 포함하도록 생성될 수 있다. 일 실시예에서, 이러한 변이체는 항체 교차-반응성(cross-reactivity) 또는 면역 반응을 유도하는 능력을 시험함으로써 용이하게 선택될 수 있다. 보존적 치환의 예는 하기와 같다.
Figure 112021060760163-pat00001
보존적 치환은 일반적으로 (a) 치환 영역 내 폴리펩타이드 백본의 구조, 예를 들어, 시트(sheet) 또는 나선(helical) 형태(conformation), (b) 표적 부위에서 분자의 전하 또는 소수성, 또는 (c) 측쇄의 부피(bulk)를 유지한다.
일반적으로 단백질 성질에서 가장 큰 변화를 가져올 것으로 예상되는 치환은 비-보존적일 것이며, 예를 들어, (a) 친수성 잔기, 예를 들어, 세릴(seryl) 또는 트레오닐(threonyl)은 소수성 잔기, 예를 들어, 류실(leucyl), 이소류실(isolecuyl), 페닐알라닐(phenylalanyl), 발릴(valyl) 또는 알라닐(alanyl)로 (또는 에 의해) 치환; (b) 시스테인 또는 프롤린은 다른 잔기로 (또는 에 의해) 치환; (c) 전기 양성(electroposition) 측쇄를 갖는 잔기, 예를 들어, 리실(lysyl), 아르기닐(arginyl) 또는 히스타딜(histadyl)은 전기 음성(electronegative) 잔기, 예를 들어 글루타밀(glutamyl) 또는 아스파틸(aspartyl)로 (또는 에 의해) 치환; 또는 (d) 부피가 큰 측쇄를 갖는 잔기, 예를 들어 페닐알라닌은 측쇄를 갖지 않는 잔기, 예를 들어 글리신으로 (또는 에 의해) 치환인 변화이다.
질환의 예방, 치료 또는 완화(Preventing, treating or ameliorating a disease): 질환을 "예방"하는 것은 질환의 완전한 발달을 억제하는 것을 의미한다. "치료"는 질환이나 병리학적 상태의 징후 또는 증상이 발달한 후, 질환이나 병리학적 상태의 징후 또는 증상을 완화시키는 치료적 개입을 의미한다. "완화"는 질환의 징후 또는 증상의 수 또는 중증도의 감소를 의미한다.
프로모터(Promoter): 핵산 (예, 유전자)의 전사를 지시/개시하는 DNA 영역. 프로모터는 전사의 개시 부위 부근에 필요한 핵산 서열을 포함한다. 전형적으로, 프로모터는 그들이 전사하는 유전자 근처에 위치한다. 프로모터는 선택적으로 원거리(distal) 인핸서 또는 전사의 개시 부위로부터 수천 염기쌍만큼 위치할 수 있는 억제자(repressor) 요소를 또한 포함한다. "구성적 프로모터(constitutive promoter)"는 연속적으로 활성이고 외부 신호 또는 분자에 의해 조절을 받지 않는 프로모터이다. 대조적으로, "유도성 프로모터(inducible promoter)"의 활성은 외부 신호 또는 분자 (예, 전사 인자 또는 테트라사이클린(tetracycline))에 의해 조절된다.
단백질 IX (Protein IX, pIX): 헥손 단백질과 연관된 아데노바이러스 캡시드의 부수적인 구성요소
정제된(Purified): 용어 "정제된"은 절대적인 순도를 요구하지 않는다; 오히려 그것은 상대적 용어로 의도된다. 따라서, 예를 들어, 정제된 펩타이드, 단백질, 바이러스 또는 다른 활성 화합물은 자연적으로 연관된 단백질 및 다른 오염물로부터 전체 또는 부분적으로 분리된 것이다. 특정 실시양태에서, 용어 "실질적으로 정제된"은 세포, 세포 배양 배지, 또는 다른 조제물(crude preparation)으로부터 분리된 펩타이드, 단백질, 바이러스 또는 다른 활성 화합물을 의미하고 초기 제제의 다양한 구성요소, 예를 들어 단백질, 세포 찌꺼기 및 다른 구성요소를 제거하기 위해 분획화되었다.
재조합(Recombinant): 재조합 핵산 분자, 단백질 또는 바이러스는 자연적으로 발생하지 않는 서열을 갖거나 그렇지 않으면 분리된 2개 서열의 인위적인 조합에 의해 만들어진 서열을 갖는 것이다. 이 인위적 조합은 화학적 합성 또는 유전적 조작 기술에 의한 것과 같이 핵산 분자의 분리된 부분의 인공적인 조작에 의해 달성될 수 있다. 용어 "재조합체"는 또한 천연 핵산 분자, 단백질 또는 바이러스의 일부의 첨가, 치환 또는 결실에 의해서만 변경된 핵산, 단백질 및 바이러스를 포함한다.
복제 결함(Replication defects): (종양 세포와 비교하여) 비-종양 세포에서 "복제 결함"을 나타내는 아데노바이러스는 종양 세포와 비교하여 정상 세포에서 감소된 바이러스 복제를 나타내는 아데노바이러스를 의미한다. 복제 결함은 예를 들어, 바이러스 후기 단백질 발현의 결핍, 바이러스 DNA 합성의 감소, E2F 표적 유전자(예, 사이클린 A 및 B)를 유도하는 능력 감소, S 단계 진입을 유도하는 능력 감소 및/또는 종양 세포와 비교하여 정상 세포에서 세포 사멸을 유도하는 능력 감소에 의해 입증된다.
복제 결함 바이러스(Replication deficient virus): 주어진 표현형을 갖는 미리 결정된 세포 집단 (예, 탈조절된 E2F 경로를 갖는 종양 세포)에서 우선적으로 세포 증식을 억제하거나, 세포 용해를 일으키거나 세포사멸 (집합적으로 세포사멸로 간주됨)을 유도하는 바이러스. 이러한 바이러스는 세포 증식을 억제하거나, 세포 용해를 일으키거나 세포사멸을 유도하는 것을 할 수 없거나 제한되어 있거나 그렇지 않으면 미리 결정된 세포 표현형 (예, 정상, 비-정상 세포)을 갖지 않는 세포에서 복제한다.
자가-절단 펩타이드(Self-cleaving peptide): 리보솜이 C-말단에서 펩타이드 결합의 합성을 건너 뛰도록 유도하는 펩타이드는 펩타이드 서열 및 하류 폴리펩타이드의 분리를 유도한다. 바이러스에 의해 암호화된 2A 펩타이드는 자가-절단 펩타이드의 일종이다. 바이러스에 의해 암호화된 2A 펩타이드는 예를 들어, 돼지 테스코바이러스-1 2A(PTV1), 수족구병 바이러스(FMDV), 마 비염 A 바이러스(ERAV) 및 토세어 아시그나 바이러스(TaV)를 포함한다.
서열 상동성(Sequence identity): 2개 이상의 핵산 서열 또는 2개 이상의 아미노산 서열 사이의 동일성 또는 유사성은 서열 간의 동일성 또는 유사성의 관점에서 표현된다. 서열 상동성은 퍼센트 동일성의 관점에서 측정될 수 있다; 퍼센트가 높을수록, 동일한 서열이 더 많다. 서열 유사성은 (보존적 아미노산 치환을 고려한) 퍼센트 유사성의 관점에서 측정될 수 있다; 퍼센트가 높을수록, 유사한 서열이 더 많다. 핵산 또는 아미노산 서열의 상동체(homolog) 또는 오솔로그(ortholog)는 표준 방법을 사용하여 정렬될 때 비교적 높은 정도의 서열 상동성/유사성을 갖는다. 이 상동성은 오솔로그 단백질(orthologous protein) 또는 cDNA가 (인간 및 C. elegans 서열과 같이) 더 먼 관련이 있는 종과 비교하여, (인간 및 마우스 서열과 같이) 더 밀접하게 관련이 있는 종으로부터 유도될 때 더 중요하다.
비교를 위한 서열의 정렬 방법은 당업계에 잘 알려져 있다. 다양한 프로그램 및 정렬 알고리즘은 Smith & Waterman, Adv. Appl. Math. 2:482, 1981; Needleman & Wunsch, J. Mol. Biol. 48:443, 1970; Pearson & Lipman, Proc. Natl. Acad. Sci. USA 85:2444, 1988; Higgins & Sharp, Gene, 73:237-44, 1988; Higgins & Sharp, CABIOS 5:151-3, 1989; Corpet et al., Nuc. Acids Res. 16:10881-90, 1988; Huang et al. Computer Appls. in the Biosciences 8, 155-65, 1992; 및 Pearson et al., Meth. Mol. Bio. 24:307-31, 1994. Altschul et al., J. Mol. Biol. 215:403-10, 1990에 설명되어 있고, 서열 정렬 방법 및 상동성 계산에 대한 자세한 고려사항을 제시한다.
BLAST(NCBI Basic Local Alignment Search Tool) (Altschul et al., J. Mol. Biol. 215:403-10, 1990)는 서열 분석 프로그램 blastp, blastn, blastx, tblastn 및 tblastx와 연결하기 위한 용도로 국립 생물 정보 센터 (NCBI, National Center for Biological Information)와 인터넷을 포함한 몇몇 출처로부터 이용할 수 있다. 추가 정보는 NCBI 웹 사이트에서 찾을 수 있다.
항원형(Serotype): 항원의 특징적인 세트에 의해 구별되는 밀접하게 관련된 미생물 그룹 (예, 바이러스).
대상(Subject): 인간과 비-인간 포유 동물을 포함하는 범주인 살아있는 다세포 척추 동물.
합성(Synthetic): 실험실에서 인위적인 방법으로 생산됨, 예를 들어 합성 핵산 또는 단백질은 실험실에서 화학적으로 합성될 수 있다.
치료제(Therapeutic agent): 대상에게 적절하게 투여될 때 원하는 치료 또는 예방 효과를 유도할 수 있는 임의의 제제. 치료제는 화학적 화합물, 소분자, 재조합 바이러스, 안티센스 화합물, 항체 (또는 이의 항원-결합 단편), 펩타이드 또는 핵산 분자를 포함 하나 이에 한정되지 않는다. 예를 들어, 암을 치료하기 위한 치료제는 종양 성장, 종양 발생, 또는 종양 전이를 예방 또는 억제하는 제제를 포함한다. "치료용 단백질"은 항체 또는 이의 항원-결합 단편을 포함하는 단백질 또는 펩타이드인 치료제이다. 본원의 일부 실시양태에서, 상기 치료용 단백질은 면역조절제이다. 다른 실시양태에서, 상기 치료용 단백질은 독소, Fas 또는 FasL, 가용성 사멸 인자, 방관자 파괴(bystander destruction)의 매개체, 종양 항원, 신 항원(neo antigen) 또는 동종항원을 포함한다.
치료학적으로 유효한 양(Therapeutically effective amount): 제제로 치료되는 대상 또는 세포에서 원하는 효과를 달성하기에 충분한 특정 약학적 또는 치료제 (예를 들어, 재조합 바이러스)의 양. 제제의 유효한 양은 치료 받는 대상 또는 세포 및 치료용 조성물의 투여 방식을 포함하나 이에 한정되지 않는 여러 인자에 좌우될 수 있다.
유엑손(Uexon): 초기 E3 영역과 파이버 유전자 (Tollefson et al., J Virol 81(23):12918-12926) 사이의 l 가닥 (좌측 전사)에 위치한 오픈 리딩 프레임.
달리 설명되지 않는 한, 본원에서 사용되는 모든 기술적 및 과학적 용어는 본 개시 내용이 속하는 기술 분야의 당업자에 의해 일반적으로 이해되는 것과 동일한 의미를 갖는다. 단수의 용어 "하나(a, an 및 the)"는 문맥에 달리 명시되어 있지 않는 한 복수 대상을 포함한다. "A 또는 B를 포함"은 A, 또는 B, 또는 A 및 B를 포함하는 것을 의미한다. 핵산 또는 폴리펩타이드에 대해 주어진 모든 염기 크기 또는 아미노산 크기, 및 모든 분자량(molecular weight) 또는 분자량(molecular mass) 값은 근사치이며 설명을 위해 제공되는 것으로 이해해야한다. 본원에 기술된 것들과 유사하거나 동등한 방법 및 재료가 본 개시의 실시 또는 테스트에 사용될 수 있지만, 적합한 방법 및 재료는 하기에 기술된다. 본 명세서에 언급된 모든 간행물, 특허 출원, 특허 및 기타 참고 문헌은 그 전체가 참고 문헌으로 인용된다. 충돌이 있는 경우, 용어의 설명을 포함하는 본 명세서가 우선한다. 또한, 재료, 방법 및 실시예는 단지 예시적인 것이며 제한하려는 것은 아님이 산 또는 폴리펩티드에 대해 주어진 것은 대략적인 것이며 설명을 위해 제공된다. 또한, 재료, 방법 및 실시예가 단지 예시적인 것이며 제한하려는 것은 아니다.
III. 실시양태의 개요
이종의 오픈 리딩 프레임(open reading frame (ORF)) 및 자가-절단 펩타이드 암호화 서열을 포함하는 재조합 아데노바이러스 게놈이 본원에 개시되어 있다. 상기 이종의 ORF는 예를 들어, 치료용 단백질 (예: 면역조절제)을 암호화할 수 있다. 상기 개시된 게놈에 의해 생성되는 재조합 아데노바이러스 게놈 및 재조합 아데노바이러스는 예를 들어, 암의 치료와 같은 다양한 상이한 치료법의 적용에 사용될 수 있다.
이종의(heterologous) 오픈 리딩 프레임(open reading frame, ORF) 및 자가-절단(self-cleaving) 펩타이드 암호화 서열을 포함하고, 둘은 작동 가능하게 연결되고 내인성(endogenous) 아데노바이러스 ORF와 동일한 리딩 프레임 내에 있는 재조합 아데노바이러스 게놈이 본원에서 제공된다. 상기 자기-절단 펩타이드 암호화 서열은 상기 이종의 ORF와 상기 내인성 ORF 사이에 위치한다. 일부 실시양태에서, 상기 내인성 ORF는 E1B-55k이고 상기 이종의 ORF는 E1B-55k의 3'이다; 상기 내인성 ORF는 DNA 중합효소(polymerase)이고 상기 이종의 ORF는 DNA 중합효소의 5'이다; 상기 내인성 ORF는 DNA-결합 단백질(DNA-binding protein (DBP))이고 상기 이종의 ORF는 DBP의 3'이다; 상기 내인성 ORF는 아데노바이러스 사멸 단백질(adenovirus death protein(ADP))이고 상기 이종의 ORF는 ADP의 5'이다; 상기 내인성 ORF는 E3-14.7k이고 상기 이종의 ORF는 E3-14.7k의 3'이다; 상기 내인성 ORF는 E4-ORF2이고 상기 이종의 ORF는 E4-ORF2이다; 또는 상기 내인성 ORF는 파이버(fiber)이고 상기 이종의 ORF는 파이버의 3'이다.
일부 실시양태에서, 상기 이종의 ORF는 치료용 단백질을 암호화한다.
일부 실시양태에서, 상기 자가-절단 펩타이드는 2A 펩타이드 또는 이의 변이체이다. 일부 실시예에서, 상기 2A 펩타이드는 돼지 테스코바이러스-1(PTV1) 2A (P2A) 펩타이드, 수족구병(FMDV) 2A (F2A) 펩타이드, 마 비염 A 바이러스(ERAV) 2A (E2A) 펩타이드 또는 토세어 아시그나 바이러스(TaV) 2A (T2A) 펩타이드 또는 이의 변이체이다. 특정 실시예에서, 상기 P2A 펩타이드 서열은 서열번호 14 또는 서열번호 18의 아미노산 서열과 적어도 80%, 또는 적어도 85%, 적어도 90%, 적어도 95%, 적어도 96%, 적어도 97%, 적어도 98% 또는 적어도 99% 동일하다. 일부 실시예에서, 상기 2A 펩타이드 변이체는 N-말단에 부가적인 아미노산 서열 (예, GSG)을 포함한다.
특정 실시예에서, 상기 F2A 펩타이드 서열은 서열번호 15 또는 서열번호 19의 아미노산 서열과 적어도 80%, 적어도 85%, 적어도 90%, 적어도 95%, 적어도 96%, 적어도 97%, 적어도 98% 또는 적어도 99% 동일하다. 특정 실시예에서, 상기 E2A 펩타이드 서열은 서열번호 16 또는 서열번호 20의 아미노산 서열과 적어도 80%, 적어도 85%, 적어도 90%, 적어도 95%, 적어도 96%, 적어도 97%, 적어도 98%, 또는 적어도 99% 동일하다. 특정 실시예에서, 상기 T2A 펩타이드 서열은 서열번호 17 또는 서열번호 21의 아미노산 서열과 적어도 80%, 적어도 85%, 적어도 90%, 적어도 95%, 적어도 97%, 적어도 98% 또는 적어도 99% 동일하다. 구체적인 비-제한적인 실시예에서, 상기 자가-절단 펩타이드는 서열번호 14 내지 21 중 어느 하나의 아미노산 서열을(로) 포함하거나 구성된다.
일부 실시양태에서, 상기 아데노바이러스는 아데노바이러스 5형 (Ad5)이다. 다른 실시양태에서, 상기 아데노바이러스는 Ad2, Ad3, Ad9, Ad11, Ad12 또는 Ad34이다. 또 다른 실시양태에서, 상기 아데노바이러스는 키메라 아데노바이러스, 예를 들어 Ad5/Ad9 또는 Ad5/Ad34 키메라 아데노바이러스이나, 이에 제한되지 않는다.
본원에 개시된 재조합 아데노바이러스 게놈을 포함하는 재조합 아데노바이러스가 본원에 추가로 제공된다.
본원에서 제공되는 재조합 아데노바이러스 (및 재조합 아데노바이러스 게놈)는 예를 들어 특정 세포 유형으로 상기 바이러스를 표적화하고, 간에서의 표적화 및 복제를 억제하고, 종양 세포에서 선택적 복제를 허용하고, 일반적인 아데노바이러스 항원형에 대해 기존의 중화 항체를 회피하는 추가적인 변형을 선택적으로 포함한다. 상기 추가적인 변형은 상기 재조합 아데노바이러스의 바람직한 용도에 따라 달라질 수 있다. 아데노바이러스 변형은 예를 들어, PCT 출원번호 PCT/US2015/051745 (2015년 9월 23일 출원), WO 2012/024350, WO 2013/138505 및 WO 2014/153204에 기술되어 있고, 이는 본원에 그 전체가 참고 문헌으로 인용된다.
재조합 아데노바이러스 게놈, 또는 재조합 아데노바이러스 및 약학적으로 허용 가능한 담체를 포함하는 조성물 또한 본 개시에 의해 제공된다.
또한, 치료용 단백질을 대상에게 전달하는 방법이 본원에 제공된다. 일부 실시양태에서, 상기 방법은 본원에 개시된 재조합 아데노바이러스 게놈, 재조합 아데노바이러스 또는 조성물을 투여하는 단계를 포함한다. 이러한 방법에 있어서, 상기 재조합 아데노바이러스 또는 재조합 아데노바이러스 게놈의 이종의 ORF는 상기 치료용 단백질을 암호화한다.
종양 세포 생존율 및/또는 종양 세포 성장을 감소 또는 억제(예를 들어 상기 개시된 치료법의 부재와 비교하여, 적어도 10%, 적어도 20%, 적어도 30%, 적어도 40%, 적어도 50%, 적어도 75%, 적어도 90%, 적어도 95%, 또는 적어도 98%의 감소)하는 방법이 추가로 제공된다. 일부 실시양태에서, 상기 방법은 본원에 개시된 재조합 아데노바이러스 게놈, 재조합 아데노바이러스, 또는 조성물과 종양 세포를 접촉시키는 단계를 포함한다. 이러한 방법에서, 상기 이종의 ORF는 치료용 단백질을 암호화한다. 일부 실시예에서, 상기 방법은 시험관 내(in vitro) 방법이다. 다른 실시예에서, 상기 방법은 생체 내(in vivo) 방법이고 종양 세포를 접촉시키는 단계는 상기 재조합 아데노바이러스 게놈, 재조합 아데노바이러스, 또는 조성물을 종양을 가진 대상에게 투여하는 단계를 포함한다.
종양 진행을 감소 또는 억제하는 방법, 예를 들어 대상에서 전이의 수 및/또는 크기를 감소시키거나 종양 부피를 감소시키는 방법(예를 들어 상기 개시된 치료법의 부재와 비교하여, 적어도 10%, 적어도 20%, 적어도 30%, 적어도 40%, 적어도 50%, 적어도 75%, 적어도 90%, 적어도 95%, 또는 적어도 98%의 감소)이 본원에 추가로 제공된다. 일부 실시양태에서, 상기 방법은 본원에 개시된 재조합 아데노바이러스 게놈, 재조합 아데노바이러스 또는 조성물의 치료학적으로 유효한 양을 대상에게 투여하는 단계를 포함한다. 이 방법에 있어서, 상기 이종의 ORF는 치료용 단백질을 암호화한다.
대상에서 암을 치료하는 방법이 추가로 제공된다. 일부 실시양태에서, 상기 방법은 본원에 개시된 재조합 아데노바이러스 게놈, 재조합 아데노바이러스, 또는 조성물을 대상에게 투여하는 단계를 포함한다. 이 방법에 있어서, 상기 이종의 ORF는 치료용 단백질을 암호화한다.
본원의 일부 실시양태에서, 상기 방법은 추가 치료용 제제를 대상에게 투여하는 단계를 추가로 포함한다. 예를 들어, 상기 추가 치료용 제제는 항암제, 예를 들어 화학요법 제제(chemotherapeutic agent), 생물학적 (예를 들어 항체 또는 이의 단편, 예를 들어 모노클로날 항체), 또는 핵산 분자, 예를 들어 억제성 핵산 분자) 또는 다른 치료법의 치료, 예를 들어 종양의 외과적 절제술 또는 종양의 방사선 조사를 포함할 수 있다.
또한, 본원에 개시된 재조합 아데노바이러스 게놈, 재조합 바이러스, 또는 조성물; 및 하나 이상의 추가적인 치료용 제제 및/또는 하나 이상의 진단 제제를 포함하는 키트가 본원에서 제공된다. 일부 실시양태에서, 상기 하나 이상의 추가적인 치료용 제제는 화학요법(chemotherapeutic), 생물학적(biologic), 또는 이의 조합을 포함한다. 일부 실시예에서, 상기 하나 이상의 진단 제제는 종양 마커에 특이적인 하나 이상의 항체 또는 핵산 분자, 또는 시험관 내(in vitro) 또는 생체 내(in vivo)에서 상기 바이러스 또는 종양 세포를 추적하는데 사용될 수 있는 이미징 프로브를 포함한다.
IV. 외인성 ORF의 최적 배치
36kb의 아데노바이러스 게놈은 작고, 다양한 유전자의 코딩을 위해 상단 및 하단 가닥 모두를 사용한다. 아데노바이러스 게놈 내의 많은 위치에서, 상부 및 하부 가닥 모두가 개별 유전자 암호화를 위해 동시에 사용된다. 상기 게놈 크기는 캡시드에 삽입하기에 최적으로 진화했다. 결과적으로, 상기 외인성 핵산의 과도한 첨가는 캡시드 내로의 불완전한 게놈 로딩 및 바이러스 동역학의 감소를 유도하므로, 상기 외인성 유전자의 삽입은 캡시드의 크기 용량에 의해 제한된다.
아데노바이러스 게놈에서 제한된 사용 가능한 공간에 의해 나타나는 과제에 대한 해결책은 천연 아데노바이러스 ORF 내에서 융합 생성물로서 외인성 오픈 리딩 프레임 (ORF)을 위치시키는 것이다. 이 전략은 게놈에서 이미 암호화된 아데노바이러스 프로모터, 5'UTR 및 polyA 테일을 사용한다. 그러나, 천연 아데노바이러스 단백질과 외인성 단백질 사이에서 융합의 발현은 하나 또는 두 단백질 기능에 해로울 수 있으며, 아데노바이러스 복제 동역학을 현저히 감소시킬 수 있다.
본 개시는 천연 (외인성) ORF와 외인성 (이종의) ORF 사이에 배치된 자가-절단 펩타이드 서열을 사용함으로써 이 문제에 대한 해결책을 제공한다. 단일 mRNA 상에 있는 두 개의 ORF 사이에 위치할 때, 상기 자가-절단 펩타이드 서열의 존재는 리보솜 건너뛰기(skipping) 및 제2 단백질로부터 분리된 제1 단밸질의 방출을 초래한다. 본원에 개시된 일부 실시양태에서, 상기 자가-절단 펩타이드는 2A 펩타이드(P2A)이다.
또한 상기 아데노바이러스 게놈 내 이종의 ORF에 대한 최적 배치 위치의 확인이 본원에 개시된다. 상기 자가-절단 펩타이드 서열 및 상기 이종의 ORF의 신중한(judicious) 배치는 높은 발현을 유도하고 바이러스 동역학에 최소한의 영향을 미치지 않는다.
하기 실시예 1에 기재된 바와 같이, 아데노바이러스 복제 동역학을 억제하지 않는 이종성 ORF의 삽입시, 아데노바이러스 게놈 내 몇몇 부위들이 확인되었다. 특히, 이종의 ORF는 E1B-55k ORF의 C-말단, DNA 중합효소 ORF의 N-말단, DBP ORF의 C-말단, ADP ORF의 N-말단, E3-14.7k ORF의 C-말단 또는 E4-ORF2의 N-말단에 삽입될 수 있는 것으로 확인되었다. 각 경우에, 자가-절단 펩타이드 서열 (P2A 부위)은 상기 아데노바이러스 ORF와 상기 이종의 ORF 사이에 삽입되었다. 파이버의 C-말단에 이종의 ORF를 삽입하는 것은 복제 결함이 있는 아데노바이러스를 생산함이 본원에 추가로 개시된다; 그러나, 상기 재조합 바이러스는 감염된 세포에서 매우 높은 수준의 이종의 단백질을 생성할 수 있었으며, 이는 많은 치료법의 적용에서 유용함을 증명할 수 있었다.
따라서, 본 개시는 치료법의 적용 (여기서 "SC"는 P2A와 같은 자가-절단 펩타이드를 암호화하는 서열을 지칭함)을 위해 하기의 재조합 아데노바이러스의 용도를 고려한다:
E1B-55k-SC-이종의 ORF
이종의 ORF-SC-(DNA 중합효소)
DBP-SC-이종의 ORF
이종의 ORF-SC-ADP
E3-14.7k-SC-이종의 ORF
이종의 ORF-SC-E4-ORF2
파이버-SC-이종의 ORF
본원의 일부 실시양태에서, 상기 자가-절단 펩타이드는 바이러스성 암호화된 2A 펩타이드이거나 하기에 더 기술된 바와 같이 이의 변형된 버전이다.
V. 자가-절단 펩티드 서열
자가-절단 펩타이드는 C-말단에서 펩타이드 결합의 합성을 건너뛰는 리보솜을 유도하는 펩타이드이며, 이는 상기 펩타이드 서열과 하류 폴리펩타이드의 분리를 유도한다. 자기-절단 펩타이드의 사용은 단일 ORF로부터 자가-절단 펩타이드를 플랭킹(flanking)하는 다수의 단백질의 발현을 허용한다. 바이러스성 암호화된 2A 펩타이드는 자가-절단 펩타이드의 한 유형이다.
다른 자가-절단 펩타이드와 마찬가지로, 2A 펩타이드는 리보솜이 2A 요소의 C 말단에서 펩타이드 결합의 합성을 건너뛰게 하는 기능을 하여, 2A 서열의 말단과 하류 펩타이드 사이의 분리를 유도한다 (Kim 등, PLoS One 6(4):e18556, 2011). "절단"은 2A 펩타이드의 C- 말단에 있는 글리신과 프롤린 잔기 사이에서 발생한다. 예시적인 2A 펩타이드는 토세어 아시그나 바이러스 (TaV), 마 비염 A 바이러스 (ERAV), 돼지 테스코바이러스-1 (PTV1) 및 수족구병 바이러스 (FMDV)에 의해 암호호되는 2A 펩타이드 또는 이의 변형된 버전들을 포함하나, 이에 한정되지 않는다.
본원의 특정 실시예에서, 상기 2A 펩타이드는 PTV1 2A (P2A), FMDV 2A (F2A), ERAV 2A (E2A) 또는 TaV 2A (T2A)를 포함하며, 이들의 서열은 하기에 나타내며 서열번호 14-17로 기재된다.
P2A: ATNFSLLKQAGDVEENPGP (서열번호 14)
F2A: VKQTLNFDLLKLAGDVESNPGP (서열번호 15)
E2A: QCTNYALLKLAGDVESNPGP (서열번호 16)
T2A: EGRGSLLTCGDVEENPGP (서열번호 17)
일부 실시예에서, 상기 2A 펩타이드는 절단 효율을 향상시키기 위해 N-말단에 Gly-Ser-Gly를 포함하도록 변형된다. 변형된 P2A, F2A, E2A 및 T2A의 서열을 하기에 나타내었으며 서열번호 18-21로 본원에 기재된다.
변형된 P2A: GSGATNFSLLKQAGDVEENPGP (서열번호 18)
변형된 F2A: GSGVKQTLNFDLLKLAGDVESNPGP (서열번호 19)
변형된 E2A: GSGQCTNYALLKLAGDVESNPGP (서열번호 20)
변형된 T2A: GSGEGRGSLLTCGDVEENPGP (서열번호 21)
일부 실시양태에서, 상기 2A 폴리펩타이드는 본원에 개시된 2A 펩타이드의 변이체이다. 변이체는 본원에 개시된 야생형 또는 변형된 2A 폴리펩타이드와 적어도 80%, 적어도 85%, 적어도 90%, 적어도 95%, 적어도 96%, 적어도 97%, 적어도 98%, 적어도 99%, 그 이상의 서열 상동성을 갖는 폴리펩타이드 서열을 포함할 수 있다. 변이체는 예를 들어, 서열번호 14-21 중 임의의 하나의 2A 폴리펩타이드로부터 적어도 하나의 N-말단 아미노산의 결실, 예를 들어 열거된 값 중 임의의 2개 사이의 범위를 포함하는 1, 2, 3, 4 또는 5개의 아미노산의 결실을 포함할 수 있다. 변이체는 서열번호 14-21 중 임의의 하나의 2A 폴리펩타이드로부터 적어도 하나의 C-말단 아미노산의 결실, 예를 들어 열거된 값 중 임의의 2개 사이의 범위를 포함하는 1, 2, 3, 4 또느 5개의 아미노산의 결실을 포함할 수 있다. 변이체는 예를 들어, 적어도 보존적 아미노산 치환과 같은 1, 2, 3, 4 또는 5개의 아미노산 치환을 또한 포함할 수 있다.
VI. 약학적 조성물
재조합 아데노바이러스 또는 재조합 아데노바이러스 게놈을 포함하는 조성물이 본원에서 제공된다. 상기 조성물은 시험관 내에서(in vitro) 또는 생체 내에서(in vivo), 선택적으로 제제(formulation) 및 투여에 적합하다. 선택적으로, 상기 조성물은 하나 이상의 제공되는 제제(agent) 및 약학적으로 허용 가능한 담체를 포함한다. 적합한 담체 및 이의 제제(formulation)는 Remington: The Science and Practice of Pharmacy, 22 nd Edition, Loyd V. Allen et al., editors, Pharmaceutical Press (2012)에 기술되어 있다. 약학적으로 허용 가능한 담체는 생물학적으로 또는 그렇지 않으면 바람직하지 않은 물질을 포함한다. 즉, 상기 물질은 바람직하지 않은 생물학적 효과를 일으키지 않거나 포함되어 있는 약학적 조성물의 다른 성분과 해로운 방식으로 상호작용하지 않고 대상에 투여된다. 대상에게 투여된 경우, 상기 담체는 선택적으로 활성 성분의 분해를 최소화하고 대상에서 부작용을 최소화하도록 선별된다.
재조합 바이러스 (또는 재조합 아데노바이러스를 암호화하는 하나 이상의 핵산 또는 벡터)는 공지된 방법에 따라, 예를 들어 볼루스(bolus) 또는 일정 시간 동안의 연속적인 주입에 의한 정맥내 투여(intravenous administration), 근육 내(intramuscular), 복강 내(intraperitoneal), 뇌척수 내(intracerobrospinal), 피하(subcutaneous), 관절 내(intra-articular), 활액막 내(intrasynovial), 척수강내(intrathecal), 경구(oral), 국소(topical), 종양 내(intratumoral) 또는 흡입(inhalation) 경로에 의한 공지된 방법에 따라 투여된다. 투여는 국소적 또는 전신일 수 있다. 상기 조성물은 국소, 경구, 비경구, 정맥 내, 관절 내, 복강 내, 근육 내, 피하, 강 내(intracavity), 경피흡수, 간 내(intrahepatically), 두개 내(intracranially), 분무(nebulization)/ 흡입(inhalation)을 포함하는 몇몇 임의의 투여 경로 또는 내시경을 통한 설치에 의해 투여될 수 있다. 따라서, 상기 조성물은 국소 또는 전신 치료가 바람직한지의 여부 및 치료할 영역에 따라 많은 방법으로 투여된다.
일부 실시양태에서, 투여를 위한 상기 조성물은 약학적으로 허용 가능한 담체, 바람직하게는 수성 담체에 용해된 본원에 개시된 재조합 아데노바이러스 (또는 재조합 게놈)를 포함할 것이다. 다양한 수성 담체, 예를 들어 완충 식염수 등을 사용할 수 있다. 이러한 용액은 멸균되고 일반적으로 바람직하지 않은 물질이 없다. 이러한 조성물은 통상의 잘 알려진 멸균 기술에 의해 멸균될 수 있다. 상기 조성물은 pH 조절제 및 완충제, 독성 조절제 등과 같은 생리학적 조건을 근사화하는데 필요한 약학적으로 허용 가능한 보조 물질, 예를 들어 아세트산 나트륨(sodium acetate), 염화 나트륨(sodium chloride), 염화 칼슘(potassium chloride), 염화 칼슘(calcium chloride), 락트산 나트륨(sodium lactate) 등을 포함할 수 있다. 이들 제제 중의 활성제의 농도는 광범위하게 다양할 수 있으며, 선택된 특정 투여 방식 및 대상의 요구에 따라 주로 유체 부피, 점도, 체중 등에 기초하여 선택될 것이다.
약학적 제제, 특히 재조합 바이러스의 약학적 제제는 원하는 순도를 갖는 재조합 아데노바이러스 (또는 재조합 아데노바이러스를 암호화하는 하나 이상의 핵산)를 선택적인 약학적으로 허용 가능한 담체, 부형제 또는 안정화제와 혼합함으로써 제조될 수 있다. 이러한 제제는 동결건조된 제제 또는 수용액일 수 있다.
허용 가능한 담체, 부형제 또는 안정제는 사용된 용량과 농도에서 수혜자에게 독성이 없다. 허용 가능한 담체, 부형제 또는 안정제는 아세테이트(acetate), 인산염(phosphate), 구연산염(citrate) 및 기타 유기산; 산화 방지제 (예, 아스코르브산) 방부제, 저분자량 폴리펩타이드; 혈청 알부민 또는 젤라틴과 같은 단백질, 폴리비닐피롤리돈과 같은 친수성 중함체; 및 아미노산, 단당류, 이당류, 및 글루코스, 만노오스 또는 덱스트린을 포함하는 다른 탄수화물; 킬레이트제(chelating agent); 및 이온성 및 비이온성 계면활성제 (예, 폴리소르베이트); 나트륨과 같은 염-형성 반대 이온(salt-forming counter-ion); 금속 착물 (예, Zn-단백질 착물); 및/또는 비-이온성 계면활성제를 포함할 수 있다. 상기 재조합 아데노바이러스 (또는 재조합 아데노바이러스를 암호화하는 하나 이상의 핵산)는 임의의 적절한 농도의 감염 단위로 제제화될 수 있다.
경구 투여에 적합한 제제는 (a) 물, 식염수 또는 PEG 400과 같은 희석제 중에 현탁된 재조합 아데노바이러스의 유효한 양과 같은 액체 용액; (b) 액체, 고체, 과립 또는 젤라틴과 같이 활성 성분의 미리 결정된 양을 각각 포함하는 캡슐, 사셋 또는 정제; (c) 적절한 액체의 현탁액; 및 (d) 적합한 에멀젼으로 구성될 수 있다. 정제 형태는 하나 이상의 락토오스, 수크로스, 만니톨, 소르비톨, 인산 칼슘, 옥수수 전분, 감자 전분, 미세 결정질 셀룰로오스, 젤라틴, 콜로이드성 이산화 규소, 활석, 마그네슘 스테아레이트, 스테아린산 및 기타 부형제, 착색제, 충전제, 바인더, 희석제, 완충제, 습윤제, 방부제, 향료, 염료, 붕해제 및 약학적으로 호환되는 담체를 포함할 수 있다. 로젠지(lozenge) 형태는 활성 성분 이외에, 당업계에 알려진 담체를 포함하는 젤라틴 및 글리세린 또는 수크로스 및 아카시아 에멀젼, 겔 등과 같이 불활성 염기 내 활성 성분을 포함하는 캔디 뿐만 아니라 수크로스와 같이 향료 내 활성 성분을 포함할 수 있다.
단독으로 또는 다른 적합한 성분과 조합된 재조합 아데노바이러스 (또는 재조합 아데노바이러스를 암호화하는 하나 이상의 핵산)는 흡입을 통해 투여될 수 있는 에어로졸 제제로 제조될 수 있다 (즉, 이들은 "분무될 수 있다"). 에어로졸 제제는 디클로로디플루오로메탄(dichlorodifluoromethane), 프로판(propane), 질소(nitrogen) 등과 같은 가압된 허용 가능한 추진제에 배치될 수 있다.
관절 내 (관절(joint) 내), 정맥 내, 근육 내, 종양 내, 피 내, 복강 내 및 피하 경로와 같은 비경구 투여에 적합한 제제는 수성 및 비-수성, 항산화제, 완충액, 정균제 및 의도된 수혜자의 혈액으로 제제를 등장성으로 만드는 용질(solute)을 포함하는 등장성 멸균 주사 용액, 및 현탁화제, 용해제, 증점제, 안정화제 및 방부제를 포함할 수있는 수성 및 비-수성 멸균 현탁액을 포함한다. 제공된 방법에서, 조성물은 예를 들어, 정맥 내 주입, 경구, 국소, 복강 내, 방광 내, 종양 내 또는 척수강 내(intrathecally)로 투여될 수 있다. 비경구 투여, 종양 내 투여 및 정맥 내 투여가 바람직한 투여 방법이다. 화합물 제제는 앰플 및 바이알과 같은 단위-용량 또는 다중-용량 밀폐 용기로 제공될 수 있다.
주사 용액 및 현탁액은 전술한 종류의 멸균 분말, 과립 및 정제로부터 제조될 수 있다. 생체 외 치료를 위해 아데노바이러스에 의해 형질도입되거나 형질감염되거나 핵산으로 형질감염된 세포는 전술한 바와 같이 정맥 내 또는 비경구 투여될 수 있다.
일부 실시예에서, 상기 약학적 조제(preparation)는 단위-투약 형태이다. 이러한 형태에서, 조제는 적절한 양의 활성 성분을 함유하는 단위 투여량으로 세분된다. 따라서, 약학적 조성물은 투여 방법에 따라 다양한 단위 투여 형으로 투여될 수 있다. 예를 들어, 경구 투여에 적합한 단위 투약 형태는 분말, 정제, 환제, 캡슐 및 로젠지를 포함하나, 이에 한정되지 않는다.
VII. 치료 방법
본원에 개시된 상기 재조합 아데노바이러스, 재조합 아데노바이러스 게놈 및 조성물은 치료 또는 예방 치료를 위해 투여될 수 있다. 특히, 대상 내 종양 세포 생존율 또는 성장을 감소 또는 억제, 대상 내 종양 진행을 감소 또는 억제, 전이 수를 감소, 전이의 크기 및/또는 부피를 감소, 대상 내 종양 크기 및/또는 부피를 감소 및/또는 대상 내 암을 치료하는 방법이 제공된다. 상기 방법은 재조합 아데노바이러스 또는 재조합 아데노바이러스 게놈 (또는 이의 조성물)의 치료학적으로 유효한 양을 대상에게 투여하는 단계를 포함한다. 전체적으로 기술된 바와 같이, 상기 아데노바이러스 또는 약학적 조성물은 정맥 내, 혈관 내, 척수강 내 근육 내, 피하, 종양 내, 복강 내 또는 구강을 포함하는 임의의 많은 방법으로 투여된다. 선택적으로, 상기 방법은 화학요법 제제, 생물학적 및/또는 방사선과 같은 하나 이상의 추가적인 치료제를 대상에게 투여하는 단계를 추가로 포함한다.
일부 실시양태에서, 암 또는 종양은 폐, 전립선, 결장 직장, 유방, 갑상선, 신장, 췌장, 뼈, 두경부 또는 간 암 또는 종양이거나 또는 일종의 백혈병이다. 일부 경우에서, 상기 암은 전이성이다. 일부 실시예에서, 종양은 유방(mammary), 뇌하수체(pituitary), 갑상선(thyroid) 또는 전립선(prostate gland)의 종양; 뇌(brain), 간(liver), 뇌막(meninges), 뼈(bone), 난소(ovary), 자궁(uterus) 또는 자궁 경부(cervix)의 종양; 단핵 세포(monocytic) 또는 골수성 백혈병(myelogenous leukemia); 선암(adenocarcinoma), 선종(adenoma), 별아교세포종(astrocytoma), 방광 종양(bladder tumor), 뇌 종양(brain tumor), 버킷 림프종(Burkitt’s lymphoma), 유방암(breast carcinoma), 자궁경부암(cervical carcinoma), 결장암(colon carcinoma), 신장암(kidney carcinoma), 간암(liver carcinoma), 폐암(lung carcinoma), 난소암(ovarian carcinoma), 췌장암(pancreatic carcinoma), 전립선암(prostate carcinoma), 직장암(rectal carcinoma), 피부암(skin carcinoma), 위암(stomach carcinoma), 고환암(testis carcinoma), 갑상선암(thyroid carcinoma), 연골 육종(chondrosarcoma), 융모암(choriocarcinoma), 섬유종(fibroma), 섬유육종(fibrosarcoma), 교모세포종(glioblastoma), 신경교종(glioma), 간종양(hepatoma), 조직구종(histiocytoma), 평활근모세포종(leiomyoblastoma), 평활근육종(leiomyosarcoma), 림프종(lymphoma), 지방육종 세포(liposarcoma cell), 유방 육종(mammary tumor), 수모세포종(medulloblastoma), 골수종(myeloma), 형질세포종(plasmacytoma), 신경아세포종(neuroblastoma), 신경아교종(neuroglioma), 골육종(osteogenic sarcoma), 췌장 종양(pancreatic tumor), 뇌하수체 종양(pituitary tumor), 망막모세포종(retinoblastoma), 횡문근육종(rhabdomyosarcoma), 육종(sarcoma), 고환 종양(testicular tumor), 흉선종(thymoma) 또는 윌름스 종양(Wilms tumor)이다. 종양은 혈관종(hemangiomas), 흑색종, 육종(카포시 육종(Kaposi's sarcoma) 뿐만 아니라 뼈 및 연조직에서 발생하는 것 포함) 및 뇌, 신경, 눈 및 뇌막(성상 세포종(astrocytomas), 신경 교종(gliomas), 교모세포종(glioblastomas), 망막아종(retinoblastomas), 신경종(neuromas), 신경아세포종(neuroblastomas), 신경초종(Schwannomas) 및 수막종(meningiomas)을 포함)의 종양 뿐만 아니라 유방, 결장, 직장, 폐, 구강인두(oropharynx), 하인두(hypopharynx), 식도(esophagus), 위, 췌장, 간, 담낭 및 담관(gallbladder and bile duct), 소장, 요로 (신장, 방광 및 요로상피 포함), 여성 생식기 관(융모암(choriocarcinoma) 및 임신영양막 질환(gestational trophoblastic disease) 뿐만 아니라 경부, 자궁 및 난소를 포함), 남성 생식기 관(전립성, 정낭, 고환 및 세균 세포 종양 포함), 내분비샘(갑상선, 부신 및 뇌하수체 포함), 및 피부의 암종을 포함하는 원발성 및 전이성 고형 종양을 모두 포함한다.
치료법의 적용에서, 재조합 아데노바이러스 또는 이의 조성물은 대상에게 치료학적으로 유효한 양 또는 용량으로 투여된다. 이 사용에 효과적인 양은 질병의 중증도와 환자의 건강 상태에 따라 다르다. 상기 조성물의 단일 또는 다중 투여는 환자에 의해 요구되고 허용되는 투여량 및 빈도에 따라 투여될 수 있다. "환자" 또는 "대상"은 사람과 다른 동물, 특히 포유동물을 포함한다. 따라서, 상기 방법은 인간 치료법 및 마우스, 랫트, 토끼, 고양이, 개, 소, 말, 돼지, 닭 등과 같은 수의학 적용 모두에 적용 가능하다.
변형된 서열을 갖는 아데노바이러스의 유효한 양은 개별적으로 결정되며 사용되는 특정 재조합 아데노바이러스; 개인의 크기, 나이, 성별; 및 증식하는 세포의 크기 및 다른 특성에 적어도 부분적으로 기초한다. 예를 들어, 인간의 치료를 위해, 적어도 104, 적어도 105, 적어도 106, 적어도 107, 적어도 108, 적어도 109, 적어도 1010, 1011, 또는 적어도 1012와 같이 적어도 103 플라크 형성 단위(plaque forming units, PFU)의 재조합 바이러스가 사용되고, 예를 들어 대략 103 내지 1012 PFU의 재조합 바이러스가 존재하는 증식 세포 또는 신생물(neoplasms)의 유형, 크기 및 수에 따라 사용된다. 유효량은 약 1.0 pfu/kg 체중 내지 약 1015 pfu/kg 체중 (예를 들어, 약 102 pfu/kg 체중 내지 약 1013 pfu/kg 체중) 일 수 있다. 재조합 아데노바이러스는 단일 투여량 또는 다중 투여량 (예를 들어, 2, 3, 4, 6회 또는 그 이상의 투여량)으로 투여된다. 다중 투여량을 동시에 또는 연속적으로 투여할 수 있다 (예를 들어, 며칠 또는 수주의 기간 동안).
일부 실시양태에서, 상기 제공되는 방법은 항암제 또는 다른 치료법의 치료 (예, 종양의 외과적 절제)와 같이 하나 이상의 추가적인 치료제를 대상에게 투여하는 단계를 포함한다. 예시적인 항암제는 예를 들어, 유사분열 억제제(mitotic inhibitor), 알킬화제(alkylating agent), 항 대사물질(anti-metabolite), 삽입성 항생제(intercalating antibiotics), 성장 인자 억제제(growth factor inhibitor), 세포 주기 억제제(cell cycle inhibitor), 효소(enzyme), 토포아이소머라제 억제제(topoisomerase inhibitor), 항생제(anti-survival agent), 생물 반응 조절 물질(biological response modifier), 항 호르몬(anti-hormone, 예를 들어, 항-안드로겐(anti-androgen)), 항 혈관신생제(anti-angiogenesis agent) 및 CDK 억제제(CDK inhibitor)와 같은 화학요법 제제를 포함하나, 제한되지 않는다. 다른 항암 치료는 방사선 치료 및 암세포를 특이적으로 표적화하는 다른 항체를 포함한다.
알킬화제의 비-제한적인 예로는 니트로젠 머스타드(nitrogen mustards) (예를 들어, 메클로레타민(mechlorethamine), 시클로포스파미드(cyclophosphamide), 멜팔란(melphalan), 우라실 머스타드(uracil mustard), 또는 클로람부실(chlorambucil)), 알킬 설포네이트(alkyl sulfonate) (예를 들어, 부설판(busulfan)), 니트로소요소(nitrosoureas) (예, 카르무스틴(carmustine), 로무스틴(lomustine), 세무스틴(semustine), 스트렙토조신(streptozocin), 또는 다카바진(dacarbazine))를 포함한다.
항 대사물질의 비-제한적인 예로는 엽산 유사체 (예를 들어, 메토트렉세이트(methotrexate)), 피리미딘 유사체(예를 들어, 5-FU 또는 사이타라빈(cytarabine)), 및 퓨린 유사체, 예를 들어 메르캅토퓨린(mercaptopurine) 또는 티오구아닌(thioguanine)을 포함한다.
천연물(natural product)의 비-제한적인 예로는 빈카 알칼로이드(vinca alkaloids) (예를 들어 빈블라스틴(vinblastine), 빈크리스틴(vincristine), 또는 빈데신(vindesine)), 에피포도필로톡신(epipodophyllotoxin) (예를 들어 에토포시드(etoposide) 또는 테니포시드(teniposide)), 항생제(예를 들어, 닥티노마이신(dactinomycin), 다우노루비신(daunorubicin), 독소루비신(doxorubicin), 블레오마이신(bleomycin), 플리마이신(plicamycin) 또는 미토마이신 C(mitomycin C)) 및 효소(예를 들어 L-아스파라기나제(L-asparaginase))를 포함한다.
기타 제제(miscellaneous agent)의 비-제한적인 예로는 백금 배위 착물(platinum coordination complexe) (예를 들어, 시스플라틴(cisplatin)으로 도 알려진 시스-디아민-디클로로플라티늄 II (cis-diamine-dichloroplatinum II)), 치환된 요소(예를 들어 하이드록시우레아(hydroxyurea)), 메틸 하이드라진 유도체(예를 들어, 프로카바진(procarbazine)) 및 부신피질 억제제 (예를 들어 마이토테인(mitotane) 및 아미노글루테티미드(aminoglutethimide))를 포함한다.
호르몬 및 길항제(antagonist)의 비-제한적인 예로는 부신피질 스테로이드(adrenocorticosteroid) (예를 들어 프리드니손(prednisone)), 프로게스틴(progestin) (예를 들어 하이드록시프로게스테론 카프로에이트(hydroxyprogesterone caproate), 메드록시프로게스테론 아세테이트(medroxyprogesterone acetate) 및 메게스트롤 아세테이트(magestrol acetate)), 에스트로겐(estrogen) (예를 들어 디에틸스틸베스트롤(diethylstilbestrol) 및 에티닐 에스트라디올(ethinyl estradiol)), 항에스트로젠(antiestrogen) (예를 들어 테스테론 프로프리오네이트(testerone proprionate) and 플루옥시메스테론(fluoxymesterone))을 포함한다.
가장 일반적으로 사용되는 화학요법 약물의 예는 아드리아마이신(Adriamycin), 알케란(Alkeran), 아라-C(Ara-C), 비아이씨엔유(BiCNU), 부설판( Busulfan), CCNU, 카보플라티늄(Carboplatinum), 시스플라티늄(Cisplatinum), 사이톡산(Cytoxan), 다우노루비신(Daunorubicin), 다카바진(DTIC), 5-플루오로우라실(5-FU), 플루다라빈(Fludarabine), 히드리아(Hydrea), 아이다루비신(Idarubicin), 이포스파마이드(Ifosfamide), 메토트렉세이트(Methotrexate), 미스라마이신(Mithramycin), 미토마이신(Mitomycin), 미톡산트론(Mitoxantrone), 나이트로젠 머스타드(Nitrogen Mustard), 택솔(Taxol) (또는 다른 탁산, 예를 들어 도세탁셀(docetaxel)), 벨반(Velban), 빈크리스틴(Vincristine), VP-16을 포함하며, 일부 더 새로운 약물은 젬시타빈(Gemcitabine) (젬자주(Gemzar)), 허셉틴(Herceptin), 이리노테칸(Irinotecan) (캠토사(Camptosar), CPT-11), 류스타틴(Leustatin), 나벨빈(Navelbine), 리툭산(Rituxan) STI-571, 탁소텔(Taxotere), 토포테칸(Topotecan) (하이캄틴(Hycamtin)), 젤로다(Xeloda) (카페시타빈(Capecitabine)), 제벨린(Zevelin) 및 칼시트리올(calcitriol)을 포함한다.
사용될 수 있는 면역조절제의 비-제한적인 예로는 AS-101 (Wyeth-Ayerst Labs.), 브로피리민(bropirimine) (Upjohn), 감마 인터페론(gamma interferon) (Genentech), GM-CSF (과립구 대식세포 콜로니 자극 인자(granulocyte macrophage colony stimulating factor); Genetics Institute), IL-2 (Cetus or Hoffman-LaRoche), 인간 면역 글로불린(human immune globulin) (Cutter Biological), IMREG (from Imreg of New Orleans, La.), SK&F 106528, 및 TNF (종양 괴사 인자(tumor necrosis factor); Genentech)를 포함한다.
CDK(Cyclin-dependent kinase, 사이클린-의존성 인산화효소) 억제제는 CDK의 기능을 억제하는 제제이다. 상기 제공되는 방법에서의 사용을 위한 CDK 억제제의 비-제한적인 예는 AG-024322, AT7519, AZD5438, 플라보피리돌(flavopiridol), 인디설람(indisulam), P1446A-05, PD-0332991, 및 P276-00 (예를 들어, Lapenna et al., Nature Reviews, 8:547-566, 2009를 참조)를 포함한다. 다른 CDK 억제제는 LY2835219, 팔보시클립(Palbociclib), LEE011 (Novartis), pan-CDK 억제제 AT7519, 셀리시클립(seliciclib), CYC065, 부티로락톤 I(butyrolactone I), 하이메니알디신(hymenialdisine), SU9516, CINK4, PD0183812 또는 파스카프라이신(fascaplysin)을 포함한다.
일부 실시예에서, 상기 CDK 억제제는 광범위한 범위의 억제제(예를 들어, 플라보피리돌(flavopiridol), 올로뮤신(olomoucine), 로스코비틴(roscovitine), 켄파울론(kenpaullone), SNS-032, AT7519, AG-024322, (S)-Roscovitine 또는 R547이다. 다른 실시예에서, 상기 CDK 억제제는 특이적인 억제제(예를 들어, 파스카프라이신(fascaplysin), 류비딘(ryuvidine), 퓨르발라놀 A(purvalanol A), NU2058, BML-259, SU 9516, PD0332991 또는 P-276-00)이다.
일부 실시양태에서, 상기 제공되는 방법은 치료학적으로 유효한 양의 면역요법을 대상에게 투여하는 단계를 추가로 포함한다. 사용될 수 있는 면역조절제의 비-제한적인 예는 AS-101 (Wyeth-Ayerst Labs.), 브로피리민(bropirimine) (Upjohn), 감마 인터페론(gamma interferon) (Genentech), GM-CSF (과립구 대식세포 콜로니 자극 인자(granulocyte macrophage colony stimulating factor); Genetics Institute,) IL-2 (Cetus or Hoffman-LaRoche), 인간 면역 글로불린(human immune globulin) (Cutter Biological), IMREG (from Imreg of New Orleans, La.), SK&F 106528, 및 TNF (종양 괴사 인자(tumor necrosis factor); Genentech)를 포함한다. 면역치료제는 예를 들어, 아테졸리주맙(Atezolizumab), MPDL3280A, BNS-936558 (니볼루맙(Nivolumab)), 펨브로리주맙(Pembrolizumab), 피딜리주맙(Pidilizumab), CT011, AMP-224, AMP-514, MEDI-0680, BMS-936559, BMS935559, MEDI-4736, MPDL-3280A, MSB-0010718C와 같이 PD-1 또는 PD-L1에 특이적으로 결합하는 항체와 같은 PD-1 길항체 또는 PD-L1 길항제일 수 있다. 상기 면역치료제는 트레멜리무맙(Tremelimumab), BMS-986016, 및 MGA271과 같은 CTLA-4, LAG-3, 또는 B7-H3 길항제일 수 있다.
일부 실시양태에서, 상기 제공되는 방법은 하나 이상의 항-혈관신생 제제, 예를 들어 혈관 성장을 감소시키거나 심지어 억제하는 기능을 하는 단백질, 효소, 다당류, 올리고뉴클레오티드, DNA, RNA, 및 재조합 벡터 및 소분자의 치료학적으로 유효한 양을 대상에게 투여하는 단계를 추가로 포함한다. 적절한 혈관신생 억제제의 예로는 안지오스타딘 K1-3(angiostatin K1-3), 스타우로스포린(staurosporine), 제니스테인(genistein), 푸마길린(fumagillin), 메드록시프로게스테론(medroxyprogesterone), 수라민(suramin), 인터페론-알파(interferon-alpha), 금속단백분해효소 억제제(metalloproteinase inhibitor), 혈소판 인자 4(platelet factor 4), 소마토스타틴(somatostatin), 트롬보스폰딘(thromobospondin), 엔도스타틴(endostatin), 탈리도마이드(thalidomide), 및 이의 유도체 및 유사체를 포함하나, 이에 제한되지 않는다. 예를 들어, 일부 실시양태에서 상기 항-혈관신생 제제는 VEGF에 특이적으로 결합하는 항체 (예, AVASTIN®, Roche) 또는 VEGF 수용체에 특이적으로 결합하는 항체 (예, VEGFR2 항체)이다. 일 실시예에서, 상기 항-혈관신생 제제는 VEGFR2 항체, 또는 DMXAA (Vadimezan 또는 ASA404로도 알려짐; 예를 들어 Sigma 사로부터 상업적으로 이용 가능함, St. Louis, MO) 또는 모두를 포함한다. 상기 항-혈관신생 제제는 베바시주맵(bevacizumab), 서니티닙(sunitinib), 서니티닙, 시티닙(xitinib), 다사티닙(dasatinib)과 같은 항-혈관신생 티로신 인산화 억제제(tyrosine kinase inhibitors (TKI))일 수 있다. 이들은 개별적으로 또는 임의의 조합으로 사용될 수 있다.
일부 실시양태에서, 상기 제공되는 방법은 하나 이상의 인산화 억제제, 예를 들어 성장 인자의 인산화 및 활성화를 방지하는 글리백®(Gleevac®), 이레사®(Iressa®), 및 타세바®(Tarceva®), 서니티닙(sunitinib), 소라페닙(sorafenib), 아니티닙(anitinib), 및 다사티닙(dasatinib)의 치료학적으로 유효한 양을 대상에게 투여하는 단계를 추가로 포함한다. 사용될 수 있는 항체는 성장 인자 및 혈관신생 경로를 차단하는 허셉틴®(HERCEPTIN®) 및 아바스틴®(AVASTIN®)을 포함한다. 이들은 개별적으로 또는 임의의 조합으로 사용될 수 있다.
일부 실시양태에서, 상기 제공되는 방법은 하나 이상의 모노클로날 항체, 예를 들어, 3F8, 아바고보맙(Abagovomab), 아데카투무맙(Adecatumumab), 아푸투주맙(Afutuzumab), 알라시주맙(Alacizumab), 알렘투주맙(Alemtuzumab), 알투모맙 펜테테이트(Altumomab pentetate), 아나투모맙 마페나톡스(Anatumomab mafenatox), 아폴리주맙(Apolizumab), 아르시투모맙(Arcitumomab), 바비툭시맙(Bavituximab), 베크투모맙(Bectumomab), 벨리무맙(Belimumab), 베실레소맙(Besilesomab), 베바시주맙(Bevacizumab), 비바투즈맙 메르탄신(Bivatuzumab mertansine), 블리나투모맙(Blinatumomab), 브렌툭시맙 베도틴(Brentuximab vedotin), 칸투주맙 메르탄신(Cantuzumab mertansine), 카르포맙 펜디타이드(Capromab pendetide), 카투마소맙(Catumaxomab), CC49, 세툭시맙(Cetuximab), 시타투주맙 보가톡스(Citatuzumab bogatox), 시수투무맙(Cixutumumab), 클리바투주맙 테트락세탄(Clivatuzumab tetraxetan), 코나투무맙(Conatumumab), 다세투주맙(Dacetuzumab), 데투모맙(Detumomab), 에크로멕시맙(Ecromeximab), 에큐리주맙(Eculizumab), 에드레콜로맙(Edrecolomab), 에프라투주맙(Epratuzumab), 에르투막소맙(Ertumaxomab), 에타라시주맙(Etaracizumab), 파를레투주맙(Farletuzumab), 피기투무맙(Figitumumab), 갈릭시맙(Galiximab), 젬투주맙 오조가미신(Gemtuzumab ozogamicin), 기렌툭시맙(Girentuximab), 글렘바투무맙 베도틴(Glembatumumab vedotin), 이브리투모맙 튜세탄(Ibritumomab tiuxetan), 이고보맙(Igovomab), 임시로맙(Imciromab), 인테투무맙(Intetumumab), 이노투주맙 오조가미신(Inotuzumab ozogamicin), 이필리무맙(Ipilimumab), 이라투무맙(Iratumumab), 라베투주맙(Labetuzumab), 렉사투무맙(Lexatumumab), 린투주맙(Lintuzumab), 로보투주맙 메르탄신(Lorvotuzumab mertansine), 루카투무맙(Lucatumumab), 루밀릭시맙(Lumiliximab), 마파투무맙(Mapatumumab), 마투주맙(Matuzumab), 메폴리주맙(Mepolizumab), 메텔리무맙(Metelimumab), 밀라투주맙(Milatuzumab), 미투모맙(Mitumomab), 모롤리무맙(Morolimumab), 나콜로맙 타페나톡스(Nacolomab tafenatox), 나프투모맙 에스타페나톡스(Naptumomab estafenatox), 네시투무맙(Necitumumab), 니모투주맙(Nimotuzumab), 노페투모맙 메르펜탄(Nofetumomab merpentan), 오파투무맙(Ofatumumab), 올라라투맙(Olaratumab), 오포투주맙 모나톡스(Oportuzumab monatox), 오레고보맙(Oregovomab), 파니투무맙(Panitumumab), 펨투모맙(Pemtumomab), 페르투주맙(Pertuzumab), 핀투모맙(Pintumomab), 프리투무맙(Pritumumab), 라무시루맙(Ramucirumab), 리로투무맙(Rilotumumab), 리툭시맙(Rituximab), 로바투무맙(Robatumumab), 사투모맙 펜데타이드(Satumomab pendetide), 시브로투주맙(Sibrotuzumab), 소넵시주맙(Sonepcizumab), 타카투주맙 테트라세탄(Tacatuzumab tetraxetan), 타플리투모맙 팝톡스(Taplitumomab paptox), 테나투모맙(Tenatumomab), TGN1412, 티칠리무맙(Ticilimumab) (트레멜리무맙, tremelimumab), 티가투주맙(Tigatuzumab), TNX-650, 트라스투주맙(Trastuzumab), 트레멜리무맙(Tremelimumab), 투코투주맙 셀모루킨(Tucotuzumab celmoleukin), 벨투주맙(Veltuzumab), 볼로시지맙(Volociximab), 보투무맙(Votumumab), 또는 잘루투무맙(Zalutumumab)의 치료학적으로 유효한 양을 대상에게 투여하는 단계를 추가로 포함한다. 일부 실시예에서, 상기 이종의 ORF는 이들 치료용 항체 중 하나를 암호화한다.
일부 유형의 암을 위한 또 다른 일반적인 치료는 외과적 치료, 예를 들어 암 또는 그의 일부의 외과적 절제술이다. 치료의 또 다른 예는 방사선 치료, 예를 들어 방사성 물질 또는 에너지(예를 들어, 외부 광선 요법(external beam therapy))를 종양 부위에 투여하여 외과적 절제술 이전에 종양을 근절하거나 수축시키는 것을 돕는 것이다.
제제 및 투여량의 선택은 치료되는 주어진 질환에 기초하여 당업자에 의해 용이하게 결정될 수 있다. 제제 또는 조성물의 조합은 부수적으로, 개별적으로 그러나 동시에 또는 순차적으로 투여될 수 있다. 따라서, 용어 조합은 2개 이상의 제제 또는 조성물의 부수적이거나 동시적이거나 순차적인 투여를 지칭한다.
본원에 개시된 방법에 따라, 상기 대상에게 본원에 제공되는 하나 이상의 제제의 유효한 양을 투여한다. 상기 유효한 양은 원하는 생리적 반응(예, 암 세포의 사멸)을 생산하는데 필요한 임의의 양으로 정의된다. 치료제는 전형적으로 매일 약 0.001 mg/kg 내지 약 1000 mg/kg의 초기 투여량으로 투여된다. 약 0.01 mg/kg 내지 약 500 mg/kg, 또는 약 0.1 mg/kg 내지 약 200 mg/kg, 또는 약 1 mg/kg 내지 약 100 mg/kg, 또는 약 10 mg/kg 내지 약 50 mg/kg의 투여량 범위가 사용될 수 있다. 그러나, 투여량은 대상의 필요조건, 치료되는 상태의 중증도, 및 사용되는 화합물에 따라 다양할 수 있다. 예를 들어, 투여량은 특정 대상에서 진단된 암의 유형 및 단계를 고려하여 투여량을 경험적으로 결정할 수 있다. 상기 제공되는 방법과 관련하여, 대상에게 투여되는 투여량은 시간 경과에 따라 환자에게 유익한 치료 반응에 영향을 미치기에 충분해야 한다. 특정 상황에 대한 적절한 투여량의 결정은 의사의 기술 범위 내에 있다. 따라서, 제제를 투여하기 위한 유효한 양 및 일정은 당업자에 의해 경험적으로 결저될 수 잇다. 투여량은 원하지 않는 교차-반응(cross-reaction), 아나필락시 반응(anaphylactic reaction) 등과 같은 실질적인 유해한 부작용을 유발할 정도로 커서는 안된다. 투여량은 임의 금기의 이벤트 시 개별 의사에 의해 조정될 수 있다. 가이드라인은 의약품의 주어진 부류에 적절한 투여량에 대한 문헌에서 발견될 수 있다.
본원에 개시된 바와 같이, 재조합 아데노바이러스, 재조합 아데노바이러스 게놈, 또는 이의 조성물과 종양 세포를 접촉시킴으로써 종양 세포 생존율 및/또는 성장을 억제하는 방법이 본원에 제공된다. 일부 실시양태에서, 상기 방법은 시험관 내(in vitro) 방법이다. 다른 실시양태에서, 상기 방법은 생체 내(in vivo) 방법이고 종양 세포를 접촉시키는 단계는 상기 재조합 아데노바이러스 게놈, 재조합 아데노바이러스, 또는 조성물을 종양을 가진 대상에게 투여하는 단계를 포함한다.
본원에 개시된 재조합 아데노바이러스 또는 재조합 아데노바이러스 게놈 (또는 이의 조성물)의 치료학적으로 유효한 양을 대상에게 투여함으로써, 대상의 종양 진행을 억제하거나 종양 부피를 감소시키는 방법이 추가로 제공된다.
본원에 개시된 재조합 아데노바이러스 또는 재조합 아데노바이러스 게놈 (또는 이의 조성물)의 치료학적으로 유효한 양을 대상에게 투여함으로써, 대상의 암을 치료하는 방법도 제공된다.
VIII. Adsembly 및 AdSLIC
상기 아데노바이러스 게놈은 E1, E2, E3, E4 및 L1-5로 라벨링된 여러 기능적 그룹으로 분류된다. E1 영역은 모든 다른 바이러스 유전자의 전사를 조절하고 숙주 세포에서 S-단계(phse)를 유도하는 단백질을 암호화한다. E2 영역은 바이러스 DNA 복제를 유도하는 단백질을 암호화한다. E3 영역은 숙주 세포 면역 반응을 조절하고 세포 배양액에서 불필요할 수 있는 단백질을 암호화한다. E4 영역은 서로 전혀 다른 기능의 세트에 대한 유전자를 포함한다. 그리고 L1-5 영역은 바이러스 입자 구조적 단백질을 암호화한다.
이 자연적인 기능의 분리를 이용하여, 본 발명자들은 도 6a에 도시된 바와 같이, 4개의 플라스미드, E1, E3, E4 및 코어(Core)로 분리함으로써, 36 kb Ad 게놈의 신속하고 용이한 조작을 가능하게 하는 재조합 아데노바이러스 어셈블리의 방법을 이전에 개발하였다 (Adsembly 및 AdSLIC; WO 2012/024351를 참조, 이는 본원에 참조로 통합됨). 이들의 보다 합리적인 크기 때문에, 이러한 더 작은 플라스미드의 조작은 표준 기술을 이용하여 간단하다.
Adembly 및 AdSLIC는 4시간만에 호환 가능한 게놈 라이브러리 부분으로부터 새로운 특성을 가진 아데노바이러스의 조합의 시험관 내 어셈블리를 가능하게 한다. Adsembly 및 AdSLIC는 기능적 부분의 4개의 라이브러리를 이용하여 새로운 특성을 갖는 합성 바이러스가 어셈블될 수 있게 하는 일반적인 게놈 설계 플랫폼을 제공한다 (도 6a). 이러한 부분의 라이브러리는 다중-부위 특이적 재조합 부위(Adsembly; 도 6b) 또는 서열 독립적인 심리스(seamless) 클로닝(AdSLIC; 도 6c)을 이용하여 가능한 모든 조합으로 재조합될 수 있다.
Adembly 및 AdSLIC 기술은 독특한 기능을 가진 아데노바이러스의 모듈식 설계 및 생산을 가능하게 한다. 자동화된 고-처리량(high-throughput) 방식에서 바이러스를 설계하고, 제조하고, 테스트하는 능력의 개발은 치료, 진단 및 리서치 연구를 위한 새로운 바이러스의 개발을 가속화하고 확장할 것이다.
클로닝 단계가 한 때 새로운 바이러스 컨스트럭트를 생산하기 위한 병목이었지만, Adsembly 및 AdSLIC의 출현은 그것을 만들어, 바이러스 게놈을 구축하는 능력이 그것들을 테스트하는 능력을 앞질렀다. 동등하게 고처리량 동역학 어세이는 Adsembly 및 AdSLIC 방법을 이용하여 합성 및 개인화된 바이러스 치료 및 진단의 풍부한 잠재력과 많은 내용의 어셈블리를 활용하는 데 중요하다.
하기의 실시예는 어떤 특정한 특징 및/또는 실시양태를 예시하기 위해 제공된다. 이 실시예들은 기술된 특정 특징 또는 실시양태로 본 개시를 제한하는 것으로 해석되어서는 안된다.
실시예
실시예 1: 외인성 ORF를 위한 아데노바이러스 게놈 내 최적 위치의 확인
이 실시예는 바이러스 동역학 방해없이, 자가-절단 펩타이드 서열과 함께 외인성 ORF가 삽입될 수 있는 아데노바이러스 게놈 내에서 특정 위치의 확인을 기술한다.
아데노바이러스 벡터 내 외인성 유전자의 삽입은 아데노바이러스 캡시드의 크기 용량에 의해 제한된다. 외인성 핵산의 과도한 첨가는 캡시드 내로의 불완전한 게놈 로딩 및 감소된 바이러스 동역학으로 이어진다. 아데노바이러스 게놈 내 제한된 사용 가능한 공간에 의해 제시된 과제에 대한 해결책은 천연 아데노바이러스 ORF 내에서 융합 생성물로서 외인성 오픈 리딩 프레임(ORF)을 위치시키는 것이다. 이 전략은 게놈 내 이미 암호화된 아데노바이러스 프로모터, 5'UTR 및 polyA 테일을 사용한다. 그러나, 천연 아데노바이러스 단백질과 외인성 단백질 사이의 융합체의 발현은 하나 또는 두 단백질 기능에 해로울 수 있고 아데노바이러스 복제 동역학에 현저한 감소로 이어질 수 있다. 실제로, 본원에 개시된 연구는 아데노바이러스 E1A, DNA 중합효소 또는 ADP ORF에 대한 외인성 ORF의 직접적인 융합은 아데노바이러스 복제 동역학을 현저하게 억제함을 입증한다. 또한, 본 발명자들은 이전에 내부 리보솜 도입 부위(internal ribosomal entry site, IRES)을 이용하여 외인성 ORF를 삽입하기 위해 시도했고, 이는 또한 야생형 동역학을 갖는 재조합 바이러스를 생산하지 못했다.
이 실시예는 천연 아데노바이러스 ORF 및 외인성 ORF 사이에 배치된 자가-절단 펩타이드 서열을 이용함으로써 이 문제에 대한 해결책을 설명한다. 단일 mRNA 상에 두 개의 ORF 사이에 위치할 때, 자가-절단 펩타이드 서열의 존재는 리보솜 건너뛰기 및 제2 단백질로부터 분리된 제1 단백질의 방출을 유도한다. 이 실시예에서 생성된 아데노바이러스 컨스트럭트는 이종의 ORF로서 자가-절단 펩타이드 P2A 및 형광 단백질(예, YPet, mCherry)을 이용한다.
하기의 표는 생성된 컨스트럭트의 목록을 제공하고 두 개의 다른 세포주 (293-E4 세포 및 A549 세포)에서 이종의 ORF의 발현 수준 (낮음, 중간 또는 높음) 및 바이러스 복제 동역학의 수준 (낮음, 중간 또는 높음)을 나타낸다.
Figure 112021060760163-pat00002
두 개의 세포 유형에서 "높은" 복제 동역학(즉, 야생형 아데노바이러스에 필적하는 복제 동역학)을 나타내는 컨스트럭트는 치료용 아데노바이러스 컨스트럭트(굵게 표시)를 생성하기 위한 후보 물질로 간주된다. 또한, 상기 파이버-P2A-이종의 ORF(fiber-P2A-heterologous ORF) 컨스트럭트는 바이러스 복제에서 유의한 결함을 나타냈지만, 이종의 단백질의 엄청나게 높은 발현 수준을 나타냈다. 따라서, 이러한 파이버 컨스트럭트는 또한 복제 결합이 있는 아데노바이러스의 맥락에서 치료용 단백질의 높은 수준의 생산을 위한 치료법 적용에 사용될 수 있다.
P2A 부위의 직접적인 융합 및 삽입의 비교
형광 단백질이 아데노바이러스 ORF에 직접적으로 융합된 몇몇 컨스트럭트가 생성되었다. 특히, 하기의 직접적인 융합체가 생성되었다: YPet-E1A, YPet-(DNA 중합효소(polymerase)) 및 mCherry-ADP.
YPet-E1A 아데노바이러스는 바이러스 동역학에 심각한 손상을 나타냈다. YPet과 E1A (YPet-P2A-E1A) 사이의 P2A 부위의 삽입은 바이러스 동역학을 향상시켰지만, 바이러스 동역학을 야생형 수준으로 회복하지 못했다. E1A(E1A-P2A-YPet)의 C-말단에 대한 P2A 및 YPet의 융합을 시험하기 위해 또 다른 컨스트럭트를 생성하였다. 이 컨스트럭트는 바이러스 동역학을 더 향상시켰지만, 야생형 아데노바이러스의 수준으로 동역학을 회복하지 못하였다.
YPet-(DNA-poly) 게놈 플라스미드를 형질감염시키려는 여러 시도가 생존 가능한 바이러스를 생산하지 못했다 (플라크가 형성되지 않았다). 그러나, DNA 중합효소의 N-말단에 YPet-P2A의 융합 (YPet-P2A-(DNA poly)) 상기 표에서 볼 수 있듯이 야생형 동역학을 지닌 바이러스를 생산했다.
또한, ADP에 대한 mCherry의 직접적인 융합 (mCherry-ADP)은 현저하게 손상된 동역학을 가진 바이러스를 생산했다. 그러나, mCherry ORF와 ADP ORF 사이에 P2A 부위의 삽입은 야생형 동역학을 가진 바이러스 (mCherry-P2A-ADP)를 만들었다. 다른 형광 단백질을 이용하여 동일한 결과를 얻었다; YPet-P2A-ADP 컨스트럭트는 야생형 바이러스 동역학을 나타냈다. 그러나, ADP의 C-말단 측에 P2A과 이종의 ORF의 배치는 복제되지 않은 바이러스를 생산하였다. 따라서, ADP의 경우, 이종의 ORF는 N-말단에 위치해야 한다.
파이버에 대한 직접적인 융합 및 파이버와 이종의 ORF 사이의 P2A 부위의 삽입은 모두 현저하게 손상된 복제 동역학을 가진 바이러스를 생산했다. 그러나, 파이버-P2A-이종의 ORF를 포함하는 재조합 아데노바이러스는 엄청나게 높은 이종의 단백질의 발현 수준을 나타냈다 (이 실시예에서 YPet 또는 청색 형광 단백질(BFP)).
야생형 바이러스 동역학을 가진 추가적인 컨스트럭트
도 7은 6개의 상이한 컨스트럭트의 Ln-슬로프의 비교를 나타낸다: YPet-E1A, YPet-P2A-E1A, E1A-P2A-mCherry, E1B-55k-P2A-YPet, YPet-P2A-ADP 및 Fiber-P2A-YPet. 상기 논의된 바와 같이, E1A에 대한 YPet의 직접적인 융합은 현저하게 손상된 동역학을 가진 바이러스를 생산했고, N-말단(YPet-P2A-E1A) 또는 C-말단(E1A-P2A-mCherry)에 P2A 부위의 첨가는 야생형 수준은 아니지만 바이러스 동역학을 개선했다. 그러나, E1B-55k의 C-말단(E1B-55k-P2A-YPet) 또는 ADP의 N-말단(YPet-P2A-ADP)에서의 P2A 부위 및 이종의 ORF를 삽입하는 것은 야생형 바이러스 동역학을 가진 재조합 바이러스를 생성했다.
DBP의 C-말단(DBP-P2A-YPet) 또는 E3-14.7k의 C-말단(E3-14.7k-P2A-YPet)에 P2A 부위 및 이종의 ORF를 가지거나, E4-ORF2의 N-말단 (YPet-P2A-E4-ORF2 및 mCherry-P2A-E4-ORF2)에 P2A 부위 및 이종의 ORF를 가진 컨스트럭트에 대한 바이러스 동역학의 평가는 야생형 복제 동역학을 가진 바이러스를 생산했다.
이 데이터의 결과는 적어도 하기의 아데노바이러스 게놈 컨스트럭트가 치료용 아데노바이러스 컨스트럭트를 개발하는 데에 사용될 수 있음을 입증한다:
E1B-55k-P2A-이종의 ORF
이종의 ORF-P2A-(DNA 중합효소)
DBP-SC-이종의 ORF
이종의 ORF-SC-ADP
E3-14.7k-SC-이종의 ORF
이종의 ORF-P2A-E4-ORF2
fiber-P2A-이종의 ORF
치료법의 적용을 위해, 상기 이종의 ORF는 치료용 단백질을 암호화한다.
다른 아데노바이러스 항원형
이전에 기술된 바이러스 동역학을 측정하는 방법은 모두 세포형 특이적 어세이에 크게 의존하며, 각 아데노바이러스 항원형의 발산(divergent) 향성(tropism) 때문에 항원형 특이적이다. 본원에 개시된 아데노바이러스 동역학 어세이는 임의의 하나의 세포 유형에 의존하지 않으므로 Ad5보다 다른 항원형으로 확장될 수 있다. 모든 아데노바이러스 항원형은 Ad5 E3-14.7k와 동등한 ORF를 포함한다. 따라서, Ad5 E3-14.7k-P2A-YPet (PCMN-887; 서열번호 9) 와 동등한 바이러스를 Ad9 (E3-15k을 포함) 및 Ad34 (E3-14.8k을 포함)을 이용하여 생성했다: PCMN-888 (Ad9 E3-15k-P2A-YPet; 서열번호 22) 및 PCMN-889 (Ad34 E3-14.8k-P2A-YPet; 서열번호 23). Ad5 코어 및 Ad9 또는 Ad34의 파이버 샤프트(shaft) 및 손잡이(knob)를 포함하는 키메라 바이러스도 생성되었다. 4개의 재조합 바이러스를 FBVK 어세이에서 293 세포(도 9a), A549 세포(도 9b) 및 U2OS 세포(도 9c)를 이용하여 테스트하였다. 4개의 모든 재조합 바이러스는 이종의 ORF의 삽입으로부터 인한 바이러스 동역학에 대한 최소한의 영향을 가지고 높은 수준의 YPet 발현을 나타냈다.
실시예 2: 아데노바이러스 복제 동역학을 평가하는 방법
재조합 아데노바이러스를 어셈블하기 위한 Adsembly 및 AdSLIC 방법은 단기간에 수많은 재조합 바이러스 게놈 및 바이러스를 생성하는 수단을 제공한다. 그러나, 임상적 및 치료법의 용도로 설계된 재조합 아데노바이러스의 복제 동역학을 평가하기 위한 신속하고 고-처리량 방법이 필요하다. 이 실시예는 재조합 아데노바이러스의 바이러스 복제 동역학을 시험하는데 사용될 수 있는 형광-기반의 바이러스 동역학 어세이를 설명한다 (도 3). 어세이는 재조합 아데노바이러스 게놈 플라스미드 또는 재조합 아데노바이러스 입자를 출발 물질로 하여 수행될 수 있다.
재조합 아데노바이러스 게놈으로 시작하는 경우, 상기 어세이는 세포를 아데노바이러스 게놈 플라스미드 (예, 실시예 1에서 상기 기재된 것들)로 형질감염시키고 시간에 따른 형광 발현을 모니터링하는 것을 포함한다 (도 4a-4b). 형질감염 조건은 세포의 약 5-10%가 초기에 형질감염되도록 선택된다. 초기에 형질감염되지 않은 세포는 초기 형질감염으로 생성된 바이러스 입자에 의한 2차 감염에 이용 가능하다. 로그-슬로프(Log-slope)는 2차, 3차 및 4차(등) 감염에 기초한 동력학의 척도로 사용되므로, 초기에 형질감염된 세포의 백분율을 알 필요가 없다. 도 4a 및 4b는 재조합 아데노바이러스 게놈 플라스미드로 시작하는 예시적인 바이러스-기반 동역학 어세이를 나타낸다. 이 실시예에서, 48-웰 플에이트가 사용되며, 이는 14개의 다른 바이러스 컨스트럭트(3개씩)의 검사를 동시에 가능하게 한다. 48-웰 플레이트의 상반부(도 4a)는 6개의 다른 바이러스의 3중(triplicate) 웰, 3개의 거짓(mock)-감염된 웰 및 FLUORESBRITETM 비드를 가진 3개의 "블랭크(blank)" 웰을 포gkagkua, 이는 민감성 편차를 보상한다. 48-웰 플레이트의 하반부(도 4b)는 8개의 다른 바이러스 컨스트럭트의 3중 웰을 포함한다. 일단 세포가 형질감염되면, 플레이트를 지속적인 형광 모니터링을 위한 TECANTM 플레이트 리더기에 배치한다. 수집된 데이터는 각 컨스트럭트의 ln-슬로프 계산을 위해 사용된다 (도 8).
상기 어세이는 또한 재조합 바이러스 입자로 세포를 감염시킴으로써 수행될 수 있다. 어세이의 이 버전에서, 세포를 재조합 바이러스 입자로 감염시키고 발색단 발현을 시간에 따라 모니터링한다 (도 5). 어세이의 게놈 플라스미드 버전과 마찬가지로, 시작 바이러스 스탁(stock)의 정확한 역가를 알 필요가 없다. 전형적으로, 희석 시리즈(dilution series)는 도 5에 개시된 바와 같이, 1:100 내지 1:218,700의 범위의 희석 시리즈와 같이 초기 감염을 위해 사용된다. 1:100의 희석은 일반적으로 모든 세포의 감염을 초래하지만, 1:218,700의 희석은 일반적으로 매우 적은 세포의 초기 감염을 유도한다. 이 실시예에서, 96-웰 플레이트를 사용하고, 11개의 상이한 바이러스 컨스트럭트를 8개의 상이한 희석액 (1:100, 1:300, 1:900, 1:2700, 1:8100, 1:24,300, 1:72,900 및 1:218,700)에서 동시에 시험한다. 플레이트는 또한 4개 웰의 거짓-감염된 세포 및 4개 웰의 FLUORESBRITETM 비드를 포함한다. 일단 세포가 감염되면, 플레이트를 지속적인 형광 모니터링을 위해 TECANTM 플레이트 리더기에 배치한다. 수집된 데이터는 각 컨스트럭트의 ln-슬로프를 계산하기 위해 사용된다 (도 8).
TECANTM 플레이트 리더기는 또한 (CO2 및 O2 수준뿐만 아니라 적절한 온도를 유지하는) 배양 기능을 제공한다. ln-슬로프를 계산하기 위해 15분마다 데이터 포인트를 취한다. 이러한 방법을 사용하여, 다양한 바이러스 사이 및 다른 세포 유형 사이의 동역학을 신속하고 효율적으로 비교할 수 있다. 예를 들어, 특정 재조합 아데노바이러스가 종양살상(oncolytic) 바이러스로서 치료용으로 사용될 수 있는지 여부를 평가하기 위해, 이 어세이는 종양 세포에서 높은 복제 동역학을 나타내지만, 비-종양 세포에서 트린 바이러스 동역학을 나타내는 바이러스를 발견하는데 이용될 수 있다. 또한, 재조합 바이러스의 바이러스 동역학은 이 어세이에서 관심 종양 세포 유형을 감염시키거나 형질감염시킴으로써 평가될 수 있다.
로그-슬로프(log-slope) 계산
로그-슬로프를 측정하기 위해, 형광 강도 대 시간의 선형 플롯을 각 시간 포인트에서 측정된 형광 강도의 자연 로그를 취하여 세미-로그 플롯으로 변환시킨다. 형광 강도는 바이러스 복제 동안 지수의 성장을 나타내기 때문에, ln (형광 강도) 대 시간을 플로팅할 때, 이 변환은 직선을 나타낸다. 그런 다음 이 직선을 표준 최소-제곱법을 사용하여 맞춘다. 이 피팅에 의해 생성된 결과 슬로프는 형광 대 시간의 ln-슬로프이고, 따라서 바이러스 성장 대 시간의 ln-슬로프이다. 방정식은 아래와 같다.
Figure 112021060760163-pat00003
; 여기서 FI는 측정된 형광 강도이고, t는 시간이며, F0은 시간 = t0일 때의 초기 형광 강도이고, α는 ln-슬로프이다.
양 변에 자연 대수를 취하라:
Figure 112021060760163-pat00004
우변은 이제 α의 ln-슬로프를 가진 직선 방정식이다.
본 개시의 원리에 대한 많은 가능한 실시양태가 적용될 수 있다는 관점에서, 도시된 실시양태는 오직 본 발명의 실시예일 뿐이고 본 발명의 범위를 제한하는 것으로 받아들여서는 안된다. 오히려, 본 발명의 범위는 하기 청구범위에 의해 정의된다. 따라서 우리는 이 청구범위의 범위 및 사상 내에서 나오는 모든 것을 우리의 발명으로 청구한다
<110> SALK INSTITUTE FOR BIOLOGICAL STUDIES <120> EXOGENOUS GENE EXPRESSION IN THERAPEUTIC ADENOVIRUS FOR MINIMAL IMPACT ON VIRAL KINETICS <130> 2021FPI-05-007/US <150> US 62/298,653 <151> 2016-02-23 <150> PCT/US 2017/019086 <151> 2017-02-23 <160> 23 <170> PatentIn version 3.5 <210> 1 <211> 36791 <212> DNA <213> Artificial Sequence <220> <223> Synthetic adenovirus genome YPet-P2A-E1A <400> 1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420 cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480 tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540 tccgacaccg ggactgaaaa tggtgagcaa aggcgaagag ctgttcaccg gcgtggtgcc 600 catcctggtg gagctggacg gcgacgtgaa cggccacaag ttcagcgtga gcggcgaggg 660 cgagggcgac gccacctacg gcaagctgac cctgaagctg ctgtgcacca ccggcaagct 720 gcccgtgccc tggcccaccc tggtgaccac cctgggctac ggcgtgcagt gcttcgcccg 780 gtaccccgac cacatgaagc agcacgactt cttcaagagc gccatgcccg agggctacgt 840 gcaggagcgg accatcttct tcaaggacga cggcaactac aagacccggg ccgaggtgaa 900 gttcgagggc gacaccctgg tgaaccggat cgagctgaag ggcatcgact tcaaggagga 960 cggcaacatc ctgggccaca agctggagta caactacaac agccacaacg tgtacatcac 1020 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccggcaca acatcgagga 1080 cggcggcgtg cagctggccg accactacca gcagaacacc cccatcggcg acggccccgt 1140 gctgctgccc gacaaccact acctgagcta ccagagcgcc ctgttcaagg accccaacga 1200 gaagcgggac cacatggtgc tgctggagtt cctgaccgcc gccggcatca ccgagggcat 1260 gaacgagctc tataagggaa gcggagctac taacttcagc ctgctgaagc aggctggtga 1320 cgtcgaggag aatcctggcc caatgagaca tattatctgc cacggaggtg ttattaccga 1380 agaaatggcc gccagtcttt tggaccagct gatcgaagag gtactggctg ataatcttcc 1440 acctcctagc cattttgaac cacctaccct tcacgaactg tatgatttag acgtgacggc 1500 ccccgaagat cccaacgagg aggcggtttc gcagattttt cccgactctg taatgttggc 1560 ggtgcaggaa gggattgact tactcacttt tccgccggcg cccggttctc cggagccgcc 1620 tcacctttcc cggcagcccg agcagccgga gcagagagcc ttgggtccgg tttctatgcc 1680 aaaccttgta ccggaggtga tcgatcttac ctgccacgag gctggctttc cacccagtga 1740 cgacgaggat gaagagggtg aggagtttgt gttagattat gtggagcacc ccgggcacgg 1800 ttgcaggtct tgtcattatc accggaggaa tacgggggac ccagatatta tgtgttcgct 1860 ttgctatatg aggacctgtg gcatgtttgt ctacagtaag tgaaaattat gggcagtggg 1920 tgatagagtg gtgggtttgg tgtggtaatt ttttttttaa tttttacagt tttgtggttt 1980 aaagaatttt gtattgtgat ttttttaaaa ggtcctgtgt ctgaacctga gcctgagccc 2040 gagccagaac cggagcctgc aagacctacc cgccgtccta aaatggcgcc tgctatcctg 2100 agacgcccga catcacctgt gtctagagaa tgcaatagta gtacggatag ctgtgactcc 2160 ggtccttcta acacacctcc tgagatacac ccggtggtcc cgctgtgccc cattaaacca 2220 gttgccgtga gagttggtgg gcgtcgccag gctgtggaat gtatcgagga cttgcttaac 2280 gagcctgggc aacctttgga cttgagctgt aaacgcccca ggccataagg tgtaaacctg 2340 tgattgcgtg tgtggttaac gcctttgttt gctgaatgag ttgatgtaag tttaataaag 2400 ggtgagataa tgtttaactt gcatggcgtg ttaaatgggg cggggcttaa agggtatata 2460 atgcgccgtg ggctaatctt ggttacatct gacctcatgg aggcttggga gtgtttggaa 2520 gatttttctg ctgtgcgtaa cttgctggaa cagagctcta acagtacctc ttggttttgg 2580 aggtttctgt ggggctcatc ccaggcaaag ttagtctgca gaattaagga ggattacaag 2640 tgggaatttg aagagctttt gaaatcctgt ggtgagctgt ttgattcttt gaatctgggt 2700 caccaggcgc ttttccaaga gaaggtcatc aagactttgg atttttccac accggggcgc 2760 gctgcggctg ctgttgcttt tttgagtttt ataaaggata aatggagcga agaaacccat 2820 ctgagcgggg ggtacctgct ggattttctg gccatgcatc tgtggagagc ggttgtgaga 2880 cacaagaatc gcctgctact gttgtcttcc gtccgcccgg cgataatacc gacggaggag 2940 cagcagcagc agcaggagga agccaggcgg cggcggcagg agcagagccc atggaacccg 3000 agagccggcc tggaccctcg ggaatgaatg ttgtacaggt ggctgaactg tatccagaac 3060 tgagacgcat tttgacaatt acagaggatg ggcaggggct aaagggggta aagagggagc 3120 ggggggcttg tgaggctaca gaggaggcta ggaatctagc ttttagctta atgaccagac 3180 accgtcctga gtgtattact tttcaacaga tcaaggataa ttgcgctaat gagcttgatc 3240 tgctggcgca gaagtattcc atagagcagc tgaccactta ctggctgcag ccaggggatg 3300 attttgagga ggctattagg gtatatgcaa aggtggcact taggccagat tgcaagtaca 3360 agatcagcaa acttgtaaat atcaggaatt gttgctacat ttctgggaac ggggccgagg 3420 tggagataga tacggaggat agggtggcct ttagatgtag catgataaat atgtggccgg 3480 gggtgcttgg catggacggg gtggttatta tgaatgtaag gtttactggc cccaatttta 3540 gcggtacggt tttcctggcc aataccaacc ttatcctaca cggtgtaagc ttctatgggt 3600 ttaacaatac ctgtgtggaa gcctggaccg atgtaagggt tcggggctgt gccttttact 3660 gctgctggaa gggggtggtg tgtcgcccca aaagcagggc ttcaattaag aaatgcctct 3720 ttgaaaggtg taccttgggt atcctgtctg agggtaactc cagggtgcgc cacaatgtgg 3780 cctccgactg tggttgcttc atgctagtga aaagcgtggc tgtgattaag cataacatgg 3840 tatgtggcaa ctgcgaggac agggcctctc agatgctgac ctgctcggac ggcaactgtc 3900 acctgctgaa gaccattcac gtagccagcc actctcgcaa ggcctggcca gtgtttgagc 3960 ataacatact gacccgctgt tccttgcatt tgggtaacag gaggggggtg ttcctacctt 4020 accaatgcaa tttgagtcac actaagatat tgcttgagcc cgagagcatg tccaaggtga 4080 acctgaacgg ggtgtttgac atgaccatga agatctggaa ggtgctgagg tacgatgaga 4140 cccgcaccag gtgcagaccc tgcgagtgtg gcggtaaaca tattaggaac cagcctgtga 4200 tgctggatgt gaccgaggag ctgaggcccg atcacttggt gctggcctgc acccgcgctg 4260 agtttggctc tagcgatgaa gatacagatt gaggtactga aatgtgtggg cgtggcttaa 4320 gggtgggaaa gaatatataa ggtgggggtc ttatgtagtt ttgtatctgt tttgcagcag 4380 ccgccgccgc ccaacttttc tatacaaagt tgtaatgagc accaactcgt ttgatggaag 4440 cattgtgagc tcatatttga caacgcgcat gcccccatgg gccggggtgc gtcagaatgt 4500 gatgggctcc agcattgatg gtcgccccgt cctgcccgca aactctacta ccttgaccta 4560 cgagaccgtg tctggaacgc cgttggagac tgcagcctcc gccgccgctt cagccgctgc 4620 agccaccgcc cgcgggattg tgactgactt tgctttcctg agcccgcttg caagcagtgc 4680 agcttcccgt tcatccgccc gcgatgacaa gttgacggct cttttggcac aattggattc 4740 tttgacccgg gaacttaatg tcgtttctca gcagctgttg gatctgcgcc agcaggtttc 4800 tgccctgaag gcttcctccc ctcccaatgc ggtttaaaac ataaataaaa aaccagactc 4860 tgtttggatt tggatcaagc ataagtgtct tgctgtcttt atttaggggt tttgcgcgcg 4920 cggtaggccc gggaccagcg gtctcggtcg ttgagggtcc tgtgtatttt ttccaggacg 4980 tggtaaaggt gactctggat gttcagatac atgggcataa gcccgtctct ggggtggagg 5040 tagcaccact gcagagcttc atgctgcggg gtggtgttgt agatgatcca gtcgtagcag 5100 gagcgctggg cgtggtgcct aaaaatgtct ttcagtagca agctgattgc caggggcagg 5160 cccttggtgt aagtgtttac aaagcggtta agctgggatg ggtgcatacg tggggatatg 5220 agatgcatct tggactgtat ttttaggttg gctatgttcc cagccatatc cctccgggga 5280 ttcatgttgt gcagaaccac cagcacagtg tatccggtgc acttgggaaa tttgtcatgt 5340 agcttagaag gaaatgcgtg gaagaacttg gagacgccct tgtgacctcc aagattttcc 5400 atgcattcgt ccataatgat ggcaatgggc ccacgggcgg cggcctgggc gaagatattt 5460 ctgggatcac taacgtcata gttgtgttcc aggatgagat cgtcataggc catttttaca 5520 aagcgcgggc ggagggtgcc agactgcggt ataatggttc catccggccc aggggcgtag 5580 ttaccctcac agatttgcat ttcccacgct ttgagttcag atggggggat catgtctacc 5640 tgcggggcga tgaagaaaac ggtttccggg gtaggggaga tcagctggga agaaagcagg 5700 ttcctgagca gctgcgactt accgcagccg gtgggcccgt aaatcacacc tattaccggg 5760 tgcaactggt agttaagaga gctgcagctg ccgtcatccc tgagcagggg ggccacttcg 5820 ttaagcatgt ccctgactcg catgttttcc ctgaccaaat ccgccagaag gcgctcgccg 5880 cccagcgata gcagttcttg caaggaagca aagtttttca acggtttgag accgtccgcc 5940 gtaggcatgc ttttgagcgt ttgaccaagc agttccaggc ggtcccacag ctcggtcacc 6000 tgctctacgg catctcgatc cagcatatct cctcgtttcg cgggttgggg cggctttcgc 6060 tgtacggcag tagtcggtgc tcgtccagac gggccagggt catgtctttc cacgggcgca 6120 gggtcctcgt cagcgtagtc tgggtcacgg tgaaggggtg cgctccgggc tgcgcgctgg 6180 ccagggtgcg cttgaggctg gtcctgctgg tgctgaagcg ctgccggtct tcgccctgcg 6240 cgtcggccag gtagcatttg accatggtgt catagtccag cccctccgcg gcgtggccct 6300 tggcgcgcag cttgcccttg gaggaggcgc cgcacgaggg gcagtgcaga cttttgaggg 6360 cgtagagctt gggcgcgaga aataccgatt ccggggagta ggcatccgcg ccgcaggccc 6420 cgcagacggt ctcgcattcc acgagccagg tgagctctgg ccgttcgggg tcaaaaacca 6480 ggtttccccc atgctttttg atgcgtttct tacctctggt ttccatgagc cggtgtccac 6540 gctcggtgac gaaaaggctg tccgtgtccc cgtatacaga cttgagaggc ctgtcctcga 6600 gcggtgttcc gcggtcctcc tcgtatagaa actcggacca ctctgagaca aaggctcgcg 6660 tccaggccag cacgaaggag gctaagtggg aggggtagcg gtcgttgtcc actagggggt 6720 ccactcgctc cagggtgtga agacacatgt cgccctcttc ggcatcaagg aaggtgattg 6780 gtttgtaggt gtaggccacg tgaccgggtg ttcctgaagg ggggctataa aagggggtgg 6840 gggcgcgttc gtcctcactc tcttccgcat cgctgtctgc gagggccagc tgttggggtg 6900 agtactccct ctgaaaagcg ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg 6960 aggaggattt gatattcacc tggcccgcgg tgatgccttt gagggtggcc gcatccatct 7020 ggtcagaaaa gacaatcttt ttgttgtcaa gcttggtggc aaacgacccg tagagggcgt 7080 tggacagcaa cttggcgatg gagcgcaggg tttggttttt gtcgcgatcg gcgcgctcct 7140 tggccgcgat gtttagctgc acgtattcgc gcgcaacgca ccgccattcg ggaaagacgg 7200 tggtgcgctc gtcgggcacc aggtgcacgc gccaaccgcg gttgtgcagg gtgacaaggt 7260 caacgctggt ggctacctct ccgcgtaggc gctcgttggt ccagcagagg cggccgccct 7320 tgcgcgagca gaatggcggt agggggtcta gctgcgtctc gtccgggggg tctgcgtcca 7380 cggtaaagac cccgggcagc aggcgcgcgt cgaagtagtc tatcttgcat ccttgcaagt 7440 ctagcgcctg ctgccatgcg cgggcggcaa gcgcgcgctc gtatgggttg agtgggggac 7500 cccatggcat ggggtgggtg agcgcggagg cgtacatgcc gcaaatgtcg taaacgtaga 7560 ggggctctct gagtattcca agatatgtag ggtagcatct tccaccgcgg atgctggcgc 7620 gcacgtaatc gtatagttcg tgcgagggag cgaggaggtc gggaccgagg ttgctacggg 7680 cgggctgctc tgctcggaag actatctgcc tgaagatggc atgtgagttg gatgatatgg 7740 ttggacgctg gaagacgttg aagctggcgt ctgtgagacc taccgcgtca cgcacgaagg 7800 aggcgtagga gtcgcgcagc ttgttgacca gctcggcggt gacctgcacg tctagggcgc 7860 agtagtccag ggtttccttg atgatgtcat acttatcctg tccctttttt ttccacagct 7920 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg 7980 cctccgaacg gtaagagcct agcatgtaga actggttgac ggcctggtag gcgcagcatc 8040 ccttttctac gggtagcgcg tatgcctgcg cggccttccg gagcgaggtg tgggtgagcg 8100 caaaggtgtc cctgaccatg actttgaggt actggtattt gaagtcagtg tcgtcgcatc 8160 cgccctgctc ccagagcaaa aagtccgtgc gctttttgga acgcggattt ggcagggcga 8220 aggtgacatc gttgaagagt atctttcccg cgcgaggcat aaagttgcgt gtgatgcgga 8280 agggtcccgg cacctcggaa cggttgttaa ttacctgggc ggcgagcacg atctcgtcaa 8340 agccgttgat gttgtggccc acaatgtaaa gttccaagaa gcgcgggatg cccttgatgg 8400 aaggcaattt tttaagttcc tcgtaggtga gctcttcagg ggagctgagc ccgtgctctg 8460 aaagggccca gtctgcaaga tgagggttgg aagcgacgaa tgagctccac aggtcacggg 8520 ccattagcat ttgcaggtgg tcgcgaaagg tcctaaactg gcgacctatg gccatttttt 8580 ctggggtgat gcagtagaag gtaagcgggt cttgttccca gcggtcccat ccaaggttcg 8640 cggctaggtc tcgcgcggca gtcactagag gctcatctcc gccgaacttc atgaccagca 8700 tgaagggcac gagctgcttc ccaaaggccc ccatccaagt ataggtctct acatcgtagg 8760 tgacaaagag acgctcggtg cgaggatgcg agccgatcgg gaagaactgg atctcccgcc 8820 accaattgga ggagtggcta ttgatgtggt gaaagtagaa gtccctgcga cgggccgaac 8880 actcgtgctg gcttttgtaa aaacgtgcgc agtactggca gcggtgcacg ggctgtacat 8940 cctgcacgag gttgacctga cgaccgcgca caaggaagca gagtgggaat ttgagcccct 9000 cgcctggcgg gtttggctgg tggtcttcta cttcggctgc ttgtccttga ccgtctggct 9060 gctcgagggg agttacggtg gatcggacca ccacgccgcg cgagcccaaa gtccagatgt 9120 ccgcgcgcgg cggtcggagc ttgatgacaa catcgcgcag atgggagctg tccatggtct 9180 ggagctcccg cggcgtcagg tcaggcggga gctcctgcag gtttacctcg catagacggg 9240 tcagggcgcg ggctagatcc aggtgatacc taatttccag gggctggttg gtggcggcgt 9300 cgatggcttg caagaggccg catccccgcg gcgcgactac ggtaccgcgc ggcgggcggt 9360 gggccgcggg ggtgtccttg gatgatgcat ctaaaagcgg tgacgcgggc gagcccccgg 9420 aggtaggggg ggctccggac ccgccgggag agggggcagg ggcacgtcgg cgccgcgcgc 9480 gggcaggagc tggtgctgcg cgcgtaggtt gctggcgaac gcgacgacgc ggcggttgat 9540 ctcctgaatc tggcgcctct gcgtgaagac gacgggcccg gtgagcttga gcctgaaaga 9600 gagttcgaca gaatcaattt cggtgtcgtt gacggcggcc tggcgcaaaa tctcctgcac 9660 gtctcctgag ttgtcttgat aggcgatctc ggccatgaac tgctcgatct cttcctcctg 9720 gagatctccg cgtccggctc gctccacggt ggcggcgagg tcgttggaaa tgcgggccat 9780 gagctgcgag aaggcgttga ggcctccctc gttccagacg cggctgtaga ccacgccccc 9840 ttcggcatcg cgggcgcgca tgaccacctg cgcgagattg agctccacgt gccgggcgaa 9900 gacggcgtag tttcgcaggc gctgaaagag gtagttgagg gtggtggcgg tgtgttctgc 9960 cacgaagaag tacataaccc agcgtcgcaa cgtggattcg ttgatatccc ccaaggcctc 10020 aaggcgctcc atggcctcgt agaagtccac ggcgaagttg aaaaactggg agttgcgcgc 10080 cgacacggtt aactcctcct ccagaagacg gatgagctcg gcgacagtgt cgcgcacctc 10140 gcgctcaaag gctacagggg cctcttcttc ttcttcaatc tcctcttcca taagggcctc 10200 cccttcttct tcttctggcg gcggtggggg aggggggaca cggcggcgac gacggcgcac 10260 cgggaggcgg tcgacaaagc gctcgatcat ctccccgcgg cgacggcgca tggtctcggt 10320 gacggcgcgg ccgttctcgc gggggcgcag ttggaagacg ccgcccgtca tgtcccggtt 10380 atgggttggc ggggggctgc catgcggcag ggatacggcg ctaacgatgc atctcaacaa 10440 ttgttgtgta ggtactccgc cgccgaggga cctgagcgag tccgcatcga ccggatcgga 10500 aaacctctcg agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc 10560 gggcggcagc gggcggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt 10620 aaagtaggcg gtcttgagac ggcggatggt cgacagaagc accatgtcct tgggtccggc 10680 ctgctgaatg cgcaggcggt cggccatgcc ccaggcttcg ttttgacatc ggcgcaggtc 10740 tttgtagtag tcttgcatga gcctttctac cggcacttct tcttctcctt cctcttgtcc 10800 tgcatctctt gcatctatcg ctgcggcggc ggcggagttt ggccgtaggt ggcgccctct 10860 tcctcccatg cgtgtgaccc cgaagcccct catcggctga agcagggcta ggtcggcgac 10920 aacgcgctcg gctaatatgg cctgctgcac ctgcgtgagg gtagactgga agtcatccat 10980 gtccacaaag cggtggtatg cgcccgtgtt gatggtgtaa gtgcagttgg ccataacgga 11040 ccagttaacg gtctggtgac ccggctgcga gagctcggtg tacctgagac gcgagtaagc 11100 cctcgagtca aatacgtagt cgttgcaagt ccgcaccagg tactggtatc ccaccaaaaa 11160 gtgcggcggc ggctggcggt agaggggcca gcgtagggtg gccggggctc cgggggcgag 11220 atcttccaac ataaggcgat gatatccgta gatgtacctg gacatccagg tgatgccggc 11280 ggcggtggtg gaggcgcgcg gaaagtcgcg gacgcggttc cagatgttgc gcagcggcaa 11340 aaagtgctcc atggtcggga cgctctggcc ggtcaggcgc gcgcaatcgt tgacgctcta 11400 gaccgtgcaa aaggagagcc tgtaagcggg cactcttccg tggtctggtg gataaattcg 11460 caagggtatc atggcggacg accggggttc gagccccgta tccggccgtc cgccgtgatc 11520 catgcggtta ccgcccgcgt gtcgaaccca ggtgtgcgac gtcagacaac gggggagtgc 11580 tccttttggc ttccttccag gcgcggcggc tgctgcgcta gcttttttgg ccactggccg 11640 cgcgcagcgt aagcggttag gctggaaagc gaaagcatta agtggctcgc tccctgtagc 11700 cggagggtta ttttccaagg gttgagtcgc gggacccccg gttcgagtct cggaccggcc 11760 ggactgcggc gaacgggggt ttgcctcccc gtcatgcaag accccgcttg caaattcctc 11820 cggaaacagg gacgagcccc ttttttgctt ttcccagatg catccggtgc tgcggcagat 11880 gcgcccccct cctcagcagc ggcaagagca agagcagcgg cagacatgca gggcaccctc 11940 ccctcctcct accgcgtcag gaggggcgac atccgcggtt gacgcggcag cagatggtga 12000 ttacgaaccc ccgcggcgcc gggcccggca ctacctggac ttggaggagg gcgagggcct 12060 ggcgcggcta ggagcgccct ctcctgagcg gtacccaagg gtgcagctga agcgtgatac 12120 gcgtgaggcg tacgtgccgc ggcagaacct gtttcgcgac cgcgagggag aggagcccga 12180 ggagatgcgg gatcgaaagt tccacgcagg gcgcgagctg cggcatggcc tgaatcgcga 12240 gcggttgctg cgcgaggagg actttgagcc cgacgcgcga accgggatta gtcccgcgcg 12300 cgcacacgtg gcggccgccg acctggtaac cgcatacgag cagacggtga accaggagat 12360 taactttcaa aaaagcttta acaaccacgt gcgtacgctt gtggcgcgcg aggaggtggc 12420 tataggactg atgcatctgt gggactttgt aagcgcgctg gagcaaaacc caaatagcaa 12480 gccgctcatg gcgcagctgt tccttatagt gcagcacagc agggacaacg aggcattcag 12540 ggatgcgctg ctaaacatag tagagcccga gggccgctgg ctgctcgatt tgataaacat 12600 cctgcagagc atagtggtgc aggagcgcag cttgagcctg gctgacaagg tggccgccat 12660 caactattcc atgcttagcc tgggcaagtt ttacgcccgc aagatatacc atacccctta 12720 cgttcccata gacaaggagg taaagatcga ggggttctac atgcgcatgg cgctgaaggt 12780 gcttaccttg agcgacgacc tgggcgttta tcgcaacgag cgcatccaca aggccgtgag 12840 cgtgagccgg cggcgcgagc tcagcgaccg cgagctgatg cacagcctgc aaagggccct 12900 ggctggcacg ggcagcggcg atagagaggc cgagtcctac tttgacgcgg gcgctgacct 12960 gcgctgggcc ccaagccgac gcgccctgga ggcagctggg gccggacctg ggctggcggt 13020 ggcacccgcg cgcgctggca acgtcggcgg cgtggaggaa tatgacgagg acgatgagta 13080 cgagccagag gacggcgagt actaagcggt gatgtttctg atcagatgat gcaagacgca 13140 acggacccgg cggtgcgggc ggcgctgcag agccagccgt ccggccttaa ctccacggac 13200 gactggcgcc aggtcatgga ccgcatcatg tcgctgactg cgcgcaatcc tgacgcgttc 13260 cggcagcagc cgcaggccaa ccggctctcc gcaattctgg aagcggtggt cccggcgcgc 13320 gcaaacccca cgcacgagaa ggtgctggcg atcgtaaacg cgctggccga aaacagggcc 13380 atccggcccg acgaggccgg cctggtctac gacgcgctgc ttcagcgcgt ggctcgttac 13440 aacagcggca acgtgcagac caacctggac cggctggtgg gggatgtgcg cgaggccgtg 13500 gcgcagcgtg agcgcgcgca gcagcagggc aacctgggct ccatggttgc actaaacgcc 13560 ttcctgagta cacagcccgc caacgtgccg cggggacagg aggactacac caactttgtg 13620 agcgcactgc ggctaatggt gactgagaca ccgcaaagtg aggtgtacca gtctgggcca 13680 gactattttt tccagaccag tagacaaggc ctgcagaccg taaacctgag ccaggctttc 13740 aaaaacttgc aggggctgtg gggggtgcgg gctcccacag gcgaccgcgc gaccgtgtct 13800 agcttgctga cgcccaactc gcgcctgttg ctgctgctaa tagcgccctt cacggacagt 13860 ggcagcgtgt cccgggacac atacctaggt cacttgctga cactgtaccg cgaggccata 13920 ggtcaggcgc atgtggacga gcatactttc caggagatta caagtgtcag ccgcgcgctg 13980 gggcaggagg acacgggcag cctggaggca accctaaact acctgctgac caaccggcgg 14040 cagaagatcc cctcgttgca cagtttaaac agcgaggagg agcgcatttt gcgctacgtg 14100 cagcagagcg tgagccttaa cctgatgcgc gacggggtaa cgcccagcgt ggcgctggac 14160 atgaccgcgc gcaacatgga accgggcatg tatgcctcaa accggccgtt tatcaaccgc 14220 ctaatggact acttgcatcg cgcggccgcc gtgaaccccg agtatttcac caatgccatc 14280 ttgaacccgc actggctacc gccccctggt ttctacaccg ggggattcga ggtgcccgag 14340 ggtaacgatg gattcctctg ggacgacata gacgacagcg tgttttcccc gcaaccgcag 14400 accctgctag agttgcaaca gcgcgagcag gcagaggcgg cgctgcgaaa ggaaagcttc 14460 cgcaggccaa gcagcttgtc cgatctaggc gctgcggccc cgcggtcaga tgctagtagc 14520 ccatttccaa gcttgatagg gtctcttacc agcactcgca ccacccgccc gcgcctgctg 14580 ggcgaggagg agtacctaaa caactcgctg ctgcagccgc agcgcgaaaa aaacctgcct 14640 ccggcatttc ccaacaacgg gatagagagc ctagtggaca agatgagtag atggaagacg 14700 tacgcgcagg agcacaggga cgtgccaggc ccgcgcccgc ccacccgtcg tcaaaggcac 14760 gaccgtcagc ggggtctggt gtgggaggac gatgactcgg cagacgacag cagcgtcctg 14820 gatttgggag ggagtggcaa cccgtttgcg caccttcgcc ccaggctggg gagaatgttt 14880 taaaaaaaaa aaagcatgat gcaaaataaa aaactcacca aggccatggc accgagcgtt 14940 ggttttcttg tattcccctt agtatgcggc gcgcggcgat gtatgaggaa ggtcctcctc 15000 cctcctacga gagtgtggtg agcgcggcgc cagtggcggc ggcgctgggt tctcccttcg 15060 atgctcccct ggacccgccg tttgtgcctc cgcggtacct gcggcctacc ggggggagaa 15120 acagcatccg ttactctgag ttggcacccc tattcgacac cacccgtgtg tacctggtgg 15180 acaacaagtc aacggatgtg gcatccctga actaccagaa cgaccacagc aactttctga 15240 ccacggtcat tcaaaacaat gactacagcc cgggggaggc aagcacacag accatcaatc 15300 ttgacgaccg gtcgcactgg ggcggcgacc tgaaaaccat cctgcatacc aacatgccaa 15360 atgtgaacga gttcatgttt accaataagt ttaaggcgcg ggtgatggtg tcgcgcttgc 15420 ctactaagga caatcaggtg gagctgaaat acgagtgggt ggagttcacg ctgcccgagg 15480 gcaactactc cgagaccatg accatagacc ttatgaacaa cgcgatcgtg gagcactact 15540 tgaaagtggg cagacagaac ggggttctgg aaagcgacat cggggtaaag tttgacaccc 15600 gcaacttcag actggggttt gaccccgtca ctggtcttgt catgcctggg gtatatacaa 15660 acgaagcctt ccatccagac atcattttgc tgccaggatg cggggtggac ttcacccaca 15720 gccgcctgag caacttgttg ggcatccgca agcggcaacc cttccaggag ggctttagga 15780 tcacctacga tgatctggag ggtggtaaca ttcccgcact gttggatgtg gacgcctacc 15840 aggcgagctt gaaagatgac accgaacagg gcgggggtgg cgcaggcggc agcaacagca 15900 gtggcagcgg cgcggaagag aactccaacg cggcagccgc ggcaatgcag ccggtggagg 15960 acatgaacga tcatgccatt cgcggcgaca cctttgccac acgggctgag gagaagcgcg 16020 ctgaggccga agcagcggcc gaagctgccg cccccgctgc gcaacccgag gtcgagaagc 16080 ctcagaagaa accggtgatc aaacccctga cagaggacag caagaaacgc agttacaacc 16140 taataagcaa tgacagcacc ttcacccagt accgcagctg gtaccttgca tacaactacg 16200 gcgaccctca gaccggaatc cgctcatgga ccctgctttg cactcctgac gtaacctgcg 16260 gctcggagca ggtctactgg tcgttgccag acatgatgca agaccccgtg accttccgct 16320 ccacgcgcca gatcagcaac tttccggtgg tgggcgccga gctgttgccc gtgcactcca 16380 agagcttcta caacgaccag gccgtctact cccaactcat ccgccagttt acctctctga 16440 cccacgtgtt caatcgcttt cccgagaacc agattttggc gcgcccgcca gcccccacca 16500 tcaccaccgt cagtgaaaac gttcctgctc tcacagatca cgggacgcta ccgctgcgca 16560 acagcatcgg aggagtccag cgagtgacca ttactgacgc cagacgccgc acctgcccct 16620 acgtttacaa ggccctgggc atagtctcgc cgcgcgtcct atcgagccgc actttttgag 16680 caagcatgtc catccttata tcgcccagca ataacacagg ctggggcctg cgcttcccaa 16740 gcaagatgtt tggcggggcc aagaagcgct ccgaccaaca cccagtgcgc gtgcgcgggc 16800 actaccgcgc gccctggggc gcgcacaaac gcggccgcac tgggcgcacc accgtcgatg 16860 acgccatcga cgcggtggtg gaggaggcgc gcaactacac gcccacgccg ccaccagtgt 16920 ccacagtgga cgcggccatt cagaccgtgg tgcgcggagc ccggcgctat gctaaaatga 16980 agagacggcg gaggcgcgta gcacgtcgcc accgccgccg acccggcact gccgcccaac 17040 gcgcggcggc ggccctgctt aaccgcgcac gtcgcaccgg ccgacgggcg gccatgcggg 17100 ccgctcgaag gctggccgcg ggtattgtca ctgtgccccc caggtccagg cgacgagcgg 17160 ccgccgcagc agccgcggcc attagtgcta tgactcaggg tcgcaggggc aacgtgtatt 17220 gggtgcgcga ctcggttagc ggcctgcgcg tgcccgtgcg cacccgcccc ccgcgcaact 17280 agattgcaag aaaaaactac ttagactcgt actgttgtat gtatccagcg gcggcggcgc 17340 gcaacgaagc tatgtccaag cgcaaaatca aagaagagat gctccaggtc atcgcgccgg 17400 agatctatgg ccccccgaag aaggaagagc aggattacaa gccccgaaag ctaaagcggg 17460 tcaaaaagaa aaagaaagat gatgatgatg aacttgacga cgaggtggaa ctgctgcacg 17520 ctaccgcgcc caggcgacgg gtacagtgga aaggtcgacg cgtaaaacgt gttttgcgac 17580 ccggcaccac cgtagtcttt acgcccggtg agcgctccac ccgcacctac aagcgcgtgt 17640 atgatgaggt gtacggcgac gaggacctgc ttgagcaggc caacgagcgc ctcggggagt 17700 ttgcctacgg aaagcggcat aaggacatgc tggcgttgcc gctggacgag ggcaacccaa 17760 cacctagcct aaagcccgta acactgcagc aggtgctgcc cgcgcttgca ccgtccgaag 17820 aaaagcgcgg cctaaagcgc gagtctggtg acttggcacc caccgtgcag ctgatggtac 17880 ccaagcgcca gcgactggaa gatgtcttgg aaaaaatgac cgtggaacct gggctggagc 17940 ccgaggtccg cgtgcggcca atcaagcagg tggcgccggg actgggcgtg cagaccgtgg 18000 acgttcagat acccactacc agtagcacca gtattgccac cgccacagag ggcatggaga 18060 cacaaacgtc cccggttgcc tcagcggtgg cggatgccgc ggtgcaggcg gtcgctgcgg 18120 ccgcgtccaa gacctctacg gaggtgcaaa cggacccgtg gatgtttcgc gtttcagccc 18180 cccggcgccc gcgcggttcg aggaagtacg gcgccgccag cgcgctactg cccgaatatg 18240 ccctacatcc ttccattgcg cctacccccg gctatcgtgg ctacacctac cgccccagaa 18300 gacgagcaac tacccgacgc cgaaccacca ctggaacccg ccgccgccgt cgccgtcgcc 18360 agcccgtgct ggccccgatt tccgtgcgca gggtggctcg cgaaggaggc aggaccctgg 18420 tgctgccaac agcgcgctac caccccagca tcgtttaaaa gccggtcttt gtggttcttg 18480 cagatatggc cctcacctgc cgcctccgtt tcccggtgcc gggattccga ggaagaatgc 18540 accgtaggag gggcatggcc ggccacggcc tgacgggcgg catgcgtcgt gcgcaccacc 18600 ggcggcggcg cgcgtcgcac cgtcgcatgc gcggcggtat cctgcccctc cttattccac 18660 tgatcgccgc ggcgattggc gccgtgcccg gaattgcatc cgtggccttg caggcgcaga 18720 gacactgatt aaaaacaagt tgcatgtgga aaaatcaaaa taaaaagtct ggactctcac 18780 gctcgcttgg tcctgtaact attttgtaga atggaagaca tcaactttgc gtctctggcc 18840 ccgcgacacg gctcgcgccc gttcatggga aactggcaag atatcggcac cagcaatatg 18900 agcggtggcg ccttcagctg gggctcgctg tggagcggca ttaaaaattt cggttccacc 18960 gttaagaact atggcagcaa ggcctggaac agcagcacag gccagatgct gagggataag 19020 ttgaaagagc aaaatttcca acaaaaggtg gtagatggcc tggcctctgg cattagcggg 19080 gtggtggacc tggccaacca ggcagtgcaa aataagatta acagtaagct tgatccccgc 19140 cctcccgtag aggagcctcc accggccgtg gagacagtgt ctccagaggg gcgtggcgaa 19200 aagcgtccgc gccccgacag ggaagaaact ctggtgacgc aaatagacga gcctccctcg 19260 tacgaggagg cactaaagca aggcctgccc accacccgtc ccatcgcgcc catggctacc 19320 ggagtgctgg gccagcacac acccgtaacg ctggacctgc ctccccccgc cgacacccag 19380 cagaaacctg tgctgccagg cccgaccgcc gttgttgtaa cccgtcctag ccgcgcgtcc 19440 ctgcgccgcg ccgccagcgg tccgcgatcg ttgcggcccg tagccagtgg caactggcaa 19500 agcacactga acagcatcgt gggtctgggg gtgcaatccc tgaagcgccg acgatgcttc 19560 tgaatagcta acgtgtcgta tgtgtgtcat gtatgcgtcc atgtcgccgc cagaggagct 19620 gctgagccgc cgcgcgcccg ctttccaaga tggctacccc ttcgatgatg ccgcagtggt 19680 cttacatgca catctcgggc caggacgcct cggagtacct gagccccggg ctggtgcagt 19740 ttgcccgcgc caccgagacg tacttcagcc tgaataacaa gtttagaaac cccacggtgg 19800 cgcctacgca cgacgtgacc acagaccggt cccagcgttt gacgctgcgg ttcatccctg 19860 tggaccgtga ggatactgcg tactcgtaca aggcgcggtt caccctagct gtgggtgata 19920 accgtgtgct ggacatggct tccacgtact ttgacatccg cggcgtgctg gacaggggcc 19980 ctacttttaa gccctactct ggcactgcct acaacgccct ggctcccaag ggtgccccaa 20040 atccttgcga atgggatgaa gctgctactg ctcttgaaat aaacctagaa gaagaggacg 20100 atgacaacga agacgaagta gacgagcaag ctgagcagca aaaaactcac gtatttgggc 20160 aggcgcctta ttctggtata aatattacaa aggagggtat tcaaataggt gtcgaaggtc 20220 aaacacctaa atatgccgat aaaacatttc aacctgaacc tcaaatagga gaatctcagt 20280 ggtacgaaac tgaaattaat catgcagctg ggagagtcct taaaaagact accccaatga 20340 aaccatgtta cggttcatat gcaaaaccca caaatgaaaa tggagggcaa ggcattcttg 20400 taaagcaaca aaatggaaag ctagaaagtc aagtggaaat gcaatttttc tcaactactg 20460 aggcgaccgc aggcaatggt gataacttga ctcctaaagt ggtattgtac agtgaagatg 20520 tagatataga aaccccagac actcatattt cttacatgcc cactattaag gaaggtaact 20580 cacgagaact aatgggccaa caatctatgc ccaacaggcc taattacatt gcttttaggg 20640 acaattttat tggtctaatg tattacaaca gcacgggtaa tatgggtgtt ctggcgggcc 20700 aagcatcgca gttgaatgct gttgtagatt tgcaagacag aaacacagag ctttcatacc 20760 agcttttgct tgattccatt ggtgatagaa ccaggtactt ttctatgtgg aatcaggctg 20820 ttgacagcta tgatccagat gttagaatta ttgaaaatca tggaactgaa gatgaacttc 20880 caaattactg ctttccactg ggaggtgtga ttaatacaga gactcttacc aaggtaaaac 20940 ctaaaacagg tcaggaaaat ggatgggaaa aagatgctac agaattttca gataaaaatg 21000 aaataagagt tggaaataat tttgccatgg aaatcaatct aaatgccaac ctgtggagaa 21060 atttcctgta ctccaacata gcgctgtatt tgcccgacaa gctaaagtac agtccttcca 21120 acgtaaaaat ttctgataac ccaaacacct acgactacat gaacaagcga gtggtggctc 21180 ccgggttagt ggactgctac attaaccttg gagcacgctg gtcccttgac tatatggaca 21240 acgtcaaccc atttaaccac caccgcaatg ctggcctgcg ctaccgctca atgttgctgg 21300 gcaatggtcg ctatgtgccc ttccacatcc aggtgcctca gaagttcttt gccattaaaa 21360 acctccttct cctgccgggc tcatacacct acgagtggaa cttcaggaag gatgttaaca 21420 tggttctgca gagctcccta ggaaatgacc taagggttga cggagccagc attaagtttg 21480 atagcatttg cctttacgcc accttcttcc ccatggccca caacaccgcc tccacgcttg 21540 aggccatgct tagaaacgac accaacgacc agtcctttaa cgactatctc tccgccgcca 21600 acatgctcta ccctataccc gccaacgcta ccaacgtgcc catatccatc ccctcccgca 21660 actgggcggc tttccgcggc tgggccttca cgcgccttaa gactaaggaa accccatcac 21720 tgggctcggg ctacgaccct tattacacct actctggctc tataccctac ctagatggaa 21780 ccttttacct caaccacacc tttaagaagg tggccattac ctttgactct tctgtcagct 21840 ggcctggcaa tgaccgcctg cttaccccca acgagtttga aattaagcgc tcagttgacg 21900 gggagggtta caacgttgcc cagtgtaaca tgaccaaaga ctggttcctg gtacaaatgc 21960 tagctaacta caacattggc taccagggct tctatatccc agagagctac aaggaccgca 22020 tgtactcctt ctttagaaac ttccagccca tgagccgtca ggtggtggat gatactaaat 22080 acaaggacta ccaacaggtg ggcatcctac accaacacaa caactctgga tttgttggct 22140 accttgcccc caccatgcgc gaaggacagg cctaccctgc taacttcccc tatccgctta 22200 taggcaagac cgcagttgac agcattaccc agaaaaagtt tctttgcgat cgcacccttt 22260 ggcgcatccc attctccagt aactttatgt ccatgggcgc actcacagac ctgggccaaa 22320 accttctcta cgccaactcc gcccacgcgc tagacatgac ttttgaggtg gatcccatgg 22380 acgagcccac ccttctttat gttttgtttg aagtctttga cgtggtccgt gtgcaccggc 22440 cgcaccgcgg cgtcatcgaa accgtgtacc tgcgcacgcc cttctcggcc ggcaacgcca 22500 caacataaag aagcaagcaa catcaacaac agctgccgcc atgggctcca gtgagcagga 22560 actgaaagcc attgtcaaag atcttggttg tgggccatat tttttgggca cctatgacaa 22620 gcgctttcca ggctttgttt ctccacacaa gctcgcctgc gccatagtca atacggccgg 22680 tcgcgagact gggggcgtac actggatggc ctttgcctgg aacccgcact caaaaacatg 22740 ctacctcttt gagccctttg gcttttctga ccagcgactc aagcaggttt accagtttga 22800 gtacgagtca ctcctgcgcc gtagcgccat tgcttcttcc cccgaccgct gtataacgct 22860 ggaaaagtcc acccaaagcg tacaggggcc caactcggcc gcctgtggac tattctgctg 22920 catgtttctc cacgcctttg ccaactggcc ccaaactccc atggatcaca accccaccat 22980 gaaccttatt accggggtac ccaactccat gctcaacagt ccccaggtac agcccaccct 23040 gcgtcgcaac caggaacagc tctacagctt cctggagcgc cactcgccct acttccgcag 23100 ccacagtgcg cagattagga gcgccacttc tttttgtcac ttgaaaaaca tgtaaaaata 23160 atgtactaga gacactttca ataaaggcaa atgcttttat ttgtacactc tcgggtgatt 23220 atttaccccc acccttgccg tctgcgccgt ttaaaaatca aaggggttct gccgcgcatc 23280 gctatgcgcc actggcaggg acacgttgcg atactggtgt ttagtgctcc acttaaactc 23340 aggcacaacc atccgcggca gctcggtgaa gttttcactc cacaggctgc gcaccatcac 23400 caacgcgttt agcaggtcgg gcgccgatat cttgaagtcg cagttggggc ctccgccctg 23460 cgcgcgcgag ttgcgataca cagggttgca gcactggaac actatcagcg ccgggtggtg 23520 cacgctggcc agcacgctct tgtcggagat cagatccgcg tccaggtcct ccgcgttgct 23580 cagggcgaac ggagtcaact ttggtagctg ccttcccaaa aagggcgcgt gcccaggctt 23640 tgagttgcac tcgcaccgta gtggcatcaa aaggtgaccg tgcccggtct gggcgttagg 23700 atacagcgcc tgcataaaag ccttgatctg cttaaaagcc acctgagcct ttgcgccttc 23760 agagaagaac atgccgcaag acttgccgga aaactgattg gccggacagg ccgcgtcgtg 23820 cacgcagcac cttgcgtcgg tgttggagat ctgcaccaca tttcggcccc accggttctt 23880 cacgatcttg gccttgctag actgctcctt cagcgcgcgc tgcccgtttt cgctcgtcac 23940 atccatttca atcacgtgct ccttatttat cataatgctt ccgtgtagac acttaagctc 24000 gccttcgatc tcagcgcagc ggtgcagcca caacgcgcag cccgtgggct cgtgatgctt 24060 gtaggtcacc tctgcaaacg actgcaggta cgcctgcagg aatcgcccca tcatcgtcac 24120 aaaggtcttg ttgctggtga aggtcagctg caacccgcgg tgctcctcgt tcagccaggt 24180 cttgcatacg gccgccagag cttccacttg gtcaggcagt agtttgaagt tcgcctttag 24240 atcgttatcc acgtggtact tgtccatcag cgcgcgcgca gcctccatgc ccttctccca 24300 cgcagacacg atcggcacac tcagcgggtt catcaccgta atttcacttt ccgcttcgct 24360 gggctcttcc tcttcctctt gcgtccgcat accacgcgcc actgggtcgt cttcattcag 24420 ccgccgcact gtgcgcttac ctcctttgcc atgcttgatt agcaccggtg ggttgctgaa 24480 acccaccatt tgtagcgcca catcttctct ttcttcctcg ctgtccacga ttacctctgg 24540 tgatggcggg cgctcgggct tgggagaagg gcgcttcttt ttcttcttgg gcgcaatggc 24600 caaatccgcc gccgaggtcg atggccgcgg gctgggtgtg cgcggcacca gcgcgtcttg 24660 tgatgagtct tcctcgtcct cggactcgat acgccgcctc atccgctttt ttgggggcgc 24720 ccggggaggc ggcggcgacg gggacgggga cgacacgtcc tccatggttg ggggacgtcg 24780 cgccgcaccg cgtccgcgct cgggggtggt ttcgcgctgc tcctcttccc gactggccat 24840 ttccttctcc tataggcaga aaaagatcat ggagtcagtc gagaagaagg acagcctaac 24900 cgccccctct gagttcgcca ccaccgcctc caccgatgcc gccaacgcgc ctaccacctt 24960 ccccgtcgag gcacccccgc ttgaggagga ggaagtgatt atcgagcagg acccaggttt 25020 tgtaagcgaa gacgacgagg accgctcagt accaacagag gataaaaagc aagaccagga 25080 caacgcagag gcaaacgagg aacaagtcgg gcggggggac gaaaggcatg gcgactacct 25140 agatgtggga gacgacgtgc tgttgaagca tctgcagcgc cagtgcgcca ttatctgcga 25200 cgcgttgcaa gagcgcagcg atgtgcccct cgccatagcg gatgtcagcc ttgcctacga 25260 acgccaccta ttctcaccgc gcgtaccccc caaacgccaa gaaaacggca catgcgagcc 25320 caacccgcgc ctcaacttct accccgtatt tgccgtgcca gaggtgcttg ccacctatca 25380 catctttttc caaaactgca agatacccct atcctgccgt gccaaccgca gccgagcgga 25440 caagcagctg gccttgcggc agggcgctgt catacctgat atcgcctcgc tcaacgaagt 25500 gccaaaaatc tttgagggtc ttggacgcga cgagaagcgc gcggcaaacg ctctgcaaca 25560 ggaaaacagc gaaaatgaaa gtcactctgg agtgttggtg gaactcgagg gtgacaacgc 25620 gcgcctagcc gtactaaaac gcagcatcga ggtcacccac tttgcctacc cggcacttaa 25680 cctacccccc aaggtcatga gcacagtcat gagtgagctg atcgtgcgcc gtgcgcagcc 25740 cctggagagg gatgcaaatt tgcaagaaca aacagaggag ggcctacccg cagttggcga 25800 cgagcagcta gcgcgctggc ttcaaacgcg cgagcctgcc gacttggagg agcgacgcaa 25860 actaatgatg gccgcagtgc tcgttaccgt ggagcttgag tgcatgcagc ggttctttgc 25920 tgacccggag atgcagcgca agctagagga aacattgcac tacacctttc gacagggcta 25980 cgtacgccag gcctgcaaga tctccaacgt ggagctctgc aacctggtct cctaccttgg 26040 aattttgcac gaaaaccgcc ttgggcaaaa cgtgcttcat tccacgctca agggcgaggc 26100 gcgccgcgac tacgtccgcg actgcgttta cttatttcta tgctacacct ggcagacggc 26160 catgggcgtt tggcagcagt gcttggagga gtgcaacctc aaggagctgc agaaactgct 26220 aaagcaaaac ttgaaggacc tatggacggc cttcaacgag cgctccgtgg ccgcgcacct 26280 ggcggacatc attttccccg aacgcctgct taaaaccctg caacagggtc tgccagactt 26340 caccagtcaa agcatgttgc agaactttag gaactttatc ctagagcgct caggaatctt 26400 gcccgccacc tgctgtgcac ttcctagcga ctttgtgccc attaagtacc gcgaatgccc 26460 tccgccgctt tggggccact gctaccttct gcagctagcc aactaccttg cctaccactc 26520 tgacataatg gaagacgtga gcggtgacgg tctactggag tgtcactgtc gctgcaacct 26580 atgcaccccg caccgctccc tggtttgcaa ttcgcagctg cttaacgaaa gtcaaattat 26640 cggtaccttt gagctgcagg gtccctcgcc tgacgaaaag tccgcggctc cggggttgaa 26700 actcactccg gggctgtgga cgtcggctta ccttcgcaaa tttgtacctg aggactacca 26760 cgcccacgag attaggttct acgaagacca atcccgcccg ccaaatgcgg agcttaccgc 26820 ctgcgtcatt acccagggcc acattcttgg ccaattgcaa gccatcaaca aagcccgcca 26880 agagtttctg ctacgaaagg gacggggggt ttacttggac ccccagtccg gcgaggagct 26940 caacccaatc cccccgccgc cgcagcccta tcagcagcag ccgcgggccc ttgcttccca 27000 ggatggcacc caaaaagaag ctgcagctgc cgccgccacc cacggacgag gaggaatact 27060 gggacagtca ggcagaggag gttttggacg aggaggagga ggacatgatg gaagactggg 27120 agagcctaga cgaggaagct tccgaggtcg aagaggtgtc agacgaaaca ccgtcaccct 27180 cggtcgcatt cccctcgccg gcgccccaga aatcggcaac cggttccagc atggctacaa 27240 cctccgctcc tcaggcgccg ccggcactgc ccgttcgccg acccaaccgt agatgggaca 27300 ccactggaac cagggccggt aagtccaagc agccgccgcc gttagcccaa gagcaacaac 27360 agcgccaagg ctaccgctca tggcgcgggc acaagaacgc catagttgct tgcttgcaag 27420 actgtggggg caacatctcc ttcgcccgcc gctttcttct ctaccatcac ggcgtggcct 27480 tcccccgtaa catcctgcat tactaccgtc atctctacag cccatactgc accggcggca 27540 gcggcagcgg cagcaacagc agcggccaca cagaagcaaa ggcgaccgga tagcaagact 27600 ctgacaaagc ccaagaaatc cacagcggcg gcagcagcag gaggaggagc gctgcgtctg 27660 gcgcccaacg aacccgtatc gacccgcgag cttagaaaca ggatttttcc cactctgtat 27720 gctatatttc aacagagcag gggccaagaa caagagctga aaataaaaaa caggtctctg 27780 cgatccctca cccgcagctg cctgtatcac aaaagcgaag atcagcttcg gcgcacgctg 27840 gaagacgcgg aggctctctt cagtaaatac tgcgcgctga ctcttaagga ctagtttcgc 27900 gccctttctc aaatttaagc gcgaaaacta cgtcatctcc agcggccaca cccggcgcca 27960 gcacctgtcg tcagcgccat ttcaactttg tatacaaaag ttgtgatgag caaggaaatt 28020 cccacgccct acatgtggag ttaccagcca caaatgggac ttgcggctgg agctgcccaa 28080 gactactcaa cccgaataaa ctacatgagc gcgggacccc acatgatatc ccgggtcaac 28140 ggaatacgcg cccaccgaaa ccgaattctc ctggaacagg cggctattac caccacacct 28200 cgtaataacc ttaatccccg tagttggccc gctgccctgg tgtaccagga aagtcccgct 28260 cccaccactg tggtacttcc cagagacgcc caggccgaag ttcagatgac taactcaggg 28320 gcgcagcttg cgggcggctt tcgtcacagg gtgcggtcgc ccgggcaggg tataactcac 28380 ctgacaatca gagggcgagg tattcagctc aacgacgagt cggtgagctc ctcgcttggt 28440 ctccgtccgg acgggacatt tcagatcggc ggcgccggcc gctcttcatt cacgcctcgt 28500 caggcaatcc taactctgca gacctcgtcc tctgagccgc gctctggagg cattggaact 28560 ctgcaattta ttgaggagtt tgtgccatcg gtctacttta accccttctc gggacctccc 28620 ggccactatc cggatcaatt tattcctaac tttgacgcgg taaaggactc ggcggacggc 28680 tacgactgaa tgttaagtgg agaggcagag caactgcgcc tgaaacacct ggtccactgt 28740 cgccgccaca agtgctttgc ccgcgactcc ggtgagtttt gctactttga attgcccgag 28800 gatcatatcg agggcccggc gcacggcgtc cggcttaccg cccagggaga gcttgcccgt 28860 agcctgattc gggagtttac ccagcgcccc ctgctagttg agcgggacag gggaccctgt 28920 gttctcactg tgatttgcaa ctgtcctaac cctggattac atcaagatct ttgttgccat 28980 ctctgtgctg agtataataa atacagaaat taaaatatac tggggctcct atcgccatcc 29040 tgtaaacgcc accgtcttca cccgcccaag caaaccaagg cgaaccttac ctggtacttt 29100 taacatctct ccctctgtga tttacaacag tttcaaccca gacggagtga gtctacgaga 29160 gaacctctcc gagctcagct actccatcag aaaaaacacc accctcctta cctgccggga 29220 acgtacgagt gcgtcaccgg ccgctgcacc acacctaccg cctgaccgta aaccagactt 29280 tttccggaca gacctcaata actctgttta ccagaacagg aggtgagctt agaaaaccct 29340 tagggtatta ggccaaaggc gcagctactg tggggtttat gaacaattca agcaactcta 29400 cgggctattc taattcaggt ttctctagaa tcggggttgg ggttattctc tgtcttgtga 29460 ttctctttat tcttatacta acgcttctct gcctaaggct cgccgcctgc tgtgtgcaca 29520 tttgcattta ttgtcagctt tttaaacgct ggggtcgcca cccaagatga ttaggtacat 29580 aatcctaggt ttactcaccc ttgcgtcagc ccacggtacc acccaaaagg tggattttaa 29640 ggagccagcc tgtaatgtta cattcgcagc tgaagctaat gagtgcacca ctcttataaa 29700 atgcaccaca gaacatgaaa agctgcttat tcgccacaaa aacaaaattg gcaagtatgc 29760 tgtttatgct atttggcagc caggtgacac tacagagtat aatgttacag ttttccaggg 29820 taaaagtcat aaaactttta tgtatacttt tccattttat gaaatgtgcg acattaccat 29880 gtacatgagc aaacagtata agttgtggcc cccacaaaat tgtgtggaaa acactggcac 29940 tttctgctgc actgctatgc taattacagt gctcgctttg gtctgtaccc tactctatat 30000 taaatacaaa agcagacgca gctttattga ggaaaagaaa atgccttaat ttactaagtt 30060 acaaagctaa tgtcaccact aactgcttta ctcgctgctt gcaaaacaaa ttcaaaaagt 30120 tagcattata attagaatag gatttaaacc ccccggtcat ttcctgctca ataccattcc 30180 cctgaacaat tgactctatg tgggatatgc tccagcgcta caaccttgaa gtcaggcttc 30240 ctggatgtca gcatctgact ttggccagca cctgtcccgc ggatttgttc cagtccaact 30300 acagcgaccc accctaacag agatgaccaa cacaaccaac gcggccgccg ctaccggact 30360 tacatctacc acaaatacac cccaagtttc tgcctttgtc aataactggg ataacttggg 30420 catgtggtgg ttctccatag cgcttatgtt tgtatgcctt attattatgt ggctcatctg 30480 ctgcctaaag cgcaaacgcg cccgaccacc catctatagt cccatcattg tgctacaccc 30540 aaacaatgat ggaatccata gattggacgg actgaaacac atgttctttt ctcttacagt 30600 atgattaaat gagacatgat tcctcgagtt tttatattac tgacccttgt tgcgcttttt 30660 tgtgcgtgct ccacattggc tgcggtttct cacatcgaag tagactgcat tccagccttc 30720 acagtctatt tgctttacgg atttgtcacc ctcacgctca tctgcagcct catcactgtg 30780 gtcatcgcct ttatccagtg cattgactgg gtctgtgtgc gctttgcata tctcagacac 30840 catccccagt acagggacag gactatagct gagcttctta gaattcttta attatgaaat 30900 ttactgtgac ttttctgctg attatttgca ccctatctgc gttttgttcc ccgacctcca 30960 agcctcaaag acatatatca tgcagattca ctcgtatatg gaatattcca agttgctaca 31020 atgaaaaaag cgatctttcc gaagcctggt tatatgcaat catctctgtt atggtgttct 31080 gcagtaccat cttagcccta gctatatatc cctaccttga cattggctgg aacgcaatag 31140 atgccatgaa ccacccaact ttccccgcgc ccgctatgct tccactgcaa caagttgttg 31200 ccggcggctt tgtcccagcc aatcagcctc gcccaccttc tcccaccccc actgaaatca 31260 gctactttaa tctaacagga ggagatgact gacaccctag atctagaaat ggacggaatt 31320 attacagagc agcgcctgct agaaagacgc agggcagcgg ccgagcaaca gcgcatgaat 31380 caagagctcc aagacatggt taacttgcac cagtgcaaaa ggggtatctt ttgtctggta 31440 aagcaggcca aagtcaccta cgacagtaat accaccggac accgccttag ctacaagttg 31500 ccaaccaagc gtcagaaatt ggtggtcatg gtgggagaaa agcccattac cataactcag 31560 cactcggtag aaaccgaagg ctgcattcac tcaccttgtc aaggacctga ggatctctgc 31620 acccttatta agaccctgtg cggtctcaaa gatcttattc cctttaacta ataaaaaaaa 31680 ataataaagc atcacttact taaaatcagt tagcaaattt ctgtccagtt tattcagcag 31740 cacctccttg ccctcctccc agctctggta ttgcagcttc ctcctggctg caaactttct 31800 ccacaatcta aatggaatgt cagtttcctc ctgttcctgt ccatccgcac ccactatctt 31860 catgttgttg cagatgaagc gcgcaagacc gtctgaagat accttcaacc ccgtgtatcc 31920 atatgacacg gaaaccggtc ctccaactgt gccttttctt actcctccct ttgtatcccc 31980 caatgggttt caagagagtc cccctggggt actctctttg cgcctatccg aacctctagt 32040 tacctccaat ggcatgcttg cgctcaaaat gggcaacggc ctctctctgg acgaggccgg 32100 caaccttacc tcccaaaatg taaccactgt gagcccacct ctcaaaaaaa ccaagtcaaa 32160 cataaacctg gaaatatctg cacccctcac agttacctca gaagccctaa ctgtggctgc 32220 cgccgcacct ctaatggtcg cgggcaacac actcaccatg caatcacagg ccccgctaac 32280 cgtgcacgac tccaaactta gcattgccac ccaaggaccc ctcacagtgt cagaaggaaa 32340 gctagccctg caaacatcag gccccctcac caccaccgat agcagtaccc ttactatcac 32400 tgcctcaccc cctctaacta ctgccactgg tagcttgggc attgacttga aagagcccat 32460 ttatacacaa aatggaaaac taggactaaa gtacggggct cctttgcatg taacagacga 32520 cctaaacact ttgaccgtag caactggtcc aggtgtgact attaataata cttccttgca 32580 aactaaagtt actggagcct tgggttttga ttcacaaggc aatatgcaac ttaatgtagc 32640 aggaggacta aggattgatt ctcaaaacag acgccttata cttgatgtta gttatccgtt 32700 tgatgctcaa aaccaactaa atctaagact aggacagggc cctcttttta taaactcagc 32760 ccacaacttg gatattaact acaacaaagg cctttacttg tttacagctt caaacaattc 32820 caaaaagctt gaggttaacc taagcactgc caaggggttg atgtttgacg ctacagccat 32880 agccattaat gcaggagatg ggcttgaatt tggttcacct aatgcaccaa acacaaatcc 32940 cctcaaaaca aaaattggcc atggcctaga atttgattca aacaaggcta tggttcctaa 33000 actaggaact ggccttagtt ttgacagcac aggtgccatt acagtaggaa acaaaaataa 33060 tgataagcta actttgtgga ccacaccagc tccatctcct aactgtagac taaatgcaga 33120 gaaagatgct aaactcactt tggtcttaac aaaatgtggc agtcaaatac ttgctacagt 33180 ttcagttttg gctgttaaag gcagtttggc tccaatatct ggaacagttc aaagtgctca 33240 tcttattata agatttgacg aaaatggagt gctactaaac aattccttcc tggacccaga 33300 atattggaac tttagaaatg gagatcttac tgaaggcaca gcctatacaa acgctgttgg 33360 atttatgcct aacctatcag cttatccaaa atctcacggt aaaactgcca aaagtaacat 33420 tgtcagtcaa gtttacttaa acggagacaa aactaaacct gtaacactaa ccattacact 33480 aaacggtaca caggaaacag gagacacaac tccaagtgca tactctatgt cattttcatg 33540 ggactggtct ggccacaact acattaatga aatatttgcc acatcctctt acactttttc 33600 atacattgcc caagaataaa gaatcgtttg tgttatgttt caacgtgttt atttttcaat 33660 tgcagaaaat ttcaagtcat ttttcattca gtagtatagc cccaccacca catagcttat 33720 acagatcacc gtacctcaac tttgtataat aaagttgtaa tcaaactcac agaaccctag 33780 tattcaacct gccacctccc tcccaacaca cagagtacac agtcctttct ccccggctgg 33840 ccttaaaaag catcatatca tgggtaacag acatattctt aggtgttata ttccacacgg 33900 tttcctgtcg agccaaacgc tcatcagtga tattaataaa ctccccgggc agctcactta 33960 agttcatgtc gctgtccagc tgctgagcca caggctgctg tccaacttgc ggttgcttaa 34020 cgggcggcga aggagaagtc cacgcctaca tgggggtaga gtcataatcg tgcatcagga 34080 tagggcggtg gtgctgcagc agcgcgcgaa taaactgctg ccgccgccgc tccgtcctgc 34140 aggaatacaa catggcagtg gtctcctcag cgatgattcg caccgcccgc agcataaggc 34200 gccttgtcct ccgggcacag cagcgcaccc tgatctcact taaatcagca cagtaactgc 34260 agcacagcac cacaatattg ttcaaaatcc cacagtgcaa ggcgctgtat ccaaagctca 34320 tggcggggac cacagaaccc acgtggccat cataccacaa gcgcaggtag attaagtggc 34380 gacccctcat aaacacgctg gacataaaca ttacctcttt tggcatgttg taattcacca 34440 cctcccggta ccatataaac ctctgattaa acatggcgcc atccaccacc atcctaaacc 34500 agctggccaa aacctgcccg ccggctatac actgcaggga accgggactg gaacaatgac 34560 agtggagagc ccaggactcg taaccatgga tcatcatgct cgtcatgata tcaatgttgg 34620 cacaacacag gcacacgtgc atacacttcc tcaggattac aagctcctcc cgcgttagaa 34680 ccatatccca gggaacaacc cattcctgaa tcagcgtaaa tcccacactg cagggaagac 34740 ctcgcacgta actcacgttg tgcattgtca aagtgttaca ttcgggcagc agcggatgat 34800 cctccagtat ggtagcgcgg gtttctgtct caaaaggagg tagacgatcc ctactgtacg 34860 gagtgcgccg agacaaccga gatcgtgttg gtcgtagtgt catgccaaat ggaacgccgg 34920 acgtagtcat atttcctgaa gcaaaaccag gtgcgggcgt gacaaacaga tctgcgtctc 34980 cggtctcgcc gcttagatcg ctctgtgtag tagttgtagt atatccactc tctcaaagca 35040 tccaggcgcc ccctggcttc gggttctatg taaactcctt catgcgccgc tgccctgata 35100 acatccacca ccgcagaata agccacaccc agccaaccta cacattcgtt ctgcgagtca 35160 cacacgggag gagcgggaag agctggaaga accatgtttt tttttttatt ccaaaagatt 35220 atccaaaacc tcaaaatgaa gatctattaa gtgaacgcgc tcccctccgg tggcgtggtc 35280 aaactctaca gccaaagaac agataatggc atttgtaaga tgttgcacaa tggcttccaa 35340 aaggcaaacg gccctcacgt ccaagtggac gtaaaggcta aacccttcag ggtgaatctc 35400 ctctataaac attccagcac cttcaaccat gcccaaataa ttctcatctc gccaccttct 35460 caatatatct ctaagcaaat cccgaatatt aagtccggcc attgtaaaaa tctgctccag 35520 agcgccctcc accttcagcc tcaagcagcg aatcatgatt gcaaaaattc aggttcctca 35580 cagacctgta taagattcaa aagcggaaca ttaacaaaaa taccgcgatc ccgtaggtcc 35640 cttcgcaggg ccagctgaac ataatcgtgc aggtctgcac ggaccagcgc ggccacttcc 35700 ccgccaggaa ccatgacaaa agaacccaca ctgattatga cacgcatact cggagctatg 35760 ctaaccagcg tagccccgat gtaagcttgt tgcatgggcg gcgatataaa atgcaaggtg 35820 ctgctcaaaa aatcaggcaa agcctcgcgc aaaaaagaaa gcacatcgta gtcatgctca 35880 tgcagataaa ggcaggtaag ctccggaacc accacagaaa aagacaccat ttttctctca 35940 aacatgtctg cgggtttctg cataaacaca aaataaaata acaaaaaaac atttaaacat 36000 tagaagcctg tcttacaaca ggaaaaacaa cccttataag cataagacgg actacggcca 36060 tgccggcgtg accgtaaaaa aactggtcac cgtgattaaa aagcaccacc gacagctcct 36120 cggtcatgtc cggagtcata atgtaagact cggtaaacac atcaggttga ttcacatcgg 36180 tcagtgctaa aaagcgaccg aaatagcccg ggggaataca tacccgcagg cgtagagaca 36240 acattacagc ccccatagga ggtataacaa aattaatagg agagaaaaac acataaacac 36300 ctgaaaaacc ctcctgccta ggcaaaatag caccctcccg ctccagaaca acatacagcg 36360 cttccacagc ggcagccata acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa 36420 aacaccactc gacacggcac cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag 36480 agcgagtata tataggacta aaaaatgacg taacggttaa agtccacaaa aaacacccag 36540 aaaaccgcac gcgaacctac gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa 36600 tcgtcacttc cgttttccca cgttacgtca cttcccattt taagaaaact acaattccca 36660 acacatacaa gttactccgc cctaaaacct acgtcacccg ccccgttccc acgccccgcg 36720 ccacgtcaca aactccaccc cctcattatc atattggctt caatccaaaa taaggtatat 36780 tattgatgat g 36791 <210> 2 <211> 36791 <212> DNA <213> Artificial Sequence <220> <223> Synthetic adenovirus genome E1A-P2A-YPet <400> 2 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420 cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480 tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540 tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600 aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660 tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720 cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780 gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840 cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900 ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960 cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020 caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080 ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140 tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200 gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260 ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320 cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380 ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440 gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500 cctgggcaac ctttggactt gagctgtaaa cgccccaggc caggaagcgg agctactaac 1560 ttcagcctgc tgaagcaggc tggtgacgtc gaggagaatc ctggcccaat ggtgagcaaa 1620 ggcgaagagc tgttcaccgg cgtggtgccc atcctggtgg agctggacgg cgacgtgaac 1680 ggccacaagt tcagcgtgag cggcgagggc gagggcgacg ccacctacgg caagctgacc 1740 ctgaagctgc tgtgcaccac cggcaagctg cccgtgccct ggcccaccct ggtgaccacc 1800 ctgggctacg gcgtgcagtg cttcgcccgg taccccgacc acatgaagca gcacgacttc 1860 ttcaagagcg ccatgcccga gggctacgtg caggagcgga ccatcttctt caaggacgac 1920 ggcaactaca agacccgggc cgaggtgaag ttcgagggcg acaccctggt gaaccggatc 1980 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tgggccacaa gctggagtac 2040 aactacaaca gccacaacgt gtacatcacc gccgacaagc agaagaacgg catcaaggcc 2100 aacttcaaga tccggcacaa catcgaggac ggcggcgtgc agctggccga ccactaccag 2160 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac 2220 cagagcgccc tgttcaagga ccccaacgag aagcgggacc acatggtgct gctggagttc 2280 ctgaccgccg ccggcatcac cgagggcatg aacgagctct ataagtaagg tgtaaacctg 2340 tgattgcgtg tgtggttaac gcctttgttt gctgaatgag ttgatgtaag tttaataaag 2400 ggtgagataa tgtttaactt gcatggcgtg ttaaatgggg cggggcttaa agggtatata 2460 atgcgccgtg ggctaatctt ggttacatct gacctcatgg aggcttggga gtgtttggaa 2520 gatttttctg ctgtgcgtaa cttgctggaa cagagctcta acagtacctc ttggttttgg 2580 aggtttctgt ggggctcatc ccaggcaaag ttagtctgca gaattaagga ggattacaag 2640 tgggaatttg aagagctttt gaaatcctgt ggtgagctgt ttgattcttt gaatctgggt 2700 caccaggcgc ttttccaaga gaaggtcatc aagactttgg atttttccac accggggcgc 2760 gctgcggctg ctgttgcttt tttgagtttt ataaaggata aatggagcga agaaacccat 2820 ctgagcgggg ggtacctgct ggattttctg gccatgcatc tgtggagagc ggttgtgaga 2880 cacaagaatc gcctgctact gttgtcttcc gtccgcccgg cgataatacc gacggaggag 2940 cagcagcagc agcaggagga agccaggcgg cggcggcagg agcagagccc atggaacccg 3000 agagccggcc tggaccctcg ggaatgaatg ttgtacaggt ggctgaactg tatccagaac 3060 tgagacgcat tttgacaatt acagaggatg ggcaggggct aaagggggta aagagggagc 3120 ggggggcttg tgaggctaca gaggaggcta ggaatctagc ttttagctta atgaccagac 3180 accgtcctga gtgtattact tttcaacaga tcaaggataa ttgcgctaat gagcttgatc 3240 tgctggcgca gaagtattcc atagagcagc tgaccactta ctggctgcag ccaggggatg 3300 attttgagga ggctattagg gtatatgcaa aggtggcact taggccagat tgcaagtaca 3360 agatcagcaa acttgtaaat atcaggaatt gttgctacat ttctgggaac ggggccgagg 3420 tggagataga tacggaggat agggtggcct ttagatgtag catgataaat atgtggccgg 3480 gggtgcttgg catggacggg gtggttatta tgaatgtaag gtttactggc cccaatttta 3540 gcggtacggt tttcctggcc aataccaacc ttatcctaca cggtgtaagc ttctatgggt 3600 ttaacaatac ctgtgtggaa gcctggaccg atgtaagggt tcggggctgt gccttttact 3660 gctgctggaa gggggtggtg tgtcgcccca aaagcagggc ttcaattaag aaatgcctct 3720 ttgaaaggtg taccttgggt atcctgtctg agggtaactc cagggtgcgc cacaatgtgg 3780 cctccgactg tggttgcttc atgctagtga aaagcgtggc tgtgattaag cataacatgg 3840 tatgtggcaa ctgcgaggac agggcctctc agatgctgac ctgctcggac ggcaactgtc 3900 acctgctgaa gaccattcac gtagccagcc actctcgcaa ggcctggcca gtgtttgagc 3960 ataacatact gacccgctgt tccttgcatt tgggtaacag gaggggggtg ttcctacctt 4020 accaatgcaa tttgagtcac actaagatat tgcttgagcc cgagagcatg tccaaggtga 4080 acctgaacgg ggtgtttgac atgaccatga agatctggaa ggtgctgagg tacgatgaga 4140 cccgcaccag gtgcagaccc tgcgagtgtg gcggtaaaca tattaggaac cagcctgtga 4200 tgctggatgt gaccgaggag ctgaggcccg atcacttggt gctggcctgc acccgcgctg 4260 agtttggctc tagcgatgaa gatacagatt gaggtactga aatgtgtggg cgtggcttaa 4320 gggtgggaaa gaatatataa ggtgggggtc ttatgtagtt ttgtatctgt tttgcagcag 4380 ccgccgccgc ccaacttttc tatacaaagt tgtaatgagc accaactcgt ttgatggaag 4440 cattgtgagc tcatatttga caacgcgcat gcccccatgg gccggggtgc gtcagaatgt 4500 gatgggctcc agcattgatg gtcgccccgt cctgcccgca aactctacta ccttgaccta 4560 cgagaccgtg tctggaacgc cgttggagac tgcagcctcc gccgccgctt cagccgctgc 4620 agccaccgcc cgcgggattg tgactgactt tgctttcctg agcccgcttg caagcagtgc 4680 agcttcccgt tcatccgccc gcgatgacaa gttgacggct cttttggcac aattggattc 4740 tttgacccgg gaacttaatg tcgtttctca gcagctgttg gatctgcgcc agcaggtttc 4800 tgccctgaag gcttcctccc ctcccaatgc ggtttaaaac ataaataaaa aaccagactc 4860 tgtttggatt tggatcaagc ataagtgtct tgctgtcttt atttaggggt tttgcgcgcg 4920 cggtaggccc gggaccagcg gtctcggtcg ttgagggtcc tgtgtatttt ttccaggacg 4980 tggtaaaggt gactctggat gttcagatac atgggcataa gcccgtctct ggggtggagg 5040 tagcaccact gcagagcttc atgctgcggg gtggtgttgt agatgatcca gtcgtagcag 5100 gagcgctggg cgtggtgcct aaaaatgtct ttcagtagca agctgattgc caggggcagg 5160 cccttggtgt aagtgtttac aaagcggtta agctgggatg ggtgcatacg tggggatatg 5220 agatgcatct tggactgtat ttttaggttg gctatgttcc cagccatatc cctccgggga 5280 ttcatgttgt gcagaaccac cagcacagtg tatccggtgc acttgggaaa tttgtcatgt 5340 agcttagaag gaaatgcgtg gaagaacttg gagacgccct tgtgacctcc aagattttcc 5400 atgcattcgt ccataatgat ggcaatgggc ccacgggcgg cggcctgggc gaagatattt 5460 ctgggatcac taacgtcata gttgtgttcc aggatgagat cgtcataggc catttttaca 5520 aagcgcgggc ggagggtgcc agactgcggt ataatggttc catccggccc aggggcgtag 5580 ttaccctcac agatttgcat ttcccacgct ttgagttcag atggggggat catgtctacc 5640 tgcggggcga tgaagaaaac ggtttccggg gtaggggaga tcagctggga agaaagcagg 5700 ttcctgagca gctgcgactt accgcagccg gtgggcccgt aaatcacacc tattaccggg 5760 tgcaactggt agttaagaga gctgcagctg ccgtcatccc tgagcagggg ggccacttcg 5820 ttaagcatgt ccctgactcg catgttttcc ctgaccaaat ccgccagaag gcgctcgccg 5880 cccagcgata gcagttcttg caaggaagca aagtttttca acggtttgag accgtccgcc 5940 gtaggcatgc ttttgagcgt ttgaccaagc agttccaggc ggtcccacag ctcggtcacc 6000 tgctctacgg catctcgatc cagcatatct cctcgtttcg cgggttgggg cggctttcgc 6060 tgtacggcag tagtcggtgc tcgtccagac gggccagggt catgtctttc cacgggcgca 6120 gggtcctcgt cagcgtagtc tgggtcacgg tgaaggggtg cgctccgggc tgcgcgctgg 6180 ccagggtgcg cttgaggctg gtcctgctgg tgctgaagcg ctgccggtct tcgccctgcg 6240 cgtcggccag gtagcatttg accatggtgt catagtccag cccctccgcg gcgtggccct 6300 tggcgcgcag cttgcccttg gaggaggcgc cgcacgaggg gcagtgcaga cttttgaggg 6360 cgtagagctt gggcgcgaga aataccgatt ccggggagta ggcatccgcg ccgcaggccc 6420 cgcagacggt ctcgcattcc acgagccagg tgagctctgg ccgttcgggg tcaaaaacca 6480 ggtttccccc atgctttttg atgcgtttct tacctctggt ttccatgagc cggtgtccac 6540 gctcggtgac gaaaaggctg tccgtgtccc cgtatacaga cttgagaggc ctgtcctcga 6600 gcggtgttcc gcggtcctcc tcgtatagaa actcggacca ctctgagaca aaggctcgcg 6660 tccaggccag cacgaaggag gctaagtggg aggggtagcg gtcgttgtcc actagggggt 6720 ccactcgctc cagggtgtga agacacatgt cgccctcttc ggcatcaagg aaggtgattg 6780 gtttgtaggt gtaggccacg tgaccgggtg ttcctgaagg ggggctataa aagggggtgg 6840 gggcgcgttc gtcctcactc tcttccgcat cgctgtctgc gagggccagc tgttggggtg 6900 agtactccct ctgaaaagcg ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg 6960 aggaggattt gatattcacc tggcccgcgg tgatgccttt gagggtggcc gcatccatct 7020 ggtcagaaaa gacaatcttt ttgttgtcaa gcttggtggc aaacgacccg tagagggcgt 7080 tggacagcaa cttggcgatg gagcgcaggg tttggttttt gtcgcgatcg gcgcgctcct 7140 tggccgcgat gtttagctgc acgtattcgc gcgcaacgca ccgccattcg ggaaagacgg 7200 tggtgcgctc gtcgggcacc aggtgcacgc gccaaccgcg gttgtgcagg gtgacaaggt 7260 caacgctggt ggctacctct ccgcgtaggc gctcgttggt ccagcagagg cggccgccct 7320 tgcgcgagca gaatggcggt agggggtcta gctgcgtctc gtccgggggg tctgcgtcca 7380 cggtaaagac cccgggcagc aggcgcgcgt cgaagtagtc tatcttgcat ccttgcaagt 7440 ctagcgcctg ctgccatgcg cgggcggcaa gcgcgcgctc gtatgggttg agtgggggac 7500 cccatggcat ggggtgggtg agcgcggagg cgtacatgcc gcaaatgtcg taaacgtaga 7560 ggggctctct gagtattcca agatatgtag ggtagcatct tccaccgcgg atgctggcgc 7620 gcacgtaatc gtatagttcg tgcgagggag cgaggaggtc gggaccgagg ttgctacggg 7680 cgggctgctc tgctcggaag actatctgcc tgaagatggc atgtgagttg gatgatatgg 7740 ttggacgctg gaagacgttg aagctggcgt ctgtgagacc taccgcgtca cgcacgaagg 7800 aggcgtagga gtcgcgcagc ttgttgacca gctcggcggt gacctgcacg tctagggcgc 7860 agtagtccag ggtttccttg atgatgtcat acttatcctg tccctttttt ttccacagct 7920 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg 7980 cctccgaacg gtaagagcct agcatgtaga actggttgac ggcctggtag gcgcagcatc 8040 ccttttctac gggtagcgcg tatgcctgcg cggccttccg gagcgaggtg tgggtgagcg 8100 caaaggtgtc cctgaccatg actttgaggt actggtattt gaagtcagtg tcgtcgcatc 8160 cgccctgctc ccagagcaaa aagtccgtgc gctttttgga acgcggattt ggcagggcga 8220 aggtgacatc gttgaagagt atctttcccg cgcgaggcat aaagttgcgt gtgatgcgga 8280 agggtcccgg cacctcggaa cggttgttaa ttacctgggc ggcgagcacg atctcgtcaa 8340 agccgttgat gttgtggccc acaatgtaaa gttccaagaa gcgcgggatg cccttgatgg 8400 aaggcaattt tttaagttcc tcgtaggtga gctcttcagg ggagctgagc ccgtgctctg 8460 aaagggccca gtctgcaaga tgagggttgg aagcgacgaa tgagctccac aggtcacggg 8520 ccattagcat ttgcaggtgg tcgcgaaagg tcctaaactg gcgacctatg gccatttttt 8580 ctggggtgat gcagtagaag gtaagcgggt cttgttccca gcggtcccat ccaaggttcg 8640 cggctaggtc tcgcgcggca gtcactagag gctcatctcc gccgaacttc atgaccagca 8700 tgaagggcac gagctgcttc ccaaaggccc ccatccaagt ataggtctct acatcgtagg 8760 tgacaaagag acgctcggtg cgaggatgcg agccgatcgg gaagaactgg atctcccgcc 8820 accaattgga ggagtggcta ttgatgtggt gaaagtagaa gtccctgcga cgggccgaac 8880 actcgtgctg gcttttgtaa aaacgtgcgc agtactggca gcggtgcacg ggctgtacat 8940 cctgcacgag gttgacctga cgaccgcgca caaggaagca gagtgggaat ttgagcccct 9000 cgcctggcgg gtttggctgg tggtcttcta cttcggctgc ttgtccttga ccgtctggct 9060 gctcgagggg agttacggtg gatcggacca ccacgccgcg cgagcccaaa gtccagatgt 9120 ccgcgcgcgg cggtcggagc ttgatgacaa catcgcgcag atgggagctg tccatggtct 9180 ggagctcccg cggcgtcagg tcaggcggga gctcctgcag gtttacctcg catagacggg 9240 tcagggcgcg ggctagatcc aggtgatacc taatttccag gggctggttg gtggcggcgt 9300 cgatggcttg caagaggccg catccccgcg gcgcgactac ggtaccgcgc ggcgggcggt 9360 gggccgcggg ggtgtccttg gatgatgcat ctaaaagcgg tgacgcgggc gagcccccgg 9420 aggtaggggg ggctccggac ccgccgggag agggggcagg ggcacgtcgg cgccgcgcgc 9480 gggcaggagc tggtgctgcg cgcgtaggtt gctggcgaac gcgacgacgc ggcggttgat 9540 ctcctgaatc tggcgcctct gcgtgaagac gacgggcccg gtgagcttga gcctgaaaga 9600 gagttcgaca gaatcaattt cggtgtcgtt gacggcggcc tggcgcaaaa tctcctgcac 9660 gtctcctgag ttgtcttgat aggcgatctc ggccatgaac tgctcgatct cttcctcctg 9720 gagatctccg cgtccggctc gctccacggt ggcggcgagg tcgttggaaa tgcgggccat 9780 gagctgcgag aaggcgttga ggcctccctc gttccagacg cggctgtaga ccacgccccc 9840 ttcggcatcg cgggcgcgca tgaccacctg cgcgagattg agctccacgt gccgggcgaa 9900 gacggcgtag tttcgcaggc gctgaaagag gtagttgagg gtggtggcgg tgtgttctgc 9960 cacgaagaag tacataaccc agcgtcgcaa cgtggattcg ttgatatccc ccaaggcctc 10020 aaggcgctcc atggcctcgt agaagtccac ggcgaagttg aaaaactggg agttgcgcgc 10080 cgacacggtt aactcctcct ccagaagacg gatgagctcg gcgacagtgt cgcgcacctc 10140 gcgctcaaag gctacagggg cctcttcttc ttcttcaatc tcctcttcca taagggcctc 10200 cccttcttct tcttctggcg gcggtggggg aggggggaca cggcggcgac gacggcgcac 10260 cgggaggcgg tcgacaaagc gctcgatcat ctccccgcgg cgacggcgca tggtctcggt 10320 gacggcgcgg ccgttctcgc gggggcgcag ttggaagacg ccgcccgtca tgtcccggtt 10380 atgggttggc ggggggctgc catgcggcag ggatacggcg ctaacgatgc atctcaacaa 10440 ttgttgtgta ggtactccgc cgccgaggga cctgagcgag tccgcatcga ccggatcgga 10500 aaacctctcg agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc 10560 gggcggcagc gggcggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt 10620 aaagtaggcg gtcttgagac ggcggatggt cgacagaagc accatgtcct tgggtccggc 10680 ctgctgaatg cgcaggcggt cggccatgcc ccaggcttcg ttttgacatc ggcgcaggtc 10740 tttgtagtag tcttgcatga gcctttctac cggcacttct tcttctcctt cctcttgtcc 10800 tgcatctctt gcatctatcg ctgcggcggc ggcggagttt ggccgtaggt ggcgccctct 10860 tcctcccatg cgtgtgaccc cgaagcccct catcggctga agcagggcta ggtcggcgac 10920 aacgcgctcg gctaatatgg cctgctgcac ctgcgtgagg gtagactgga agtcatccat 10980 gtccacaaag cggtggtatg cgcccgtgtt gatggtgtaa gtgcagttgg ccataacgga 11040 ccagttaacg gtctggtgac ccggctgcga gagctcggtg tacctgagac gcgagtaagc 11100 cctcgagtca aatacgtagt cgttgcaagt ccgcaccagg tactggtatc ccaccaaaaa 11160 gtgcggcggc ggctggcggt agaggggcca gcgtagggtg gccggggctc cgggggcgag 11220 atcttccaac ataaggcgat gatatccgta gatgtacctg gacatccagg tgatgccggc 11280 ggcggtggtg gaggcgcgcg gaaagtcgcg gacgcggttc cagatgttgc gcagcggcaa 11340 aaagtgctcc atggtcggga cgctctggcc ggtcaggcgc gcgcaatcgt tgacgctcta 11400 gaccgtgcaa aaggagagcc tgtaagcggg cactcttccg tggtctggtg gataaattcg 11460 caagggtatc atggcggacg accggggttc gagccccgta tccggccgtc cgccgtgatc 11520 catgcggtta ccgcccgcgt gtcgaaccca ggtgtgcgac gtcagacaac gggggagtgc 11580 tccttttggc ttccttccag gcgcggcggc tgctgcgcta gcttttttgg ccactggccg 11640 cgcgcagcgt aagcggttag gctggaaagc gaaagcatta agtggctcgc tccctgtagc 11700 cggagggtta ttttccaagg gttgagtcgc gggacccccg gttcgagtct cggaccggcc 11760 ggactgcggc gaacgggggt ttgcctcccc gtcatgcaag accccgcttg caaattcctc 11820 cggaaacagg gacgagcccc ttttttgctt ttcccagatg catccggtgc tgcggcagat 11880 gcgcccccct cctcagcagc ggcaagagca agagcagcgg cagacatgca gggcaccctc 11940 ccctcctcct accgcgtcag gaggggcgac atccgcggtt gacgcggcag cagatggtga 12000 ttacgaaccc ccgcggcgcc gggcccggca ctacctggac ttggaggagg gcgagggcct 12060 ggcgcggcta ggagcgccct ctcctgagcg gtacccaagg gtgcagctga agcgtgatac 12120 gcgtgaggcg tacgtgccgc ggcagaacct gtttcgcgac cgcgagggag aggagcccga 12180 ggagatgcgg gatcgaaagt tccacgcagg gcgcgagctg cggcatggcc tgaatcgcga 12240 gcggttgctg cgcgaggagg actttgagcc cgacgcgcga accgggatta gtcccgcgcg 12300 cgcacacgtg gcggccgccg acctggtaac cgcatacgag cagacggtga accaggagat 12360 taactttcaa aaaagcttta acaaccacgt gcgtacgctt gtggcgcgcg aggaggtggc 12420 tataggactg atgcatctgt gggactttgt aagcgcgctg gagcaaaacc caaatagcaa 12480 gccgctcatg gcgcagctgt tccttatagt gcagcacagc agggacaacg aggcattcag 12540 ggatgcgctg ctaaacatag tagagcccga gggccgctgg ctgctcgatt tgataaacat 12600 cctgcagagc atagtggtgc aggagcgcag cttgagcctg gctgacaagg tggccgccat 12660 caactattcc atgcttagcc tgggcaagtt ttacgcccgc aagatatacc atacccctta 12720 cgttcccata gacaaggagg taaagatcga ggggttctac atgcgcatgg cgctgaaggt 12780 gcttaccttg agcgacgacc tgggcgttta tcgcaacgag cgcatccaca aggccgtgag 12840 cgtgagccgg cggcgcgagc tcagcgaccg cgagctgatg cacagcctgc aaagggccct 12900 ggctggcacg ggcagcggcg atagagaggc cgagtcctac tttgacgcgg gcgctgacct 12960 gcgctgggcc ccaagccgac gcgccctgga ggcagctggg gccggacctg ggctggcggt 13020 ggcacccgcg cgcgctggca acgtcggcgg cgtggaggaa tatgacgagg acgatgagta 13080 cgagccagag gacggcgagt actaagcggt gatgtttctg atcagatgat gcaagacgca 13140 acggacccgg cggtgcgggc ggcgctgcag agccagccgt ccggccttaa ctccacggac 13200 gactggcgcc aggtcatgga ccgcatcatg tcgctgactg cgcgcaatcc tgacgcgttc 13260 cggcagcagc cgcaggccaa ccggctctcc gcaattctgg aagcggtggt cccggcgcgc 13320 gcaaacccca cgcacgagaa ggtgctggcg atcgtaaacg cgctggccga aaacagggcc 13380 atccggcccg acgaggccgg cctggtctac gacgcgctgc ttcagcgcgt ggctcgttac 13440 aacagcggca acgtgcagac caacctggac cggctggtgg gggatgtgcg cgaggccgtg 13500 gcgcagcgtg agcgcgcgca gcagcagggc aacctgggct ccatggttgc actaaacgcc 13560 ttcctgagta cacagcccgc caacgtgccg cggggacagg aggactacac caactttgtg 13620 agcgcactgc ggctaatggt gactgagaca ccgcaaagtg aggtgtacca gtctgggcca 13680 gactattttt tccagaccag tagacaaggc ctgcagaccg taaacctgag ccaggctttc 13740 aaaaacttgc aggggctgtg gggggtgcgg gctcccacag gcgaccgcgc gaccgtgtct 13800 agcttgctga cgcccaactc gcgcctgttg ctgctgctaa tagcgccctt cacggacagt 13860 ggcagcgtgt cccgggacac atacctaggt cacttgctga cactgtaccg cgaggccata 13920 ggtcaggcgc atgtggacga gcatactttc caggagatta caagtgtcag ccgcgcgctg 13980 gggcaggagg acacgggcag cctggaggca accctaaact acctgctgac caaccggcgg 14040 cagaagatcc cctcgttgca cagtttaaac agcgaggagg agcgcatttt gcgctacgtg 14100 cagcagagcg tgagccttaa cctgatgcgc gacggggtaa cgcccagcgt ggcgctggac 14160 atgaccgcgc gcaacatgga accgggcatg tatgcctcaa accggccgtt tatcaaccgc 14220 ctaatggact acttgcatcg cgcggccgcc gtgaaccccg agtatttcac caatgccatc 14280 ttgaacccgc actggctacc gccccctggt ttctacaccg ggggattcga ggtgcccgag 14340 ggtaacgatg gattcctctg ggacgacata gacgacagcg tgttttcccc gcaaccgcag 14400 accctgctag agttgcaaca gcgcgagcag gcagaggcgg cgctgcgaaa ggaaagcttc 14460 cgcaggccaa gcagcttgtc cgatctaggc gctgcggccc cgcggtcaga tgctagtagc 14520 ccatttccaa gcttgatagg gtctcttacc agcactcgca ccacccgccc gcgcctgctg 14580 ggcgaggagg agtacctaaa caactcgctg ctgcagccgc agcgcgaaaa aaacctgcct 14640 ccggcatttc ccaacaacgg gatagagagc ctagtggaca agatgagtag atggaagacg 14700 tacgcgcagg agcacaggga cgtgccaggc ccgcgcccgc ccacccgtcg tcaaaggcac 14760 gaccgtcagc ggggtctggt gtgggaggac gatgactcgg cagacgacag cagcgtcctg 14820 gatttgggag ggagtggcaa cccgtttgcg caccttcgcc ccaggctggg gagaatgttt 14880 taaaaaaaaa aaagcatgat gcaaaataaa aaactcacca aggccatggc accgagcgtt 14940 ggttttcttg tattcccctt agtatgcggc gcgcggcgat gtatgaggaa ggtcctcctc 15000 cctcctacga gagtgtggtg agcgcggcgc cagtggcggc ggcgctgggt tctcccttcg 15060 atgctcccct ggacccgccg tttgtgcctc cgcggtacct gcggcctacc ggggggagaa 15120 acagcatccg ttactctgag ttggcacccc tattcgacac cacccgtgtg tacctggtgg 15180 acaacaagtc aacggatgtg gcatccctga actaccagaa cgaccacagc aactttctga 15240 ccacggtcat tcaaaacaat gactacagcc cgggggaggc aagcacacag accatcaatc 15300 ttgacgaccg gtcgcactgg ggcggcgacc tgaaaaccat cctgcatacc aacatgccaa 15360 atgtgaacga gttcatgttt accaataagt ttaaggcgcg ggtgatggtg tcgcgcttgc 15420 ctactaagga caatcaggtg gagctgaaat acgagtgggt ggagttcacg ctgcccgagg 15480 gcaactactc cgagaccatg accatagacc ttatgaacaa cgcgatcgtg gagcactact 15540 tgaaagtggg cagacagaac ggggttctgg aaagcgacat cggggtaaag tttgacaccc 15600 gcaacttcag actggggttt gaccccgtca ctggtcttgt catgcctggg gtatatacaa 15660 acgaagcctt ccatccagac atcattttgc tgccaggatg cggggtggac ttcacccaca 15720 gccgcctgag caacttgttg ggcatccgca agcggcaacc cttccaggag ggctttagga 15780 tcacctacga tgatctggag ggtggtaaca ttcccgcact gttggatgtg gacgcctacc 15840 aggcgagctt gaaagatgac accgaacagg gcgggggtgg cgcaggcggc agcaacagca 15900 gtggcagcgg cgcggaagag aactccaacg cggcagccgc ggcaatgcag ccggtggagg 15960 acatgaacga tcatgccatt cgcggcgaca cctttgccac acgggctgag gagaagcgcg 16020 ctgaggccga agcagcggcc gaagctgccg cccccgctgc gcaacccgag gtcgagaagc 16080 ctcagaagaa accggtgatc aaacccctga cagaggacag caagaaacgc agttacaacc 16140 taataagcaa tgacagcacc ttcacccagt accgcagctg gtaccttgca tacaactacg 16200 gcgaccctca gaccggaatc cgctcatgga ccctgctttg cactcctgac gtaacctgcg 16260 gctcggagca ggtctactgg tcgttgccag acatgatgca agaccccgtg accttccgct 16320 ccacgcgcca gatcagcaac tttccggtgg tgggcgccga gctgttgccc gtgcactcca 16380 agagcttcta caacgaccag gccgtctact cccaactcat ccgccagttt acctctctga 16440 cccacgtgtt caatcgcttt cccgagaacc agattttggc gcgcccgcca gcccccacca 16500 tcaccaccgt cagtgaaaac gttcctgctc tcacagatca cgggacgcta ccgctgcgca 16560 acagcatcgg aggagtccag cgagtgacca ttactgacgc cagacgccgc acctgcccct 16620 acgtttacaa ggccctgggc atagtctcgc cgcgcgtcct atcgagccgc actttttgag 16680 caagcatgtc catccttata tcgcccagca ataacacagg ctggggcctg cgcttcccaa 16740 gcaagatgtt tggcggggcc aagaagcgct ccgaccaaca cccagtgcgc gtgcgcgggc 16800 actaccgcgc gccctggggc gcgcacaaac gcggccgcac tgggcgcacc accgtcgatg 16860 acgccatcga cgcggtggtg gaggaggcgc gcaactacac gcccacgccg ccaccagtgt 16920 ccacagtgga cgcggccatt cagaccgtgg tgcgcggagc ccggcgctat gctaaaatga 16980 agagacggcg gaggcgcgta gcacgtcgcc accgccgccg acccggcact gccgcccaac 17040 gcgcggcggc ggccctgctt aaccgcgcac gtcgcaccgg ccgacgggcg gccatgcggg 17100 ccgctcgaag gctggccgcg ggtattgtca ctgtgccccc caggtccagg cgacgagcgg 17160 ccgccgcagc agccgcggcc attagtgcta tgactcaggg tcgcaggggc aacgtgtatt 17220 gggtgcgcga ctcggttagc ggcctgcgcg tgcccgtgcg cacccgcccc ccgcgcaact 17280 agattgcaag aaaaaactac ttagactcgt actgttgtat gtatccagcg gcggcggcgc 17340 gcaacgaagc tatgtccaag cgcaaaatca aagaagagat gctccaggtc atcgcgccgg 17400 agatctatgg ccccccgaag aaggaagagc aggattacaa gccccgaaag ctaaagcggg 17460 tcaaaaagaa aaagaaagat gatgatgatg aacttgacga cgaggtggaa ctgctgcacg 17520 ctaccgcgcc caggcgacgg gtacagtgga aaggtcgacg cgtaaaacgt gttttgcgac 17580 ccggcaccac cgtagtcttt acgcccggtg agcgctccac ccgcacctac aagcgcgtgt 17640 atgatgaggt gtacggcgac gaggacctgc ttgagcaggc caacgagcgc ctcggggagt 17700 ttgcctacgg aaagcggcat aaggacatgc tggcgttgcc gctggacgag ggcaacccaa 17760 cacctagcct aaagcccgta acactgcagc aggtgctgcc cgcgcttgca ccgtccgaag 17820 aaaagcgcgg cctaaagcgc gagtctggtg acttggcacc caccgtgcag ctgatggtac 17880 ccaagcgcca gcgactggaa gatgtcttgg aaaaaatgac cgtggaacct gggctggagc 17940 ccgaggtccg cgtgcggcca atcaagcagg tggcgccggg actgggcgtg cagaccgtgg 18000 acgttcagat acccactacc agtagcacca gtattgccac cgccacagag ggcatggaga 18060 cacaaacgtc cccggttgcc tcagcggtgg cggatgccgc ggtgcaggcg gtcgctgcgg 18120 ccgcgtccaa gacctctacg gaggtgcaaa cggacccgtg gatgtttcgc gtttcagccc 18180 cccggcgccc gcgcggttcg aggaagtacg gcgccgccag cgcgctactg cccgaatatg 18240 ccctacatcc ttccattgcg cctacccccg gctatcgtgg ctacacctac cgccccagaa 18300 gacgagcaac tacccgacgc cgaaccacca ctggaacccg ccgccgccgt cgccgtcgcc 18360 agcccgtgct ggccccgatt tccgtgcgca gggtggctcg cgaaggaggc aggaccctgg 18420 tgctgccaac agcgcgctac caccccagca tcgtttaaaa gccggtcttt gtggttcttg 18480 cagatatggc cctcacctgc cgcctccgtt tcccggtgcc gggattccga ggaagaatgc 18540 accgtaggag gggcatggcc ggccacggcc tgacgggcgg catgcgtcgt gcgcaccacc 18600 ggcggcggcg cgcgtcgcac cgtcgcatgc gcggcggtat cctgcccctc cttattccac 18660 tgatcgccgc ggcgattggc gccgtgcccg gaattgcatc cgtggccttg caggcgcaga 18720 gacactgatt aaaaacaagt tgcatgtgga aaaatcaaaa taaaaagtct ggactctcac 18780 gctcgcttgg tcctgtaact attttgtaga atggaagaca tcaactttgc gtctctggcc 18840 ccgcgacacg gctcgcgccc gttcatggga aactggcaag atatcggcac cagcaatatg 18900 agcggtggcg ccttcagctg gggctcgctg tggagcggca ttaaaaattt cggttccacc 18960 gttaagaact atggcagcaa ggcctggaac agcagcacag gccagatgct gagggataag 19020 ttgaaagagc aaaatttcca acaaaaggtg gtagatggcc tggcctctgg cattagcggg 19080 gtggtggacc tggccaacca ggcagtgcaa aataagatta acagtaagct tgatccccgc 19140 cctcccgtag aggagcctcc accggccgtg gagacagtgt ctccagaggg gcgtggcgaa 19200 aagcgtccgc gccccgacag ggaagaaact ctggtgacgc aaatagacga gcctccctcg 19260 tacgaggagg cactaaagca aggcctgccc accacccgtc ccatcgcgcc catggctacc 19320 ggagtgctgg gccagcacac acccgtaacg ctggacctgc ctccccccgc cgacacccag 19380 cagaaacctg tgctgccagg cccgaccgcc gttgttgtaa cccgtcctag ccgcgcgtcc 19440 ctgcgccgcg ccgccagcgg tccgcgatcg ttgcggcccg tagccagtgg caactggcaa 19500 agcacactga acagcatcgt gggtctgggg gtgcaatccc tgaagcgccg acgatgcttc 19560 tgaatagcta acgtgtcgta tgtgtgtcat gtatgcgtcc atgtcgccgc cagaggagct 19620 gctgagccgc cgcgcgcccg ctttccaaga tggctacccc ttcgatgatg ccgcagtggt 19680 cttacatgca catctcgggc caggacgcct cggagtacct gagccccggg ctggtgcagt 19740 ttgcccgcgc caccgagacg tacttcagcc tgaataacaa gtttagaaac cccacggtgg 19800 cgcctacgca cgacgtgacc acagaccggt cccagcgttt gacgctgcgg ttcatccctg 19860 tggaccgtga ggatactgcg tactcgtaca aggcgcggtt caccctagct gtgggtgata 19920 accgtgtgct ggacatggct tccacgtact ttgacatccg cggcgtgctg gacaggggcc 19980 ctacttttaa gccctactct ggcactgcct acaacgccct ggctcccaag ggtgccccaa 20040 atccttgcga atgggatgaa gctgctactg ctcttgaaat aaacctagaa gaagaggacg 20100 atgacaacga agacgaagta gacgagcaag ctgagcagca aaaaactcac gtatttgggc 20160 aggcgcctta ttctggtata aatattacaa aggagggtat tcaaataggt gtcgaaggtc 20220 aaacacctaa atatgccgat aaaacatttc aacctgaacc tcaaatagga gaatctcagt 20280 ggtacgaaac tgaaattaat catgcagctg ggagagtcct taaaaagact accccaatga 20340 aaccatgtta cggttcatat gcaaaaccca caaatgaaaa tggagggcaa ggcattcttg 20400 taaagcaaca aaatggaaag ctagaaagtc aagtggaaat gcaatttttc tcaactactg 20460 aggcgaccgc aggcaatggt gataacttga ctcctaaagt ggtattgtac agtgaagatg 20520 tagatataga aaccccagac actcatattt cttacatgcc cactattaag gaaggtaact 20580 cacgagaact aatgggccaa caatctatgc ccaacaggcc taattacatt gcttttaggg 20640 acaattttat tggtctaatg tattacaaca gcacgggtaa tatgggtgtt ctggcgggcc 20700 aagcatcgca gttgaatgct gttgtagatt tgcaagacag aaacacagag ctttcatacc 20760 agcttttgct tgattccatt ggtgatagaa ccaggtactt ttctatgtgg aatcaggctg 20820 ttgacagcta tgatccagat gttagaatta ttgaaaatca tggaactgaa gatgaacttc 20880 caaattactg ctttccactg ggaggtgtga ttaatacaga gactcttacc aaggtaaaac 20940 ctaaaacagg tcaggaaaat ggatgggaaa aagatgctac agaattttca gataaaaatg 21000 aaataagagt tggaaataat tttgccatgg aaatcaatct aaatgccaac ctgtggagaa 21060 atttcctgta ctccaacata gcgctgtatt tgcccgacaa gctaaagtac agtccttcca 21120 acgtaaaaat ttctgataac ccaaacacct acgactacat gaacaagcga gtggtggctc 21180 ccgggttagt ggactgctac attaaccttg gagcacgctg gtcccttgac tatatggaca 21240 acgtcaaccc atttaaccac caccgcaatg ctggcctgcg ctaccgctca atgttgctgg 21300 gcaatggtcg ctatgtgccc ttccacatcc aggtgcctca gaagttcttt gccattaaaa 21360 acctccttct cctgccgggc tcatacacct acgagtggaa cttcaggaag gatgttaaca 21420 tggttctgca gagctcccta ggaaatgacc taagggttga cggagccagc attaagtttg 21480 atagcatttg cctttacgcc accttcttcc ccatggccca caacaccgcc tccacgcttg 21540 aggccatgct tagaaacgac accaacgacc agtcctttaa cgactatctc tccgccgcca 21600 acatgctcta ccctataccc gccaacgcta ccaacgtgcc catatccatc ccctcccgca 21660 actgggcggc tttccgcggc tgggccttca cgcgccttaa gactaaggaa accccatcac 21720 tgggctcggg ctacgaccct tattacacct actctggctc tataccctac ctagatggaa 21780 ccttttacct caaccacacc tttaagaagg tggccattac ctttgactct tctgtcagct 21840 ggcctggcaa tgaccgcctg cttaccccca acgagtttga aattaagcgc tcagttgacg 21900 gggagggtta caacgttgcc cagtgtaaca tgaccaaaga ctggttcctg gtacaaatgc 21960 tagctaacta caacattggc taccagggct tctatatccc agagagctac aaggaccgca 22020 tgtactcctt ctttagaaac ttccagccca tgagccgtca ggtggtggat gatactaaat 22080 acaaggacta ccaacaggtg ggcatcctac accaacacaa caactctgga tttgttggct 22140 accttgcccc caccatgcgc gaaggacagg cctaccctgc taacttcccc tatccgctta 22200 taggcaagac cgcagttgac agcattaccc agaaaaagtt tctttgcgat cgcacccttt 22260 ggcgcatccc attctccagt aactttatgt ccatgggcgc actcacagac ctgggccaaa 22320 accttctcta cgccaactcc gcccacgcgc tagacatgac ttttgaggtg gatcccatgg 22380 acgagcccac ccttctttat gttttgtttg aagtctttga cgtggtccgt gtgcaccggc 22440 cgcaccgcgg cgtcatcgaa accgtgtacc tgcgcacgcc cttctcggcc ggcaacgcca 22500 caacataaag aagcaagcaa catcaacaac agctgccgcc atgggctcca gtgagcagga 22560 actgaaagcc attgtcaaag atcttggttg tgggccatat tttttgggca cctatgacaa 22620 gcgctttcca ggctttgttt ctccacacaa gctcgcctgc gccatagtca atacggccgg 22680 tcgcgagact gggggcgtac actggatggc ctttgcctgg aacccgcact caaaaacatg 22740 ctacctcttt gagccctttg gcttttctga ccagcgactc aagcaggttt accagtttga 22800 gtacgagtca ctcctgcgcc gtagcgccat tgcttcttcc cccgaccgct gtataacgct 22860 ggaaaagtcc acccaaagcg tacaggggcc caactcggcc gcctgtggac tattctgctg 22920 catgtttctc cacgcctttg ccaactggcc ccaaactccc atggatcaca accccaccat 22980 gaaccttatt accggggtac ccaactccat gctcaacagt ccccaggtac agcccaccct 23040 gcgtcgcaac caggaacagc tctacagctt cctggagcgc cactcgccct acttccgcag 23100 ccacagtgcg cagattagga gcgccacttc tttttgtcac ttgaaaaaca tgtaaaaata 23160 atgtactaga gacactttca ataaaggcaa atgcttttat ttgtacactc tcgggtgatt 23220 atttaccccc acccttgccg tctgcgccgt ttaaaaatca aaggggttct gccgcgcatc 23280 gctatgcgcc actggcaggg acacgttgcg atactggtgt ttagtgctcc acttaaactc 23340 aggcacaacc atccgcggca gctcggtgaa gttttcactc cacaggctgc gcaccatcac 23400 caacgcgttt agcaggtcgg gcgccgatat cttgaagtcg cagttggggc ctccgccctg 23460 cgcgcgcgag ttgcgataca cagggttgca gcactggaac actatcagcg ccgggtggtg 23520 cacgctggcc agcacgctct tgtcggagat cagatccgcg tccaggtcct ccgcgttgct 23580 cagggcgaac ggagtcaact ttggtagctg ccttcccaaa aagggcgcgt gcccaggctt 23640 tgagttgcac tcgcaccgta gtggcatcaa aaggtgaccg tgcccggtct gggcgttagg 23700 atacagcgcc tgcataaaag ccttgatctg cttaaaagcc acctgagcct ttgcgccttc 23760 agagaagaac atgccgcaag acttgccgga aaactgattg gccggacagg ccgcgtcgtg 23820 cacgcagcac cttgcgtcgg tgttggagat ctgcaccaca tttcggcccc accggttctt 23880 cacgatcttg gccttgctag actgctcctt cagcgcgcgc tgcccgtttt cgctcgtcac 23940 atccatttca atcacgtgct ccttatttat cataatgctt ccgtgtagac acttaagctc 24000 gccttcgatc tcagcgcagc ggtgcagcca caacgcgcag cccgtgggct cgtgatgctt 24060 gtaggtcacc tctgcaaacg actgcaggta cgcctgcagg aatcgcccca tcatcgtcac 24120 aaaggtcttg ttgctggtga aggtcagctg caacccgcgg tgctcctcgt tcagccaggt 24180 cttgcatacg gccgccagag cttccacttg gtcaggcagt agtttgaagt tcgcctttag 24240 atcgttatcc acgtggtact tgtccatcag cgcgcgcgca gcctccatgc ccttctccca 24300 cgcagacacg atcggcacac tcagcgggtt catcaccgta atttcacttt ccgcttcgct 24360 gggctcttcc tcttcctctt gcgtccgcat accacgcgcc actgggtcgt cttcattcag 24420 ccgccgcact gtgcgcttac ctcctttgcc atgcttgatt agcaccggtg ggttgctgaa 24480 acccaccatt tgtagcgcca catcttctct ttcttcctcg ctgtccacga ttacctctgg 24540 tgatggcggg cgctcgggct tgggagaagg gcgcttcttt ttcttcttgg gcgcaatggc 24600 caaatccgcc gccgaggtcg atggccgcgg gctgggtgtg cgcggcacca gcgcgtcttg 24660 tgatgagtct tcctcgtcct cggactcgat acgccgcctc atccgctttt ttgggggcgc 24720 ccggggaggc ggcggcgacg gggacgggga cgacacgtcc tccatggttg ggggacgtcg 24780 cgccgcaccg cgtccgcgct cgggggtggt ttcgcgctgc tcctcttccc gactggccat 24840 ttccttctcc tataggcaga aaaagatcat ggagtcagtc gagaagaagg acagcctaac 24900 cgccccctct gagttcgcca ccaccgcctc caccgatgcc gccaacgcgc ctaccacctt 24960 ccccgtcgag gcacccccgc ttgaggagga ggaagtgatt atcgagcagg acccaggttt 25020 tgtaagcgaa gacgacgagg accgctcagt accaacagag gataaaaagc aagaccagga 25080 caacgcagag gcaaacgagg aacaagtcgg gcggggggac gaaaggcatg gcgactacct 25140 agatgtggga gacgacgtgc tgttgaagca tctgcagcgc cagtgcgcca ttatctgcga 25200 cgcgttgcaa gagcgcagcg atgtgcccct cgccatagcg gatgtcagcc ttgcctacga 25260 acgccaccta ttctcaccgc gcgtaccccc caaacgccaa gaaaacggca catgcgagcc 25320 caacccgcgc ctcaacttct accccgtatt tgccgtgcca gaggtgcttg ccacctatca 25380 catctttttc caaaactgca agatacccct atcctgccgt gccaaccgca gccgagcgga 25440 caagcagctg gccttgcggc agggcgctgt catacctgat atcgcctcgc tcaacgaagt 25500 gccaaaaatc tttgagggtc ttggacgcga cgagaagcgc gcggcaaacg ctctgcaaca 25560 ggaaaacagc gaaaatgaaa gtcactctgg agtgttggtg gaactcgagg gtgacaacgc 25620 gcgcctagcc gtactaaaac gcagcatcga ggtcacccac tttgcctacc cggcacttaa 25680 cctacccccc aaggtcatga gcacagtcat gagtgagctg atcgtgcgcc gtgcgcagcc 25740 cctggagagg gatgcaaatt tgcaagaaca aacagaggag ggcctacccg cagttggcga 25800 cgagcagcta gcgcgctggc ttcaaacgcg cgagcctgcc gacttggagg agcgacgcaa 25860 actaatgatg gccgcagtgc tcgttaccgt ggagcttgag tgcatgcagc ggttctttgc 25920 tgacccggag atgcagcgca agctagagga aacattgcac tacacctttc gacagggcta 25980 cgtacgccag gcctgcaaga tctccaacgt ggagctctgc aacctggtct cctaccttgg 26040 aattttgcac gaaaaccgcc ttgggcaaaa cgtgcttcat tccacgctca agggcgaggc 26100 gcgccgcgac tacgtccgcg actgcgttta cttatttcta tgctacacct ggcagacggc 26160 catgggcgtt tggcagcagt gcttggagga gtgcaacctc aaggagctgc agaaactgct 26220 aaagcaaaac ttgaaggacc tatggacggc cttcaacgag cgctccgtgg ccgcgcacct 26280 ggcggacatc attttccccg aacgcctgct taaaaccctg caacagggtc tgccagactt 26340 caccagtcaa agcatgttgc agaactttag gaactttatc ctagagcgct caggaatctt 26400 gcccgccacc tgctgtgcac ttcctagcga ctttgtgccc attaagtacc gcgaatgccc 26460 tccgccgctt tggggccact gctaccttct gcagctagcc aactaccttg cctaccactc 26520 tgacataatg gaagacgtga gcggtgacgg tctactggag tgtcactgtc gctgcaacct 26580 atgcaccccg caccgctccc tggtttgcaa ttcgcagctg cttaacgaaa gtcaaattat 26640 cggtaccttt gagctgcagg gtccctcgcc tgacgaaaag tccgcggctc cggggttgaa 26700 actcactccg gggctgtgga cgtcggctta ccttcgcaaa tttgtacctg aggactacca 26760 cgcccacgag attaggttct acgaagacca atcccgcccg ccaaatgcgg agcttaccgc 26820 ctgcgtcatt acccagggcc acattcttgg ccaattgcaa gccatcaaca aagcccgcca 26880 agagtttctg ctacgaaagg gacggggggt ttacttggac ccccagtccg gcgaggagct 26940 caacccaatc cccccgccgc cgcagcccta tcagcagcag ccgcgggccc ttgcttccca 27000 ggatggcacc caaaaagaag ctgcagctgc cgccgccacc cacggacgag gaggaatact 27060 gggacagtca ggcagaggag gttttggacg aggaggagga ggacatgatg gaagactggg 27120 agagcctaga cgaggaagct tccgaggtcg aagaggtgtc agacgaaaca ccgtcaccct 27180 cggtcgcatt cccctcgccg gcgccccaga aatcggcaac cggttccagc atggctacaa 27240 cctccgctcc tcaggcgccg ccggcactgc ccgttcgccg acccaaccgt agatgggaca 27300 ccactggaac cagggccggt aagtccaagc agccgccgcc gttagcccaa gagcaacaac 27360 agcgccaagg ctaccgctca tggcgcgggc acaagaacgc catagttgct tgcttgcaag 27420 actgtggggg caacatctcc ttcgcccgcc gctttcttct ctaccatcac ggcgtggcct 27480 tcccccgtaa catcctgcat tactaccgtc atctctacag cccatactgc accggcggca 27540 gcggcagcgg cagcaacagc agcggccaca cagaagcaaa ggcgaccgga tagcaagact 27600 ctgacaaagc ccaagaaatc cacagcggcg gcagcagcag gaggaggagc gctgcgtctg 27660 gcgcccaacg aacccgtatc gacccgcgag cttagaaaca ggatttttcc cactctgtat 27720 gctatatttc aacagagcag gggccaagaa caagagctga aaataaaaaa caggtctctg 27780 cgatccctca cccgcagctg cctgtatcac aaaagcgaag atcagcttcg gcgcacgctg 27840 gaagacgcgg aggctctctt cagtaaatac tgcgcgctga ctcttaagga ctagtttcgc 27900 gccctttctc aaatttaagc gcgaaaacta cgtcatctcc agcggccaca cccggcgcca 27960 gcacctgtcg tcagcgccat ttcaactttg tatacaaaag ttgtgatgag caaggaaatt 28020 cccacgccct acatgtggag ttaccagcca caaatgggac ttgcggctgg agctgcccaa 28080 gactactcaa cccgaataaa ctacatgagc gcgggacccc acatgatatc ccgggtcaac 28140 ggaatacgcg cccaccgaaa ccgaattctc ctggaacagg cggctattac caccacacct 28200 cgtaataacc ttaatccccg tagttggccc gctgccctgg tgtaccagga aagtcccgct 28260 cccaccactg tggtacttcc cagagacgcc caggccgaag ttcagatgac taactcaggg 28320 gcgcagcttg cgggcggctt tcgtcacagg gtgcggtcgc ccgggcaggg tataactcac 28380 ctgacaatca gagggcgagg tattcagctc aacgacgagt cggtgagctc ctcgcttggt 28440 ctccgtccgg acgggacatt tcagatcggc ggcgccggcc gctcttcatt cacgcctcgt 28500 caggcaatcc taactctgca gacctcgtcc tctgagccgc gctctggagg cattggaact 28560 ctgcaattta ttgaggagtt tgtgccatcg gtctacttta accccttctc gggacctccc 28620 ggccactatc cggatcaatt tattcctaac tttgacgcgg taaaggactc ggcggacggc 28680 tacgactgaa tgttaagtgg agaggcagag caactgcgcc tgaaacacct ggtccactgt 28740 cgccgccaca agtgctttgc ccgcgactcc ggtgagtttt gctactttga attgcccgag 28800 gatcatatcg agggcccggc gcacggcgtc cggcttaccg cccagggaga gcttgcccgt 28860 agcctgattc gggagtttac ccagcgcccc ctgctagttg agcgggacag gggaccctgt 28920 gttctcactg tgatttgcaa ctgtcctaac cctggattac atcaagatct ttgttgccat 28980 ctctgtgctg agtataataa atacagaaat taaaatatac tggggctcct atcgccatcc 29040 tgtaaacgcc accgtcttca cccgcccaag caaaccaagg cgaaccttac ctggtacttt 29100 taacatctct ccctctgtga tttacaacag tttcaaccca gacggagtga gtctacgaga 29160 gaacctctcc gagctcagct actccatcag aaaaaacacc accctcctta cctgccggga 29220 acgtacgagt gcgtcaccgg ccgctgcacc acacctaccg cctgaccgta aaccagactt 29280 tttccggaca gacctcaata actctgttta ccagaacagg aggtgagctt agaaaaccct 29340 tagggtatta ggccaaaggc gcagctactg tggggtttat gaacaattca agcaactcta 29400 cgggctattc taattcaggt ttctctagaa tcggggttgg ggttattctc tgtcttgtga 29460 ttctctttat tcttatacta acgcttctct gcctaaggct cgccgcctgc tgtgtgcaca 29520 tttgcattta ttgtcagctt tttaaacgct ggggtcgcca cccaagatga ttaggtacat 29580 aatcctaggt ttactcaccc ttgcgtcagc ccacggtacc acccaaaagg tggattttaa 29640 ggagccagcc tgtaatgtta cattcgcagc tgaagctaat gagtgcacca ctcttataaa 29700 atgcaccaca gaacatgaaa agctgcttat tcgccacaaa aacaaaattg gcaagtatgc 29760 tgtttatgct atttggcagc caggtgacac tacagagtat aatgttacag ttttccaggg 29820 taaaagtcat aaaactttta tgtatacttt tccattttat gaaatgtgcg acattaccat 29880 gtacatgagc aaacagtata agttgtggcc cccacaaaat tgtgtggaaa acactggcac 29940 tttctgctgc actgctatgc taattacagt gctcgctttg gtctgtaccc tactctatat 30000 taaatacaaa agcagacgca gctttattga ggaaaagaaa atgccttaat ttactaagtt 30060 acaaagctaa tgtcaccact aactgcttta ctcgctgctt gcaaaacaaa ttcaaaaagt 30120 tagcattata attagaatag gatttaaacc ccccggtcat ttcctgctca ataccattcc 30180 cctgaacaat tgactctatg tgggatatgc tccagcgcta caaccttgaa gtcaggcttc 30240 ctggatgtca gcatctgact ttggccagca cctgtcccgc ggatttgttc cagtccaact 30300 acagcgaccc accctaacag agatgaccaa cacaaccaac gcggccgccg ctaccggact 30360 tacatctacc acaaatacac cccaagtttc tgcctttgtc aataactggg ataacttggg 30420 catgtggtgg ttctccatag cgcttatgtt tgtatgcctt attattatgt ggctcatctg 30480 ctgcctaaag cgcaaacgcg cccgaccacc catctatagt cccatcattg tgctacaccc 30540 aaacaatgat ggaatccata gattggacgg actgaaacac atgttctttt ctcttacagt 30600 atgattaaat gagacatgat tcctcgagtt tttatattac tgacccttgt tgcgcttttt 30660 tgtgcgtgct ccacattggc tgcggtttct cacatcgaag tagactgcat tccagccttc 30720 acagtctatt tgctttacgg atttgtcacc ctcacgctca tctgcagcct catcactgtg 30780 gtcatcgcct ttatccagtg cattgactgg gtctgtgtgc gctttgcata tctcagacac 30840 catccccagt acagggacag gactatagct gagcttctta gaattcttta attatgaaat 30900 ttactgtgac ttttctgctg attatttgca ccctatctgc gttttgttcc ccgacctcca 30960 agcctcaaag acatatatca tgcagattca ctcgtatatg gaatattcca agttgctaca 31020 atgaaaaaag cgatctttcc gaagcctggt tatatgcaat catctctgtt atggtgttct 31080 gcagtaccat cttagcccta gctatatatc cctaccttga cattggctgg aacgcaatag 31140 atgccatgaa ccacccaact ttccccgcgc ccgctatgct tccactgcaa caagttgttg 31200 ccggcggctt tgtcccagcc aatcagcctc gcccaccttc tcccaccccc actgaaatca 31260 gctactttaa tctaacagga ggagatgact gacaccctag atctagaaat ggacggaatt 31320 attacagagc agcgcctgct agaaagacgc agggcagcgg ccgagcaaca gcgcatgaat 31380 caagagctcc aagacatggt taacttgcac cagtgcaaaa ggggtatctt ttgtctggta 31440 aagcaggcca aagtcaccta cgacagtaat accaccggac accgccttag ctacaagttg 31500 ccaaccaagc gtcagaaatt ggtggtcatg gtgggagaaa agcccattac cataactcag 31560 cactcggtag aaaccgaagg ctgcattcac tcaccttgtc aaggacctga ggatctctgc 31620 acccttatta agaccctgtg cggtctcaaa gatcttattc cctttaacta ataaaaaaaa 31680 ataataaagc atcacttact taaaatcagt tagcaaattt ctgtccagtt tattcagcag 31740 cacctccttg ccctcctccc agctctggta ttgcagcttc ctcctggctg caaactttct 31800 ccacaatcta aatggaatgt cagtttcctc ctgttcctgt ccatccgcac ccactatctt 31860 catgttgttg cagatgaagc gcgcaagacc gtctgaagat accttcaacc ccgtgtatcc 31920 atatgacacg gaaaccggtc ctccaactgt gccttttctt actcctccct ttgtatcccc 31980 caatgggttt caagagagtc cccctggggt actctctttg cgcctatccg aacctctagt 32040 tacctccaat ggcatgcttg cgctcaaaat gggcaacggc ctctctctgg acgaggccgg 32100 caaccttacc tcccaaaatg taaccactgt gagcccacct ctcaaaaaaa ccaagtcaaa 32160 cataaacctg gaaatatctg cacccctcac agttacctca gaagccctaa ctgtggctgc 32220 cgccgcacct ctaatggtcg cgggcaacac actcaccatg caatcacagg ccccgctaac 32280 cgtgcacgac tccaaactta gcattgccac ccaaggaccc ctcacagtgt cagaaggaaa 32340 gctagccctg caaacatcag gccccctcac caccaccgat agcagtaccc ttactatcac 32400 tgcctcaccc cctctaacta ctgccactgg tagcttgggc attgacttga aagagcccat 32460 ttatacacaa aatggaaaac taggactaaa gtacggggct cctttgcatg taacagacga 32520 cctaaacact ttgaccgtag caactggtcc aggtgtgact attaataata cttccttgca 32580 aactaaagtt actggagcct tgggttttga ttcacaaggc aatatgcaac ttaatgtagc 32640 aggaggacta aggattgatt ctcaaaacag acgccttata cttgatgtta gttatccgtt 32700 tgatgctcaa aaccaactaa atctaagact aggacagggc cctcttttta taaactcagc 32760 ccacaacttg gatattaact acaacaaagg cctttacttg tttacagctt caaacaattc 32820 caaaaagctt gaggttaacc taagcactgc caaggggttg atgtttgacg ctacagccat 32880 agccattaat gcaggagatg ggcttgaatt tggttcacct aatgcaccaa acacaaatcc 32940 cctcaaaaca aaaattggcc atggcctaga atttgattca aacaaggcta tggttcctaa 33000 actaggaact ggccttagtt ttgacagcac aggtgccatt acagtaggaa acaaaaataa 33060 tgataagcta actttgtgga ccacaccagc tccatctcct aactgtagac taaatgcaga 33120 gaaagatgct aaactcactt tggtcttaac aaaatgtggc agtcaaatac ttgctacagt 33180 ttcagttttg gctgttaaag gcagtttggc tccaatatct ggaacagttc aaagtgctca 33240 tcttattata agatttgacg aaaatggagt gctactaaac aattccttcc tggacccaga 33300 atattggaac tttagaaatg gagatcttac tgaaggcaca gcctatacaa acgctgttgg 33360 atttatgcct aacctatcag cttatccaaa atctcacggt aaaactgcca aaagtaacat 33420 tgtcagtcaa gtttacttaa acggagacaa aactaaacct gtaacactaa ccattacact 33480 aaacggtaca caggaaacag gagacacaac tccaagtgca tactctatgt cattttcatg 33540 ggactggtct ggccacaact acattaatga aatatttgcc acatcctctt acactttttc 33600 atacattgcc caagaataaa gaatcgtttg tgttatgttt caacgtgttt atttttcaat 33660 tgcagaaaat ttcaagtcat ttttcattca gtagtatagc cccaccacca catagcttat 33720 acagatcacc gtacctcaac tttgtataat aaagttgtaa tcaaactcac agaaccctag 33780 tattcaacct gccacctccc tcccaacaca cagagtacac agtcctttct ccccggctgg 33840 ccttaaaaag catcatatca tgggtaacag acatattctt aggtgttata ttccacacgg 33900 tttcctgtcg agccaaacgc tcatcagtga tattaataaa ctccccgggc agctcactta 33960 agttcatgtc gctgtccagc tgctgagcca caggctgctg tccaacttgc ggttgcttaa 34020 cgggcggcga aggagaagtc cacgcctaca tgggggtaga gtcataatcg tgcatcagga 34080 tagggcggtg gtgctgcagc agcgcgcgaa taaactgctg ccgccgccgc tccgtcctgc 34140 aggaatacaa catggcagtg gtctcctcag cgatgattcg caccgcccgc agcataaggc 34200 gccttgtcct ccgggcacag cagcgcaccc tgatctcact taaatcagca cagtaactgc 34260 agcacagcac cacaatattg ttcaaaatcc cacagtgcaa ggcgctgtat ccaaagctca 34320 tggcggggac cacagaaccc acgtggccat cataccacaa gcgcaggtag attaagtggc 34380 gacccctcat aaacacgctg gacataaaca ttacctcttt tggcatgttg taattcacca 34440 cctcccggta ccatataaac ctctgattaa acatggcgcc atccaccacc atcctaaacc 34500 agctggccaa aacctgcccg ccggctatac actgcaggga accgggactg gaacaatgac 34560 agtggagagc ccaggactcg taaccatgga tcatcatgct cgtcatgata tcaatgttgg 34620 cacaacacag gcacacgtgc atacacttcc tcaggattac aagctcctcc cgcgttagaa 34680 ccatatccca gggaacaacc cattcctgaa tcagcgtaaa tcccacactg cagggaagac 34740 ctcgcacgta actcacgttg tgcattgtca aagtgttaca ttcgggcagc agcggatgat 34800 cctccagtat ggtagcgcgg gtttctgtct caaaaggagg tagacgatcc ctactgtacg 34860 gagtgcgccg agacaaccga gatcgtgttg gtcgtagtgt catgccaaat ggaacgccgg 34920 acgtagtcat atttcctgaa gcaaaaccag gtgcgggcgt gacaaacaga tctgcgtctc 34980 cggtctcgcc gcttagatcg ctctgtgtag tagttgtagt atatccactc tctcaaagca 35040 tccaggcgcc ccctggcttc gggttctatg taaactcctt catgcgccgc tgccctgata 35100 acatccacca ccgcagaata agccacaccc agccaaccta cacattcgtt ctgcgagtca 35160 cacacgggag gagcgggaag agctggaaga accatgtttt tttttttatt ccaaaagatt 35220 atccaaaacc tcaaaatgaa gatctattaa gtgaacgcgc tcccctccgg tggcgtggtc 35280 aaactctaca gccaaagaac agataatggc atttgtaaga tgttgcacaa tggcttccaa 35340 aaggcaaacg gccctcacgt ccaagtggac gtaaaggcta aacccttcag ggtgaatctc 35400 ctctataaac attccagcac cttcaaccat gcccaaataa ttctcatctc gccaccttct 35460 caatatatct ctaagcaaat cccgaatatt aagtccggcc attgtaaaaa tctgctccag 35520 agcgccctcc accttcagcc tcaagcagcg aatcatgatt gcaaaaattc aggttcctca 35580 cagacctgta taagattcaa aagcggaaca ttaacaaaaa taccgcgatc ccgtaggtcc 35640 cttcgcaggg ccagctgaac ataatcgtgc aggtctgcac ggaccagcgc ggccacttcc 35700 ccgccaggaa ccatgacaaa agaacccaca ctgattatga cacgcatact cggagctatg 35760 ctaaccagcg tagccccgat gtaagcttgt tgcatgggcg gcgatataaa atgcaaggtg 35820 ctgctcaaaa aatcaggcaa agcctcgcgc aaaaaagaaa gcacatcgta gtcatgctca 35880 tgcagataaa ggcaggtaag ctccggaacc accacagaaa aagacaccat ttttctctca 35940 aacatgtctg cgggtttctg cataaacaca aaataaaata acaaaaaaac atttaaacat 36000 tagaagcctg tcttacaaca ggaaaaacaa cccttataag cataagacgg actacggcca 36060 tgccggcgtg accgtaaaaa aactggtcac cgtgattaaa aagcaccacc gacagctcct 36120 cggtcatgtc cggagtcata atgtaagact cggtaaacac atcaggttga ttcacatcgg 36180 tcagtgctaa aaagcgaccg aaatagcccg ggggaataca tacccgcagg cgtagagaca 36240 acattacagc ccccatagga ggtataacaa aattaatagg agagaaaaac acataaacac 36300 ctgaaaaacc ctcctgccta ggcaaaatag caccctcccg ctccagaaca acatacagcg 36360 cttccacagc ggcagccata acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa 36420 aacaccactc gacacggcac cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag 36480 agcgagtata tataggacta aaaaatgacg taacggttaa agtccacaaa aaacacccag 36540 aaaaccgcac gcgaacctac gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa 36600 tcgtcacttc cgttttccca cgttacgtca cttcccattt taagaaaact acaattccca 36660 acacatacaa gttactccgc cctaaaacct acgtcacccg ccccgttccc acgccccgcg 36720 ccacgtcaca aactccaccc cctcattatc atattggctt caatccaaaa taaggtatat 36780 tattgatgat g 36791 <210> 3 <211> 36791 <212> DNA <213> Artificial Sequence <220> <223> Synthetic adenovirus genome E1B-55k-P2A-YPet <400> 3 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420 cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480 tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540 tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600 aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660 tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720 cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780 gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840 cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900 ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960 cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020 caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080 ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140 tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200 gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260 ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320 cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380 ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440 gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500 cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560 ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620 gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680 cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740 ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800 tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860 gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920 caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980 gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040 agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100 aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160 cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220 gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280 gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340 gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400 gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460 tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520 ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580 tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640 agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700 tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760 gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820 acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880 gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940 aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000 ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060 gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120 tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180 acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240 aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300 tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360 gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420 tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480 ttggctctag cgatgaagat acagatggaa gcggagctac taacttcagc ctgctgaagc 3540 aggctggtga cgtcgaggag aatcctggcc caatggtgag caaaggcgaa gagctgttca 3600 ccggcgtggt gcccatcctg gtggagctgg acggcgacgt gaacggccac aagttcagcg 3660 tgagcggcga gggcgagggc gacgccacct acggcaagct gaccctgaag ctgctgtgca 3720 ccaccggcaa gctgcccgtg ccctggccca ccctggtgac caccctgggc tacggcgtgc 3780 agtgcttcgc ccggtacccc gaccacatga agcagcacga cttcttcaag agcgccatgc 3840 ccgagggcta cgtgcaggag cggaccatct tcttcaagga cgacggcaac tacaagaccc 3900 gggccgaggt gaagttcgag ggcgacaccc tggtgaaccg gatcgagctg aagggcatcg 3960 acttcaagga ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca 4020 acgtgtacat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccggc 4080 acaacatcga ggacggcggc gtgcagctgg ccgaccacta ccagcagaac acccccatcg 4140 gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagagc gccctgttca 4200 aggaccccaa cgagaagcgg gaccacatgg tgctgctgga gttcctgacc gccgccggca 4260 tcaccgaggg catgaacgag ctctataagt gaggtactga aatgtgtggg cgtggcttaa 4320 gggtgggaaa gaatatataa ggtgggggtc ttatgtagtt ttgtatctgt tttgcagcag 4380 ccgccgccgc ccaacttttc tatacaaagt tgtaatgagc accaactcgt ttgatggaag 4440 cattgtgagc tcatatttga caacgcgcat gcccccatgg gccggggtgc gtcagaatgt 4500 gatgggctcc agcattgatg gtcgccccgt cctgcccgca aactctacta ccttgaccta 4560 cgagaccgtg tctggaacgc cgttggagac tgcagcctcc gccgccgctt cagccgctgc 4620 agccaccgcc cgcgggattg tgactgactt tgctttcctg agcccgcttg caagcagtgc 4680 agcttcccgt tcatccgccc gcgatgacaa gttgacggct cttttggcac aattggattc 4740 tttgacccgg gaacttaatg tcgtttctca gcagctgttg gatctgcgcc agcaggtttc 4800 tgccctgaag gcttcctccc ctcccaatgc ggtttaaaac ataaataaaa aaccagactc 4860 tgtttggatt tggatcaagc ataagtgtct tgctgtcttt atttaggggt tttgcgcgcg 4920 cggtaggccc gggaccagcg gtctcggtcg ttgagggtcc tgtgtatttt ttccaggacg 4980 tggtaaaggt gactctggat gttcagatac atgggcataa gcccgtctct ggggtggagg 5040 tagcaccact gcagagcttc atgctgcggg gtggtgttgt agatgatcca gtcgtagcag 5100 gagcgctggg cgtggtgcct aaaaatgtct ttcagtagca agctgattgc caggggcagg 5160 cccttggtgt aagtgtttac aaagcggtta agctgggatg ggtgcatacg tggggatatg 5220 agatgcatct tggactgtat ttttaggttg gctatgttcc cagccatatc cctccgggga 5280 ttcatgttgt gcagaaccac cagcacagtg tatccggtgc acttgggaaa tttgtcatgt 5340 agcttagaag gaaatgcgtg gaagaacttg gagacgccct tgtgacctcc aagattttcc 5400 atgcattcgt ccataatgat ggcaatgggc ccacgggcgg cggcctgggc gaagatattt 5460 ctgggatcac taacgtcata gttgtgttcc aggatgagat cgtcataggc catttttaca 5520 aagcgcgggc ggagggtgcc agactgcggt ataatggttc catccggccc aggggcgtag 5580 ttaccctcac agatttgcat ttcccacgct ttgagttcag atggggggat catgtctacc 5640 tgcggggcga tgaagaaaac ggtttccggg gtaggggaga tcagctggga agaaagcagg 5700 ttcctgagca gctgcgactt accgcagccg gtgggcccgt aaatcacacc tattaccggg 5760 tgcaactggt agttaagaga gctgcagctg ccgtcatccc tgagcagggg ggccacttcg 5820 ttaagcatgt ccctgactcg catgttttcc ctgaccaaat ccgccagaag gcgctcgccg 5880 cccagcgata gcagttcttg caaggaagca aagtttttca acggtttgag accgtccgcc 5940 gtaggcatgc ttttgagcgt ttgaccaagc agttccaggc ggtcccacag ctcggtcacc 6000 tgctctacgg catctcgatc cagcatatct cctcgtttcg cgggttgggg cggctttcgc 6060 tgtacggcag tagtcggtgc tcgtccagac gggccagggt catgtctttc cacgggcgca 6120 gggtcctcgt cagcgtagtc tgggtcacgg tgaaggggtg cgctccgggc tgcgcgctgg 6180 ccagggtgcg cttgaggctg gtcctgctgg tgctgaagcg ctgccggtct tcgccctgcg 6240 cgtcggccag gtagcatttg accatggtgt catagtccag cccctccgcg gcgtggccct 6300 tggcgcgcag cttgcccttg gaggaggcgc cgcacgaggg gcagtgcaga cttttgaggg 6360 cgtagagctt gggcgcgaga aataccgatt ccggggagta ggcatccgcg ccgcaggccc 6420 cgcagacggt ctcgcattcc acgagccagg tgagctctgg ccgttcgggg tcaaaaacca 6480 ggtttccccc atgctttttg atgcgtttct tacctctggt ttccatgagc cggtgtccac 6540 gctcggtgac gaaaaggctg tccgtgtccc cgtatacaga cttgagaggc ctgtcctcga 6600 gcggtgttcc gcggtcctcc tcgtatagaa actcggacca ctctgagaca aaggctcgcg 6660 tccaggccag cacgaaggag gctaagtggg aggggtagcg gtcgttgtcc actagggggt 6720 ccactcgctc cagggtgtga agacacatgt cgccctcttc ggcatcaagg aaggtgattg 6780 gtttgtaggt gtaggccacg tgaccgggtg ttcctgaagg ggggctataa aagggggtgg 6840 gggcgcgttc gtcctcactc tcttccgcat cgctgtctgc gagggccagc tgttggggtg 6900 agtactccct ctgaaaagcg ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg 6960 aggaggattt gatattcacc tggcccgcgg tgatgccttt gagggtggcc gcatccatct 7020 ggtcagaaaa gacaatcttt ttgttgtcaa gcttggtggc aaacgacccg tagagggcgt 7080 tggacagcaa cttggcgatg gagcgcaggg tttggttttt gtcgcgatcg gcgcgctcct 7140 tggccgcgat gtttagctgc acgtattcgc gcgcaacgca ccgccattcg ggaaagacgg 7200 tggtgcgctc gtcgggcacc aggtgcacgc gccaaccgcg gttgtgcagg gtgacaaggt 7260 caacgctggt ggctacctct ccgcgtaggc gctcgttggt ccagcagagg cggccgccct 7320 tgcgcgagca gaatggcggt agggggtcta gctgcgtctc gtccgggggg tctgcgtcca 7380 cggtaaagac cccgggcagc aggcgcgcgt cgaagtagtc tatcttgcat ccttgcaagt 7440 ctagcgcctg ctgccatgcg cgggcggcaa gcgcgcgctc gtatgggttg agtgggggac 7500 cccatggcat ggggtgggtg agcgcggagg cgtacatgcc gcaaatgtcg taaacgtaga 7560 ggggctctct gagtattcca agatatgtag ggtagcatct tccaccgcgg atgctggcgc 7620 gcacgtaatc gtatagttcg tgcgagggag cgaggaggtc gggaccgagg ttgctacggg 7680 cgggctgctc tgctcggaag actatctgcc tgaagatggc atgtgagttg gatgatatgg 7740 ttggacgctg gaagacgttg aagctggcgt ctgtgagacc taccgcgtca cgcacgaagg 7800 aggcgtagga gtcgcgcagc ttgttgacca gctcggcggt gacctgcacg tctagggcgc 7860 agtagtccag ggtttccttg atgatgtcat acttatcctg tccctttttt ttccacagct 7920 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg 7980 cctccgaacg gtaagagcct agcatgtaga actggttgac ggcctggtag gcgcagcatc 8040 ccttttctac gggtagcgcg tatgcctgcg cggccttccg gagcgaggtg tgggtgagcg 8100 caaaggtgtc cctgaccatg actttgaggt actggtattt gaagtcagtg tcgtcgcatc 8160 cgccctgctc ccagagcaaa aagtccgtgc gctttttgga acgcggattt ggcagggcga 8220 aggtgacatc gttgaagagt atctttcccg cgcgaggcat aaagttgcgt gtgatgcgga 8280 agggtcccgg cacctcggaa cggttgttaa ttacctgggc ggcgagcacg atctcgtcaa 8340 agccgttgat gttgtggccc acaatgtaaa gttccaagaa gcgcgggatg cccttgatgg 8400 aaggcaattt tttaagttcc tcgtaggtga gctcttcagg ggagctgagc ccgtgctctg 8460 aaagggccca gtctgcaaga tgagggttgg aagcgacgaa tgagctccac aggtcacggg 8520 ccattagcat ttgcaggtgg tcgcgaaagg tcctaaactg gcgacctatg gccatttttt 8580 ctggggtgat gcagtagaag gtaagcgggt cttgttccca gcggtcccat ccaaggttcg 8640 cggctaggtc tcgcgcggca gtcactagag gctcatctcc gccgaacttc atgaccagca 8700 tgaagggcac gagctgcttc ccaaaggccc ccatccaagt ataggtctct acatcgtagg 8760 tgacaaagag acgctcggtg cgaggatgcg agccgatcgg gaagaactgg atctcccgcc 8820 accaattgga ggagtggcta ttgatgtggt gaaagtagaa gtccctgcga cgggccgaac 8880 actcgtgctg gcttttgtaa aaacgtgcgc agtactggca gcggtgcacg ggctgtacat 8940 cctgcacgag gttgacctga cgaccgcgca caaggaagca gagtgggaat ttgagcccct 9000 cgcctggcgg gtttggctgg tggtcttcta cttcggctgc ttgtccttga ccgtctggct 9060 gctcgagggg agttacggtg gatcggacca ccacgccgcg cgagcccaaa gtccagatgt 9120 ccgcgcgcgg cggtcggagc ttgatgacaa catcgcgcag atgggagctg tccatggtct 9180 ggagctcccg cggcgtcagg tcaggcggga gctcctgcag gtttacctcg catagacggg 9240 tcagggcgcg ggctagatcc aggtgatacc taatttccag gggctggttg gtggcggcgt 9300 cgatggcttg caagaggccg catccccgcg gcgcgactac ggtaccgcgc ggcgggcggt 9360 gggccgcggg ggtgtccttg gatgatgcat ctaaaagcgg tgacgcgggc gagcccccgg 9420 aggtaggggg ggctccggac ccgccgggag agggggcagg ggcacgtcgg cgccgcgcgc 9480 gggcaggagc tggtgctgcg cgcgtaggtt gctggcgaac gcgacgacgc ggcggttgat 9540 ctcctgaatc tggcgcctct gcgtgaagac gacgggcccg gtgagcttga gcctgaaaga 9600 gagttcgaca gaatcaattt cggtgtcgtt gacggcggcc tggcgcaaaa tctcctgcac 9660 gtctcctgag ttgtcttgat aggcgatctc ggccatgaac tgctcgatct cttcctcctg 9720 gagatctccg cgtccggctc gctccacggt ggcggcgagg tcgttggaaa tgcgggccat 9780 gagctgcgag aaggcgttga ggcctccctc gttccagacg cggctgtaga ccacgccccc 9840 ttcggcatcg cgggcgcgca tgaccacctg cgcgagattg agctccacgt gccgggcgaa 9900 gacggcgtag tttcgcaggc gctgaaagag gtagttgagg gtggtggcgg tgtgttctgc 9960 cacgaagaag tacataaccc agcgtcgcaa cgtggattcg ttgatatccc ccaaggcctc 10020 aaggcgctcc atggcctcgt agaagtccac ggcgaagttg aaaaactggg agttgcgcgc 10080 cgacacggtt aactcctcct ccagaagacg gatgagctcg gcgacagtgt cgcgcacctc 10140 gcgctcaaag gctacagggg cctcttcttc ttcttcaatc tcctcttcca taagggcctc 10200 cccttcttct tcttctggcg gcggtggggg aggggggaca cggcggcgac gacggcgcac 10260 cgggaggcgg tcgacaaagc gctcgatcat ctccccgcgg cgacggcgca tggtctcggt 10320 gacggcgcgg ccgttctcgc gggggcgcag ttggaagacg ccgcccgtca tgtcccggtt 10380 atgggttggc ggggggctgc catgcggcag ggatacggcg ctaacgatgc atctcaacaa 10440 ttgttgtgta ggtactccgc cgccgaggga cctgagcgag tccgcatcga ccggatcgga 10500 aaacctctcg agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc 10560 gggcggcagc gggcggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt 10620 aaagtaggcg gtcttgagac ggcggatggt cgacagaagc accatgtcct tgggtccggc 10680 ctgctgaatg cgcaggcggt cggccatgcc ccaggcttcg ttttgacatc ggcgcaggtc 10740 tttgtagtag tcttgcatga gcctttctac cggcacttct tcttctcctt cctcttgtcc 10800 tgcatctctt gcatctatcg ctgcggcggc ggcggagttt ggccgtaggt ggcgccctct 10860 tcctcccatg cgtgtgaccc cgaagcccct catcggctga agcagggcta ggtcggcgac 10920 aacgcgctcg gctaatatgg cctgctgcac ctgcgtgagg gtagactgga agtcatccat 10980 gtccacaaag cggtggtatg cgcccgtgtt gatggtgtaa gtgcagttgg ccataacgga 11040 ccagttaacg gtctggtgac ccggctgcga gagctcggtg tacctgagac gcgagtaagc 11100 cctcgagtca aatacgtagt cgttgcaagt ccgcaccagg tactggtatc ccaccaaaaa 11160 gtgcggcggc ggctggcggt agaggggcca gcgtagggtg gccggggctc cgggggcgag 11220 atcttccaac ataaggcgat gatatccgta gatgtacctg gacatccagg tgatgccggc 11280 ggcggtggtg gaggcgcgcg gaaagtcgcg gacgcggttc cagatgttgc gcagcggcaa 11340 aaagtgctcc atggtcggga cgctctggcc ggtcaggcgc gcgcaatcgt tgacgctcta 11400 gaccgtgcaa aaggagagcc tgtaagcggg cactcttccg tggtctggtg gataaattcg 11460 caagggtatc atggcggacg accggggttc gagccccgta tccggccgtc cgccgtgatc 11520 catgcggtta ccgcccgcgt gtcgaaccca ggtgtgcgac gtcagacaac gggggagtgc 11580 tccttttggc ttccttccag gcgcggcggc tgctgcgcta gcttttttgg ccactggccg 11640 cgcgcagcgt aagcggttag gctggaaagc gaaagcatta agtggctcgc tccctgtagc 11700 cggagggtta ttttccaagg gttgagtcgc gggacccccg gttcgagtct cggaccggcc 11760 ggactgcggc gaacgggggt ttgcctcccc gtcatgcaag accccgcttg caaattcctc 11820 cggaaacagg gacgagcccc ttttttgctt ttcccagatg catccggtgc tgcggcagat 11880 gcgcccccct cctcagcagc ggcaagagca agagcagcgg cagacatgca gggcaccctc 11940 ccctcctcct accgcgtcag gaggggcgac atccgcggtt gacgcggcag cagatggtga 12000 ttacgaaccc ccgcggcgcc gggcccggca ctacctggac ttggaggagg gcgagggcct 12060 ggcgcggcta ggagcgccct ctcctgagcg gtacccaagg gtgcagctga agcgtgatac 12120 gcgtgaggcg tacgtgccgc ggcagaacct gtttcgcgac cgcgagggag aggagcccga 12180 ggagatgcgg gatcgaaagt tccacgcagg gcgcgagctg cggcatggcc tgaatcgcga 12240 gcggttgctg cgcgaggagg actttgagcc cgacgcgcga accgggatta gtcccgcgcg 12300 cgcacacgtg gcggccgccg acctggtaac cgcatacgag cagacggtga accaggagat 12360 taactttcaa aaaagcttta acaaccacgt gcgtacgctt gtggcgcgcg aggaggtggc 12420 tataggactg atgcatctgt gggactttgt aagcgcgctg gagcaaaacc caaatagcaa 12480 gccgctcatg gcgcagctgt tccttatagt gcagcacagc agggacaacg aggcattcag 12540 ggatgcgctg ctaaacatag tagagcccga gggccgctgg ctgctcgatt tgataaacat 12600 cctgcagagc atagtggtgc aggagcgcag cttgagcctg gctgacaagg tggccgccat 12660 caactattcc atgcttagcc tgggcaagtt ttacgcccgc aagatatacc atacccctta 12720 cgttcccata gacaaggagg taaagatcga ggggttctac atgcgcatgg cgctgaaggt 12780 gcttaccttg agcgacgacc tgggcgttta tcgcaacgag cgcatccaca aggccgtgag 12840 cgtgagccgg cggcgcgagc tcagcgaccg cgagctgatg cacagcctgc aaagggccct 12900 ggctggcacg ggcagcggcg atagagaggc cgagtcctac tttgacgcgg gcgctgacct 12960 gcgctgggcc ccaagccgac gcgccctgga ggcagctggg gccggacctg ggctggcggt 13020 ggcacccgcg cgcgctggca acgtcggcgg cgtggaggaa tatgacgagg acgatgagta 13080 cgagccagag gacggcgagt actaagcggt gatgtttctg atcagatgat gcaagacgca 13140 acggacccgg cggtgcgggc ggcgctgcag agccagccgt ccggccttaa ctccacggac 13200 gactggcgcc aggtcatgga ccgcatcatg tcgctgactg cgcgcaatcc tgacgcgttc 13260 cggcagcagc cgcaggccaa ccggctctcc gcaattctgg aagcggtggt cccggcgcgc 13320 gcaaacccca cgcacgagaa ggtgctggcg atcgtaaacg cgctggccga aaacagggcc 13380 atccggcccg acgaggccgg cctggtctac gacgcgctgc ttcagcgcgt ggctcgttac 13440 aacagcggca acgtgcagac caacctggac cggctggtgg gggatgtgcg cgaggccgtg 13500 gcgcagcgtg agcgcgcgca gcagcagggc aacctgggct ccatggttgc actaaacgcc 13560 ttcctgagta cacagcccgc caacgtgccg cggggacagg aggactacac caactttgtg 13620 agcgcactgc ggctaatggt gactgagaca ccgcaaagtg aggtgtacca gtctgggcca 13680 gactattttt tccagaccag tagacaaggc ctgcagaccg taaacctgag ccaggctttc 13740 aaaaacttgc aggggctgtg gggggtgcgg gctcccacag gcgaccgcgc gaccgtgtct 13800 agcttgctga cgcccaactc gcgcctgttg ctgctgctaa tagcgccctt cacggacagt 13860 ggcagcgtgt cccgggacac atacctaggt cacttgctga cactgtaccg cgaggccata 13920 ggtcaggcgc atgtggacga gcatactttc caggagatta caagtgtcag ccgcgcgctg 13980 gggcaggagg acacgggcag cctggaggca accctaaact acctgctgac caaccggcgg 14040 cagaagatcc cctcgttgca cagtttaaac agcgaggagg agcgcatttt gcgctacgtg 14100 cagcagagcg tgagccttaa cctgatgcgc gacggggtaa cgcccagcgt ggcgctggac 14160 atgaccgcgc gcaacatgga accgggcatg tatgcctcaa accggccgtt tatcaaccgc 14220 ctaatggact acttgcatcg cgcggccgcc gtgaaccccg agtatttcac caatgccatc 14280 ttgaacccgc actggctacc gccccctggt ttctacaccg ggggattcga ggtgcccgag 14340 ggtaacgatg gattcctctg ggacgacata gacgacagcg tgttttcccc gcaaccgcag 14400 accctgctag agttgcaaca gcgcgagcag gcagaggcgg cgctgcgaaa ggaaagcttc 14460 cgcaggccaa gcagcttgtc cgatctaggc gctgcggccc cgcggtcaga tgctagtagc 14520 ccatttccaa gcttgatagg gtctcttacc agcactcgca ccacccgccc gcgcctgctg 14580 ggcgaggagg agtacctaaa caactcgctg ctgcagccgc agcgcgaaaa aaacctgcct 14640 ccggcatttc ccaacaacgg gatagagagc ctagtggaca agatgagtag atggaagacg 14700 tacgcgcagg agcacaggga cgtgccaggc ccgcgcccgc ccacccgtcg tcaaaggcac 14760 gaccgtcagc ggggtctggt gtgggaggac gatgactcgg cagacgacag cagcgtcctg 14820 gatttgggag ggagtggcaa cccgtttgcg caccttcgcc ccaggctggg gagaatgttt 14880 taaaaaaaaa aaagcatgat gcaaaataaa aaactcacca aggccatggc accgagcgtt 14940 ggttttcttg tattcccctt agtatgcggc gcgcggcgat gtatgaggaa ggtcctcctc 15000 cctcctacga gagtgtggtg agcgcggcgc cagtggcggc ggcgctgggt tctcccttcg 15060 atgctcccct ggacccgccg tttgtgcctc cgcggtacct gcggcctacc ggggggagaa 15120 acagcatccg ttactctgag ttggcacccc tattcgacac cacccgtgtg tacctggtgg 15180 acaacaagtc aacggatgtg gcatccctga actaccagaa cgaccacagc aactttctga 15240 ccacggtcat tcaaaacaat gactacagcc cgggggaggc aagcacacag accatcaatc 15300 ttgacgaccg gtcgcactgg ggcggcgacc tgaaaaccat cctgcatacc aacatgccaa 15360 atgtgaacga gttcatgttt accaataagt ttaaggcgcg ggtgatggtg tcgcgcttgc 15420 ctactaagga caatcaggtg gagctgaaat acgagtgggt ggagttcacg ctgcccgagg 15480 gcaactactc cgagaccatg accatagacc ttatgaacaa cgcgatcgtg gagcactact 15540 tgaaagtggg cagacagaac ggggttctgg aaagcgacat cggggtaaag tttgacaccc 15600 gcaacttcag actggggttt gaccccgtca ctggtcttgt catgcctggg gtatatacaa 15660 acgaagcctt ccatccagac atcattttgc tgccaggatg cggggtggac ttcacccaca 15720 gccgcctgag caacttgttg ggcatccgca agcggcaacc cttccaggag ggctttagga 15780 tcacctacga tgatctggag ggtggtaaca ttcccgcact gttggatgtg gacgcctacc 15840 aggcgagctt gaaagatgac accgaacagg gcgggggtgg cgcaggcggc agcaacagca 15900 gtggcagcgg cgcggaagag aactccaacg cggcagccgc ggcaatgcag ccggtggagg 15960 acatgaacga tcatgccatt cgcggcgaca cctttgccac acgggctgag gagaagcgcg 16020 ctgaggccga agcagcggcc gaagctgccg cccccgctgc gcaacccgag gtcgagaagc 16080 ctcagaagaa accggtgatc aaacccctga cagaggacag caagaaacgc agttacaacc 16140 taataagcaa tgacagcacc ttcacccagt accgcagctg gtaccttgca tacaactacg 16200 gcgaccctca gaccggaatc cgctcatgga ccctgctttg cactcctgac gtaacctgcg 16260 gctcggagca ggtctactgg tcgttgccag acatgatgca agaccccgtg accttccgct 16320 ccacgcgcca gatcagcaac tttccggtgg tgggcgccga gctgttgccc gtgcactcca 16380 agagcttcta caacgaccag gccgtctact cccaactcat ccgccagttt acctctctga 16440 cccacgtgtt caatcgcttt cccgagaacc agattttggc gcgcccgcca gcccccacca 16500 tcaccaccgt cagtgaaaac gttcctgctc tcacagatca cgggacgcta ccgctgcgca 16560 acagcatcgg aggagtccag cgagtgacca ttactgacgc cagacgccgc acctgcccct 16620 acgtttacaa ggccctgggc atagtctcgc cgcgcgtcct atcgagccgc actttttgag 16680 caagcatgtc catccttata tcgcccagca ataacacagg ctggggcctg cgcttcccaa 16740 gcaagatgtt tggcggggcc aagaagcgct ccgaccaaca cccagtgcgc gtgcgcgggc 16800 actaccgcgc gccctggggc gcgcacaaac gcggccgcac tgggcgcacc accgtcgatg 16860 acgccatcga cgcggtggtg gaggaggcgc gcaactacac gcccacgccg ccaccagtgt 16920 ccacagtgga cgcggccatt cagaccgtgg tgcgcggagc ccggcgctat gctaaaatga 16980 agagacggcg gaggcgcgta gcacgtcgcc accgccgccg acccggcact gccgcccaac 17040 gcgcggcggc ggccctgctt aaccgcgcac gtcgcaccgg ccgacgggcg gccatgcggg 17100 ccgctcgaag gctggccgcg ggtattgtca ctgtgccccc caggtccagg cgacgagcgg 17160 ccgccgcagc agccgcggcc attagtgcta tgactcaggg tcgcaggggc aacgtgtatt 17220 gggtgcgcga ctcggttagc ggcctgcgcg tgcccgtgcg cacccgcccc ccgcgcaact 17280 agattgcaag aaaaaactac ttagactcgt actgttgtat gtatccagcg gcggcggcgc 17340 gcaacgaagc tatgtccaag cgcaaaatca aagaagagat gctccaggtc atcgcgccgg 17400 agatctatgg ccccccgaag aaggaagagc aggattacaa gccccgaaag ctaaagcggg 17460 tcaaaaagaa aaagaaagat gatgatgatg aacttgacga cgaggtggaa ctgctgcacg 17520 ctaccgcgcc caggcgacgg gtacagtgga aaggtcgacg cgtaaaacgt gttttgcgac 17580 ccggcaccac cgtagtcttt acgcccggtg agcgctccac ccgcacctac aagcgcgtgt 17640 atgatgaggt gtacggcgac gaggacctgc ttgagcaggc caacgagcgc ctcggggagt 17700 ttgcctacgg aaagcggcat aaggacatgc tggcgttgcc gctggacgag ggcaacccaa 17760 cacctagcct aaagcccgta acactgcagc aggtgctgcc cgcgcttgca ccgtccgaag 17820 aaaagcgcgg cctaaagcgc gagtctggtg acttggcacc caccgtgcag ctgatggtac 17880 ccaagcgcca gcgactggaa gatgtcttgg aaaaaatgac cgtggaacct gggctggagc 17940 ccgaggtccg cgtgcggcca atcaagcagg tggcgccggg actgggcgtg cagaccgtgg 18000 acgttcagat acccactacc agtagcacca gtattgccac cgccacagag ggcatggaga 18060 cacaaacgtc cccggttgcc tcagcggtgg cggatgccgc ggtgcaggcg gtcgctgcgg 18120 ccgcgtccaa gacctctacg gaggtgcaaa cggacccgtg gatgtttcgc gtttcagccc 18180 cccggcgccc gcgcggttcg aggaagtacg gcgccgccag cgcgctactg cccgaatatg 18240 ccctacatcc ttccattgcg cctacccccg gctatcgtgg ctacacctac cgccccagaa 18300 gacgagcaac tacccgacgc cgaaccacca ctggaacccg ccgccgccgt cgccgtcgcc 18360 agcccgtgct ggccccgatt tccgtgcgca gggtggctcg cgaaggaggc aggaccctgg 18420 tgctgccaac agcgcgctac caccccagca tcgtttaaaa gccggtcttt gtggttcttg 18480 cagatatggc cctcacctgc cgcctccgtt tcccggtgcc gggattccga ggaagaatgc 18540 accgtaggag gggcatggcc ggccacggcc tgacgggcgg catgcgtcgt gcgcaccacc 18600 ggcggcggcg cgcgtcgcac cgtcgcatgc gcggcggtat cctgcccctc cttattccac 18660 tgatcgccgc ggcgattggc gccgtgcccg gaattgcatc cgtggccttg caggcgcaga 18720 gacactgatt aaaaacaagt tgcatgtgga aaaatcaaaa taaaaagtct ggactctcac 18780 gctcgcttgg tcctgtaact attttgtaga atggaagaca tcaactttgc gtctctggcc 18840 ccgcgacacg gctcgcgccc gttcatggga aactggcaag atatcggcac cagcaatatg 18900 agcggtggcg ccttcagctg gggctcgctg tggagcggca ttaaaaattt cggttccacc 18960 gttaagaact atggcagcaa ggcctggaac agcagcacag gccagatgct gagggataag 19020 ttgaaagagc aaaatttcca acaaaaggtg gtagatggcc tggcctctgg cattagcggg 19080 gtggtggacc tggccaacca ggcagtgcaa aataagatta acagtaagct tgatccccgc 19140 cctcccgtag aggagcctcc accggccgtg gagacagtgt ctccagaggg gcgtggcgaa 19200 aagcgtccgc gccccgacag ggaagaaact ctggtgacgc aaatagacga gcctccctcg 19260 tacgaggagg cactaaagca aggcctgccc accacccgtc ccatcgcgcc catggctacc 19320 ggagtgctgg gccagcacac acccgtaacg ctggacctgc ctccccccgc cgacacccag 19380 cagaaacctg tgctgccagg cccgaccgcc gttgttgtaa cccgtcctag ccgcgcgtcc 19440 ctgcgccgcg ccgccagcgg tccgcgatcg ttgcggcccg tagccagtgg caactggcaa 19500 agcacactga acagcatcgt gggtctgggg gtgcaatccc tgaagcgccg acgatgcttc 19560 tgaatagcta acgtgtcgta tgtgtgtcat gtatgcgtcc atgtcgccgc cagaggagct 19620 gctgagccgc cgcgcgcccg ctttccaaga tggctacccc ttcgatgatg ccgcagtggt 19680 cttacatgca catctcgggc caggacgcct cggagtacct gagccccggg ctggtgcagt 19740 ttgcccgcgc caccgagacg tacttcagcc tgaataacaa gtttagaaac cccacggtgg 19800 cgcctacgca cgacgtgacc acagaccggt cccagcgttt gacgctgcgg ttcatccctg 19860 tggaccgtga ggatactgcg tactcgtaca aggcgcggtt caccctagct gtgggtgata 19920 accgtgtgct ggacatggct tccacgtact ttgacatccg cggcgtgctg gacaggggcc 19980 ctacttttaa gccctactct ggcactgcct acaacgccct ggctcccaag ggtgccccaa 20040 atccttgcga atgggatgaa gctgctactg ctcttgaaat aaacctagaa gaagaggacg 20100 atgacaacga agacgaagta gacgagcaag ctgagcagca aaaaactcac gtatttgggc 20160 aggcgcctta ttctggtata aatattacaa aggagggtat tcaaataggt gtcgaaggtc 20220 aaacacctaa atatgccgat aaaacatttc aacctgaacc tcaaatagga gaatctcagt 20280 ggtacgaaac tgaaattaat catgcagctg ggagagtcct taaaaagact accccaatga 20340 aaccatgtta cggttcatat gcaaaaccca caaatgaaaa tggagggcaa ggcattcttg 20400 taaagcaaca aaatggaaag ctagaaagtc aagtggaaat gcaatttttc tcaactactg 20460 aggcgaccgc aggcaatggt gataacttga ctcctaaagt ggtattgtac agtgaagatg 20520 tagatataga aaccccagac actcatattt cttacatgcc cactattaag gaaggtaact 20580 cacgagaact aatgggccaa caatctatgc ccaacaggcc taattacatt gcttttaggg 20640 acaattttat tggtctaatg tattacaaca gcacgggtaa tatgggtgtt ctggcgggcc 20700 aagcatcgca gttgaatgct gttgtagatt tgcaagacag aaacacagag ctttcatacc 20760 agcttttgct tgattccatt ggtgatagaa ccaggtactt ttctatgtgg aatcaggctg 20820 ttgacagcta tgatccagat gttagaatta ttgaaaatca tggaactgaa gatgaacttc 20880 caaattactg ctttccactg ggaggtgtga ttaatacaga gactcttacc aaggtaaaac 20940 ctaaaacagg tcaggaaaat ggatgggaaa aagatgctac agaattttca gataaaaatg 21000 aaataagagt tggaaataat tttgccatgg aaatcaatct aaatgccaac ctgtggagaa 21060 atttcctgta ctccaacata gcgctgtatt tgcccgacaa gctaaagtac agtccttcca 21120 acgtaaaaat ttctgataac ccaaacacct acgactacat gaacaagcga gtggtggctc 21180 ccgggttagt ggactgctac attaaccttg gagcacgctg gtcccttgac tatatggaca 21240 acgtcaaccc atttaaccac caccgcaatg ctggcctgcg ctaccgctca atgttgctgg 21300 gcaatggtcg ctatgtgccc ttccacatcc aggtgcctca gaagttcttt gccattaaaa 21360 acctccttct cctgccgggc tcatacacct acgagtggaa cttcaggaag gatgttaaca 21420 tggttctgca gagctcccta ggaaatgacc taagggttga cggagccagc attaagtttg 21480 atagcatttg cctttacgcc accttcttcc ccatggccca caacaccgcc tccacgcttg 21540 aggccatgct tagaaacgac accaacgacc agtcctttaa cgactatctc tccgccgcca 21600 acatgctcta ccctataccc gccaacgcta ccaacgtgcc catatccatc ccctcccgca 21660 actgggcggc tttccgcggc tgggccttca cgcgccttaa gactaaggaa accccatcac 21720 tgggctcggg ctacgaccct tattacacct actctggctc tataccctac ctagatggaa 21780 ccttttacct caaccacacc tttaagaagg tggccattac ctttgactct tctgtcagct 21840 ggcctggcaa tgaccgcctg cttaccccca acgagtttga aattaagcgc tcagttgacg 21900 gggagggtta caacgttgcc cagtgtaaca tgaccaaaga ctggttcctg gtacaaatgc 21960 tagctaacta caacattggc taccagggct tctatatccc agagagctac aaggaccgca 22020 tgtactcctt ctttagaaac ttccagccca tgagccgtca ggtggtggat gatactaaat 22080 acaaggacta ccaacaggtg ggcatcctac accaacacaa caactctgga tttgttggct 22140 accttgcccc caccatgcgc gaaggacagg cctaccctgc taacttcccc tatccgctta 22200 taggcaagac cgcagttgac agcattaccc agaaaaagtt tctttgcgat cgcacccttt 22260 ggcgcatccc attctccagt aactttatgt ccatgggcgc actcacagac ctgggccaaa 22320 accttctcta cgccaactcc gcccacgcgc tagacatgac ttttgaggtg gatcccatgg 22380 acgagcccac ccttctttat gttttgtttg aagtctttga cgtggtccgt gtgcaccggc 22440 cgcaccgcgg cgtcatcgaa accgtgtacc tgcgcacgcc cttctcggcc ggcaacgcca 22500 caacataaag aagcaagcaa catcaacaac agctgccgcc atgggctcca gtgagcagga 22560 actgaaagcc attgtcaaag atcttggttg tgggccatat tttttgggca cctatgacaa 22620 gcgctttcca ggctttgttt ctccacacaa gctcgcctgc gccatagtca atacggccgg 22680 tcgcgagact gggggcgtac actggatggc ctttgcctgg aacccgcact caaaaacatg 22740 ctacctcttt gagccctttg gcttttctga ccagcgactc aagcaggttt accagtttga 22800 gtacgagtca ctcctgcgcc gtagcgccat tgcttcttcc cccgaccgct gtataacgct 22860 ggaaaagtcc acccaaagcg tacaggggcc caactcggcc gcctgtggac tattctgctg 22920 catgtttctc cacgcctttg ccaactggcc ccaaactccc atggatcaca accccaccat 22980 gaaccttatt accggggtac ccaactccat gctcaacagt ccccaggtac agcccaccct 23040 gcgtcgcaac caggaacagc tctacagctt cctggagcgc cactcgccct acttccgcag 23100 ccacagtgcg cagattagga gcgccacttc tttttgtcac ttgaaaaaca tgtaaaaata 23160 atgtactaga gacactttca ataaaggcaa atgcttttat ttgtacactc tcgggtgatt 23220 atttaccccc acccttgccg tctgcgccgt ttaaaaatca aaggggttct gccgcgcatc 23280 gctatgcgcc actggcaggg acacgttgcg atactggtgt ttagtgctcc acttaaactc 23340 aggcacaacc atccgcggca gctcggtgaa gttttcactc cacaggctgc gcaccatcac 23400 caacgcgttt agcaggtcgg gcgccgatat cttgaagtcg cagttggggc ctccgccctg 23460 cgcgcgcgag ttgcgataca cagggttgca gcactggaac actatcagcg ccgggtggtg 23520 cacgctggcc agcacgctct tgtcggagat cagatccgcg tccaggtcct ccgcgttgct 23580 cagggcgaac ggagtcaact ttggtagctg ccttcccaaa aagggcgcgt gcccaggctt 23640 tgagttgcac tcgcaccgta gtggcatcaa aaggtgaccg tgcccggtct gggcgttagg 23700 atacagcgcc tgcataaaag ccttgatctg cttaaaagcc acctgagcct ttgcgccttc 23760 agagaagaac atgccgcaag acttgccgga aaactgattg gccggacagg ccgcgtcgtg 23820 cacgcagcac cttgcgtcgg tgttggagat ctgcaccaca tttcggcccc accggttctt 23880 cacgatcttg gccttgctag actgctcctt cagcgcgcgc tgcccgtttt cgctcgtcac 23940 atccatttca atcacgtgct ccttatttat cataatgctt ccgtgtagac acttaagctc 24000 gccttcgatc tcagcgcagc ggtgcagcca caacgcgcag cccgtgggct cgtgatgctt 24060 gtaggtcacc tctgcaaacg actgcaggta cgcctgcagg aatcgcccca tcatcgtcac 24120 aaaggtcttg ttgctggtga aggtcagctg caacccgcgg tgctcctcgt tcagccaggt 24180 cttgcatacg gccgccagag cttccacttg gtcaggcagt agtttgaagt tcgcctttag 24240 atcgttatcc acgtggtact tgtccatcag cgcgcgcgca gcctccatgc ccttctccca 24300 cgcagacacg atcggcacac tcagcgggtt catcaccgta atttcacttt ccgcttcgct 24360 gggctcttcc tcttcctctt gcgtccgcat accacgcgcc actgggtcgt cttcattcag 24420 ccgccgcact gtgcgcttac ctcctttgcc atgcttgatt agcaccggtg ggttgctgaa 24480 acccaccatt tgtagcgcca catcttctct ttcttcctcg ctgtccacga ttacctctgg 24540 tgatggcggg cgctcgggct tgggagaagg gcgcttcttt ttcttcttgg gcgcaatggc 24600 caaatccgcc gccgaggtcg atggccgcgg gctgggtgtg cgcggcacca gcgcgtcttg 24660 tgatgagtct tcctcgtcct cggactcgat acgccgcctc atccgctttt ttgggggcgc 24720 ccggggaggc ggcggcgacg gggacgggga cgacacgtcc tccatggttg ggggacgtcg 24780 cgccgcaccg cgtccgcgct cgggggtggt ttcgcgctgc tcctcttccc gactggccat 24840 ttccttctcc tataggcaga aaaagatcat ggagtcagtc gagaagaagg acagcctaac 24900 cgccccctct gagttcgcca ccaccgcctc caccgatgcc gccaacgcgc ctaccacctt 24960 ccccgtcgag gcacccccgc ttgaggagga ggaagtgatt atcgagcagg acccaggttt 25020 tgtaagcgaa gacgacgagg accgctcagt accaacagag gataaaaagc aagaccagga 25080 caacgcagag gcaaacgagg aacaagtcgg gcggggggac gaaaggcatg gcgactacct 25140 agatgtggga gacgacgtgc tgttgaagca tctgcagcgc cagtgcgcca ttatctgcga 25200 cgcgttgcaa gagcgcagcg atgtgcccct cgccatagcg gatgtcagcc ttgcctacga 25260 acgccaccta ttctcaccgc gcgtaccccc caaacgccaa gaaaacggca catgcgagcc 25320 caacccgcgc ctcaacttct accccgtatt tgccgtgcca gaggtgcttg ccacctatca 25380 catctttttc caaaactgca agatacccct atcctgccgt gccaaccgca gccgagcgga 25440 caagcagctg gccttgcggc agggcgctgt catacctgat atcgcctcgc tcaacgaagt 25500 gccaaaaatc tttgagggtc ttggacgcga cgagaagcgc gcggcaaacg ctctgcaaca 25560 ggaaaacagc gaaaatgaaa gtcactctgg agtgttggtg gaactcgagg gtgacaacgc 25620 gcgcctagcc gtactaaaac gcagcatcga ggtcacccac tttgcctacc cggcacttaa 25680 cctacccccc aaggtcatga gcacagtcat gagtgagctg atcgtgcgcc gtgcgcagcc 25740 cctggagagg gatgcaaatt tgcaagaaca aacagaggag ggcctacccg cagttggcga 25800 cgagcagcta gcgcgctggc ttcaaacgcg cgagcctgcc gacttggagg agcgacgcaa 25860 actaatgatg gccgcagtgc tcgttaccgt ggagcttgag tgcatgcagc ggttctttgc 25920 tgacccggag atgcagcgca agctagagga aacattgcac tacacctttc gacagggcta 25980 cgtacgccag gcctgcaaga tctccaacgt ggagctctgc aacctggtct cctaccttgg 26040 aattttgcac gaaaaccgcc ttgggcaaaa cgtgcttcat tccacgctca agggcgaggc 26100 gcgccgcgac tacgtccgcg actgcgttta cttatttcta tgctacacct ggcagacggc 26160 catgggcgtt tggcagcagt gcttggagga gtgcaacctc aaggagctgc agaaactgct 26220 aaagcaaaac ttgaaggacc tatggacggc cttcaacgag cgctccgtgg ccgcgcacct 26280 ggcggacatc attttccccg aacgcctgct taaaaccctg caacagggtc tgccagactt 26340 caccagtcaa agcatgttgc agaactttag gaactttatc ctagagcgct caggaatctt 26400 gcccgccacc tgctgtgcac ttcctagcga ctttgtgccc attaagtacc gcgaatgccc 26460 tccgccgctt tggggccact gctaccttct gcagctagcc aactaccttg cctaccactc 26520 tgacataatg gaagacgtga gcggtgacgg tctactggag tgtcactgtc gctgcaacct 26580 atgcaccccg caccgctccc tggtttgcaa ttcgcagctg cttaacgaaa gtcaaattat 26640 cggtaccttt gagctgcagg gtccctcgcc tgacgaaaag tccgcggctc cggggttgaa 26700 actcactccg gggctgtgga cgtcggctta ccttcgcaaa tttgtacctg aggactacca 26760 cgcccacgag attaggttct acgaagacca atcccgcccg ccaaatgcgg agcttaccgc 26820 ctgcgtcatt acccagggcc acattcttgg ccaattgcaa gccatcaaca aagcccgcca 26880 agagtttctg ctacgaaagg gacggggggt ttacttggac ccccagtccg gcgaggagct 26940 caacccaatc cccccgccgc cgcagcccta tcagcagcag ccgcgggccc ttgcttccca 27000 ggatggcacc caaaaagaag ctgcagctgc cgccgccacc cacggacgag gaggaatact 27060 gggacagtca ggcagaggag gttttggacg aggaggagga ggacatgatg gaagactggg 27120 agagcctaga cgaggaagct tccgaggtcg aagaggtgtc agacgaaaca ccgtcaccct 27180 cggtcgcatt cccctcgccg gcgccccaga aatcggcaac cggttccagc atggctacaa 27240 cctccgctcc tcaggcgccg ccggcactgc ccgttcgccg acccaaccgt agatgggaca 27300 ccactggaac cagggccggt aagtccaagc agccgccgcc gttagcccaa gagcaacaac 27360 agcgccaagg ctaccgctca tggcgcgggc acaagaacgc catagttgct tgcttgcaag 27420 actgtggggg caacatctcc ttcgcccgcc gctttcttct ctaccatcac ggcgtggcct 27480 tcccccgtaa catcctgcat tactaccgtc atctctacag cccatactgc accggcggca 27540 gcggcagcgg cagcaacagc agcggccaca cagaagcaaa ggcgaccgga tagcaagact 27600 ctgacaaagc ccaagaaatc cacagcggcg gcagcagcag gaggaggagc gctgcgtctg 27660 gcgcccaacg aacccgtatc gacccgcgag cttagaaaca ggatttttcc cactctgtat 27720 gctatatttc aacagagcag gggccaagaa caagagctga aaataaaaaa caggtctctg 27780 cgatccctca cccgcagctg cctgtatcac aaaagcgaag atcagcttcg gcgcacgctg 27840 gaagacgcgg aggctctctt cagtaaatac tgcgcgctga ctcttaagga ctagtttcgc 27900 gccctttctc aaatttaagc gcgaaaacta cgtcatctcc agcggccaca cccggcgcca 27960 gcacctgtcg tcagcgccat ttcaactttg tatacaaaag ttgtgatgag caaggaaatt 28020 cccacgccct acatgtggag ttaccagcca caaatgggac ttgcggctgg agctgcccaa 28080 gactactcaa cccgaataaa ctacatgagc gcgggacccc acatgatatc ccgggtcaac 28140 ggaatacgcg cccaccgaaa ccgaattctc ctggaacagg cggctattac caccacacct 28200 cgtaataacc ttaatccccg tagttggccc gctgccctgg tgtaccagga aagtcccgct 28260 cccaccactg tggtacttcc cagagacgcc caggccgaag ttcagatgac taactcaggg 28320 gcgcagcttg cgggcggctt tcgtcacagg gtgcggtcgc ccgggcaggg tataactcac 28380 ctgacaatca gagggcgagg tattcagctc aacgacgagt cggtgagctc ctcgcttggt 28440 ctccgtccgg acgggacatt tcagatcggc ggcgccggcc gctcttcatt cacgcctcgt 28500 caggcaatcc taactctgca gacctcgtcc tctgagccgc gctctggagg cattggaact 28560 ctgcaattta ttgaggagtt tgtgccatcg gtctacttta accccttctc gggacctccc 28620 ggccactatc cggatcaatt tattcctaac tttgacgcgg taaaggactc ggcggacggc 28680 tacgactgaa tgttaagtgg agaggcagag caactgcgcc tgaaacacct ggtccactgt 28740 cgccgccaca agtgctttgc ccgcgactcc ggtgagtttt gctactttga attgcccgag 28800 gatcatatcg agggcccggc gcacggcgtc cggcttaccg cccagggaga gcttgcccgt 28860 agcctgattc gggagtttac ccagcgcccc ctgctagttg agcgggacag gggaccctgt 28920 gttctcactg tgatttgcaa ctgtcctaac cctggattac atcaagatct ttgttgccat 28980 ctctgtgctg agtataataa atacagaaat taaaatatac tggggctcct atcgccatcc 29040 tgtaaacgcc accgtcttca cccgcccaag caaaccaagg cgaaccttac ctggtacttt 29100 taacatctct ccctctgtga tttacaacag tttcaaccca gacggagtga gtctacgaga 29160 gaacctctcc gagctcagct actccatcag aaaaaacacc accctcctta cctgccggga 29220 acgtacgagt gcgtcaccgg ccgctgcacc acacctaccg cctgaccgta aaccagactt 29280 tttccggaca gacctcaata actctgttta ccagaacagg aggtgagctt agaaaaccct 29340 tagggtatta ggccaaaggc gcagctactg tggggtttat gaacaattca agcaactcta 29400 cgggctattc taattcaggt ttctctagaa tcggggttgg ggttattctc tgtcttgtga 29460 ttctctttat tcttatacta acgcttctct gcctaaggct cgccgcctgc tgtgtgcaca 29520 tttgcattta ttgtcagctt tttaaacgct ggggtcgcca cccaagatga ttaggtacat 29580 aatcctaggt ttactcaccc ttgcgtcagc ccacggtacc acccaaaagg tggattttaa 29640 ggagccagcc tgtaatgtta cattcgcagc tgaagctaat gagtgcacca ctcttataaa 29700 atgcaccaca gaacatgaaa agctgcttat tcgccacaaa aacaaaattg gcaagtatgc 29760 tgtttatgct atttggcagc caggtgacac tacagagtat aatgttacag ttttccaggg 29820 taaaagtcat aaaactttta tgtatacttt tccattttat gaaatgtgcg acattaccat 29880 gtacatgagc aaacagtata agttgtggcc cccacaaaat tgtgtggaaa acactggcac 29940 tttctgctgc actgctatgc taattacagt gctcgctttg gtctgtaccc tactctatat 30000 taaatacaaa agcagacgca gctttattga ggaaaagaaa atgccttaat ttactaagtt 30060 acaaagctaa tgtcaccact aactgcttta ctcgctgctt gcaaaacaaa ttcaaaaagt 30120 tagcattata attagaatag gatttaaacc ccccggtcat ttcctgctca ataccattcc 30180 cctgaacaat tgactctatg tgggatatgc tccagcgcta caaccttgaa gtcaggcttc 30240 ctggatgtca gcatctgact ttggccagca cctgtcccgc ggatttgttc cagtccaact 30300 acagcgaccc accctaacag agatgaccaa cacaaccaac gcggccgccg ctaccggact 30360 tacatctacc acaaatacac cccaagtttc tgcctttgtc aataactggg ataacttggg 30420 catgtggtgg ttctccatag cgcttatgtt tgtatgcctt attattatgt ggctcatctg 30480 ctgcctaaag cgcaaacgcg cccgaccacc catctatagt cccatcattg tgctacaccc 30540 aaacaatgat ggaatccata gattggacgg actgaaacac atgttctttt ctcttacagt 30600 atgattaaat gagacatgat tcctcgagtt tttatattac tgacccttgt tgcgcttttt 30660 tgtgcgtgct ccacattggc tgcggtttct cacatcgaag tagactgcat tccagccttc 30720 acagtctatt tgctttacgg atttgtcacc ctcacgctca tctgcagcct catcactgtg 30780 gtcatcgcct ttatccagtg cattgactgg gtctgtgtgc gctttgcata tctcagacac 30840 catccccagt acagggacag gactatagct gagcttctta gaattcttta attatgaaat 30900 ttactgtgac ttttctgctg attatttgca ccctatctgc gttttgttcc ccgacctcca 30960 agcctcaaag acatatatca tgcagattca ctcgtatatg gaatattcca agttgctaca 31020 atgaaaaaag cgatctttcc gaagcctggt tatatgcaat catctctgtt atggtgttct 31080 gcagtaccat cttagcccta gctatatatc cctaccttga cattggctgg aacgcaatag 31140 atgccatgaa ccacccaact ttccccgcgc ccgctatgct tccactgcaa caagttgttg 31200 ccggcggctt tgtcccagcc aatcagcctc gcccaccttc tcccaccccc actgaaatca 31260 gctactttaa tctaacagga ggagatgact gacaccctag atctagaaat ggacggaatt 31320 attacagagc agcgcctgct agaaagacgc agggcagcgg ccgagcaaca gcgcatgaat 31380 caagagctcc aagacatggt taacttgcac cagtgcaaaa ggggtatctt ttgtctggta 31440 aagcaggcca aagtcaccta cgacagtaat accaccggac accgccttag ctacaagttg 31500 ccaaccaagc gtcagaaatt ggtggtcatg gtgggagaaa agcccattac cataactcag 31560 cactcggtag aaaccgaagg ctgcattcac tcaccttgtc aaggacctga ggatctctgc 31620 acccttatta agaccctgtg cggtctcaaa gatcttattc cctttaacta ataaaaaaaa 31680 ataataaagc atcacttact taaaatcagt tagcaaattt ctgtccagtt tattcagcag 31740 cacctccttg ccctcctccc agctctggta ttgcagcttc ctcctggctg caaactttct 31800 ccacaatcta aatggaatgt cagtttcctc ctgttcctgt ccatccgcac ccactatctt 31860 catgttgttg cagatgaagc gcgcaagacc gtctgaagat accttcaacc ccgtgtatcc 31920 atatgacacg gaaaccggtc ctccaactgt gccttttctt actcctccct ttgtatcccc 31980 caatgggttt caagagagtc cccctggggt actctctttg cgcctatccg aacctctagt 32040 tacctccaat ggcatgcttg cgctcaaaat gggcaacggc ctctctctgg acgaggccgg 32100 caaccttacc tcccaaaatg taaccactgt gagcccacct ctcaaaaaaa ccaagtcaaa 32160 cataaacctg gaaatatctg cacccctcac agttacctca gaagccctaa ctgtggctgc 32220 cgccgcacct ctaatggtcg cgggcaacac actcaccatg caatcacagg ccccgctaac 32280 cgtgcacgac tccaaactta gcattgccac ccaaggaccc ctcacagtgt cagaaggaaa 32340 gctagccctg caaacatcag gccccctcac caccaccgat agcagtaccc ttactatcac 32400 tgcctcaccc cctctaacta ctgccactgg tagcttgggc attgacttga aagagcccat 32460 ttatacacaa aatggaaaac taggactaaa gtacggggct cctttgcatg taacagacga 32520 cctaaacact ttgaccgtag caactggtcc aggtgtgact attaataata cttccttgca 32580 aactaaagtt actggagcct tgggttttga ttcacaaggc aatatgcaac ttaatgtagc 32640 aggaggacta aggattgatt ctcaaaacag acgccttata cttgatgtta gttatccgtt 32700 tgatgctcaa aaccaactaa atctaagact aggacagggc cctcttttta taaactcagc 32760 ccacaacttg gatattaact acaacaaagg cctttacttg tttacagctt caaacaattc 32820 caaaaagctt gaggttaacc taagcactgc caaggggttg atgtttgacg ctacagccat 32880 agccattaat gcaggagatg ggcttgaatt tggttcacct aatgcaccaa acacaaatcc 32940 cctcaaaaca aaaattggcc atggcctaga atttgattca aacaaggcta tggttcctaa 33000 actaggaact ggccttagtt ttgacagcac aggtgccatt acagtaggaa acaaaaataa 33060 tgataagcta actttgtgga ccacaccagc tccatctcct aactgtagac taaatgcaga 33120 gaaagatgct aaactcactt tggtcttaac aaaatgtggc agtcaaatac ttgctacagt 33180 ttcagttttg gctgttaaag gcagtttggc tccaatatct ggaacagttc aaagtgctca 33240 tcttattata agatttgacg aaaatggagt gctactaaac aattccttcc tggacccaga 33300 atattggaac tttagaaatg gagatcttac tgaaggcaca gcctatacaa acgctgttgg 33360 atttatgcct aacctatcag cttatccaaa atctcacggt aaaactgcca aaagtaacat 33420 tgtcagtcaa gtttacttaa acggagacaa aactaaacct gtaacactaa ccattacact 33480 aaacggtaca caggaaacag gagacacaac tccaagtgca tactctatgt cattttcatg 33540 ggactggtct ggccacaact acattaatga aatatttgcc acatcctctt acactttttc 33600 atacattgcc caagaataaa gaatcgtttg tgttatgttt caacgtgttt atttttcaat 33660 tgcagaaaat ttcaagtcat ttttcattca gtagtatagc cccaccacca catagcttat 33720 acagatcacc gtacctcaac tttgtataat aaagttgtaa tcaaactcac agaaccctag 33780 tattcaacct gccacctccc tcccaacaca cagagtacac agtcctttct ccccggctgg 33840 ccttaaaaag catcatatca tgggtaacag acatattctt aggtgttata ttccacacgg 33900 tttcctgtcg agccaaacgc tcatcagtga tattaataaa ctccccgggc agctcactta 33960 agttcatgtc gctgtccagc tgctgagcca caggctgctg tccaacttgc ggttgcttaa 34020 cgggcggcga aggagaagtc cacgcctaca tgggggtaga gtcataatcg tgcatcagga 34080 tagggcggtg gtgctgcagc agcgcgcgaa taaactgctg ccgccgccgc tccgtcctgc 34140 aggaatacaa catggcagtg gtctcctcag cgatgattcg caccgcccgc agcataaggc 34200 gccttgtcct ccgggcacag cagcgcaccc tgatctcact taaatcagca cagtaactgc 34260 agcacagcac cacaatattg ttcaaaatcc cacagtgcaa ggcgctgtat ccaaagctca 34320 tggcggggac cacagaaccc acgtggccat cataccacaa gcgcaggtag attaagtggc 34380 gacccctcat aaacacgctg gacataaaca ttacctcttt tggcatgttg taattcacca 34440 cctcccggta ccatataaac ctctgattaa acatggcgcc atccaccacc atcctaaacc 34500 agctggccaa aacctgcccg ccggctatac actgcaggga accgggactg gaacaatgac 34560 agtggagagc ccaggactcg taaccatgga tcatcatgct cgtcatgata tcaatgttgg 34620 cacaacacag gcacacgtgc atacacttcc tcaggattac aagctcctcc cgcgttagaa 34680 ccatatccca gggaacaacc cattcctgaa tcagcgtaaa tcccacactg cagggaagac 34740 ctcgcacgta actcacgttg tgcattgtca aagtgttaca ttcgggcagc agcggatgat 34800 cctccagtat ggtagcgcgg gtttctgtct caaaaggagg tagacgatcc ctactgtacg 34860 gagtgcgccg agacaaccga gatcgtgttg gtcgtagtgt catgccaaat ggaacgccgg 34920 acgtagtcat atttcctgaa gcaaaaccag gtgcgggcgt gacaaacaga tctgcgtctc 34980 cggtctcgcc gcttagatcg ctctgtgtag tagttgtagt atatccactc tctcaaagca 35040 tccaggcgcc ccctggcttc gggttctatg taaactcctt catgcgccgc tgccctgata 35100 acatccacca ccgcagaata agccacaccc agccaaccta cacattcgtt ctgcgagtca 35160 cacacgggag gagcgggaag agctggaaga accatgtttt tttttttatt ccaaaagatt 35220 atccaaaacc tcaaaatgaa gatctattaa gtgaacgcgc tcccctccgg tggcgtggtc 35280 aaactctaca gccaaagaac agataatggc atttgtaaga tgttgcacaa tggcttccaa 35340 aaggcaaacg gccctcacgt ccaagtggac gtaaaggcta aacccttcag ggtgaatctc 35400 ctctataaac attccagcac cttcaaccat gcccaaataa ttctcatctc gccaccttct 35460 caatatatct ctaagcaaat cccgaatatt aagtccggcc attgtaaaaa tctgctccag 35520 agcgccctcc accttcagcc tcaagcagcg aatcatgatt gcaaaaattc aggttcctca 35580 cagacctgta taagattcaa aagcggaaca ttaacaaaaa taccgcgatc ccgtaggtcc 35640 cttcgcaggg ccagctgaac ataatcgtgc aggtctgcac ggaccagcgc ggccacttcc 35700 ccgccaggaa ccatgacaaa agaacccaca ctgattatga cacgcatact cggagctatg 35760 ctaaccagcg tagccccgat gtaagcttgt tgcatgggcg gcgatataaa atgcaaggtg 35820 ctgctcaaaa aatcaggcaa agcctcgcgc aaaaaagaaa gcacatcgta gtcatgctca 35880 tgcagataaa ggcaggtaag ctccggaacc accacagaaa aagacaccat ttttctctca 35940 aacatgtctg cgggtttctg cataaacaca aaataaaata acaaaaaaac atttaaacat 36000 tagaagcctg tcttacaaca ggaaaaacaa cccttataag cataagacgg actacggcca 36060 tgccggcgtg accgtaaaaa aactggtcac cgtgattaaa aagcaccacc gacagctcct 36120 cggtcatgtc cggagtcata atgtaagact cggtaaacac atcaggttga ttcacatcgg 36180 tcagtgctaa aaagcgaccg aaatagcccg ggggaataca tacccgcagg cgtagagaca 36240 acattacagc ccccatagga ggtataacaa aattaatagg agagaaaaac acataaacac 36300 ctgaaaaacc ctcctgccta ggcaaaatag caccctcccg ctccagaaca acatacagcg 36360 cttccacagc ggcagccata acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa 36420 aacaccactc gacacggcac cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag 36480 agcgagtata tataggacta aaaaatgacg taacggttaa agtccacaaa aaacacccag 36540 aaaaccgcac gcgaacctac gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa 36600 tcgtcacttc cgttttccca cgttacgtca cttcccattt taagaaaact acaattccca 36660 acacatacaa gttactccgc cctaaaacct acgtcacccg ccccgttccc acgccccgcg 36720 ccacgtcaca aactccaccc cctcattatc atattggctt caatccaaaa taaggtatat 36780 tattgatgat g 36791 <210> 4 <211> 36782 <212> DNA <213> Artificial Sequence <220> <223> Synthetic adenovirus genome E1B-55k-P2A-mCherry <400> 4 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420 cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480 tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540 tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600 aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660 tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720 cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780 gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840 cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900 ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960 cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020 caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080 ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140 tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200 gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260 ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320 cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380 ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440 gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500 cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560 ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620 gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680 cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740 ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800 tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860 gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920 caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980 gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040 agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100 aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160 cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220 gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280 gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340 gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400 gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460 tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520 ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580 tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640 agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700 tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760 gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820 acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880 gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940 aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000 ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060 gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120 tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180 acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240 aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300 tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360 gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420 tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480 ttggctctag cgatgaagat acagatggaa gcggagctac taacttcagc ctgctgaagc 3540 aggctggtga cgtcgaggag aatcctggcc caatggtgag caagggcgag gaggataaca 3600 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc 3660 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca 3720 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt 3780 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc 3840 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg 3900 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc 3960 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg 4020 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc 4080 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga 4140 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca 4200 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg 4260 gcatggacga gctgtacaag tgaggtactg aaatgtgtgg gcgtggctta agggtgggaa 4320 agaatatata aggtgggggt cttatgtagt tttgtatctg ttttgcagca gccgccgccg 4380 cccaactttt ctatacaaag ttgtaatgag caccaactcg tttgatggaa gcattgtgag 4440 ctcatatttg acaacgcgca tgcccccatg ggccggggtg cgtcagaatg tgatgggctc 4500 cagcattgat ggtcgccccg tcctgcccgc aaactctact accttgacct acgagaccgt 4560 gtctggaacg ccgttggaga ctgcagcctc cgccgccgct tcagccgctg cagccaccgc 4620 ccgcgggatt gtgactgact ttgctttcct gagcccgctt gcaagcagtg cagcttcccg 4680 ttcatccgcc cgcgatgaca agttgacggc tcttttggca caattggatt ctttgacccg 4740 ggaacttaat gtcgtttctc agcagctgtt ggatctgcgc cagcaggttt ctgccctgaa 4800 ggcttcctcc cctcccaatg cggtttaaaa cataaataaa aaaccagact ctgtttggat 4860 ttggatcaag cataagtgtc ttgctgtctt tatttagggg ttttgcgcgc gcggtaggcc 4920 cgggaccagc ggtctcggtc gttgagggtc ctgtgtattt tttccaggac gtggtaaagg 4980 tgactctgga tgttcagata catgggcata agcccgtctc tggggtggag gtagcaccac 5040 tgcagagctt catgctgcgg ggtggtgttg tagatgatcc agtcgtagca ggagcgctgg 5100 gcgtggtgcc taaaaatgtc tttcagtagc aagctgattg ccaggggcag gcccttggtg 5160 taagtgttta caaagcggtt aagctgggat gggtgcatac gtggggatat gagatgcatc 5220 ttggactgta tttttaggtt ggctatgttc ccagccatat ccctccgggg attcatgttg 5280 tgcagaacca ccagcacagt gtatccggtg cacttgggaa atttgtcatg tagcttagaa 5340 ggaaatgcgt ggaagaactt ggagacgccc ttgtgacctc caagattttc catgcattcg 5400 tccataatga tggcaatggg cccacgggcg gcggcctggg cgaagatatt tctgggatca 5460 ctaacgtcat agttgtgttc caggatgaga tcgtcatagg ccatttttac aaagcgcggg 5520 cggagggtgc cagactgcgg tataatggtt ccatccggcc caggggcgta gttaccctca 5580 cagatttgca tttcccacgc tttgagttca gatgggggga tcatgtctac ctgcggggcg 5640 atgaagaaaa cggtttccgg ggtaggggag atcagctggg aagaaagcag gttcctgagc 5700 agctgcgact taccgcagcc ggtgggcccg taaatcacac ctattaccgg gtgcaactgg 5760 tagttaagag agctgcagct gccgtcatcc ctgagcaggg gggccacttc gttaagcatg 5820 tccctgactc gcatgttttc cctgaccaaa tccgccagaa ggcgctcgcc gcccagcgat 5880 agcagttctt gcaaggaagc aaagtttttc aacggtttga gaccgtccgc cgtaggcatg 5940 cttttgagcg tttgaccaag cagttccagg cggtcccaca gctcggtcac ctgctctacg 6000 gcatctcgat ccagcatatc tcctcgtttc gcgggttggg gcggctttcg ctgtacggca 6060 gtagtcggtg ctcgtccaga cgggccaggg tcatgtcttt ccacgggcgc agggtcctcg 6120 tcagcgtagt ctgggtcacg gtgaaggggt gcgctccggg ctgcgcgctg gccagggtgc 6180 gcttgaggct ggtcctgctg gtgctgaagc gctgccggtc ttcgccctgc gcgtcggcca 6240 ggtagcattt gaccatggtg tcatagtcca gcccctccgc ggcgtggccc ttggcgcgca 6300 gcttgccctt ggaggaggcg ccgcacgagg ggcagtgcag acttttgagg gcgtagagct 6360 tgggcgcgag aaataccgat tccggggagt aggcatccgc gccgcaggcc ccgcagacgg 6420 tctcgcattc cacgagccag gtgagctctg gccgttcggg gtcaaaaacc aggtttcccc 6480 catgcttttt gatgcgtttc ttacctctgg tttccatgag ccggtgtcca cgctcggtga 6540 cgaaaaggct gtccgtgtcc ccgtatacag acttgagagg cctgtcctcg agcggtgttc 6600 cgcggtcctc ctcgtataga aactcggacc actctgagac aaaggctcgc gtccaggcca 6660 gcacgaagga ggctaagtgg gaggggtagc ggtcgttgtc cactaggggg tccactcgct 6720 ccagggtgtg aagacacatg tcgccctctt cggcatcaag gaaggtgatt ggtttgtagg 6780 tgtaggccac gtgaccgggt gttcctgaag gggggctata aaagggggtg ggggcgcgtt 6840 cgtcctcact ctcttccgca tcgctgtctg cgagggccag ctgttggggt gagtactccc 6900 tctgaaaagc gggcatgact tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt 6960 tgatattcac ctggcccgcg gtgatgcctt tgagggtggc cgcatccatc tggtcagaaa 7020 agacaatctt tttgttgtca agcttggtgg caaacgaccc gtagagggcg ttggacagca 7080 acttggcgat ggagcgcagg gtttggtttt tgtcgcgatc ggcgcgctcc ttggccgcga 7140 tgtttagctg cacgtattcg cgcgcaacgc accgccattc gggaaagacg gtggtgcgct 7200 cgtcgggcac caggtgcacg cgccaaccgc ggttgtgcag ggtgacaagg tcaacgctgg 7260 tggctacctc tccgcgtagg cgctcgttgg tccagcagag gcggccgccc ttgcgcgagc 7320 agaatggcgg tagggggtct agctgcgtct cgtccggggg gtctgcgtcc acggtaaaga 7380 ccccgggcag caggcgcgcg tcgaagtagt ctatcttgca tccttgcaag tctagcgcct 7440 gctgccatgc gcgggcggca agcgcgcgct cgtatgggtt gagtggggga ccccatggca 7500 tggggtgggt gagcgcggag gcgtacatgc cgcaaatgtc gtaaacgtag aggggctctc 7560 tgagtattcc aagatatgta gggtagcatc ttccaccgcg gatgctggcg cgcacgtaat 7620 cgtatagttc gtgcgaggga gcgaggaggt cgggaccgag gttgctacgg gcgggctgct 7680 ctgctcggaa gactatctgc ctgaagatgg catgtgagtt ggatgatatg gttggacgct 7740 ggaagacgtt gaagctggcg tctgtgagac ctaccgcgtc acgcacgaag gaggcgtagg 7800 agtcgcgcag cttgttgacc agctcggcgg tgacctgcac gtctagggcg cagtagtcca 7860 gggtttcctt gatgatgtca tacttatcct gtcccttttt tttccacagc tcgcggttga 7920 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac 7980 ggtaagagcc tagcatgtag aactggttga cggcctggta ggcgcagcat cccttttcta 8040 cgggtagcgc gtatgcctgc gcggccttcc ggagcgaggt gtgggtgagc gcaaaggtgt 8100 ccctgaccat gactttgagg tactggtatt tgaagtcagt gtcgtcgcat ccgccctgct 8160 cccagagcaa aaagtccgtg cgctttttgg aacgcggatt tggcagggcg aaggtgacat 8220 cgttgaagag tatctttccc gcgcgaggca taaagttgcg tgtgatgcgg aagggtcccg 8280 gcacctcgga acggttgtta attacctggg cggcgagcac gatctcgtca aagccgttga 8340 tgttgtggcc cacaatgtaa agttccaaga agcgcgggat gcccttgatg gaaggcaatt 8400 ttttaagttc ctcgtaggtg agctcttcag gggagctgag cccgtgctct gaaagggccc 8460 agtctgcaag atgagggttg gaagcgacga atgagctcca caggtcacgg gccattagca 8520 tttgcaggtg gtcgcgaaag gtcctaaact ggcgacctat ggccattttt tctggggtga 8580 tgcagtagaa ggtaagcggg tcttgttccc agcggtccca tccaaggttc gcggctaggt 8640 ctcgcgcggc agtcactaga ggctcatctc cgccgaactt catgaccagc atgaagggca 8700 cgagctgctt cccaaaggcc cccatccaag tataggtctc tacatcgtag gtgacaaaga 8760 gacgctcggt gcgaggatgc gagccgatcg ggaagaactg gatctcccgc caccaattgg 8820 aggagtggct attgatgtgg tgaaagtaga agtccctgcg acgggccgaa cactcgtgct 8880 ggcttttgta aaaacgtgcg cagtactggc agcggtgcac gggctgtaca tcctgcacga 8940 ggttgacctg acgaccgcgc acaaggaagc agagtgggaa tttgagcccc tcgcctggcg 9000 ggtttggctg gtggtcttct acttcggctg cttgtccttg accgtctggc tgctcgaggg 9060 gagttacggt ggatcggacc accacgccgc gcgagcccaa agtccagatg tccgcgcgcg 9120 gcggtcggag cttgatgaca acatcgcgca gatgggagct gtccatggtc tggagctccc 9180 gcggcgtcag gtcaggcggg agctcctgca ggtttacctc gcatagacgg gtcagggcgc 9240 gggctagatc caggtgatac ctaatttcca ggggctggtt ggtggcggcg tcgatggctt 9300 gcaagaggcc gcatccccgc ggcgcgacta cggtaccgcg cggcgggcgg tgggccgcgg 9360 gggtgtcctt ggatgatgca tctaaaagcg gtgacgcggg cgagcccccg gaggtagggg 9420 gggctccgga cccgccggga gagggggcag gggcacgtcg gcgccgcgcg cgggcaggag 9480 ctggtgctgc gcgcgtaggt tgctggcgaa cgcgacgacg cggcggttga tctcctgaat 9540 ctggcgcctc tgcgtgaaga cgacgggccc ggtgagcttg agcctgaaag agagttcgac 9600 agaatcaatt tcggtgtcgt tgacggcggc ctggcgcaaa atctcctgca cgtctcctga 9660 gttgtcttga taggcgatct cggccatgaa ctgctcgatc tcttcctcct ggagatctcc 9720 gcgtccggct cgctccacgg tggcggcgag gtcgttggaa atgcgggcca tgagctgcga 9780 gaaggcgttg aggcctccct cgttccagac gcggctgtag accacgcccc cttcggcatc 9840 gcgggcgcgc atgaccacct gcgcgagatt gagctccacg tgccgggcga agacggcgta 9900 gtttcgcagg cgctgaaaga ggtagttgag ggtggtggcg gtgtgttctg ccacgaagaa 9960 gtacataacc cagcgtcgca acgtggattc gttgatatcc cccaaggcct caaggcgctc 10020 catggcctcg tagaagtcca cggcgaagtt gaaaaactgg gagttgcgcg ccgacacggt 10080 taactcctcc tccagaagac ggatgagctc ggcgacagtg tcgcgcacct cgcgctcaaa 10140 ggctacaggg gcctcttctt cttcttcaat ctcctcttcc ataagggcct ccccttcttc 10200 ttcttctggc ggcggtgggg gaggggggac acggcggcga cgacggcgca ccgggaggcg 10260 gtcgacaaag cgctcgatca tctccccgcg gcgacggcgc atggtctcgg tgacggcgcg 10320 gccgttctcg cgggggcgca gttggaagac gccgcccgtc atgtcccggt tatgggttgg 10380 cggggggctg ccatgcggca gggatacggc gctaacgatg catctcaaca attgttgtgt 10440 aggtactccg ccgccgaggg acctgagcga gtccgcatcg accggatcgg aaaacctctc 10500 gagaaaggcg tctaaccagt cacagtcgca aggtaggctg agcaccgtgg cgggcggcag 10560 cgggcggcgg tcggggttgt ttctggcgga ggtgctgctg atgatgtaat taaagtaggc 10620 ggtcttgaga cggcggatgg tcgacagaag caccatgtcc ttgggtccgg cctgctgaat 10680 gcgcaggcgg tcggccatgc cccaggcttc gttttgacat cggcgcaggt ctttgtagta 10740 gtcttgcatg agcctttcta ccggcacttc ttcttctcct tcctcttgtc ctgcatctct 10800 tgcatctatc gctgcggcgg cggcggagtt tggccgtagg tggcgccctc ttcctcccat 10860 gcgtgtgacc ccgaagcccc tcatcggctg aagcagggct aggtcggcga caacgcgctc 10920 ggctaatatg gcctgctgca cctgcgtgag ggtagactgg aagtcatcca tgtccacaaa 10980 gcggtggtat gcgcccgtgt tgatggtgta agtgcagttg gccataacgg accagttaac 11040 ggtctggtga cccggctgcg agagctcggt gtacctgaga cgcgagtaag ccctcgagtc 11100 aaatacgtag tcgttgcaag tccgcaccag gtactggtat cccaccaaaa agtgcggcgg 11160 cggctggcgg tagaggggcc agcgtagggt ggccggggct ccgggggcga gatcttccaa 11220 cataaggcga tgatatccgt agatgtacct ggacatccag gtgatgccgg cggcggtggt 11280 ggaggcgcgc ggaaagtcgc ggacgcggtt ccagatgttg cgcagcggca aaaagtgctc 11340 catggtcggg acgctctggc cggtcaggcg cgcgcaatcg ttgacgctct agaccgtgca 11400 aaaggagagc ctgtaagcgg gcactcttcc gtggtctggt ggataaattc gcaagggtat 11460 catggcggac gaccggggtt cgagccccgt atccggccgt ccgccgtgat ccatgcggtt 11520 accgcccgcg tgtcgaaccc aggtgtgcga cgtcagacaa cgggggagtg ctccttttgg 11580 cttccttcca ggcgcggcgg ctgctgcgct agcttttttg gccactggcc gcgcgcagcg 11640 taagcggtta ggctggaaag cgaaagcatt aagtggctcg ctccctgtag ccggagggtt 11700 attttccaag ggttgagtcg cgggaccccc ggttcgagtc tcggaccggc cggactgcgg 11760 cgaacggggg tttgcctccc cgtcatgcaa gaccccgctt gcaaattcct ccggaaacag 11820 ggacgagccc cttttttgct tttcccagat gcatccggtg ctgcggcaga tgcgcccccc 11880 tcctcagcag cggcaagagc aagagcagcg gcagacatgc agggcaccct cccctcctcc 11940 taccgcgtca ggaggggcga catccgcggt tgacgcggca gcagatggtg attacgaacc 12000 cccgcggcgc cgggcccggc actacctgga cttggaggag ggcgagggcc tggcgcggct 12060 aggagcgccc tctcctgagc ggtacccaag ggtgcagctg aagcgtgata cgcgtgaggc 12120 gtacgtgccg cggcagaacc tgtttcgcga ccgcgaggga gaggagcccg aggagatgcg 12180 ggatcgaaag ttccacgcag ggcgcgagct gcggcatggc ctgaatcgcg agcggttgct 12240 gcgcgaggag gactttgagc ccgacgcgcg aaccgggatt agtcccgcgc gcgcacacgt 12300 ggcggccgcc gacctggtaa ccgcatacga gcagacggtg aaccaggaga ttaactttca 12360 aaaaagcttt aacaaccacg tgcgtacgct tgtggcgcgc gaggaggtgg ctataggact 12420 gatgcatctg tgggactttg taagcgcgct ggagcaaaac ccaaatagca agccgctcat 12480 ggcgcagctg ttccttatag tgcagcacag cagggacaac gaggcattca gggatgcgct 12540 gctaaacata gtagagcccg agggccgctg gctgctcgat ttgataaaca tcctgcagag 12600 catagtggtg caggagcgca gcttgagcct ggctgacaag gtggccgcca tcaactattc 12660 catgcttagc ctgggcaagt tttacgcccg caagatatac catacccctt acgttcccat 12720 agacaaggag gtaaagatcg aggggttcta catgcgcatg gcgctgaagg tgcttacctt 12780 gagcgacgac ctgggcgttt atcgcaacga gcgcatccac aaggccgtga gcgtgagccg 12840 gcggcgcgag ctcagcgacc gcgagctgat gcacagcctg caaagggccc tggctggcac 12900 gggcagcggc gatagagagg ccgagtccta ctttgacgcg ggcgctgacc tgcgctgggc 12960 cccaagccga cgcgccctgg aggcagctgg ggccggacct gggctggcgg tggcacccgc 13020 gcgcgctggc aacgtcggcg gcgtggagga atatgacgag gacgatgagt acgagccaga 13080 ggacggcgag tactaagcgg tgatgtttct gatcagatga tgcaagacgc aacggacccg 13140 gcggtgcggg cggcgctgca gagccagccg tccggcctta actccacgga cgactggcgc 13200 caggtcatgg accgcatcat gtcgctgact gcgcgcaatc ctgacgcgtt ccggcagcag 13260 ccgcaggcca accggctctc cgcaattctg gaagcggtgg tcccggcgcg cgcaaacccc 13320 acgcacgaga aggtgctggc gatcgtaaac gcgctggccg aaaacagggc catccggccc 13380 gacgaggccg gcctggtcta cgacgcgctg cttcagcgcg tggctcgtta caacagcggc 13440 aacgtgcaga ccaacctgga ccggctggtg ggggatgtgc gcgaggccgt ggcgcagcgt 13500 gagcgcgcgc agcagcaggg caacctgggc tccatggttg cactaaacgc cttcctgagt 13560 acacagcccg ccaacgtgcc gcggggacag gaggactaca ccaactttgt gagcgcactg 13620 cggctaatgg tgactgagac accgcaaagt gaggtgtacc agtctgggcc agactatttt 13680 ttccagacca gtagacaagg cctgcagacc gtaaacctga gccaggcttt caaaaacttg 13740 caggggctgt ggggggtgcg ggctcccaca ggcgaccgcg cgaccgtgtc tagcttgctg 13800 acgcccaact cgcgcctgtt gctgctgcta atagcgccct tcacggacag tggcagcgtg 13860 tcccgggaca catacctagg tcacttgctg acactgtacc gcgaggccat aggtcaggcg 13920 catgtggacg agcatacttt ccaggagatt acaagtgtca gccgcgcgct ggggcaggag 13980 gacacgggca gcctggaggc aaccctaaac tacctgctga ccaaccggcg gcagaagatc 14040 ccctcgttgc acagtttaaa cagcgaggag gagcgcattt tgcgctacgt gcagcagagc 14100 gtgagcctta acctgatgcg cgacggggta acgcccagcg tggcgctgga catgaccgcg 14160 cgcaacatgg aaccgggcat gtatgcctca aaccggccgt ttatcaaccg cctaatggac 14220 tacttgcatc gcgcggccgc cgtgaacccc gagtatttca ccaatgccat cttgaacccg 14280 cactggctac cgccccctgg tttctacacc gggggattcg aggtgcccga gggtaacgat 14340 ggattcctct gggacgacat agacgacagc gtgttttccc cgcaaccgca gaccctgcta 14400 gagttgcaac agcgcgagca ggcagaggcg gcgctgcgaa aggaaagctt ccgcaggcca 14460 agcagcttgt ccgatctagg cgctgcggcc ccgcggtcag atgctagtag cccatttcca 14520 agcttgatag ggtctcttac cagcactcgc accacccgcc cgcgcctgct gggcgaggag 14580 gagtacctaa acaactcgct gctgcagccg cagcgcgaaa aaaacctgcc tccggcattt 14640 cccaacaacg ggatagagag cctagtggac aagatgagta gatggaagac gtacgcgcag 14700 gagcacaggg acgtgccagg cccgcgcccg cccacccgtc gtcaaaggca cgaccgtcag 14760 cggggtctgg tgtgggagga cgatgactcg gcagacgaca gcagcgtcct ggatttggga 14820 gggagtggca acccgtttgc gcaccttcgc cccaggctgg ggagaatgtt ttaaaaaaaa 14880 aaaagcatga tgcaaaataa aaaactcacc aaggccatgg caccgagcgt tggttttctt 14940 gtattcccct tagtatgcgg cgcgcggcga tgtatgagga aggtcctcct ccctcctacg 15000 agagtgtggt gagcgcggcg ccagtggcgg cggcgctggg ttctcccttc gatgctcccc 15060 tggacccgcc gtttgtgcct ccgcggtacc tgcggcctac cggggggaga aacagcatcc 15120 gttactctga gttggcaccc ctattcgaca ccacccgtgt gtacctggtg gacaacaagt 15180 caacggatgt ggcatccctg aactaccaga acgaccacag caactttctg accacggtca 15240 ttcaaaacaa tgactacagc ccgggggagg caagcacaca gaccatcaat cttgacgacc 15300 ggtcgcactg gggcggcgac ctgaaaacca tcctgcatac caacatgcca aatgtgaacg 15360 agttcatgtt taccaataag tttaaggcgc gggtgatggt gtcgcgcttg cctactaagg 15420 acaatcaggt ggagctgaaa tacgagtggg tggagttcac gctgcccgag ggcaactact 15480 ccgagaccat gaccatagac cttatgaaca acgcgatcgt ggagcactac ttgaaagtgg 15540 gcagacagaa cggggttctg gaaagcgaca tcggggtaaa gtttgacacc cgcaacttca 15600 gactggggtt tgaccccgtc actggtcttg tcatgcctgg ggtatataca aacgaagcct 15660 tccatccaga catcattttg ctgccaggat gcggggtgga cttcacccac agccgcctga 15720 gcaacttgtt gggcatccgc aagcggcaac ccttccagga gggctttagg atcacctacg 15780 atgatctgga gggtggtaac attcccgcac tgttggatgt ggacgcctac caggcgagct 15840 tgaaagatga caccgaacag ggcgggggtg gcgcaggcgg cagcaacagc agtggcagcg 15900 gcgcggaaga gaactccaac gcggcagccg cggcaatgca gccggtggag gacatgaacg 15960 atcatgccat tcgcggcgac acctttgcca cacgggctga ggagaagcgc gctgaggccg 16020 aagcagcggc cgaagctgcc gcccccgctg cgcaacccga ggtcgagaag cctcagaaga 16080 aaccggtgat caaacccctg acagaggaca gcaagaaacg cagttacaac ctaataagca 16140 atgacagcac cttcacccag taccgcagct ggtaccttgc atacaactac ggcgaccctc 16200 agaccggaat ccgctcatgg accctgcttt gcactcctga cgtaacctgc ggctcggagc 16260 aggtctactg gtcgttgcca gacatgatgc aagaccccgt gaccttccgc tccacgcgcc 16320 agatcagcaa ctttccggtg gtgggcgccg agctgttgcc cgtgcactcc aagagcttct 16380 acaacgacca ggccgtctac tcccaactca tccgccagtt tacctctctg acccacgtgt 16440 tcaatcgctt tcccgagaac cagattttgg cgcgcccgcc agcccccacc atcaccaccg 16500 tcagtgaaaa cgttcctgct ctcacagatc acgggacgct accgctgcgc aacagcatcg 16560 gaggagtcca gcgagtgacc attactgacg ccagacgccg cacctgcccc tacgtttaca 16620 aggccctggg catagtctcg ccgcgcgtcc tatcgagccg cactttttga gcaagcatgt 16680 ccatccttat atcgcccagc aataacacag gctggggcct gcgcttccca agcaagatgt 16740 ttggcggggc caagaagcgc tccgaccaac acccagtgcg cgtgcgcggg cactaccgcg 16800 cgccctgggg cgcgcacaaa cgcggccgca ctgggcgcac caccgtcgat gacgccatcg 16860 acgcggtggt ggaggaggcg cgcaactaca cgcccacgcc gccaccagtg tccacagtgg 16920 acgcggccat tcagaccgtg gtgcgcggag cccggcgcta tgctaaaatg aagagacggc 16980 ggaggcgcgt agcacgtcgc caccgccgcc gacccggcac tgccgcccaa cgcgcggcgg 17040 cggccctgct taaccgcgca cgtcgcaccg gccgacgggc ggccatgcgg gccgctcgaa 17100 ggctggccgc gggtattgtc actgtgcccc ccaggtccag gcgacgagcg gccgccgcag 17160 cagccgcggc cattagtgct atgactcagg gtcgcagggg caacgtgtat tgggtgcgcg 17220 actcggttag cggcctgcgc gtgcccgtgc gcacccgccc cccgcgcaac tagattgcaa 17280 gaaaaaacta cttagactcg tactgttgta tgtatccagc ggcggcggcg cgcaacgaag 17340 ctatgtccaa gcgcaaaatc aaagaagaga tgctccaggt catcgcgccg gagatctatg 17400 gccccccgaa gaaggaagag caggattaca agccccgaaa gctaaagcgg gtcaaaaaga 17460 aaaagaaaga tgatgatgat gaacttgacg acgaggtgga actgctgcac gctaccgcgc 17520 ccaggcgacg ggtacagtgg aaaggtcgac gcgtaaaacg tgttttgcga cccggcacca 17580 ccgtagtctt tacgcccggt gagcgctcca cccgcaccta caagcgcgtg tatgatgagg 17640 tgtacggcga cgaggacctg cttgagcagg ccaacgagcg cctcggggag tttgcctacg 17700 gaaagcggca taaggacatg ctggcgttgc cgctggacga gggcaaccca acacctagcc 17760 taaagcccgt aacactgcag caggtgctgc ccgcgcttgc accgtccgaa gaaaagcgcg 17820 gcctaaagcg cgagtctggt gacttggcac ccaccgtgca gctgatggta cccaagcgcc 17880 agcgactgga agatgtcttg gaaaaaatga ccgtggaacc tgggctggag cccgaggtcc 17940 gcgtgcggcc aatcaagcag gtggcgccgg gactgggcgt gcagaccgtg gacgttcaga 18000 tacccactac cagtagcacc agtattgcca ccgccacaga gggcatggag acacaaacgt 18060 ccccggttgc ctcagcggtg gcggatgccg cggtgcaggc ggtcgctgcg gccgcgtcca 18120 agacctctac ggaggtgcaa acggacccgt ggatgtttcg cgtttcagcc ccccggcgcc 18180 cgcgcggttc gaggaagtac ggcgccgcca gcgcgctact gcccgaatat gccctacatc 18240 cttccattgc gcctaccccc ggctatcgtg gctacaccta ccgccccaga agacgagcaa 18300 ctacccgacg ccgaaccacc actggaaccc gccgccgccg tcgccgtcgc cagcccgtgc 18360 tggccccgat ttccgtgcgc agggtggctc gcgaaggagg caggaccctg gtgctgccaa 18420 cagcgcgcta ccaccccagc atcgtttaaa agccggtctt tgtggttctt gcagatatgg 18480 ccctcacctg ccgcctccgt ttcccggtgc cgggattccg aggaagaatg caccgtagga 18540 ggggcatggc cggccacggc ctgacgggcg gcatgcgtcg tgcgcaccac cggcggcggc 18600 gcgcgtcgca ccgtcgcatg cgcggcggta tcctgcccct ccttattcca ctgatcgccg 18660 cggcgattgg cgccgtgccc ggaattgcat ccgtggcctt gcaggcgcag agacactgat 18720 taaaaacaag ttgcatgtgg aaaaatcaaa ataaaaagtc tggactctca cgctcgcttg 18780 gtcctgtaac tattttgtag aatggaagac atcaactttg cgtctctggc cccgcgacac 18840 ggctcgcgcc cgttcatggg aaactggcaa gatatcggca ccagcaatat gagcggtggc 18900 gccttcagct ggggctcgct gtggagcggc attaaaaatt tcggttccac cgttaagaac 18960 tatggcagca aggcctggaa cagcagcaca ggccagatgc tgagggataa gttgaaagag 19020 caaaatttcc aacaaaaggt ggtagatggc ctggcctctg gcattagcgg ggtggtggac 19080 ctggccaacc aggcagtgca aaataagatt aacagtaagc ttgatccccg ccctcccgta 19140 gaggagcctc caccggccgt ggagacagtg tctccagagg ggcgtggcga aaagcgtccg 19200 cgccccgaca gggaagaaac tctggtgacg caaatagacg agcctccctc gtacgaggag 19260 gcactaaagc aaggcctgcc caccacccgt cccatcgcgc ccatggctac cggagtgctg 19320 ggccagcaca cacccgtaac gctggacctg cctccccccg ccgacaccca gcagaaacct 19380 gtgctgccag gcccgaccgc cgttgttgta acccgtccta gccgcgcgtc cctgcgccgc 19440 gccgccagcg gtccgcgatc gttgcggccc gtagccagtg gcaactggca aagcacactg 19500 aacagcatcg tgggtctggg ggtgcaatcc ctgaagcgcc gacgatgctt ctgaatagct 19560 aacgtgtcgt atgtgtgtca tgtatgcgtc catgtcgccg ccagaggagc tgctgagccg 19620 ccgcgcgccc gctttccaag atggctaccc cttcgatgat gccgcagtgg tcttacatgc 19680 acatctcggg ccaggacgcc tcggagtacc tgagccccgg gctggtgcag tttgcccgcg 19740 ccaccgagac gtacttcagc ctgaataaca agtttagaaa ccccacggtg gcgcctacgc 19800 acgacgtgac cacagaccgg tcccagcgtt tgacgctgcg gttcatccct gtggaccgtg 19860 aggatactgc gtactcgtac aaggcgcggt tcaccctagc tgtgggtgat aaccgtgtgc 19920 tggacatggc ttccacgtac tttgacatcc gcggcgtgct ggacaggggc cctactttta 19980 agccctactc tggcactgcc tacaacgccc tggctcccaa gggtgcccca aatccttgcg 20040 aatgggatga agctgctact gctcttgaaa taaacctaga agaagaggac gatgacaacg 20100 aagacgaagt agacgagcaa gctgagcagc aaaaaactca cgtatttggg caggcgcctt 20160 attctggtat aaatattaca aaggagggta ttcaaatagg tgtcgaaggt caaacaccta 20220 aatatgccga taaaacattt caacctgaac ctcaaatagg agaatctcag tggtacgaaa 20280 ctgaaattaa tcatgcagct gggagagtcc ttaaaaagac taccccaatg aaaccatgtt 20340 acggttcata tgcaaaaccc acaaatgaaa atggagggca aggcattctt gtaaagcaac 20400 aaaatggaaa gctagaaagt caagtggaaa tgcaattttt ctcaactact gaggcgaccg 20460 caggcaatgg tgataacttg actcctaaag tggtattgta cagtgaagat gtagatatag 20520 aaaccccaga cactcatatt tcttacatgc ccactattaa ggaaggtaac tcacgagaac 20580 taatgggcca acaatctatg cccaacaggc ctaattacat tgcttttagg gacaatttta 20640 ttggtctaat gtattacaac agcacgggta atatgggtgt tctggcgggc caagcatcgc 20700 agttgaatgc tgttgtagat ttgcaagaca gaaacacaga gctttcatac cagcttttgc 20760 ttgattccat tggtgataga accaggtact tttctatgtg gaatcaggct gttgacagct 20820 atgatccaga tgttagaatt attgaaaatc atggaactga agatgaactt ccaaattact 20880 gctttccact gggaggtgtg attaatacag agactcttac caaggtaaaa cctaaaacag 20940 gtcaggaaaa tggatgggaa aaagatgcta cagaattttc agataaaaat gaaataagag 21000 ttggaaataa ttttgccatg gaaatcaatc taaatgccaa cctgtggaga aatttcctgt 21060 actccaacat agcgctgtat ttgcccgaca agctaaagta cagtccttcc aacgtaaaaa 21120 tttctgataa cccaaacacc tacgactaca tgaacaagcg agtggtggct cccgggttag 21180 tggactgcta cattaacctt ggagcacgct ggtcccttga ctatatggac aacgtcaacc 21240 catttaacca ccaccgcaat gctggcctgc gctaccgctc aatgttgctg ggcaatggtc 21300 gctatgtgcc cttccacatc caggtgcctc agaagttctt tgccattaaa aacctccttc 21360 tcctgccggg ctcatacacc tacgagtgga acttcaggaa ggatgttaac atggttctgc 21420 agagctccct aggaaatgac ctaagggttg acggagccag cattaagttt gatagcattt 21480 gcctttacgc caccttcttc cccatggccc acaacaccgc ctccacgctt gaggccatgc 21540 ttagaaacga caccaacgac cagtccttta acgactatct ctccgccgcc aacatgctct 21600 accctatacc cgccaacgct accaacgtgc ccatatccat cccctcccgc aactgggcgg 21660 ctttccgcgg ctgggccttc acgcgcctta agactaagga aaccccatca ctgggctcgg 21720 gctacgaccc ttattacacc tactctggct ctatacccta cctagatgga accttttacc 21780 tcaaccacac ctttaagaag gtggccatta cctttgactc ttctgtcagc tggcctggca 21840 atgaccgcct gcttaccccc aacgagtttg aaattaagcg ctcagttgac ggggagggtt 21900 acaacgttgc ccagtgtaac atgaccaaag actggttcct ggtacaaatg ctagctaact 21960 acaacattgg ctaccagggc ttctatatcc cagagagcta caaggaccgc atgtactcct 22020 tctttagaaa cttccagccc atgagccgtc aggtggtgga tgatactaaa tacaaggact 22080 accaacaggt gggcatccta caccaacaca acaactctgg atttgttggc taccttgccc 22140 ccaccatgcg cgaaggacag gcctaccctg ctaacttccc ctatccgctt ataggcaaga 22200 ccgcagttga cagcattacc cagaaaaagt ttctttgcga tcgcaccctt tggcgcatcc 22260 cattctccag taactttatg tccatgggcg cactcacaga cctgggccaa aaccttctct 22320 acgccaactc cgcccacgcg ctagacatga cttttgaggt ggatcccatg gacgagccca 22380 cccttcttta tgttttgttt gaagtctttg acgtggtccg tgtgcaccgg ccgcaccgcg 22440 gcgtcatcga aaccgtgtac ctgcgcacgc ccttctcggc cggcaacgcc acaacataaa 22500 gaagcaagca acatcaacaa cagctgccgc catgggctcc agtgagcagg aactgaaagc 22560 cattgtcaaa gatcttggtt gtgggccata ttttttgggc acctatgaca agcgctttcc 22620 aggctttgtt tctccacaca agctcgcctg cgccatagtc aatacggccg gtcgcgagac 22680 tgggggcgta cactggatgg cctttgcctg gaacccgcac tcaaaaacat gctacctctt 22740 tgagcccttt ggcttttctg accagcgact caagcaggtt taccagtttg agtacgagtc 22800 actcctgcgc cgtagcgcca ttgcttcttc ccccgaccgc tgtataacgc tggaaaagtc 22860 cacccaaagc gtacaggggc ccaactcggc cgcctgtgga ctattctgct gcatgtttct 22920 ccacgccttt gccaactggc cccaaactcc catggatcac aaccccacca tgaaccttat 22980 taccggggta cccaactcca tgctcaacag tccccaggta cagcccaccc tgcgtcgcaa 23040 ccaggaacag ctctacagct tcctggagcg ccactcgccc tacttccgca gccacagtgc 23100 gcagattagg agcgccactt ctttttgtca cttgaaaaac atgtaaaaat aatgtactag 23160 agacactttc aataaaggca aatgctttta tttgtacact ctcgggtgat tatttacccc 23220 cacccttgcc gtctgcgccg tttaaaaatc aaaggggttc tgccgcgcat cgctatgcgc 23280 cactggcagg gacacgttgc gatactggtg tttagtgctc cacttaaact caggcacaac 23340 catccgcggc agctcggtga agttttcact ccacaggctg cgcaccatca ccaacgcgtt 23400 tagcaggtcg ggcgccgata tcttgaagtc gcagttgggg cctccgccct gcgcgcgcga 23460 gttgcgatac acagggttgc agcactggaa cactatcagc gccgggtggt gcacgctggc 23520 cagcacgctc ttgtcggaga tcagatccgc gtccaggtcc tccgcgttgc tcagggcgaa 23580 cggagtcaac tttggtagct gccttcccaa aaagggcgcg tgcccaggct ttgagttgca 23640 ctcgcaccgt agtggcatca aaaggtgacc gtgcccggtc tgggcgttag gatacagcgc 23700 ctgcataaaa gccttgatct gcttaaaagc cacctgagcc tttgcgcctt cagagaagaa 23760 catgccgcaa gacttgccgg aaaactgatt ggccggacag gccgcgtcgt gcacgcagca 23820 ccttgcgtcg gtgttggaga tctgcaccac atttcggccc caccggttct tcacgatctt 23880 ggccttgcta gactgctcct tcagcgcgcg ctgcccgttt tcgctcgtca catccatttc 23940 aatcacgtgc tccttattta tcataatgct tccgtgtaga cacttaagct cgccttcgat 24000 ctcagcgcag cggtgcagcc acaacgcgca gcccgtgggc tcgtgatgct tgtaggtcac 24060 ctctgcaaac gactgcaggt acgcctgcag gaatcgcccc atcatcgtca caaaggtctt 24120 gttgctggtg aaggtcagct gcaacccgcg gtgctcctcg ttcagccagg tcttgcatac 24180 ggccgccaga gcttccactt ggtcaggcag tagtttgaag ttcgccttta gatcgttatc 24240 cacgtggtac ttgtccatca gcgcgcgcgc agcctccatg cccttctccc acgcagacac 24300 gatcggcaca ctcagcgggt tcatcaccgt aatttcactt tccgcttcgc tgggctcttc 24360 ctcttcctct tgcgtccgca taccacgcgc cactgggtcg tcttcattca gccgccgcac 24420 tgtgcgctta cctcctttgc catgcttgat tagcaccggt gggttgctga aacccaccat 24480 ttgtagcgcc acatcttctc tttcttcctc gctgtccacg attacctctg gtgatggcgg 24540 gcgctcgggc ttgggagaag ggcgcttctt tttcttcttg ggcgcaatgg ccaaatccgc 24600 cgccgaggtc gatggccgcg ggctgggtgt gcgcggcacc agcgcgtctt gtgatgagtc 24660 ttcctcgtcc tcggactcga tacgccgcct catccgcttt tttgggggcg cccggggagg 24720 cggcggcgac ggggacgggg acgacacgtc ctccatggtt gggggacgtc gcgccgcacc 24780 gcgtccgcgc tcgggggtgg tttcgcgctg ctcctcttcc cgactggcca tttccttctc 24840 ctataggcag aaaaagatca tggagtcagt cgagaagaag gacagcctaa ccgccccctc 24900 tgagttcgcc accaccgcct ccaccgatgc cgccaacgcg cctaccacct tccccgtcga 24960 ggcacccccg cttgaggagg aggaagtgat tatcgagcag gacccaggtt ttgtaagcga 25020 agacgacgag gaccgctcag taccaacaga ggataaaaag caagaccagg acaacgcaga 25080 ggcaaacgag gaacaagtcg ggcgggggga cgaaaggcat ggcgactacc tagatgtggg 25140 agacgacgtg ctgttgaagc atctgcagcg ccagtgcgcc attatctgcg acgcgttgca 25200 agagcgcagc gatgtgcccc tcgccatagc ggatgtcagc cttgcctacg aacgccacct 25260 attctcaccg cgcgtacccc ccaaacgcca agaaaacggc acatgcgagc ccaacccgcg 25320 cctcaacttc taccccgtat ttgccgtgcc agaggtgctt gccacctatc acatcttttt 25380 ccaaaactgc aagatacccc tatcctgccg tgccaaccgc agccgagcgg acaagcagct 25440 ggccttgcgg cagggcgctg tcatacctga tatcgcctcg ctcaacgaag tgccaaaaat 25500 ctttgagggt cttggacgcg acgagaagcg cgcggcaaac gctctgcaac aggaaaacag 25560 cgaaaatgaa agtcactctg gagtgttggt ggaactcgag ggtgacaacg cgcgcctagc 25620 cgtactaaaa cgcagcatcg aggtcaccca ctttgcctac ccggcactta acctaccccc 25680 caaggtcatg agcacagtca tgagtgagct gatcgtgcgc cgtgcgcagc ccctggagag 25740 ggatgcaaat ttgcaagaac aaacagagga gggcctaccc gcagttggcg acgagcagct 25800 agcgcgctgg cttcaaacgc gcgagcctgc cgacttggag gagcgacgca aactaatgat 25860 ggccgcagtg ctcgttaccg tggagcttga gtgcatgcag cggttctttg ctgacccgga 25920 gatgcagcgc aagctagagg aaacattgca ctacaccttt cgacagggct acgtacgcca 25980 ggcctgcaag atctccaacg tggagctctg caacctggtc tcctaccttg gaattttgca 26040 cgaaaaccgc cttgggcaaa acgtgcttca ttccacgctc aagggcgagg cgcgccgcga 26100 ctacgtccgc gactgcgttt acttatttct atgctacacc tggcagacgg ccatgggcgt 26160 ttggcagcag tgcttggagg agtgcaacct caaggagctg cagaaactgc taaagcaaaa 26220 cttgaaggac ctatggacgg ccttcaacga gcgctccgtg gccgcgcacc tggcggacat 26280 cattttcccc gaacgcctgc ttaaaaccct gcaacagggt ctgccagact tcaccagtca 26340 aagcatgttg cagaacttta ggaactttat cctagagcgc tcaggaatct tgcccgccac 26400 ctgctgtgca cttcctagcg actttgtgcc cattaagtac cgcgaatgcc ctccgccgct 26460 ttggggccac tgctaccttc tgcagctagc caactacctt gcctaccact ctgacataat 26520 ggaagacgtg agcggtgacg gtctactgga gtgtcactgt cgctgcaacc tatgcacccc 26580 gcaccgctcc ctggtttgca attcgcagct gcttaacgaa agtcaaatta tcggtacctt 26640 tgagctgcag ggtccctcgc ctgacgaaaa gtccgcggct ccggggttga aactcactcc 26700 ggggctgtgg acgtcggctt accttcgcaa atttgtacct gaggactacc acgcccacga 26760 gattaggttc tacgaagacc aatcccgccc gccaaatgcg gagcttaccg cctgcgtcat 26820 tacccagggc cacattcttg gccaattgca agccatcaac aaagcccgcc aagagtttct 26880 gctacgaaag ggacgggggg tttacttgga cccccagtcc ggcgaggagc tcaacccaat 26940 ccccccgccg ccgcagccct atcagcagca gccgcgggcc cttgcttccc aggatggcac 27000 ccaaaaagaa gctgcagctg ccgccgccac ccacggacga ggaggaatac tgggacagtc 27060 aggcagagga ggttttggac gaggaggagg aggacatgat ggaagactgg gagagcctag 27120 acgaggaagc ttccgaggtc gaagaggtgt cagacgaaac accgtcaccc tcggtcgcat 27180 tcccctcgcc ggcgccccag aaatcggcaa ccggttccag catggctaca acctccgctc 27240 ctcaggcgcc gccggcactg cccgttcgcc gacccaaccg tagatgggac accactggaa 27300 ccagggccgg taagtccaag cagccgccgc cgttagccca agagcaacaa cagcgccaag 27360 gctaccgctc atggcgcggg cacaagaacg ccatagttgc ttgcttgcaa gactgtgggg 27420 gcaacatctc cttcgcccgc cgctttcttc tctaccatca cggcgtggcc ttcccccgta 27480 acatcctgca ttactaccgt catctctaca gcccatactg caccggcggc agcggcagcg 27540 gcagcaacag cagcggccac acagaagcaa aggcgaccgg atagcaagac tctgacaaag 27600 cccaagaaat ccacagcggc ggcagcagca ggaggaggag cgctgcgtct ggcgcccaac 27660 gaacccgtat cgacccgcga gcttagaaac aggatttttc ccactctgta tgctatattt 27720 caacagagca ggggccaaga acaagagctg aaaataaaaa acaggtctct gcgatccctc 27780 acccgcagct gcctgtatca caaaagcgaa gatcagcttc ggcgcacgct ggaagacgcg 27840 gaggctctct tcagtaaata ctgcgcgctg actcttaagg actagtttcg cgccctttct 27900 caaatttaag cgcgaaaact acgtcatctc cagcggccac acccggcgcc agcacctgtc 27960 gtcagcgcca tttcaacttt gtatacaaaa gttgtgatga gcaaggaaat tcccacgccc 28020 tacatgtgga gttaccagcc acaaatggga cttgcggctg gagctgccca agactactca 28080 acccgaataa actacatgag cgcgggaccc cacatgatat cccgggtcaa cggaatacgc 28140 gcccaccgaa accgaattct cctggaacag gcggctatta ccaccacacc tcgtaataac 28200 cttaatcccc gtagttggcc cgctgccctg gtgtaccagg aaagtcccgc tcccaccact 28260 gtggtacttc ccagagacgc ccaggccgaa gttcagatga ctaactcagg ggcgcagctt 28320 gcgggcggct ttcgtcacag ggtgcggtcg cccgggcagg gtataactca cctgacaatc 28380 agagggcgag gtattcagct caacgacgag tcggtgagct cctcgcttgg tctccgtccg 28440 gacgggacat ttcagatcgg cggcgccggc cgctcttcat tcacgcctcg tcaggcaatc 28500 ctaactctgc agacctcgtc ctctgagccg cgctctggag gcattggaac tctgcaattt 28560 attgaggagt ttgtgccatc ggtctacttt aaccccttct cgggacctcc cggccactat 28620 ccggatcaat ttattcctaa ctttgacgcg gtaaaggact cggcggacgg ctacgactga 28680 atgttaagtg gagaggcaga gcaactgcgc ctgaaacacc tggtccactg tcgccgccac 28740 aagtgctttg cccgcgactc cggtgagttt tgctactttg aattgcccga ggatcatatc 28800 gagggcccgg cgcacggcgt ccggcttacc gcccagggag agcttgcccg tagcctgatt 28860 cgggagttta cccagcgccc cctgctagtt gagcgggaca ggggaccctg tgttctcact 28920 gtgatttgca actgtcctaa ccctggatta catcaagatc tttgttgcca tctctgtgct 28980 gagtataata aatacagaaa ttaaaatata ctggggctcc tatcgccatc ctgtaaacgc 29040 caccgtcttc acccgcccaa gcaaaccaag gcgaacctta cctggtactt ttaacatctc 29100 tccctctgtg atttacaaca gtttcaaccc agacggagtg agtctacgag agaacctctc 29160 cgagctcagc tactccatca gaaaaaacac caccctcctt acctgccggg aacgtacgag 29220 tgcgtcaccg gccgctgcac cacacctacc gcctgaccgt aaaccagact ttttccggac 29280 agacctcaat aactctgttt accagaacag gaggtgagct tagaaaaccc ttagggtatt 29340 aggccaaagg cgcagctact gtggggttta tgaacaattc aagcaactct acgggctatt 29400 ctaattcagg tttctctaga atcggggttg gggttattct ctgtcttgtg attctcttta 29460 ttcttatact aacgcttctc tgcctaaggc tcgccgcctg ctgtgtgcac atttgcattt 29520 attgtcagct ttttaaacgc tggggtcgcc acccaagatg attaggtaca taatcctagg 29580 tttactcacc cttgcgtcag cccacggtac cacccaaaag gtggatttta aggagccagc 29640 ctgtaatgtt acattcgcag ctgaagctaa tgagtgcacc actcttataa aatgcaccac 29700 agaacatgaa aagctgctta ttcgccacaa aaacaaaatt ggcaagtatg ctgtttatgc 29760 tatttggcag ccaggtgaca ctacagagta taatgttaca gttttccagg gtaaaagtca 29820 taaaactttt atgtatactt ttccatttta tgaaatgtgc gacattacca tgtacatgag 29880 caaacagtat aagttgtggc ccccacaaaa ttgtgtggaa aacactggca ctttctgctg 29940 cactgctatg ctaattacag tgctcgcttt ggtctgtacc ctactctata ttaaatacaa 30000 aagcagacgc agctttattg aggaaaagaa aatgccttaa tttactaagt tacaaagcta 30060 atgtcaccac taactgcttt actcgctgct tgcaaaacaa attcaaaaag ttagcattat 30120 aattagaata ggatttaaac cccccggtca tttcctgctc aataccattc ccctgaacaa 30180 ttgactctat gtgggatatg ctccagcgct acaaccttga agtcaggctt cctggatgtc 30240 agcatctgac tttggccagc acctgtcccg cggatttgtt ccagtccaac tacagcgacc 30300 caccctaaca gagatgacca acacaaccaa cgcggccgcc gctaccggac ttacatctac 30360 cacaaataca ccccaagttt ctgcctttgt caataactgg gataacttgg gcatgtggtg 30420 gttctccata gcgcttatgt ttgtatgcct tattattatg tggctcatct gctgcctaaa 30480 gcgcaaacgc gcccgaccac ccatctatag tcccatcatt gtgctacacc caaacaatga 30540 tggaatccat agattggacg gactgaaaca catgttcttt tctcttacag tatgattaaa 30600 tgagacatga ttcctcgagt ttttatatta ctgacccttg ttgcgctttt ttgtgcgtgc 30660 tccacattgg ctgcggtttc tcacatcgaa gtagactgca ttccagcctt cacagtctat 30720 ttgctttacg gatttgtcac cctcacgctc atctgcagcc tcatcactgt ggtcatcgcc 30780 tttatccagt gcattgactg ggtctgtgtg cgctttgcat atctcagaca ccatccccag 30840 tacagggaca ggactatagc tgagcttctt agaattcttt aattatgaaa tttactgtga 30900 cttttctgct gattatttgc accctatctg cgttttgttc cccgacctcc aagcctcaaa 30960 gacatatatc atgcagattc actcgtatat ggaatattcc aagttgctac aatgaaaaaa 31020 gcgatctttc cgaagcctgg ttatatgcaa tcatctctgt tatggtgttc tgcagtacca 31080 tcttagccct agctatatat ccctaccttg acattggctg gaacgcaata gatgccatga 31140 accacccaac tttccccgcg cccgctatgc ttccactgca acaagttgtt gccggcggct 31200 ttgtcccagc caatcagcct cgcccacctt ctcccacccc cactgaaatc agctacttta 31260 atctaacagg aggagatgac tgacacccta gatctagaaa tggacggaat tattacagag 31320 cagcgcctgc tagaaagacg cagggcagcg gccgagcaac agcgcatgaa tcaagagctc 31380 caagacatgg ttaacttgca ccagtgcaaa aggggtatct tttgtctggt aaagcaggcc 31440 aaagtcacct acgacagtaa taccaccgga caccgcctta gctacaagtt gccaaccaag 31500 cgtcagaaat tggtggtcat ggtgggagaa aagcccatta ccataactca gcactcggta 31560 gaaaccgaag gctgcattca ctcaccttgt caaggacctg aggatctctg cacccttatt 31620 aagaccctgt gcggtctcaa agatcttatt ccctttaact aataaaaaaa aataataaag 31680 catcacttac ttaaaatcag ttagcaaatt tctgtccagt ttattcagca gcacctcctt 31740 gccctcctcc cagctctggt attgcagctt cctcctggct gcaaactttc tccacaatct 31800 aaatggaatg tcagtttcct cctgttcctg tccatccgca cccactatct tcatgttgtt 31860 gcagatgaag cgcgcaagac cgtctgaaga taccttcaac cccgtgtatc catatgacac 31920 ggaaaccggt cctccaactg tgccttttct tactcctccc tttgtatccc ccaatgggtt 31980 tcaagagagt ccccctgggg tactctcttt gcgcctatcc gaacctctag ttacctccaa 32040 tggcatgctt gcgctcaaaa tgggcaacgg cctctctctg gacgaggccg gcaaccttac 32100 ctcccaaaat gtaaccactg tgagcccacc tctcaaaaaa accaagtcaa acataaacct 32160 ggaaatatct gcacccctca cagttacctc agaagcccta actgtggctg ccgccgcacc 32220 tctaatggtc gcgggcaaca cactcaccat gcaatcacag gccccgctaa ccgtgcacga 32280 ctccaaactt agcattgcca cccaaggacc cctcacagtg tcagaaggaa agctagccct 32340 gcaaacatca ggccccctca ccaccaccga tagcagtacc cttactatca ctgcctcacc 32400 ccctctaact actgccactg gtagcttggg cattgacttg aaagagccca tttatacaca 32460 aaatggaaaa ctaggactaa agtacggggc tcctttgcat gtaacagacg acctaaacac 32520 tttgaccgta gcaactggtc caggtgtgac tattaataat acttccttgc aaactaaagt 32580 tactggagcc ttgggttttg attcacaagg caatatgcaa cttaatgtag caggaggact 32640 aaggattgat tctcaaaaca gacgccttat acttgatgtt agttatccgt ttgatgctca 32700 aaaccaacta aatctaagac taggacaggg ccctcttttt ataaactcag cccacaactt 32760 ggatattaac tacaacaaag gcctttactt gtttacagct tcaaacaatt ccaaaaagct 32820 tgaggttaac ctaagcactg ccaaggggtt gatgtttgac gctacagcca tagccattaa 32880 tgcaggagat gggcttgaat ttggttcacc taatgcacca aacacaaatc ccctcaaaac 32940 aaaaattggc catggcctag aatttgattc aaacaaggct atggttccta aactaggaac 33000 tggccttagt tttgacagca caggtgccat tacagtagga aacaaaaata atgataagct 33060 aactttgtgg accacaccag ctccatctcc taactgtaga ctaaatgcag agaaagatgc 33120 taaactcact ttggtcttaa caaaatgtgg cagtcaaata cttgctacag tttcagtttt 33180 ggctgttaaa ggcagtttgg ctccaatatc tggaacagtt caaagtgctc atcttattat 33240 aagatttgac gaaaatggag tgctactaaa caattccttc ctggacccag aatattggaa 33300 ctttagaaat ggagatctta ctgaaggcac agcctataca aacgctgttg gatttatgcc 33360 taacctatca gcttatccaa aatctcacgg taaaactgcc aaaagtaaca ttgtcagtca 33420 agtttactta aacggagaca aaactaaacc tgtaacacta accattacac taaacggtac 33480 acaggaaaca ggagacacaa ctccaagtgc atactctatg tcattttcat gggactggtc 33540 tggccacaac tacattaatg aaatatttgc cacatcctct tacacttttt catacattgc 33600 ccaagaataa agaatcgttt gtgttatgtt tcaacgtgtt tatttttcaa ttgcagaaaa 33660 tttcaagtca tttttcattc agtagtatag ccccaccacc acatagctta tacagatcac 33720 cgtacctcaa ctttgtataa taaagttgta atcaaactca cagaacccta gtattcaacc 33780 tgccacctcc ctcccaacac acagagtaca cagtcctttc tccccggctg gccttaaaaa 33840 gcatcatatc atgggtaaca gacatattct taggtgttat attccacacg gtttcctgtc 33900 gagccaaacg ctcatcagtg atattaataa actccccggg cagctcactt aagttcatgt 33960 cgctgtccag ctgctgagcc acaggctgct gtccaacttg cggttgctta acgggcggcg 34020 aaggagaagt ccacgcctac atgggggtag agtcataatc gtgcatcagg atagggcggt 34080 ggtgctgcag cagcgcgcga ataaactgct gccgccgccg ctccgtcctg caggaataca 34140 acatggcagt ggtctcctca gcgatgattc gcaccgcccg cagcataagg cgccttgtcc 34200 tccgggcaca gcagcgcacc ctgatctcac ttaaatcagc acagtaactg cagcacagca 34260 ccacaatatt gttcaaaatc ccacagtgca aggcgctgta tccaaagctc atggcgggga 34320 ccacagaacc cacgtggcca tcataccaca agcgcaggta gattaagtgg cgacccctca 34380 taaacacgct ggacataaac attacctctt ttggcatgtt gtaattcacc acctcccggt 34440 accatataaa cctctgatta aacatggcgc catccaccac catcctaaac cagctggcca 34500 aaacctgccc gccggctata cactgcaggg aaccgggact ggaacaatga cagtggagag 34560 cccaggactc gtaaccatgg atcatcatgc tcgtcatgat atcaatgttg gcacaacaca 34620 ggcacacgtg catacacttc ctcaggatta caagctcctc ccgcgttaga accatatccc 34680 agggaacaac ccattcctga atcagcgtaa atcccacact gcagggaaga cctcgcacgt 34740 aactcacgtt gtgcattgtc aaagtgttac attcgggcag cagcggatga tcctccagta 34800 tggtagcgcg ggtttctgtc tcaaaaggag gtagacgatc cctactgtac ggagtgcgcc 34860 gagacaaccg agatcgtgtt ggtcgtagtg tcatgccaaa tggaacgccg gacgtagtca 34920 tatttcctga agcaaaacca ggtgcgggcg tgacaaacag atctgcgtct ccggtctcgc 34980 cgcttagatc gctctgtgta gtagttgtag tatatccact ctctcaaagc atccaggcgc 35040 cccctggctt cgggttctat gtaaactcct tcatgcgccg ctgccctgat aacatccacc 35100 accgcagaat aagccacacc cagccaacct acacattcgt tctgcgagtc acacacggga 35160 ggagcgggaa gagctggaag aaccatgttt ttttttttat tccaaaagat tatccaaaac 35220 ctcaaaatga agatctatta agtgaacgcg ctcccctccg gtggcgtggt caaactctac 35280 agccaaagaa cagataatgg catttgtaag atgttgcaca atggcttcca aaaggcaaac 35340 ggccctcacg tccaagtgga cgtaaaggct aaacccttca gggtgaatct cctctataaa 35400 cattccagca ccttcaacca tgcccaaata attctcatct cgccaccttc tcaatatatc 35460 tctaagcaaa tcccgaatat taagtccggc cattgtaaaa atctgctcca gagcgccctc 35520 caccttcagc ctcaagcagc gaatcatgat tgcaaaaatt caggttcctc acagacctgt 35580 ataagattca aaagcggaac attaacaaaa ataccgcgat cccgtaggtc ccttcgcagg 35640 gccagctgaa cataatcgtg caggtctgca cggaccagcg cggccacttc cccgccagga 35700 accatgacaa aagaacccac actgattatg acacgcatac tcggagctat gctaaccagc 35760 gtagccccga tgtaagcttg ttgcatgggc ggcgatataa aatgcaaggt gctgctcaaa 35820 aaatcaggca aagcctcgcg caaaaaagaa agcacatcgt agtcatgctc atgcagataa 35880 aggcaggtaa gctccggaac caccacagaa aaagacacca tttttctctc aaacatgtct 35940 gcgggtttct gcataaacac aaaataaaat aacaaaaaaa catttaaaca ttagaagcct 36000 gtcttacaac aggaaaaaca acccttataa gcataagacg gactacggcc atgccggcgt 36060 gaccgtaaaa aaactggtca ccgtgattaa aaagcaccac cgacagctcc tcggtcatgt 36120 ccggagtcat aatgtaagac tcggtaaaca catcaggttg attcacatcg gtcagtgcta 36180 aaaagcgacc gaaatagccc gggggaatac atacccgcag gcgtagagac aacattacag 36240 cccccatagg aggtataaca aaattaatag gagagaaaaa cacataaaca cctgaaaaac 36300 cctcctgcct aggcaaaata gcaccctccc gctccagaac aacatacagc gcttccacag 36360 cggcagccat aacagtcagc cttaccagta aaaaagaaaa cctattaaaa aaacaccact 36420 cgacacggca ccagctcaat cagtcacagt gtaaaaaagg gccaagtgca gagcgagtat 36480 atataggact aaaaaatgac gtaacggtta aagtccacaa aaaacaccca gaaaaccgca 36540 cgcgaaccta cgcccagaaa cgaaagccaa aaaacccaca acttcctcaa atcgtcactt 36600 ccgttttccc acgttacgtc acttcccatt ttaagaaaac tacaattccc aacacataca 36660 agttactccg ccctaaaacc tacgtcaccc gccccgttcc cacgccccgc gccacgtcac 36720 aaactccacc ccctcattat catattggct tcaatccaaa ataaggtata ttattgatga 36780 tg 36782 <210> 5 <211> 36794 <212> DNA <213> Artificial Sequence <220> <223> Synthetic adenovirus genome YPet-P2A-(DNA Poly) <400> 5 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420 cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480 tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540 tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600 aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660 tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720 cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780 gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840 cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900 ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960 cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020 caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080 ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140 tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200 gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260 ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320 cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380 ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440 gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500 cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560 ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620 gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680 cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740 ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800 tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860 gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920 caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980 gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040 agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100 aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160 cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220 gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280 gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340 gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400 gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460 tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520 ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580 tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640 agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700 tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760 gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820 acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880 gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940 aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000 ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060 gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120 tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180 acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240 aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300 tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360 gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420 tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480 ttggctctag cgatgaagat acagattgag gtactgaaat gtgtgggcgt ggcttaaggg 3540 tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg 3600 ccgccgccca acttttctat acaaagttgt aatgagcacc aactcgtttg atggaagcat 3660 tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat 3720 gggctccagc attgatggtc gccccgtcct gcccgcaaac tctactacct tgacctacga 3780 gaccgtgtct ggaacgccgt tggagactgc agcctccgcc gccgcttcag ccgctgcagc 3840 caccgcccgc gggattgtga ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc 3900 ttcccgttca tccgcccgcg atgacaagtt gacggctctt ttggcacaat tggattcttt 3960 gacccgggaa cttaatgtcg tttctcagca gctgttggat ctgcgccagc aggtttctgc 4020 cctgaaggct tcctcccctc ccaatgcggt ttaaaacata aataaaaaac cagactctgt 4080 ttggatttgg atcaagcata agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg 4140 taggcccggg accagcggtc tcggtcgttg agggtcctgt gtattttttc caggacgtgg 4200 taaaggtgac tctggatgtt cagatacatg ggcataagcc cgtctctggg gtggaggtag 4260 caccactgca gagcttcatg ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag 4320 cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc tgattgccag gggcaggccc 4380 ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt gcatacgtgg ggatatgaga 4440 tgcatcttgg actgtatttt taggttggct atgttcccag ccatatccct ccggggattc 4500 atgttgtgca gaaccaccag cacagtgtat ccggtgcact tgggaaattt gtcatgtagc 4560 ttagaaggaa atgcgtggaa gaacttggag acgcccttgt gacctccaag attttccatg 4620 cattcgtcca taatgatggc aatgggccca cgggcggcgg cctgggcgaa gatatttctg 4680 ggatcactaa cgtcatagtt gtgttccagg atgagatcgt cataggccat ttttacaaag 4740 cgcgggcgga gggtgccaga ctgcggtata atggttccat ccggcccagg ggcgtagtta 4800 ccctcacaga tttgcatttc ccacgctttg agttcagatg gggggatcat gtctacctgc 4860 ggggcgatga agaaaacggt ttccggggta ggggagatca gctgggaaga aagcaggttc 4920 ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa tcacacctat taccgggtgc 4980 aactggtagt taagagagct gcagctgccg tcatccctga gcaggggggc cacttcgtta 5040 agcatgtccc tgactcgcat gttttccctg accaaatccg ccagaaggcg ctcgccgccc 5100 agcgatagca gttcttgcaa ggaagcaaag tttttcaacg gtttgagacc gtccgccgta 5160 ggcatgcttt tgagcgtttg accaagcagt tccaggcggt cccacagctc ggtcacctgc 5220 tctacggcat ctcgatccag catatctcct cgtttcgcgg gttggggcgg ctttcgctgt 5280 acggcagtag tcggtgctcg tccagacggg ccagggtcat gtctttccac gggcgcaggg 5340 tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc tccgggctgc gcgctggcca 5400 gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt 5460 cggccaggta gcatttgacc atggtgtcat agtccagccc ctccgcggcg tggcccttgg 5520 cgcgcagctt gcccttggag gaggcgccgc acgaggggca gtgcagactt ttgagggcgt 5580 agagcttggg cgcgagaaat accgattccg gggagtaggc atccgcgccg caggccccgc 5640 agacggtctc gcattccacg agccaggtga gctctggccg ttcggggtca aaaaccaggt 5700 ttcccccatg ctttttgatg cgtttcttac ctctggtttc catgagccgg tgtccacgct 5760 cggtgacgaa aaggctgtcc gtgtccccgt atacagactt gagaggcctg tcctcgagcg 5820 gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacaaag gctcgcgtcc 5880 aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact agggggtcca 5940 ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag gtgattggtt 6000 tgtaggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag ggggtggggg 6060 cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt tggggtgagt 6120 actccctctg aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg 6180 aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgca tccatctggt 6240 cagaaaagac aatctttttg ttgtcaagct tggtggcaaa cgacccgtag agggcgttgg 6300 acagcaactt ggcgatggag cgcagggttt ggtttttgtc gcgatcggcg cgctccttgg 6360 ccgcgatgtt tagctgcacg tattcgcgcg caacgcaccg ccattcggga aagacggtgg 6420 tgcgctcgtc gggcaccagg tgcacgcgcc aaccgcggtt gtgcagggtg acaaggtcaa 6480 cgctggtggc tacctctccg cgtaggcgct cgttggtcca gcagaggcgg ccgcccttgc 6540 gcgagcagaa tggcggtagg gggtctagct gcgtctcgtc cggggggtct gcgtccacgg 6600 taaagacccc gggcagcagg cgcgcgtcga agtagtctat cttgcatcct tgcaagtcta 6660 gcgcctgctg ccatgcgcgg gcggcaagcg cgcgctcgta tgggttgagt gggggacccc 6720 atggcatggg gtgggtgagc gcggaggcgt acatgccgca aatgtcgtaa acgtagaggg 6780 gctctctgag tattccaaga tatgtagggt agcatcttcc accgcggatg ctggcgcgca 6840 cgtaatcgta tagttcgtgc gagggagcga ggaggtcggg accgaggttg ctacgggcgg 6900 gctgctctgc tcggaagact atctgcctga agatggcatg tgagttggat gatatggttg 6960 gacgctggaa gacgttgaag ctggcgtctg tgagacctac cgcgtcacgc acgaaggagg 7020 cgtaggagtc gcgcagcttg ttgaccagct cggcggtgac ctgcacgtct agggcgcagt 7080 agtccagggt ttccttgatg atgtcatact tatcctgtcc cttttttttc cacagctcgc 7140 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct 7200 ccgaacggta agagcctagc atgtagaact ggttgacggc ctggtaggcg cagcatccct 7260 tttctacggg tagcgcgtat gcctgcgcgg ccttccggag cgaggtgtgg gtgagcgcaa 7320 aggtgtccct gaccatgact ttgaggtact ggtatttgaa gtcagtgtcg tcgcatccgc 7380 cctgctccca gagcaaaaag tccgtgcgct ttttggaacg cggatttggc agggcgaagg 7440 tgacatcgtt gaagagtatc tttcccgcgc gaggcataaa gttgcgtgtg atgcggaagg 7500 gtcccggcac ctcggaacgg ttgttaatta cctgggcggc gagcacgatc tcgtcaaagc 7560 cgttgatgtt gtggcccaca atgtaaagtt ccaagaagcg cgggatgccc ttgatggaag 7620 gcaatttttt aagttcctcg taggtgagct cttcagggga gctgagcccg tgctctgaaa 7680 gggcccagtc tgcaagatga gggttggaag cgacgaatga gctccacagg tcacgggcca 7740 ttagcatttg caggtggtcg cgaaaggtcc taaactggcg acctatggcc attttttctg 7800 gggtgatgca gtagaaggta agcgggtctt gttcccagcg gtcccatcca aggttcgcgg 7860 ctaggtctcg cgcggcagtc actagaggct catctccgcc gaacttcatg accagcatga 7920 agggcacgag ctgcttccca aaggccccca tccaagtata ggtctctaca tcgtaggtga 7980 caaagagacg ctcggtgcga ggatgcgagc cgatcgggaa gaactggatc tcccgccacc 8040 aattggagga gtggctattg atgtggtgaa agtagaagtc cctgcgacgg gccgaacact 8100 cgtgctggct tttgtaaaaa cgtgcgcagt actggcagcg gtgcacgggc tgtacatcct 8160 gcacgaggtt gacctgacga ccgcgcacaa ggaagcagag tgggaatttg agcccctcgc 8220 ctggcgggtt tggctggtgg tcttctactt cggctgcttg tccttgaccg tctggctgct 8280 cgaggggagt tacggtggat cggaccacca cgccgcgcga gcccaaagtc cagatgtccg 8340 cgcgcggcgg tcggagcttg atgacaacat cgcgcagatg ggagctgtcc atggtctgga 8400 gctcccgcgg cgtcaggtca ggcgggagct cctgcaggtt tacctcgcat agacgggtca 8460 gggcgcgggc tagatccagg tgatacctaa tttccagggg ctggttggtg gcggcgtcga 8520 tggcttgcaa gaggccgcat ccccgcggcg cgactacggt accgcgcggc gggcggtggg 8580 ccgcgggggt gtccttggat gatgcatcta aaagcggtga cgcgggcgag cccccggagg 8640 tagggggggc tccggacccg ccgggagagg gggcaggggc acgtcggcgc cgcgcgcggg 8700 caggagctgg tgctgcgcgc gtaggttgct ggcgaacgcg acgacgcggc ggttgatctc 8760 ctgaatctgg cgcctctgcg tgaagacgac gggcccggtg agcttgagcc tgaaagagag 8820 ttcgacagaa tcaatttcgg tgtcgttgac ggcggcctgg cgcaaaatct cctgcacgtc 8880 tcctgagttg tcttgatagg cgatctcggc catgaactgc tcgatctctt cctcctggag 8940 atctccgcgt ccggctcgct ccacggtggc ggcgaggtcg ttggaaatgc gggccatgag 9000 ctgcgagaag gcgttgaggc ctccctcgtt ccagacgcgg ctgtagacca cgcccccttc 9060 ggcatcgcgg gcgcgcatga ccacctgcgc gagattgagc tccacgtgcc gggcgaagac 9120 ggcgtagttt cgcaggcgct gaaagaggta gttgagggtg gtggcggtgt gttctgccac 9180 gaagaagtac ataacccagc gtcgcaacgt ggattcgttg atatccccca aggcctcaag 9240 gcgctccatg gcctcgtaga agtccacggc gaagttgaaa aactgggagt tgcgcgccga 9300 cacggttaac tcctcctcca gaagacggat gagctcggcg acagtgtcgc gcacctcgcg 9360 ctcaaaggct acaggggcct cttcttcttc ttcaatctcc tcttccataa gggcctcccc 9420 ttcttcttct tctggcggcg gtgggggagg ggggacacgg cggcgacgac ggcgcaccgg 9480 gaggcggtcg acaaagcgct cgatcatctc cccgcggcga cggcgcatgg tctcggtgac 9540 ggcgcggccg ttctcgcggg ggcgcagttg gaagacgccg cccgtcatgt cccggttatg 9600 ggttggcggg gggctgccat gcggcaggga tacggcgcta acgatgcatc tcaacaattg 9660 ttgtgtaggt actccgccgc cgagggacct gagcgagtcc gcatcgaccg gatcggaaaa 9720 cctctcgaga aaggcgtcta accagtcaca gtcgcaaggt aggctgagca ccgtggcggg 9780 cggcagcggg cggcggtcgg ggttgtttct ggcggaggtg ctgctgatga tgtaattaaa 9840 gtaggcggtc ttgagacggc ggatggtcga cagaagcacc atgtccttgg gtccggcctg 9900 ctgaatgcgc aggcggtcgg ccatgcccca ggcttcgttt tgacatcggc gcaggtcttt 9960 gtagtagtct tgcatgagcc tttctaccgg cacttcttct tctccttcct cttgtcctgc 10020 atctcttgca tctatcgctg cggcggcggc ggagtttggc cgtaggtggc gccctcttcc 10080 tcccatgcgt gtgaccccga agcccctcat cggctgaagc agggctaggt cggcgacaac 10140 gcgctcggct aatatggcct gctgcacctg cgtgagggta gactggaagt catccatgtc 10200 cacaaagcgg tggtatgcgc ccgtgttgat ggtgtaagtg cagttggcca taacggacca 10260 gttaacggtc tggtgacccg gctgcgagag ctcggtgtac ctgagacgcg agtaagccct 10320 cgagtcaaat acgtagtcgt tgcaagtccg caccaggtac tggtatccca ccaaaaagtg 10380 cggcggcggc tggcggtaga ggggccagcg tagggtggcc ggggctccgg gggcgagatc 10440 ttccaacata aggcgatgat atccgtagat gtacctggac atccaggtga tgccggcggc 10500 ggtggtggag gcgcgcggaa agtcgcggac gcggttccag atgttgcgca gcggcaaaaa 10560 gtgctccatg gtcgggacgc tctggccggt caggcgcgcg caatcgttga cgctctagac 10620 cgtgcaaaag gagagcctgt aagcgggcac tcttccgtgg tctggtggat aaattcgcaa 10680 gggtatcatg gcggacgacc ggggttcgag ccccgtatcc ggccgtccgc cgtgatccat 10740 gcggttaccg cccgcgtgtc gaacccaggt gtgcgacgtc agacaacggg ggagtgctcc 10800 ttttggcttc cttccaggcg cggcggctgc tgcgctagct tttttggcca ctggccgcgc 10860 gcagcgtaag cggttaggct ggaaagcgaa agcattaagt ggctcgctcc ctgtagccgg 10920 agggttattt tccaagggtt gagtcgcggg acccccggtt cgagtctcgg accggccgga 10980 ctgcggcgaa cgggggtttg cctccccgtc atgcaagacc ccgcttgcaa attcctccgg 11040 aaacagggac gagccccttt tttgcttttc ccagatgcat ccggtgctgc ggcagatgcg 11100 cccccctcct cagcagcggc aagagcaaga gcagcggcag acatgcaggg caccctcccc 11160 tcctcctacc gcgtcaggag gggcgacatc cgcggttgac gcggcagcag atggtgatta 11220 cgaacccccg cggcgccggg cccggcacta cctggacttg gaggagggcg agggcctggc 11280 gcggctagga gcgccctctc ctgagcggca cccaagggtg cagctgaagc gtgatacgcg 11340 tgaggcgtac gtgccgcggc agaacctgtt tcgcgaccgc gagggagagg agcccgagga 11400 gatgcgggat cgaaagttcc acgcagggcg cgagctgcgg catggcctga atcgcgagcg 11460 gttgctgcgc gaggaggact ttgagcccga cgcgcgaacc gggattagtc ccgcgcgcgc 11520 acacgtggcg gccgccgacc tggtaaccgc atacgagcag acggtgaacc aggagattaa 11580 ctttcaaaaa agctttaaca accacgtgcg tacgcttgtg gcgcgcgagg aggtggctat 11640 aggactgatg catctgtggg actttgtaag cgcgctggag caaaacccaa atagcaagcc 11700 gctcatggcg cagctgttcc ttatagtgca gcacagcagg gacaacgagg cattcaggga 11760 tgcgctgcta aacatagtag agcccgaggg ccgctggctg ctcgatttga taaacatcct 11820 gcagagcata gtggtgcagg agcgcagctt gagcctggct gacaaggtgg ccgccatcaa 11880 ctattccatg cttagcctgg gcaagtttta cgcccgcaag atataccata ccccttacgt 11940 tcccatagac aaggaggtaa agatcgaggg gttctacatg cgcatggcgc tgaaggtgct 12000 taccttgagc gacgacctgg gcgtttatcg caacgagcgc atccacaagg ccgtgagcgt 12060 gagccggcgg cgcgagctca gcgaccgcga gctgatgcac agcctgcaaa gggccctggc 12120 tggcacgggc agcggcgata gagaggccga gtcctacttt gacgcgggcg ctgacctgcg 12180 ctgggcccca agccgacgcg ccctggaggc agctggggcc ggacctgggc tggcggtggc 12240 acccgcgcgc gctggcaacg tcggcggcgt ggaggaatat gacgaggacg atgagtacga 12300 gccagaggac ggcgagtact aagcggtgat gtttctgatc agatgatgca agacgcaacg 12360 gacccggcgg tgcgggcggc gctgcagagc cagccgtccg gccttaactc cacggacgac 12420 tggcgccagg tcatggaccg catcatgtcg ctgactgcgc gcaatcctga cgcgttccgg 12480 cagcagccgc aggccaaccg gctctccgca attctggaag cggtggtccc ggcgcgcgca 12540 aaccccacgc acgagaaggt gctggcgatc gtaaacgcgc tggccgaaaa cagggccatc 12600 cggcccgacg aggccggcct ggtctacgac gcgctgcttc agcgcgtggc tcgttacaac 12660 agcggcaacg tgcagaccaa cctggaccgg ctggtggggg atgtgcgcga ggccgtggcg 12720 cagcgtgagc gcgcgcagca gcagggcaac ctgggctcca tggttgcact aaacgccttc 12780 ctgagtacac agcccgccaa cgtgccgcgg ggacaggagg actacaccaa ctttgtgagc 12840 gcactgcggc taatggtgac tgagacaccg caaagtgagg tgtaccagtc tgggccagac 12900 tattttttcc agaccagtag acaaggcctg cagaccgtaa acctgagcca ggctttcaaa 12960 aacttgcagg ggctgtgggg ggtgcgggct cccacaggcg accgcgcgac cgtgtctagc 13020 ttgctgacgc ccaactcgcg cctgttgctg ctgctaatag cgcccttcac ggacagtggc 13080 agcgtgtccc gggacacata cctaggtcac ttgctgacac tgtaccgcga ggccataggt 13140 caggcgcatg tggacgagca tactttccag gagattacaa gtgtcagccg cgcgctgggg 13200 caggaggaca cgggcagcct ggaggcaacc ctaaactacc tgctgaccaa ccggcggcag 13260 aagatcccct cgttgcacag tttaaacagc gaggaggagc gcattttgcg ctacgtgcag 13320 cagagcgtga gccttaacct gatgcgcgac ggggtaacgc ccagcgtggc gctggacatg 13380 accgcgcgca acatggaacc gggcatgtat gcctcaaacc ggccgtttat caaccgccta 13440 atggactact tgcatcgcgc ggccgccgtg aaccccgagt atttcaccaa tgccatcttg 13500 aacccgcact ggctaccgcc ccctggtttc tacaccgggg gattcgaggt gcccgagggt 13560 aacgatggat tcctctggga cgacatagac gacagcgtgt tttccccgca accgcagacc 13620 ctgctagagt tgcaacagcg cgagcaggca gaggcggcgc tgcgaaagga aagcttccgc 13680 aggccaagca gcttgtccga tctaggcgct gcggccccgc ggtcagatgc tagtagccca 13740 tttccaagct tgatagggtc tcttaccagc actcgcacca cccgcccgcg cctgctgggc 13800 gaggaggagt acctaaacaa ctcgctgctg cagccgcagc gcgaaaaaaa cctgcctccg 13860 gcatttccca acaacgggat agagagccta gtggacaaga tgagtagatg gaagacgtac 13920 gcgcaggagc acagggacgt gccaggcccg cgcccgccca cccgtcgtca aaggcacgac 13980 cgtcagcggg gtctggtgtg ggaggacgat gactcggcag acgacagcag cgtcctggat 14040 ttgggaggga gtggcaaccc gtttgcgcac cttcgcccca ggctggggag aatgttttaa 14100 aaaaaaaaaa aaagcatgat gcaaaataaa aaactcacca aggccattgg gccaggattc 14160 tcctcgacgt caccagcctg cttcagcagg ctgaagttag tagctccgct tcccttatag 14220 agctcgttca tgccctcggt gatgccggcg gcggtcagga actccagcag caccatgtgg 14280 tcccgcttct cgttggggtc cttgaacagg gcgctctggt agctcaggta gtggttgtcg 14340 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggccagctgc 14400 acgccgccgt cctcgatgtt gtgccggatc ttgaagttgg ccttgatgcc gttcttctgc 14460 ttgtcggcgg tgatgtacac gttgtggctg ttgtagttgt actccagctt gtggcccagg 14520 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tccggttcac cagggtgtcg 14580 ccctcgaact tcacctcggc ccgggtcttg tagttgccgt cgtccttgaa gaagatggtc 14640 cgctcctgca cgtagccctc gggcatggcg ctcttgaaga agtcgtgctg cttcatgtgg 14700 tcggggtacc gggcgaagca ctgcacgccg tagcccaggg tggtcaccag ggtgggccag 14760 ggcacgggca gcttgccggt ggtgcacagc agcttcaggg tcagcttgcc gtaggtggcg 14820 tcgccctcgc cctcgccgct cacgctgaac ttgtggccgt tcacgtcgcc gtccagctcc 14880 accaggatgg gcaccacgcc ggtgaacagc tcttcgcctt tgctcaccat ggcaccgagc 14940 gttggttttc ttgtattccc cttagtatgc ggcgcgcggc gatgtatgag gaaggtcctc 15000 ctccctccta cgagagtgtg gtgagcgcgg cgccagtggc ggcggcgctg ggttctccct 15060 tcgatgctcc cctggacccg ccgtttgtgc ctccgcggta cctgcggcct accgggggga 15120 gaaacagcat ccgttactct gagttggcac ccctattcga caccacccgt gtgtacctgg 15180 tggacaacaa gtcaacggat gtggcatccc tgaactacca gaacgaccac agcaactttc 15240 tgaccacggt cattcaaaac aatgactaca gcccggggga ggcaagcaca cagaccatca 15300 atcttgacga ccggtcgcac tggggcggcg acctgaaaac catcctgcat accaacatgc 15360 caaatgtgaa cgagttcatg tttaccaata agtttaaggc gcgggtgatg gtgtcgcgct 15420 tgcctactaa ggacaatcag gtggagctga aatacgagtg ggtggagttc acgctgcccg 15480 agggcaacta ctccgagacc atgaccatag accttatgaa caacgcgatc gtggagcact 15540 acttgaaagt gggcagacag aacggggttc tggaaagcga catcggggta aagtttgaca 15600 cccgcaactt cagactgggg tttgaccccg tcactggtct tgtcatgcct ggggtatata 15660 caaacgaagc cttccatcca gacatcattt tgctgccagg atgcggggtg gacttcaccc 15720 acagccgcct gagcaacttg ttgggcatcc gcaagcggca acccttccag gagggcttta 15780 ggatcaccta cgatgatctg gagggtggta acattcccgc actgttggat gtggacgcct 15840 accaggcgag cttgaaagat gacaccgaac agggcggggg tggcgcaggc ggcagcaaca 15900 gcagtggcag cggcgcggaa gagaactcca acgcggcagc cgcggcaatg cagccggtgg 15960 aggacatgaa cgatcatgcc attcgcggcg acacctttgc cacacgggct gaggagaagc 16020 gcgctgaggc cgaagcagcg gccgaagctg ccgcccccgc tgcgcaaccc gaggtcgaga 16080 agcctcagaa gaaaccggtg atcaaacccc tgacagagga cagcaagaaa cgcagttaca 16140 acctaataag caatgacagc accttcaccc agtaccgcag ctggtacctt gcatacaact 16200 acggcgaccc tcagaccgga atccgctcat ggaccctgct ttgcactcct gacgtaacct 16260 gcggctcgga gcaggtctac tggtcgttgc cagacatgat gcaagacccc gtgaccttcc 16320 gctccacgcg ccagatcagc aactttccgg tggtgggcgc cgagctgttg cccgtgcact 16380 ccaagagctt ctacaacgac caggccgtct actcccaact catccgccag tttacctctc 16440 tgacccacgt gttcaatcgc tttcccgaga accagatttt ggcgcgcccg ccagccccca 16500 ccatcaccac cgtcagtgaa aacgttcctg ctctcacaga tcacgggacg ctaccgctgc 16560 gcaacagcat cggaggagtc cagcgagtga ccattactga cgccagacgc cgcacctgcc 16620 cctacgttta caaggccctg ggcatagtct cgccgcgcgt cctatcgagc cgcacttttt 16680 gagcaagcat gtccatcctt atatcgccca gcaataacac aggctggggc ctgcgcttcc 16740 caagcaagat gtttggcggg gccaagaagc gctccgacca acacccagtg cgcgtgcgcg 16800 ggcactaccg cgcgccctgg ggcgcgcaca aacgcggccg cactgggcgc accaccgtcg 16860 atgacgccat cgacgcggtg gtggaggagg cgcgcaacta cacgcccacg ccgccaccag 16920 tgtccacagt ggacgcggcc attcagaccg tggtgcgcgg agcccggcgc tatgctaaaa 16980 tgaagagacg gcggaggcgc gtagcacgtc gccaccgccg ccgacccggc actgccgccc 17040 aacgcgcggc ggcggccctg cttaaccgcg cacgtcgcac cggccgacgg gcggccatgc 17100 gggccgctcg aaggctggcc gcgggtattg tcactgtgcc ccccaggtcc aggcgacgag 17160 cggccgccgc agcagccgcg gccattagtg ctatgactca gggtcgcagg ggcaacgtgt 17220 attgggtgcg cgactcggtt agcggcctgc gcgtgcccgt gcgcacccgc cccccgcgca 17280 actagattgc aagaaaaaac tacttagact cgtactgttg tatgtatcca gcggcggcgg 17340 cgcgcaacga agctatgtcc aagcgcaaaa tcaaagaaga gatgctccag gtcatcgcgc 17400 cggagatcta tggccccccg aagaaggaag agcaggatta caagccccga aagctaaagc 17460 gggtcaaaaa gaaaaagaaa gatgatgatg atgaacttga cgacgaggtg gaactgctgc 17520 acgctaccgc gcccaggcga cgggtacagt ggaaaggtcg acgcgtaaaa cgtgttttgc 17580 gacccggcac caccgtagtc tttacgcccg gtgagcgctc cacccgcacc tacaagcgcg 17640 tgtatgatga ggtgtacggc gacgaggacc tgcttgagca ggccaacgag cgcctcgggg 17700 agtttgccta cggaaagcgg cataaggaca tgctggcgtt gccgctggac gagggcaacc 17760 caacacctag cctaaagccc gtaacactgc agcaggtgct gcccgcgctt gcaccgtccg 17820 aagaaaagcg cggcctaaag cgcgagtctg gtgacttggc acccaccgtg cagctgatgg 17880 tacccaagcg ccagcgactg gaagatgtct tggaaaaaat gaccgtggaa cctgggctgg 17940 agcccgaggt ccgcgtgcgg ccaatcaagc aggtggcgcc gggactgggc gtgcagaccg 18000 tggacgttca gatacccact accagtagca ccagtattgc caccgccaca gagggcatgg 18060 agacacaaac gtccccggtt gcctcagcgg tggcggatgc cgcggtgcag gcggtcgctg 18120 cggccgcgtc caagacctct acggaggtgc aaacggaccc gtggatgttt cgcgtttcag 18180 ccccccggcg cccgcgccgt tcgaggaagt acggcgccgc cagcgcgcta ctgcccgaat 18240 atgccctaca tccttccatt gcgcctaccc ccggctatcg tggctacacc taccgcccca 18300 gaagacgagc aactacccga cgccgaacca ccactggaac ccgccgccgc cgtcgccgtc 18360 gccagcccgt gctggccccg atttccgtgc gcagggtggc tcgcgaagga ggcaggaccc 18420 tggtgctgcc aacagcgcgc taccacccca gcatcgttta aaagccggtc tttgtggttc 18480 ttgcagatat ggccctcacc tgccgcctcc gtttcccggt gccgggattc cgaggaagaa 18540 tgcaccgtag gaggggcatg gccggccacg gcctgacggg cggcatgcgt cgtgcgcacc 18600 accggcggcg gcgcgcgtcg caccgtcgca tgcgcggcgg tatcctgccc ctccttattc 18660 cactgatcgc cgcggcgatt ggcgccgtgc ccggaattgc atccgtggcc ttgcaggcgc 18720 agagacactg attaaaaaca agttgcatgt ggaaaaatca aaataaaaag tctggactct 18780 cacgctcgct tggtcctgta actattttgt agaatggaag acatcaactt tgcgtctctg 18840 gccccgcgac acggctcgcg cccgttcatg ggaaactggc aagatatcgg caccagcaat 18900 atgagcggtg gcgccttcag ctggggctcg ctgtggagcg gcattaaaaa tttcggttcc 18960 accgttaaga actatggcag caaggcctgg aacagcagca caggccagat gctgagggat 19020 aagttgaaag agcaaaattt ccaacaaaag gtggtagatg gcctggcctc tggcattagc 19080 ggggtggtgg acctggccaa ccaggcagtg caaaataaga ttaacagtaa gcttgatccc 19140 cgccctcccg tagaggagcc tccaccggcc gtggagacag tgtctccaga ggggcgtggc 19200 gaaaagcgtc cgcgccccga cagggaagaa actctggtga cgcaaataga cgagcctccc 19260 tcgtacgagg aggcactaaa gcaaggcctg cccaccaccc gtcccatcgc gcccatggct 19320 accggagtgc tgggccagca cacacccgta acgctggacc tgcctccccc cgccgacacc 19380 cagcagaaac ctgtgctgcc aggcccgacc gccgttgttg taacccgtcc tagccgcgcg 19440 tccctgcgcc gcgccgccag cggtccgcga tcgttgcggc ccgtagccag tggcaactgg 19500 caaagcacac tgaacagcat cgtgggtctg ggggtgcaat ccctgaagcg ccgacgatgc 19560 ttctgaatag ctaacgtgtc gtatgtgtgt catgtatgcg tccatgtcgc cgccagagga 19620 gctgctgagc cgccgcgcgc ccgctttcca agatggctac cccttcgatg atgccgcagt 19680 ggtcttacat gcacatctcg ggccaggacg cctcggagta cctgagcccc gggctggtgc 19740 agtttgcccg cgccaccgag acgtacttca gcctgaataa caagtttaga aaccccacgg 19800 tggcgcctac gcacgacgtg accacagacc ggtcccagcg tttgacgctg cggttcatcc 19860 ctgtggaccg tgaggatact gcgtactcgt acaaggcgcg gttcacccta gctgtgggtg 19920 ataaccgtgt gctggacatg gcttccacgt actttgacat ccgcggcgtg ctggacaggg 19980 gccctacttt taagccctac tctggcactg cctacaacgc cctggctccc aagggtgccc 20040 caaatccttg cgaatgggat gaagctgcta ctgctcttga aataaaccta gaagaagagg 20100 acgatgacaa cgaagacgaa gtagacgagc aagctgagca gcaaaaaact cacgtatttg 20160 ggcaggcgcc ttattctggt ataaatatta caaaggaggg tattcaaata ggtgtcgaag 20220 gtcaaacacc taaatatgcc gataaaacat ttcaacctga acctcaaata ggagaatctc 20280 agtggtacga aactgaaatt aatcatgcag ctgggagagt ccttaaaaag actaccccaa 20340 tgaaaccatg ttacggttca tatgcaaaac ccacaaatga aaatggaggg caaggcattc 20400 ttgtaaagca acaaaatgga aagctagaaa gtcaagtgga aatgcaattt ttctcaacta 20460 ctgaggcgac cgcaggcaat ggtgataact tgactcctaa agtggtattg tacagtgaag 20520 atgtagatat agaaacccca gacactcata tttcttacat gcccactatt aaggaaggta 20580 actcacgaga actaatgggc caacaatcta tgcccaacag gcctaattac attgctttta 20640 gggacaattt tattggtcta atgtattaca acagcacggg taatatgggt gttctggcgg 20700 gccaagcatc gcagttgaat gctgttgtag atttgcaaga cagaaacaca gagctttcat 20760 accagctttt gcttgattcc attggtgata gaaccaggta cttttctatg tggaatcagg 20820 ctgttgacag ctatgatcca gatgttagaa ttattgaaaa tcatggaact gaagatgaac 20880 ttccaaatta ctgctttcca ctgggaggtg tgattaatac agagactctt accaaggtaa 20940 aacctaaaac aggtcaggaa aatggatggg aaaaagatgc tacagaattt tcagataaaa 21000 atgaaataag agttggaaat aattttgcca tggaaatcaa tctaaatgcc aacctgtgga 21060 gaaatttcct gtactccaac atagcgctgt atttgcccga caagctaaag tacagtcctt 21120 ccaacgtaaa aatttctgat aacccaaaca cctacgacta catgaacaag cgagtggtgg 21180 ctcccgggtt agtggactgc tacattaacc ttggagcacg ctggtccctt gactatatgg 21240 acaacgtcaa cccatttaac caccaccgca atgctggcct gcgctaccgc tcaatgttgc 21300 tgggcaatgg tcgctatgtg cccttccaca tccaggtgcc tcagaagttc tttgccatta 21360 aaaacctcct tctcctgccg ggctcataca cctacgagtg gaacttcagg aaggatgtta 21420 acatggttct gcagagctcc ctaggaaatg acctaagggt tgacggagcc agcattaagt 21480 ttgatagcat ttgcctttac gccaccttct tccccatggc ccacaacacc gcctccacgc 21540 ttgaggccat gcttagaaac gacaccaacg accagtcctt taacgactat ctctccgccg 21600 ccaacatgct ctaccctata cccgccaacg ctaccaacgt gcccatatcc atcccctccc 21660 gcaactgggc ggctttccgc ggctgggcct tcacgcgcct taagactaag gaaaccccat 21720 cactgggctc gggctacgac ccttattaca cctactctgg ctctataccc tacctagatg 21780 gaacctttta cctcaaccac acctttaaga aggtggccat tacctttgac tcttctgtca 21840 gctggcctgg caatgaccgc ctgcttaccc ccaacgagtt tgaaattaag cgctcagttg 21900 acggggaggg ttacaacgtt gcccagtgta acatgaccaa agactggttc ctggtacaaa 21960 tgctagctaa ctacaacatt ggctaccagg gcttctatat cccagagagc tacaaggacc 22020 gcatgtactc cttctttaga aacttccagc ccatgagccg tcaggtggtg gatgatacta 22080 aatacaagga ctaccaacag gtgggcatcc tacaccaaca caacaactct ggatttgttg 22140 gctaccttgc ccccaccatg cgcgaaggac aggcctaccc tgctaacttc ccctatccgc 22200 ttataggcaa gaccgcagtt gacagcatta cccagaaaaa gtttctttgc gatcgcaccc 22260 tttggcgcat cccattctcc agtaacttta tgtccatggg cgcactcaca gacctgggcc 22320 aaaaccttct ctacgccaac tccgcccacg cgctagacat gacttttgag gtggatccca 22380 tggacgagcc cacccttctt tatgttttgt ttgaagtctt tgacgtggtc cgtgtgcacc 22440 ggccgcaccg cggcgtcatc gaaaccgtgt acctgcgcac gcccttctcg gccggcaacg 22500 ccacaacata aagaagcaag caacatcaac aacagctgcc gccatgggct ccagtgagca 22560 ggaactgaaa gccattgtca aagatcttgg ttgtgggcca tattttttgg gcacctatga 22620 caagcgcttt ccaggctttg tttctccaca caagctcgcc tgcgccatag tcaatacggc 22680 cggtcgcgag actgggggcg tacactggat ggcctttgcc tggaacccgc actcaaaaac 22740 atgctacctc tttgagccct ttggcttttc tgaccagcga ctcaagcagg tttaccagtt 22800 tgagtacgag tcactcctgc gccgtagcgc cattgcttct tcccccgacc gctgtataac 22860 gctggaaaag tccacccaaa gcgtacaggg gcccaactcg gccgcctgtg gactattctg 22920 ctgcatgttt ctccacgcct ttgccaactg gccccaaact cccatggatc acaaccccac 22980 catgaacctt attaccgggg tacccaactc catgctcaac agtccccagg tacagcccac 23040 cctgcgtcgc aaccaggaac agctctacag cttcctggag cgccactcgc cctacttccg 23100 cagccacagt gcgcagatta ggagcgccac ttctttttgt cacttgaaaa acatgtaaaa 23160 ataatgtact agagacactt tcaataaagg caaatgcttt tatttgtaca ctctcgggtg 23220 attatttacc cccacccttg ccgtctgcgc cgtttaaaaa tcaaaggggt tctgccgcgc 23280 atcgctatgc gccactggca gggacacgtt gcgatactgg tgtttagtgc tccacttaaa 23340 ctcaggcaca accatccgcg gcagctcggt gaagttttca ctccacaggc tgcgcaccat 23400 caccaacgcg tttagcaggt cgggcgccga tatcttgaag tcgcagttgg ggcctccgcc 23460 ctgcgcgcgc gagttgcgat acacagggtt gcagcactgg aacactatca gcgccgggtg 23520 gtgcacgctg gccagcacgc tcttgtcgga gatcagatcc gcgtccaggt cctccgcgtt 23580 gctcagggcg aacggagtca actttggtag ctgccttccc aaaaagggcg cgtgcccagg 23640 ctttgagttg cactcgcacc gtagtggcat caaaaggtga ccgtgcccgg tctgggcgtt 23700 aggatacagc gcctgcataa aagccttgat ctgcttaaaa gccacctgag cctttgcgcc 23760 ttcagagaag aacatgccgc aagacttgcc ggaaaactga ttggccggac aggccgcgtc 23820 gtgcacgcag caccttgcgt cggtgttgga gatctgcacc acatttcggc cccaccggtt 23880 cttcacgatc ttggccttgc tagactgctc cttcagcgcg cgctgcccgt tttcgctcgt 23940 cacatccatt tcaatcacgt gctccttatt tatcataatg cttccgtgta gacacttaag 24000 ctcgccttcg atctcagcgc agcggtgcag ccacaacgcg cagcccgtgg gctcgtgatg 24060 cttgtaggtc acctctgcaa acgactgcag gtacgcctgc aggaatcgcc ccatcatcgt 24120 cacaaaggtc ttgttgctgg tgaaggtcag ctgcaacccg cggtgctcct cgttcagcca 24180 ggtcttgcat acggccgcca gagcttccac ttggtcaggc agtagtttga agttcgcctt 24240 tagatcgtta tccacgtggt acttgtccat cagcgcgcgc gcagcctcca tgcccttctc 24300 ccacgcagac acgatcggca cactcagcgg gttcatcacc gtaatttcac tttccgcttc 24360 gctgggctct tcctcttcct cttgcgtccg cataccacgc gccactgggt cgtcttcatt 24420 cagccgccgc actgtgcgct tacctccttt gccatgcttg attagcaccg gtgggttgct 24480 gaaacccacc atttgtagcg ccacatcttc tctttcttcc tcgctgtcca cgattacctc 24540 tggtgatggc gggcgctcgg gcttgggaga agggcgcttc tttttcttct tgggcgcaat 24600 ggccaaatcc gccgccgagg tcgatggccg cgggctgggt gtgcgcggca ccagcgcgtc 24660 ttgtgatgag tcttcctcgt cctcggactc gatacgccgc ctcatccgct tttttggggg 24720 cgcccgggga ggcggcggcg acggggacgg ggacgacacg tcctccatgg ttgggggacg 24780 tcgcgccgca ccgcgtccgc gctcgggggt ggtttcgcgc tgctcctctt cccgactggc 24840 catttccttc tcctataggc agaaaaagat catggagtca gtcgagaaga aggacagcct 24900 aaccgccccc tctgagttcg ccaccaccgc ctccaccgat gccgccaacg cgcctaccac 24960 cttccccgtc gaggcacccc cgcttgagga ggaggaagtg attatcgagc aggacccagg 25020 ttttgtaagc gaagacgacg aggaccgctc agtaccaaca gaggataaaa agcaagacca 25080 ggacaacgca gaggcaaacg aggaacaagt cgggcggggg gacgaaaggc atggcgacta 25140 cctagatgtg ggagacgacg tgctgttgaa gcatctgcag cgccagtgcg ccattatctg 25200 cgacgcgttg caagagcgca gcgatgtgcc cctcgccata gcggatgtca gccttgccta 25260 cgaacgccac ctattctcac cgcgcgtacc ccccaaacgc caagaaaacg gcacatgcga 25320 gcccaacccg cgcctcaact tctaccccgt atttgccgtg ccagaggtgc ttgccaccta 25380 tcacatcttt ttccaaaact gcaagatacc cctatcctgc cgtgccaacc gcagccgagc 25440 ggacaagcag ctggccttgc ggcagggcgc tgtcatacct gatatcgcct cgctcaacga 25500 agtgccaaaa atctttgagg gtcttggacg cgacgagaag cgcgcggcaa acgctctgca 25560 acaggaaaac agcgaaaatg aaagtcactc tggagtgttg gtggaactcg agggtgacaa 25620 cgcgcgccta gccgtactaa aacgcagcat cgaggtcacc cactttgcct acccggcact 25680 taacctaccc cccaaggtca tgagcacagt catgagtgag ctgatcgtgc gccgtgcgca 25740 gcccctggag agggatgcaa atttgcaaga acaaacagag gagggcctac ccgcagttgg 25800 cgacgagcag ctagcgcgct ggcttcaaac gcgcgagcct gccgacttgg aggagcgacg 25860 caaactaatg atggccgcag tgctcgttac cgtggagctt gagtgcatgc agcggttctt 25920 tgctgacccg gagatgcagc gcaagctaga ggaaacattg cactacacct ttcgacaggg 25980 ctacgtacgc caggcctgca agatctccaa cgtggagctc tgcaacctgg tctcctacct 26040 tggaattttg cacgaaaacc gccttgggca aaacgtgctt cattccacgc tcaagggcga 26100 ggcgcgccgc gactacgtcc gcgactgcgt ttacttattt ctatgctaca cctggcagac 26160 ggccatgggc gtttggcagc agtgcttgga ggagtgcaac ctcaaggagc tgcagaaact 26220 gctaaagcaa aacttgaagg acctatggac ggccttcaac gagcgctccg tggccgcgca 26280 cctggcggac atcattttcc ccgaacgcct gcttaaaacc ctgcaacagg gtctgccaga 26340 cttcaccagt caaagcatgt tgcagaactt taggaacttt atcctagagc gctcaggaat 26400 cttgcccgcc acctgctgtg cacttcctag cgactttgtg cccattaagt accgcgaatg 26460 ccctccgccg ctttggggcc actgctacct tctgcagcta gccaactacc ttgcctacca 26520 ctctgacata atggaagacg tgagcggtga cggtctactg gagtgtcact gtcgctgcaa 26580 cctatgcacc ccgcaccgct ccctggtttg caattcgcag ctgcttaacg aaagtcaaat 26640 tatcggtacc tttgagctgc agggtccctc gcctgacgaa aagtccgcgg ctccggggtt 26700 gaaactcact ccggggctgt ggacgtcggc ttaccttcgc aaatttgtac ctgaggacta 26760 ccacgcccac gagattaggt tctacgaaga ccaatcccgc ccgccaaatg cggagcttac 26820 cgcctgcgtc attacccagg gccacattct tggccaattg caagccatca acaaagcccg 26880 ccaagagttt ctgctacgaa agggacgggg ggtttacttg gacccccagt ccggcgagga 26940 gctcaaccca atccccccgc cgccgcagcc ctatcagcag cagccgcggg cccttgcttc 27000 ccaggatggc acccaaaaag aagctgcagc tgccgccgcc acccacggac gaggaggaat 27060 actgggacag tcaggcagag gaggttttgg acgaggagga ggaggacatg atggaagact 27120 gggagagcct agacgaggaa gcttccgagg tcgaagaggt gtcagacgaa acaccgtcac 27180 cctcggtcgc attcccctcg ccggcgcccc agaaatcggc aaccggttcc agcatggcta 27240 caacctccgc tcctcaggcg ccgccggcac tgcccgttcg ccgacccaac cgtagatggg 27300 acaccactgg aaccagggcc ggtaagtcca agcagccgcc gccgttagcc caagagcaac 27360 aacagcgcca aggctaccgc tcatggcgcg ggcacaagaa cgccatagtt gcttgcttgc 27420 aagactgtgg gggcaacatc tccttcgccc gccgctttct tctctaccat cacggcgtgg 27480 ccttcccccg taacatcctg cattactacc gtcatctcta cagcccatac tgcaccggcg 27540 gcagcggcag cggcagcaac agcagcggcc acacagaagc aaaggcgacc ggatagcaag 27600 actctgacaa agcccaagaa atccacagcg gcggcagcag caggaggagg agcgctgcgt 27660 ctggcgccca acgaacccgt atcgacccgc gagcttagaa acaggatttt tcccactctg 27720 tatgctatat ttcaacagag caggggccaa gaacaagagc tgaaaataaa aaacaggtct 27780 ctgcgatccc tcacccgcag ctgcctgtat cacaaaagcg aagatcagct tcggcgcacg 27840 ctggaagacg cggaggctct cttcagtaaa tactgcgcgc tgactcttaa ggactagttt 27900 cgcgcccttt ctcaaattta agcgcgaaaa ctacgtcatc tccagcggcc acacccggcg 27960 ccagcacctg tcgtcagcgc catttcaact ttgtatacaa aagttgtgat gagcaaggaa 28020 attcccacgc cctacatgtg gagttaccag ccacaaatgg gacttgcggc tggagctgcc 28080 caagactact caacccgaat aaactacatg agcgcgggac cccacatgat atcccgggtc 28140 aacggaatac gcgcccaccg aaaccgaatt ctcctggaac aggcggctat taccaccaca 28200 cctcgtaata accttaatcc ccgtagttgg cccgctgccc tggtgtacca ggaaagtccc 28260 gctcccacca ctgtggtact tcccagagac gcccaggccg aagttcagat gactaactca 28320 ggggcgcagc ttgcgggcgg ctttcgtcac agggtgcggt cgcccgggca gggtataact 28380 cacctgacaa tcagagggcg aggtattcag ctcaacgacg agtcggtgag ctcctcgctt 28440 ggtctccgtc cggacgggac atttcagatc ggcggcgccg gccgctcttc attcacgcct 28500 cgtcaggcaa tcctaactct gcagacctcg tcctctgagc cgcgctctgg aggcattgga 28560 actctgcaat ttattgagga gtttgtgcca tcggtctact ttaacccctt ctcgggacct 28620 cccggccact atccggatca atttattcct aactttgacg cggtaaagga ctcggcggac 28680 ggctacgact gaatgttaag tggagaggca gagcaactgc gcctgaaaca cctggtccac 28740 tgtcgccgcc acaagtgctt tgcccgcgac tccggtgagt tttgctactt tgaattgccc 28800 gaggatcata tcgagggccc ggcgcacggc gtccggctta ccgcccaggg agagcttgcc 28860 cgtagcctga ttcgggagtt tacccagcgc cccctgctag ttgagcggga caggggaccc 28920 tgtgttctca ctgtgatttg caactgtcct aaccctggat tacatcaaga tctttgttgc 28980 catctctgtg ctgagtataa taaatacaga aattaaaata tactggggct cctatcgcca 29040 tcctgtaaac gccaccgtct tcacccgccc aagcaaacca aggcgaacct tacctggtac 29100 ttttaacatc tctccctctg tgatttacaa cagtttcaac ccagacggag tgagtctacg 29160 agagaacctc tccgagctca gctactccat cagaaaaaac accaccctcc ttacctgccg 29220 ggaacgtacg agtgcgtcac cggccgctgc accacaccta ccgcctgacc gtaaaccaga 29280 ctttttccgg acagacctca ataactctgt ttaccagaac aggaggtgag cttagaaaac 29340 ccttagggta ttaggccaaa ggcgcagcta ctgtggggtt tatgaacaat tcaagcaact 29400 ctacgggcta ttctaattca ggtttctcta gaatcggggt tggggttatt ctctgtcttg 29460 tgattctctt tattcttata ctaacgcttc tctgcctaag gctcgccgcc tgctgtgtgc 29520 acatttgcat ttattgtcag ctttttaaac gctggggtcg ccacccaaga tgattaggta 29580 cataatccta ggtttactca cccttgcgtc agcccacggt accacccaaa aggtggattt 29640 taaggagcca gcctgtaatg ttacattcgc agctgaagct aatgagtgca ccactcttat 29700 aaaatgcacc acagaacatg aaaagctgct tattcgccac aaaaacaaaa ttggcaagta 29760 tgctgtttat gctatttggc agccaggtga cactacagag tataatgtta cagttttcca 29820 gggtaaaagt cataaaactt ttatgtatac ttttccattt tatgaaatgt gcgacattac 29880 catgtacatg agcaaacagt ataagttgtg gcccccacaa aattgtgtgg aaaacactgg 29940 cactttctgc tgcactgcta tgctaattac agtgctcgct ttggtctgta ccctactcta 30000 tattaaatac aaaagcagac gcagctttat tgaggaaaag aaaatgcctt aatttactaa 30060 gttacaaagc taatgtcacc actaactgct ttactcgctg cttgcaaaac aaattcaaaa 30120 agttagcatt ataattagaa taggatttaa accccccggt catttcctgc tcaataccat 30180 tcccctgaac aattgactct atgtgggata tgctccagcg ctacaacctt gaagtcaggc 30240 ttcctggatg tcagcatctg actttggcca gcacctgtcc cgcggatttg ttccagtcca 30300 actacagcga cccaccctaa cagagatgac caacacaacc aacgcggccg ccgctaccgg 30360 acttacatct accacaaata caccccaagt ttctgccttt gtcaataact gggataactt 30420 gggcatgtgg tggttctcca tagcgcttat gtttgtatgc cttattatta tgtggctcat 30480 ctgctgccta aagcgcaaac gcgcccgacc acccatctat agtcccatca ttgtgctaca 30540 cccaaacaat gatggaatcc atagattgga cggactgaaa cacatgttct tttctcttac 30600 agtatgatta aatgagacat gattcctcga gtttttatat tactgaccct tgttgcgctt 30660 ttttgtgcgt gctccacatt ggctgcggtt tctcacatcg aagtagactg cattccagcc 30720 ttcacagtct atttgcttta cggatttgtc accctcacgc tcatctgcag cctcatcact 30780 gtggtcatcg cctttatcca gtgcattgac tgggtctgtg tgcgctttgc atatctcaga 30840 caccatcccc agtacaggga caggactata gctgagcttc ttagaattct ttaattatga 30900 aatttactgt gacttttctg ctgattattt gcaccctatc tgcgttttgt tccccgacct 30960 ccaagcctca aagacatata tcatgcagat tcactcgtat atggaatatt ccaagttgct 31020 acaatgaaaa aagcgatctt tccgaagcct ggttatatgc aatcatctct gttatggtgt 31080 tctgcagtac catcttagcc ctagctatat atccctacct tgacattggc tggaacgcaa 31140 tagatgccat gaaccaccca actttccccg cgcccgctat gcttccactg caacaagttg 31200 ttgccggcgg ctttgtccca gccaatcagc ctcgcccacc ttctcccacc cccactgaaa 31260 tcagctactt taatctaaca ggaggagatg actgacaccc tagatctaga aatggacgga 31320 attattacag agcagcgcct gctagaaaga cgcagggcag cggccgagca acagcgcatg 31380 aatcaagagc tccaagacat ggttaacttg caccagtgca aaaggggtat cttttgtctg 31440 gtaaagcagg ccaaagtcac ctacgacagt aataccaccg gacaccgcct tagctacaag 31500 ttgccaacca agcgtcagaa attggtggtc atggtgggag aaaagcccat taccataact 31560 cagcactcgg tagaaaccga aggctgcatt cactcacctt gtcaaggacc tgaggatctc 31620 tgcaccctta ttaagaccct gtgcggtctc aaagatctta ttccctttaa ctaataaaaa 31680 aaaataataa agcatcactt acttaaaatc agttagcaaa tttctgtcca gtttattcag 31740 cagcacctcc ttgccctcct cccagctctg gtattgcagc ttcctcctgg ctgcaaactt 31800 tctccacaat ctaaatggaa tgtcagtttc ctcctgttcc tgtccatccg cacccactat 31860 cttcatgttg ttgcagatga agcgcgcaag accgtctgaa gataccttca accccgtgta 31920 tccatatgac acggaaaccg gtcctccaac tgtgcctttt cttactcctc cctttgtatc 31980 ccccaatggg tttcaagaga gtccccctgg ggtactctct ttgcgcctat ccgaacctct 32040 agttacctcc aatggcatgc ttgcgctcaa aatgggcaac ggcctctctc tggacgaggc 32100 cggcaacctt acctcccaaa atgtaaccac tgtgagccca cctctcaaaa aaaccaagtc 32160 aaacataaac ctggaaatat ctgcacccct cacagttacc tcagaagccc taactgtggc 32220 tgccgccgca cctctaatgg tcgcgggcaa cacactcacc atgcaatcac aggccccgct 32280 aaccgtgcac gactccaaac ttagcattgc cacccaagga cccctcacag tgtcagaagg 32340 aaagctagcc ctgcaaacat caggccccct caccaccacc gatagcagta cccttactat 32400 cactgcctca ccccctctaa ctactgccac tggtagcttg ggcattgact tgaaagagcc 32460 catttataca caaaatggaa aactaggact aaagtacggg gctcctttgc atgtaacaga 32520 cgacctaaac actttgaccg tagcaactgg tccaggtgtg actattaata atacttcctt 32580 gcaaactaaa gttactggag ccttgggttt tgattcacaa ggcaatatgc aacttaatgt 32640 agcaggagga ctaaggattg attctcaaaa cagacgcctt atacttgatg ttagttatcc 32700 gtttgatgct caaaaccaac taaatctaag actaggacag ggccctcttt ttataaactc 32760 agcccacaac ttggatatta actacaacaa aggcctttac ttgtttacag cttcaaacaa 32820 ttccaaaaag cttgaggtta acctaagcac tgccaagggg ttgatgtttg acgctacagc 32880 catagccatt aatgcaggag atgggcttga atttggttca cctaatgcac caaacacaaa 32940 tcccctcaaa acaaaaattg gccatggcct agaatttgat tcaaacaagg ctatggttcc 33000 taaactagga actggcctta gttttgacag cacaggtgcc attacagtag gaaacaaaaa 33060 taatgataag ctaactttgt ggaccacacc agctccatct cctaactgta gactaaatgc 33120 agagaaagat gctaaactca ctttggtctt aacaaaatgt ggcagtcaaa tacttgctac 33180 agtttcagtt ttggctgtta aaggcagttt ggctccaata tctggaacag ttcaaagtgc 33240 tcatcttatt ataagatttg acgaaaatgg agtgctacta aacaattcct tcctggaccc 33300 agaatattgg aactttagaa atggagatct tactgaaggc acagcctata caaacgctgt 33360 tggatttatg cctaacctat cagcttatcc aaaatctcac ggtaaaactg ccaaaagtaa 33420 cattgtcagt caagtttact taaacggaga caaaactaaa cctgtaacac taaccattac 33480 actaaacggt acacaggaaa caggagacac aactccaagt gcatactcta tgtcattttc 33540 atgggactgg tctggccaca actacattaa tgaaatattt gccacatcct cttacacttt 33600 ttcatacatt gcccaagaat aaagaatcgt ttgtgttatg tttcaacgtg tttatttttc 33660 aattgcagaa aatttcgaat catttttcat tcagtagtat agccccacca ccacatagct 33720 tatacagatc accgtacctc aactttgtat aataaagttg taatcaaact cacagaaccc 33780 tagtattcaa cctgccacct ccctcccaac acacagagta cacagtcctt tctccccggc 33840 tggccttaaa aagcatcata tcatgggtaa cagacatatt cttaggtgtt atattccaca 33900 cggtttcctg tcgagccaaa cgctcatcag tgatattaat aaactccccg ggcagctcac 33960 ttaagttcat gtcgctgtcc agctgctgag ccacaggctg ctgtccaact tgcggttgct 34020 taacgggcgg cgaaggagaa gtccacgcct acatgggggt agagtcataa tcgtgcatca 34080 ggatagggcg gtggtgctgc agcagcgcgc gaataaactg ctgccgccgc cgctccgtcc 34140 tgcaggaata caacatggca gtggtctcct cagcgatgat tcgcaccgcc cgcagcataa 34200 ggcgccttgt cctccgggca cagcagcgca ccctgatctc acttaaatca gcacagtaac 34260 tgcagcacag caccacaata ttgttcaaaa tcccacagtg caaggcgctg tatccaaagc 34320 tcatggcggg gaccacagaa cccacgtggc catcatacca caagcgcagg tagattaagt 34380 ggcgacccct cataaacacg ctggacataa acattacctc ttttggcatg ttgtaattca 34440 ccacctcccg gtaccatata aacctctgat taaacatggc gccatccacc accatcctaa 34500 accagctggc caaaacctgc ccgccggcta tacactgcag ggaaccggga ctggaacaat 34560 gacagtggag agcccaggac tcgtaaccat ggatcatcat gctcgtcatg atatcaatgt 34620 tggcacaaca caggcacacg tgcatacact tcctcaggat tacaagctcc tcccgcgtta 34680 gaaccatatc ccagggaaca acccattcct gaatcagcgt aaatcccaca ctgcagggaa 34740 gacctcgcac gtaactcacg ttgtgcattg tcaaagtgtt acattcgggc agcagcggat 34800 gatcctccag tatggtagcg cgggtttctg tctcaaaagg aggtagacga tccctactgt 34860 acggagtgcg ccgagacaac cgagatcgtg ttggtcgtag tgtcatgcca aatggaacgc 34920 cggacgtagt catatttcct gaagcaaaac caggtgcggg cgtgacaaac agatctgcgt 34980 ctccggtctc gccgcttaga tcgctctgtg tagtagttgt agtatatcca ctctctcaaa 35040 gcatccaggc gccccctggc ttcgggttct atgtaaactc cttcatgcgc cgctgccctg 35100 ataacatcca ccaccgcaga ataagccaca cccagccaac ctacacattc gttctgcgag 35160 tcacacacgg gaggagcggg aagagctgga agaaccatgt tttttttttt attccaaaag 35220 attatccaaa acctcaaaat gaagatctat taagtgaacg cgctcccctc cggtggcgtg 35280 gtcaaactct acagccaaag aacagataat ggcatttgta agatgttgca caatggcttc 35340 caaaaggcaa acggccctca cgtccaagtg gacgtaaagg ctaaaccctt cagggtgaat 35400 ctcctctata aacattccag caccttcaac catgcccaaa taattctcat ctcgccacct 35460 tctcaatata tctctaagca aatcccgaat attaagtccg gccattgtaa aaatctgctc 35520 cagagcgccc tccaccttca gcctcaagca gcgaatcatg attgcaaaaa ttcaggttcc 35580 tcacagacct gtataagatt caaaagcgga acattaacaa aaataccgcg atcccgtagg 35640 tcccttcgca gggccagctg aacataatcg tgcaggtctg cacggaccag cgcggccact 35700 tccccgccag gaaccatgac aaaagaaccc acactgatta tgacacgcat actcggagct 35760 atgctaacca gcgtagcccc gatgtaagct tgttgcatgg gcggcgatat aaaatgcaag 35820 gtgctgctca aaaaatcagg caaagcctcg cgcaaaaaag aaagcacatc gtagtcatgc 35880 tcatgcagat aaaggcaggt aagctccgga accaccacag aaaaagacac catttttctc 35940 tcaaacatgt ctgcgggttt ctgcataaac acaaaataaa ataacaaaaa aacatttaaa 36000 cattagaagc ctgtcttaca acaggaaaaa caacccttat aagcataaga cggactacgg 36060 ccatgccggc gtgaccgtaa aaaaactggt caccgtgatt aaaaagcacc accgacagct 36120 cctcggtcat gtccggagtc ataatgtaag actcggtaaa cacatcaggt tgattcacat 36180 cggtcagtgc taaaaagcga ccgaaatagc ccgggggaat acatacccgc aggcgtagag 36240 acaacattac agcccccata ggaggtataa caaaattaat aggagagaaa aacacataaa 36300 cacctgaaaa accctcctgc ctaggcaaaa tagcaccctc ccgctccaga acaacataca 36360 gcgcttccac agcggcagcc ataacagtca gccttaccag taaaaaagaa aacctattaa 36420 aaaaacacca ctcgacacgg caccagctca atcagtcaca gtgtaaaaaa gggccaagtg 36480 cagagcgagt atatatagga ctaaaaaatg acgtaacggt taaagtccac aaaaaacacc 36540 cagaaaaccg cacgcgaacc tacgcccaga aacgaaagcc aaaaaaccca caacttcctc 36600 aaatcgtcac ttccgttttc ccacgttacg tcacttccca ttttaagaaa actacaattc 36660 ccaacacata caagttactc cgccctaaaa cctacgtcac ccgccccgtt cccacgcccc 36720 gcgccacgtc acaaactcca ccccctcatt atcatattgg cttcaatcca aaataaggta 36780 tattattgat gatg 36794 <210> 6 <211> 36785 <212> DNA <213> Artificial Sequence <220> <223> Synthetic adenovirus genome DBP-P2A-YPet <400> 6 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420 cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480 tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540 tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600 aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660 tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720 cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780 gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840 cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900 ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960 cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020 caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080 ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140 tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200 gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260 ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320 cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380 ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440 gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500 cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560 ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620 gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680 cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740 ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800 tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860 gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920 caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980 gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040 agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100 aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160 cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220 gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280 gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340 gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400 gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460 tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520 ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580 tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640 agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700 tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760 gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820 acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880 gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940 aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000 ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060 gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120 tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180 acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240 aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300 tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360 gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420 tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480 ttggctctag cgatgaagat acagattgag gtactgaaat gtgtgggcgt ggcttaaggg 3540 tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg 3600 ccgccgccca acttttctat acaaagttgt aatgagcacc aactcgtttg atggaagcat 3660 tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat 3720 gggctccagc attgatggtc gccccgtcct gcccgcaaac tctactacct tgacctacga 3780 gaccgtgtct ggaacgccgt tggagactgc agcctccgcc gccgcttcag ccgctgcagc 3840 caccgcccgc gggattgtga ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc 3900 ttcccgttca tccgcccgcg atgacaagtt gacggctctt ttggcacaat tggattcttt 3960 gacccgggaa cttaatgtcg tttctcagca gctgttggat ctgcgccagc aggtttctgc 4020 cctgaaggct tcctcccctc ccaatgcggt ttaaaacata aataaaaaac cagactctgt 4080 ttggatttgg atcaagcata agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg 4140 taggcccggg accagcggtc tcggtcgttg agggtcctgt gtattttttc caggacgtgg 4200 taaaggtgac tctggatgtt cagatacatg ggcataagcc cgtctctggg gtggaggtag 4260 caccactgca gagcttcatg ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag 4320 cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc tgattgccag gggcaggccc 4380 ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt gcatacgtgg ggatatgaga 4440 tgcatcttgg actgtatttt taggttggct atgttcccag ccatatccct ccggggattc 4500 atgttgtgca gaaccaccag cacagtgtat ccggtgcact tgggaaattt gtcatgtagc 4560 ttagaaggaa atgcgtggaa gaacttggag acgcccttgt gacctccaag attttccatg 4620 cattcgtcca taatgatggc aatgggccca cgggcggcgg cctgggcgaa gatatttctg 4680 ggatcactaa cgtcatagtt gtgttccagg atgagatcgt cataggccat ttttacaaag 4740 cgcgggcgga gggtgccaga ctgcggtata atggttccat ccggcccagg ggcgtagtta 4800 ccctcacaga tttgcatttc ccacgctttg agttcagatg gggggatcat gtctacctgc 4860 ggggcgatga agaaaacggt ttccggggta ggggagatca gctgggaaga aagcaggttc 4920 ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa tcacacctat taccgggtgc 4980 aactggtagt taagagagct gcagctgccg tcatccctga gcaggggggc cacttcgtta 5040 agcatgtccc tgactcgcat gttttccctg accaaatccg ccagaaggcg ctcgccgccc 5100 agcgatagca gttcttgcaa ggaagcaaag tttttcaacg gtttgagacc gtccgccgta 5160 ggcatgcttt tgagcgtttg accaagcagt tccaggcggt cccacagctc ggtcacctgc 5220 tctacggcat ctcgatccag catatctcct cgtttcgcgg gttggggcgg ctttcgctgt 5280 acggcagtag tcggtgctcg tccagacggg ccagggtcat gtctttccac gggcgcaggg 5340 tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc tccgggctgc gcgctggcca 5400 gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt 5460 cggccaggta gcatttgacc atggtgtcat agtccagccc ctccgcggcg tggcccttgg 5520 cgcgcagctt gcccttggag gaggcgccgc acgaggggca gtgcagactt ttgagggcgt 5580 agagcttggg cgcgagaaat accgattccg gggagtaggc atccgcgccg caggccccgc 5640 agacggtctc gcattccacg agccaggtga gctctggccg ttcggggtca aaaaccaggt 5700 ttcccccatg ctttttgatg cgtttcttac ctctggtttc catgagccgg tgtccacgct 5760 cggtgacgaa aaggctgtcc gtgtccccgt atacagactt gagaggcctg tcctcgagcg 5820 gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacaaag gctcgcgtcc 5880 aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact agggggtcca 5940 ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag gtgattggtt 6000 tgtaggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag ggggtggggg 6060 cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt tggggtgagt 6120 actccctctg aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg 6180 aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgca tccatctggt 6240 cagaaaagac aatctttttg ttgtcaagct tggtggcaaa cgacccgtag agggcgttgg 6300 acagcaactt ggcgatggag cgcagggttt ggtttttgtc gcgatcggcg cgctccttgg 6360 ccgcgatgtt tagctgcacg tattcgcgcg caacgcaccg ccattcggga aagacggtgg 6420 tgcgctcgtc gggcaccagg tgcacgcgcc aaccgcggtt gtgcagggtg acaaggtcaa 6480 cgctggtggc tacctctccg cgtaggcgct cgttggtcca gcagaggcgg ccgcccttgc 6540 gcgagcagaa tggcggtagg gggtctagct gcgtctcgtc cggggggtct gcgtccacgg 6600 taaagacccc gggcagcagg cgcgcgtcga agtagtctat cttgcatcct tgcaagtcta 6660 gcgcctgctg ccatgcgcgg gcggcaagcg cgcgctcgta tgggttgagt gggggacccc 6720 atggcatggg gtgggtgagc gcggaggcgt acatgccgca aatgtcgtaa acgtagaggg 6780 gctctctgag tattccaaga tatgtagggt agcatcttcc accgcggatg ctggcgcgca 6840 cgtaatcgta tagttcgtgc gagggagcga ggaggtcggg accgaggttg ctacgggcgg 6900 gctgctctgc tcggaagact atctgcctga agatggcatg tgagttggat gatatggttg 6960 gacgctggaa gacgttgaag ctggcgtctg tgagacctac cgcgtcacgc acgaaggagg 7020 cgtaggagtc gcgcagcttg ttgaccagct cggcggtgac ctgcacgtct agggcgcagt 7080 agtccagggt ttccttgatg atgtcatact tatcctgtcc cttttttttc cacagctcgc 7140 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct 7200 ccgaacggta agagcctagc atgtagaact ggttgacggc ctggtaggcg cagcatccct 7260 tttctacggg tagcgcgtat gcctgcgcgg ccttccggag cgaggtgtgg gtgagcgcaa 7320 aggtgtccct gaccatgact ttgaggtact ggtatttgaa gtcagtgtcg tcgcatccgc 7380 cctgctccca gagcaaaaag tccgtgcgct ttttggaacg cggatttggc agggcgaagg 7440 tgacatcgtt gaagagtatc tttcccgcgc gaggcataaa gttgcgtgtg atgcggaagg 7500 gtcccggcac ctcggaacgg ttgttaatta cctgggcggc gagcacgatc tcgtcaaagc 7560 cgttgatgtt gtggcccaca atgtaaagtt ccaagaagcg cgggatgccc ttgatggaag 7620 gcaatttttt aagttcctcg taggtgagct cttcagggga gctgagcccg tgctctgaaa 7680 gggcccagtc tgcaagatga gggttggaag cgacgaatga gctccacagg tcacgggcca 7740 ttagcatttg caggtggtcg cgaaaggtcc taaactggcg acctatggcc attttttctg 7800 gggtgatgca gtagaaggta agcgggtctt gttcccagcg gtcccatcca aggttcgcgg 7860 ctaggtctcg cgcggcagtc actagaggct catctccgcc gaacttcatg accagcatga 7920 agggcacgag ctgcttccca aaggccccca tccaagtata ggtctctaca tcgtaggtga 7980 caaagagacg ctcggtgcga ggatgcgagc cgatcgggaa gaactggatc tcccgccacc 8040 aattggagga gtggctattg atgtggtgaa agtagaagtc cctgcgacgg gccgaacact 8100 cgtgctggct tttgtaaaaa cgtgcgcagt actggcagcg gtgcacgggc tgtacatcct 8160 gcacgaggtt gacctgacga ccgcgcacaa ggaagcagag tgggaatttg agcccctcgc 8220 ctggcgggtt tggctggtgg tcttctactt cggctgcttg tccttgaccg tctggctgct 8280 cgaggggagt tacggtggat cggaccacca cgccgcgcga gcccaaagtc cagatgtccg 8340 cgcgcggcgg tcggagcttg atgacaacat cgcgcagatg ggagctgtcc atggtctgga 8400 gctcccgcgg cgtcaggtca ggcgggagct cctgcaggtt tacctcgcat agacgggtca 8460 gggcgcgggc tagatccagg tgatacctaa tttccagggg ctggttggtg gcggcgtcga 8520 tggcttgcaa gaggccgcat ccccgcggcg cgactacggt accgcgcggc gggcggtggg 8580 ccgcgggggt gtccttggat gatgcatcta aaagcggtga cgcgggcgag cccccggagg 8640 tagggggggc tccggacccg ccgggagagg gggcaggggc acgtcggcgc cgcgcgcggg 8700 caggagctgg tgctgcgcgc gtaggttgct ggcgaacgcg acgacgcggc ggttgatctc 8760 ctgaatctgg cgcctctgcg tgaagacgac gggcccggtg agcttgagcc tgaaagagag 8820 ttcgacagaa tcaatttcgg tgtcgttgac ggcggcctgg cgcaaaatct cctgcacgtc 8880 tcctgagttg tcttgatagg cgatctcggc catgaactgc tcgatctctt cctcctggag 8940 atctccgcgt ccggctcgct ccacggtggc ggcgaggtcg ttggaaatgc gggccatgag 9000 ctgcgagaag gcgttgaggc ctccctcgtt ccagacgcgg ctgtagacca cgcccccttc 9060 ggcatcgcgg gcgcgcatga ccacctgcgc gagattgagc tccacgtgcc gggcgaagac 9120 ggcgtagttt cgcaggcgct gaaagaggta gttgagggtg gtggcggtgt gttctgccac 9180 gaagaagtac ataacccagc gtcgcaacgt ggattcgttg atatccccca aggcctcaag 9240 gcgctccatg gcctcgtaga agtccacggc gaagttgaaa aactgggagt tgcgcgccga 9300 cacggttaac tcctcctcca gaagacggat gagctcggcg acagtgtcgc gcacctcgcg 9360 ctcaaaggct acaggggcct cttcttcttc ttcaatctcc tcttccataa gggcctcccc 9420 ttcttcttct tctggcggcg gtgggggagg ggggacacgg cggcgacgac ggcgcaccgg 9480 gaggcggtcg acaaagcgct cgatcatctc cccgcggcga cggcgcatgg tctcggtgac 9540 ggcgcggccg ttctcgcggg ggcgcagttg gaagacgccg cccgtcatgt cccggttatg 9600 ggttggcggg gggctgccat gcggcaggga tacggcgcta acgatgcatc tcaacaattg 9660 ttgtgtaggt actccgccgc cgagggacct gagcgagtcc gcatcgaccg gatcggaaaa 9720 cctctcgaga aaggcgtcta accagtcaca gtcgcaaggt aggctgagca ccgtggcggg 9780 cggcagcggg cggcggtcgg ggttgtttct ggcggaggtg ctgctgatga tgtaattaaa 9840 gtaggcggtc ttgagacggc ggatggtcga cagaagcacc atgtccttgg gtccggcctg 9900 ctgaatgcgc aggcggtcgg ccatgcccca ggcttcgttt tgacatcggc gcaggtcttt 9960 gtagtagtct tgcatgagcc tttctaccgg cacttcttct tctccttcct cttgtcctgc 10020 atctcttgca tctatcgctg cggcggcggc ggagtttggc cgtaggtggc gccctcttcc 10080 tcccatgcgt gtgaccccga agcccctcat cggctgaagc agggctaggt cggcgacaac 10140 gcgctcggct aatatggcct gctgcacctg cgtgagggta gactggaagt catccatgtc 10200 cacaaagcgg tggtatgcgc ccgtgttgat ggtgtaagtg cagttggcca taacggacca 10260 gttaacggtc tggtgacccg gctgcgagag ctcggtgtac ctgagacgcg agtaagccct 10320 cgagtcaaat acgtagtcgt tgcaagtccg caccaggtac tggtatccca ccaaaaagtg 10380 cggcggcggc tggcggtaga ggggccagcg tagggtggcc ggggctccgg gggcgagatc 10440 ttccaacata aggcgatgat atccgtagat gtacctggac atccaggtga tgccggcggc 10500 ggtggtggag gcgcgcggaa agtcgcggac gcggttccag atgttgcgca gcggcaaaaa 10560 gtgctccatg gtcgggacgc tctggccggt caggcgcgcg caatcgttga cgctctagac 10620 cgtgcaaaag gagagcctgt aagcgggcac tcttccgtgg tctggtggat aaattcgcaa 10680 gggtatcatg gcggacgacc ggggttcgag ccccgtatcc ggccgtccgc cgtgatccat 10740 gcggttaccg cccgcgtgtc gaacccaggt gtgcgacgtc agacaacggg ggagtgctcc 10800 ttttggcttc cttccaggcg cggcggctgc tgcgctagct tttttggcca ctggccgcgc 10860 gcagcgtaag cggttaggct ggaaagcgaa agcattaagt ggctcgctcc ctgtagccgg 10920 agggttattt tccaagggtt gagtcgcggg acccccggtt cgagtctcgg accggccgga 10980 ctgcggcgaa cgggggtttg cctccccgtc atgcaagacc ccgcttgcaa attcctccgg 11040 aaacagggac gagccccttt tttgcttttc ccagatgcat ccggtgctgc ggcagatgcg 11100 cccccctcct cagcagcggc aagagcaaga gcagcggcag acatgcaggg caccctcccc 11160 tcctcctacc gcgtcaggag gggcgacatc cgcggttgac gcggcagcag atggtgatta 11220 cgaacccccg cggcgccggg cccggcacta cctggacttg gaggagggcg agggcctggc 11280 gcggctagga gcgccctctc ctgagcggta cccaagggtg cagctgaagc gtgatacgcg 11340 tgaggcgtac gtgccgcggc agaacctgtt tcgcgaccgc gagggagagg agcccgagga 11400 gatgcgggat cgaaagttcc acgcagggcg cgagctgcgg catggcctga atcgcgagcg 11460 gttgctgcgc gaggaggact ttgagcccga cgcgcgaacc gggattagtc ccgcgcgcgc 11520 acacgtggcg gccgccgacc tggtaaccgc atacgagcag acggtgaacc aggagattaa 11580 ctttcaaaaa agctttaaca accacgtgcg tacgcttgtg gcgcgcgagg aggtggctat 11640 aggactgatg catctgtggg actttgtaag cgcgctggag caaaacccaa atagcaagcc 11700 gctcatggcg cagctgttcc ttatagtgca gcacagcagg gacaacgagg cattcaggga 11760 tgcgctgcta aacatagtag agcccgaggg ccgctggctg ctcgatttga taaacatcct 11820 gcagagcata gtggtgcagg agcgcagctt gagcctggct gacaaggtgg ccgccatcaa 11880 ctattccatg cttagcctgg gcaagtttta cgcccgcaag atataccata ccccttacgt 11940 tcccatagac aaggaggtaa agatcgaggg gttctacatg cgcatggcgc tgaaggtgct 12000 taccttgagc gacgacctgg gcgtttatcg caacgagcgc atccacaagg ccgtgagcgt 12060 gagccggcgg cgcgagctca gcgaccgcga gctgatgcac agcctgcaaa gggccctggc 12120 tggcacgggc agcggcgata gagaggccga gtcctacttt gacgcgggcg ctgacctgcg 12180 ctgggcccca agccgacgcg ccctggaggc agctggggcc ggacctgggc tggcggtggc 12240 acccgcgcgc gctggcaacg tcggcggcgt ggaggaatat gacgaggacg atgagtacga 12300 gccagaggac ggcgagtact aagcggtgat gtttctgatc agatgatgca agacgcaacg 12360 gacccggcgg tgcgggcggc gctgcagagc cagccgtccg gccttaactc cacggacgac 12420 tggcgccagg tcatggaccg catcatgtcg ctgactgcgc gcaatcctga cgcgttccgg 12480 cagcagccgc aggccaaccg gctctccgca attctggaag cggtggtccc ggcgcgcgca 12540 aaccccacgc acgagaaggt gctggcgatc gtaaacgcgc tggccgaaaa cagggccatc 12600 cggcccgacg aggccggcct ggtctacgac gcgctgcttc agcgcgtggc tcgttacaac 12660 agcggcaacg tgcagaccaa cctggaccgg ctggtggggg atgtgcgcga ggccgtggcg 12720 cagcgtgagc gcgcgcagca gcagggcaac ctgggctcca tggttgcact aaacgccttc 12780 ctgagtacac agcccgccaa cgtgccgcgg ggacaggagg actacaccaa ctttgtgagc 12840 gcactgcggc taatggtgac tgagacaccg caaagtgagg tgtaccagtc tgggccagac 12900 tattttttcc agaccagtag acaaggcctg cagaccgtaa acctgagcca ggctttcaaa 12960 aacttgcagg ggctgtgggg ggtgcgggct cccacaggcg accgcgcgac cgtgtctagc 13020 ttgctgacgc ccaactcgcg cctgttgctg ctgctaatag cgcccttcac ggacagtggc 13080 agcgtgtccc gggacacata cctaggtcac ttgctgacac tgtaccgcga ggccataggt 13140 caggcgcatg tggacgagca tactttccag gagattacaa gtgtcagccg cgcgctgggg 13200 caggaggaca cgggcagcct ggaggcaacc ctaaactacc tgctgaccaa ccggcggcag 13260 aagatcccct cgttgcacag tttaaacagc gaggaggagc gcattttgcg ctacgtgcag 13320 cagagcgtga gccttaacct gatgcgcgac ggggtaacgc ccagcgtggc gctggacatg 13380 accgcgcgca acatggaacc gggcatgtat gcctcaaacc ggccgtttat caaccgccta 13440 atggactact tgcatcgcgc ggccgccgtg aaccccgagt atttcaccaa tgccatcttg 13500 aacccgcact ggctaccgcc ccctggtttc tacaccgggg gattcgaggt gcccgagggt 13560 aacgatggat tcctctggga cgacatagac gacagcgtgt tttccccgca accgcagacc 13620 ctgctagagt tgcaacagcg cgagcaggca gaggcggcgc tgcgaaagga aagcttccgc 13680 aggccaagca gcttgtccga tctaggcgct gcggccccgc ggtcagatgc tagtagccca 13740 tttccaagct tgatagggtc tcttaccagc actcgcacca cccgcccgcg cctgctgggc 13800 gaggaggagt acctaaacaa ctcgctgctg cagccgcagc gcgaaaaaaa cctgcctccg 13860 gcatttccca acaacgggat agagagccta gtggacaaga tgagtagatg gaagacgtac 13920 gcgcaggagc acagggacgt gccaggcccg cgcccgccca cccgtcgtca aaggcacgac 13980 cgtcagcggg gtctggtgtg ggaggacgat gactcggcag acgacagcag cgtcctggat 14040 ttgggaggga gtggcaaccc gtttgcgcac cttcgcccca ggctggggag aatgttttaa 14100 aaaaaaaaaa gcatgatgca aaataaaaaa ctcaccaagg ccatggcacc gagcgttggt 14160 tttcttgtat tccccttagt atgcggcgcg cggcgatgta tgaggaaggt cctcctccct 14220 cctacgagag tgtggtgagc gcggcgccag tggcggcggc gctgggttct cccttcgatg 14280 ctcccctgga cccgccgttt gtgcctccgc ggtacctgcg gcctaccggg gggagaaaca 14340 gcatccgtta ctctgagttg gcacccctat tcgacaccac ccgtgtgtac ctggtggaca 14400 acaagtcaac ggatgtggca tccctgaact accagaacga ccacagcaac tttctgacca 14460 cggtcattca aaacaatgac tacagcccgg gggaggcaag cacacagacc atcaatcttg 14520 acgaccggtc gcactggggc ggcgacctga aaaccatcct gcataccaac atgccaaatg 14580 tgaacgagtt catgtttacc aataagttta aggcgcgggt gatggtgtcg cgcttgccta 14640 ctaaggacaa tcaggtggag ctgaaatacg agtgggtgga gttcacgctg cccgagggca 14700 actactccga gaccatgacc atagacctta tgaacaacgc gatcgtggag cactacttga 14760 aagtgggcag acagaacggg gttctggaaa gcgacatcgg ggtaaagttt gacacccgca 14820 acttcagact ggggtttgac cccgtcactg gtcttgtcat gcctggggta tatacaaacg 14880 aagccttcca tccagacatc attttgctgc caggatgcgg ggtggacttc acccacagcc 14940 gcctgagcaa cttgttgggc atccgcaagc ggcaaccctt ccaggagggc tttaggatca 15000 cctacgatga tctggagggt ggtaacattc ccgcactgtt ggatgtggac gcctaccagg 15060 cgagcttgaa agatgacacc gaacagggcg ggggtggcgc aggcggcagc aacagcagtg 15120 gcagcggcgc ggaagagaac tccaacgcgg cagccgcggc aatgcagccg gtggaggaca 15180 tgaacgatca tgccattcgc ggcgacacct ttgccacacg ggctgaggag aagcgcgctg 15240 aggccgaagc agcggccgaa gctgccgccc ccgctgcgca acccgaggtc gagaagcctc 15300 agaagaaacc ggtgatcaaa cccctgacag aggacagcaa gaaacgcagt tacaacctaa 15360 taagcaatga cagcaccttc acccagtacc gcagctggta ccttgcatac aactacggcg 15420 accctcagac cggaatccgc tcatggaccc tgctttgcac tcctgacgta acctgcggct 15480 cggagcaggt ctactggtcg ttgccagaca tgatgcaaga ccccgtgacc ttccgctcca 15540 cgcgccagat cagcaacttt ccggtggtgg gcgccgagct gttgcccgtg cactccaaga 15600 gcttctacaa cgaccaggcc gtctactccc aactcatccg ccagtttacc tctctgaccc 15660 acgtgttcaa tcgctttccc gagaaccaga ttttggcgcg cccgccagcc cccaccatca 15720 ccaccgtcag tgaaaacgtt cctgctctca cagatcacgg gacgctaccg ctgcgcaaca 15780 gcatcggagg agtccagcga gtgaccatta ctgacgccag acgccgcacc tgcccctacg 15840 tttacaaggc cctgggcata gtctcgccgc gcgtcctatc gagccgcact ttttgagcaa 15900 gcatgtccat ccttatatcg cccagcaata acacaggctg gggcctgcgc ttcccaagca 15960 agatgtttgg cggggccaag aagcgctccg accaacaccc agtgcgcgtg cgcgggcact 16020 accgcgcgcc ctggggcgcg cacaaacgcg gccgcactgg gcgcaccacc gtcgatgacg 16080 ccatcgacgc ggtggtggag gaggcgcgca actacacgcc cacgccgcca ccagtgtcca 16140 cagtggacgc ggccattcag accgtggtgc gcggagcccg gcgctatgct aaaatgaaga 16200 gacggcggag gcgcgtagca cgtcgccacc gccgccgacc cggcactgcc gcccaacgcg 16260 cggcggcggc cctgcttaac cgcgcacgtc gcaccggccg acgggcggcc atgcgggccg 16320 ctcgaaggct ggccgcgggt attgtcactg tgccccccag gtccaggcga cgagcggccg 16380 ccgcagcagc cgcggccatt agtgctatga ctcagggtcg caggggcaac gtgtattggg 16440 tgcgcgactc ggttagcggc ctgcgcgtgc ccgtgcgcac ccgccccccg cgcaactaga 16500 ttgcaagaaa aaactactta gactcgtact gttgtatgta tccagcggcg gcggcgcgca 16560 acgaagctat gtccaagcgc aaaatcaaag aagagatgct ccaggtcatc gcgccggaga 16620 tctatggccc cccgaagaag gaagagcagg attacaagcc ccgaaagcta aagcgggtca 16680 aaaagaaaaa gaaagatgat gatgatgaac ttgacgacga ggtggaactg ctgcacgcta 16740 ccgcgcccag gcgacgggta cagtggaaag gtcgacgcgt aaaacgtgtt ttgcgacccg 16800 gcaccaccgt agtctttacg cccggtgagc gctccacccg cacctacaag cgcgtgtatg 16860 atgaggtgta cggcgacgag gacctgcttg agcaggccaa cgagcgcctc ggggagtttg 16920 cctacggaaa gcggcataag gacatgctgg cgttgccgct ggacgagggc aacccaacac 16980 ctagcctaaa gcccgtaaca ctgcagcagg tgctgcccgc gcttgcaccg tccgaagaaa 17040 agcgcggcct aaagcgcgag tctggtgact tggcacccac cgtgcagctg atggtaccca 17100 agcgccagcg actggaagat gtcttggaaa aaatgaccgt ggaacctggg ctggagcccg 17160 aggtccgcgt gcggccaatc aagcaggtgg cgccgggact gggcgtgcag accgtggacg 17220 ttcagatacc cactaccagt agcaccagta ttgccaccgc cacagagggc atggagacac 17280 aaacgtcccc ggttgcctca gcggtggcgg atgccgcggt gcaggcggtc gctgcggccg 17340 cgtccaagac ctctacggag gtgcaaacgg acccgtggat gtttcgcgtt tcagcccccc 17400 ggcgcccgcg cggttcgagg aagtacggcg ccgccagcgc gctactgccc gaatatgccc 17460 tacatccttc cattgcgcct acccccggct atcgtggcta cacctaccgc cccagaagac 17520 gagcaactac ccgacgccga accaccactg gaacccgccg ccgccgtcgc cgtcgccagc 17580 ccgtgctggc cccgatttcc gtgcgcaggg tggctcgcga aggaggcagg accctggtgc 17640 tgccaacagc gcgctaccac cccagcatcg tttaaaagcc ggtctttgtg gttcttgcag 17700 atatggccct cacctgccgc ctccgtttcc cggtgccggg attccgagga agaatgcacc 17760 gtaggagggg catggccggc cacggcctga cgggcggcat gcgtcgtgcg caccaccggc 17820 ggcggcgcgc gtcgcaccgt cgcatgcgcg gcggtatcct gcccctcctt attccactga 17880 tcgccgcggc gattggcgcc gtgcccggaa ttgcatccgt ggccttgcag gcgcagagac 17940 actgattaaa aacaagttgc atgtggaaaa atcaaaataa aaagtctgga ctctcacgct 18000 cgcttggtcc tgtaactatt ttgtagaatg gaagacatca actttgcgtc tctggccccg 18060 cgacacggct cgcgcccgtt catgggaaac tggcaagata tcggcaccag caatatgagc 18120 ggtggcgcct tcagctgggg ctcgctgtgg agcggcatta aaaatttcgg ttccaccgtt 18180 aagaactatg gcagcaaggc ctggaacagc agcacaggcc agatgctgag ggataagttg 18240 aaagagcaaa atttccaaca aaaggtggta gatggcctgg cctctggcat tagcggggtg 18300 gtggacctgg ccaaccaggc agtgcaaaat aagattaaca gtaagcttga tccccgccct 18360 cccgtagagg agcctccacc ggccgtggag acagtgtctc cagaggggcg tggcgaaaag 18420 cgtccgcgcc ccgacaggga agaaactctg gtgacgcaaa tagacgagcc tccctcgtac 18480 gaggaggcac taaagcaagg cctgcccacc acccgtccca tcgcgcccat ggctaccgga 18540 gtgctgggcc agcacacacc cgtaacgctg gacctgcctc cccccgccga cacccagcag 18600 aaacctgtgc tgccaggccc gaccgccgtt gttgtaaccc gtcctagccg cgcgtccctg 18660 cgccgcgccg ccagcggtcc gcgatcgttg cggcccgtag ccagtggcaa ctggcaaagc 18720 acactgaaca gcatcgtggg tctgggggtg caatccctga agcgccgacg atgcttctga 18780 atagctaacg tgtcgtatgt gtgtcatgta tgcgtccatg tcgccgccag aggagctgct 18840 gagccgccgc gcgcccgctt tccaagatgg ctaccccttc gatgatgccg cagtggtctt 18900 acatgcacat ctcgggccag gacgcctcgg agtacctgag ccccgggctg gtgcagtttg 18960 cccgcgccac cgagacgtac ttcagcctga ataacaagtt tagaaacccc acggtggcgc 19020 ctacgcacga cgtgaccaca gaccggtccc agcgtttgac gctgcggttc atccctgtgg 19080 accgtgagga tactgcgtac tcgtacaagg cgcggttcac cctagctgtg ggtgataacc 19140 gtgtgctgga catggcttcc acgtactttg acatccgcgg cgtgctggac aggggcccta 19200 cttttaagcc ctactctggc actgcctaca acgccctggc tcccaagggt gccccaaatc 19260 cttgcgaatg ggatgaagct gctactgctc ttgaaataaa cctagaagaa gaggacgatg 19320 acaacgaaga cgaagtagac gagcaagctg agcagcaaaa aactcacgta tttgggcagg 19380 cgccttattc tggtataaat attacaaagg agggtattca aataggtgtc gaaggtcaaa 19440 cacctaaata tgccgataaa acatttcaac ctgaacctca aataggagaa tctcagtggt 19500 acgaaactga aattaatcat gcagctggga gagtccttaa aaagactacc ccaatgaaac 19560 catgttacgg ttcatatgca aaacccacaa atgaaaatgg agggcaaggc attcttgtaa 19620 agcaacaaaa tggaaagcta gaaagtcaag tggaaatgca atttttctca actactgagg 19680 cgaccgcagg caatggtgat aacttgactc ctaaagtggt attgtacagt gaagatgtag 19740 atatagaaac cccagacact catatttctt acatgcccac tattaaggaa ggtaactcac 19800 gagaactaat gggccaacaa tctatgccca acaggcctaa ttacattgct tttagggaca 19860 attttattgg tctaatgtat tacaacagca cgggtaatat gggtgttctg gcgggccaag 19920 catcgcagtt gaatgctgtt gtagatttgc aagacagaaa cacagagctt tcataccagc 19980 ttttgcttga ttccattggt gatagaacca ggtacttttc tatgtggaat caggctgttg 20040 acagctatga tccagatgtt agaattattg aaaatcatgg aactgaagat gaacttccaa 20100 attactgctt tccactggga ggtgtgatta atacagagac tcttaccaag gtaaaaccta 20160 aaacaggtca ggaaaatgga tgggaaaaag atgctacaga attttcagat aaaaatgaaa 20220 taagagttgg aaataatttt gccatggaaa tcaatctaaa tgccaacctg tggagaaatt 20280 tcctgtactc caacatagcg ctgtatttgc ccgacaagct aaagtacagt ccttccaacg 20340 taaaaatttc tgataaccca aacacctacg actacatgaa caagcgagtg gtggctcccg 20400 ggttagtgga ctgctacatt aaccttggag cacgctggtc ccttgactat atggacaacg 20460 tcaacccatt taaccaccac cgcaatgctg gcctgcgcta ccgctcaatg ttgctgggca 20520 atggtcgcta tgtgcccttc cacatccagg tgcctcagaa gttctttgcc attaaaaacc 20580 tccttctcct gccgggctca tacacctacg agtggaactt caggaaggat gttaacatgg 20640 ttctgcagag ctccctagga aatgacctaa gggttgacgg agccagcatt aagtttgata 20700 gcatttgcct ttacgccacc ttcttcccca tggcccacaa caccgcctcc acgcttgagg 20760 ccatgcttag aaacgacacc aacgaccagt cctttaacga ctatctctcc gccgccaaca 20820 tgctctaccc tatacccgcc aacgctacca acgtgcccat atccatcccc tcccgcaact 20880 gggcggcttt ccgcggctgg gccttcacgc gccttaagac taaggaaacc ccatcactgg 20940 gctcgggcta cgacccttat tacacctact ctggctctat accctaccta gatggaacct 21000 tttacctcaa ccacaccttt aagaaggtgg ccattacctt tgactcttct gtcagctggc 21060 ctggcaatga ccgcctgctt acccccaacg agtttgaaat taagcgctca gttgacgggg 21120 agggttacaa cgttgcccag tgtaacatga ccaaagactg gttcctggta caaatgctag 21180 ctaactataa cattggctac cagggcttct atatcccaga gagctacaag gaccgcatgt 21240 actccttctt tagaaacttc cagcccatga gccgtcaggt ggtggatgat actaaataca 21300 aggactacca acaggtgggc atcctacacc aacacaacaa ctctggattt gttggctacc 21360 ttgcccccac catgcgcgaa ggacaggcct accctgctaa cttcccctat ccgcttatag 21420 gcaagaccgc agttgacagc attacccaga aaaagtttct ttgcgatcgc accctttggc 21480 gcatcccatt ctccagtaac tttatgtcca tgggcgcact cacagacctg ggccaaaacc 21540 ttctctacgc caactccgcc cacgcgctag acatgacttt tgaggtggat cccatggacg 21600 agcccaccct tctttatgtt ttgtttgaag tctttgacgt ggtccgtgtg caccagccgc 21660 accgcggcgt catcgaaacc gtgtacctgc gcacgccctt ctcggccggc aacgccacaa 21720 cataaagaag caagcaacat caacaacagc tgccgccatg ggctccagtg agcaggaact 21780 gaaagccatt gtcaaagatc ttggttgtgg gccatatttt ttgggcacct atgacaagcg 21840 ctttccaggc tttgtttctc cacacaagct cgcctgcgcc atagtcaata cggccggtcg 21900 cgagactggg ggcgtacact ggatggcctt tgcctggaac ccgcactcaa aaacatgcta 21960 cctctttgag ccctttggct tttctgacca gcgactcaag caggtttacc agtttgagta 22020 cgagtcactc ctgcgccgta gcgccattgc ttcttccccc gaccgctgta taacgctgga 22080 aaagtccacc caaagcgtac aggggcccaa ctcggccgcc tgtggactat tctgctgcat 22140 gtttctccac gcctttgcca actggcccca aactcccatg gatcacaacc ccaccatgaa 22200 ccttattacc ggggtaccca actccatgct caacagtccc caggtacagc ccaccctgcg 22260 tcgcaaccag gaacagctct acagcttcct ggagcgccac tcgccctact tccgcagcca 22320 cagtgcgcag attaggagcg ccacttcttt ttgtcacttg aaaaacatgt aaaaataatg 22380 tactagagac actttcaata aaggcaaatg cttttatttg tacactctcg ggtgattatt 22440 tacccccacc cttgccgtct gcgccgttta cttatagagc tcgttcatgc cctcggtgat 22500 gccggcggcg gtcaggaact ccagcagcac catgtggtcc cgcttctcgt tggggtcctt 22560 gaacagggcg ctctggtagc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc 22620 gatgggggtg ttctgctggt agtggtcggc cagctgcacg ccgccgtcct cgatgttgtg 22680 ccggatcttg aagttggcct tgatgccgtt cttctgcttg tcggcggtga tgtacacgtt 22740 gtggctgttg tagttgtact ccagcttgtg gcccaggatg ttgccgtcct ccttgaagtc 22800 gatgcccttc agctcgatcc ggttcaccag ggtgtcgccc tcgaacttca cctcggcccg 22860 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtccgc tcctgcacgt agccctcggg 22920 catggcgctc ttgaagaagt cgtgctgctt catgtggtcg gggtaccggg cgaagcactg 22980 cacgccgtag cccagggtgg tcaccagggt gggccagggc acgggcagct tgccggtggt 23040 gcacagcagc ttcagggtca gcttgccgta ggtggcgtcg ccctcgccct cgccgctcac 23100 gctgaacttg tggccgttca cgtcgccgtc cagctccacc aggatgggca ccacgccggt 23160 gaacagctct tcgcctttgc tcaccattgg gccaggattc tcctcgacgt caccagcctg 23220 cttcagcagg ctgaagttag tagctccgct tccaaaatca aaggggttct gccgcgcatc 23280 gctatgcgcc actggcaggg acacgttgcg atactggtgt ttagtgctcc acttaaactc 23340 aggcacaacc atccgcggca gctcggtgaa gttttcactc cacaggctgc gcaccatcac 23400 caacgcgttt agcaggtcgg gcgccgatat cttgaagtcg cagttggggc ctccgccctg 23460 cgcgcgcgag ttgcgataca cagggttgca gcactggaac actatcagcg ccgggtggtg 23520 cacgctggcc agcacgctct tgtcggagat cagatccgcg tccaggtcct ccgcgttgct 23580 cagggcgaac ggagtcaact ttggtagctg ccttcccaaa aagggcgcgt gcccaggctt 23640 tgagttgcac tcgcaccgta gtggcatcaa aaggtgaccg tgcccggtct gggcgttagg 23700 atacagcgcc tgcataaaag ccttgatctg cttaaaagcc acctgagcct ttgcgccttc 23760 agagaagaac atgccgcaag acttgccgga aaactgattg gccggacagg ccgcgtcgtg 23820 cacgcagcac cttgcgtcgg tgttggagat ctgcaccaca tttcggcccc accggttctt 23880 cacgatcttg gccttgctag actgctcctt cagcgcgcgc tgcccgtttt cgctcgtcac 23940 atccatttca atcacgtgct ccttatttat cataatgctt ccgtgtagac acttaagctc 24000 gccttcgatc tcagcgcagc ggtgcagcca caacgcgcag cccgtgggct cgtgatgctt 24060 gtaggtcacc tctgcaaacg actgcaggta cgcctgcagg aatcgcccca tcatcgtcac 24120 aaaggtcttg ttgctggtga aggtcagctg caacccgcgg tgctcctcgt tcagccaggt 24180 cttgcatacg gccgccagag cttccacttg gtcaggcagt agtttgaagt tcgcctttag 24240 atcgttatcc acgtggtact tgtccatcag cgcgcgcgca gcctccatgc ccttctccca 24300 cgcagacacg atcggcacac tcagcgggtt catcaccgta atttcacttt ccgcttcgct 24360 gggctcttcc tcttcctctt gcgtccgcat accacgcgcc actgggtcgt cttcattcag 24420 ccgccgcact gtgcgcttac ctcctttgcc atgcttgatt agcaccggtg ggttgctgaa 24480 acccaccatt tgtagcgcca catcttctct ttcttcctcg ctgtccacga ttacctctgg 24540 tgatggcggg cgctcgggct tgggagaagg gcgcttcttt ttcttcttgg gcgcaatggc 24600 caaatccgcc gccgaggtcg atggccgcgg gctgggtgtg cgcggcacca gcgcgtcttg 24660 tgatgagtct tcctcgtcct cggactcgat acgccgcctc atccgctttt ttgggggcgc 24720 ccggggaggc ggcggcgacg gggacgggga cgacacgtcc tccatggttg ggggacgtcg 24780 cgccgcaccg cgtccgcgct cgggggtggt ttcgcgctgc tcctcttccc gactggccat 24840 ttccttctcc tataggcaga aaaagatcat ggagtcagtc gagaagaagg acagcctaac 24900 cgccccctct gagttcgcca ccaccgcctc caccgatgcc gccaacgcgc ctaccacctt 24960 ccccgtcgag gcacccccgc ttgaggagga ggaagtgatt atcgagcagg acccaggttt 25020 tgtaagcgaa gacgacgagg accgctcagt accaacagag gataaaaagc aagaccagga 25080 caacgcagag gcaaacgagg aacaagtcgg gcggggggac gaaaggcatg gcgactacct 25140 agatgtggga gacgacgtgc tgttgaagca tctgcagcgc cagtgcgcca ttatctgcga 25200 cgcgttgcaa gagcgcagcg atgtgcccct cgccatagcg gatgtcagcc ttgcctacga 25260 acgccaccta ttctcaccgc gcgtaccccc caaacgccaa gaaaacggca catgcgagcc 25320 caacccgcgc ctcaacttct accccgtatt tgccgtgcca gaggtgcttg ccacctatca 25380 catctttttc caaaactgca agatacccct atcctgccgt gccaaccgca gccgagcgga 25440 caagcagctg gccttgcggc agggcgctgt catacctgat atcgcctcgc tcaacgaagt 25500 gccaaaaatc tttgagggtc ttggacgcga cgagaagcgc gcggcaaacg ctctgcaaca 25560 ggaaaacagc gaaaatgaaa gtcactctgg agtgttggtg gaactcgagg gtgacaacgc 25620 gcgcctagcc gtactaaaac gcagcatcga ggtcacccac tttgcctacc cggcacttaa 25680 cctacccccc aaggtcatga gcacagtcat gagtgagctg atcgtgcgcc gtgcgcagcc 25740 cctggagagg gatgcaaatt tgcaagaaca aacagaggag ggcctacccg cagttggcga 25800 cgagcagcta gcgcgctggc ttcaaacgcg cgagcctgcc gacttggagg agcgacgcaa 25860 actaatgatg gccgcagtgc tcgttaccgt ggagcttgag tgcatgcagc ggttctttgc 25920 tgacccggag atgcagcgca agctagagga aacattgcac tacacctttc gacagggcta 25980 cgtacgccag gcctgcaaga tctccaacgt ggagctctgc aacctggtct cctaccttgg 26040 aattttgcac gaaaaccgcc ttgggcaaaa cgtgcttcat tccacgctca agggcgaggc 26100 gcgccgcgac tacgtccgcg actgcgttta cttatttcta tgctacacct ggcagacggc 26160 catgggcgtt tggcagcagt gcttggagga gtgcaacctc aaggagctgc agaaactgct 26220 aaagcaaaac ttgaaggacc tatggacggc cttcaacgag cgctccgtgg ccgcgcacct 26280 ggcggacatc attttccccg aacgcctgct taaaaccctg caacagggtc tgccagactt 26340 caccagtcaa agcatgttgc agaactttag gaactttatc ctagagcgct caggaatctt 26400 gcccgccacc tgctgtgcac ttcctagcga ctttgtgccc attaagtacc gcgaatgccc 26460 tccgccgctt tggggccact gctaccttct gcagctagcc aactaccttg cctaccactc 26520 tgacataatg gaagacgtga gcggtgacgg tctactggag tgtcactgtc gctgcaacct 26580 atgcaccccg caccgctccc tggtttgcaa ttcgcagctg cttaacgaaa gtcaaattat 26640 cggtaccttt gagctgcagg gtccctcgcc tgacgaaaag tccgcggctc cggggttgaa 26700 actcactccg gggctgtgga cgtcggctta ccttcgcaaa tttgtacctg aggactacca 26760 cgcccacgag attaggttct acgaagacca atcccgcccg cctaatgcgg agcttaccgc 26820 ctgcgtcatt acccagggcc acattcttgg ccaattgcaa gccatcaaca aagcccgcca 26880 agagtttctg ctacgaaagg gacggggggt ttacttggac ccccagtccg gcgaggagct 26940 caacccaatc cccccgccgc cgcagcccta tcagcagcag ccgcgggccc ttgcttccca 27000 ggatggcacc caaaaagaag ctgcagctgc cgccgccacc cacggacgag gaggaatact 27060 gggacagtca ggcagaggag gttttggacg aggaggagga ggacatgatg gaagactggg 27120 agagcctaga cgaggaagct tccgaggtcg aagaggtgtc agacgaaaca ccgtcaccct 27180 cggtcgcatt cccctcgccg gcgccccaga aatcggcaac cggttccagc atggctacaa 27240 cctccgctcc tcaggcgccg ccggcactgc ccgttcgccg acccaaccgt agatgggaca 27300 ccactggaac cagggccggt aagtccaagc agccgccgcc gttagcccaa gagcaacaac 27360 agcgccaagg ctaccgctca tggcgcgggc acaagaacgc catagttgct tgcttgcaag 27420 actgtggggg caacatctcc ttcgcccgcc gctttcttct ctaccatcac ggcgtggcct 27480 tcccccgtaa catcctgcat tactaccgtc atctctacag cccatactgc accggcggca 27540 gcggcagcaa cagcagcggc cacacagaag caaaggcgac cggatagcaa gactctgaca 27600 aagcccaaga aatccacagc ggcggcagca gcaggaggag gagcgctgcg tctggcgccc 27660 aacgaacccg tatcgacccg cgagcttaga aacaggattt ttcccactct gtatgctata 27720 tttcaacaga gcaggggcca agaacaagag ctgaaaataa aaaacaggtc tctgcgatcc 27780 ctcacccgca gctgcctgta tcacaaaagc gaagatcagc ttcggcgcac gctggaagac 27840 gcggaggctc tcttcagtaa atactgcgcg ctgactctta aggactagtt tcgcgccctt 27900 tctcaaattt aagcgcgaaa actacgtcat ctccagcggc cacacccggc gccagcacct 27960 gtcgtcagcg ccatttcaac tttgtataca aaagttgtga tgagcaagga aattcccacg 28020 ccctacatgt ggagttacca gccacaaatg ggacttgcgg ctggagctgc ccaagactac 28080 tcaacccgaa taaactacat gagcgcggga ccccacatga tatcccgggt caacggaata 28140 cgcgcccacc gaaaccgaat tctcctggaa caggcggcta ttaccaccac acctcgtaat 28200 aaccttaatc cccgtagttg gcccgctgcc ctggtgtacc aggaaagtcc cgctcccacc 28260 actgtggtac ttcccagaga cgcccaggcc gaagttcaga tgactaactc aggggcgcag 28320 cttgcgggcg gctttcgtca cagggtgcgg tcgcccgggc agggtataac tcacctgaca 28380 atcagagggc gaggtattca gctcaacgac gagtcggtga gctcctcgct tggtctccgt 28440 ccggacggga catttcagat cggcggcgcc ggccgctctt cattcacgcc tcgtcaggca 28500 atcctaactc tgcagacctc gtcctctgag ccgcgctctg gaggcattgg aactctgcaa 28560 tttattgagg agtttgtgcc atcggtctac tttaacccct tctcgggacc tcccggccac 28620 tatccggatc aatttattcc taactttgac gcggtaaagg actcggcgga cggctacgac 28680 tgaatgttaa gtggagaggc agagcaactg cgcctgaaac acctggtcca ctgtcgccgc 28740 cacaagtgct ttgcccgcga ctccggtgag ttttgctact ttgaattgcc cgaggatcat 28800 atcgagggcc cggcgcacgg cgtccggctt accgcccagg gagagcttgc ccgtagcctg 28860 attcgggagt ttacccagcg ccccctgcta gttgagcggg acaggggacc ctgtgttctc 28920 actgtgattt gcaactgtcc taaccctgga ttacatcaag atctttgttg ccatctctgt 28980 gctgagtata ataaatacag aaattaaaat atactggggc tcctatcgcc atcctgtaaa 29040 cgccaccgtc ttcacccgcc caagcaaacc aaggcgaacc ttacctggta cttttaacat 29100 ctctccctct gtgatttaca acagtttcaa cccagacgga gtgagtctac gagagaacct 29160 ctccgagctc agctactcca tcagaaaaaa caccaccctc cttacctgcc gggaacgtac 29220 gagtgcgtca ccggccgctg caccacacct accgcctgac cgtaaaccag actttttccg 29280 gacagacctc aataactctg tttaccagaa caggaggtga gcttagaaaa cccttagggt 29340 attaggccaa aggcgcagct actgtggggt ttatgaacaa ttcaagcaac tctacgggct 29400 attctaattc aggtttctct agaatcgggg ttggggttat tctctgtctt gtgattctct 29460 ttattcttat actaacgctt ctctgcctaa ggctcgccgc ctgctgtgtg cacatttgca 29520 tttattgtca gctttttaaa cgctggggtc gccacccaag atgattaggt acataatcct 29580 aggtttactc acccttgcgt cagcccacgg taccacccaa aaggtggatt ttaaggagcc 29640 agcctgtaat gttacattcg cagctgaagc taatgagtgc accactctta taaaatgcac 29700 cacagaacat gaaaagctgc ttattcgcca caaaaacaaa attggcaagt atgctgttta 29760 tgctatttgg cagccaggtg acactacaga gtataatgtt acagttttcc agggtaaaag 29820 tcataaaact tttatgtata cttttccatt ttatgaaatg tgcgacatta ccatgtacat 29880 gagcaaacag tataagttgt ggcccccaca aaattgtgtg gaaaacactg gcactttctg 29940 ctgcactgct atgctaatta cagtgctcgc tttggtctgt accctactct atattaaata 30000 caaaagcaga cgcagcttta ttgaggaaaa gaaaatgcct taatttacta agttacaaag 30060 ctaatgtcac cactaactgc tttactcgct gcttgcaaaa caaattcaaa aagttagcat 30120 tataattaga ataggattta aaccccccgg tcatttcctg ctcaatacca ttcccctgaa 30180 caattgactc tatgtgggat atgctccagc gctacaacct tgaagtcagg cttcctggat 30240 gtcagcatct gactttggcc agcacctgtc ccgcggattt gttccagtcc aactacagcg 30300 acccacccta acagagatga ccaacacaac caacgcggcc gccgctaccg gacttacatc 30360 taccacaaat acaccccaag tttctgcctt tgtcaataac tgggataact tgggcatgtg 30420 gtggttctcc atagcgctta tgtttgtatg ccttattatt atgtggctca tctgctgcct 30480 aaagcgcaaa cgcgcccgac cacccatcta tagtcccatc attgtgctac acccaaacaa 30540 tgatggaatc catagattgg acggactgaa acacatgttc ttttctctta cagtatgatt 30600 aaatgagaca tgattcctcg agtttttata ttactgaccc ttgttgcgct tttttgtgcg 30660 tgctccacat tggctgcggt ttctcacatc gaagtagact gcattccagc cttcacagtc 30720 tatttgcttt acggatttgt caccctcacg ctcatctgca gcctcatcac tgtggtcatc 30780 gcctttatcc agtgcattga ctgggtctgt gtgcgctttg catatctcag acaccatccc 30840 cagtacaggg acaggactat agctgagctt cttagaattc tttaattatg aaatttactg 30900 tgacttttct gctgattatt tgcaccctat ctgcgttttg ttccccgacc tccaagcctc 30960 aaagacatat atcatgcaga ttcactcgta tatggaatat tccaagttgc tacaatgaaa 31020 aaagcgatct ttccgaagcc tggttatatg caatcatctc tgttatggtg ttctgcagta 31080 ccatcttagc cctagctata tatccctacc ttgacattgg ctggaacgca atagatgcca 31140 tgaaccaccc aactttcccc gcgcccgcta tgcttccact gcaacaagtt gttgccggcg 31200 gctttgtccc agccaatcag cctcgcccac cttctcccac ccccactgaa atcagctact 31260 ttaatctaac aggaggagat gactgacacc ctagatctag aaatggacgg aattattaca 31320 gagcagcgcc tgctagaaag acgcagggca gcggccgagc aacagcgcat gaatcaagag 31380 ctccaagaca tggttaactt gcaccagtgc aaaaggggta tcttttgtct ggtaaagcag 31440 gccaaagtca cctacgacag taataccacc ggacaccgcc ttagctacaa gttgccaacc 31500 aagcgtcaga aattggtggt catggtggga gaaaagccca ttaccataac tcagcactcg 31560 gtagaaaccg aaggctgcat tcactcacct tgtcaaggac ctgaggatct ctgcaccctt 31620 attaagaccc tgtgcggtct caaagatctt attcccttta actaataaaa aaaaataata 31680 aagcatcact tacttaaaat cagttagcaa atttctgtcc agtttattca gcagcacctc 31740 cttgccctcc tcccagctct ggtattgcag cttcctcctg gctgcaaact ttctccacaa 31800 tctaaatgga atgtcagttt cctcctgttc ctgtccatcc gcacccacta tcttcatgtt 31860 gttgcagatg aagcgcgcaa gaccgtctga agataccttc aaccccgtgt atccatatga 31920 cacggaaacc ggtcctccaa ctgtgccttt tcttactcct ccctttgtat cccccaatgg 31980 gtttcaagag agtccccctg gggtactctc tttgcgccta tccgaacctc tagttacctc 32040 caatggcatg cttgcgctca aaatgggcaa cggcctctct ctggacgagg ccggcaacct 32100 tacctcccaa aatgtaacca ctgtgagccc acctctcaaa aaaaccaagt caaacataaa 32160 cctggaaata tctgcacccc tcacagttac ctcagaagcc ctaactgtgg ctgccgccgc 32220 acctctaatg gtcgcgggca acacactcac catgcaatca caggccccgc taaccgtgca 32280 cgactccaaa cttagcattg ccacccaagg acccctcaca gtgtcagaag gaaagctagc 32340 cctgcaaaca tcaggccccc tcaccaccac cgatagcagt acccttacta tcactgcctc 32400 accccctcta actactgcca ctggtagctt gggcattgac ttgaaagagc ccatttatac 32460 acaaaatgga aaactaggac taaagtacgg ggctcctttg catgtaacag acgacctaaa 32520 cactttgacc gtagcaactg gtccaggtgt gactattaat aatacttcct tgcaaactaa 32580 agttactgga gccttgggtt ttgattcaca aggcaatatg caacttaatg tagcaggagg 32640 actaaggatt gattctcaaa acagacgcct tatacttgat gttagttatc cgtttgatgc 32700 tcaaaaccaa ctaaatctaa gactaggaca gggccctctt tttataaact cagcccacaa 32760 cttggatatt aactacaaca aaggccttta cttgtttaca gcttcaaaca attccaaaaa 32820 gcttgaggtt aacctaagca ctgccaaggg gttgatgttt gacgctacag ccatagccat 32880 taatgcagga gatgggcttg aatttggttc acctaatgca ccaaacacaa atcccctcaa 32940 aacaaaaatt ggccatggcc tagaatttga ttcaaacaag gctatggttc ctaaactagg 33000 aactggcctt agttttgaca gcacaggtgc cattacagta ggaaacaaaa ataatgataa 33060 gctaactttg tggaccacac cagctccatc tcctaactgt agactaaatg cagagaaaga 33120 tgctaaactc actttggtct taacaaaatg tggcagtcaa atacttgcta cagtttcagt 33180 tttggctgtt aaaggcagtt tggctccaat atctggaaca gttcaaagtg ctcatcttat 33240 tataagattt gacgaaaatg gagtgctact aaacaattcc ttcctggacc cagaatattg 33300 gaactttaga aatggagatc ttactgaagg cacagcctat acaaacgctg ttggatttat 33360 gcctaaccta tcagcttatc caaaatctca cggtaaaact gccaaaagta acattgtcag 33420 tcaagtttac ttaaacggag acaaaactaa acctgtaaca ctaaccatta cactaaacgg 33480 tacacaggaa acaggagaca caactccaag tgcatactct atgtcatttt catgggactg 33540 gtctggccac aactacatta atgaaatatt tgccacatcc tcttacactt tttcatacat 33600 tgcccaagaa taaagaatcg tttgtgttat gtttcaacgt gtttattttt caattgcaga 33660 aaatttcgaa tcatttttca ttcagtagta tagccccacc accacatagc ttatacagat 33720 caccgtacct caactttgta taataaagtt gtaatcaaac tcacagaacc ctagtattca 33780 acctgccacc tccctcccaa cacacagagt acacagtcct ttctccccgg ctggccttaa 33840 aaagcatcat atcatgggta acagacatat tcttaggtgt tatattccac acggtttcct 33900 gtcgagccaa acgctcatca gtgatattaa taaactcccc gggcagctca cttaagttca 33960 tgtcgctgtc cagctgctga gccacaggct gctgtccaac ttgcggttgc ttaacgggcg 34020 gcgaaggaga agtccacgcc tacatggggg tagagtcata atcgtgcatc aggatagggc 34080 ggtggtgctg cagcagcgcg cgaataaact gctgccgccg ccgctccgtc ctgcaggaat 34140 acaacatggc agtggtctcc tcagcgatga ttcgcaccgc ccgcagcata aggcgccttg 34200 tcctccgggc acagcagcgc accctgatct cacttaaatc agcacagtaa ctgcagcaca 34260 gcaccacaat attgttcaaa atcccacagt gcaaggcgct gtatccaaag ctcatggcgg 34320 ggaccacaga acccacgtgg ccatcatacc acaagcgcag gtagattaag tggcgacccc 34380 tcataaacac gctggacata aacattacct cttttggcat gttgtaattc accacctccc 34440 ggtaccatat aaacctctga ttaaacatgg cgccatccac caccatccta aaccagctgg 34500 ccaaaacctg cccgccggct atacactgca gggaaccggg actggaacaa tgacagtgga 34560 gagcccagga ctcgtaacca tggatcatca tgctcgtcat gatatcaatg ttggcacaac 34620 acaggcacac gtgcatacac ttcctcagga ttacaagctc ctcccgcgtt agaaccatat 34680 cccagggaac aacccattcc tgaatcagcg taaatcccac actgcaggga agacctcgca 34740 cgtaactcac gttgtgcatt gtcaaagtgt tacattcggg cagcagcgga tgatcctcca 34800 gtatggtagc gcgggtttct gtctcaaaag gaggtagacg atccctactg tacggagtgc 34860 gccgagacaa ccgagatcgt gttggtcgta gtgtcatgcc aaatggaacg ccggacgtag 34920 tcatatttcc tgaagcaaaa ccaggtgcgg gcgtgacaaa cagatctgcg tctccggtct 34980 cgccgcttag atcgctctgt gtagtagttg tagtatatcc actctctcaa agcatccagg 35040 cgccccctgg cttcgggttc tatgtaaact ccttcatgcg ccgctgccct gataacatcc 35100 accaccgcag aataagccac acccagccaa cctacacatt cgttctgcga gtcacacacg 35160 ggaggagcgg gaagagctgg aagaaccatg tttttttttt tattccaaaa gattatccaa 35220 aacctcaaaa tgaagatcta ttaagtgaac gcgctcccct ccggtggcgt ggtcaaactc 35280 tacagccaaa gaacagataa tggcatttgt aagatgttgc acaatggctt ccaaaaggca 35340 aacggccctc acgtccaagt ggacgtaaag gctaaaccct tcagggtgaa tctcctctat 35400 aaacattcca gcaccttcaa ccatgcccaa ataattctca tctcgccacc ttctcaatat 35460 atctctaagc aaatcccgaa tattaagtcc ggccattgta aaaatctgct ccagagcgcc 35520 ctccaccttc agcctcaagc agcgaatcat gattgcaaaa attcaggttc ctcacagacc 35580 tgtataagat tcaaaagcgg aacattaaca aaaataccgc gatcccgtag gtcccttcgc 35640 agggccagct gaacataatc gtgcaggtct gcacggacca gcgcggccac ttccccgcca 35700 ggaaccatga caaaagaacc cacactgatt atgacacgca tactcggagc tatgctaacc 35760 agcgtagccc cgatgtaagc ttgttgcatg ggcggcgata taaaatgcaa ggtgctgctc 35820 aaaaaatcag gcaaagcctc gcgcaaaaaa gaaagcacat cgtagtcatg ctcatgcaga 35880 taaaggcagg taagctccgg aaccaccaca gaaaaagaca ccatttttct ctcaaacatg 35940 tctgcgggtt tctgcataaa cacaaaataa aataacaaaa aaacatttaa acattagaag 36000 cctgtcttac aacaggaaaa acaaccctta taagcataag acggactacg gccatgccgg 36060 cgtgaccgta aaaaaactgg tcaccgtgat taaaaagcac caccgacagc tcctcggtca 36120 tgtccggagt cataatgtaa gactcggtaa acacatcagg ttgattcaca tcggtcagtg 36180 ctaaaaagcg accgaaatag cccgggggaa tacatacccg caggcgtaga gacaacatta 36240 cagcccccat aggaggtata acaaaattaa taggagagaa aaacacataa acacctgaaa 36300 aaccctcctg cctaggcaaa atagcaccct cccgctccag aacaacatac agcgcttcca 36360 cagcggcagc cataacagtc agccttacca gtaaaaaaga aaacctatta aaaaaacacc 36420 actcgacacg gcaccagctc aatcagtcac agtgtaaaaa agggccaagt gcagagcgag 36480 tatatatagg actaaaaaat gacgtaacgg ttaaagtcca caaaaaacac ccagaaaacc 36540 gcacgcgaac ctacgcccag aaacgaaagc caaaaaaccc acaacttcct caaatcgtca 36600 cttccgtttt cccacgttac gtcacttccc attttaagaa aactacaatt cccaacacat 36660 acaagttact ccgccctaaa acctacgtca cccgccccgt tcccacgccc cgcgccacgt 36720 cacaaactcc accccctcat tatcatattg gcttcaatcc aaaataaggt atattattga 36780 tgatg 36785 <210> 7 <211> 36797 <212> DNA <213> Artificial Sequence <220> <223> Synthetic adenovirus genome YPet-P2A-ADP <400> 7 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420 cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480 tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540 tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600 aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660 tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720 cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780 gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840 cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900 ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960 cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020 caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080 ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140 tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200 gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260 ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320 cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380 ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440 gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500 cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560 ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620 gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680 cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740 ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800 tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860 gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920 caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980 gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040 agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100 aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160 cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220 gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280 gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340 gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400 gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460 tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520 ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580 tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640 agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700 tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760 gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820 acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880 gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940 aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000 ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060 gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120 tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180 acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240 aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300 tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360 gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420 tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480 ttggctctag cgatgaagat acagattgag gtactgaaat gtgtgggcgt ggcttaaggg 3540 tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg 3600 ccgccgccca acttttctat acaaagttgt aatgagcacc aactcgtttg atggaagcat 3660 tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat 3720 gggctccagc attgatggtc gccccgtcct gcccgcaaac tctactacct tgacctacga 3780 gaccgtgtct ggaacgccgt tggagactgc agcctccgcc gccgcttcag ccgctgcagc 3840 caccgcccgc gggattgtga ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc 3900 ttcccgttca tccgcccgcg atgacaagtt gacggctctt ttggcacaat tggattcttt 3960 gacccgggaa cttaatgtcg tttctcagca gctgttggat ctgcgccagc aggtttctgc 4020 cctgaaggct tcctcccctc ccaatgcggt ttaaaacata aataaaaaac cagactctgt 4080 ttggatttgg atcaagcata agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg 4140 taggcccggg accagcggtc tcggtcgttg agggtcctgt gtattttttc caggacgtgg 4200 taaaggtgac tctggatgtt cagatacatg ggcataagcc cgtctctggg gtggaggtag 4260 caccactgca gagcttcatg ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag 4320 cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc tgattgccag gggcaggccc 4380 ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt gcatacgtgg ggatatgaga 4440 tgcatcttgg actgtatttt taggttggct atgttcccag ccatatccct ccggggattc 4500 atgttgtgca gaaccaccag cacagtgtat ccggtgcact tgggaaattt gtcatgtagc 4560 ttagaaggaa atgcgtggaa gaacttggag acgcccttgt gacctccaag attttccatg 4620 cattcgtcca taatgatggc aatgggccca cgggcggcgg cctgggcgaa gatatttctg 4680 ggatcactaa cgtcatagtt gtgttccagg atgagatcgt cataggccat ttttacaaag 4740 cgcgggcgga gggtgccaga ctgcggtata atggttccat ccggcccagg ggcgtagtta 4800 ccctcacaga tttgcatttc ccacgctttg agttcagatg gggggatcat gtctacctgc 4860 ggggcgatga agaaaacggt ttccggggta ggggagatca gctgggaaga aagcaggttc 4920 ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa tcacacctat taccgggtgc 4980 aactggtagt taagagagct gcagctgccg tcatccctga gcaggggggc cacttcgtta 5040 agcatgtccc tgactcgcat gttttccctg accaaatccg ccagaaggcg ctcgccgccc 5100 agcgatagca gttcttgcaa ggaagcaaag tttttcaacg gtttgagacc gtccgccgta 5160 ggcatgcttt tgagcgtttg accaagcagt tccaggcggt cccacagctc ggtcacctgc 5220 tctacggcat ctcgatccag catatctcct cgtttcgcgg gttggggcgg ctttcgctgt 5280 acggcagtag tcggtgctcg tccagacggg ccagggtcat gtctttccac gggcgcaggg 5340 tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc tccgggctgc gcgctggcca 5400 gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt 5460 cggccaggta gcatttgacc atggtgtcat agtccagccc ctccgcggcg tggcccttgg 5520 cgcgcagctt gcccttggag gaggcgccgc acgaggggca gtgcagactt ttgagggcgt 5580 agagcttggg cgcgagaaat accgattccg gggagtaggc atccgcgccg caggccccgc 5640 agacggtctc gcattccacg agccaggtga gctctggccg ttcggggtca aaaaccaggt 5700 ttcccccatg ctttttgatg cgtttcttac ctctggtttc catgagccgg tgtccacgct 5760 cggtgacgaa aaggctgtcc gtgtccccgt atacagactt gagaggcctg tcctcgagcg 5820 gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacaaag gctcgcgtcc 5880 aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact agggggtcca 5940 ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag gtgattggtt 6000 tgtaggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag ggggtggggg 6060 cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt tggggtgagt 6120 actccctctg aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg 6180 aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgca tccatctggt 6240 cagaaaagac aatctttttg ttgtcaagct tggtggcaaa cgacccgtag agggcgttgg 6300 acagcaactt ggcgatggag cgcagggttt ggtttttgtc gcgatcggcg cgctccttgg 6360 ccgcgatgtt tagctgcacg tattcgcgcg caacgcaccg ccattcggga aagacggtgg 6420 tgcgctcgtc gggcaccagg tgcacgcgcc aaccgcggtt gtgcagggtg acaaggtcaa 6480 cgctggtggc tacctctccg cgtaggcgct cgttggtcca gcagaggcgg ccgcccttgc 6540 gcgagcagaa tggcggtagg gggtctagct gcgtctcgtc cggggggtct gcgtccacgg 6600 taaagacccc gggcagcagg cgcgcgtcga agtagtctat cttgcatcct tgcaagtcta 6660 gcgcctgctg ccatgcgcgg gcggcaagcg cgcgctcgta tgggttgagt gggggacccc 6720 atggcatggg gtgggtgagc gcggaggcgt acatgccgca aatgtcgtaa acgtagaggg 6780 gctctctgag tattccaaga tatgtagggt agcatcttcc accgcggatg ctggcgcgca 6840 cgtaatcgta tagttcgtgc gagggagcga ggaggtcggg accgaggttg ctacgggcgg 6900 gctgctctgc tcggaagact atctgcctga agatggcatg tgagttggat gatatggttg 6960 gacgctggaa gacgttgaag ctggcgtctg tgagacctac cgcgtcacgc acgaaggagg 7020 cgtaggagtc gcgcagcttg ttgaccagct cggcggtgac ctgcacgtct agggcgcagt 7080 agtccagggt ttccttgatg atgtcatact tatcctgtcc cttttttttc cacagctcgc 7140 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct 7200 ccgaacggta agagcctagc atgtagaact ggttgacggc ctggtaggcg cagcatccct 7260 tttctacggg tagcgcgtat gcctgcgcgg ccttccggag cgaggtgtgg gtgagcgcaa 7320 aggtgtccct gaccatgact ttgaggtact ggtatttgaa gtcagtgtcg tcgcatccgc 7380 cctgctccca gagcaaaaag tccgtgcgct ttttggaacg cggatttggc agggcgaagg 7440 tgacatcgtt gaagagtatc tttcccgcgc gaggcataaa gttgcgtgtg atgcggaagg 7500 gtcccggcac ctcggaacgg ttgttaatta cctgggcggc gagcacgatc tcgtcaaagc 7560 cgttgatgtt gtggcccaca atgtaaagtt ccaagaagcg cgggatgccc ttgatggaag 7620 gcaatttttt aagttcctcg taggtgagct cttcagggga gctgagcccg tgctctgaaa 7680 gggcccagtc tgcaagatga gggttggaag cgacgaatga gctccacagg tcacgggcca 7740 ttagcatttg caggtggtcg cgaaaggtcc taaactggcg acctatggcc attttttctg 7800 gggtgatgca gtagaaggta agcgggtctt gttcccagcg gtcccatcca aggttcgcgg 7860 ctaggtctcg cgcggcagtc actagaggct catctccgcc gaacttcatg accagcatga 7920 agggcacgag ctgcttccca aaggccccca tccaagtata ggtctctaca tcgtaggtga 7980 caaagagacg ctcggtgcga ggatgcgagc cgatcgggaa gaactggatc tcccgccacc 8040 aattggagga gtggctattg atgtggtgaa agtagaagtc cctgcgacgg gccgaacact 8100 cgtgctggct tttgtaaaaa cgtgcgcagt actggcagcg gtgcacgggc tgtacatcct 8160 gcacgaggtt gacctgacga ccgcgcacaa ggaagcagag tgggaatttg agcccctcgc 8220 ctggcgggtt tggctggtgg tcttctactt cggctgcttg tccttgaccg tctggctgct 8280 cgaggggagt tacggtggat cggaccacca cgccgcgcga gcccaaagtc cagatgtccg 8340 cgcgcggcgg tcggagcttg atgacaacat cgcgcagatg ggagctgtcc atggtctgga 8400 gctcccgcgg cgtcaggtca ggcgggagct cctgcaggtt tacctcgcat agacgggtca 8460 gggcgcgggc tagatccagg tgatacctaa tttccagggg ctggttggtg gcggcgtcga 8520 tggcttgcaa gaggccgcat ccccgcggcg cgactacggt accgcgcggc gggcggtggg 8580 ccgcgggggt gtccttggat gatgcatcta aaagcggtga cgcgggcgag cccccggagg 8640 tagggggggc tccggacccg ccgggagagg gggcaggggc acgtcggcgc cgcgcgcggg 8700 caggagctgg tgctgcgcgc gtaggttgct ggcgaacgcg acgacgcggc ggttgatctc 8760 ctgaatctgg cgcctctgcg tgaagacgac gggcccggtg agcttgagcc tgaaagagag 8820 ttcgacagaa tcaatttcgg tgtcgttgac ggcggcctgg cgcaaaatct cctgcacgtc 8880 tcctgagttg tcttgatagg cgatctcggc catgaactgc tcgatctctt cctcctggag 8940 atctccgcgt ccggctcgct ccacggtggc ggcgaggtcg ttggaaatgc gggccatgag 9000 ctgcgagaag gcgttgaggc ctccctcgtt ccagacgcgg ctgtagacca cgcccccttc 9060 ggcatcgcgg gcgcgcatga ccacctgcgc gagattgagc tccacgtgcc gggcgaagac 9120 ggcgtagttt cgcaggcgct gaaagaggta gttgagggtg gtggcggtgt gttctgccac 9180 gaagaagtac ataacccagc gtcgcaacgt ggattcgttg atatccccca aggcctcaag 9240 gcgctccatg gcctcgtaga agtccacggc gaagttgaaa aactgggagt tgcgcgccga 9300 cacggttaac tcctcctcca gaagacggat gagctcggcg acagtgtcgc gcacctcgcg 9360 ctcaaaggct acaggggcct cttcttcttc ttcaatctcc tcttccataa gggcctcccc 9420 ttcttcttct tctggcggcg gtgggggagg ggggacacgg cggcgacgac ggcgcaccgg 9480 gaggcggtcg acaaagcgct cgatcatctc cccgcggcga cggcgcatgg tctcggtgac 9540 ggcgcggccg ttctcgcggg ggcgcagttg gaagacgccg cccgtcatgt cccggttatg 9600 ggttggcggg gggctgccat gcggcaggga tacggcgcta acgatgcatc tcaacaattg 9660 ttgtgtaggt actccgccgc cgagggacct gagcgagtcc gcatcgaccg gatcggaaaa 9720 cctctcgaga aaggcgtcta accagtcaca gtcgcaaggt aggctgagca ccgtggcggg 9780 cggcagcggg cggcggtcgg ggttgtttct ggcggaggtg ctgctgatga tgtaattaaa 9840 gtaggcggtc ttgagacggc ggatggtcga cagaagcacc atgtccttgg gtccggcctg 9900 ctgaatgcgc aggcggtcgg ccatgcccca ggcttcgttt tgacatcggc gcaggtcttt 9960 gtagtagtct tgcatgagcc tttctaccgg cacttcttct tctccttcct cttgtcctgc 10020 atctcttgca tctatcgctg cggcggcggc ggagtttggc cgtaggtggc gccctcttcc 10080 tcccatgcgt gtgaccccga agcccctcat cggctgaagc agggctaggt cggcgacaac 10140 gcgctcggct aatatggcct gctgcacctg cgtgagggta gactggaagt catccatgtc 10200 cacaaagcgg tggtatgcgc ccgtgttgat ggtgtaagtg cagttggcca taacggacca 10260 gttaacggtc tggtgacccg gctgcgagag ctcggtgtac ctgagacgcg agtaagccct 10320 cgagtcaaat acgtagtcgt tgcaagtccg caccaggtac tggtatccca ccaaaaagtg 10380 cggcggcggc tggcggtaga ggggccagcg tagggtggcc ggggctccgg gggcgagatc 10440 ttccaacata aggcgatgat atccgtagat gtacctggac atccaggtga tgccggcggc 10500 ggtggtggag gcgcgcggaa agtcgcggac gcggttccag atgttgcgca gcggcaaaaa 10560 gtgctccatg gtcgggacgc tctggccggt caggcgcgcg caatcgttga cgctctagac 10620 cgtgcaaaag gagagcctgt aagcgggcac tcttccgtgg tctggtggat aaattcgcaa 10680 gggtatcatg gcggacgacc ggggttcgag ccccgtatcc ggccgtccgc cgtgatccat 10740 gcggttaccg cccgcgtgtc gaacccaggt gtgcgacgtc agacaacggg ggagtgctcc 10800 ttttggcttc cttccaggcg cggcggctgc tgcgctagct tttttggcca ctggccgcgc 10860 gcagcgtaag cggttaggct ggaaagcgaa agcattaagt ggctcgctcc ctgtagccgg 10920 agggttattt tccaagggtt gagtcgcggg acccccggtt cgagtctcgg accggccgga 10980 ctgcggcgaa cgggggtttg cctccccgtc atgcaagacc ccgcttgcaa attcctccgg 11040 aaacagggac gagccccttt tttgcttttc ccagatgcat ccggtgctgc ggcagatgcg 11100 cccccctcct cagcagcggc aagagcaaga gcagcggcag acatgcaggg caccctcccc 11160 tcctcctacc gcgtcaggag gggcgacatc cgcggttgac gcggcagcag atggtgatta 11220 cgaacccccg cggcgccggg cccggcacta cctggacttg gaggagggcg agggcctggc 11280 gcggctagga gcgccctctc ctgagcggta cccaagggtg cagctgaagc gtgatacgcg 11340 tgaggcgtac gtgccgcggc agaacctgtt tcgcgaccgc gagggagagg agcccgagga 11400 gatgcgggat cgaaagttcc acgcagggcg cgagctgcgg catggcctga atcgcgagcg 11460 gttgctgcgc gaggaggact ttgagcccga cgcgcgaacc gggattagtc ccgcgcgcgc 11520 acacgtggcg gccgccgacc tggtaaccgc atacgagcag acggtgaacc aggagattaa 11580 ctttcaaaaa agctttaaca accacgtgcg tacgcttgtg gcgcgcgagg aggtggctat 11640 aggactgatg catctgtggg actttgtaag cgcgctggag caaaacccaa atagcaagcc 11700 gctcatggcg cagctgttcc ttatagtgca gcacagcagg gacaacgagg cattcaggga 11760 tgcgctgcta aacatagtag agcccgaggg ccgctggctg ctcgatttga taaacatcct 11820 gcagagcata gtggtgcagg agcgcagctt gagcctggct gacaaggtgg ccgccatcaa 11880 ctattccatg cttagcctgg gcaagtttta cgcccgcaag atataccata ccccttacgt 11940 tcccatagac aaggaggtaa agatcgaggg gttctacatg cgcatggcgc tgaaggtgct 12000 taccttgagc gacgacctgg gcgtttatcg caacgagcgc atccacaagg ccgtgagcgt 12060 gagccggcgg cgcgagctca gcgaccgcga gctgatgcac agcctgcaaa gggccctggc 12120 tggcacgggc agcggcgata gagaggccga gtcctacttt gacgcgggcg ctgacctgcg 12180 ctgggcccca agccgacgcg ccctggaggc agctggggcc ggacctgggc tggcggtggc 12240 acccgcgcgc gctggcaacg tcggcggcgt ggaggaatat gacgaggacg atgagtacga 12300 gccagaggac ggcgagtact aagcggtgat gtttctgatc agatgatgca agacgcaacg 12360 gacccggcgg tgcgggcggc gctgcagagc cagccgtccg gccttaactc cacggacgac 12420 tggcgccagg tcatggaccg catcatgtcg ctgactgcgc gcaatcctga cgcgttccgg 12480 cagcagccgc aggccaaccg gctctccgca attctggaag cggtggtccc ggcgcgcgca 12540 aaccccacgc acgagaaggt gctggcgatc gtaaacgcgc tggccgaaaa cagggccatc 12600 cggcccgacg aggccggcct ggtctacgac gcgctgcttc agcgcgtggc tcgttacaac 12660 agcggcaacg tgcagaccaa cctggaccgg ctggtggggg atgtgcgcga ggccgtggcg 12720 cagcgtgagc gcgcgcagca gcagggcaac ctgggctcca tggttgcact aaacgccttc 12780 ctgagtacac agcccgccaa cgtgccgcgg ggacaggagg actacaccaa ctttgtgagc 12840 gcactgcggc taatggtgac tgagacaccg caaagtgagg tgtaccagtc tgggccagac 12900 tattttttcc agaccagtag acaaggcctg cagaccgtaa acctgagcca ggctttcaaa 12960 aacttgcagg ggctgtgggg ggtgcgggct cccacaggcg accgcgcgac cgtgtctagc 13020 ttgctgacgc ccaactcgcg cctgttgctg ctgctaatag cgcccttcac ggacagtggc 13080 agcgtgtccc gggacacata cctaggtcac ttgctgacac tgtaccgcga ggccataggt 13140 caggcgcatg tggacgagca tactttccag gagattacaa gtgtcagccg cgcgctgggg 13200 caggaggaca cgggcagcct ggaggcaacc ctaaactacc tgctgaccaa ccggcggcag 13260 aagatcccct cgttgcacag tttaaacagc gaggaggagc gcattttgcg ctacgtgcag 13320 cagagcgtga gccttaacct gatgcgcgac ggggtaacgc ccagcgtggc gctggacatg 13380 accgcgcgca acatggaacc gggcatgtat gcctcaaacc ggccgtttat caaccgccta 13440 atggactact tgcatcgcgc ggccgccgtg aaccccgagt atttcaccaa tgccatcttg 13500 aacccgcact ggctaccgcc ccctggtttc tacaccgggg gattcgaggt gcccgagggt 13560 aacgatggat tcctctggga cgacatagac gacagcgtgt tttccccgca accgcagacc 13620 ctgctagagt tgcaacagcg cgagcaggca gaggcggcgc tgcgaaagga aagcttccgc 13680 aggccaagca gcttgtccga tctaggcgct gcggccccgc ggtcagatgc tagtagccca 13740 tttccaagct tgatagggtc tcttaccagc actcgcacca cccgcccgcg cctgctgggc 13800 gaggaggagt acctaaacaa ctcgctgctg cagccgcagc gcgaaaaaaa cctgcctccg 13860 gcatttccca acaacgggat agagagccta gtggacaaga tgagtagatg gaagacgtac 13920 gcgcaggagc acagggacgt gccaggcccg cgcccgccca cccgtcgtca aaggcacgac 13980 cgtcagcggg gtctggtgtg ggaggacgat gactcggcag acgacagcag cgtcctggat 14040 ttgggaggga gtggcaaccc gtttgcgcac cttcgcccca ggctggggag aatgttttaa 14100 aaaaaaaaaa gcatgatgca aaataaaaaa ctcaccaagg ccatggcacc gagcgttggt 14160 tttcttgtat tccccttagt atgcggcgcg cggcgatgta tgaggaaggt cctcctccct 14220 cctacgagag tgtggtgagc gcggcgccag tggcggcggc gctgggttct cccttcgatg 14280 ctcccctgga cccgccgttt gtgcctccgc ggtacctgcg gcctaccggg gggagaaaca 14340 gcatccgtta ctctgagttg gcacccctat tcgacaccac ccgtgtgtac ctggtggaca 14400 acaagtcaac ggatgtggca tccctgaact accagaacga ccacagcaac tttctgacca 14460 cggtcattca aaacaatgac tacagcccgg gggaggcaag cacacagacc atcaatcttg 14520 acgaccggtc gcactggggc ggcgacctga aaaccatcct gcataccaac atgccaaatg 14580 tgaacgagtt catgtttacc aataagttta aggcgcgggt gatggtgtcg cgcttgccta 14640 ctaaggacaa tcaggtggag ctgaaatacg agtgggtgga gttcacgctg cccgagggca 14700 actactccga gaccatgacc atagacctta tgaacaacgc gatcgtggag cactacttga 14760 aagtgggcag acagaacggg gttctggaaa gcgacatcgg ggtaaagttt gacacccgca 14820 acttcagact ggggtttgac cccgtcactg gtcttgtcat gcctggggta tatacaaacg 14880 aagccttcca tccagacatc attttgctgc caggatgcgg ggtggacttc acccacagcc 14940 gcctgagcaa cttgttgggc atccgcaagc ggcaaccctt ccaggagggc tttaggatca 15000 cctacgatga tctggagggt ggtaacattc ccgcactgtt ggatgtggac gcctaccagg 15060 cgagcttgaa agatgacacc gaacagggcg ggggtggcgc aggcggcagc aacagcagtg 15120 gcagcggcgc ggaagagaac tccaacgcgg cagccgcggc aatgcagccg gtggaggaca 15180 tgaacgatca tgccattcgc ggcgacacct ttgccacacg ggctgaggag aagcgcgctg 15240 aggccgaagc agcggccgaa gctgccgccc ccgctgcgca acccgaggtc gagaagcctc 15300 agaagaaacc ggtgatcaaa cccctgacag aggacagcaa gaaacgcagt tacaacctaa 15360 taagcaatga cagcaccttc acccagtacc gcagctggta ccttgcatac aactacggcg 15420 accctcagac cggaatccgc tcatggaccc tgctttgcac tcctgacgta acctgcggct 15480 cggagcaggt ctactggtcg ttgccagaca tgatgcaaga ccccgtgacc ttccgctcca 15540 cgcgccagat cagcaacttt ccggtggtgg gcgccgagct gttgcccgtg cactccaaga 15600 gcttctacaa cgaccaggcc gtctactccc aactcatccg ccagtttacc tctctgaccc 15660 acgtgttcaa tcgctttccc gagaaccaga ttttggcgcg cccgccagcc cccaccatca 15720 ccaccgtcag tgaaaacgtt cctgctctca cagatcacgg gacgctaccg ctgcgcaaca 15780 gcatcggagg agtccagcga gtgaccatta ctgacgccag acgccgcacc tgcccctacg 15840 tttacaaggc cctgggcata gtctcgccgc gcgtcctatc gagccgcact ttttgagcaa 15900 gcatgtccat ccttatatcg cccagcaata acacaggctg gggcctgcgc ttcccaagca 15960 agatgtttgg cggggccaag aagcgctccg accaacaccc agtgcgcgtg cgcgggcact 16020 accgcgcgcc ctggggcgcg cacaaacgcg gccgcactgg gcgcaccacc gtcgatgacg 16080 ccatcgacgc ggtggtggag gaggcgcgca actacacgcc cacgccgcca ccagtgtcca 16140 cagtggacgc ggccattcag accgtggtgc gcggagcccg gcgctatgct aaaatgaaga 16200 gacggcggag gcgcgtagca cgtcgccacc gccgccgacc cggcactgcc gcccaacgcg 16260 cggcggcggc cctgcttaac cgcgcacgtc gcaccggccg acgggcggcc atgcgggccg 16320 ctcgaaggct ggccgcgggt attgtcactg tgccccccag gtccaggcga cgagcggccg 16380 ccgcagcagc cgcggccatt agtgctatga ctcagggtcg caggggcaac gtgtattggg 16440 tgcgcgactc ggttagcggc ctgcgcgtgc ccgtgcgcac ccgccccccg cgcaactaga 16500 ttgcaagaaa aaactactta gactcgtact gttgtatgta tccagcggcg gcggcgcgca 16560 acgaagctat gtccaagcgc aaaatcaaag aagagatgct ccaggtcatc gcgccggaga 16620 tctatggccc cccgaagaag gaagagcagg attacaagcc ccgaaagcta aagcgggtca 16680 aaaagaaaaa gaaagatgat gatgatgaac ttgacgacga ggtggaactg ctgcacgcta 16740 ccgcgcccag gcgacgggta cagtggaaag gtcgacgcgt aaaacgtgtt ttgcgacccg 16800 gcaccaccgt agtctttacg cccggtgagc gctccacccg cacctacaag cgcgtgtatg 16860 atgaggtgta cggcgacgag gacctgcttg agcaggccaa cgagcgcctc ggggagtttg 16920 cctacggaaa gcggcataag gacatgctgg cgttgccgct ggacgagggc aacccaacac 16980 ctagcctaaa gcccgtaaca ctgcagcagg tgctgcccgc gcttgcaccg tccgaagaaa 17040 agcgcggcct aaagcgcgag tctggtgact tggcacccac cgtgcagctg atggtaccca 17100 agcgccagcg actggaagat gtcttggaaa aaatgaccgt ggaacctggg ctggagcccg 17160 aggtccgcgt gcggccaatc aagcaggtgg cgccgggact gggcgtgcag accgtggacg 17220 ttcagatacc cactaccagt agcaccagta ttgccaccgc cacagagggc atggagacac 17280 aaacgtcccc ggttgcctca gcggtggcgg atgccgcggt gcaggcggtc gctgcggccg 17340 cgtccaagac ctctacggag gtgcaaacgg acccgtggat gtttcgcgtt tcagcccccc 17400 ggcgcccgcg cggttcgagg aagtacggcg ccgccagcgc gctactgccc gaatatgccc 17460 tacatccttc cattgcgcct acccccggct atcgtggcta cacctaccgc cccagaagac 17520 gagcaactac ccgacgccga accaccactg gaacccgccg ccgccgtcgc cgtcgccagc 17580 ccgtgctggc cccgatttcc gtgcgcaggg tggctcgcga aggaggcagg accctggtgc 17640 tgccaacagc gcgctaccac cccagcatcg tttaaaagcc ggtctttgtg gttcttgcag 17700 atatggccct cacctgccgc ctccgtttcc cggtgccggg attccgagga agaatgcacc 17760 gtaggagggg catggccggc cacggcctga cgggcggcat gcgtcgtgcg caccaccggc 17820 ggcggcgcgc gtcgcaccgt cgcatgcgcg gcggtatcct gcccctcctt attccactga 17880 tcgccgcggc gattggcgcc gtgcccggaa ttgcatccgt ggccttgcag gcgcagagac 17940 actgattaaa aacaagttgc atgtggaaaa atcaaaataa aaagtctgga ctctcacgct 18000 cgcttggtcc tgtaactatt ttgtagaatg gaagacatca actttgcgtc tctggccccg 18060 cgacacggct cgcgcccgtt catgggaaac tggcaagata tcggcaccag caatatgagc 18120 ggtggcgcct tcagctgggg ctcgctgtgg agcggcatta aaaatttcgg ttccaccgtt 18180 aagaactatg gcagcaaggc ctggaacagc agcacaggcc agatgctgag ggataagttg 18240 aaagagcaaa atttccaaca aaaggtggta gatggcctgg cctctggcat tagcggggtg 18300 gtggacctgg ccaaccaggc agtgcaaaat aagattaaca gtaagcttga tccccgccct 18360 cccgtagagg agcctccacc ggccgtggag acagtgtctc cagaggggcg tggcgaaaag 18420 cgtccgcgcc ccgacaggga agaaactctg gtgacgcaaa tagacgagcc tccctcgtac 18480 gaggaggcac taaagcaagg cctgcccacc acccgtccca tcgcgcccat ggctaccgga 18540 gtgctgggcc agcacacacc cgtaacgctg gacctgcctc cccccgccga cacccagcag 18600 aaacctgtgc tgccaggccc gaccgccgtt gttgtaaccc gtcctagccg cgcgtccctg 18660 cgccgcgccg ccagcggtcc gcgatcgttg cggcccgtag ccagtggcaa ctggcaaagc 18720 acactgaaca gcatcgtggg tctgggggtg caatccctga agcgccgacg atgcttctga 18780 atagctaacg tgtcgtatgt gtgtcatgta tgcgtccatg tcgccgccag aggagctgct 18840 gagccgccgc gcgcccgctt tccaagatgg ctaccccttc gatgatgccg cagtggtctt 18900 acatgcacat ctcgggccag gacgcctcgg agtacctgag ccccgggctg gtgcagtttg 18960 cccgcgccac cgagacgtac ttcagcctga ataacaagtt tagaaacccc acggtggcgc 19020 ctacgcacga cgtgaccaca gaccggtccc agcgtttgac gctgcggttc atccctgtgg 19080 accgtgagga tactgcgtac tcgtacaagg cgcggttcac cctagctgtg ggtgataacc 19140 gtgtgctgga catggcttcc acgtactttg acatccgcgg cgtgctggac aggggcccta 19200 cttttaagcc ctactctggc actgcctaca acgccctggc tcccaagggt gccccaaatc 19260 cttgcgaatg ggatgaagct gctactgctc ttgaaataaa cctagaagaa gaggacgatg 19320 acaacgaaga cgaagtagac gagcaagctg agcagcaaaa aactcacgta tttgggcagg 19380 cgccttattc tggtataaat attacaaagg agggtattca aataggtgtc gaaggtcaaa 19440 cacctaaata tgccgataaa acatttcaac ctgaacctca aataggagaa tctcagtggt 19500 acgaaactga aattaatcat gcagctggga gagtccttaa aaagactacc ccaatgaaac 19560 catgttacgg ttcatatgca aaacccacaa atgaaaatgg agggcaaggc attcttgtaa 19620 agcaacaaaa tggaaagcta gaaagtcaag tggaaatgca atttttctca actactgagg 19680 cgaccgcagg caatggtgat aacttgactc ctaaagtggt attgtacagt gaagatgtag 19740 atatagaaac cccagacact catatttctt acatgcccac tattaaggaa ggtaactcac 19800 gagaactaat gggccaacaa tctatgccca acaggcctaa ttacattgct tttagggaca 19860 attttattgg tctaatgtat tacaacagca cgggtaatat gggtgttctg gcgggccaag 19920 catcgcagtt gaatgctgtt gtagatttgc aagacagaaa cacagagctt tcataccagc 19980 ttttgcttga ttccattggt gatagaacca ggtacttttc tatgtggaat caggctgttg 20040 acagctatga tccagatgtt agaattattg aaaatcatgg aactgaagat gaacttccaa 20100 attactgctt tccactggga ggtgtgatta atacagagac tcttaccaag gtaaaaccta 20160 aaacaggtca ggaaaatgga tgggaaaaag atgctacaga attttcagat aaaaatgaaa 20220 taagagttgg aaataatttt gccatggaaa tcaatctaaa tgccaacctg tggagaaatt 20280 tcctgtactc caacatagcg ctgtatttgc ccgacaagct aaagtacagt ccttccaacg 20340 taaaaatttc tgataaccca aacacctacg actacatgaa caagcgagtg gtggctcccg 20400 ggttagtgga ctgctacatt aaccttggag cacgctggtc ccttgactat atggacaacg 20460 tcaacccatt taaccaccac cgcaatgctg gcctgcgcta ccgctcaatg ttgctgggca 20520 atggtcgcta tgtgcccttc cacatccagg tgcctcagaa gttctttgcc attaaaaacc 20580 tccttctcct gccgggctca tacacctacg agtggaactt caggaaggat gttaacatgg 20640 ttctgcagag ctccctagga aatgacctaa gggttgacgg agccagcatt aagtttgata 20700 gcatttgcct ttacgccacc ttcttcccca tggcccacaa caccgcctcc acgcttgagg 20760 ccatgcttag aaacgacacc aacgaccagt cctttaacga ctatctctcc gccgccaaca 20820 tgctctaccc tatacccgcc aacgctacca acgtgcccat atccatcccc tcccgcaact 20880 gggcggcttt ccgcggctgg gccttcacgc gccttaagac taaggaaacc ccatcactgg 20940 gctcgggcta cgacccttat tacacctact ctggctctat accctaccta gatggaacct 21000 tttacctcaa ccacaccttt aagaaggtgg ccattacctt tgactcttct gtcagctggc 21060 ctggcaatga ccgcctgctt acccccaacg agtttgaaat taagcgctca gttgacgggg 21120 agggttacaa cgttgcccag tgtaacatga ccaaagactg gttcctggta caaatgctag 21180 ctaactacaa cattggctac cagggcttct atatcccaga gagctacaag gaccgcatgt 21240 actccttctt tagaaacttc cagcccatga gccgtcaggt ggtggatgat actaaataca 21300 aggactacca acaggtgggc atcctacacc aacacaacaa ctctggattt gttggctacc 21360 ttgcccccac catgcgcgaa ggacaggcct accctgctaa cttcccctat ccgcttatag 21420 gcaagaccgc agttgacagc attacccaga aaaagtttct ttgcgatcgc accctttggc 21480 gcatcccatt ctccagtaac tttatgtcca tgggcgcact cacagacctg ggccaaaacc 21540 ttctctacgc caactccgcc cacgcgctag acatgacttt tgaggtggat cccatggacg 21600 agcccaccct tctttatgtt ttgtttgaag tctttgacgt ggtccgtgtg caccggccgc 21660 accgcggcgt catcgaaacc gtgtacctgc gcacgccctt ctcggccggc aacgccacaa 21720 cataaagaag caagcaacat caacaacagc tgccgccatg ggctccagtg agcaggaact 21780 gaaagccatt gtcaaagatc ttggttgtgg gccatatttt ttgggcacct atgacaagcg 21840 ctttccaggc tttgtttctc cacacaagct cgcctgcgcc atagtcaata cggccggtcg 21900 cgagactggg ggcgtacact ggatggcctt tgcctggaac ccgcactcaa aaacatgcta 21960 cctctttgag ccctttggct tttctgacca gcgactcaag caggtttacc agtttgagta 22020 cgagtcactc ctgcgccgta gcgccattgc ttcttccccc gaccgctgta taacgctgga 22080 aaagtccacc caaagcgtac aggggcccaa ctcggccgcc tgtggactat tctgctgcat 22140 gtttctccac gcctttgcca actggcccca aactcccatg gatcacaacc ccaccatgaa 22200 ccttattacc ggggtaccca actccatgct caacagtccc caggtacagc ccaccctgcg 22260 tcgcaaccag gaacagctct acagcttcct ggagcgccac tcgccctact tccgcagcca 22320 cagtgcgcag attaggagcg ccacttcttt ttgtcacttg aaaaacatgt aaaaataatg 22380 tactagagac actttcaata aaggcaaatg cttttatttg tacactctcg ggtgattatt 22440 tacccccacc cttgccgtct gcgccgttta aaaatcaaag gggttctgcc gcgcatcgct 22500 atgcgccact ggcagggaca cgttgcgata ctggtgttta gtgctccact taaactcagg 22560 cacaaccatc cgcggcagct cggtgaagtt ttcactccac aggctgcgca ccatcaccaa 22620 cgcgtttagc aggtcgggcg ccgatatctt gaagtcgcag ttggggcctc cgccctgcgc 22680 gcgcgagttg cgatacacag ggttgcagca ctggaacact atcagcgccg ggtggtgcac 22740 gctggccagc acgctcttgt cggagatcag atccgcgtcc aggtcctccg cgttgctcag 22800 ggcgaacgga gtcaactttg gtagctgcct tcccaaaaag ggcgcgtgcc caggctttga 22860 gttgcactcg caccgtagtg gcatcaaaag gtgaccgtgc ccggtctggg cgttaggata 22920 cagcgcctgc ataaaagcct tgatctgctt aaaagccacc tgagcctttg cgccttcaga 22980 gaagaacatg ccgcaagact tgccggaaaa ctgattggcc ggacaggccg cgtcgtgcac 23040 gcagcacctt gcgtcggtgt tggagatctg caccacattt cggccccacc ggttcttcac 23100 gatcttggcc ttgctagact gctccttcag cgcgcgctgc ccgttttcgc tcgtcacatc 23160 catttcaatc acgtgctcct tatttatcat aatgcttccg tgtagacact taagctcgcc 23220 ttcgatctca gcgcagcggt gcagccacaa cgcgcagccc gtgggctcgt gatgcttgta 23280 ggtcacctct gcaaacgact gcaggtacgc ctgcaggaat cgccccatca tcgtcacaaa 23340 ggtcttgttg ctggtgaagg tcagctgcaa cccgcggtgc tcctcgttca gccaggtctt 23400 gcatacggcc gccagagctt ccacttggtc aggcagtagt ttgaagttcg cctttagatc 23460 gttatccacg tggtacttgt ccatcagcgc gcgcgcagcc tccatgccct tctcccacgc 23520 agacacgatc ggcacactca gcgggttcat caccgtaatt tcactttccg cttcgctggg 23580 ctcttcctct tcctcttgcg tccgcatacc acgcgccact gggtcgtctt cattcagccg 23640 ccgcactgtg cgcttacctc ctttgccatg cttgattagc accggtgggt tgctgaaacc 23700 caccatttgt agcgccacat cttctctttc ttcctcgctg tccacgatta cctctggtga 23760 tggcgggcgc tcgggcttgg gagaagggcg cttctttttc ttcttgggcg caatggccaa 23820 atccgccgcc gaggtcgatg gccgcgggct gggtgtgcgc ggcaccagcg cgtcttgtga 23880 tgagtcttcc tcgtcctcgg actcgatacg ccgcctcatc cgcttttttg ggggcgcccg 23940 gggaggcggc ggcgacgggg acggggacga cacgtcctcc atggttgggg gacgtcgcgc 24000 cgcaccgcgt ccgcgctcgg gggtggtttc gcgctgctcc tcttcccgac tggccatttc 24060 cttctcctat aggcagaaaa agatcatgga gtcagtcgag aagaaggaca gcctaaccgc 24120 cccctctgag ttcgccacca ccgcctccac cgatgccgcc aacgcgccta ccaccttccc 24180 cgtcgaggca cccccgcttg aggaggagga agtgattatc gagcaggacc caggttttgt 24240 aagcgaagac gacgaggacc gctcagtacc aacagaggat aaaaagcaag accaggacaa 24300 cgcagaggca aacgaggaac aagtcgggcg gggggacgaa aggcatggcg actacctaga 24360 tgtgggagac gacgtgctgt tgaagcatct gcagcgccag tgcgccatta tctgcgacgc 24420 gttgcaagag cgcagcgatg tgcccctcgc catagcggat gtcagccttg cctacgaacg 24480 ccacctattc tcaccgcgcg taccccccaa acgccaagaa aacggcacat gcgagcccaa 24540 cccgcgcctc aacttctacc ccgtatttgc cgtgccagag gtgcttgcca cctatcacat 24600 ctttttccaa aactgcaaga tacccctatc ctgccgtgcc aaccgcagcc gagcggacaa 24660 gcagctggcc ttgcggcagg gcgctgtcat acctgatatc gcctcgctca acgaagtgcc 24720 aaaaatcttt gagggtcttg gacgcgacga gaagcgcgcg gcaaacgctc tgcaacagga 24780 aaacagcgaa aatgaaagtc actctggagt gttggtggaa ctcgagggtg acaacgcgcg 24840 cctagccgta ctaaaacgca gcatcgaggt cacccacttt gcctacccgg cacttaacct 24900 accccccaag gtcatgagca cagtcatgag tgagctgatc gtgcgccgtg cgcagcccct 24960 ggagagggat gcaaatttgc aagaacaaac agaggagggc ctacccgcag ttggcgacga 25020 gcagctagcg cgctggcttc aaacgcgcga gcctgccgac ttggaggagc gacgcaaact 25080 aatgatggcc gcagtgctcg ttaccgtgga gcttgagtgc atgcagcggt tctttgctga 25140 cccggagatg cagcgcaagc tagaggaaac attgcactac acctttcgac agggctacgt 25200 acgccaggcc tgcaagatct ccaacgtgga gctctgcaac ctggtctcct accttggaat 25260 tttgcacgaa aaccgccttg ggcaaaacgt gcttcattcc acgctcaagg gcgaggcgcg 25320 ccgcgactac gtccgcgact gcgtttactt atttctatgc tacacctggc agacggccat 25380 gggcgtttgg cagcagtgct tggaggagtg caacctcaag gagctgcaga aactgctaaa 25440 gcaaaacttg aaggacctat ggacggcctt caacgagcgc tccgtggccg cgcacctggc 25500 ggacatcatt ttccccgaac gcctgcttaa aaccctgcaa cagggtctgc cagacttcac 25560 cagtcaaagc atgttgcaga actttaggaa ctttatccta gagcgctcag gaatcttgcc 25620 cgccacctgc tgtgcacttc ctagcgactt tgtgcccatt aagtaccgcg aatgccctcc 25680 gccgctttgg ggccactgct accttctgca gctagccaac taccttgcct accactctga 25740 cataatggaa gacgtgagcg gtgacggtct actggagtgt cactgtcgct gcaacctatg 25800 caccccgcac cgctccctgg tttgcaattc gcagctgctt aacgaaagtc aaattatcgg 25860 tacctttgag ctgcagggtc cctcgcctga cgaaaagtcc gcggctccgg ggttgaaact 25920 cactccgggg ctgtggacgt cggcttacct tcgcaaattt gtacctgagg actaccacgc 25980 ccacgagatt aggttctacg aagaccaatc ccgcccgcca aatgcggagc ttaccgcctg 26040 cgtcattacc cagggccaca ttcttggcca attgcaagcc atcaacaaag cccgccaaga 26100 gtttctgcta cgaaagggac ggggggttta cttggacccc cagtccggcg aggagctcaa 26160 cccaatcccc ccgccgccgc agccctatca gcagcagccg cgggcccttg cttcccagga 26220 tggcacccaa aaagaagctg cagctgccgc cgccacccac ggacgaggag gaatactggg 26280 acagtcaggc agaggaggtt ttggacgagg aggaggagga catgatggaa gactgggaga 26340 gcctagacga ggaagcttcc gaggtcgaag aggtgtcaga cgaaacaccg tcaccctcgg 26400 tcgcattccc ctcgccggcg ccccagaaat cggcaaccgg ttccagcatg gctacaacct 26460 ccgctcctca ggcgccgccg gcactgcccg ttcgccgacc caaccgtaga tgggacacca 26520 ctggaaccag ggccggtaag tccaagcagc cgccgccgtt agcccaagag caacaacagc 26580 gccaaggcta ccgctcatgg cgcgggcaca agaacgccat agttgcttgc ttgcaagact 26640 gtgggggcaa catctccttc gcccgccgct ttcttctcta ccatcacggc gtggccttcc 26700 cccgtaacat cctgcattac taccgtcatc tctacagccc atactgcacc ggcggcagcg 26760 gcagcggcag caacagcagc ggccacacag aagcaaaggc gaccggatag caagactctg 26820 acaaagccca agaaatccac agcggcggca gcagcaggag gaggagcgct gcgtctggcg 26880 cccaacgaac ccgtatcgac ccgcgagctt agaaacagga tttttcccac tctgtatgct 26940 atatttcaac agagcagggg ccaagaacaa gagctgaaaa taaaaaacag gtctctgcga 27000 tccctcaccc gcagctgcct gtatcacaaa agcgaagatc agcttcggcg cacgctggaa 27060 gacgcggagg ctctcttcag taaatactgc gcgctgactc ttaaggacta gtttcgcgcc 27120 ctttctcaaa tttaagcgcg aaaactacgt catctccagc ggccacaccc ggcgccagca 27180 cctgtcgtca gcgccatttc aactttgtat acaaaagttg tgatgagcaa ggaaattccc 27240 acgccctaca tgtggagtta ccagccacaa atgggacttg cggctggagc tgcccaagac 27300 tactcaaccc gaataaacta catgagcgcg ggaccccaca tgatatcccg ggtcaacgga 27360 atacgcgccc accgaaaccg aattctcctg gaacaggcgg ctattaccac cacacctcgt 27420 aataacctta atccccgtag ttggcccgct gccctggtgt accaggaaag tcccgctccc 27480 accactgtgg tacttcccag agacgcccag gccgaagttc agatgactaa ctcaggggcg 27540 cagcttgcgg gcggctttcg tcacagggtg cggtcgcccg ggcagggtat aactcacctg 27600 acaatcagag ggcgaggtat tcagctcaac gacgagtcgg tgagctcctc gcttggtctc 27660 cgtccggacg ggacatttca gatcggcggc gccggccgct cttcattcac gcctcgtcag 27720 gcaatcctaa ctctgcagac ctcgtcctct gagccgcgct ctggaggcat tggaactctg 27780 caatttattg aggagtttgt gccatcggtc tactttaacc ccttctcggg acctcccggc 27840 cactatccgg atcaatttat tcctaacttt gacgcggtaa aggactcggc ggacggctac 27900 gactgaatgt taagtggaga ggcagagcaa ctgcgcctga aacacctggt ccactgtcgc 27960 cgccacaagt gctttgcccg cgactccggt gagttttgct actttgaatt gcccgaggat 28020 catatcgagg gcccggcgca cggcgtccgg cttaccgccc agggagagct tgcccgtagc 28080 ctgattcggg agtttaccca gcgccccctg ctagttgagc gggacagggg accctgtgtt 28140 ctcactgtga tttgcaactg tcctaaccct ggattacatc aagatctttg ttgccatctc 28200 tgtgctgagt ataataaata cagaaattaa aatatactgg ggctcctatc gccatcctgt 28260 aaacgccacc gtcttcaccc gcccaagcaa accaaggcga accttacctg gtacttttaa 28320 catctctccc tctgtgattt acaacagttt caacccagac ggagtgagtc tacgagagaa 28380 cctctccgag ctcagctact ccatcagaaa aaacaccacc ctccttacct gccgggaacg 28440 tacgagtgcg tcaccggccg ctgcaccaca cctaccgcct gaccgtaaac cagacttttt 28500 ccggacagac ctcaataact ctgtttacca gaacaggagg tgagcttaga aaacccttag 28560 ggtattaggc caaaggcgca gctactgtgg ggtttatgaa caattcaagc aactctacgg 28620 gctattctaa ttcaggtttc tctagaatcg gggttggggt tattctctgt cttgtgattc 28680 tctttattct tatactaacg cttctctgcc taaggctcgc cgcctgctgt gtgcacattt 28740 gcatttattg tcagcttttt aaacgctggg gtcgccaccc aagatgatta ggtacataat 28800 cctaggttta ctcacccttg cgtcagccca cggtaccacc caaaaggtgg attttaagga 28860 gccagcctgt aatgttacat tcgcagctga agctaatgag tgcaccactc ttataaaatg 28920 caccacagaa catgaaaagc tgcttattcg ccacaaaaac aaaattggca agtatgctgt 28980 ttatgctatt tggcagccag gtgacactac agagtataat gttacagttt tccagggtaa 29040 aagtcataaa acttttatgt atacttttcc attttatgaa atgtgcgaca ttaccatgta 29100 catgagcaaa cagtataagt tgtggccccc acaaaattgt gtggaaaaca ctggcacttt 29160 ctgctgcact gctatgctaa ttacagtgct cgctttggtc tgtaccctac tctatattaa 29220 atacaaaagc agacgcagct ttattgagga aaagaaaatg ccttaattta ctaagttaca 29280 aagctaatgt caccactaac tgctttactc gctgcttgca aaacaaattc aaaaagttag 29340 cattataatt agaataggat ttaaaccccc cggtcatttc ctgctcaata ccattcccct 29400 gaacaattga ctctatgtgg gatatgctcc agcgctacaa ccttgaagtc aggcttcctg 29460 gatgtcagca tctgactttg gccagcacct gtcccgcgga tttgttccag tccaactaca 29520 gcgacccacc ctaacagaga tggtgagcaa aggcgaagag ctgttcaccg gcgtggtgcc 29580 catcctggtg gagctggacg gcgacgtgaa cggccacaag ttcagcgtga gcggcgaggg 29640 cgagggcgac gccacctacg gcaagctgac cctgaagctg ctgtgcacca ccggcaagct 29700 gcccgtgccc tggcccaccc tggtgaccac cctgggctac ggcgtgcagt gcttcgcccg 29760 gtaccccgac cacatgaagc agcacgactt cttcaagagc gccatgcccg agggctacgt 29820 gcaggagcgg accatcttct tcaaggacga cggcaactac aagacccggg ccgaggtgaa 29880 gttcgagggc gacaccctgg tgaaccggat cgagctgaag ggcatcgact tcaaggagga 29940 cggcaacatc ctgggccaca agctggagta caactacaac agccacaacg tgtacatcac 30000 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccggcaca acatcgagga 30060 cggcggcgtg cagctggccg accactacca gcagaacacc cccatcggcg acggccccgt 30120 gctgctgccc gacaaccact acctgagcta ccagagcgcc ctgttcaagg accccaacga 30180 gaagcgggac cacatggtgc tgctggagtt cctgaccgcc gccggcatca ccgagggcat 30240 gaacgagctc tataagggaa gcggagctac taacttcagc ctgctgaagc aggctggtga 30300 cgtcgaggag aatcctggcc caatggtgat gaccaacaca accaacgcgg ccgccgctac 30360 cggacttaca tctaccacaa atacacccca agtttctgcc tttgtcaata actgggataa 30420 cttgggcatg tggtggttct ccatagcgct tatgtttgta tgccttatta ttatgtggct 30480 catctgctgc ctaaagcgca aacgcgcccg accacccatc tatagtccca tcattgtgct 30540 acacccaaac aatgatggaa tccatagatt ggacggactg aaacacatgt tcttttctct 30600 tacagtatga ttaaatgaga catgattcct cgagttttta tattactgac ccttgttgcg 30660 cttttttgtg cgtgctccac attggctgcg gtttctcaca tcgaagtaga ctgcattcca 30720 gccttcacag tctatttgct ttacggattt gtcaccctca cgctcatctg cagcctcatc 30780 actgtggtca tcgcctttat ccagtgcatt gactgggtct gtgtgcgctt tgcatatctc 30840 agacaccatc cccagtacag ggacaggact atagctgagc ttcttagaat tctttaatta 30900 tgaaatttac tgtgactttt ctgctgatta tttgcaccct atctgcgttt tgttccccga 30960 cctccaagcc tcaaagacat atatcatgca gattcactcg tatatggaat attccaagtt 31020 gctacaatga aaaaagcgat ctttccgaag cctggttata tgcaatcatc tctgttatgg 31080 tgttctgcag taccatctta gccctagcta tatatcccta ccttgacatt ggctggaacg 31140 caatagatgc catgaaccac ccaactttcc ccgcgcccgc tatgcttcca ctgcaacaag 31200 ttgttgccgg cggctttgtc ccagccaatc agcctcgccc accttctccc acccccactg 31260 aaatcagcta ctttaatcta acaggaggag atgactgaca ccctagatct agaaatggac 31320 ggaattatta cagagcagcg cctgctagaa agacgcaggg cagcggccga gcaacagcgc 31380 atgaatcaag agctccaaga catggttaac ttgcaccagt gcaaaagggg tatcttttgt 31440 ctggtaaagc aggccaaagt cacctacgac agtaatacca ccggacaccg ccttagctac 31500 aagttgccaa ccaagcgtca gaaattggtg gtcatggtgg gagaaaagcc cattaccata 31560 actcagcact cggtagaaac cgaaggctgc attcactcac cttgtcaagg acctgaggat 31620 ctctgcaccc ttattaagac cctgtgcggt ctcaaagatc ttattccctt taactaataa 31680 aaaaaaataa taaagcatca cttacttaaa atcagttagc aaatttctgt ccagtttatt 31740 cagcagcacc tccttgccct cctcccagct ctggtattgc agcttcctcc tggctgcaaa 31800 ctttctccac aatctaaatg gaatgtcagt ttcctcctgt tcctgtccat ccgcacccac 31860 tatcttcatg ttgttgcaga tgaagcgcgc aagaccgtct gaagatacct tcaaccccgt 31920 gtatccatat gacacggaaa ccggtcctcc aactgtgcct tttcttactc ctccctttgt 31980 atcccccaat gggtttcaag agagtccccc tggggtactc tctttgcgcc tatccgaacc 32040 tctagttacc tccaatggca tgcttgcgct caaaatgggc aacggcctct ctctggacga 32100 ggccggcaac cttacctccc aaaatgtaac cactgtgagc ccacctctca aaaaaaccaa 32160 gtcaaacata aacctggaaa tatctgcacc cctcacagtt acctcagaag ccctaactgt 32220 ggctgccgcc gcacctctaa tggtcgcggg caacacactc accatgcaat cacaggcccc 32280 gctaaccgtg cacgactcca aacttagcat tgccacccaa ggacccctca cagtgtcaga 32340 aggaaagcta gccctgcaaa catcaggccc cctcaccacc accgatagca gtacccttac 32400 tatcactgcc tcaccccctc taactactgc cactggtagc ttgggcattg acttgaaaga 32460 gcccatttat acacaaaatg gaaaactagg actaaagtac ggggctcctt tgcatgtaac 32520 agacgaccta aacactttga ccgtagcaac tggtccaggt gtgactatta ataatacttc 32580 cttgcaaact aaagttactg gagccttggg ttttgattca caaggcaata tgcaacttaa 32640 tgtagcagga ggactaagga ttgattctca aaacagacgc cttatacttg atgttagtta 32700 tccgtttgat gctcaaaacc aactaaatct aagactagga cagggccctc tttttataaa 32760 ctcagcccac aacttggata ttaactacaa caaaggcctt tacttgttta cagcttcaaa 32820 caattccaaa aagcttgagg ttaacctaag cactgccaag gggttgatgt ttgacgctac 32880 agccatagcc attaatgcag gagatgggct tgaatttggt tcacctaatg caccaaacac 32940 aaatcccctc aaaacaaaaa ttggccatgg cctagaattt gattcaaaca aggctatggt 33000 tcctaaacta ggaactggcc ttagttttga cagcacaggt gccattacag taggaaacaa 33060 aaataatgat aagctaactt tgtggaccac accagctcca tctcctaact gtagactaaa 33120 tgcagagaaa gatgctaaac tcactttggt cttaacaaaa tgtggcagtc aaatacttgc 33180 tacagtttca gttttggctg ttaaaggcag tttggctcca atatctggaa cagttcaaag 33240 tgctcatctt attataagat ttgacgaaaa tggagtgcta ctaaacaatt ccttcctgga 33300 cccagaatat tggaacttta gaaatggaga tcttactgaa ggcacagcct atacaaacgc 33360 tgttggattt atgcctaacc tatcagctta tccaaaatct cacggtaaaa ctgccaaaag 33420 taacattgtc agtcaagttt acttaaacgg agacaaaact aaacctgtaa cactaaccat 33480 tacactaaac ggtacacagg aaacaggaga cacaactcca agtgcatact ctatgtcatt 33540 ttcatgggac tggtctggcc acaactacat taatgaaata tttgccacat cctcttacac 33600 tttttcatac attgcccaag aataaagaat cgtttgtgtt atgtttcaac gtgtttattt 33660 ttcaattgca gaaaatttca agtcattttt cattcagtag tatagcccca ccaccacata 33720 gcttatacag atcaccgtac ctcaactttg tataataaag ttgtaatcaa actcacagaa 33780 ccctagtatt caacctgcca cctccctccc aacacacaga gtacacagtc ctttctcccc 33840 ggctggcctt aaaaagcatc atatcatggg taacagacat attcttaggt gttatattcc 33900 acacggtttc ctgtcgagcc aaacgctcat cagtgatatt aataaactcc ccgggcagct 33960 cacttaagtt catgtcgctg tccagctgct gagccacagg ctgctgtcca acttgcggtt 34020 gcttaacggg cggcgaagga gaagtccacg cctacatggg ggtagagtca taatcgtgca 34080 tcaggatagg gcggtggtgc tgcagcagcg cgcgaataaa ctgctgccgc cgccgctccg 34140 tcctgcagga atacaacatg gcagtggtct cctcagcgat gattcgcacc gcccgcagca 34200 taaggcgcct tgtcctccgg gcacagcagc gcaccctgat ctcacttaaa tcagcacagt 34260 aactgcagca cagcaccaca atattgttca aaatcccaca gtgcaaggcg ctgtatccaa 34320 agctcatggc ggggaccaca gaacccacgt ggccatcata ccacaagcgc aggtagatta 34380 agtggcgacc cctcataaac acgctggaca taaacattac ctcttttggc atgttgtaat 34440 tcaccacctc ccggtaccat ataaacctct gattaaacat ggcgccatcc accaccatcc 34500 taaaccagct ggccaaaacc tgcccgccgg ctatacactg cagggaaccg ggactggaac 34560 aatgacagtg gagagcccag gactcgtaac catggatcat catgctcgtc atgatatcaa 34620 tgttggcaca acacaggcac acgtgcatac acttcctcag gattacaagc tcctcccgcg 34680 ttagaaccat atcccaggga acaacccatt cctgaatcag cgtaaatccc acactgcagg 34740 gaagacctcg cacgtaactc acgttgtgca ttgtcaaagt gttacattcg ggcagcagcg 34800 gatgatcctc cagtatggta gcgcgggttt ctgtctcaaa aggaggtaga cgatccctac 34860 tgtacggagt gcgccgagac aaccgagatc gtgttggtcg tagtgtcatg ccaaatggaa 34920 cgccggacgt agtcatattt cctgaagcaa aaccaggtgc gggcgtgaca aacagatctg 34980 cgtctccggt ctcgccgctt agatcgctct gtgtagtagt tgtagtatat ccactctctc 35040 aaagcatcca ggcgccccct ggcttcgggt tctatgtaaa ctccttcatg cgccgctgcc 35100 ctgataacat ccaccaccgc agaataagcc acacccagcc aacctacaca ttcgttctgc 35160 gagtcacaca cgggaggagc gggaagagct ggaagaacca tgtttttttt tttattccaa 35220 aagattatcc aaaacctcaa aatgaagatc tattaagtga acgcgctccc ctccggtggc 35280 gtggtcaaac tctacagcca aagaacagat aatggcattt gtaagatgtt gcacaatggc 35340 ttccaaaagg caaacggccc tcacgtccaa gtggacgtaa aggctaaacc cttcagggtg 35400 aatctcctct ataaacattc cagcaccttc aaccatgccc aaataattct catctcgcca 35460 ccttctcaat atatctctaa gcaaatcccg aatattaagt ccggccattg taaaaatctg 35520 ctccagagcg ccctccacct tcagcctcaa gcagcgaatc atgattgcaa aaattcaggt 35580 tcctcacaga cctgtataag attcaaaagc ggaacattaa caaaaatacc gcgatcccgt 35640 aggtcccttc gcagggccag ctgaacataa tcgtgcaggt ctgcacggac cagcgcggcc 35700 acttccccgc caggaaccat gacaaaagaa cccacactga ttatgacacg catactcgga 35760 gctatgctaa ccagcgtagc cccgatgtaa gcttgttgca tgggcggcga tataaaatgc 35820 aaggtgctgc tcaaaaaatc aggcaaagcc tcgcgcaaaa aagaaagcac atcgtagtca 35880 tgctcatgca gataaaggca ggtaagctcc ggaaccacca cagaaaaaga caccattttt 35940 ctctcaaaca tgtctgcggg tttctgcata aacacaaaat aaaataacaa aaaaacattt 36000 aaacattaga agcctgtctt acaacaggaa aaacaaccct tataagcata agacggacta 36060 cggccatgcc ggcgtgaccg taaaaaaact ggtcaccgtg attaaaaagc accaccgaca 36120 gctcctcggt catgtccgga gtcataatgt aagactcggt aaacacatca ggttgattca 36180 catcggtcag tgctaaaaag cgaccgaaat agcccggggg aatacatacc cgcaggcgta 36240 gagacaacat tacagccccc ataggaggta taacaaaatt aataggagag aaaaacacat 36300 aaacacctga aaaaccctcc tgcctaggca aaatagcacc ctcccgctcc agaacaacat 36360 acagcgcttc cacagcggca gccataacag tcagccttac cagtaaaaaa gaaaacctat 36420 taaaaaaaca ccactcgaca cggcaccagc tcaatcagtc acagtgtaaa aaagggccaa 36480 gtgcagagcg agtatatata ggactaaaaa atgacgtaac ggttaaagtc cacaaaaaac 36540 acccagaaaa ccgcacgcga acctacgccc agaaacgaaa gccaaaaaac ccacaacttc 36600 ctcaaatcgt cacttccgtt ttcccacgtt acgtcacttc ccattttaag aaaactacaa 36660 ttcccaacac atacaagtta ctccgcccta aaacctacgt cacccgcccc gttcccacgc 36720 cccgcgccac gtcacaaact ccaccccctc attatcatat tggcttcaat ccaaaataag 36780 gtatattatt gatgatg 36797 <210> 8 <211> 36791 <212> DNA <213> Artificial Sequence <220> <223> Synthetic adenovirus genome ADP-P2A-YPet <400> 8 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420 cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480 tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540 tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600 aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660 tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720 cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780 gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840 cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900 ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960 cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020 caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080 ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140 tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200 gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260 ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320 cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380 ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440 gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500 cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560 ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620 gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680 cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740 ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800 tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860 gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920 caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980 gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040 agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100 aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160 cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220 gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280 gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340 gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400 gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460 tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520 ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580 tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640 agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700 tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760 gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820 acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880 gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940 aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000 ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060 gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120 tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180 acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240 aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300 tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360 gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420 tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480 ttggctctag cgatgaagat acagattgag gtactgaaat gtgtgggcgt ggcttaaggg 3540 tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg 3600 ccgccgccca acttttctat acaaagttgt aatgagcacc aactcgtttg atggaagcat 3660 tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat 3720 gggctccagc attgatggtc gccccgtcct gcccgcaaac tctactacct tgacctacga 3780 gaccgtgtct ggaacgccgt tggagactgc agcctccgcc gccgcttcag ccgctgcagc 3840 caccgcccgc gggattgtga ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc 3900 ttcccgttca tccgcccgcg atgacaagtt gacggctctt ttggcacaat tggattcttt 3960 gacccgggaa cttaatgtcg tttctcagca gctgttggat ctgcgccagc aggtttctgc 4020 cctgaaggct tcctcccctc ccaatgcggt ttaaaacata aataaaaaac cagactctgt 4080 ttggatttgg atcaagcata agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg 4140 taggcccggg accagcggtc tcggtcgttg agggtcctgt gtattttttc caggacgtgg 4200 taaaggtgac tctggatgtt cagatacatg ggcataagcc cgtctctggg gtggaggtag 4260 caccactgca gagcttcatg ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag 4320 cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc tgattgccag gggcaggccc 4380 ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt gcatacgtgg ggatatgaga 4440 tgcatcttgg actgtatttt taggttggct atgttcccag ccatatccct ccggggattc 4500 atgttgtgca gaaccaccag cacagtgtat ccggtgcact tgggaaattt gtcatgtagc 4560 ttagaaggaa atgcgtggaa gaacttggag acgcccttgt gacctccaag attttccatg 4620 cattcgtcca taatgatggc aatgggccca cgggcggcgg cctgggcgaa gatatttctg 4680 ggatcactaa cgtcatagtt gtgttccagg atgagatcgt cataggccat ttttacaaag 4740 cgcgggcgga gggtgccaga ctgcggtata atggttccat ccggcccagg ggcgtagtta 4800 ccctcacaga tttgcatttc ccacgctttg agttcagatg gggggatcat gtctacctgc 4860 ggggcgatga agaaaacggt ttccggggta ggggagatca gctgggaaga aagcaggttc 4920 ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa tcacacctat taccgggtgc 4980 aactggtagt taagagagct gcagctgccg tcatccctga gcaggggggc cacttcgtta 5040 agcatgtccc tgactcgcat gttttccctg accaaatccg ccagaaggcg ctcgccgccc 5100 agcgatagca gttcttgcaa ggaagcaaag tttttcaacg gtttgagacc gtccgccgta 5160 ggcatgcttt tgagcgtttg accaagcagt tccaggcggt cccacagctc ggtcacctgc 5220 tctacggcat ctcgatccag catatctcct cgtttcgcgg gttggggcgg ctttcgctgt 5280 acggcagtag tcggtgctcg tccagacggg ccagggtcat gtctttccac gggcgcaggg 5340 tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc tccgggctgc gcgctggcca 5400 gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt 5460 cggccaggta gcatttgacc atggtgtcat agtccagccc ctccgcggcg tggcccttgg 5520 cgcgcagctt gcccttggag gaggcgccgc acgaggggca gtgcagactt ttgagggcgt 5580 agagcttggg cgcgagaaat accgattccg gggagtaggc atccgcgccg caggccccgc 5640 agacggtctc gcattccacg agccaggtga gctctggccg ttcggggtca aaaaccaggt 5700 ttcccccatg ctttttgatg cgtttcttac ctctggtttc catgagccgg tgtccacgct 5760 cggtgacgaa aaggctgtcc gtgtccccgt atacagactt gagaggcctg tcctcgagcg 5820 gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacaaag gctcgcgtcc 5880 aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact agggggtcca 5940 ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag gtgattggtt 6000 tgtaggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag ggggtggggg 6060 cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt tggggtgagt 6120 actccctctg aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg 6180 aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgca tccatctggt 6240 cagaaaagac aatctttttg ttgtcaagct tggtggcaaa cgacccgtag agggcgttgg 6300 acagcaactt ggcgatggag cgcagggttt ggtttttgtc gcgatcggcg cgctccttgg 6360 ccgcgatgtt tagctgcacg tattcgcgcg caacgcaccg ccattcggga aagacggtgg 6420 tgcgctcgtc gggcaccagg tgcacgcgcc aaccgcggtt gtgcagggtg acaaggtcaa 6480 cgctggtggc tacctctccg cgtaggcgct cgttggtcca gcagaggcgg ccgcccttgc 6540 gcgagcagaa tggcggtagg gggtctagct gcgtctcgtc cggggggtct gcgtccacgg 6600 taaagacccc gggcagcagg cgcgcgtcga agtagtctat cttgcatcct tgcaagtcta 6660 gcgcctgctg ccatgcgcgg gcggcaagcg cgcgctcgta tgggttgagt gggggacccc 6720 atggcatggg gtgggtgagc gcggaggcgt acatgccgca aatgtcgtaa acgtagaggg 6780 gctctctgag tattccaaga tatgtagggt agcatcttcc accgcggatg ctggcgcgca 6840 cgtaatcgta tagttcgtgc gagggagcga ggaggtcggg accgaggttg ctacgggcgg 6900 gctgctctgc tcggaagact atctgcctga agatggcatg tgagttggat gatatggttg 6960 gacgctggaa gacgttgaag ctggcgtctg tgagacctac cgcgtcacgc acgaaggagg 7020 cgtaggagtc gcgcagcttg ttgaccagct cggcggtgac ctgcacgtct agggcgcagt 7080 agtccagggt ttccttgatg atgtcatact tatcctgtcc cttttttttc cacagctcgc 7140 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct 7200 ccgaacggta agagcctagc atgtagaact ggttgacggc ctggtaggcg cagcatccct 7260 tttctacggg tagcgcgtat gcctgcgcgg ccttccggag cgaggtgtgg gtgagcgcaa 7320 aggtgtccct gaccatgact ttgaggtact ggtatttgaa gtcagtgtcg tcgcatccgc 7380 cctgctccca gagcaaaaag tccgtgcgct ttttggaacg cggatttggc agggcgaagg 7440 tgacatcgtt gaagagtatc tttcccgcgc gaggcataaa gttgcgtgtg atgcggaagg 7500 gtcccggcac ctcggaacgg ttgttaatta cctgggcggc gagcacgatc tcgtcaaagc 7560 cgttgatgtt gtggcccaca atgtaaagtt ccaagaagcg cgggatgccc ttgatggaag 7620 gcaatttttt aagttcctcg taggtgagct cttcagggga gctgagcccg tgctctgaaa 7680 gggcccagtc tgcaagatga gggttggaag cgacgaatga gctccacagg tcacgggcca 7740 ttagcatttg caggtggtcg cgaaaggtcc taaactggcg acctatggcc attttttctg 7800 gggtgatgca gtagaaggta agcgggtctt gttcccagcg gtcccatcca aggttcgcgg 7860 ctaggtctcg cgcggcagtc actagaggct catctccgcc gaacttcatg accagcatga 7920 agggcacgag ctgcttccca aaggccccca tccaagtata ggtctctaca tcgtaggtga 7980 caaagagacg ctcggtgcga ggatgcgagc cgatcgggaa gaactggatc tcccgccacc 8040 aattggagga gtggctattg atgtggtgaa agtagaagtc cctgcgacgg gccgaacact 8100 cgtgctggct tttgtaaaaa cgtgcgcagt actggcagcg gtgcacgggc tgtacatcct 8160 gcacgaggtt gacctgacga ccgcgcacaa ggaagcagag tgggaatttg agcccctcgc 8220 ctggcgggtt tggctggtgg tcttctactt cggctgcttg tccttgaccg tctggctgct 8280 cgaggggagt tacggtggat cggaccacca cgccgcgcga gcccaaagtc cagatgtccg 8340 cgcgcggcgg tcggagcttg atgacaacat cgcgcagatg ggagctgtcc atggtctgga 8400 gctcccgcgg cgtcaggtca ggcgggagct cctgcaggtt tacctcgcat agacgggtca 8460 gggcgcgggc tagatccagg tgatacctaa tttccagggg ctggttggtg gcggcgtcga 8520 tggcttgcaa gaggccgcat ccccgcggcg cgactacggt accgcgcggc gggcggtggg 8580 ccgcgggggt gtccttggat gatgcatcta aaagcggtga cgcgggcgag cccccggagg 8640 tagggggggc tccggacccg ccgggagagg gggcaggggc acgtcggcgc cgcgcgcggg 8700 caggagctgg tgctgcgcgc gtaggttgct ggcgaacgcg acgacgcggc ggttgatctc 8760 ctgaatctgg cgcctctgcg tgaagacgac gggcccggtg agcttgagcc tgaaagagag 8820 ttcgacagaa tcaatttcgg tgtcgttgac ggcggcctgg cgcaaaatct cctgcacgtc 8880 tcctgagttg tcttgatagg cgatctcggc catgaactgc tcgatctctt cctcctggag 8940 atctccgcgt ccggctcgct ccacggtggc ggcgaggtcg ttggaaatgc gggccatgag 9000 ctgcgagaag gcgttgaggc ctccctcgtt ccagacgcgg ctgtagacca cgcccccttc 9060 ggcatcgcgg gcgcgcatga ccacctgcgc gagattgagc tccacgtgcc gggcgaagac 9120 ggcgtagttt cgcaggcgct gaaagaggta gttgagggtg gtggcggtgt gttctgccac 9180 gaagaagtac ataacccagc gtcgcaacgt ggattcgttg atatccccca aggcctcaag 9240 gcgctccatg gcctcgtaga agtccacggc gaagttgaaa aactgggagt tgcgcgccga 9300 cacggttaac tcctcctcca gaagacggat gagctcggcg acagtgtcgc gcacctcgcg 9360 ctcaaaggct acaggggcct cttcttcttc ttcaatctcc tcttccataa gggcctcccc 9420 ttcttcttct tctggcggcg gtgggggagg ggggacacgg cggcgacgac ggcgcaccgg 9480 gaggcggtcg acaaagcgct cgatcatctc cccgcggcga cggcgcatgg tctcggtgac 9540 ggcgcggccg ttctcgcggg ggcgcagttg gaagacgccg cccgtcatgt cccggttatg 9600 ggttggcggg gggctgccat gcggcaggga tacggcgcta acgatgcatc tcaacaattg 9660 ttgtgtaggt actccgccgc cgagggacct gagcgagtcc gcatcgaccg gatcggaaaa 9720 cctctcgaga aaggcgtcta accagtcaca gtcgcaaggt aggctgagca ccgtggcggg 9780 cggcagcggg cggcggtcgg ggttgtttct ggcggaggtg ctgctgatga tgtaattaaa 9840 gtaggcggtc ttgagacggc ggatggtcga cagaagcacc atgtccttgg gtccggcctg 9900 ctgaatgcgc aggcggtcgg ccatgcccca ggcttcgttt tgacatcggc gcaggtcttt 9960 gtagtagtct tgcatgagcc tttctaccgg cacttcttct tctccttcct cttgtcctgc 10020 atctcttgca tctatcgctg cggcggcggc ggagtttggc cgtaggtggc gccctcttcc 10080 tcccatgcgt gtgaccccga agcccctcat cggctgaagc agggctaggt cggcgacaac 10140 gcgctcggct aatatggcct gctgcacctg cgtgagggta gactggaagt catccatgtc 10200 cacaaagcgg tggtatgcgc ccgtgttgat ggtgtaagtg cagttggcca taacggacca 10260 gttaacggtc tggtgacccg gctgcgagag ctcggtgtac ctgagacgcg agtaagccct 10320 cgagtcaaat acgtagtcgt tgcaagtccg caccaggtac tggtatccca ccaaaaagtg 10380 cggcggcggc tggcggtaga ggggccagcg tagggtggcc ggggctccgg gggcgagatc 10440 ttccaacata aggcgatgat atccgtagat gtacctggac atccaggtga tgccggcggc 10500 ggtggtggag gcgcgcggaa agtcgcggac gcggttccag atgttgcgca gcggcaaaaa 10560 gtgctccatg gtcgggacgc tctggccggt caggcgcgcg caatcgttga cgctctagac 10620 cgtgcaaaag gagagcctgt aagcgggcac tcttccgtgg tctggtggat aaattcgcaa 10680 gggtatcatg gcggacgacc ggggttcgag ccccgtatcc ggccgtccgc cgtgatccat 10740 gcggttaccg cccgcgtgtc gaacccaggt gtgcgacgtc agacaacggg ggagtgctcc 10800 ttttggcttc cttccaggcg cggcggctgc tgcgctagct tttttggcca ctggccgcgc 10860 gcagcgtaag cggttaggct ggaaagcgaa agcattaagt ggctcgctcc ctgtagccgg 10920 agggttattt tccaagggtt gagtcgcggg acccccggtt cgagtctcgg accggccgga 10980 ctgcggcgaa cgggggtttg cctccccgtc atgcaagacc ccgcttgcaa attcctccgg 11040 aaacagggac gagccccttt tttgcttttc ccagatgcat ccggtgctgc ggcagatgcg 11100 cccccctcct cagcagcggc aagagcaaga gcagcggcag acatgcaggg caccctcccc 11160 tcctcctacc gcgtcaggag gggcgacatc cgcggttgac gcggcagcag atggtgatta 11220 cgaacccccg cggcgccggg cccggcacta cctggacttg gaggagggcg agggcctggc 11280 gcggctagga gcgccctctc ctgagcggta cccaagggtg cagctgaagc gtgatacgcg 11340 tgaggcgtac gtgccgcggc agaacctgtt tcgcgaccgc gagggagagg agcccgagga 11400 gatgcgggat cgaaagttcc acgcagggcg cgagctgcgg catggcctga atcgcgagcg 11460 gttgctgcgc gaggaggact ttgagcccga cgcgcgaacc gggattagtc ccgcgcgcgc 11520 acacgtggcg gccgccgacc tggtaaccgc atacgagcag acggtgaacc aggagattaa 11580 ctttcaaaaa agctttaaca accacgtgcg tacgcttgtg gcgcgcgagg aggtggctat 11640 aggactgatg catctgtggg actttgtaag cgcgctggag caaaacccaa atagcaagcc 11700 gctcatggcg cagctgttcc ttatagtgca gcacagcagg gacaacgagg cattcaggga 11760 tgcgctgcta aacatagtag agcccgaggg ccgctggctg ctcgatttga taaacatcct 11820 gcagagcata gtggtgcagg agcgcagctt gagcctggct gacaaggtgg ccgccatcaa 11880 ctattccatg cttagcctgg gcaagtttta cgcccgcaag atataccata ccccttacgt 11940 tcccatagac aaggaggtaa agatcgaggg gttctacatg cgcatggcgc tgaaggtgct 12000 taccttgagc gacgacctgg gcgtttatcg caacgagcgc atccacaagg ccgtgagcgt 12060 gagccggcgg cgcgagctca gcgaccgcga gctgatgcac agcctgcaaa gggccctggc 12120 tggcacgggc agcggcgata gagaggccga gtcctacttt gacgcgggcg ctgacctgcg 12180 ctgggcccca agccgacgcg ccctggaggc agctggggcc ggacctgggc tggcggtggc 12240 acccgcgcgc gctggcaacg tcggcggcgt ggaggaatat gacgaggacg atgagtacga 12300 gccagaggac ggcgagtact aagcggtgat gtttctgatc agatgatgca agacgcaacg 12360 gacccggcgg tgcgggcggc gctgcagagc cagccgtccg gccttaactc cacggacgac 12420 tggcgccagg tcatggaccg catcatgtcg ctgactgcgc gcaatcctga cgcgttccgg 12480 cagcagccgc aggccaaccg gctctccgca attctggaag cggtggtccc ggcgcgcgca 12540 aaccccacgc acgagaaggt gctggcgatc gtaaacgcgc tggccgaaaa cagggccatc 12600 cggcccgacg aggccggcct ggtctacgac gcgctgcttc agcgcgtggc tcgttacaac 12660 agcggcaacg tgcagaccaa cctggaccgg ctggtggggg atgtgcgcga ggccgtggcg 12720 cagcgtgagc gcgcgcagca gcagggcaac ctgggctcca tggttgcact aaacgccttc 12780 ctgagtacac agcccgccaa cgtgccgcgg ggacaggagg actacaccaa ctttgtgagc 12840 gcactgcggc taatggtgac tgagacaccg caaagtgagg tgtaccagtc tgggccagac 12900 tattttttcc agaccagtag acaaggcctg cagaccgtaa acctgagcca ggctttcaaa 12960 aacttgcagg ggctgtgggg ggtgcgggct cccacaggcg accgcgcgac cgtgtctagc 13020 ttgctgacgc ccaactcgcg cctgttgctg ctgctaatag cgcccttcac ggacagtggc 13080 agcgtgtccc gggacacata cctaggtcac ttgctgacac tgtaccgcga ggccataggt 13140 caggcgcatg tggacgagca tactttccag gagattacaa gtgtcagccg cgcgctgggg 13200 caggaggaca cgggcagcct ggaggcaacc ctaaactacc tgctgaccaa ccggcggcag 13260 aagatcccct cgttgcacag tttaaacagc gaggaggagc gcattttgcg ctacgtgcag 13320 cagagcgtga gccttaacct gatgcgcgac ggggtaacgc ccagcgtggc gctggacatg 13380 accgcgcgca acatggaacc gggcatgtat gcctcaaacc ggccgtttat caaccgccta 13440 atggactact tgcatcgcgc ggccgccgtg aaccccgagt atttcaccaa tgccatcttg 13500 aacccgcact ggctaccgcc ccctggtttc tacaccgggg gattcgaggt gcccgagggt 13560 aacgatggat tcctctggga cgacatagac gacagcgtgt tttccccgca accgcagacc 13620 ctgctagagt tgcaacagcg cgagcaggca gaggcggcgc tgcgaaagga aagcttccgc 13680 aggccaagca gcttgtccga tctaggcgct gcggccccgc ggtcagatgc tagtagccca 13740 tttccaagct tgatagggtc tcttaccagc actcgcacca cccgcccgcg cctgctgggc 13800 gaggaggagt acctaaacaa ctcgctgctg cagccgcagc gcgaaaaaaa cctgcctccg 13860 gcatttccca acaacgggat agagagccta gtggacaaga tgagtagatg gaagacgtac 13920 gcgcaggagc acagggacgt gccaggcccg cgcccgccca cccgtcgtca aaggcacgac 13980 cgtcagcggg gtctggtgtg ggaggacgat gactcggcag acgacagcag cgtcctggat 14040 ttgggaggga gtggcaaccc gtttgcgcac cttcgcccca ggctggggag aatgttttaa 14100 aaaaaaaaaa gcatgatgca aaataaaaaa ctcaccaagg ccatggcacc gagcgttggt 14160 tttcttgtat tccccttagt atgcggcgcg cggcgatgta tgaggaaggt cctcctccct 14220 cctacgagag tgtggtgagc gcggcgccag tggcggcggc gctgggttct cccttcgatg 14280 ctcccctgga cccgccgttt gtgcctccgc ggtacctgcg gcctaccggg gggagaaaca 14340 gcatccgtta ctctgagttg gcacccctat tcgacaccac ccgtgtgtac ctggtggaca 14400 acaagtcaac ggatgtggca tccctgaact accagaacga ccacagcaac tttctgacca 14460 cggtcattca aaacaatgac tacagcccgg gggaggcaag cacacagacc atcaatcttg 14520 acgaccggtc gcactggggc ggcgacctga aaaccatcct gcataccaac atgccaaatg 14580 tgaacgagtt catgtttacc aataagttta aggcgcgggt gatggtgtcg cgcttgccta 14640 ctaaggacaa tcaggtggag ctgaaatacg agtgggtgga gttcacgctg cccgagggca 14700 actactccga gaccatgacc atagacctta tgaacaacgc gatcgtggag cactacttga 14760 aagtgggcag acagaacggg gttctggaaa gcgacatcgg ggtaaagttt gacacccgca 14820 acttcagact ggggtttgac cccgtcactg gtcttgtcat gcctggggta tatacaaacg 14880 aagccttcca tccagacatc attttgctgc caggatgcgg ggtggacttc acccacagcc 14940 gcctgagcaa cttgttgggc atccgcaagc ggcaaccctt ccaggagggc tttaggatca 15000 cctacgatga tctggagggt ggtaacattc ccgcactgtt ggatgtggac gcctaccagg 15060 cgagcttgaa agatgacacc gaacagggcg ggggtggcgc aggcggcagc aacagcagtg 15120 gcagcggcgc ggaagagaac tccaacgcgg cagccgcggc aatgcagccg gtggaggaca 15180 tgaacgatca tgccattcgc ggcgacacct ttgccacacg ggctgaggag aagcgcgctg 15240 aggccgaagc agcggccgaa gctgccgccc ccgctgcgca acccgaggtc gagaagcctc 15300 agaagaaacc ggtgatcaaa cccctgacag aggacagcaa gaaacgcagt tacaacctaa 15360 taagcaatga cagcaccttc acccagtacc gcagctggta ccttgcatac aactacggcg 15420 accctcagac cggaatccgc tcatggaccc tgctttgcac tcctgacgta acctgcggct 15480 cggagcaggt ctactggtcg ttgccagaca tgatgcaaga ccccgtgacc ttccgctcca 15540 cgcgccagat cagcaacttt ccggtggtgg gcgccgagct gttgcccgtg cactccaaga 15600 gcttctacaa cgaccaggcc gtctactccc aactcatccg ccagtttacc tctctgaccc 15660 acgtgttcaa tcgctttccc gagaaccaga ttttggcgcg cccgccagcc cccaccatca 15720 ccaccgtcag tgaaaacgtt cctgctctca cagatcacgg gacgctaccg ctgcgcaaca 15780 gcatcggagg agtccagcga gtgaccatta ctgacgccag acgccgcacc tgcccctacg 15840 tttacaaggc cctgggcata gtctcgccgc gcgtcctatc gagccgcact ttttgagcaa 15900 gcatgtccat ccttatatcg cccagcaata acacaggctg gggcctgcgc ttcccaagca 15960 agatgtttgg cggggccaag aagcgctccg accaacaccc agtgcgcgtg cgcgggcact 16020 accgcgcgcc ctggggcgcg cacaaacgcg gccgcactgg gcgcaccacc gtcgatgacg 16080 ccatcgacgc ggtggtggag gaggcgcgca actacacgcc cacgccgcca ccagtgtcca 16140 cagtggacgc ggccattcag accgtggtgc gcggagcccg gcgctatgct aaaatgaaga 16200 gacggcggag gcgcgtagca cgtcgccacc gccgccgacc cggcactgcc gcccaacgcg 16260 cggcggcggc cctgcttaac cgcgcacgtc gcaccggccg acgggcggcc atgcgggccg 16320 ctcgaaggct ggccgcgggt attgtcactg tgccccccag gtccaggcga cgagcggccg 16380 ccgcagcagc cgcggccatt agtgctatga ctcagggtcg caggggcaac gtgtattggg 16440 tgcgcgactc ggttagcggc ctgcgcgtgc ccgtgcgcac ccgccccccg cgcaactaga 16500 ttgcaagaaa aaactactta gactcgtact gttgtatgta tccagcggcg gcggcgcgca 16560 acgaagctat gtccaagcgc aaaatcaaag aagagatgct ccaggtcatc gcgccggaga 16620 tctatggccc cccgaagaag gaagagcagg attacaagcc ccgaaagcta aagcgggtca 16680 aaaagaaaaa gaaagatgat gatgatgaac ttgacgacga ggtggaactg ctgcacgcta 16740 ccgcgcccag gcgacgggta cagtggaaag gtcgacgcgt aaaacgtgtt ttgcgacccg 16800 gcaccaccgt agtctttacg cccggtgagc gctccacccg cacctacaag cgcgtgtatg 16860 atgaggtgta cggcgacgag gacctgcttg agcaggccaa cgagcgcctc ggggagtttg 16920 cctacggaaa gcggcataag gacatgctgg cgttgccgct ggacgagggc aacccaacac 16980 ctagcctaaa gcccgtaaca ctgcagcagg tgctgcccgc gcttgcaccg tccgaagaaa 17040 agcgcggcct aaagcgcgag tctggtgact tggcacccac cgtgcagctg atggtaccca 17100 agcgccagcg actggaagat gtcttggaaa aaatgaccgt ggaacctggg ctggagcccg 17160 aggtccgcgt gcggccaatc aagcaggtgg cgccgggact gggcgtgcag accgtggacg 17220 ttcagatacc cactaccagt agcaccagta ttgccaccgc cacagagggc atggagacac 17280 aaacgtcccc ggttgcctca gcggtggcgg atgccgcggt gcaggcggtc gctgcggccg 17340 cgtccaagac ctctacggag gtgcaaacgg acccgtggat gtttcgcgtt tcagcccccc 17400 ggcgcccgcg cggttcgagg aagtacggcg ccgccagcgc gctactgccc gaatatgccc 17460 tacatccttc cattgcgcct acccccggct atcgtggcta cacctaccgc cccagaagac 17520 gagcaactac ccgacgccga accaccactg gaacccgccg ccgccgtcgc cgtcgccagc 17580 ccgtgctggc cccgatttcc gtgcgcaggg tggctcgcga aggaggcagg accctggtgc 17640 tgccaacagc gcgctaccac cccagcatcg tttaaaagcc ggtctttgtg gttcttgcag 17700 atatggccct cacctgccgc ctccgtttcc cggtgccggg attccgagga agaatgcacc 17760 gtaggagggg catggccggc cacggcctga cgggcggcat gcgtcgtgcg caccaccggc 17820 ggcggcgcgc gtcgcaccgt cgcatgcgcg gcggtatcct gcccctcctt attccactga 17880 tcgccgcggc gattggcgcc gtgcccggaa ttgcatccgt ggccttgcag gcgcagagac 17940 actgattaaa aacaagttgc atgtggaaaa atcaaaataa aaagtctgga ctctcacgct 18000 cgcttggtcc tgtaactatt ttgtagaatg gaagacatca actttgcgtc tctggccccg 18060 cgacacggct cgcgcccgtt catgggaaac tggcaagata tcggcaccag caatatgagc 18120 ggtggcgcct tcagctgggg ctcgctgtgg agcggcatta aaaatttcgg ttccaccgtt 18180 aagaactatg gcagcaaggc ctggaacagc agcacaggcc agatgctgag ggataagttg 18240 aaagagcaaa atttccaaca aaaggtggta gatggcctgg cctctggcat tagcggggtg 18300 gtggacctgg ccaaccaggc agtgcaaaat aagattaaca gtaagcttga tccccgccct 18360 cccgtagagg agcctccacc ggccgtggag acagtgtctc cagaggggcg tggcgaaaag 18420 cgtccgcgcc ccgacaggga agaaactctg gtgacgcaaa tagacgagcc tccctcgtac 18480 gaggaggcac taaagcaagg cctgcccacc acccgtccca tcgcgcccat ggctaccgga 18540 gtgctgggcc agcacacacc cgtaacgctg gacctgcctc cccccgccga cacccagcag 18600 aaacctgtgc tgccaggccc gaccgccgtt gttgtaaccc gtcctagccg cgcgtccctg 18660 cgccgcgccg ccagcggtcc gcgatcgttg cggcccgtag ccagtggcaa ctggcaaagc 18720 acactgaaca gcatcgtggg tctgggggtg caatccctga agcgccgacg atgcttctga 18780 atagctaacg tgtcgtatgt gtgtcatgta tgcgtccatg tcgccgccag aggagctgct 18840 gagccgccgc gcgcccgctt tccaagatgg ctaccccttc gatgatgccg cagtggtctt 18900 acatgcacat ctcgggccag gacgcctcgg agtacctgag ccccgggctg gtgcagtttg 18960 cccgcgccac cgagacgtac ttcagcctga ataacaagtt tagaaacccc acggtggcgc 19020 ctacgcacga cgtgaccaca gaccggtccc agcgtttgac gctgcggttc atccctgtgg 19080 accgtgagga tactgcgtac tcgtacaagg cgcggttcac cctagctgtg ggtgataacc 19140 gtgtgctgga catggcttcc acgtactttg acatccgcgg cgtgctggac aggggcccta 19200 cttttaagcc ctactctggc actgcctaca acgccctggc tcccaagggt gccccaaatc 19260 cttgcgaatg ggatgaagct gctactgctc ttgaaataaa cctagaagaa gaggacgatg 19320 acaacgaaga cgaagtagac gagcaagctg agcagcaaaa aactcacgta tttgggcagg 19380 cgccttattc tggtataaat attacaaagg agggtattca aataggtgtc gaaggtcaaa 19440 cacctaaata tgccgataaa acatttcaac ctgaacctca aataggagaa tctcagtggt 19500 acgaaactga aattaatcat gcagctggga gagtccttaa aaagactacc ccaatgaaac 19560 catgttacgg ttcatatgca aaacccacaa atgaaaatgg agggcaaggc attcttgtaa 19620 agcaacaaaa tggaaagcta gaaagtcaag tggaaatgca atttttctca actactgagg 19680 cgaccgcagg caatggtgat aacttgactc ctaaagtggt attgtacagt gaagatgtag 19740 atatagaaac cccagacact catatttctt acatgcccac tattaaggaa ggtaactcac 19800 gagaactaat gggccaacaa tctatgccca acaggcctaa ttacattgct tttagggaca 19860 attttattgg tctaatgtat tacaacagca cgggtaatat gggtgttctg gcgggccaag 19920 catcgcagtt gaatgctgtt gtagatttgc aagacagaaa cacagagctt tcataccagc 19980 ttttgcttga ttccattggt gatagaacca ggtacttttc tatgtggaat caggctgttg 20040 acagctatga tccagatgtt agaattattg aaaatcatgg aactgaagat gaacttccaa 20100 attactgctt tccactggga ggtgtgatta atacagagac tcttaccaag gtaaaaccta 20160 aaacaggtca ggaaaatgga tgggaaaaag atgctacaga attttcagat aaaaatgaaa 20220 taagagttgg aaataatttt gccatggaaa tcaatctaaa tgccaacctg tggagaaatt 20280 tcctgtactc caacatagcg ctgtatttgc ccgacaagct aaagtacagt ccttccaacg 20340 taaaaatttc tgataaccca aacacctacg actacatgaa caagcgagtg gtggctcccg 20400 ggttagtgga ctgctacatt aaccttggag cacgctggtc ccttgactat atggacaacg 20460 tcaacccatt taaccaccac cgcaatgctg gcctgcgcta ccgctcaatg ttgctgggca 20520 atggtcgcta tgtgcccttc cacatccagg tgcctcagaa gttctttgcc attaaaaacc 20580 tccttctcct gccgggctca tacacctacg agtggaactt caggaaggat gttaacatgg 20640 ttctgcagag ctccctagga aatgacctaa gggttgacgg agccagcatt aagtttgata 20700 gcatttgcct ttacgccacc ttcttcccca tggcccacaa caccgcctcc acgcttgagg 20760 ccatgcttag aaacgacacc aacgaccagt cctttaacga ctatctctcc gccgccaaca 20820 tgctctaccc tatacccgcc aacgctacca acgtgcccat atccatcccc tcccgcaact 20880 gggcggcttt ccgcggctgg gccttcacgc gccttaagac taaggaaacc ccatcactgg 20940 gctcgggcta cgacccttat tacacctact ctggctctat accctaccta gatggaacct 21000 tttacctcaa ccacaccttt aagaaggtgg ccattacctt tgactcttct gtcagctggc 21060 ctggcaatga ccgcctgctt acccccaacg agtttgaaat taagcgctca gttgacgggg 21120 agggttacaa cgttgcccag tgtaacatga ccaaagactg gttcctggta caaatgctag 21180 ctaactacaa cattggctac cagggcttct atatcccaga gagctacaag gaccgcatgt 21240 actccttctt tagaaacttc cagcccatga gccgtcaggt ggtggatgat actaaataca 21300 aggactacca acaggtgggc atcctacacc aacacaacaa ctctggattt gttggctacc 21360 ttgcccccac catgcgcgaa ggacaggcct accctgctaa cttcccctat ccgcttatag 21420 gcaagaccgc agttgacagc attacccaga aaaagtttct ttgcgatcgc accctttggc 21480 gcatcccatt ctccagtaac tttatgtcca tgggcgcact cacagacctg ggccaaaacc 21540 ttctctacgc caactccgcc cacgcgctag acatgacttt tgaggtggat cccatggacg 21600 agcccaccct tctttatgtt ttgtttgaag tctttgacgt ggtccgtgtg caccggccgc 21660 accgcggcgt catcgaaacc gtgtacctgc gcacgccctt ctcggccggc aacgccacaa 21720 cataaagaag caagcaacat caacaacagc tgccgccatg ggctccagtg agcaggaact 21780 gaaagccatt gtcaaagatc ttggttgtgg gccatatttt ttgggcacct atgacaagcg 21840 ctttccaggc tttgtttctc cacacaagct cgcctgcgcc atagtcaata cggccggtcg 21900 cgagactggg ggcgtacact ggatggcctt tgcctggaac ccgcactcaa aaacatgcta 21960 cctctttgag ccctttggct tttctgacca gcgactcaag caggtttacc agtttgagta 22020 cgagtcactc ctgcgccgta gcgccattgc ttcttccccc gaccgctgta taacgctgga 22080 aaagtccacc caaagcgtac aggggcccaa ctcggccgcc tgtggactat tctgctgcat 22140 gtttctccac gcctttgcca actggcccca aactcccatg gatcacaacc ccaccatgaa 22200 ccttattacc ggggtaccca actccatgct caacagtccc caggtacagc ccaccctgcg 22260 tcgcaaccag gaacagctct acagcttcct ggagcgccac tcgccctact tccgcagcca 22320 cagtgcgcag attaggagcg ccacttcttt ttgtcacttg aaaaacatgt aaaaataatg 22380 tactagagac actttcaata aaggcaaatg cttttatttg tacactctcg ggtgattatt 22440 tacccccacc cttgccgtct gcgccgttta aaaatcaaag gggttctgcc gcgcatcgct 22500 atgcgccact ggcagggaca cgttgcgata ctggtgttta gtgctccact taaactcagg 22560 cacaaccatc cgcggcagct cggtgaagtt ttcactccac aggctgcgca ccatcaccaa 22620 cgcgtttagc aggtcgggcg ccgatatctt gaagtcgcag ttggggcctc cgccctgcgc 22680 gcgcgagttg cgatacacag ggttgcagca ctggaacact atcagcgccg ggtggtgcac 22740 gctggccagc acgctcttgt cggagatcag atccgcgtcc aggtcctccg cgttgctcag 22800 ggcgaacgga gtcaactttg gtagctgcct tcccaaaaag ggcgcgtgcc caggctttga 22860 gttgcactcg caccgtagtg gcatcaaaag gtgaccgtgc ccggtctggg cgttaggata 22920 cagcgcctgc ataaaagcct tgatctgctt aaaagccacc tgagcctttg cgccttcaga 22980 gaagaacatg ccgcaagact tgccggaaaa ctgattggcc ggacaggccg cgtcgtgcac 23040 gcagcacctt gcgtcggtgt tggagatctg caccacattt cggccccacc ggttcttcac 23100 gatcttggcc ttgctagact gctccttcag cgcgcgctgc ccgttttcgc tcgtcacatc 23160 catttcaatc acgtgctcct tatttatcat aatgcttccg tgtagacact taagctcgcc 23220 ttcgatctca gcgcagcggt gcagccacaa cgcgcagccc gtgggctcgt gatgcttgta 23280 ggtcacctct gcaaacgact gcaggtacgc ctgcaggaat cgccccatca tcgtcacaaa 23340 ggtcttgttg ctggtgaagg tcagctgcaa cccgcggtgc tcctcgttca gccaggtctt 23400 gcatacggcc gccagagctt ccacttggtc aggcagtagt ttgaagttcg cctttagatc 23460 gttatccacg tggtacttgt ccatcagcgc gcgcgcagcc tccatgccct tctcccacgc 23520 agacacgatc ggcacactca gcgggttcat caccgtaatt tcactttccg cttcgctggg 23580 ctcttcctct tcctcttgcg tccgcatacc acgcgccact gggtcgtctt cattcagccg 23640 ccgcactgtg cgcttacctc ctttgccatg cttgattagc accggtgggt tgctgaaacc 23700 caccatttgt agcgccacat cttctctttc ttcctcgctg tccacgatta cctctggtga 23760 tggcgggcgc tcgggcttgg gagaagggcg cttctttttc ttcttgggcg caatggccaa 23820 atccgccgcc gaggtcgatg gccgcgggct gggtgtgcgc ggcaccagcg cgtcttgtga 23880 tgagtcttcc tcgtcctcgg actcgatacg ccgcctcatc cgcttttttg ggggcgcccg 23940 gggaggcggc ggcgacgggg acggggacga cacgtcctcc atggttgggg gacgtcgcgc 24000 cgcaccgcgt ccgcgctcgg gggtggtttc gcgctgctcc tcttcccgac tggccatttc 24060 cttctcctat aggcagaaaa agatcatgga gtcagtcgag aagaaggaca gcctaaccgc 24120 cccctctgag ttcgccacca ccgcctccac cgatgccgcc aacgcgccta ccaccttccc 24180 cgtcgaggca cccccgcttg aggaggagga agtgattatc gagcaggacc caggttttgt 24240 aagcgaagac gacgaggacc gctcagtacc aacagaggat aaaaagcaag accaggacaa 24300 cgcagaggca aacgaggaac aagtcgggcg gggggacgaa aggcatggcg actacctaga 24360 tgtgggagac gacgtgctgt tgaagcatct gcagcgccag tgcgccatta tctgcgacgc 24420 gttgcaagag cgcagcgatg tgcccctcgc catagcggat gtcagccttg cctacgaacg 24480 ccacctattc tcaccgcgcg taccccccaa acgccaagaa aacggcacat gcgagcccaa 24540 cccgcgcctc aacttctacc ccgtatttgc cgtgccagag gtgcttgcca cctatcacat 24600 ctttttccaa aactgcaaga tacccctatc ctgccgtgcc aaccgcagcc gagcggacaa 24660 gcagctggcc ttgcggcagg gcgctgtcat acctgatatc gcctcgctca acgaagtgcc 24720 aaaaatcttt gagggtcttg gacgcgacga gaagcgcgcg gcaaacgctc tgcaacagga 24780 aaacagcgaa aatgaaagtc actctggagt gttggtggaa ctcgagggtg acaacgcgcg 24840 cctagccgta ctaaaacgca gcatcgaggt cacccacttt gcctacccgg cacttaacct 24900 accccccaag gtcatgagca cagtcatgag tgagctgatc gtgcgccgtg cgcagcccct 24960 ggagagggat gcaaatttgc aagaacaaac agaggagggc ctacccgcag ttggcgacga 25020 gcagctagcg cgctggcttc aaacgcgcga gcctgccgac ttggaggagc gacgcaaact 25080 aatgatggcc gcagtgctcg ttaccgtgga gcttgagtgc atgcagcggt tctttgctga 25140 cccggagatg cagcgcaagc tagaggaaac attgcactac acctttcgac agggctacgt 25200 acgccaggcc tgcaagatct ccaacgtgga gctctgcaac ctggtctcct accttggaat 25260 tttgcacgaa aaccgccttg ggcaaaacgt gcttcattcc acgctcaagg gcgaggcgcg 25320 ccgcgactac gtccgcgact gcgtttactt atttctatgc tacacctggc agacggccat 25380 gggcgtttgg cagcagtgct tggaggagtg caacctcaag gagctgcaga aactgctaaa 25440 gcaaaacttg aaggacctat ggacggcctt caacgagcgc tccgtggccg cgcacctggc 25500 ggacatcatt ttccccgaac gcctgcttaa aaccctgcaa cagggtctgc cagacttcac 25560 cagtcaaagc atgttgcaga actttaggaa ctttatccta gagcgctcag gaatcttgcc 25620 cgccacctgc tgtgcacttc ctagcgactt tgtgcccatt aagtaccgcg aatgccctcc 25680 gccgctttgg ggccactgct accttctgca gctagccaac taccttgcct accactctga 25740 cataatggaa gacgtgagcg gtgacggtct actggagtgt cactgtcgct gcaacctatg 25800 caccccgcac cgctccctgg tttgcaattc gcagctgctt aacgaaagtc aaattatcgg 25860 tacctttgag ctgcagggtc cctcgcctga cgaaaagtcc gcggctccgg ggttgaaact 25920 cactccgggg ctgtggacgt cggcttacct tcgcaaattt gtacctgagg actaccacgc 25980 ccacgagatt aggttctacg aagaccaatc ccgcccgcca aatgcggagc ttaccgcctg 26040 cgtcattacc cagggccaca ttcttggcca attgcaagcc atcaacaaag cccgccaaga 26100 gtttctgcta cgaaagggac ggggggttta cttggacccc cagtccggcg aggagctcaa 26160 cccaatcccc ccgccgccgc agccctatca gcagcagccg cgggcccttg cttcccagga 26220 tggcacccaa aaagaagctg cagctgccgc cgccacccac ggacgaggag gaatactggg 26280 acagtcaggc agaggaggtt ttggacgagg aggaggagga catgatggaa gactgggaga 26340 gcctagacga ggaagcttcc gaggtcgaag aggtgtcaga cgaaacaccg tcaccctcgg 26400 tcgcattccc ctcgccggcg ccccagaaat cggcaaccgg ttccagcatg gctacaacct 26460 ccgctcctca ggcgccgccg gcactgcccg ttcgccgacc caaccgtaga tgggacacca 26520 ctggaaccag ggccggtaag tccaagcagc cgccgccgtt agcccaagag caacaacagc 26580 gccaaggcta ccgctcatgg cgcgggcaca agaacgccat agttgcttgc ttgcaagact 26640 gtgggggcaa catctccttc gcccgccgct ttcttctcta ccatcacggc gtggccttcc 26700 cccgtaacat cctgcattac taccgtcatc tctacagccc atactgcacc ggcggcagcg 26760 gcagcggcag caacagcagc ggccacacag aagcaaaggc gaccggatag caagactctg 26820 acaaagccca agaaatccac agcggcggca gcagcaggag gaggagcgct gcgtctggcg 26880 cccaacgaac ccgtatcgac ccgcgagctt agaaacagga tttttcccac tctgtatgct 26940 atatttcaac agagcagggg ccaagaacaa gagctgaaaa taaaaaacag gtctctgcga 27000 tccctcaccc gcagctgcct gtatcacaaa agcgaagatc agcttcggcg cacgctggaa 27060 gacgcggagg ctctcttcag taaatactgc gcgctgactc ttaaggacta gtttcgcgcc 27120 ctttctcaaa tttaagcgcg aaaactacgt catctccagc ggccacaccc ggcgccagca 27180 cctgtcgtca gcgccatttc aactttgtat acaaaagttg tgatgagcaa ggaaattccc 27240 acgccctaca tgtggagtta ccagccacaa atgggacttg cggctggagc tgcccaagac 27300 tactcaaccc gaataaacta catgagcgcg ggaccccaca tgatatcccg ggtcaacgga 27360 atacgcgccc accgaaaccg aattctcctg gaacaggcgg ctattaccac cacacctcgt 27420 aataacctta atccccgtag ttggcccgct gccctggtgt accaggaaag tcccgctccc 27480 accactgtgg tacttcccag agacgcccag gccgaagttc agatgactaa ctcaggggcg 27540 cagcttgcgg gcggctttcg tcacagggtg cggtcgcccg ggcagggtat aactcacctg 27600 acaatcagag ggcgaggtat tcagctcaac gacgagtcgg tgagctcctc gcttggtctc 27660 cgtccggacg ggacatttca gatcggcggc gccggccgct cttcattcac gcctcgtcag 27720 gcaatcctaa ctctgcagac ctcgtcctct gagccgcgct ctggaggcat tggaactctg 27780 caatttattg aggagtttgt gccatcggtc tactttaacc ccttctcggg acctcccggc 27840 cactatccgg atcaatttat tcctaacttt gacgcggtaa aggactcggc ggacggctac 27900 gactgaatgt taagtggaga ggcagagcaa ctgcgcctga aacacctggt ccactgtcgc 27960 cgccacaagt gctttgcccg cgactccggt gagttttgct actttgaatt gcccgaggat 28020 catatcgagg gcccggcgca cggcgtccgg cttaccgccc agggagagct tgcccgtagc 28080 ctgattcggg agtttaccca gcgccccctg ctagttgagc gggacagggg accctgtgtt 28140 ctcactgtga tttgcaactg tcctaaccct ggattacatc aagatctttg ttgccatctc 28200 tgtgctgagt ataataaata cagaaattaa aatatactgg ggctcctatc gccatcctgt 28260 aaacgccacc gtcttcaccc gcccaagcaa accaaggcga accttacctg gtacttttaa 28320 catctctccc tctgtgattt acaacagttt caacccagac ggagtgagtc tacgagagaa 28380 cctctccgag ctcagctact ccatcagaaa aaacaccacc ctccttacct gccgggaacg 28440 tacgagtgcg tcaccggccg ctgcaccaca cctaccgcct gaccgtaaac cagacttttt 28500 ccggacagac ctcaataact ctgtttacca gaacaggagg tgagcttaga aaacccttag 28560 ggtattaggc caaaggcgca gctactgtgg ggtttatgaa caattcaagc aactctacgg 28620 gctattctaa ttcaggtttc tctagaatcg gggttggggt tattctctgt cttgtgattc 28680 tctttattct tatactaacg cttctctgcc taaggctcgc cgcctgctgt gtgcacattt 28740 gcatttattg tcagcttttt aaacgctggg gtcgccaccc aagatgatta ggtacataat 28800 cctaggttta ctcacccttg cgtcagccca cggtaccacc caaaaggtgg attttaagga 28860 gccagcctgt aatgttacat tcgcagctga agctaatgag tgcaccactc ttataaaatg 28920 caccacagaa catgaaaagc tgcttattcg ccacaaaaac aaaattggca agtatgctgt 28980 ttatgctatt tggcagccag gtgacactac agagtataat gttacagttt tccagggtaa 29040 aagtcataaa acttttatgt atacttttcc attttatgaa atgtgcgaca ttaccatgta 29100 catgagcaaa cagtataagt tgtggccccc acaaaattgt gtggaaaaca ctggcacttt 29160 ctgctgcact gctatgctaa ttacagtgct cgctttggtc tgtaccctac tctatattaa 29220 atacaaaagc agacgcagct ttattgagga aaagaaaatg ccttaattta ctaagttaca 29280 aagctaatgt caccactaac tgctttactc gctgcttgca aaacaaattc aaaaagttag 29340 cattataatt agaataggat ttaaaccccc cggtcatttc ctgctcaata ccattcccct 29400 gaacaattga ctctatgtgg gatatgctcc agcgctacaa ccttgaagtc aggcttcctg 29460 gatgtcagca tctgactttg gccagcacct gtcccgcgga tttgttccag tccaactaca 29520 gcgacccacc ctaacagaga tgaccaacac aaccaacgcg gccgccgcta ccggacttac 29580 atctaccaca aatacacccc aagtttctgc ctttgtcaat aactgggata acttgggcat 29640 gtggtggttc tccatagcgc ttatgtttgt atgccttatt attatgtggc tcatctgctg 29700 cctaaagcgc aaacgcgccc gaccacccat ctatagtccc atcattgtgc tacacccaaa 29760 caatgatgga atccatagat tggacggact gaaacacatg ttcttttctc ttacagtagg 29820 aagcggagct actaacttca gcctgctgaa gcaggctggt gacgtcgagg agaatcctgg 29880 cccaatggtg agcaaaggcg aagagctgtt caccggcgtg gtgcccatcc tggtggagct 29940 ggacggcgac gtgaacggcc acaagttcag cgtgagcggc gagggcgagg gcgacgccac 30000 ctacggcaag ctgaccctga agctgctgtg caccaccggc aagctgcccg tgccctggcc 30060 caccctggtg accaccctgg gctacggcgt gcagtgcttc gcccggtacc ccgaccacat 30120 gaagcagcac gacttcttca agagcgccat gcccgagggc tacgtgcagg agcggaccat 30180 cttcttcaag gacgacggca actacaagac ccgggccgag gtgaagttcg agggcgacac 30240 cctggtgaac cggatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg 30300 ccacaagctg gagtacaact acaacagcca caacgtgtac atcaccgccg acaagcagaa 30360 gaacggcatc aaggccaact tcaagatccg gcacaacatc gaggacggcg gcgtgcagct 30420 ggccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa 30480 ccactacctg agctaccaga gcgccctgtt caaggacccc aacgagaagc gggaccacat 30540 ggtgctgctg gagttcctga ccgccgccgg catcaccgag ggcatgaacg agctctataa 30600 gtgattaaat gagacatgat tcctcgagtt tttatattac tgacccttgt tgcgcttttt 30660 tgtgcgtgct ccacattggc tgcggtttct cacatcgaag tagactgcat tccagccttc 30720 acagtctatt tgctttacgg atttgtcacc ctcacgctca tctgcagcct catcactgtg 30780 gtcatcgcct ttatccagtg cattgactgg gtctgtgtgc gctttgcata tctcagacac 30840 catccccagt acagggacag gactatagct gagcttctta gaattcttta attatgaaat 30900 ttactgtgac ttttctgctg attatttgca ccctatctgc gttttgttcc ccgacctcca 30960 agcctcaaag acatatatca tgcagattca ctcgtatatg gaatattcca agttgctaca 31020 atgaaaaaag cgatctttcc gaagcctggt tatatgcaat catctctgtt atggtgttct 31080 gcagtaccat cttagcccta gctatatatc cctaccttga cattggctgg aacgcaatag 31140 atgccatgaa ccacccaact ttccccgcgc ccgctatgct tccactgcaa caagttgttg 31200 ccggcggctt tgtcccagcc aatcagcctc gcccaccttc tcccaccccc actgaaatca 31260 gctactttaa tctaacagga ggagatgact gacaccctag atctagaaat ggacggaatt 31320 attacagagc agcgcctgct agaaagacgc agggcagcgg ccgagcaaca gcgcatgaat 31380 caagagctcc aagacatggt taacttgcac cagtgcaaaa ggggtatctt ttgtctggta 31440 aagcaggcca aagtcaccta cgacagtaat accaccggac accgccttag ctacaagttg 31500 ccaaccaagc gtcagaaatt ggtggtcatg gtgggagaaa agcccattac cataactcag 31560 cactcggtag aaaccgaagg ctgcattcac tcaccttgtc aaggacctga ggatctctgc 31620 acccttatta agaccctgtg cggtctcaaa gatcttattc cctttaacta ataaaaaaaa 31680 ataataaagc atcacttact taaaatcagt tagcaaattt ctgtccagtt tattcagcag 31740 cacctccttg ccctcctccc agctctggta ttgcagcttc ctcctggctg caaactttct 31800 ccacaatcta aatggaatgt cagtttcctc ctgttcctgt ccatccgcac ccactatctt 31860 catgttgttg cagatgaagc gcgcaagacc gtctgaagat accttcaacc ccgtgtatcc 31920 atatgacacg gaaaccggtc ctccaactgt gccttttctt actcctccct ttgtatcccc 31980 caatgggttt caagagagtc cccctggggt actctctttg cgcctatccg aacctctagt 32040 tacctccaat ggcatgcttg cgctcaaaat gggcaacggc ctctctctgg acgaggccgg 32100 caaccttacc tcccaaaatg taaccactgt gagcccacct ctcaaaaaaa ccaagtcaaa 32160 cataaacctg gaaatatctg cacccctcac agttacctca gaagccctaa ctgtggctgc 32220 cgccgcacct ctaatggtcg cgggcaacac actcaccatg caatcacagg ccccgctaac 32280 cgtgcacgac tccaaactta gcattgccac ccaaggaccc ctcacagtgt cagaaggaaa 32340 gctagccctg caaacatcag gccccctcac caccaccgat agcagtaccc ttactatcac 32400 tgcctcaccc cctctaacta ctgccactgg tagcttgggc attgacttga aagagcccat 32460 ttatacacaa aatggaaaac taggactaaa gtacggggct cctttgcatg taacagacga 32520 cctaaacact ttgaccgtag caactggtcc aggtgtgact attaataata cttccttgca 32580 aactaaagtt actggagcct tgggttttga ttcacaaggc aatatgcaac ttaatgtagc 32640 aggaggacta aggattgatt ctcaaaacag acgccttata cttgatgtta gttatccgtt 32700 tgatgctcaa aaccaactaa atctaagact aggacagggc cctcttttta taaactcagc 32760 ccacaacttg gatattaact acaacaaagg cctttacttg tttacagctt caaacaattc 32820 caaaaagctt gaggttaacc taagcactgc caaggggttg atgtttgacg ctacagccat 32880 agccattaat gcaggagatg ggcttgaatt tggttcacct aatgcaccaa acacaaatcc 32940 cctcaaaaca aaaattggcc atggcctaga atttgattca aacaaggcta tggttcctaa 33000 actaggaact ggccttagtt ttgacagcac aggtgccatt acagtaggaa acaaaaataa 33060 tgataagcta actttgtgga ccacaccagc tccatctcct aactgtagac taaatgcaga 33120 gaaagatgct aaactcactt tggtcttaac aaaatgtggc agtcaaatac ttgctacagt 33180 ttcagttttg gctgttaaag gcagtttggc tccaatatct ggaacagttc aaagtgctca 33240 tcttattata agatttgacg aaaatggagt gctactaaac aattccttcc tggacccaga 33300 atattggaac tttagaaatg gagatcttac tgaaggcaca gcctatacaa acgctgttgg 33360 atttatgcct aacctatcag cttatccaaa atctcacggt aaaactgcca aaagtaacat 33420 tgtcagtcaa gtttacttaa acggagacaa aactaaacct gtaacactaa ccattacact 33480 aaacggtaca caggaaacag gagacacaac tccaagtgca tactctatgt cattttcatg 33540 ggactggtct ggccacaact acattaatga aatatttgcc acatcctctt acactttttc 33600 atacattgcc caagaataaa gaatcgtttg tgttatgttt caacgtgttt atttttcaat 33660 tgcagaaaat ttcgaatcat ttttcattca gtagtatagc cccaccacca catagcttat 33720 acagatcacc gtacctcaac tttgtataat aaagttgtaa tcaaactcac agaaccctag 33780 tattcaacct gccacctccc tcccaacaca cagagtacac agtcctttct ccccggctgg 33840 ccttaaaaag catcatatca tgggtaacag acatattctt aggtgttata ttccacacgg 33900 tttcctgtcg agccaaacgc tcatcagtga tattaataaa ctccccgggc agctcactta 33960 agttcatgtc gctgtccagc tgctgagcca caggctgctg tccaacttgc ggttgcttaa 34020 cgggcggcga aggagaagtc cacgcctaca tgggggtaga gtcataatcg tgcatcagga 34080 tagggcggtg gtgctgcagc agcgcgcgaa taaactgctg ccgccgccgc tccgtcctgc 34140 aggaatacaa catggcagtg gtctcctcag cgatgattcg caccgcccgc agcataaggc 34200 gccttgtcct ccgggcacag cagcgcaccc tgatctcact taaatcagca cagtaactgc 34260 agcacagcac cacaatattg ttcaaaatcc cacagtgcaa ggcgctgtat ccaaagctca 34320 tggcggggac cacagaaccc acgtggccat cataccacaa gcgcaggtag attaagtggc 34380 gacccctcat aaacacgctg gacataaaca ttacctcttt tggcatgttg taattcacca 34440 cctcccggta ccatataaac ctctgattaa acatggcgcc atccaccacc atcctaaacc 34500 agctggccaa aacctgcccg ccggctatac actgcaggga accgggactg gaacaatgac 34560 agtggagagc ccaggactcg taaccatgga tcatcatgct cgtcatgata tcaatgttgg 34620 cacaacacag gcacacgtgc atacacttcc tcaggattac aagctcctcc cgcgttagaa 34680 ccatatccca gggaacaacc cattcctgaa tcagcgtaaa tcccacactg cagggaagac 34740 ctcgcacgta actcacgttg tgcattgtca aagtgttaca ttcgggcagc agcggatgat 34800 cctccagtat ggtagcgcgg gtttctgtct caaaaggagg tagacgatcc ctactgtacg 34860 gagtgcgccg agacaaccga gatcgtgttg gtcgtagtgt catgccaaat ggaacgccgg 34920 acgtagtcat atttcctgaa gcaaaaccag gtgcgggcgt gacaaacaga tctgcgtctc 34980 cggtctcgcc gcttagatcg ctctgtgtag tagttgtagt atatccactc tctcaaagca 35040 tccaggcgcc ccctggcttc gggttctatg taaactcctt catgcgccgc tgccctgata 35100 acatccacca ccgcagaata agccacaccc agccaaccta cacattcgtt ctgcgagtca 35160 cacacgggag gagcgggaag agctggaaga accatgtttt tttttttatt ccaaaagatt 35220 atccaaaacc tcaaaatgaa gatctattaa gtgaacgcgc tcccctccgg tggcgtggtc 35280 aaactctaca gccaaagaac agataatggc atttgtaaga tgttgcacaa tggcttccaa 35340 aaggcaaacg gccctcacgt ccaagtggac gtaaaggcta aacccttcag ggtgaatctc 35400 ctctataaac attccagcac cttcaaccat gcccaaataa ttctcatctc gccaccttct 35460 caatatatct ctaagcaaat cccgaatatt aagtccggcc attgtaaaaa tctgctccag 35520 agcgccctcc accttcagcc tcaagcagcg aatcatgatt gcaaaaattc aggttcctca 35580 cagacctgta taagattcaa aagcggaaca ttaacaaaaa taccgcgatc ccgtaggtcc 35640 cttcgcaggg ccagctgaac ataatcgtgc aggtctgcac ggaccagcgc ggccacttcc 35700 ccgccaggaa ccatgacaaa agaacccaca ctgattatga cacgcatact cggagctatg 35760 ctaaccagcg tagccccgat gtaagcttgt tgcatgggcg gcgatataaa atgcaaggtg 35820 ctgctcaaaa aatcaggcaa agcctcgcgc aaaaaagaaa gcacatcgta gtcatgctca 35880 tgcagataaa ggcaggtaag ctccggaacc accacagaaa aagacaccat ttttctctca 35940 aacatgtctg cgggtttctg cataaacaca aaataaaata acaaaaaaac atttaaacat 36000 tagaagcctg tcttacaaca ggaaaaacaa cccttataag cataagacgg actacggcca 36060 tgccggcgtg accgtaaaaa aactggtcac cgtgattaaa aagcaccacc gacagctcct 36120 cggtcatgtc cggagtcata atgtaagact cggtaaacac atcaggttga ttcacatcgg 36180 tcagtgctaa aaagcgaccg aaatagcccg ggggaataca tacccgcagg cgtagagaca 36240 acattacagc ccccatagga ggtataacaa aattaatagg agagaaaaac acataaacac 36300 ctgaaaaacc ctcctgccta ggcaaaatag caccctcccg ctccagaaca acatacagcg 36360 cttccacagc ggcagccata acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa 36420 aacaccactc gacacggcac cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag 36480 agcgagtata tataggacta aaaaatgacg taacggttaa agtccacaaa aaacacccag 36540 aaaaccgcac gcgaacctac gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa 36600 tcgtcacttc cgttttccca cgttacgtca cttcccattt taagaaaact acaattccca 36660 acacatacaa gttactccgc cctaaaacct acgtcacccg ccccgttccc acgccccgcg 36720 ccacgtcaca aactccaccc cctcattatc atattggctt caatccaaaa taaggtatat 36780 tattgatgat g 36791 <210> 9 <211> 36720 <212> DNA <213> Artificial Sequence <220> <223> Synthetic adenovirus genome E3-14.7k-P2A-YPet <400> 9 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420 cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480 tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540 tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600 aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660 tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720 cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780 gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840 cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900 ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960 cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020 caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080 ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140 tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200 gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260 ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320 cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380 ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440 gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500 cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560 ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620 gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680 cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740 ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800 tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860 gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920 caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980 gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040 agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100 aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160 cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220 gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280 gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340 gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400 gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460 tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520 ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580 tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640 agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700 tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760 gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820 acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880 gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940 aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000 ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060 gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120 tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180 acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240 aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300 tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360 gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420 tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480 ttggctctag cgatgaagat acagattgag gtactgaaat gtgtgggcgt ggcttaaggg 3540 tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg 3600 ccgccgccat gagcaccaac tcgtttgatg gaagcattgt gagctcatat ttgacaacgc 3660 gcatgccccc atgggccggg gtgcgtcaga atgtgatggg ctccagcatt gatggtcgcc 3720 ccgtcctgcc cgcaaactct actaccttga cctacgagac cgtgtctgga acgccgttgg 3780 agactgcagc ctccgccgcc gcttcagccg ctgcagccac cgcccgcggg attgtgactg 3840 actttgcttt cctgagcccg cttgcaagca gtgcagcttc ccgttcatcc gcccgcgatg 3900 acaagttgac ggctcttttg gcacaattgg attctttgac ccgggaactt aatgtcgttt 3960 ctcagcagct gttggatctg cgccagcagg tttctgccct gaaggcttcc tcccctccca 4020 atgcggttta aaacataaat aaaaaaccag actctgtttg gatttggatc aagcataagt 4080 gtcttgctgt ctttatttag gggttttgcg cgcgcggtag gcccgggacc agcggtctcg 4140 gtcgttgagg gtcctgtgta ttttttccag gacgtggtaa aggtgactct ggatgttcag 4200 atacatgggc ataagcccgt ctctggggtg gaggtagcac cactgcagag cttcatgctg 4260 cggggtggtg ttgtagatga tccagtcgta gcaggagcgc tgggcgtggt gcctaaaaat 4320 gtctttcagt agcaagctga ttgccagggg caggcccttg gtgtaagtgt ttacaaagcg 4380 gttaagctgg gatgggtgca tacgtgggga tatgagatgc atcttggact gtatttttag 4440 gttggctatg ttcccagcca tatccctccg gggattcatg ttgtgcagaa ccaccagcac 4500 agtgtatccg gtgcacttgg gaaatttgtc atgtagctta gaaggaaatg cgtggaagaa 4560 cttggagacg cccttgtgac ctccaagatt ttccatgcat tcgtccataa tgatggcaat 4620 gggcccacgg gcggcggcct gggcgaagat atttctggga tcactaacgt catagttgtg 4680 ttccaggatg agatcgtcat aggccatttt tacaaagcgc gggcggaggg tgccagactg 4740 cggtataatg gttccatccg gcccaggggc gtagttaccc tcacagattt gcatttccca 4800 cgctttgagt tcagatgggg ggatcatgtc tacctgcggg gcgatgaaga aaacggtttc 4860 cggggtaggg gagatcagct gggaagaaag caggttcctg agcagctgcg acttaccgca 4920 gccggtgggc ccgtaaatca cacctattac cggctgcaac tggtagttaa gagagctgca 4980 gctgccgtca tccctgagca ggggggccac ttcgttaagc atgtccctga ctcgcatgtt 5040 ttccctgacc aaatccgcca gaaggcgctc gccgcccagc gatagcagtt cttgcaagga 5100 agcaaagttt ttcaacggtt tgagaccgtc cgccgtaggc atgcttttga gcgtttgacc 5160 aagcagttcc aggcggtccc acagctcggt cacctgctct acggcatctc gatccagcat 5220 atctcctcgt ttcgcgggtt ggggcggctt tcgctgtacg gcagtagtcg gtgctcgtcc 5280 agacgggcca gggtcatgtc tttccacggg cgcagggtcc tcgtcagcgt agtctgggtc 5340 acggtgaagg ggtgcgctcc gggctgcgcg ctggccaggg tgcgcttgag gctggtcctg 5400 ctggtgctga agcgctgccg gtcttcgccc tgcgcgtcgg ccaggtagca tttgaccatg 5460 gtgtcatagt ccagcccctc cgcggcgtgg cccttggcgc gcagcttgcc cttggaggag 5520 gcgccgcacg aggggcagtg cagacttttg agggcgtaga gcttgggcgc gagaaatacc 5580 gattccgggg agtaggcatc cgcgccgcag gccccgcaga cggtctcgca ttccacgagc 5640 caggtgagct ctggccgttc ggggtcaaaa accaggtttc ccccatgctt tttgatgcgt 5700 ttcttacctc tggtttccat gagccggtgt ccacgctcgg tgacgaaaag gctgtccgtg 5760 tccccgtata cagacttgag aggcctgtcc tcgagcggtg ttccgcggtc ctcctcgtat 5820 agaaactcgg accactctga gacaaaggct cgcgtccagg ccagcacgaa ggaggctaag 5880 tgggaggggt agcggtcgtt gtccactagg gggtccactc gctccagggt gtgaagacac 5940 atgtcgccct cttcggcatc aaggaaggtg attggtttgt aggtgtaggc cacgtgaccg 6000 ggtgttcctg aaggggggct ataaaagggg gtgggggcgc gttcgtcctc actctcttcc 6060 gcatcgctgt ctgcgagggc cagctgttgg ggtgagtact ccctctgaaa agcgggcatg 6120 acttctgcgc taagattgtc agtttccaaa aacgaggagg atttgatatt cacctggccc 6180 gcggtgatgc ctttgagggt ggccgcatcc atctggtcag aaaagacaat ctttttgttg 6240 tcaagcttgg tggcaaacga cccgtagagg gcgttggaca gcaacttggc gatggagcgc 6300 agggtttggt ttttgtcgcg atcggcgcgc tccttggccg cgatgtttag ctgcacgtat 6360 tcgcgcgcaa cgcaccgcca ttcgggaaag acggtggtgc gctcgtcggg caccaggtgc 6420 acgcgccaac cgcggttgtg cagggtgaca aggtcaacgc tggtggctac ctctccgcgt 6480 aggcgctcgt tggtccagca gaggcggccg cccttgcgcg agcagaatgg cggtaggggg 6540 tctagctgcg tctcgtccgg ggggtctgcg tccacggtaa agaccccggg cagcaggcgc 6600 gcgtcgaagt agtctatctt gcatccttgc aagtctagcg cctgctgcca tgcgcgggcg 6660 gcaagcgcgc gctcgtatgg gttgagtggg ggaccccatg gcatggggtg ggtgagcgcg 6720 gaggcgtaca tgccgcaaat gtcgtaaacg tagaggggct ctctgagtat tccaagatat 6780 gtagggtagc atcttccacc gcggatgctg gcgcgcacgt aatcgtatag ttcgtgcgag 6840 ggagcgagga ggtcgggacc gaggttgcta cgggcgggct gctctgctcg gaagactatc 6900 tgcctgaaga tggcatgtga gttggatgat atggttggac gctggaagac gttgaagctg 6960 gcgtctgtga gacctaccgc gtcacgcacg aaggaggcgt aggagtcgcg cagcttgttg 7020 accagctcgg cggtgacctg cacgtctagg gcgcagtagt ccagggtttc cttgatgatg 7080 tcatacttat cctgtccctt ttttttccac agctcgcggt tgaggacaaa ctcttcgcgg 7140 tctttccagt actcttggat cggaaacccg tcggcctccg aacggtaaga gcctagcatg 7200 tagaactggt tgacggcctg gtaggcgcag catccctttt ctacgggtag cgcgtatgcc 7260 tgcgcggcct tccggagcga ggtgtgggtg agcgcaaagg tgtccctgac catgactttg 7320 aggtactggt atttgaagtc agtgtcgtcg catccgccct gctcccagag caaaaagtcc 7380 gtgcgctttt tggaacgcgg atttggcagg gcgaaggtga catcgttgaa gagtatcttt 7440 cccgcgcgag gcataaagtt gcgtgtgatg cggaagggtc ccggcacctc ggaacggttg 7500 ttaattacct gggcggcgag cacgatctcg tcaaagccgt tgatgttgtg gcccacaatg 7560 taaagttcca agaagcgcgg gatgcccttg atggaaggca attttttaag ttcctcgtag 7620 gtgagctctt caggggagct gagcccgtgc tctgaaaggg cccagtctgc aagatgaggg 7680 ttggaagcga cgaatgagct ccacaggtca cgggccatta gcatttgcag gtggtcgcga 7740 aaggtcctaa actggcgacc tatggccatt ttttctgggg tgatgcagta gaaggtaagc 7800 gggtcttgtt cccagcggtc ccatccaagg ttcgcggcta ggtctcgcgc ggcagtcact 7860 agaggctcat ctccgccgaa cttcatgacc agcatgaagg gcacgagctg cttcccaaag 7920 gcccccatcc aagtataggt ctctacatcg taggtgacaa agagacgctc ggtgcgagga 7980 tgcgagccga tcgggaagaa ctggatctcc cgccaccaat tggaggagtg gctattgatg 8040 tggtgaaagt agaagtccct gcgacgggcc gaacactcgt gctggctttt gtaaaaacgt 8100 gcgcagtact ggcagcggtg cacgggctgt acatcctgca cgaggttgac ctgacgaccg 8160 cgcacaagga agcagagtgg gaatttgagc ccctcgcctg gcgggtttgg ctggtggtct 8220 tctacttcgg ctgcttgtcc ttgaccgtct ggctgctcga ggggagttac ggtggatcgg 8280 accaccacgc cgcgcgagcc caaagtccag atgtccgcgc gcggcggtcg gagcttgatg 8340 acaacatcgc gcagatggga gctgtccatg gtctggagct cccgcggcgt caggtcaggc 8400 gggagctcct gcaggtttac ctcgcataga cgggtcaggg cgcgggctag atccaggtga 8460 tacctaattt ccaggggctg gttggtggcg gcgtcgatgg cttgcaagag gccgcatccc 8520 cgcggcgcga ctacggtacc gcgcggcggg cggtgggccg cgggggtgtc cttggatgat 8580 gcatctaaaa gcggtgacgc gggcgagccc ccggaggtag ggggggctcc ggacccgccg 8640 ggagaggggg caggggcacg tcggcgccgc gcgcgggcag gagctggtgc tgcgcgcgta 8700 ggttgctggc gaacgcgacg acgcggcggt tgatctcctg aatctggcgc ctctgcgtga 8760 agacgacggg cccggtgagc ttgaacctga aagagagttc gacagaatca atttcggtgt 8820 cgttgacggc ggcctggcgc aaaatctcct gcacgtctcc tgagttgtct tgataggcga 8880 tctcggccat gaactgctcg atctcttcct cctggagatc tccgcgtccg gctcgctcca 8940 cggtggcggc gaggtcgttg gaaatgcggg ccatgagctg cgagaaggcg ttgaggcctc 9000 cctcgttcca gacgcggctg tagaccacgc ccccttcggc atcgcgggcg cgcatgacca 9060 cctgcgcgag attgagctcc acgtgccggg cgaagacggc gtagtttcgc aggcgctgaa 9120 agaggtagtt gagggtggtg gcggtgtgtt ctgccacgaa gaagtacata acccagcgtc 9180 gcaacgtgga ttcgttgata tcccccaagg cctcaaggcg ctccatggcc tcgtagaagt 9240 ccacggcgaa gttgaaaaac tgggagttgc gcgccgacac ggttaactcc tcctccagaa 9300 gacggatgag ctcggcgaca gtgtcgcgca cctcgcgctc aaaggctaca ggggcctctt 9360 cttcttcttc aatctcctct tccataaggg cctccccttc ttcttcttct ggcggcggtg 9420 ggggaggggg gacacggcgg cgacgacggc gcaccgggag gcggtcgaca aagcgctcga 9480 tcatctcccc gcggcgacgg cgcatggtct cggtgacggc gcggccgttc tcgcgggggc 9540 gcagttggaa gacgccgccc gtcatgtccc ggttatgggt tggcgggggg ctgccatgcg 9600 gcagggatac ggcgctaacg atgcatctca acaattgttg tgtaggtact ccgccgccga 9660 gggacctgag cgagtccgca tcgaccggat cggaaaacct ctcgagaaag gcgtctaacc 9720 agtcacagtc gcaaggtagg ctgagcaccg tggcgggcgg cagcgggcgg cggtcggggt 9780 tgtttctggc ggaggtgctg ctgatgatgt aattaaagta ggcggtcttg agacggcgga 9840 tggtcgacag aagcaccatg tccttgggtc cggcctgctg aatgcgcagg cggtcggcca 9900 tgccccaggc ttcgttttga catcggcgca ggtctttgta gtagtcttgc atgagccttt 9960 ctaccggcac ttcttcttct ccttcctctt gtcctgcatc tcttgcatct atcgctgcgg 10020 cggcggcgga gtttggccgt aggtggcgcc ctcttcctcc catgcgtgtg accccgaagc 10080 ccctcatcgg ctgaagcagg gctaggtcgg cgacaacgcg ctcggctaat atggcctgct 10140 gcacctgcgt gagggtagac tggaagtcat ccatgtccac aaagcggtgg tatgcgcccg 10200 tgttgatggt gtaagtgcag ttggccataa cggaccagtt aacggtctgg tgacccggct 10260 gcgagagctc ggtgtacctg agacgcgagt aagccctcga gtcaaatacg tagtcgttgc 10320 aagtccgcac caggtactgg tatcccacca aaaagtgcgg cggcggctgg cggtagaggg 10380 gccagcgtag ggtggccggg gctccggggg cgagatcttc caacataagg cgatgatatc 10440 cgtagatgta cctggacatc caggtgatgc cggcggcggt ggtggaggcg cgcggaaagt 10500 cgcggacgcg gttccagatg ttgcgcagcg gcaaaaagtg ctccatggtc gggacgctct 10560 ggccggtcag gcgcgcgcaa tcgttgacgc tctagaccgt gcaaaaggag agcctgtaag 10620 cgggcactct tccgtggtct ggtggataaa ttcgcaaggg tatcatggcg gacgaccggg 10680 gttcgagccc cgtatccggc cgtccgccgt gatccatgcg gttaccgccc gcgtgtcgaa 10740 cccaggtgtg cgacgtcaga caacggggga gtgctccttt tggcttcctt ccaggcgcgg 10800 cggctgctgc gctagctttt ttggccactg gccgcgcgca gcgtaagcgg ttaggctgga 10860 aagcgaaagc attaagtggc tcgctccctg tagccggagg gttattttcc aagggttgag 10920 tcgcgggacc cccggttcga gtctcggacc ggccggactg cggcgaacgg gggtttgcct 10980 ccccgtcatg caagaccccg cttgcaaatt cctccggaaa cagggacgag cccctttttt 11040 gcttttccca gatgcatccg gtgctgcggc agatgcgccc ccctcctcag cagcggcaag 11100 agcaagagca gcggcagaca tgcagggcac cctcccctcc tcctaccgcg tcaggagggg 11160 cgacatccgc ggttgacgcg gcagcagatg gtgattacga acccccgcgg cgccgggccc 11220 ggcactacct ggacttggag gagggcgagg gcctggcgcg gctaggagcg ccctctcctg 11280 agcggcaccc aagggtgcag ctgaagcgtg atacgcgtga ggcgtacgtg ccgcggcaga 11340 acctgtttcg cgaccgcgag ggagaggagc ccgaggagat gcgggatcga aagttccacg 11400 cagggcgcga gctgcggcat ggcctgaatc gcgagcggtt gctgcgcgag gaggactttg 11460 agcccgacgc gcgaaccggg attagtcccg cgcgcgcaca cgtggcggcc gccgacctgg 11520 taaccgcata cgagcagacg gtgaaccagg agattaactt tcaaaaaagc tttaacaacc 11580 acgtgcgtac gcttgtggcg cgcgaggagg tggctatagg actgatgcat ctgtgggact 11640 ttgtaagcgc gctggagcaa aacccaaata gcaagccgct catggcgcag ctgttcctta 11700 tagtgcagca cagcagggac aacgaggcat tcagggatgc gctgctaaac atagtagagc 11760 ccgagggccg ctggctgctc gatttgataa acatcctgca gagcatagtg gtgcaggagc 11820 gcagcttgag cctggctgac aaggtggccg ccatcaacta ttccatgctt agcctgggca 11880 agttttacgc ccgcaagata taccataccc cttacgttcc catagacaag gaggtaaaga 11940 tcgaggggtt ctacatgcgc atggcgctga aggtgcttac cttgagcgac gacctgggcg 12000 tttatcgcaa cgagcgcatc cacaaggccg tgagcgtgag ccggcggcgc gagctcagcg 12060 accgcgagct gatgcacagc ctgcaaaggg ccctggctgg cacgggcagc ggcgatagag 12120 aggccgagtc ctactttgac gcgggcgctg acctgcgctg ggccccaagc cgacgcgccc 12180 tggaggcagc tggggccgga cctgggctgg cggtggcacc cgcgcgcgct ggcaacgtcg 12240 gcggcgtgga ggaatatgac gaggacgatg agtacgagcc agaggacggc gagtactaag 12300 cggtgatgtt tctgatcaga tgatgcaaga cgcaacggac ccggcggtgc gggcggcgct 12360 gcagagccag ccgtccggcc ttaactccac ggacgactgg cgccaggtca tggaccgcat 12420 catgtcgctg actgcgcgca atcctgacgc gttccggcag cagccgcagg ccaaccggct 12480 ctccgcaatt ctggaagcgg tggtcccggc gcgcgcaaac cccacgcacg agaaggtgct 12540 ggcgatcgta aacgcgctgg ccgaaaacag ggccatccgg cccgacgagg ccggcctggt 12600 ctacgacgcg ctgcttcagc gcgtggctcg ttacaacagc ggcaacgtgc agaccaacct 12660 ggaccggctg gtgggggatg tgcgcgaggc cgtggcgcag cgtgagcgcg cgcagcagca 12720 gggcaacctg ggctccatgg ttgcactaaa cgccttcctg agtacacagc ccgccaacgt 12780 gccgcgggga caggaggact acaccaactt tgtgagcgca ctgcggctaa tggtgactga 12840 gacaccgcaa agtgaggtgt accagtctgg gccagactat tttttccaga ccagtagaca 12900 aggcctgcag accgtaaacc tgagccaggc tttcaaaaac ttgcaggggc tgtggggggt 12960 gcgggctccc acaggcgacc gcgcgaccgt gtctagcttg ctgacgccca actcgcgcct 13020 gttgctgctg ctaatagcgc ccttcacgga cagtggcagc gtgtcccggg acacatacct 13080 aggtcacttg ctgacactgt accgcgaggc cataggtcag gcgcatgtgg acgagcatac 13140 tttccaggag attacaagtg tcagccgcgc gctggggcag gaggacacgg gcagcctgga 13200 ggcaacccta aactacctgc tgaccaaccg gcggcagaag atcccctcgt tgcacagttt 13260 aaacagcgag gaggagcgca ttttgcgcta cgtgcagcag agcgtgagcc ttaacctgat 13320 gcgcgacggg gtaacgccca gcgtggcgct ggacatgacc gcgcgcaaca tggaaccggg 13380 catgtatgcc tcaaaccggc cgtttatcaa ccgcctaatg gactacttgc atcgcgcggc 13440 cgccgtgaac cccgagtatt tcaccaatgc catcttgaac ccgcactggc taccgccccc 13500 tggtttctac accgggggat tcgaggtgcc cgagggtaac gatggattcc tctgggacga 13560 catagacgac agcgtgtttt ccccgcaacc gcagaccctg ctagagttgc aacagcgcga 13620 gcaggcagag gcggcgctgc gaaaggaaag cttccgcagg ccaagcagct tgtccgatct 13680 aggcgctgcg gccccgcggt cagatgctag tagcccattt ccaagcttga tagggtctct 13740 taccagcact cgcaccaccc gcccgcgcct gctgggcgag gaggagtacc taaacaactc 13800 gctgctgcag ccgcagcgcg aaaaaaacct gcctccggca tttcccaaca acgggataga 13860 gagcctagtg gacaagatga gtagatggaa gacgtacgcg caggagcaca gggacgtgcc 13920 aggcccgcgc ccgcccaccc gtcgtcaaag gcacgaccgt cagcggggtc tggtgtggga 13980 ggacgatgac tcggcagacg acagcagcgt cctggatttg ggagggagtg gcaacccgtt 14040 tgcgcacctt cgccccaggc tggggagaat gttttaaaaa aaaaaaaaaa gcatgatgca 14100 aaataaaaaa ctcaccaagg ccatggcacc gagcgttggt tttcttgtat tccccttagt 14160 atgcggcgcg cggcgatgta tgaggaaggt cctcctccct cctacgagag tgtggtgagc 14220 gcggcgccag tggcggcggc gctgggttct cccttcgatg ctcccctgga cccgccgttt 14280 gtgcctccgc ggtacctgcg gcctaccggg gggagaaaca gcatccgtta ctctgagttg 14340 gcacccctat tcgacaccac ccgtgtgtac ctggtggaca acaagtcaac ggatgtggca 14400 tccctgaact accagaacga ccacagcaac tttctgacca cggtcattca aaacaatgac 14460 tacagcccgg gggaggcaag cacacagacc atcaatcttg acgaccggtc gcactggggc 14520 ggcgacctga aaaccatcct gcataccaac atgccaaatg tgaacgagtt catgtttacc 14580 aataagttta aggcgcgggt gatggtgtcg cgcttgccta ctaaggacaa tcaggtggag 14640 ctgaaatacg agtgggtgga gttcacgctg cccgagggca actactccga gaccatgacc 14700 atagacctta tgaacaacgc gatcgtggag cactacttga aagtgggcag acagaacggg 14760 gttctggaaa gcgacatcgg ggtaaagttt gacacccgca acttcagact ggggtttgac 14820 cccgtcactg gtcttgtcat gcctggggta tatacaaacg aagccttcca tccagacatc 14880 attttgctgc caggatgcgg ggtggacttc acccacagcc gcctgagcaa cttgttgggc 14940 atccgcaagc ggcaaccctt ccaggagggc tttaggatca cctacgatga tctggagggt 15000 ggtaacattc ccgcactgtt ggatgtggac gcctaccagg cgagcttgaa agatgacacc 15060 gaacagggcg ggggtggcgc aggcggcagc aacagcagtg gcagcggcgc ggaagagaac 15120 tccaacgcgg cagccgcggc aatgcagccg gtggaggaca tgaacgatca tgccattcgc 15180 ggcgacacct ttgccacacg ggctgaggag aagcgcgctg aggccgaagc agcggccgaa 15240 gctgccgccc ccgctgcgca acccgaggtc gagaagcctc agaagaaacc ggtgatcaaa 15300 cccctgacag aggacagcaa gaaacgcagt tacaacctaa taagcaatga cagcaccttc 15360 acccagtacc gcagctggta ccttgcatac aactacggcg accctcagac cggaatccgc 15420 tcatggaccc tgctttgcac tcctgacgta acctgcggct cggagcaggt ctactggtcg 15480 ttgccagaca tgatgcaaga ccccgtgacc ttccgctcca cgcgccagat cagcaacttt 15540 ccggtggtgg gcgccgagct gttgcccgtg cactccaaga gcttctacaa cgaccaggcc 15600 gtctactccc aactcatccg ccagtttacc tctctgaccc acgtgttcaa tcgctttccc 15660 gagaaccaga ttttggcgcg cccgccagcc cccaccatca ccaccgtcag tgaaaacgtt 15720 cctgctctca cagatcacgg gacgctaccg ctgcgcaaca gcatcggagg agtccagcga 15780 gtgaccatta ctgacgccag acgccgcacc tgcccctacg tttacaaggc cctgggcata 15840 gtctcgccgc gcgtcctatc gagccgcact ttttgagcaa gcatgtccat ccttatatcg 15900 cccagcaata acacaggctg gggcctgcgc ttcccaagca agatgtttgg cggggccaag 15960 aagcgctccg accaacaccc agtgcgcgtg cgcgggcact accgcgcgcc ctggggcgcg 16020 cacaaacgcg gccgcactgg gcgcaccacc gtcgatgacg ccatcgacgc ggtggtggag 16080 gaggcgcgca actacacgcc cacgccgcca ccagtgtcca cagtggacgc ggccattcag 16140 accgtggtgc gcggagcccg gcgctatgct aaaatgaaga gacggcggag gcgcgtagca 16200 cgtcgccacc gccgccgacc cggcactgcc gcccaacgcg cggcggcggc cctgcttaac 16260 cgcgcacgtc gcaccggccg acgggcggcc atgcgggccg ctcgaaggct ggccgcgggt 16320 attgtcactg tgccccccag gtccaggcga cgagcggccg ccgcagcagc cgcggccatt 16380 agtgctatga ctcagggtcg caggggcaac gtgtattggg tgcgcgactc ggttagcggc 16440 ctgcgcgtgc ccgtgcgcac ccgccccccg cgcaactaga ttgcaagaaa aaactactta 16500 gactcgtact gttgtatgta tccagcggcg gcggcgcgca acgaagctat gtccaagcgc 16560 aaaatcaaag aagagatgct ccaggtcatc gcgccggaga tctatggccc cccgaagaag 16620 gaagagcagg attacaagcc ccgaaagcta aagcgggtca aaaagaaaaa gaaagatgat 16680 gatgatgaac ttgacgacga ggtggaactg ctgcacgcta ccgcgcccag gcgacgggta 16740 cagtggaaag gtcgacgcgt aaaacgtgtt ttgcgacccg gcaccaccgt agtctttacg 16800 cccggtgagc gctccacccg cacctacaag cgcgtgtatg atgaggtgta cggcgacgag 16860 gacctgcttg agcaggccaa cgagcgcctc ggggagtttg cctacggaaa gcggcataag 16920 gacatgctgg cgttgccgct ggacgagggc aacccaacac ctagcctaaa gcccgtaaca 16980 ctgcagcagg tgctgcccgc gcttgcaccg tccgaagaaa agcgcggcct aaagcgcgag 17040 tctggtgact tggcacccac cgtgcagctg atggtaccca agcgccagcg actggaagat 17100 gtcttggaaa aaatgaccgt ggaacctggg ctggagcccg aggtccgcgt gcggccaatc 17160 aagcaggtgg cgccgggact gggcgtgcag accgtggacg ttcagatacc cactaccagt 17220 agcaccagta ttgccaccgc cacagagggc atggagacac aaacgtcccc ggttgcctca 17280 gcggtggcgg atgccgcggt gcaggcggtc gctgcggccg cgtccaagac ctctacggag 17340 gtgcaaacgg acccgtggat gtttcgcgtt tcagcccccc ggcgcccgcg ccgttcgagg 17400 aagtacggcg ccgccagcgc gctactgccc gaatatgccc tacatccttc cattgcgcct 17460 acccccggct atcgtggcta cacctaccgc cccagaagac gagcaactac ccgacgccga 17520 accaccactg gaacccgccg ccgccgtcgc cgtcgccagc ccgtgctggc cccgatttcc 17580 gtgcgcaggg tggctcgcga aggaggcagg accctggtgc tgccaacagc gcgctaccac 17640 cccagcatcg tttaaaagcc ggtctttgtg gttcttgcag atatggccct cacctgccgc 17700 ctccgtttcc cggtgccggg attccgagga agaatgcacc gtaggagggg catggccggc 17760 cacggcctga cgggcggcat gcgtcgtgcg caccaccggc ggcggcgcgc gtcgcaccgt 17820 cgcatgcgcg gcggtatcct gcccctcctt attccactga tcgccgcggc gattggcgcc 17880 gtgcccggaa ttgcatccgt ggccttgcag gcgcagagac actgattaaa aacaagttgc 17940 atgtggaaaa atcaaaataa aaagtctgga ctctcacgct cgcttggtcc tgtaactatt 18000 ttgtagaatg gaagacatca actttgcgtc tctggccccg cgacacggct cgcgcccgtt 18060 catgggaaac tggcaagata tcggcaccag caatatgagc ggtggcgcct tcagctgggg 18120 ctcgctgtgg agcggcatta aaaatttcgg ttccaccgtt aagaactatg gcagcaaggc 18180 ctggaacagc agcacaggcc agatgctgag ggataagttg aaagagcaaa atttccaaca 18240 aaaggtggta gatggcctgg cctctggcat tagcggggtg gtggacctgg ccaaccaggc 18300 agtgcaaaat aagattaaca gtaagcttga tccccgccct cccgtagagg agcctccacc 18360 ggccgtggag acagtgtctc cagaggggcg tggcgaaaag cgtccgcgcc ccgacaggga 18420 agaaactctg gtgacgcaaa tagacgagcc tccctcgtac gaggaggcac taaagcaagg 18480 cctgcccacc acccgtccca tcgcgcccat ggctaccgga gtgctgggcc agcacacacc 18540 cgtaacgctg gacctgcctc cccccgccga cacccagcag aaacctgtgc tgccaggccc 18600 gaccgccgtt gttgtaaccc gtcctagccg cgcgtccctg cgccgcgccg ccagcggtcc 18660 gcgatcgttg cggcccgtag ccagtggcaa ctggcaaagc acactgaaca gcatcgtggg 18720 tctgggggtg caatccctga agcgccgacg atgcttctga tagctaacgt gtcgtatgtg 18780 tgtcatgtat gcgtccatgt cgccgccaga ggagctgctg agccgccgcg cgcccgcttt 18840 ccaagatggc taccccttcg atgatgccgc agtggtctta catgcacatc tcgggccagg 18900 acgcctcgga gtacctgagc cccgggctgg tgcagtttgc ccgcgccacc gagacgtact 18960 tcagcctgaa taacaagttt agaaacccca cggtggcgcc tacgcacgac gtgaccacag 19020 accggtccca gcgtttgacg ctgcggttca tccctgtgga ccgtgaggat actgcgtact 19080 cgtacaaggc gcggttcacc ctagctgtgg gtgataaccg tgtgctggac atggcttcca 19140 cgtactttga catccgcggc gtgctggaca ggggccctac ttttaagccc tactctggca 19200 ctgcctacaa cgccctggct cccaagggtg ccccaaatcc ttgcgaatgg gatgaagctg 19260 ctactgctct tgaaataaac ctagaagaag aggacgatga caacgaagac gaagtagacg 19320 agcaagctga gcagcaaaaa actcacgtat ttgggcaggc gccttattct ggtataaata 19380 ttacaaagga gggtattcaa ataggtgtcg aaggtcaaac acctaaatat gccgataaaa 19440 catttcaacc tgaacctcaa ataggagaat ctcagtggta cgaaacagaa attaatcatg 19500 cagctgggag agtcctaaaa aagactaccc caatgaaacc atgttacggt tcatatgcaa 19560 aacccacaaa tgaaaatgga gggcaaggca ttcttgtaaa gcaacaaaat ggaaagctag 19620 aaagtcaagt ggaaatgcaa tttttctcaa ctactgaggc agccgcaggc aatggtgata 19680 acttgactcc taaagtggta ttgtacagtg aagatgtaga tatagaaacc ccagacactc 19740 atatttctta catgcccact attaaggaag gtaactcacg agaactaatg ggccaacaat 19800 ctatgcccaa caggcctaat tacattgctt ttagggacaa ttttattggt ctaatgtatt 19860 acaacagcac gggtaatatg ggtgttctgg cgggccaagc atcgcagttg aatgctgttg 19920 tagatttgca agacagaaac acagagcttt cataccagct tttgcttgat tccattggtg 19980 atagaaccag gtacttttct atgtggaatc aggctgttga cagctatgat ccagatgtta 20040 gaattattga aaatcatgga actgaagatg aacttccaaa ttactgcttt ccactgggag 20100 gtgtgattaa tacagagact cttaccaagg taaaacctaa aacaggtcag gaaaatggat 20160 gggaaaaaga tgctacagaa ttttcagata aaaatgaaat aagagttgga aataattttg 20220 ccatggaaat caatctaaat gccaacctgt ggagaaattt cctgtactcc aacatagcgc 20280 tgtatttgcc cgacaagcta aagtacagtc cttccaacgt aaaaatttct gataacccaa 20340 acacctacga ctacatgaac aagcgagtgg tggctcccgg gctagtggac tgctacatta 20400 accttggagc acgctggtcc cttgactata tggacaacgt caacccattt aaccaccacc 20460 gcaatgctgg cctgcgctac cgctcaatgt tgctgggcaa tggtcgctat gtgcccttcc 20520 acatccaggt gcctcagaag ttctttgcca ttaaaaacct ccttctcctg ccgggctcat 20580 acacctacga gtggaacttc aggaaggatg ttaacatggt tctgcagagc tccctaggaa 20640 atgacctaag ggttgacgga gccagcatta agtttgatag catttgcctt tacgccacct 20700 tcttccccat ggcccacaac accgcctcca cgcttgaggc catgcttaga aacgacacca 20760 acgaccagtc ctttaacgac tatctctccg ccgccaacat gctctaccct atacccgcca 20820 acgctaccaa cgtgcccata tccatcccct cccgcaactg ggcggctttc cgcggctggg 20880 ccttcacgcg ccttaagact aaggaaaccc catcactggg ctcgggctac gacccttatt 20940 acacctactc tggctctata ccctacctag atggaacctt ttacctcaac cacaccttta 21000 agaaggtggc cattaccttt gactcttctg tcagctggcc tggcaatgac cgcctgctta 21060 cccccaacga gtttgaaatt aagcgctcag ttgacgggga gggttacaac gttgcccagt 21120 gtaacatgac caaagactgg ttcctggtac aaatgctagc taactataac attggctacc 21180 agggcttcta tatcccagag agctacaagg accgcatgta ctccttcttt agaaacttcc 21240 agcccatgag ccgtcaggtg gtggatgata ctaaatacaa ggactaccaa caggtgggca 21300 tcctacacca acacaacaac tctggatttg ttggctacct tgcccccacc atgcgcgaag 21360 gacaggccta ccctgctaac ttcccctatc cgcttatagg caagaccgca gttgacagca 21420 ttacccagaa aaagtttctt tgcgatcgca ccctttggcg catcccattc tccagtaact 21480 ttatgtccat gggcgcactc acagacctgg gccaaaacct tctctacgcc aactccgccc 21540 acgcgctaga catgactttt gaggtggatc ccatggacga gcccaccctt ctttatgttt 21600 tgtttgaagt ctttgacgtg gtccgtgtgc accagccgca ccgcggcgtc atcgaaaccg 21660 tgtacctgcg cacgcccttc tcggccggca acgccacaac ataaagaagc aagcaacatc 21720 aacaacagct gccgccatgg gctccagtga gcaggaactg aaagccattg tcaaagatct 21780 tggttgtggg ccatattttt tgggcaccta tgacaagcgc tttccaggct ttgtttctcc 21840 acacaagctc gcctgcgcca tagtcaatac ggccggtcgc gagactgggg gcgtacactg 21900 gatggccttt gcctggaacc cgcactcaaa aacatgctac ctctttgagc cctttggctt 21960 ttctgaccag cgactcaagc aggtttacca gtttgagtac gagtcactcc tgcgccgtag 22020 cgccattgct tcttcccccg accgctgtat aacgctggaa aagtccaccc aaagcgtaca 22080 ggggcccaac tcggccgcct gtggactatt ctgctgcatg tttctccacg cctttgccaa 22140 ctggccccaa actcccatgg atcacaaccc caccatgaac cttattaccg gggtacccaa 22200 ctccatgctc aacagtcccc aggtacagcc caccctgcgt cgcaaccagg aacagctcta 22260 cagcttcctg gagcgccact cgccctactt ccgcagccac agtgcgcaga ttaggagcgc 22320 cacttctttt tgtcacttga aaaacatgta aaaataatgt actagagaca ctttcaataa 22380 aggcaaatgc ttttatttgt acactctcgg gtgattattt acccccaccc ttgccgtctg 22440 cgccgtttaa aaatcaaagg ggttctgccg cgcatcgcta tgcgccactg gcagggacac 22500 gttgcgatac tggtgtttag tgctccactt aaactcaggc acaaccatcc gcggcagctc 22560 ggtgaagttt tcactccaca ggctgcgcac catcaccaac gcgtttagca ggtcgggcgc 22620 cgatatcttg aagtcgcagt tggggcctcc gccctgcgcg cgcgagttgc gatacacagg 22680 gttgcagcac tggaacacta tcagcgccgg gtggtgcacg ctggccagca cgctcttgtc 22740 ggagatcaga tccgcgtcca ggtcctccgc gttgctcagg gcgaacggag tcaactttgg 22800 tagctgcctt cccaaaaagg gcgcgtgccc aggctttgag ttgcactcgc accgtagtgg 22860 catcaaaagg tgaccgtgcc cggtctgggc gttaggatac agcgcctgca taaaagcctt 22920 gatctgctta aaagccacct gagcctttgc gccttcagag aagaacatgc cgcaagactt 22980 gccggaaaac tgattggccg gacaggccgc gtcgtgcacg cagcaccttg cgtcggtgtt 23040 ggagatctgc accacatttc ggccccaccg gttcttcacg atcttggcct tgctagactg 23100 ctccttcagc gcgcgctgcc cgttttcgct cgtcacatcc atttcaatca cgtgctcctt 23160 atttatcata atgcttccgt gtagacactt aagctcgcct tcgatctcag cgcagcggtg 23220 cagccacaac gcgcagcccg tgggctcgtg atgcttgtag gtcacctctg caaacgactg 23280 caggtacgcc tgcaggaatc gccccatcat cgtcacaaag gtcttgttgc tggtgaaggt 23340 cagctgcaac ccgcggtgct cctcgttcag ccaggtcttg catacggccg ccagagcttc 23400 cacttggtca ggcagtagtt tgaagttcgc ctttagatcg ttatccacgt ggtacttgtc 23460 catcagcgcg cgcgcagcct ccatgccctt ctcccacgca gacacgatcg gcacactcag 23520 cgggttcatc accgtaattt cactttccgc ttcgctgggc tcttcctctt cctcttgcgt 23580 ccgcatacca cgcgccactg ggtcgtcttc attcagccgc cgcactgtgc gcttacctcc 23640 tttgccatgc ttgattagca ccggtgggtt gctgaaaccc accatttgta gcgccacatc 23700 ttctctttct tcctcgctgt ccacgattac ctctggtgat ggcgggcgct cgggcttggg 23760 agaagggcgc ttctttttct tcttgggcgc aatggccaaa tccgccgccg aggtcgatgg 23820 ccgcgggctg ggtgtgcgcg gcaccagcgc gtcttgtgat gagtcttcct cgtcctcgga 23880 ctcgatacgc cgcctcatcc gcttttttgg gggcgcccgg ggaggcggcg gcgacgggga 23940 cggggacgac acgtcctcca tggttggggg acgtcgcgcc gcaccgcgtc cgcgctcggg 24000 ggtggtttcg cgctgctcct cttcccgact ggccatttcc ttctcctata ggcagaaaaa 24060 gatcatggag tcagtcgaga agaaggacag cctaaccgcc ccctctgagt tcgccaccac 24120 cgcctccacc gatgccgcca acgcgcctac caccttcccc gtcgaggcac ccccgcttga 24180 ggaggaggaa gtgattatcg agcaggaccc aggttttgta agcgaagacg acgaggaccg 24240 ctcagtacca acagaggata aaaagcaaga ccaggacaac gcagaggcaa acgaggaaca 24300 agtcgggcgg ggggacgaaa ggcatggcga ctacctagat gtgggagacg acgtgctgtt 24360 gaagcatctg cagcgccagt gcgccattat ctgcgacgcg ttgcaagagc gcagcgatgt 24420 gcccctcgcc atagcggatg tcagccttgc ctacgaacgc cacctattct caccgcgcgt 24480 accccccaaa cgccaagaaa acggcacatg cgagcccaac ccgcgcctca acttctaccc 24540 cgtatttgcc gtgccagagg tgcttgccac ctatcacatc tttttccaaa actgcaagat 24600 acccctatcc tgccgtgcca accgcagccg agcggacaag cagctggcct tgcggcaggg 24660 cgctgtcata cctgatatcg cctcgctcaa cgaagtgcca aaaatctttg agggtcttgg 24720 acgcgacgag aagcgcgcgg caaacgctct gcaacaggaa aacagcgaaa atgaaagtca 24780 ctctggagtg ttggtggaac tcgagggtga caacgcgcgc ctagccgtac taaaacgcag 24840 catcgaggtc acccactttg cctacccggc acttaaccta ccccccaagg tcatgagcac 24900 agtcatgagt gagctgatcg tgcgccgtgc gcagcccctg gagagggatg caaatttgca 24960 agaacaaaca gaggagggcc tacccgcagt tggcgacgag cagctagcgc gctggcttca 25020 aacgcgcgag cctgccgact tggaggagcg acgcaaacta atgatggccg cagtgctcgt 25080 taccgtggag cttgagtgca tgcagcggtt ctttgctgac ccggagatgc agcgcaagct 25140 agaggaaaca ttgcactaca cctttcgaca gggctacgta cgccaggcct gcaagatctc 25200 caacgtggag ctctgcaacc tggtctccta ccttggaatt ttgcacgaaa accgccttgg 25260 gcaaaacgtg cttcattcca cgctcaaggg cgaggcgcgc cgcgactacg tccgcgactg 25320 cgtttactta tttctatgct acacctggca gacggccatg ggcgtttggc agcagtgctt 25380 ggaggagtgc aacctcaagg agctgcagaa actgctaaag caaaacttga aggacctatg 25440 gacggccttc aacgagcgct ccgtggccgc gcacctggcg gacatcattt tccccgaacg 25500 cctgcttaaa accctgcaac agggtctgcc agacttcacc agtcaaagca tgttgcagaa 25560 ctttaggaac tttatcctag agcgctcagg aatcttgccc gccacctgct gtgcacttcc 25620 tagcgacttt gtgcccatta agtaccgcga atgccctccg ccgctttggg gccactgcta 25680 ccttctgcag ctagccaact accttgccta ccactctgac ataatggaag acgtgagcgg 25740 tgacggtcta ctggagtgtc actgtcgctg caacctatgc accccgcacc gctccctggt 25800 ttgcaattcg cagctgctta acgaaagtca aattatcggt acctttgagc tgcagggtcc 25860 ctcgcctgac gaaaagtccg cggctccggg gttgaaactc actccggggc tgtggacgtc 25920 ggcttacctt cgcaaatttg tacctgagga ctaccacgcc cacgagatta ggttctacga 25980 agaccaatcc cgcccgccta atgcggagct taccgcctgc gtcattaccc agggccacat 26040 tcttggccaa ttgcaagcca tcaacaaagc ccgccaagag tttctgctac gaaagggacg 26100 gggggtttac ttggaccccc agtccggcga ggagctcaac ccaatccccc cgccgccgca 26160 gccctatcag cagcagccgc gggcccttgc ttcccaggat ggcacccaaa aagaagctgc 26220 agctgccgcc gccacccacg gacgaggagg aatactggga cagtcaggca gaggaggttt 26280 tggacgagga ggaggaggac atgatggaag actgggagag cctagacgag gaagcttccg 26340 aggtcgaaga ggtgtcagac gaaacaccgt caccctcggt cgcattcccc tcgccggcgc 26400 cccagaaatc ggcaaccggt tccagcatgg ctacaacctc cgctcctcag gcgccgccgg 26460 cactgcccgt tcgccgaccc aaccgtagat gggacaccac tggaaccagg gccggtaagt 26520 ccaagcagcc gccgccgtta gcccaagagc aacaacagcg ccaaggctac cgctcatggc 26580 gcgggcacaa gaacgccata gttgcttgct tgcaagactg tgggggcaac atctccttcg 26640 cccgccgctt tcttctctac catcacggcg tggccttccc ccgtaacatc ctgcattact 26700 accgtcatct ctacagccca tactgcaccg gcggcagcgg cagcaacagc agcggccaca 26760 cagaagcaaa ggcgaccgga tagcaagact ctgacaaagc ccaagaaatc cacagcggcg 26820 gcagcagcag gaggaggagc gctgcgtctg gcgcccaacg aacccgtatc gacccgcgag 26880 cttagaaaca ggatttttcc cactctgtat gctatatttc aacagagcag gggccaagaa 26940 caagagctga aaataaaaaa caggtctctg cgatccctca cccgcagctg cctgtatcac 27000 aaaagcgaag atcagcttcg gcgcacgctg gaagacgcgg aggctctctt cagtaaatac 27060 tgcgcgctga ctcttaagga ctagtttcgc gccctttctc aaatttaagc gcgaaaacta 27120 cgtcatctcc agcggccaca cccggcgcca gcacctgtcg tcagcgccat ttatgagcaa 27180 ggaaattccc acgccctaca tgtggagtta ccagccacaa atgggacttg cggctggagc 27240 tgcccaagac tactcaaccc gaataaacta catgagcgcg ggaccccaca tgatatcccg 27300 ggtcaacgga atacgcgccc accgaaaccg aattctcctg gaacaggcgg ctattaccac 27360 cacacctcgt aataacctta atccccgtag ttggcccgct gccctggtgt accaggaaag 27420 tcccgctccc accactgtgg tacttcccag agacgcccag gccgaagttc agatgactaa 27480 ctcaggggcg cagcttgcgg gcggctttcg tcacagggtg cggtcgcccg ggcagggtat 27540 aactcacctg acaatcagag ggcgaggtat tcagctcaac gacgagtcgg tgagctcctc 27600 gcttggtctc cgtccggacg ggacatttca gatcggcggc gccggccgct cttcattcac 27660 gcctcgtcag gcaatcctaa ctctgcagac ctcgtcctct gagccgcgct ctggaggcat 27720 tggaactctg caatttattg aggagtttgt gccatcggtc tactttaacc ccttctcggg 27780 acctcccggc cactatccgg atcaatttat tcctaacttt gacgcggtaa aggactcggc 27840 ggacggctac gactgaatgt taagtggaga ggcagagcaa ctgcgcctga aacacctggt 27900 ccactgtcgc cgccacaagt gctttgcccg cgactccggt gagttttgct actttgaatt 27960 gcccgaggat catatcgagg gcccggcgca cggcgtccgg cttaccgccc agggagagct 28020 tgcccgtagc ctgattcggg agtttaccca gcgccccctg ctagttgagc gggacagggg 28080 accctgtgtt ctcactgtga tttgcaactg tcctaaccct ggattacatc aagatctttg 28140 ttgccatctc tgtgctgagt ataataaata cagaaattaa aatatactgg ggctcctatc 28200 gccatcctgt aaacgccacc gtcttcaccc gcccaagcaa accaaggcga accttacctg 28260 gtacttttaa catctctccc tctgtgattt acaacagttt caacccagac ggagtgagtc 28320 tacgagagaa cctctccgag ctcagctact ccatcagaaa aaacaccacc ctccttacct 28380 gccgggaacg tacgagtgcg tcaccggccg ctgcaccaca cctaccgcct gaccgtaaac 28440 cagacttttt ccggacagac ctcaataact ctgtttacca gaacaggagg tgagcttaga 28500 aaacccttag ggtattaggc caaaggcgca gctactgtgg ggtttatgaa caattcaagc 28560 aactctacgg gctattctaa ttcaggtttc tctagaatcg gggttggggt tattctctgt 28620 cttgtgattc tctttattct tatactaacg cttctctgcc taaggctcgc cgcctgctgt 28680 gtgcacattt gcatttattg tcagcttttt aaacgctggg gtcgccaccc aagatgatta 28740 ggtacataat cctaggttta ctcacccttg cgtcagccca cggtaccacc caaaaggtgg 28800 attttaagga gccagcctgt aatgttacat tcgcagctga agctaatgag tgcaccactc 28860 ttataaaatg caccacagaa catgaaaagc tgcttattcg ccacaaaaac aaaattggca 28920 agtatgctgt ttatgctatt tggcagccag gtgacactac agagtataat gttacagttt 28980 tccagggtaa aagtcataaa acttttatgt atacttttcc attttatgaa atgtgcgaca 29040 ttaccatgta catgagcaaa cagtataagt tgtggccccc acaaaattgt gtggaaaaca 29100 ctggcacttt ctgctgcact gctatgctaa ttacagtgct cgctttggtc tgtaccctac 29160 tctatattaa atacaaaagc agacgcagct ttattgagga aaagaaaatg ccttaattta 29220 ctaagttaca aagctaatgt caccactaac tgctttactc gctgcttgca aaacaaattc 29280 aaaaagttag cattataatt agaataggat ttaaaccccc cggtcatttc ctgctcaata 29340 ccattcccct gaacaattga ctctatgtgg gatatgctcc agcgctacaa ccttgaagtc 29400 aggcttcctg gatgtcagca tctgactttg gccagcacct gtcccgcgga tttgttccag 29460 tccaactaca gcgacccacc ctaacagaga tgaccaacac aaccaacgcg gccgccgcta 29520 ccggacttac atctaccaca aatacacccc aagtttctgc ctttgtcaat aactgggata 29580 acttgggcat gtggtggttc tccatagcgc ttatgtttgt atgccttatt attatgtggc 29640 tcatctgctg cctaaagcgc aaacgcgccc gaccacccat ctatagtccc atcattgtgc 29700 tacacccaaa caatgatgga atccatagat tggacggact gaaacacatg ttcttttctc 29760 ttacagtatg attaaatgag acatgattcc tcgagttttt atattactga cccttgttgc 29820 gcttttttgt gcgtgctcca cattggctgc ggtttctcac atcgaagtag actgcattcc 29880 agccttcaca gtctatttgc tttacggatt tgtcaccctc acgctcatct gcagcctcat 29940 cactgtggtc atcgccttta tccagtgcat tgactgggtc tgtgtgcgct ttgcatatct 30000 cagacaccat ccccagtaca gggacaggac tatagctgag cttcttagaa ttctttaatt 30060 atgaaattta ctgtgacttt tctgctgatt atttgcaccc tatctgcgtt ttgttccccg 30120 acctccaagc ctcaaagaca tatatcatgc agattcactc gtatatggaa tattccaagt 30180 tgctacaatg aaaaaagcga tctttccgaa gcctggttat atgcaatcat ctctgttatg 30240 gtgttctgca gtaccatctt agccctagct atatatccct accttgacat tggctggaac 30300 gcaatagatg ccatgaacca cccaactttc cccgcgcccg ctatgcttcc actgcaacaa 30360 gttgttgccg gcggctttgt cccagccaat cagcctcgcc caccttctcc cacccccact 30420 gaaatcagct actttaatct aacaggagga gatgactgac accctagatc tagaaatgga 30480 cggaattatt acagagcagc gcctgctaga aagacgcagg gcagcggccg agcaacagcg 30540 catgaatcaa gagctccaag acatggttaa cttgcaccag tgcaaaaggg gtatcttttg 30600 tctggtaaag caggccaaag tcacctacga cagtaatacc accggacacc gccttagcta 30660 caagttgcca accaagcgtc agaaattggt ggtcatggtg ggagaaaagc ccattaccat 30720 aactcagcac tcggtagaaa ccgaaggctg cattcactca ccttgtcaag gacctgagga 30780 tctctgcacc cttattaaga ccctgtgcgg tctcaaagat cttattccct ttaacggaag 30840 cggagctact aacttcagcc tgctgaagca ggctggtgac gtcgaggaga atcctggccc 30900 aatggtgagc aaaggcgaag agctgttcac cggcgtggtg cccatcctgg tggagctgga 30960 cggcgacgtg aacggccaca agttcagcgt gagcggcgag ggcgagggcg acgccaccta 31020 cggcaagctg accctgaagc tgctgtgcac caccggcaag ctgcccgtgc cctggcccac 31080 cctggtgacc accctgggct acggcgtgca gtgcttcgcc cggtaccccg accacatgaa 31140 gcagcacgac ttcttcaaga gcgccatgcc cgagggctac gtgcaggagc ggaccatctt 31200 cttcaaggac gacggcaact acaagacccg ggccgaggtg aagttcgagg gcgacaccct 31260 ggtgaaccgg atcgagctga agggcatcga cttcaaggag gacggcaaca tcctgggcca 31320 caagctggag tacaactaca acagccacaa cgtgtacatc accgccgaca agcagaagaa 31380 cggcatcaag gccaacttca agatccggca caacatcgag gacggcggcg tgcagctggc 31440 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 31500 ctacctgagc taccagagcg ccctgttcaa ggaccccaac gagaagcggg accacatggt 31560 gctgctggag ttcctgaccg ccgccggcat caccgagggc atgaacgagc tctataagta 31620 ataaaaaaaa ataataaagc atcacttact taaaatcagt tagcaaattt ctgtccagtt 31680 tattcagcag cacctccttg ccctcctccc agctctggta ttgcagcttc ctcctggctg 31740 caaactttct ccacaatcta aatggaatgt cagtttcctc ctgttcctgt ccatccgcac 31800 ccactatctt catgttgttg cagatgaagc gcgcaagacc gtctgaagat accttcaacc 31860 ccgtgtatcc atatgacacg gaaaccggtc ctccaactgt gccttttctt actcctccct 31920 ttgtatcccc caatgggttt caagagagtc cccctggggt actctctttg cgcctatccg 31980 aacctctagt tacctccaat ggcatgcttg cgctcaaaat gggcaacggc ctctctctgg 32040 acgaggccgg caaccttacc tcccaaaatg taaccactgt gagcccacct ctcaaaaaaa 32100 ccaagtcaaa cataaacctg gaaatatctg cacccctcac agttacctca gaagccctaa 32160 ctgtggctgc cgccgcacct ctaatggtcg cgggcaacac actcaccatg caatcacagg 32220 ccccgctaac cgtgcacgac tccaaactta gcattgccac ccaaggaccc ctcacagtgt 32280 cagaaggaaa gctagccctg caaacatcag gccccctcac caccaccgat agcagtaccc 32340 ttactatcac tgcctcaccc cctctaacta ctgccactgg tagcttgggc attgacttga 32400 aagagcccat ttatacacaa aatggaaaac taggactaaa gtacggggct cctttgcatg 32460 taacagacga cctaaacact ttgaccgtag caactggtcc aggtgtgact attaataata 32520 cttccttgca aactaaagtt actggagcct tgggttttga ttcacaaggc aatatgcaac 32580 ttaatgtagc aggaggacta aggattgatt ctcaaaacag acgccttata cttgatgtta 32640 gttatccgtt tgatgctcaa aaccaactaa atctaagact aggacagggc cctcttttta 32700 taaactcagc ccacaacttg gatattaact acaacaaagg cctttacttg tttacagctt 32760 caaacaattc caaaaagctt gaggttaacc taagcactgc caaggggttg atgtttgacg 32820 ctacagccat agccattaat gcaggagatg ggcttgaatt tggttcacct aatgcaccaa 32880 acacaaatcc cctcaaaaca aaaattggcc atggcctaga atttgattca aacaaggcta 32940 tggttcctaa actaggaact ggccttagtt ttgacagcac aggtgccatt acagtaggaa 33000 acaaaaataa tgataagcta actttgtgga ccacaccagc tccatctcct aactgtagac 33060 taaatgcaga gaaagatgct aaactcactt tggtcttaac aaaatgtggc agtcaaatac 33120 ttgctacagt ttcagttttg gctgttaaag gcagtttggc tccaatatct ggaacagttc 33180 aaagtgctca tcttattata agatttgacg aaaatggagt gctactaaac aattccttcc 33240 tggacccaga atattggaac tttagaaatg gagatcttac tgaaggcaca gcctatacaa 33300 acgctgttgg atttatgcct aacctatcag cttatccaaa atctcacggt aaaactgcca 33360 aaagtaacat tgtcagtcaa gtttacttaa acggagacaa aactaaacct gtaacactaa 33420 ccattacact aaacggtaca caggaaacag gagacacaac tccaagtgca tactctatgt 33480 cattttcatg ggactggtct ggccacaact acattaatga aatatttgcc acatcctctt 33540 acactttttc atacattgcc caagaataaa gaatcgtttg tgttatgttt caacgtgttt 33600 atttttcaat tgcagaaaat ttcgaatcat ttttcattca gtagtatagc cccaccacca 33660 catagcttat acagatcacc gtacctaaat caaactcaca gaaccctagt attcaacctg 33720 ccacctccct cccaacacac agagtacaca gtcctttctc cccggctggc cttaaaaagc 33780 atcatatcat gggtaacaga catattctta ggtgttatat tccacacggt ttcctgtcga 33840 gccaaacgct catcagtgat attaataaac tccccgggca gctcacttaa gttcatgtcg 33900 ctgtccagct gctgagccac aggctgctgt ccaacttgcg gttgcttaac gggcggcgaa 33960 ggagaagtcc acgcctacat gggggtagag tcataatcgt gcatcaggat agggcggtgg 34020 tgctgcagca gcgcgcgaat aaactgctgc cgccgccgct ccgtcctgca ggaatacaac 34080 atggcagtgg tctcctcagc gatgattcgc accgcccgca gcataaggcg ccttgtcctc 34140 cgggcacagc agcgcaccct gatctcactt aaatcagcac agtaactgca gcacagcacc 34200 acaatattgt tcaaaatccc acagtgcaag gcgctgtatc caaagctcat ggcggggacc 34260 acagaaccca cgtggccatc ataccacaag cgcaggtaga ttaagtggcg acccctcata 34320 aacacgctgg acataaacat tacctctttt ggcatgttgt aattcaccac ctcccggtac 34380 catataaacc tctgattaaa catggcgcca tccaccacca tcctaaacca gctggccaaa 34440 acctgcccgc cggctataca ctgcagggaa ccgggactgg aacaatgaca gtggagagcc 34500 caggactcgt aaccatggat catcatgctc gtcatgatat caatgttggc acaacacagg 34560 cacacgtgca tacacttcct caggattaca agctcctccc gcgttagaac catatcccag 34620 ggaacaaccc attcctgaat cagcgtaaat cccacactgc agggaagacc tcgcacgtaa 34680 ctcacgttgt gcattgtcaa agtgttacat tcgggcagca gcggatgatc ctccagtatg 34740 gtagcgcggg tttctgtctc aaaaggaggt agacgatccc tactgtacgg agtgcgccga 34800 gacaaccgag atcgtgttgg tcgtagtgtc atgccaaatg gaacgccgga cgtagtcata 34860 tttcctgaag caaaaccagg tgcgggcgtg acaaacagat ctgcgtctcc ggtctcgccg 34920 cttagatcgc tctgtgtagt agttgtagta tatccactct ctcaaagcat ccaggcgccc 34980 cctggcttcg ggttctatgt aaactccttc atgcgccgct gccctgataa catccaccac 35040 cgcagaataa gccacaccca gccaacctac acattcgttc tgcgagtcac acacgggagg 35100 agcgggaaga gctggaagaa ccatgttttt ttttttattc caaaagatta tccaaaacct 35160 caaaatgaag atctattaag tgaacgcgct cccctccggt ggcgtggtca aactctacag 35220 ccaaagaaca gataatggca tttgtaagat gttgcacaat ggcttccaaa aggcaaacgg 35280 ccctcacgtc caagtggacg taaaggctaa acccttcagg gtgaatctcc tctataaaca 35340 ttccagcacc ttcaaccatg cccaaataat tctcatctcg ccaccttctc aatatatctc 35400 taagcaaatc ccgaatatta agtccggcca ttgtaaaaat ctgctccaga gcgccctcca 35460 ccttcagcct caagcagcga atcatgattg caaaaattca ggttcctcac agacctgtat 35520 aagattcaaa agcggaacat taacaaaaat accgcgatcc cgtaggtccc ttcgcagggc 35580 cagctgaaca taatcgtgca ggtctgcacg gaccagcgcg gccacttccc cgccaggaac 35640 catgacaaaa gaacccacac tgattatgac acgcatactc ggagctatgc taaccagcgt 35700 agccccgatg taagcttgtt gcatgggcgg cgatataaaa tgcaaggtgc tgctcaaaaa 35760 atcaggcaaa gcctcgcgca aaaaagaaag cacatcgtag tcatgctcat gcagataaag 35820 gcaggtaagc tccggaacca ccacagaaaa agacaccatt tttctctcaa acatgtctgc 35880 gggtttctgc ataaacacaa aataaaataa caaaaaaaca tttaaacatt agaagcctgt 35940 cttacaacag gaaaaacaac ccttataagc ataagacgga ctacggccat gccggcgtga 36000 ccgtaaaaaa actggtcacc gtgattaaaa agcaccaccg acagctcctc ggtcatgtcc 36060 ggagtcataa tgtaagactc ggtaaacaca tcaggttgat tcacatcggt cagtgctaaa 36120 aagcgaccga aatagcccgg gggaatacat acccgcaggc gtagagacaa cattacagcc 36180 cccataggag gtataacaaa attaatagga gagaaaaaca cataaacacc tgaaaaaccc 36240 tcctgcctag gcaaaatagc accctcccgc tccagaacaa catacagcgc ttccacagcg 36300 gcagccataa cagtcagcct taccagtaaa aaagaaaacc tattaaaaaa acaccactcg 36360 acacggcacc agctcaatca gtcacagtgt aaaaaagggc caagtgcaga gcgagtatat 36420 ataggactaa aaaatgacgt aacggttaaa gtccacaaaa aacacccaga aaaccgcacg 36480 cgaacctacg cccagaaacg aaagccaaaa aacccacaac ttcctcaaat cgtcacttcc 36540 gttttcccac gttacgtcac ttcccatttt aagaaaacta caattcccaa cacatacaag 36600 ttactccgcc ctaaaaccta cgtcacccgc cccgttccca cgccccgcgc cacgtcacaa 36660 actccacccc ctcattatca tattggcttc aatccaaaat aaggtatatt attgatgatg 36720 36720 <210> 10 <211> 36790 <212> DNA <213> Artificial Sequence <220> <223> Synthetic adenovirus genome YPet-P2A-E4-ORF2 <400> 10 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420 cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480 tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540 tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600 aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660 tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720 cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780 gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840 cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900 ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960 cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020 caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080 ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140 tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200 gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260 ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320 cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380 ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440 gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500 cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560 ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620 gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680 cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740 ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800 tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860 gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920 caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980 gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040 agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100 aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160 cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220 gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280 gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340 gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400 gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460 tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520 ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580 tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640 agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700 tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760 gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820 acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880 gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940 aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000 ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060 gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120 tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180 acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240 aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300 tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360 gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420 tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480 ttggctctag cgatgaagat acagattgag gtactgaaat gtgtgggcgt ggcttaaggg 3540 tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg 3600 ccgccgccca acttttctat acaaagttgt aatgagcacc aactcgtttg atggaagcat 3660 tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat 3720 gggctccagc attgatggtc gccccgtcct gcccgcaaac tctactacct tgacctacga 3780 gaccgtgtct ggaacgccgt tggagactgc agcctccgcc gccgcttcag ccgctgcagc 3840 caccgcccgc gggattgtga ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc 3900 ttcccgttca tccgcccgcg atgacaagtt gacggctctt ttggcacaat tggattcttt 3960 gacccgggaa cttaatgtcg tttctcagca gctgttggat ctgcgccagc aggtttctgc 4020 cctgaaggct tcctcccctc ccaatgcggt ttaaaacata aataaaaaac cagactctgt 4080 ttggatttgg atcaagcata agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg 4140 taggcccggg accagcggtc tcggtcgttg agggtcctgt gtattttttc caggacgtgg 4200 taaaggtgac tctggatgtt cagatacatg ggcataagcc cgtctctggg gtggaggtag 4260 caccactgca gagcttcatg ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag 4320 cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc tgattgccag gggcaggccc 4380 ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt gcatacgtgg ggatatgaga 4440 tgcatcttgg actgtatttt taggttggct atgttcccag ccatatccct ccggggattc 4500 atgttgtgca gaaccaccag cacagtgtat ccggtgcact tgggaaattt gtcatgtagc 4560 ttagaaggaa atgcgtggaa gaacttggag acgcccttgt gacctccaag attttccatg 4620 cattcgtcca taatgatggc aatgggccca cgggcggcgg cctgggcgaa gatatttctg 4680 ggatcactaa cgtcatagtt gtgttccagg atgagatcgt cataggccat ttttacaaag 4740 cgcgggcgga gggtgccaga ctgcggtata atggttccat ccggcccagg ggcgtagtta 4800 ccctcacaga tttgcatttc ccacgctttg agttcagatg gggggatcat gtctacctgc 4860 ggggcgatga agaaaacggt ttccggggta ggggagatca gctgggaaga aagcaggttc 4920 ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa tcacacctat taccgggtgc 4980 aactggtagt taagagagct gcagctgccg tcatccctga gcaggggggc cacttcgtta 5040 agcatgtccc tgactcgcat gttttccctg accaaatccg ccagaaggcg ctcgccgccc 5100 agcgatagca gttcttgcaa ggaagcaaag tttttcaacg gtttgagacc gtccgccgta 5160 ggcatgcttt tgagcgtttg accaagcagt tccaggcggt cccacagctc ggtcacctgc 5220 tctacggcat ctcgatccag catatctcct cgtttcgcgg gttggggcgg ctttcgctgt 5280 acggcagtag tcggtgctcg tccagacggg ccagggtcat gtctttccac gggcgcaggg 5340 tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc tccgggctgc gcgctggcca 5400 gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt 5460 cggccaggta gcatttgacc atggtgtcat agtccagccc ctccgcggcg tggcccttgg 5520 cgcgcagctt gcccttggag gaggcgccgc acgaggggca gtgcagactt ttgagggcgt 5580 agagcttggg cgcgagaaat accgattccg gggagtaggc atccgcgccg caggccccgc 5640 agacggtctc gcattccacg agccaggtga gctctggccg ttcggggtca aaaaccaggt 5700 ttcccccatg ctttttgatg cgtttcttac ctctggtttc catgagccgg tgtccacgct 5760 cggtgacgaa aaggctgtcc gtgtccccgt atacagactt gagaggcctg tcctcgagcg 5820 gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacaaag gctcgcgtcc 5880 aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact agggggtcca 5940 ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag gtgattggtt 6000 tgtaggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag ggggtggggg 6060 cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt tggggtgagt 6120 actccctctg aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg 6180 aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgca tccatctggt 6240 cagaaaagac aatctttttg ttgtcaagct tggtggcaaa cgacccgtag agggcgttgg 6300 acagcaactt ggcgatggag cgcagggttt ggtttttgtc gcgatcggcg cgctccttgg 6360 ccgcgatgtt tagctgcacg tattcgcgcg caacgcaccg ccattcggga aagacggtgg 6420 tgcgctcgtc gggcaccagg tgcacgcgcc aaccgcggtt gtgcagggtg acaaggtcaa 6480 cgctggtggc tacctctccg cgtaggcgct cgttggtcca gcagaggcgg ccgcccttgc 6540 gcgagcagaa tggcggtagg gggtctagct gcgtctcgtc cggggggtct gcgtccacgg 6600 taaagacccc gggcagcagg cgcgcgtcga agtagtctat cttgcatcct tgcaagtcta 6660 gcgcctgctg ccatgcgcgg gcggcaagcg cgcgctcgta tgggttgagt gggggacccc 6720 atggcatggg gtgggtgagc gcggaggcgt acatgccgca aatgtcgtaa acgtagaggg 6780 gctctctgag tattccaaga tatgtagggt agcatcttcc accgcggatg ctggcgcgca 6840 cgtaatcgta tagttcgtgc gagggagcga ggaggtcggg accgaggttg ctacgggcgg 6900 gctgctctgc tcggaagact atctgcctga agatggcatg tgagttggat gatatggttg 6960 gacgctggaa gacgttgaag ctggcgtctg tgagacctac cgcgtcacgc acgaaggagg 7020 cgtaggagtc gcgcagcttg ttgaccagct cggcggtgac ctgcacgtct agggcgcagt 7080 agtccagggt ttccttgatg atgtcatact tatcctgtcc cttttttttc cacagctcgc 7140 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct 7200 ccgaacggta agagcctagc atgtagaact ggttgacggc ctggtaggcg cagcatccct 7260 tttctacggg tagcgcgtat gcctgcgcgg ccttccggag cgaggtgtgg gtgagcgcaa 7320 aggtgtccct gaccatgact ttgaggtact ggtatttgaa gtcagtgtcg tcgcatccgc 7380 cctgctccca gagcaaaaag tccgtgcgct ttttggaacg cggatttggc agggcgaagg 7440 tgacatcgtt gaagagtatc tttcccgcgc gaggcataaa gttgcgtgtg atgcggaagg 7500 gtcccggcac ctcggaacgg ttgttaatta cctgggcggc gagcacgatc tcgtcaaagc 7560 cgttgatgtt gtggcccaca atgtaaagtt ccaagaagcg cgggatgccc ttgatggaag 7620 gcaatttttt aagttcctcg taggtgagct cttcagggga gctgagcccg tgctctgaaa 7680 gggcccagtc tgcaagatga gggttggaag cgacgaatga gctccacagg tcacgggcca 7740 ttagcatttg caggtggtcg cgaaaggtcc taaactggcg acctatggcc attttttctg 7800 gggtgatgca gtagaaggta agcgggtctt gttcccagcg gtcccatcca aggttcgcgg 7860 ctaggtctcg cgcggcagtc actagaggct catctccgcc gaacttcatg accagcatga 7920 agggcacgag ctgcttccca aaggccccca tccaagtata ggtctctaca tcgtaggtga 7980 caaagagacg ctcggtgcga ggatgcgagc cgatcgggaa gaactggatc tcccgccacc 8040 aattggagga gtggctattg atgtggtgaa agtagaagtc cctgcgacgg gccgaacact 8100 cgtgctggct tttgtaaaaa cgtgcgcagt actggcagcg gtgcacgggc tgtacatcct 8160 gcacgaggtt gacctgacga ccgcgcacaa ggaagcagag tgggaatttg agcccctcgc 8220 ctggcgggtt tggctggtgg tcttctactt cggctgcttg tccttgaccg tctggctgct 8280 cgaggggagt tacggtggat cggaccacca cgccgcgcga gcccaaagtc cagatgtccg 8340 cgcgcggcgg tcggagcttg atgacaacat cgcgcagatg ggagctgtcc atggtctgga 8400 gctcccgcgg cgtcaggtca ggcgggagct cctgcaggtt tacctcgcat agacgggtca 8460 gggcgcgggc tagatccagg tgatacctaa tttccagggg ctggttggtg gcggcgtcga 8520 tggcttgcaa gaggccgcat ccccgcggcg cgactacggt accgcgcggc gggcggtggg 8580 ccgcgggggt gtccttggat gatgcatcta aaagcggtga cgcgggcgag cccccggagg 8640 tagggggggc tccggacccg ccgggagagg gggcaggggc acgtcggcgc cgcgcgcggg 8700 caggagctgg tgctgcgcgc gtaggttgct ggcgaacgcg acgacgcggc ggttgatctc 8760 ctgaatctgg cgcctctgcg tgaagacgac gggcccggtg agcttgagcc tgaaagagag 8820 ttcgacagaa tcaatttcgg tgtcgttgac ggcggcctgg cgcaaaatct cctgcacgtc 8880 tcctgagttg tcttgatagg cgatctcggc catgaactgc tcgatctctt cctcctggag 8940 atctccgcgt ccggctcgct ccacggtggc ggcgaggtcg ttggaaatgc gggccatgag 9000 ctgcgagaag gcgttgaggc ctccctcgtt ccagacgcgg ctgtagacca cgcccccttc 9060 ggcatcgcgg gcgcgcatga ccacctgcgc gagattgagc tccacgtgcc gggcgaagac 9120 ggcgtagttt cgcaggcgct gaaagaggta gttgagggtg gtggcggtgt gttctgccac 9180 gaagaagtac ataacccagc gtcgcaacgt ggattcgttg atatccccca aggcctcaag 9240 gcgctccatg gcctcgtaga agtccacggc gaagttgaaa aactgggagt tgcgcgccga 9300 cacggttaac tcctcctcca gaagacggat gagctcggcg acagtgtcgc gcacctcgcg 9360 ctcaaaggct acaggggcct cttcttcttc ttcaatctcc tcttccataa gggcctcccc 9420 ttcttcttct tctggcggcg gtgggggagg ggggacacgg cggcgacgac ggcgcaccgg 9480 gaggcggtcg acaaagcgct cgatcatctc cccgcggcga cggcgcatgg tctcggtgac 9540 ggcgcggccg ttctcgcggg ggcgcagttg gaagacgccg cccgtcatgt cccggttatg 9600 ggttggcggg gggctgccat gcggcaggga tacggcgcta acgatgcatc tcaacaattg 9660 ttgtgtaggt actccgccgc cgagggacct gagcgagtcc gcatcgaccg gatcggaaaa 9720 cctctcgaga aaggcgtcta accagtcaca gtcgcaaggt aggctgagca ccgtggcggg 9780 cggcagcggg cggcggtcgg ggttgtttct ggcggaggtg ctgctgatga tgtaattaaa 9840 gtaggcggtc ttgagacggc ggatggtcga cagaagcacc atgtccttgg gtccggcctg 9900 ctgaatgcgc aggcggtcgg ccatgcccca ggcttcgttt tgacatcggc gcaggtcttt 9960 gtagtagtct tgcatgagcc tttctaccgg cacttcttct tctccttcct cttgtcctgc 10020 atctcttgca tctatcgctg cggcggcggc ggagtttggc cgtaggtggc gccctcttcc 10080 tcccatgcgt gtgaccccga agcccctcat cggctgaagc agggctaggt cggcgacaac 10140 gcgctcggct aatatggcct gctgcacctg cgtgagggta gactggaagt catccatgtc 10200 cacaaagcgg tggtatgcgc ccgtgttgat ggtgtaagtg cagttggcca taacggacca 10260 gttaacggtc tggtgacccg gctgcgagag ctcggtgtac ctgagacgcg agtaagccct 10320 cgagtcaaat acgtagtcgt tgcaagtccg caccaggtac tggtatccca ccaaaaagtg 10380 cggcggcggc tggcggtaga ggggccagcg tagggtggcc ggggctccgg gggcgagatc 10440 ttccaacata aggcgatgat atccgtagat gtacctggac atccaggtga tgccggcggc 10500 ggtggtggag gcgcgcggaa agtcgcggac gcggttccag atgttgcgca gcggcaaaaa 10560 gtgctccatg gtcgggacgc tctggccggt caggcgcgcg caatcgttga cgctctagac 10620 cgtgcaaaag gagagcctgt aagcgggcac tcttccgtgg tctggtggat aaattcgcaa 10680 gggtatcatg gcggacgacc ggggttcgag ccccgtatcc ggccgtccgc cgtgatccat 10740 gcggttaccg cccgcgtgtc gaacccaggt gtgcgacgtc agacaacggg ggagtgctcc 10800 ttttggcttc cttccaggcg cggcggctgc tgcgctagct tttttggcca ctggccgcgc 10860 gcagcgtaag cggttaggct ggaaagcgaa agcattaagt ggctcgctcc ctgtagccgg 10920 agggttattt tccaagggtt gagtcgcggg acccccggtt cgagtctcgg accggccgga 10980 ctgcggcgaa cgggggtttg cctccccgtc atgcaagacc ccgcttgcaa attcctccgg 11040 aaacagggac gagccccttt tttgcttttc ccagatgcat ccggtgctgc ggcagatgcg 11100 cccccctcct cagcagcggc aagagcaaga gcagcggcag acatgcaggg caccctcccc 11160 tcctcctacc gcgtcaggag gggcgacatc cgcggttgac gcggcagcag atggtgatta 11220 cgaacccccg cggcgccggg cccggcacta cctggacttg gaggagggcg agggcctggc 11280 gcggctagga gcgccctctc ctgagcggta cccaagggtg cagctgaagc gtgatacgcg 11340 tgaggcgtac gtgccgcggc agaacctgtt tcgcgaccgc gagggagagg agcccgagga 11400 gatgcgggat cgaaagttcc acgcagggcg cgagctgcgg catggcctga atcgcgagcg 11460 gttgctgcgc gaggaggact ttgagcccga cgcgcgaacc gggattagtc ccgcgcgcgc 11520 acacgtggcg gccgccgacc tggtaaccgc atacgagcag acggtgaacc aggagattaa 11580 ctttcaaaaa agctttaaca accacgtgcg tacgcttgtg gcgcgcgagg aggtggctat 11640 aggactgatg catctgtggg actttgtaag cgcgctggag caaaacccaa atagcaagcc 11700 gctcatggcg cagctgttcc ttatagtgca gcacagcagg gacaacgagg cattcaggga 11760 tgcgctgcta aacatagtag agcccgaggg ccgctggctg ctcgatttga taaacatcct 11820 gcagagcata gtggtgcagg agcgcagctt gagcctggct gacaaggtgg ccgccatcaa 11880 ctattccatg cttagcctgg gcaagtttta cgcccgcaag atataccata ccccttacgt 11940 tcccatagac aaggaggtaa agatcgaggg gttctacatg cgcatggcgc tgaaggtgct 12000 taccttgagc gacgacctgg gcgtttatcg caacgagcgc atccacaagg ccgtgagcgt 12060 gagccggcgg cgcgagctca gcgaccgcga gctgatgcac agcctgcaaa gggccctggc 12120 tggcacgggc agcggcgata gagaggccga gtcctacttt gacgcgggcg ctgacctgcg 12180 ctgggcccca agccgacgcg ccctggaggc agctggggcc ggacctgggc tggcggtggc 12240 acccgcgcgc gctggcaacg tcggcggcgt ggaggaatat gacgaggacg atgagtacga 12300 gccagaggac ggcgagtact aagcggtgat gtttctgatc agatgatgca agacgcaacg 12360 gacccggcgg tgcgggcggc gctgcagagc cagccgtccg gccttaactc cacggacgac 12420 tggcgccagg tcatggaccg catcatgtcg ctgactgcgc gcaatcctga cgcgttccgg 12480 cagcagccgc aggccaaccg gctctccgca attctggaag cggtggtccc ggcgcgcgca 12540 aaccccacgc acgagaaggt gctggcgatc gtaaacgcgc tggccgaaaa cagggccatc 12600 cggcccgacg aggccggcct ggtctacgac gcgctgcttc agcgcgtggc tcgttacaac 12660 agcggcaacg tgcagaccaa cctggaccgg ctggtggggg atgtgcgcga ggccgtggcg 12720 cagcgtgagc gcgcgcagca gcagggcaac ctgggctcca tggttgcact aaacgccttc 12780 ctgagtacac agcccgccaa cgtgccgcgg ggacaggagg actacaccaa ctttgtgagc 12840 gcactgcggc taatggtgac tgagacaccg caaagtgagg tgtaccagtc tgggccagac 12900 tattttttcc agaccagtag acaaggcctg cagaccgtaa acctgagcca ggctttcaaa 12960 aacttgcagg ggctgtgggg ggtgcgggct cccacaggcg accgcgcgac cgtgtctagc 13020 ttgctgacgc ccaactcgcg cctgttgctg ctgctaatag cgcccttcac ggacagtggc 13080 agcgtgtccc gggacacata cctaggtcac ttgctgacac tgtaccgcga ggccataggt 13140 caggcgcatg tggacgagca tactttccag gagattacaa gtgtcagccg cgcgctgggg 13200 caggaggaca cgggcagcct ggaggcaacc ctaaactacc tgctgaccaa ccggcggcag 13260 aagatcccct cgttgcacag tttaaacagc gaggaggagc gcattttgcg ctacgtgcag 13320 cagagcgtga gccttaacct gatgcgcgac ggggtaacgc ccagcgtggc gctggacatg 13380 accgcgcgca acatggaacc gggcatgtat gcctcaaacc ggccgtttat caaccgccta 13440 atggactact tgcatcgcgc ggccgccgtg aaccccgagt atttcaccaa tgccatcttg 13500 aacccgcact ggctaccgcc ccctggtttc tacaccgggg gattcgaggt gcccgagggt 13560 aacgatggat tcctctggga cgacatagac gacagcgtgt tttccccgca accgcagacc 13620 ctgctagagt tgcaacagcg cgagcaggca gaggcggcgc tgcgaaagga aagcttccgc 13680 aggccaagca gcttgtccga tctaggcgct gcggccccgc ggtcagatgc tagtagccca 13740 tttccaagct tgatagggtc tcttaccagc actcgcacca cccgcccgcg cctgctgggc 13800 gaggaggagt acctaaacaa ctcgctgctg cagccgcagc gcgaaaaaaa cctgcctccg 13860 gcatttccca acaacgggat agagagccta gtggacaaga tgagtagatg gaagacgtac 13920 gcgcaggagc acagggacgt gccaggcccg cgcccgccca cccgtcgtca aaggcacgac 13980 cgtcagcggg gtctggtgtg ggaggacgat gactcggcag acgacagcag cgtcctggat 14040 ttgggaggga gtggcaaccc gtttgcgcac cttcgcccca ggctggggag aatgttttaa 14100 aaaaaaaaaa gcatgatgca aaataaaaaa ctcaccaagg ccatggcacc gagcgttggt 14160 tttcttgtat tccccttagt atgcggcgcg cggcgatgta tgaggaaggt cctcctccct 14220 cctacgagag tgtggtgagc gcggcgccag tggcggcggc gctgggttct cccttcgatg 14280 ctcccctgga cccgccgttt gtgcctccgc ggtacctgcg gcctaccggg gggagaaaca 14340 gcatccgtta ctctgagttg gcacccctat tcgacaccac ccgtgtgtac ctggtggaca 14400 acaagtcaac ggatgtggca tccctgaact accagaacga ccacagcaac tttctgacca 14460 cggtcattca aaacaatgac tacagcccgg gggaggcaag cacacagacc atcaatcttg 14520 acgaccggtc gcactggggc ggcgacctga aaaccatcct gcataccaac atgccaaatg 14580 tgaacgagtt catgtttacc aataagttta aggcgcgggt gatggtgtcg cgcttgccta 14640 ctaaggacaa tcaggtggag ctgaaatacg agtgggtgga gttcacgctg cccgagggca 14700 actactccga gaccatgacc atagacctta tgaacaacgc gatcgtggag cactacttga 14760 aagtgggcag acagaacggg gttctggaaa gcgacatcgg ggtaaagttt gacacccgca 14820 acttcagact ggggtttgac cccgtcactg gtcttgtcat gcctggggta tatacaaacg 14880 aagccttcca tccagacatc attttgctgc caggatgcgg ggtggacttc acccacagcc 14940 gcctgagcaa cttgttgggc atccgcaagc ggcaaccctt ccaggagggc tttaggatca 15000 cctacgatga tctggagggt ggtaacattc ccgcactgtt ggatgtggac gcctaccagg 15060 cgagcttgaa agatgacacc gaacagggcg ggggtggcgc aggcggcagc aacagcagtg 15120 gcagcggcgc ggaagagaac tccaacgcgg cagccgcggc aatgcagccg gtggaggaca 15180 tgaacgatca tgccattcgc ggcgacacct ttgccacacg ggctgaggag aagcgcgctg 15240 aggccgaagc agcggccgaa gctgccgccc ccgctgcgca acccgaggtc gagaagcctc 15300 agaagaaacc ggtgatcaaa cccctgacag aggacagcaa gaaacgcagt tacaacctaa 15360 taagcaatga cagcaccttc acccagtacc gcagctggta ccttgcatac aactacggcg 15420 accctcagac cggaatccgc tcatggaccc tgctttgcac tcctgacgta acctgcggct 15480 cggagcaggt ctactggtcg ttgccagaca tgatgcaaga ccccgtgacc ttccgctcca 15540 cgcgccagat cagcaacttt ccggtggtgg gcgccgagct gttgcccgtg cactccaaga 15600 gcttctacaa cgaccaggcc gtctactccc aactcatccg ccagtttacc tctctgaccc 15660 acgtgttcaa tcgctttccc gagaaccaga ttttggcgcg cccgccagcc cccaccatca 15720 ccaccgtcag tgaaaacgtt cctgctctca cagatcacgg gacgctaccg ctgcgcaaca 15780 gcatcggagg agtccagcga gtgaccatta ctgacgccag acgccgcacc tgcccctacg 15840 tttacaaggc cctgggcata gtctcgccgc gcgtcctatc gagccgcact ttttgagcaa 15900 gcatgtccat ccttatatcg cccagcaata acacaggctg gggcctgcgc ttcccaagca 15960 agatgtttgg cggggccaag aagcgctccg accaacaccc agtgcgcgtg cgcgggcact 16020 accgcgcgcc ctggggcgcg cacaaacgcg gccgcactgg gcgcaccacc gtcgatgacg 16080 ccatcgacgc ggtggtggag gaggcgcgca actacacgcc cacgccgcca ccagtgtcca 16140 cagtggacgc ggccattcag accgtggtgc gcggagcccg gcgctatgct aaaatgaaga 16200 gacggcggag gcgcgtagca cgtcgccacc gccgccgacc cggcactgcc gcccaacgcg 16260 cggcggcggc cctgcttaac cgcgcacgtc gcaccggccg acgggcggcc atgcgggccg 16320 ctcgaaggct ggccgcgggt attgtcactg tgccccccag gtccaggcga cgagcggccg 16380 ccgcagcagc cgcggccatt agtgctatga ctcagggtcg caggggcaac gtgtattggg 16440 tgcgcgactc ggttagcggc ctgcgcgtgc ccgtgcgcac ccgccccccg cgcaactaga 16500 ttgcaagaaa aaactactta gactcgtact gttgtatgta tccagcggcg gcggcgcgca 16560 acgaagctat gtccaagcgc aaaatcaaag aagagatgct ccaggtcatc gcgccggaga 16620 tctatggccc cccgaagaag gaagagcagg attacaagcc ccgaaagcta aagcgggtca 16680 aaaagaaaaa gaaagatgat gatgatgaac ttgacgacga ggtggaactg ctgcacgcta 16740 ccgcgcccag gcgacgggta cagtggaaag gtcgacgcgt aaaacgtgtt ttgcgacccg 16800 gcaccaccgt agtctttacg cccggtgagc gctccacccg cacctacaag cgcgtgtatg 16860 atgaggtgta cggcgacgag gacctgcttg agcaggccaa cgagcgcctc ggggagtttg 16920 cctacggaaa gcggcataag gacatgctgg cgttgccgct ggacgagggc aacccaacac 16980 ctagcctaaa gcccgtaaca ctgcagcagg tgctgcccgc gcttgcaccg tccgaagaaa 17040 agcgcggcct aaagcgcgag tctggtgact tggcacccac cgtgcagctg atggtaccca 17100 agcgccagcg actggaagat gtcttggaaa aaatgaccgt ggaacctggg ctggagcccg 17160 aggtccgcgt gcggccaatc aagcaggtgg cgccgggact gggcgtgcag accgtggacg 17220 ttcagatacc cactaccagt agcaccagta ttgccaccgc cacagagggc atggagacac 17280 aaacgtcccc ggttgcctca gcggtggcgg atgccgcggt gcaggcggtc gctgcggccg 17340 cgtccaagac ctctacggag gtgcaaacgg acccgtggat gtttcgcgtt tcagcccccc 17400 ggcgcccgcg cggttcgagg aagtacggcg ccgccagcgc gctactgccc gaatatgccc 17460 tacatccttc cattgcgcct acccccggct atcgtggcta cacctaccgc cccagaagac 17520 gagcaactac ccgacgccga accaccactg gaacccgccg ccgccgtcgc cgtcgccagc 17580 ccgtgctggc cccgatttcc gtgcgcaggg tggctcgcga aggaggcagg accctggtgc 17640 tgccaacagc gcgctaccac cccagcatcg tttaaaagcc ggtctttgtg gttcttgcag 17700 atatggccct cacctgccgc ctccgtttcc cggtgccggg attccgagga agaatgcacc 17760 gtaggagggg catggccggc cacggcctga cgggcggcat gcgtcgtgcg caccaccggc 17820 ggcggcgcgc gtcgcaccgt cgcatgcgcg gcggtatcct gcccctcctt attccactga 17880 tcgccgcggc gattggcgcc gtgcccggaa ttgcatccgt ggccttgcag gcgcagagac 17940 actgattaaa aacaagttgc atgtggaaaa atcaaaataa aaagtctgga ctctcacgct 18000 cgcttggtcc tgtaactatt ttgtagaatg gaagacatca actttgcgtc tctggccccg 18060 cgacacggct cgcgcccgtt catgggaaac tggcaagata tcggcaccag caatatgagc 18120 ggtggcgcct tcagctgggg ctcgctgtgg agcggcatta aaaatttcgg ttccaccgtt 18180 aagaactatg gcagcaaggc ctggaacagc agcacaggcc agatgctgag ggataagttg 18240 aaagagcaaa atttccaaca aaaggtggta gatggcctgg cctctggcat tagcggggtg 18300 gtggacctgg ccaaccaggc agtgcaaaat aagattaaca gtaagcttga tccccgccct 18360 cccgtagagg agcctccacc ggccgtggag acagtgtctc cagaggggcg tggcgaaaag 18420 cgtccgcgcc ccgacaggga agaaactctg gtgacgcaaa tagacgagcc tccctcgtac 18480 gaggaggcac taaagcaagg cctgcccacc acccgtccca tcgcgcccat ggctaccgga 18540 gtgctgggcc agcacacacc cgtaacgctg gacctgcctc cccccgccga cacccagcag 18600 aaacctgtgc tgccaggccc gaccgccgtt gttgtaaccc gtcctagccg cgcgtccctg 18660 cgccgcgccg ccagcggtcc gcgatcgttg cggcccgtag ccagtggcaa ctggcaaagc 18720 acactgaaca gcatcgtggg tctgggggtg caatccctga agcgccgacg atgcttctga 18780 atagctaacg tgtcgtatgt gtgtcatgta tgcgtccatg tcgccgccag aggagctgct 18840 gagccgccgc gcgcccgctt tccaagatgg ctaccccttc gatgatgccg cagtggtctt 18900 acatgcacat ctcgggccag gacgcctcgg agtacctgag ccccgggctg gtgcagtttg 18960 cccgcgccac cgagacgtac ttcagcctga ataacaagtt tagaaacccc acggtggcgc 19020 ctacgcacga cgtgaccaca gaccggtccc agcgtttgac gctgcggttc atccctgtgg 19080 accgtgagga tactgcgtac tcgtacaagg cgcggttcac cctagctgtg ggtgataacc 19140 gtgtgctgga catggcttcc acgtactttg acatccgcgg cgtgctggac aggggcccta 19200 cttttaagcc ctactctggc actgcctaca acgccctggc tcccaagggt gccccaaatc 19260 cttgcgaatg ggatgaagct gctactgctc ttgaaataaa cctagaagaa gaggacgatg 19320 acaacgaaga cgaagtagac gagcaagctg agcagcaaaa aactcacgta tttgggcagg 19380 cgccttattc tggtataaat attacaaagg agggtattca aataggtgtc gaaggtcaaa 19440 cacctaaata tgccgataaa acatttcaac ctgaacctca aataggagaa tctcagtggt 19500 acgaaactga aattaatcat gcagctggga gagtccttaa aaagactacc ccaatgaaac 19560 catgttacgg ttcatatgca aaacccacaa atgaaaatgg agggcaaggc attcttgtaa 19620 agcaacaaaa tggaaagcta gaaagtcaag tggaaatgca atttttctca actactgagg 19680 cgaccgcagg caatggtgat aacttgactc ctaaagtggt attgtacagt gaagatgtag 19740 atatagaaac cccagacact catatttctt acatgcccac tattaaggaa ggtaactcac 19800 gagaactaat gggccaacaa tctatgccca acaggcctaa ttacattgct tttagggaca 19860 attttattgg tctaatgtat tacaacagca cgggtaatat gggtgttctg gcgggccaag 19920 catcgcagtt gaatgctgtt gtagatttgc aagacagaaa cacagagctt tcataccagc 19980 ttttgcttga ttccattggt gatagaacca ggtacttttc tatgtggaat caggctgttg 20040 acagctatga tccagatgtt agaattattg aaaatcatgg aactgaagat gaacttccaa 20100 attactgctt tccactggga ggtgtgatta atacagagac tcttaccaag gtaaaaccta 20160 aaacaggtca ggaaaatgga tgggaaaaag atgctacaga attttcagat aaaaatgaaa 20220 taagagttgg aaataatttt gccatggaaa tcaatctaaa tgccaacctg tggagaaatt 20280 tcctgtactc caacatagcg ctgtatttgc ccgacaagct aaagtacagt ccttccaacg 20340 taaaaatttc tgataaccca aacacctacg actacatgaa caagcgagtg gtggctcccg 20400 ggttagtgga ctgctacatt aaccttggag cacgctggtc ccttgactat atggacaacg 20460 tcaacccatt taaccaccac cgcaatgctg gcctgcgcta ccgctcaatg ttgctgggca 20520 atggtcgcta tgtgcccttc cacatccagg tgcctcagaa gttctttgcc attaaaaacc 20580 tccttctcct gccgggctca tacacctacg agtggaactt caggaaggat gttaacatgg 20640 ttctgcagag ctccctagga aatgacctaa gggttgacgg agccagcatt aagtttgata 20700 gcatttgcct ttacgccacc ttcttcccca tggcccacaa caccgcctcc acgcttgagg 20760 ccatgcttag aaacgacacc aacgaccagt cctttaacga ctatctctcc gccgccaaca 20820 tgctctaccc tatacccgcc aacgctacca acgtgcccat atccatcccc tcccgcaact 20880 gggcggcttt ccgcggctgg gccttcacgc gccttaagac taaggaaacc ccatcactgg 20940 gctcgggcta cgacccttat tacacctact ctggctctat accctaccta gatggaacct 21000 tttacctcaa ccacaccttt aagaaggtgg ccattacctt tgactcttct gtcagctggc 21060 ctggcaatga ccgcctgctt acccccaacg agtttgaaat taagcgctca gttgacgggg 21120 agggttacaa cgttgcccag tgtaacatga ccaaagactg gttcctggta caaatgctag 21180 ctaactacaa cattggctac cagggcttct atatcccaga gagctacaag gaccgcatgt 21240 actccttctt tagaaacttc cagcccatga gccgtcaggt ggtggatgat actaaataca 21300 aggactacca acaggtgggc atcctacacc aacacaacaa ctctggattt gttggctacc 21360 ttgcccccac catgcgcgaa ggacaggcct accctgctaa cttcccctat ccgcttatag 21420 gcaagaccgc agttgacagc attacccaga aaaagtttct ttgcgatcgc accctttggc 21480 gcatcccatt ctccagtaac tttatgtcca tgggcgcact cacagacctg ggccaaaacc 21540 ttctctacgc caactccgcc cacgcgctag acatgacttt tgaggtggat cccatggacg 21600 agcccaccct tctttatgtt ttgtttgaag tctttgacgt ggtccgtgtg caccggccgc 21660 accgcggcgt catcgaaacc gtgtacctgc gcacgccctt ctcggccggc aacgccacaa 21720 cataaagaag caagcaacat caacaacagc tgccgccatg ggctccagtg agcaggaact 21780 gaaagccatt gtcaaagatc ttggttgtgg gccatatttt ttgggcacct atgacaagcg 21840 ctttccaggc tttgtttctc cacacaagct cgcctgcgcc atagtcaata cggccggtcg 21900 cgagactggg ggcgtacact ggatggcctt tgcctggaac ccgcactcaa aaacatgcta 21960 cctctttgag ccctttggct tttctgacca gcgactcaag caggtttacc agtttgagta 22020 cgagtcactc ctgcgccgta gcgccattgc ttcttccccc gaccgctgta taacgctgga 22080 aaagtccacc caaagcgtac aggggcccaa ctcggccgcc tgtggactat tctgctgcat 22140 gtttctccac gcctttgcca actggcccca aactcccatg gatcacaacc ccaccatgaa 22200 ccttattacc ggggtaccca actccatgct caacagtccc caggtacagc ccaccctgcg 22260 tcgcaaccag gaacagctct acagcttcct ggagcgccac tcgccctact tccgcagcca 22320 cagtgcgcag attaggagcg ccacttcttt ttgtcacttg aaaaacatgt aaaaataatg 22380 tactagagac actttcaata aaggcaaatg cttttatttg tacactctcg ggtgattatt 22440 tacccccacc cttgccgtct gcgccgttta aaaatcaaag gggttctgcc gcgcatcgct 22500 atgcgccact ggcagggaca cgttgcgata ctggtgttta gtgctccact taaactcagg 22560 cacaaccatc cgcggcagct cggtgaagtt ttcactccac aggctgcgca ccatcaccaa 22620 cgcgtttagc aggtcgggcg ccgatatctt gaagtcgcag ttggggcctc cgccctgcgc 22680 gcgcgagttg cgatacacag ggttgcagca ctggaacact atcagcgccg ggtggtgcac 22740 gctggccagc acgctcttgt cggagatcag atccgcgtcc aggtcctccg cgttgctcag 22800 ggcgaacgga gtcaactttg gtagctgcct tcccaaaaag ggcgcgtgcc caggctttga 22860 gttgcactcg caccgtagtg gcatcaaaag gtgaccgtgc ccggtctggg cgttaggata 22920 cagcgcctgc ataaaagcct tgatctgctt aaaagccacc tgagcctttg cgccttcaga 22980 gaagaacatg ccgcaagact tgccggaaaa ctgattggcc ggacaggccg cgtcgtgcac 23040 gcagcacctt gcgtcggtgt tggagatctg caccacattt cggccccacc ggttcttcac 23100 gatcttggcc ttgctagact gctccttcag cgcgcgctgc ccgttttcgc tcgtcacatc 23160 catttcaatc acgtgctcct tatttatcat aatgcttccg tgtagacact taagctcgcc 23220 ttcgatctca gcgcagcggt gcagccacaa cgcgcagccc gtgggctcgt gatgcttgta 23280 ggtcacctct gcaaacgact gcaggtacgc ctgcaggaat cgccccatca tcgtcacaaa 23340 ggtcttgttg ctggtgaagg tcagctgcaa cccgcggtgc tcctcgttca gccaggtctt 23400 gcatacggcc gccagagctt ccacttggtc aggcagtagt ttgaagttcg cctttagatc 23460 gttatccacg tggtacttgt ccatcagcgc gcgcgcagcc tccatgccct tctcccacgc 23520 agacacgatc ggcacactca gcgggttcat caccgtaatt tcactttccg cttcgctggg 23580 ctcttcctct tcctcttgcg tccgcatacc acgcgccact gggtcgtctt cattcagccg 23640 ccgcactgtg cgcttacctc ctttgccatg cttgattagc accggtgggt tgctgaaacc 23700 caccatttgt agcgccacat cttctctttc ttcctcgctg tccacgatta cctctggtga 23760 tggcgggcgc tcgggcttgg gagaagggcg cttctttttc ttcttgggcg caatggccaa 23820 atccgccgcc gaggtcgatg gccgcgggct gggtgtgcgc ggcaccagcg cgtcttgtga 23880 tgagtcttcc tcgtcctcgg actcgatacg ccgcctcatc cgcttttttg ggggcgcccg 23940 gggaggcggc ggcgacgggg acggggacga cacgtcctcc atggttgggg gacgtcgcgc 24000 cgcaccgcgt ccgcgctcgg gggtggtttc gcgctgctcc tcttcccgac tggccatttc 24060 cttctcctat aggcagaaaa agatcatgga gtcagtcgag aagaaggaca gcctaaccgc 24120 cccctctgag ttcgccacca ccgcctccac cgatgccgcc aacgcgccta ccaccttccc 24180 cgtcgaggca cccccgcttg aggaggagga agtgattatc gagcaggacc caggttttgt 24240 aagcgaagac gacgaggacc gctcagtacc aacagaggat aaaaagcaag accaggacaa 24300 cgcagaggca aacgaggaac aagtcgggcg gggggacgaa aggcatggcg actacctaga 24360 tgtgggagac gacgtgctgt tgaagcatct gcagcgccag tgcgccatta tctgcgacgc 24420 gttgcaagag cgcagcgatg tgcccctcgc catagcggat gtcagccttg cctacgaacg 24480 ccacctattc tcaccgcgcg taccccccaa acgccaagaa aacggcacat gcgagcccaa 24540 cccgcgcctc aacttctacc ccgtatttgc cgtgccagag gtgcttgcca cctatcacat 24600 ctttttccaa aactgcaaga tacccctatc ctgccgtgcc aaccgcagcc gagcggacaa 24660 gcagctggcc ttgcggcagg gcgctgtcat acctgatatc gcctcgctca acgaagtgcc 24720 aaaaatcttt gagggtcttg gacgcgacga gaagcgcgcg gcaaacgctc tgcaacagga 24780 aaacagcgaa aatgaaagtc actctggagt gttggtggaa ctcgagggtg acaacgcgcg 24840 cctagccgta ctaaaacgca gcatcgaggt cacccacttt gcctacccgg cacttaacct 24900 accccccaag gtcatgagca cagtcatgag tgagctgatc gtgcgccgtg cgcagcccct 24960 ggagagggat gcaaatttgc aagaacaaac agaggagggc ctacccgcag ttggcgacga 25020 gcagctagcg cgctggcttc aaacgcgcga gcctgccgac ttggaggagc gacgcaaact 25080 aatgatggcc gcagtgctcg ttaccgtgga gcttgagtgc atgcagcggt tctttgctga 25140 cccggagatg cagcgcaagc tagaggaaac attgcactac acctttcgac agggctacgt 25200 acgccaggcc tgcaagatct ccaacgtgga gctctgcaac ctggtctcct accttggaat 25260 tttgcacgaa aaccgccttg ggcaaaacgt gcttcattcc acgctcaagg gcgaggcgcg 25320 ccgcgactac gtccgcgact gcgtttactt atttctatgc tacacctggc agacggccat 25380 gggcgtttgg cagcagtgct tggaggagtg caacctcaag gagctgcaga aactgctaaa 25440 gcaaaacttg aaggacctat ggacggcctt caacgagcgc tccgtggccg cgcacctggc 25500 ggacatcatt ttccccgaac gcctgcttaa aaccctgcaa cagggtctgc cagacttcac 25560 cagtcaaagc atgttgcaga actttaggaa ctttatccta gagcgctcag gaatcttgcc 25620 cgccacctgc tgtgcacttc ctagcgactt tgtgcccatt aagtaccgcg aatgccctcc 25680 gccgctttgg ggccactgct accttctgca gctagccaac taccttgcct accactctga 25740 cataatggaa gacgtgagcg gtgacggtct actggagtgt cactgtcgct gcaacctatg 25800 caccccgcac cgctccctgg tttgcaattc gcagctgctt aacgaaagtc aaattatcgg 25860 tacctttgag ctgcagggtc cctcgcctga cgaaaagtcc gcggctccgg ggttgaaact 25920 cactccgggg ctgtggacgt cggcttacct tcgcaaattt gtacctgagg actaccacgc 25980 ccacgagatt aggttctacg aagaccaatc ccgcccgcca aatgcggagc ttaccgcctg 26040 cgtcattacc cagggccaca ttcttggcca attgcaagcc atcaacaaag cccgccaaga 26100 gtttctgcta cgaaagggac ggggggttta cttggacccc cagtccggcg aggagctcaa 26160 cccaatcccc ccgccgccgc agccctatca gcagcagccg cgggcccttg cttcccagga 26220 tggcacccaa aaagaagctg cagctgccgc cgccacccac ggacgaggag gaatactggg 26280 acagtcaggc agaggaggtt ttggacgagg aggaggagga catgatggaa gactgggaga 26340 gcctagacga ggaagcttcc gaggtcgaag aggtgtcaga cgaaacaccg tcaccctcgg 26400 tcgcattccc ctcgccggcg ccccagaaat cggcaaccgg ttccagcatg gctacaacct 26460 ccgctcctca ggcgccgccg gcactgcccg ttcgccgacc caaccgtaga tgggacacca 26520 ctggaaccag ggccggtaag tccaagcagc cgccgccgtt agcccaagag caacaacagc 26580 gccaaggcta ccgctcatgg cgcgggcaca agaacgccat agttgcttgc ttgcaagact 26640 gtgggggcaa catctccttc gcccgccgct ttcttctcta ccatcacggc gtggccttcc 26700 cccgtaacat cctgcattac taccgtcatc tctacagccc atactgcacc ggcggcagcg 26760 gcagcggcag caacagcagc ggccacacag aagcaaaggc gaccggatag caagactctg 26820 acaaagccca agaaatccac agcggcggca gcagcaggag gaggagcgct gcgtctggcg 26880 cccaacgaac ccgtatcgac ccgcgagctt agaaacagga tttttcccac tctgtatgct 26940 atatttcaac agagcagggg ccaagaacaa gagctgaaaa taaaaaacag gtctctgcga 27000 tccctcaccc gcagctgcct gtatcacaaa agcgaagatc agcttcggcg cacgctggaa 27060 gacgcggagg ctctcttcag taaatactgc gcgctgactc ttaaggacta gtttcgcgcc 27120 ctttctcaaa tttaagcgcg aaaactacgt catctccagc ggccacaccc ggcgccagca 27180 cctgtcgtca gcgccatttc aactttgtat acaaaagttg tgatgagcaa ggaaattccc 27240 acgccctaca tgtggagtta ccagccacaa atgggacttg cggctggagc tgcccaagac 27300 tactcaaccc gaataaacta catgagcgcg ggaccccaca tgatatcccg ggtcaacgga 27360 atacgcgccc accgaaaccg aattctcctg gaacaggcgg ctattaccac cacacctcgt 27420 aataacctta atccccgtag ttggcccgct gccctggtgt accaggaaag tcccgctccc 27480 accactgtgg tacttcccag agacgcccag gccgaagttc agatgactaa ctcaggggcg 27540 cagcttgcgg gcggctttcg tcacagggtg cggtcgcccg ggcagggtat aactcacctg 27600 acaatcagag ggcgaggtat tcagctcaac gacgagtcgg tgagctcctc gcttggtctc 27660 cgtccggacg ggacatttca gatcggcggc gccggccgct cttcattcac gcctcgtcag 27720 gcaatcctaa ctctgcagac ctcgtcctct gagccgcgct ctggaggcat tggaactctg 27780 caatttattg aggagtttgt gccatcggtc tactttaacc ccttctcggg acctcccggc 27840 cactatccgg atcaatttat tcctaacttt gacgcggtaa aggactcggc ggacggctac 27900 gactgaatgt taagtggaga ggcagagcaa ctgcgcctga aacacctggt ccactgtcgc 27960 cgccacaagt gctttgcccg cgactccggt gagttttgct actttgaatt gcccgaggat 28020 catatcgagg gcccggcgca cggcgtccgg cttaccgccc agggagagct tgcccgtagc 28080 ctgattcggg agtttaccca gcgccccctg ctagttgagc gggacagggg accctgtgtt 28140 ctcactgtga tttgcaactg tcctaaccct ggattacatc aagatctttg ttgccatctc 28200 tgtgctgagt ataataaata cagaaattaa aatatactgg ggctcctatc gccatcctgt 28260 aaacgccacc gtcttcaccc gcccaagcaa accaaggcga accttacctg gtacttttaa 28320 catctctccc tctgtgattt acaacagttt caacccagac ggagtgagtc tacgagagaa 28380 cctctccgag ctcagctact ccatcagaaa aaacaccacc ctccttacct gccgggaacg 28440 tacgagtgcg tcaccggccg ctgcaccaca cctaccgcct gaccgtaaac cagacttttt 28500 ccggacagac ctcaataact ctgtttacca gaacaggagg tgagcttaga aaacccttag 28560 ggtattaggc caaaggcgca gctactgtgg ggtttatgaa caattcaagc aactctacgg 28620 gctattctaa ttcaggtttc tctagaatcg gggttggggt tattctctgt cttgtgattc 28680 tctttattct tatactaacg cttctctgcc taaggctcgc cgcctgctgt gtgcacattt 28740 gcatttattg tcagcttttt aaacgctggg gtcgccaccc aagatgatta ggtacataat 28800 cctaggttta ctcacccttg cgtcagccca cggtaccacc caaaaggtgg attttaagga 28860 gccagcctgt aatgttacat tcgcagctga agctaatgag tgcaccactc ttataaaatg 28920 caccacagaa catgaaaagc tgcttattcg ccacaaaaac aaaattggca agtatgctgt 28980 ttatgctatt tggcagccag gtgacactac agagtataat gttacagttt tccagggtaa 29040 aagtcataaa acttttatgt atacttttcc attttatgaa atgtgcgaca ttaccatgta 29100 catgagcaaa cagtataagt tgtggccccc acaaaattgt gtggaaaaca ctggcacttt 29160 ctgctgcact gctatgctaa ttacagtgct cgctttggtc tgtaccctac tctatattaa 29220 atacaaaagc agacgcagct ttattgagga aaagaaaatg ccttaattta ctaagttaca 29280 aagctaatgt caccactaac tgctttactc gctgcttgca aaacaaattc aaaaagttag 29340 cattataatt agaataggat ttaaaccccc cggtcatttc ctgctcaata ccattcccct 29400 gaacaattga ctctatgtgg gatatgctcc agcgctacaa ccttgaagtc aggcttcctg 29460 gatgtcagca tctgactttg gccagcacct gtcccgcgga tttgttccag tccaactaca 29520 gcgacccacc ctaacagaga tgaccaacac aaccaacgcg gccgccgcta ccggacttac 29580 atctaccaca aatacacccc aagtttctgc ctttgtcaat aactgggata acttgggcat 29640 gtggtggttc tccatagcgc ttatgtttgt atgccttatt attatgtggc tcatctgctg 29700 cctaaagcgc aaacgcgccc gaccacccat ctatagtccc atcattgtgc tacacccaaa 29760 caatgatgga atccatagat tggacggact gaaacacatg ttcttttctc ttacagtatg 29820 attaaatgag acatgattcc tcgagttttt atattactga cccttgttgc gcttttttgt 29880 gcgtgctcca cattggctgc ggtttctcac atcgaagtag actgcattcc agccttcaca 29940 gtctatttgc tttacggatt tgtcaccctc acgctcatct gcagcctcat cactgtggtc 30000 atcgccttta tccagtgcat tgactgggtc tgtgtgcgct ttgcatatct cagacaccat 30060 ccccagtaca gggacaggac tatagctgag cttcttagaa ttctttaatt atgaaattta 30120 ctgtgacttt tctgctgatt atttgcaccc tatctgcgtt ttgttccccg acctccaagc 30180 ctcaaagaca tatatcatgc agattcactc gtatatggaa tattccaagt tgctacaatg 30240 aaaaaagcga tctttccgaa gcctggttat atgcaatcat ctctgttatg gtgttctgca 30300 gtaccatctt agccctagct atatatccct accttgacat tggctggaac gcaatagatg 30360 ccatgaacca cccaactttc cccgcgcccg ctatgcttcc actgcaacaa gttgttgccg 30420 gcggctttgt cccagccaat cagcctcgcc caccttctcc cacccccact gaaatcagct 30480 actttaatct aacaggagga gatgactgac accctagatc tagaaatgga cggaattatt 30540 acagagcagc gcctgctaga aagacgcagg gcagcggccg agcaacagcg catgaatcaa 30600 gagctccaag acatggttaa cttgcaccag tgcaaaaggg gtatcttttg tctggtaaag 30660 caggccaaag tcacctacga cagtaatacc accggacacc gccttagcta caagttgcca 30720 accaagcgtc agaaattggt ggtcatggtg ggagaaaagc ccattaccat aactcagcac 30780 tcggtagaaa ccgaaggctg cattcactca ccttgtcaag gacctgagga tctctgcacc 30840 cttattaaga ccctgtgcgg tctcaaagat cttattccct ttaactaata aaaaaaaata 30900 ataaagcatc acttacttaa aatcagttag caaatttctg tccagtttat tcagcagcac 30960 ctccttgccc tcctcccagc tctggtattg cagcttcctc ctggctgcaa actttctcca 31020 caatctaaat ggaatgtcag tttcctcctg ttcctgtcca tccgcaccca ctatcttcat 31080 gttgttgcag atgaagcgcg caagaccgtc tgaagatacc ttcaaccccg tgtatccata 31140 tgacacggaa accggtcctc caactgtgcc ttttcttact cctccctttg tatcccccaa 31200 tgggtttcaa gagagtcccc ctggggtact ctctttgcgc ctatccgaac ctctagttac 31260 ctccaatggc atgcttgcgc tcaaaatggg caacggcctc tctctggacg aggccggcaa 31320 ccttacctcc caaaatgtaa ccactgtgag cccacctctc aaaaaaacca agtcaaacat 31380 aaacctggaa atatctgcac ccctcacagt tacctcagaa gccctaactg tggctgccgc 31440 cgcacctcta atggtcgcgg gcaacacact caccatgcaa tcacaggccc cgctaaccgt 31500 gcacgactcc aaacttagca ttgccaccca aggacccctc acagtgtcag aaggaaagct 31560 agccctgcaa acatcaggcc ccctcaccac caccgatagc agtaccctta ctatcactgc 31620 ctcaccccct ctaactactg ccactggtag cttgggcatt gacttgaaag agcccattta 31680 tacacaaaat ggaaaactag gactaaagta cggggctcct ttgcatgtaa cagacgacct 31740 aaacactttg accgtagcaa ctggtccagg tgtgactatt aataatactt ccttgcaaac 31800 taaagttact ggagccttgg gttttgattc acaaggcaat atgcaactta atgtagcagg 31860 aggactaagg attgattctc aaaacagacg ccttatactt gatgttagtt atccgtttga 31920 tgctcaaaac caactaaatc taagactagg acagggccct ctttttataa actcagccca 31980 caacttggat attaactaca acaaaggcct ttacttgttt acagcttcaa acaattccaa 32040 aaagcttgag gttaacctaa gcactgccaa ggggttgatg tttgacgcta cagccatagc 32100 cattaatgca ggagatgggc ttgaatttgg ttcacctaat gcaccaaaca caaatcccct 32160 caaaacaaaa attggccatg gcctagaatt tgattcaaac aaggctatgg ttcctaaact 32220 aggaactggc cttagttttg acagcacagg tgccattaca gtaggaaaca aaaataatga 32280 taagctaact ttgtggacca caccagctcc atctcctaac tgtagactaa atgcagagaa 32340 agatgctaaa ctcactttgg tcttaacaaa atgtggcagt caaatacttg ctacagtttc 32400 agttttggct gttaaaggca gtttggctcc aatatctgga acagttcaaa gtgctcatct 32460 tattataaga tttgacgaaa atggagtgct actaaacaat tccttcctgg acccagaata 32520 ttggaacttt agaaatggag atcttactga aggcacagcc tatacaaacg ctgttggatt 32580 tatgcctaac ctatcagctt atccaaaatc tcacggtaaa actgccaaaa gtaacattgt 32640 cagtcaagtt tacttaaacg gagacaaaac taaacctgta acactaacca ttacactaaa 32700 cggtacacag gaaacaggag acacaactcc aagtgcatac tctatgtcat tttcatggga 32760 ctggtctggc cacaactaca ttaatgaaat atttgccaca tcctcttaca ctttttcata 32820 cattgcccaa gaataaagaa tcgtttgtgt tatgtttcaa cgtgtttatt tttcaattgc 32880 agaaaatttc aagtcatttt tcattcagta gtatagcccc accaccacat agcttataca 32940 gatcaccgta cctcaacttt gtataataaa gttgaatcaa actcacagaa ccctagtatt 33000 caacctgcca cctccctccc aacacacaga gtacacagtc ctttctcccc ggctggcctt 33060 aaaaagcatc atatcatggg taacagacat attcttaggt gttatattcc acacggtttc 33120 ctgtcgagcc aaacgctcat cagtgatatt aataaactcc ccgggcagct cacttaagtt 33180 catgtcgctg tccagctgct gagccacagg ctgctgtcca acttgcggtt gcttaacggg 33240 cggcgaagga gaagtccacg cctacatggg ggtagagtca taatcgtgca tcaggatagg 33300 gcggtggtgc tgcagcagcg cgcgaataaa ctgctgccgc cgccgctccg tcctgcagga 33360 atacaacatg gcagtggtct cctcagcgat gattcgcacc gcccgcagca taaggcgcct 33420 tgtcctccgg gcacagcagc gcaccctgat ctcacttaaa tcagcacagt aactgcagca 33480 cagcaccaca atattgttca aaatcccaca gtgcaaggcg ctgtatccaa agctcatggc 33540 ggggaccaca gaacccacgt ggccatcata ccacaagcgc aggtagatta agtggcgacc 33600 cctcataaac acgctggaca taaacattac ctcttttggc atgttgtaat tcaccacctc 33660 ccggtaccat ataaacctct gattaaacat ggcgccatcc accaccatcc taaaccagct 33720 ggccaaaacc tgcccgccgg ctatacactg cagggaaccg ggactggaac aatgacagtg 33780 gagagcccag gactcgtaac catggatcat catgctcgtc atgatatcaa tgttggcaca 33840 acacaggcac acgtgcatac acttcctcag gattacaagc tcctcccgcg ttagaaccat 33900 atcccaggga acaacccatt cctgaatcag cgtaaatccc acactgcagg gaagacctcg 33960 cacgtaactc acgttgtgca ttgtcaaagt gttacattcg ggcagcagcg gatgatcctc 34020 cagtatggta gcgcgggttt ctgtctcaaa aggaggtaga cgatccctac tgtacggagt 34080 gcgccgagac aaccgagatc gtgttggtcg tagtgtcatg ccaaatggaa cgccggacgt 34140 agtcatattt cctgaagcaa aaccaggtgc gggcgtgaca aacagatctg cgtctccggt 34200 ctcgccgctt agatcgctct gtgtagtagt tgtagtatat ccactctctc aaagcatcca 34260 ggcgccccct ggcttcgggt tctatgtaaa ctccttcatg cgccgctgcc ctgataacat 34320 ccaccaccgc agaataagcc acacccagcc aacctacaca ttcgttctgc gagtcacaca 34380 cgggaggagc gggaagagct ggaagaacca tgtttttttt tttattccaa aagattatcc 34440 aaaacctcaa aatgaagatc tattaagtga acgcgctccc ctccggtggc gtggtcaaac 34500 tctacagcca aagaacagat aatggcattt gtaagatgtt gcacaatggc ttccaaaagg 34560 caaacggccc tcacgtccaa gtggacgtaa aggctaaacc cttcagggtg aatctcctct 34620 ataaacattc cagcaccttc aaccatgccc aaataattct catctcgcca ccttctcaat 34680 atatctctaa gcaaatcccg aatattaagt ccggccattg taaaaatctg ctccagagcg 34740 ccctccacct tcagcctcaa gcagcgaatc atgattgcaa aaattcaggt tcctcacaga 34800 cctgtataag attcaaaagc ggaacattaa caaaaatacc gcgatcccgt aggtcccttc 34860 gcagggccag ctgaacataa tcgtgcaggt ctgcacggac cagcgcggcc acttccccgc 34920 caggaaccat gacaaaagaa cccacactga ttatgacacg catactcgga gctatgctaa 34980 ccagcgtagc cccgatgtaa gcttgttgca tgggcggcga tataaaatgc aaggtgctgc 35040 tcaaaaaatc aggcaaagcc tcgcgcaaaa aagaaagcac atcgtagtca tgctcatgca 35100 gataaaggca ggtaagctcc ggaaccacca cagaaaaaga caccattttt ctctcaaaca 35160 ttgggccagg attctcctcg acgtcaccag cctgcttcag caggctgaag ttagtagctc 35220 cgcttccctt atagagctcg ttcatgccct cggtgatgcc ggcggcggtc aggaactcca 35280 gcagcaccat gtggtcccgc ttctcgttgg ggtccttgaa cagggcgctc tggtagctca 35340 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt 35400 ggtcggccag ctgcacgccg ccgtcctcga tgttgtgccg gatcttgaag ttggccttga 35460 tgccgttctt ctgcttgtcg gcggtgatgt acacgttgtg gctgttgtag ttgtactcca 35520 gcttgtggcc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatccggt 35580 tcaccagggt gtcgccctcg aacttcacct cggcccgggt cttgtagttg ccgtcgtcct 35640 tgaagaagat ggtccgctcc tgcacgtagc cctcgggcat ggcgctcttg aagaagtcgt 35700 gctgcttcat gtggtcgggg taccgggcga agcactgcac gccgtagccc agggtggtca 35760 ccagggtggg ccagggcacg ggcagcttgc cggtggtgca cagcagcttc agggtcagct 35820 tgccgtaggt ggcgtcgccc tcgccctcgc cgctcacgct gaacttgtgg ccgttcacgt 35880 cgccgtccag ctccaccagg atgggcacca cgccggtgaa cagctcttcg cctttgctca 35940 ccatgtctgc gggtttctgc ataaacacaa aataaaataa caaaaaaaca tttaaacatt 36000 agaagcctgt cttacaacag gaaaaacaac ccttataagc ataagacgga ctacggccat 36060 gccggcgtga ccgtaaaaaa actggtcacc gtgattaaaa agcaccaccg acagctcctc 36120 ggtcatgtcc ggagtcataa tgtaagactc ggtaaacaca tcaggttgat tcacatcggt 36180 cagtgctaaa aagcgaccga aatagcccgg gggaatacat acccgcaggc gtagagacaa 36240 cattacagcc cccataggag gtataacaaa attaatagga gagaaaaaca cataaacacc 36300 tgaaaaaccc tcctgcctag gcaaaatagc accctcccgc tccagaacaa catacagcgc 36360 ttccacagcg gcagccataa cagtcagcct taccagtaaa aaagaaaacc tattaaaaaa 36420 acaccactcg acacggcacc agctcaatca gtcacagtgt aaaaaagggc caagtgcaga 36480 gcgagtatat ataggactaa aaaatgacgt aacggttaaa gtccacaaaa aacacccaga 36540 aaaccgcacg cgaacctacg cccagaaacg aaagccaaaa aacccacaac ttcctcaaat 36600 cgtcacttcc gttttcccac gttacgtcac ttcccatttt aagaaaacta caattcccaa 36660 cacatacaag ttactccgcc ctaaaaccta cgtcacccgc cccgttccca cgccccgcgc 36720 cacgtcacaa actccacccc ctcattatca tattggcttc aatccaaaat aaggtatatt 36780 attgatgatg 36790 <210> 11 <211> 36781 <212> DNA <213> Artificial Sequence <220> <223> Synthetic adenovirus genome mCherry-P2A-E4-ORF2 <400> 11 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420 cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480 tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540 tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600 aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660 tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720 cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780 gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840 cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900 ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960 cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020 caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080 ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140 tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200 gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260 ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320 cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380 ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440 gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500 cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560 ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620 gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680 cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740 ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800 tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860 gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920 caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980 gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040 agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100 aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160 cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220 gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280 gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340 gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400 gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460 tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520 ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580 tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640 agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700 tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760 gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820 acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880 gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940 aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000 ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060 gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120 tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180 acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240 aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300 tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360 gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420 tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480 ttggctctag cgatgaagat acagattgag gtactgaaat gtgtgggcgt ggcttaaggg 3540 tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg 3600 ccgccgccca acttttctat acaaagttgt aatgagcacc aactcgtttg atggaagcat 3660 tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat 3720 gggctccagc attgatggtc gccccgtcct gcccgcaaac tctactacct tgacctacga 3780 gaccgtgtct ggaacgccgt tggagactgc agcctccgcc gccgcttcag ccgctgcagc 3840 caccgcccgc gggattgtga ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc 3900 ttcccgttca tccgcccgcg atgacaagtt gacggctctt ttggcacaat tggattcttt 3960 gacccgggaa cttaatgtcg tttctcagca gctgttggat ctgcgccagc aggtttctgc 4020 cctgaaggct tcctcccctc ccaatgcggt ttaaaacata aataaaaaac cagactctgt 4080 ttggatttgg atcaagcata agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg 4140 taggcccggg accagcggtc tcggtcgttg agggtcctgt gtattttttc caggacgtgg 4200 taaaggtgac tctggatgtt cagatacatg ggcataagcc cgtctctggg gtggaggtag 4260 caccactgca gagcttcatg ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag 4320 cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc tgattgccag gggcaggccc 4380 ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt gcatacgtgg ggatatgaga 4440 tgcatcttgg actgtatttt taggttggct atgttcccag ccatatccct ccggggattc 4500 atgttgtgca gaaccaccag cacagtgtat ccggtgcact tgggaaattt gtcatgtagc 4560 ttagaaggaa atgcgtggaa gaacttggag acgcccttgt gacctccaag attttccatg 4620 cattcgtcca taatgatggc aatgggccca cgggcggcgg cctgggcgaa gatatttctg 4680 ggatcactaa cgtcatagtt gtgttccagg atgagatcgt cataggccat ttttacaaag 4740 cgcgggcgga gggtgccaga ctgcggtata atggttccat ccggcccagg ggcgtagtta 4800 ccctcacaga tttgcatttc ccacgctttg agttcagatg gggggatcat gtctacctgc 4860 ggggcgatga agaaaacggt ttccggggta ggggagatca gctgggaaga aagcaggttc 4920 ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa tcacacctat taccgggtgc 4980 aactggtagt taagagagct gcagctgccg tcatccctga gcaggggggc cacttcgtta 5040 agcatgtccc tgactcgcat gttttccctg accaaatccg ccagaaggcg ctcgccgccc 5100 agcgatagca gttcttgcaa ggaagcaaag tttttcaacg gtttgagacc gtccgccgta 5160 ggcatgcttt tgagcgtttg accaagcagt tccaggcggt cccacagctc ggtcacctgc 5220 tctacggcat ctcgatccag catatctcct cgtttcgcgg gttggggcgg ctttcgctgt 5280 acggcagtag tcggtgctcg tccagacggg ccagggtcat gtctttccac gggcgcaggg 5340 tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc tccgggctgc gcgctggcca 5400 gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt 5460 cggccaggta gcatttgacc atggtgtcat agtccagccc ctccgcggcg tggcccttgg 5520 cgcgcagctt gcccttggag gaggcgccgc acgaggggca gtgcagactt ttgagggcgt 5580 agagcttggg cgcgagaaat accgattccg gggagtaggc atccgcgccg caggccccgc 5640 agacggtctc gcattccacg agccaggtga gctctggccg ttcggggtca aaaaccaggt 5700 ttcccccatg ctttttgatg cgtttcttac ctctggtttc catgagccgg tgtccacgct 5760 cggtgacgaa aaggctgtcc gtgtccccgt atacagactt gagaggcctg tcctcgagcg 5820 gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacaaag gctcgcgtcc 5880 aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact agggggtcca 5940 ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag gtgattggtt 6000 tgtaggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag ggggtggggg 6060 cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt tggggtgagt 6120 actccctctg aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg 6180 aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgca tccatctggt 6240 cagaaaagac aatctttttg ttgtcaagct tggtggcaaa cgacccgtag agggcgttgg 6300 acagcaactt ggcgatggag cgcagggttt ggtttttgtc gcgatcggcg cgctccttgg 6360 ccgcgatgtt tagctgcacg tattcgcgcg caacgcaccg ccattcggga aagacggtgg 6420 tgcgctcgtc gggcaccagg tgcacgcgcc aaccgcggtt gtgcagggtg acaaggtcaa 6480 cgctggtggc tacctctccg cgtaggcgct cgttggtcca gcagaggcgg ccgcccttgc 6540 gcgagcagaa tggcggtagg gggtctagct gcgtctcgtc cggggggtct gcgtccacgg 6600 taaagacccc gggcagcagg cgcgcgtcga agtagtctat cttgcatcct tgcaagtcta 6660 gcgcctgctg ccatgcgcgg gcggcaagcg cgcgctcgta tgggttgagt gggggacccc 6720 atggcatggg gtgggtgagc gcggaggcgt acatgccgca aatgtcgtaa acgtagaggg 6780 gctctctgag tattccaaga tatgtagggt agcatcttcc accgcggatg ctggcgcgca 6840 cgtaatcgta tagttcgtgc gagggagcga ggaggtcggg accgaggttg ctacgggcgg 6900 gctgctctgc tcggaagact atctgcctga agatggcatg tgagttggat gatatggttg 6960 gacgctggaa gacgttgaag ctggcgtctg tgagacctac cgcgtcacgc acgaaggagg 7020 cgtaggagtc gcgcagcttg ttgaccagct cggcggtgac ctgcacgtct agggcgcagt 7080 agtccagggt ttccttgatg atgtcatact tatcctgtcc cttttttttc cacagctcgc 7140 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct 7200 ccgaacggta agagcctagc atgtagaact ggttgacggc ctggtaggcg cagcatccct 7260 tttctacggg tagcgcgtat gcctgcgcgg ccttccggag cgaggtgtgg gtgagcgcaa 7320 aggtgtccct gaccatgact ttgaggtact ggtatttgaa gtcagtgtcg tcgcatccgc 7380 cctgctccca gagcaaaaag tccgtgcgct ttttggaacg cggatttggc agggcgaagg 7440 tgacatcgtt gaagagtatc tttcccgcgc gaggcataaa gttgcgtgtg atgcggaagg 7500 gtcccggcac ctcggaacgg ttgttaatta cctgggcggc gagcacgatc tcgtcaaagc 7560 cgttgatgtt gtggcccaca atgtaaagtt ccaagaagcg cgggatgccc ttgatggaag 7620 gcaatttttt aagttcctcg taggtgagct cttcagggga gctgagcccg tgctctgaaa 7680 gggcccagtc tgcaagatga gggttggaag cgacgaatga gctccacagg tcacgggcca 7740 ttagcatttg caggtggtcg cgaaaggtcc taaactggcg acctatggcc attttttctg 7800 gggtgatgca gtagaaggta agcgggtctt gttcccagcg gtcccatcca aggttcgcgg 7860 ctaggtctcg cgcggcagtc actagaggct catctccgcc gaacttcatg accagcatga 7920 agggcacgag ctgcttccca aaggccccca tccaagtata ggtctctaca tcgtaggtga 7980 caaagagacg ctcggtgcga ggatgcgagc cgatcgggaa gaactggatc tcccgccacc 8040 aattggagga gtggctattg atgtggtgaa agtagaagtc cctgcgacgg gccgaacact 8100 cgtgctggct tttgtaaaaa cgtgcgcagt actggcagcg gtgcacgggc tgtacatcct 8160 gcacgaggtt gacctgacga ccgcgcacaa ggaagcagag tgggaatttg agcccctcgc 8220 ctggcgggtt tggctggtgg tcttctactt cggctgcttg tccttgaccg tctggctgct 8280 cgaggggagt tacggtggat cggaccacca cgccgcgcga gcccaaagtc cagatgtccg 8340 cgcgcggcgg tcggagcttg atgacaacat cgcgcagatg ggagctgtcc atggtctgga 8400 gctcccgcgg cgtcaggtca ggcgggagct cctgcaggtt tacctcgcat agacgggtca 8460 gggcgcgggc tagatccagg tgatacctaa tttccagggg ctggttggtg gcggcgtcga 8520 tggcttgcaa gaggccgcat ccccgcggcg cgactacggt accgcgcggc gggcggtggg 8580 ccgcgggggt gtccttggat gatgcatcta aaagcggtga cgcgggcgag cccccggagg 8640 tagggggggc tccggacccg ccgggagagg gggcaggggc acgtcggcgc cgcgcgcggg 8700 caggagctgg tgctgcgcgc gtaggttgct ggcgaacgcg acgacgcggc ggttgatctc 8760 ctgaatctgg cgcctctgcg tgaagacgac gggcccggtg agcttgagcc tgaaagagag 8820 ttcgacagaa tcaatttcgg tgtcgttgac ggcggcctgg cgcaaaatct cctgcacgtc 8880 tcctgagttg tcttgatagg cgatctcggc catgaactgc tcgatctctt cctcctggag 8940 atctccgcgt ccggctcgct ccacggtggc ggcgaggtcg ttggaaatgc gggccatgag 9000 ctgcgagaag gcgttgaggc ctccctcgtt ccagacgcgg ctgtagacca cgcccccttc 9060 ggcatcgcgg gcgcgcatga ccacctgcgc gagattgagc tccacgtgcc gggcgaagac 9120 ggcgtagttt cgcaggcgct gaaagaggta gttgagggtg gtggcggtgt gttctgccac 9180 gaagaagtac ataacccagc gtcgcaacgt ggattcgttg atatccccca aggcctcaag 9240 gcgctccatg gcctcgtaga agtccacggc gaagttgaaa aactgggagt tgcgcgccga 9300 cacggttaac tcctcctcca gaagacggat gagctcggcg acagtgtcgc gcacctcgcg 9360 ctcaaaggct acaggggcct cttcttcttc ttcaatctcc tcttccataa gggcctcccc 9420 ttcttcttct tctggcggcg gtgggggagg ggggacacgg cggcgacgac ggcgcaccgg 9480 gaggcggtcg acaaagcgct cgatcatctc cccgcggcga cggcgcatgg tctcggtgac 9540 ggcgcggccg ttctcgcggg ggcgcagttg gaagacgccg cccgtcatgt cccggttatg 9600 ggttggcggg gggctgccat gcggcaggga tacggcgcta acgatgcatc tcaacaattg 9660 ttgtgtaggt actccgccgc cgagggacct gagcgagtcc gcatcgaccg gatcggaaaa 9720 cctctcgaga aaggcgtcta accagtcaca gtcgcaaggt aggctgagca ccgtggcggg 9780 cggcagcggg cggcggtcgg ggttgtttct ggcggaggtg ctgctgatga tgtaattaaa 9840 gtaggcggtc ttgagacggc ggatggtcga cagaagcacc atgtccttgg gtccggcctg 9900 ctgaatgcgc aggcggtcgg ccatgcccca ggcttcgttt tgacatcggc gcaggtcttt 9960 gtagtagtct tgcatgagcc tttctaccgg cacttcttct tctccttcct cttgtcctgc 10020 atctcttgca tctatcgctg cggcggcggc ggagtttggc cgtaggtggc gccctcttcc 10080 tcccatgcgt gtgaccccga agcccctcat cggctgaagc agggctaggt cggcgacaac 10140 gcgctcggct aatatggcct gctgcacctg cgtgagggta gactggaagt catccatgtc 10200 cacaaagcgg tggtatgcgc ccgtgttgat ggtgtaagtg cagttggcca taacggacca 10260 gttaacggtc tggtgacccg gctgcgagag ctcggtgtac ctgagacgcg agtaagccct 10320 cgagtcaaat acgtagtcgt tgcaagtccg caccaggtac tggtatccca ccaaaaagtg 10380 cggcggcggc tggcggtaga ggggccagcg tagggtggcc ggggctccgg gggcgagatc 10440 ttccaacata aggcgatgat atccgtagat gtacctggac atccaggtga tgccggcggc 10500 ggtggtggag gcgcgcggaa agtcgcggac gcggttccag atgttgcgca gcggcaaaaa 10560 gtgctccatg gtcgggacgc tctggccggt caggcgcgcg caatcgttga cgctctagac 10620 cgtgcaaaag gagagcctgt aagcgggcac tcttccgtgg tctggtggat aaattcgcaa 10680 gggtatcatg gcggacgacc ggggttcgag ccccgtatcc ggccgtccgc cgtgatccat 10740 gcggttaccg cccgcgtgtc gaacccaggt gtgcgacgtc agacaacggg ggagtgctcc 10800 ttttggcttc cttccaggcg cggcggctgc tgcgctagct tttttggcca ctggccgcgc 10860 gcagcgtaag cggttaggct ggaaagcgaa agcattaagt ggctcgctcc ctgtagccgg 10920 agggttattt tccaagggtt gagtcgcggg acccccggtt cgagtctcgg accggccgga 10980 ctgcggcgaa cgggggtttg cctccccgtc atgcaagacc ccgcttgcaa attcctccgg 11040 aaacagggac gagccccttt tttgcttttc ccagatgcat ccggtgctgc ggcagatgcg 11100 cccccctcct cagcagcggc aagagcaaga gcagcggcag acatgcaggg caccctcccc 11160 tcctcctacc gcgtcaggag gggcgacatc cgcggttgac gcggcagcag atggtgatta 11220 cgaacccccg cggcgccggg cccggcacta cctggacttg gaggagggcg agggcctggc 11280 gcggctagga gcgccctctc ctgagcggta cccaagggtg cagctgaagc gtgatacgcg 11340 tgaggcgtac gtgccgcggc agaacctgtt tcgcgaccgc gagggagagg agcccgagga 11400 gatgcgggat cgaaagttcc acgcagggcg cgagctgcgg catggcctga atcgcgagcg 11460 gttgctgcgc gaggaggact ttgagcccga cgcgcgaacc gggattagtc ccgcgcgcgc 11520 acacgtggcg gccgccgacc tggtaaccgc atacgagcag acggtgaacc aggagattaa 11580 ctttcaaaaa agctttaaca accacgtgcg tacgcttgtg gcgcgcgagg aggtggctat 11640 aggactgatg catctgtggg actttgtaag cgcgctggag caaaacccaa atagcaagcc 11700 gctcatggcg cagctgttcc ttatagtgca gcacagcagg gacaacgagg cattcaggga 11760 tgcgctgcta aacatagtag agcccgaggg ccgctggctg ctcgatttga taaacatcct 11820 gcagagcata gtggtgcagg agcgcagctt gagcctggct gacaaggtgg ccgccatcaa 11880 ctattccatg cttagcctgg gcaagtttta cgcccgcaag atataccata ccccttacgt 11940 tcccatagac aaggaggtaa agatcgaggg gttctacatg cgcatggcgc tgaaggtgct 12000 taccttgagc gacgacctgg gcgtttatcg caacgagcgc atccacaagg ccgtgagcgt 12060 gagccggcgg cgcgagctca gcgaccgcga gctgatgcac agcctgcaaa gggccctggc 12120 tggcacgggc agcggcgata gagaggccga gtcctacttt gacgcgggcg ctgacctgcg 12180 ctgggcccca agccgacgcg ccctggaggc agctggggcc ggacctgggc tggcggtggc 12240 acccgcgcgc gctggcaacg tcggcggcgt ggaggaatat gacgaggacg atgagtacga 12300 gccagaggac ggcgagtact aagcggtgat gtttctgatc agatgatgca agacgcaacg 12360 gacccggcgg tgcgggcggc gctgcagagc cagccgtccg gccttaactc cacggacgac 12420 tggcgccagg tcatggaccg catcatgtcg ctgactgcgc gcaatcctga cgcgttccgg 12480 cagcagccgc aggccaaccg gctctccgca attctggaag cggtggtccc ggcgcgcgca 12540 aaccccacgc acgagaaggt gctggcgatc gtaaacgcgc tggccgaaaa cagggccatc 12600 cggcccgacg aggccggcct ggtctacgac gcgctgcttc agcgcgtggc tcgttacaac 12660 agcggcaacg tgcagaccaa cctggaccgg ctggtggggg atgtgcgcga ggccgtggcg 12720 cagcgtgagc gcgcgcagca gcagggcaac ctgggctcca tggttgcact aaacgccttc 12780 ctgagtacac agcccgccaa cgtgccgcgg ggacaggagg actacaccaa ctttgtgagc 12840 gcactgcggc taatggtgac tgagacaccg caaagtgagg tgtaccagtc tgggccagac 12900 tattttttcc agaccagtag acaaggcctg cagaccgtaa acctgagcca ggctttcaaa 12960 aacttgcagg ggctgtgggg ggtgcgggct cccacaggcg accgcgcgac cgtgtctagc 13020 ttgctgacgc ccaactcgcg cctgttgctg ctgctaatag cgcccttcac ggacagtggc 13080 agcgtgtccc gggacacata cctaggtcac ttgctgacac tgtaccgcga ggccataggt 13140 caggcgcatg tggacgagca tactttccag gagattacaa gtgtcagccg cgcgctgggg 13200 caggaggaca cgggcagcct ggaggcaacc ctaaactacc tgctgaccaa ccggcggcag 13260 aagatcccct cgttgcacag tttaaacagc gaggaggagc gcattttgcg ctacgtgcag 13320 cagagcgtga gccttaacct gatgcgcgac ggggtaacgc ccagcgtggc gctggacatg 13380 accgcgcgca acatggaacc gggcatgtat gcctcaaacc ggccgtttat caaccgccta 13440 atggactact tgcatcgcgc ggccgccgtg aaccccgagt atttcaccaa tgccatcttg 13500 aacccgcact ggctaccgcc ccctggtttc tacaccgggg gattcgaggt gcccgagggt 13560 aacgatggat tcctctggga cgacatagac gacagcgtgt tttccccgca accgcagacc 13620 ctgctagagt tgcaacagcg cgagcaggca gaggcggcgc tgcgaaagga aagcttccgc 13680 aggccaagca gcttgtccga tctaggcgct gcggccccgc ggtcagatgc tagtagccca 13740 tttccaagct tgatagggtc tcttaccagc actcgcacca cccgcccgcg cctgctgggc 13800 gaggaggagt acctaaacaa ctcgctgctg cagccgcagc gcgaaaaaaa cctgcctccg 13860 gcatttccca acaacgggat agagagccta gtggacaaga tgagtagatg gaagacgtac 13920 gcgcaggagc acagggacgt gccaggcccg cgcccgccca cccgtcgtca aaggcacgac 13980 cgtcagcggg gtctggtgtg ggaggacgat gactcggcag acgacagcag cgtcctggat 14040 ttgggaggga gtggcaaccc gtttgcgcac cttcgcccca ggctggggag aatgttttaa 14100 aaaaaaaaaa gcatgatgca aaataaaaaa ctcaccaagg ccatggcacc gagcgttggt 14160 tttcttgtat tccccttagt atgcggcgcg cggcgatgta tgaggaaggt cctcctccct 14220 cctacgagag tgtggtgagc gcggcgccag tggcggcggc gctgggttct cccttcgatg 14280 ctcccctgga cccgccgttt gtgcctccgc ggtacctgcg gcctaccggg gggagaaaca 14340 gcatccgtta ctctgagttg gcacccctat tcgacaccac ccgtgtgtac ctggtggaca 14400 acaagtcaac ggatgtggca tccctgaact accagaacga ccacagcaac tttctgacca 14460 cggtcattca aaacaatgac tacagcccgg gggaggcaag cacacagacc atcaatcttg 14520 acgaccggtc gcactggggc ggcgacctga aaaccatcct gcataccaac atgccaaatg 14580 tgaacgagtt catgtttacc aataagttta aggcgcgggt gatggtgtcg cgcttgccta 14640 ctaaggacaa tcaggtggag ctgaaatacg agtgggtgga gttcacgctg cccgagggca 14700 actactccga gaccatgacc atagacctta tgaacaacgc gatcgtggag cactacttga 14760 aagtgggcag acagaacggg gttctggaaa gcgacatcgg ggtaaagttt gacacccgca 14820 acttcagact ggggtttgac cccgtcactg gtcttgtcat gcctggggta tatacaaacg 14880 aagccttcca tccagacatc attttgctgc caggatgcgg ggtggacttc acccacagcc 14940 gcctgagcaa cttgttgggc atccgcaagc ggcaaccctt ccaggagggc tttaggatca 15000 cctacgatga tctggagggt ggtaacattc ccgcactgtt ggatgtggac gcctaccagg 15060 cgagcttgaa agatgacacc gaacagggcg ggggtggcgc aggcggcagc aacagcagtg 15120 gcagcggcgc ggaagagaac tccaacgcgg cagccgcggc aatgcagccg gtggaggaca 15180 tgaacgatca tgccattcgc ggcgacacct ttgccacacg ggctgaggag aagcgcgctg 15240 aggccgaagc agcggccgaa gctgccgccc ccgctgcgca acccgaggtc gagaagcctc 15300 agaagaaacc ggtgatcaaa cccctgacag aggacagcaa gaaacgcagt tacaacctaa 15360 taagcaatga cagcaccttc acccagtacc gcagctggta ccttgcatac aactacggcg 15420 accctcagac cggaatccgc tcatggaccc tgctttgcac tcctgacgta acctgcggct 15480 cggagcaggt ctactggtcg ttgccagaca tgatgcaaga ccccgtgacc ttccgctcca 15540 cgcgccagat cagcaacttt ccggtggtgg gcgccgagct gttgcccgtg cactccaaga 15600 gcttctacaa cgaccaggcc gtctactccc aactcatccg ccagtttacc tctctgaccc 15660 acgtgttcaa tcgctttccc gagaaccaga ttttggcgcg cccgccagcc cccaccatca 15720 ccaccgtcag tgaaaacgtt cctgctctca cagatcacgg gacgctaccg ctgcgcaaca 15780 gcatcggagg agtccagcga gtgaccatta ctgacgccag acgccgcacc tgcccctacg 15840 tttacaaggc cctgggcata gtctcgccgc gcgtcctatc gagccgcact ttttgagcaa 15900 gcatgtccat ccttatatcg cccagcaata acacaggctg gggcctgcgc ttcccaagca 15960 agatgtttgg cggggccaag aagcgctccg accaacaccc agtgcgcgtg cgcgggcact 16020 accgcgcgcc ctggggcgcg cacaaacgcg gccgcactgg gcgcaccacc gtcgatgacg 16080 ccatcgacgc ggtggtggag gaggcgcgca actacacgcc cacgccgcca ccagtgtcca 16140 cagtggacgc ggccattcag accgtggtgc gcggagcccg gcgctatgct aaaatgaaga 16200 gacggcggag gcgcgtagca cgtcgccacc gccgccgacc cggcactgcc gcccaacgcg 16260 cggcggcggc cctgcttaac cgcgcacgtc gcaccggccg acgggcggcc atgcgggccg 16320 ctcgaaggct ggccgcgggt attgtcactg tgccccccag gtccaggcga cgagcggccg 16380 ccgcagcagc cgcggccatt agtgctatga ctcagggtcg caggggcaac gtgtattggg 16440 tgcgcgactc ggttagcggc ctgcgcgtgc ccgtgcgcac ccgccccccg cgcaactaga 16500 ttgcaagaaa aaactactta gactcgtact gttgtatgta tccagcggcg gcggcgcgca 16560 acgaagctat gtccaagcgc aaaatcaaag aagagatgct ccaggtcatc gcgccggaga 16620 tctatggccc cccgaagaag gaagagcagg attacaagcc ccgaaagcta aagcgggtca 16680 aaaagaaaaa gaaagatgat gatgatgaac ttgacgacga ggtggaactg ctgcacgcta 16740 ccgcgcccag gcgacgggta cagtggaaag gtcgacgcgt aaaacgtgtt ttgcgacccg 16800 gcaccaccgt agtctttacg cccggtgagc gctccacccg cacctacaag cgcgtgtatg 16860 atgaggtgta cggcgacgag gacctgcttg agcaggccaa cgagcgcctc ggggagtttg 16920 cctacggaaa gcggcataag gacatgctgg cgttgccgct ggacgagggc aacccaacac 16980 ctagcctaaa gcccgtaaca ctgcagcagg tgctgcccgc gcttgcaccg tccgaagaaa 17040 agcgcggcct aaagcgcgag tctggtgact tggcacccac cgtgcagctg atggtaccca 17100 agcgccagcg actggaagat gtcttggaaa aaatgaccgt ggaacctggg ctggagcccg 17160 aggtccgcgt gcggccaatc aagcaggtgg cgccgggact gggcgtgcag accgtggacg 17220 ttcagatacc cactaccagt agcaccagta ttgccaccgc cacagagggc atggagacac 17280 aaacgtcccc ggttgcctca gcggtggcgg atgccgcggt gcaggcggtc gctgcggccg 17340 cgtccaagac ctctacggag gtgcaaacgg acccgtggat gtttcgcgtt tcagcccccc 17400 ggcgcccgcg cggttcgagg aagtacggcg ccgccagcgc gctactgccc gaatatgccc 17460 tacatccttc cattgcgcct acccccggct atcgtggcta cacctaccgc cccagaagac 17520 gagcaactac ccgacgccga accaccactg gaacccgccg ccgccgtcgc cgtcgccagc 17580 ccgtgctggc cccgatttcc gtgcgcaggg tggctcgcga aggaggcagg accctggtgc 17640 tgccaacagc gcgctaccac cccagcatcg tttaaaagcc ggtctttgtg gttcttgcag 17700 atatggccct cacctgccgc ctccgtttcc cggtgccggg attccgagga agaatgcacc 17760 gtaggagggg catggccggc cacggcctga cgggcggcat gcgtcgtgcg caccaccggc 17820 ggcggcgcgc gtcgcaccgt cgcatgcgcg gcggtatcct gcccctcctt attccactga 17880 tcgccgcggc gattggcgcc gtgcccggaa ttgcatccgt ggccttgcag gcgcagagac 17940 actgattaaa aacaagttgc atgtggaaaa atcaaaataa aaagtctgga ctctcacgct 18000 cgcttggtcc tgtaactatt ttgtagaatg gaagacatca actttgcgtc tctggccccg 18060 cgacacggct cgcgcccgtt catgggaaac tggcaagata tcggcaccag caatatgagc 18120 ggtggcgcct tcagctgggg ctcgctgtgg agcggcatta aaaatttcgg ttccaccgtt 18180 aagaactatg gcagcaaggc ctggaacagc agcacaggcc agatgctgag ggataagttg 18240 aaagagcaaa atttccaaca aaaggtggta gatggcctgg cctctggcat tagcggggtg 18300 gtggacctgg ccaaccaggc agtgcaaaat aagattaaca gtaagcttga tccccgccct 18360 cccgtagagg agcctccacc ggccgtggag acagtgtctc cagaggggcg tggcgaaaag 18420 cgtccgcgcc ccgacaggga agaaactctg gtgacgcaaa tagacgagcc tccctcgtac 18480 gaggaggcac taaagcaagg cctgcccacc acccgtccca tcgcgcccat ggctaccgga 18540 gtgctgggcc agcacacacc cgtaacgctg gacctgcctc cccccgccga cacccagcag 18600 aaacctgtgc tgccaggccc gaccgccgtt gttgtaaccc gtcctagccg cgcgtccctg 18660 cgccgcgccg ccagcggtcc gcgatcgttg cggcccgtag ccagtggcaa ctggcaaagc 18720 acactgaaca gcatcgtggg tctgggggtg caatccctga agcgccgacg atgcttctga 18780 atagctaacg tgtcgtatgt gtgtcatgta tgcgtccatg tcgccgccag aggagctgct 18840 gagccgccgc gcgcccgctt tccaagatgg ctaccccttc gatgatgccg cagtggtctt 18900 acatgcacat ctcgggccag gacgcctcgg agtacctgag ccccgggctg gtgcagtttg 18960 cccgcgccac cgagacgtac ttcagcctga ataacaagtt tagaaacccc acggtggcgc 19020 ctacgcacga cgtgaccaca gaccggtccc agcgtttgac gctgcggttc atccctgtgg 19080 accgtgagga tactgcgtac tcgtacaagg cgcggttcac cctagctgtg ggtgataacc 19140 gtgtgctgga catggcttcc acgtactttg acatccgcgg cgtgctggac aggggcccta 19200 cttttaagcc ctactctggc actgcctaca acgccctggc tcccaagggt gccccaaatc 19260 cttgcgaatg ggatgaagct gctactgctc ttgaaataaa cctagaagaa gaggacgatg 19320 acaacgaaga cgaagtagac gagcaagctg agcagcaaaa aactcacgta tttgggcagg 19380 cgccttattc tggtataaat attacaaagg agggtattca aataggtgtc gaaggtcaaa 19440 cacctaaata tgccgataaa acatttcaac ctgaacctca aataggagaa tctcagtggt 19500 acgaaactga aattaatcat gcagctggga gagtccttaa aaagactacc ccaatgaaac 19560 catgttacgg ttcatatgca aaacccacaa atgaaaatgg agggcaaggc attcttgtaa 19620 agcaacaaaa tggaaagcta gaaagtcaag tggaaatgca atttttctca actactgagg 19680 cgaccgcagg caatggtgat aacttgactc ctaaagtggt attgtacagt gaagatgtag 19740 atatagaaac cccagacact catatttctt acatgcccac tattaaggaa ggtaactcac 19800 gagaactaat gggccaacaa tctatgccca acaggcctaa ttacattgct tttagggaca 19860 attttattgg tctaatgtat tacaacagca cgggtaatat gggtgttctg gcgggccaag 19920 catcgcagtt gaatgctgtt gtagatttgc aagacagaaa cacagagctt tcataccagc 19980 ttttgcttga ttccattggt gatagaacca ggtacttttc tatgtggaat caggctgttg 20040 acagctatga tccagatgtt agaattattg aaaatcatgg aactgaagat gaacttccaa 20100 attactgctt tccactggga ggtgtgatta atacagagac tcttaccaag gtaaaaccta 20160 aaacaggtca ggaaaatgga tgggaaaaag atgctacaga attttcagat aaaaatgaaa 20220 taagagttgg aaataatttt gccatggaaa tcaatctaaa tgccaacctg tggagaaatt 20280 tcctgtactc caacatagcg ctgtatttgc ccgacaagct aaagtacagt ccttccaacg 20340 taaaaatttc tgataaccca aacacctacg actacatgaa caagcgagtg gtggctcccg 20400 ggttagtgga ctgctacatt aaccttggag cacgctggtc ccttgactat atggacaacg 20460 tcaacccatt taaccaccac cgcaatgctg gcctgcgcta ccgctcaatg ttgctgggca 20520 atggtcgcta tgtgcccttc cacatccagg tgcctcagaa gttctttgcc attaaaaacc 20580 tccttctcct gccgggctca tacacctacg agtggaactt caggaaggat gttaacatgg 20640 ttctgcagag ctccctagga aatgacctaa gggttgacgg agccagcatt aagtttgata 20700 gcatttgcct ttacgccacc ttcttcccca tggcccacaa caccgcctcc acgcttgagg 20760 ccatgcttag aaacgacacc aacgaccagt cctttaacga ctatctctcc gccgccaaca 20820 tgctctaccc tatacccgcc aacgctacca acgtgcccat atccatcccc tcccgcaact 20880 gggcggcttt ccgcggctgg gccttcacgc gccttaagac taaggaaacc ccatcactgg 20940 gctcgggcta cgacccttat tacacctact ctggctctat accctaccta gatggaacct 21000 tttacctcaa ccacaccttt aagaaggtgg ccattacctt tgactcttct gtcagctggc 21060 ctggcaatga ccgcctgctt acccccaacg agtttgaaat taagcgctca gttgacgggg 21120 agggttacaa cgttgcccag tgtaacatga ccaaagactg gttcctggta caaatgctag 21180 ctaactacaa cattggctac cagggcttct atatcccaga gagctacaag gaccgcatgt 21240 actccttctt tagaaacttc cagcccatga gccgtcaggt ggtggatgat actaaataca 21300 aggactacca acaggtgggc atcctacacc aacacaacaa ctctggattt gttggctacc 21360 ttgcccccac catgcgcgaa ggacaggcct accctgctaa cttcccctat ccgcttatag 21420 gcaagaccgc agttgacagc attacccaga aaaagtttct ttgcgatcgc accctttggc 21480 gcatcccatt ctccagtaac tttatgtcca tgggcgcact cacagacctg ggccaaaacc 21540 ttctctacgc caactccgcc cacgcgctag acatgacttt tgaggtggat cccatggacg 21600 agcccaccct tctttatgtt ttgtttgaag tctttgacgt ggtccgtgtg caccggccgc 21660 accgcggcgt catcgaaacc gtgtacctgc gcacgccctt ctcggccggc aacgccacaa 21720 cataaagaag caagcaacat caacaacagc tgccgccatg ggctccagtg agcaggaact 21780 gaaagccatt gtcaaagatc ttggttgtgg gccatatttt ttgggcacct atgacaagcg 21840 ctttccaggc tttgtttctc cacacaagct cgcctgcgcc atagtcaata cggccggtcg 21900 cgagactggg ggcgtacact ggatggcctt tgcctggaac ccgcactcaa aaacatgcta 21960 cctctttgag ccctttggct tttctgacca gcgactcaag caggtttacc agtttgagta 22020 cgagtcactc ctgcgccgta gcgccattgc ttcttccccc gaccgctgta taacgctgga 22080 aaagtccacc caaagcgtac aggggcccaa ctcggccgcc tgtggactat tctgctgcat 22140 gtttctccac gcctttgcca actggcccca aactcccatg gatcacaacc ccaccatgaa 22200 ccttattacc ggggtaccca actccatgct caacagtccc caggtacagc ccaccctgcg 22260 tcgcaaccag gaacagctct acagcttcct ggagcgccac tcgccctact tccgcagcca 22320 cagtgcgcag attaggagcg ccacttcttt ttgtcacttg aaaaacatgt aaaaataatg 22380 tactagagac actttcaata aaggcaaatg cttttatttg tacactctcg ggtgattatt 22440 tacccccacc cttgccgtct gcgccgttta aaaatcaaag gggttctgcc gcgcatcgct 22500 atgcgccact ggcagggaca cgttgcgata ctggtgttta gtgctccact taaactcagg 22560 cacaaccatc cgcggcagct cggtgaagtt ttcactccac aggctgcgca ccatcaccaa 22620 cgcgtttagc aggtcgggcg ccgatatctt gaagtcgcag ttggggcctc cgccctgcgc 22680 gcgcgagttg cgatacacag ggttgcagca ctggaacact atcagcgccg ggtggtgcac 22740 gctggccagc acgctcttgt cggagatcag atccgcgtcc aggtcctccg cgttgctcag 22800 ggcgaacgga gtcaactttg gtagctgcct tcccaaaaag ggcgcgtgcc caggctttga 22860 gttgcactcg caccgtagtg gcatcaaaag gtgaccgtgc ccggtctggg cgttaggata 22920 cagcgcctgc ataaaagcct tgatctgctt aaaagccacc tgagcctttg cgccttcaga 22980 gaagaacatg ccgcaagact tgccggaaaa ctgattggcc ggacaggccg cgtcgtgcac 23040 gcagcacctt gcgtcggtgt tggagatctg caccacattt cggccccacc ggttcttcac 23100 gatcttggcc ttgctagact gctccttcag cgcgcgctgc ccgttttcgc tcgtcacatc 23160 catttcaatc acgtgctcct tatttatcat aatgcttccg tgtagacact taagctcgcc 23220 ttcgatctca gcgcagcggt gcagccacaa cgcgcagccc gtgggctcgt gatgcttgta 23280 ggtcacctct gcaaacgact gcaggtacgc ctgcaggaat cgccccatca tcgtcacaaa 23340 ggtcttgttg ctggtgaagg tcagctgcaa cccgcggtgc tcctcgttca gccaggtctt 23400 gcatacggcc gccagagctt ccacttggtc aggcagtagt ttgaagttcg cctttagatc 23460 gttatccacg tggtacttgt ccatcagcgc gcgcgcagcc tccatgccct tctcccacgc 23520 agacacgatc ggcacactca gcgggttcat caccgtaatt tcactttccg cttcgctggg 23580 ctcttcctct tcctcttgcg tccgcatacc acgcgccact gggtcgtctt cattcagccg 23640 ccgcactgtg cgcttacctc ctttgccatg cttgattagc accggtgggt tgctgaaacc 23700 caccatttgt agcgccacat cttctctttc ttcctcgctg tccacgatta cctctggtga 23760 tggcgggcgc tcgggcttgg gagaagggcg cttctttttc ttcttgggcg caatggccaa 23820 atccgccgcc gaggtcgatg gccgcgggct gggtgtgcgc ggcaccagcg cgtcttgtga 23880 tgagtcttcc tcgtcctcgg actcgatacg ccgcctcatc cgcttttttg ggggcgcccg 23940 gggaggcggc ggcgacgggg acggggacga cacgtcctcc atggttgggg gacgtcgcgc 24000 cgcaccgcgt ccgcgctcgg gggtggtttc gcgctgctcc tcttcccgac tggccatttc 24060 cttctcctat aggcagaaaa agatcatgga gtcagtcgag aagaaggaca gcctaaccgc 24120 cccctctgag ttcgccacca ccgcctccac cgatgccgcc aacgcgccta ccaccttccc 24180 cgtcgaggca cccccgcttg aggaggagga agtgattatc gagcaggacc caggttttgt 24240 aagcgaagac gacgaggacc gctcagtacc aacagaggat aaaaagcaag accaggacaa 24300 cgcagaggca aacgaggaac aagtcgggcg gggggacgaa aggcatggcg actacctaga 24360 tgtgggagac gacgtgctgt tgaagcatct gcagcgccag tgcgccatta tctgcgacgc 24420 gttgcaagag cgcagcgatg tgcccctcgc catagcggat gtcagccttg cctacgaacg 24480 ccacctattc tcaccgcgcg taccccccaa acgccaagaa aacggcacat gcgagcccaa 24540 cccgcgcctc aacttctacc ccgtatttgc cgtgccagag gtgcttgcca cctatcacat 24600 ctttttccaa aactgcaaga tacccctatc ctgccgtgcc aaccgcagcc gagcggacaa 24660 gcagctggcc ttgcggcagg gcgctgtcat acctgatatc gcctcgctca acgaagtgcc 24720 aaaaatcttt gagggtcttg gacgcgacga gaagcgcgcg gcaaacgctc tgcaacagga 24780 aaacagcgaa aatgaaagtc actctggagt gttggtggaa ctcgagggtg acaacgcgcg 24840 cctagccgta ctaaaacgca gcatcgaggt cacccacttt gcctacccgg cacttaacct 24900 accccccaag gtcatgagca cagtcatgag tgagctgatc gtgcgccgtg cgcagcccct 24960 ggagagggat gcaaatttgc aagaacaaac agaggagggc ctacccgcag ttggcgacga 25020 gcagctagcg cgctggcttc aaacgcgcga gcctgccgac ttggaggagc gacgcaaact 25080 aatgatggcc gcagtgctcg ttaccgtgga gcttgagtgc atgcagcggt tctttgctga 25140 cccggagatg cagcgcaagc tagaggaaac attgcactac acctttcgac agggctacgt 25200 acgccaggcc tgcaagatct ccaacgtgga gctctgcaac ctggtctcct accttggaat 25260 tttgcacgaa aaccgccttg ggcaaaacgt gcttcattcc acgctcaagg gcgaggcgcg 25320 ccgcgactac gtccgcgact gcgtttactt atttctatgc tacacctggc agacggccat 25380 gggcgtttgg cagcagtgct tggaggagtg caacctcaag gagctgcaga aactgctaaa 25440 gcaaaacttg aaggacctat ggacggcctt caacgagcgc tccgtggccg cgcacctggc 25500 ggacatcatt ttccccgaac gcctgcttaa aaccctgcaa cagggtctgc cagacttcac 25560 cagtcaaagc atgttgcaga actttaggaa ctttatccta gagcgctcag gaatcttgcc 25620 cgccacctgc tgtgcacttc ctagcgactt tgtgcccatt aagtaccgcg aatgccctcc 25680 gccgctttgg ggccactgct accttctgca gctagccaac taccttgcct accactctga 25740 cataatggaa gacgtgagcg gtgacggtct actggagtgt cactgtcgct gcaacctatg 25800 caccccgcac cgctccctgg tttgcaattc gcagctgctt aacgaaagtc aaattatcgg 25860 tacctttgag ctgcagggtc cctcgcctga cgaaaagtcc gcggctccgg ggttgaaact 25920 cactccgggg ctgtggacgt cggcttacct tcgcaaattt gtacctgagg actaccacgc 25980 ccacgagatt aggttctacg aagaccaatc ccgcccgcca aatgcggagc ttaccgcctg 26040 cgtcattacc cagggccaca ttcttggcca attgcaagcc atcaacaaag cccgccaaga 26100 gtttctgcta cgaaagggac ggggggttta cttggacccc cagtccggcg aggagctcaa 26160 cccaatcccc ccgccgccgc agccctatca gcagcagccg cgggcccttg cttcccagga 26220 tggcacccaa aaagaagctg cagctgccgc cgccacccac ggacgaggag gaatactggg 26280 acagtcaggc agaggaggtt ttggacgagg aggaggagga catgatggaa gactgggaga 26340 gcctagacga ggaagcttcc gaggtcgaag aggtgtcaga cgaaacaccg tcaccctcgg 26400 tcgcattccc ctcgccggcg ccccagaaat cggcaaccgg ttccagcatg gctacaacct 26460 ccgctcctca ggcgccgccg gcactgcccg ttcgccgacc caaccgtaga tgggacacca 26520 ctggaaccag ggccggtaag tccaagcagc cgccgccgtt agcccaagag caacaacagc 26580 gccaaggcta ccgctcatgg cgcgggcaca agaacgccat agttgcttgc ttgcaagact 26640 gtgggggcaa catctccttc gcccgccgct ttcttctcta ccatcacggc gtggccttcc 26700 cccgtaacat cctgcattac taccgtcatc tctacagccc atactgcacc ggcggcagcg 26760 gcagcggcag caacagcagc ggccacacag aagcaaaggc gaccggatag caagactctg 26820 acaaagccca agaaatccac agcggcggca gcagcaggag gaggagcgct gcgtctggcg 26880 cccaacgaac ccgtatcgac ccgcgagctt agaaacagga tttttcccac tctgtatgct 26940 atatttcaac agagcagggg ccaagaacaa gagctgaaaa taaaaaacag gtctctgcga 27000 tccctcaccc gcagctgcct gtatcacaaa agcgaagatc agcttcggcg cacgctggaa 27060 gacgcggagg ctctcttcag taaatactgc gcgctgactc ttaaggacta gtttcgcgcc 27120 ctttctcaaa tttaagcgcg aaaactacgt catctccagc ggccacaccc ggcgccagca 27180 cctgtcgtca gcgccatttc aactttgtat acaaaagttg tgatgagcaa ggaaattccc 27240 acgccctaca tgtggagtta ccagccacaa atgggacttg cggctggagc tgcccaagac 27300 tactcaaccc gaataaacta catgagcgcg ggaccccaca tgatatcccg ggtcaacgga 27360 atacgcgccc accgaaaccg aattctcctg gaacaggcgg ctattaccac cacacctcgt 27420 aataacctta atccccgtag ttggcccgct gccctggtgt accaggaaag tcccgctccc 27480 accactgtgg tacttcccag agacgcccag gccgaagttc agatgactaa ctcaggggcg 27540 cagcttgcgg gcggctttcg tcacagggtg cggtcgcccg ggcagggtat aactcacctg 27600 acaatcagag ggcgaggtat tcagctcaac gacgagtcgg tgagctcctc gcttggtctc 27660 cgtccggacg ggacatttca gatcggcggc gccggccgct cttcattcac gcctcgtcag 27720 gcaatcctaa ctctgcagac ctcgtcctct gagccgcgct ctggaggcat tggaactctg 27780 caatttattg aggagtttgt gccatcggtc tactttaacc ccttctcggg acctcccggc 27840 cactatccgg atcaatttat tcctaacttt gacgcggtaa aggactcggc ggacggctac 27900 gactgaatgt taagtggaga ggcagagcaa ctgcgcctga aacacctggt ccactgtcgc 27960 cgccacaagt gctttgcccg cgactccggt gagttttgct actttgaatt gcccgaggat 28020 catatcgagg gcccggcgca cggcgtccgg cttaccgccc agggagagct tgcccgtagc 28080 ctgattcggg agtttaccca gcgccccctg ctagttgagc gggacagggg accctgtgtt 28140 ctcactgtga tttgcaactg tcctaaccct ggattacatc aagatctttg ttgccatctc 28200 tgtgctgagt ataataaata cagaaattaa aatatactgg ggctcctatc gccatcctgt 28260 aaacgccacc gtcttcaccc gcccaagcaa accaaggcga accttacctg gtacttttaa 28320 catctctccc tctgtgattt acaacagttt caacccagac ggagtgagtc tacgagagaa 28380 cctctccgag ctcagctact ccatcagaaa aaacaccacc ctccttacct gccgggaacg 28440 tacgagtgcg tcaccggccg ctgcaccaca cctaccgcct gaccgtaaac cagacttttt 28500 ccggacagac ctcaataact ctgtttacca gaacaggagg tgagcttaga aaacccttag 28560 ggtattaggc caaaggcgca gctactgtgg ggtttatgaa caattcaagc aactctacgg 28620 gctattctaa ttcaggtttc tctagaatcg gggttggggt tattctctgt cttgtgattc 28680 tctttattct tatactaacg cttctctgcc taaggctcgc cgcctgctgt gtgcacattt 28740 gcatttattg tcagcttttt aaacgctggg gtcgccaccc aagatgatta ggtacataat 28800 cctaggttta ctcacccttg cgtcagccca cggtaccacc caaaaggtgg attttaagga 28860 gccagcctgt aatgttacat tcgcagctga agctaatgag tgcaccactc ttataaaatg 28920 caccacagaa catgaaaagc tgcttattcg ccacaaaaac aaaattggca agtatgctgt 28980 ttatgctatt tggcagccag gtgacactac agagtataat gttacagttt tccagggtaa 29040 aagtcataaa acttttatgt atacttttcc attttatgaa atgtgcgaca ttaccatgta 29100 catgagcaaa cagtataagt tgtggccccc acaaaattgt gtggaaaaca ctggcacttt 29160 ctgctgcact gctatgctaa ttacagtgct cgctttggtc tgtaccctac tctatattaa 29220 atacaaaagc agacgcagct ttattgagga aaagaaaatg ccttaattta ctaagttaca 29280 aagctaatgt caccactaac tgctttactc gctgcttgca aaacaaattc aaaaagttag 29340 cattataatt agaataggat ttaaaccccc cggtcatttc ctgctcaata ccattcccct 29400 gaacaattga ctctatgtgg gatatgctcc agcgctacaa ccttgaagtc aggcttcctg 29460 gatgtcagca tctgactttg gccagcacct gtcccgcgga tttgttccag tccaactaca 29520 gcgacccacc ctaacagaga tgaccaacac aaccaacgcg gccgccgcta ccggacttac 29580 atctaccaca aatacacccc aagtttctgc ctttgtcaat aactgggata acttgggcat 29640 gtggtggttc tccatagcgc ttatgtttgt atgccttatt attatgtggc tcatctgctg 29700 cctaaagcgc aaacgcgccc gaccacccat ctatagtccc atcattgtgc tacacccaaa 29760 caatgatgga atccatagat tggacggact gaaacacatg ttcttttctc ttacagtatg 29820 attaaatgag acatgattcc tcgagttttt atattactga cccttgttgc gcttttttgt 29880 gcgtgctcca cattggctgc ggtttctcac atcgaagtag actgcattcc agccttcaca 29940 gtctatttgc tttacggatt tgtcaccctc acgctcatct gcagcctcat cactgtggtc 30000 atcgccttta tccagtgcat tgactgggtc tgtgtgcgct ttgcatatct cagacaccat 30060 ccccagtaca gggacaggac tatagctgag cttcttagaa ttctttaatt atgaaattta 30120 ctgtgacttt tctgctgatt atttgcaccc tatctgcgtt ttgttccccg acctccaagc 30180 ctcaaagaca tatatcatgc agattcactc gtatatggaa tattccaagt tgctacaatg 30240 aaaaaagcga tctttccgaa gcctggttat atgcaatcat ctctgttatg gtgttctgca 30300 gtaccatctt agccctagct atatatccct accttgacat tggctggaac gcaatagatg 30360 ccatgaacca cccaactttc cccgcgcccg ctatgcttcc actgcaacaa gttgttgccg 30420 gcggctttgt cccagccaat cagcctcgcc caccttctcc cacccccact gaaatcagct 30480 actttaatct aacaggagga gatgactgac accctagatc tagaaatgga cggaattatt 30540 acagagcagc gcctgctaga aagacgcagg gcagcggccg agcaacagcg catgaatcaa 30600 gagctccaag acatggttaa cttgcaccag tgcaaaaggg gtatcttttg tctggtaaag 30660 caggccaaag tcacctacga cagtaatacc accggacacc gccttagcta caagttgcca 30720 accaagcgtc agaaattggt ggtcatggtg ggagaaaagc ccattaccat aactcagcac 30780 tcggtagaaa ccgaaggctg cattcactca ccttgtcaag gacctgagga tctctgcacc 30840 cttattaaga ccctgtgcgg tctcaaagat cttattccct ttaactaata aaaaaaaata 30900 ataaagcatc acttacttaa aatcagttag caaatttctg tccagtttat tcagcagcac 30960 ctccttgccc tcctcccagc tctggtattg cagcttcctc ctggctgcaa actttctcca 31020 caatctaaat ggaatgtcag tttcctcctg ttcctgtcca tccgcaccca ctatcttcat 31080 gttgttgcag atgaagcgcg caagaccgtc tgaagatacc ttcaaccccg tgtatccata 31140 tgacacggaa accggtcctc caactgtgcc ttttcttact cctccctttg tatcccccaa 31200 tgggtttcaa gagagtcccc ctggggtact ctctttgcgc ctatccgaac ctctagttac 31260 ctccaatggc atgcttgcgc tcaaaatggg caacggcctc tctctggacg aggccggcaa 31320 ccttacctcc caaaatgtaa ccactgtgag cccacctctc aaaaaaacca agtcaaacat 31380 aaacctggaa atatctgcac ccctcacagt tacctcagaa gccctaactg tggctgccgc 31440 cgcacctcta atggtcgcgg gcaacacact caccatgcaa tcacaggccc cgctaaccgt 31500 gcacgactcc aaacttagca ttgccaccca aggacccctc acagtgtcag aaggaaagct 31560 agccctgcaa acatcaggcc ccctcaccac caccgatagc agtaccctta ctatcactgc 31620 ctcaccccct ctaactactg ccactggtag cttgggcatt gacttgaaag agcccattta 31680 tacacaaaat ggaaaactag gactaaagta cggggctcct ttgcatgtaa cagacgacct 31740 aaacactttg accgtagcaa ctggtccagg tgtgactatt aataatactt ccttgcaaac 31800 taaagttact ggagccttgg gttttgattc acaaggcaat atgcaactta atgtagcagg 31860 aggactaagg attgattctc aaaacagacg ccttatactt gatgttagtt atccgtttga 31920 tgctcaaaac caactaaatc taagactagg acagggccct ctttttataa actcagccca 31980 caacttggat attaactaca acaaaggcct ttacttgttt acagcttcaa acaattccaa 32040 aaagcttgag gttaacctaa gcactgccaa ggggttgatg tttgacgcta cagccatagc 32100 cattaatgca ggagatgggc ttgaatttgg ttcacctaat gcaccaaaca caaatcccct 32160 caaaacaaaa attggccatg gcctagaatt tgattcaaac aaggctatgg ttcctaaact 32220 aggaactggc cttagttttg acagcacagg tgccattaca gtaggaaaca aaaataatga 32280 taagctaact ttgtggacca caccagctcc atctcctaac tgtagactaa atgcagagaa 32340 agatgctaaa ctcactttgg tcttaacaaa atgtggcagt caaatacttg ctacagtttc 32400 agttttggct gttaaaggca gtttggctcc aatatctgga acagttcaaa gtgctcatct 32460 tattataaga tttgacgaaa atggagtgct actaaacaat tccttcctgg acccagaata 32520 ttggaacttt agaaatggag atcttactga aggcacagcc tatacaaacg ctgttggatt 32580 tatgcctaac ctatcagctt atccaaaatc tcacggtaaa actgccaaaa gtaacattgt 32640 cagtcaagtt tacttaaacg gagacaaaac taaacctgta acactaacca ttacactaaa 32700 cggtacacag gaaacaggag acacaactcc aagtgcatac tctatgtcat tttcatggga 32760 ctggtctggc cacaactaca ttaatgaaat atttgccaca tcctcttaca ctttttcata 32820 cattgcccaa gaataaagaa tcgtttgtgt tatgtttcaa cgtgtttatt tttcaattgc 32880 agaaaatttc aagtcatttt tcattcagta gtatagcccc accaccacat agcttataca 32940 gatcaccgta cctcaacttt gtataataaa gttgaatcaa actcacagaa ccctagtatt 33000 caacctgcca cctccctccc aacacacaga gtacacagtc ctttctcccc ggctggcctt 33060 aaaaagcatc atatcatggg taacagacat attcttaggt gttatattcc acacggtttc 33120 ctgtcgagcc aaacgctcat cagtgatatt aataaactcc ccgggcagct cacttaagtt 33180 catgtcgctg tccagctgct gagccacagg ctgctgtcca acttgcggtt gcttaacggg 33240 cggcgaagga gaagtccacg cctacatggg ggtagagtca taatcgtgca tcaggatagg 33300 gcggtggtgc tgcagcagcg cgcgaataaa ctgctgccgc cgccgctccg tcctgcagga 33360 atacaacatg gcagtggtct cctcagcgat gattcgcacc gcccgcagca taaggcgcct 33420 tgtcctccgg gcacagcagc gcaccctgat ctcacttaaa tcagcacagt aactgcagca 33480 cagcaccaca atattgttca aaatcccaca gtgcaaggcg ctgtatccaa agctcatggc 33540 ggggaccaca gaacccacgt ggccatcata ccacaagcgc aggtagatta agtggcgacc 33600 cctcataaac acgctggaca taaacattac ctcttttggc atgttgtaat tcaccacctc 33660 ccggtaccat ataaacctct gattaaacat ggcgccatcc accaccatcc taaaccagct 33720 ggccaaaacc tgcccgccgg ctatacactg cagggaaccg ggactggaac aatgacagtg 33780 gagagcccag gactcgtaac catggatcat catgctcgtc atgatatcaa tgttggcaca 33840 acacaggcac acgtgcatac acttcctcag gattacaagc tcctcccgcg ttagaaccat 33900 atcccaggga acaacccatt cctgaatcag cgtaaatccc acactgcagg gaagacctcg 33960 cacgtaactc acgttgtgca ttgtcaaagt gttacattcg ggcagcagcg gatgatcctc 34020 cagtatggta gcgcgggttt ctgtctcaaa aggaggtaga cgatccctac tgtacggagt 34080 gcgccgagac aaccgagatc gtgttggtcg tagtgtcatg ccaaatggaa cgccggacgt 34140 agtcatattt cctgaagcaa aaccaggtgc gggcgtgaca aacagatctg cgtctccggt 34200 ctcgccgctt agatcgctct gtgtagtagt tgtagtatat ccactctctc aaagcatcca 34260 ggcgccccct ggcttcgggt tctatgtaaa ctccttcatg cgccgctgcc ctgataacat 34320 ccaccaccgc agaataagcc acacccagcc aacctacaca ttcgttctgc gagtcacaca 34380 cgggaggagc gggaagagct ggaagaacca tgtttttttt tttattccaa aagattatcc 34440 aaaacctcaa aatgaagatc tattaagtga acgcgctccc ctccggtggc gtggtcaaac 34500 tctacagcca aagaacagat aatggcattt gtaagatgtt gcacaatggc ttccaaaagg 34560 caaacggccc tcacgtccaa gtggacgtaa aggctaaacc cttcagggtg aatctcctct 34620 ataaacattc cagcaccttc aaccatgccc aaataattct catctcgcca ccttctcaat 34680 atatctctaa gcaaatcccg aatattaagt ccggccattg taaaaatctg ctccagagcg 34740 ccctccacct tcagcctcaa gcagcgaatc atgattgcaa aaattcaggt tcctcacaga 34800 cctgtataag attcaaaagc ggaacattaa caaaaatacc gcgatcccgt aggtcccttc 34860 gcagggccag ctgaacataa tcgtgcaggt ctgcacggac cagcgcggcc acttccccgc 34920 caggaaccat gacaaaagaa cccacactga ttatgacacg catactcgga gctatgctaa 34980 ccagcgtagc cccgatgtaa gcttgttgca tgggcggcga tataaaatgc aaggtgctgc 35040 tcaaaaaatc aggcaaagcc tcgcgcaaaa aagaaagcac atcgtagtca tgctcatgca 35100 gataaaggca ggtaagctcc ggaaccacca cagaaaaaga caccattttt ctctcaaaca 35160 ttgggccagg attctcctcg acgtcaccag cctgcttcag caggctgaag ttagtagctc 35220 cgcttccctt gtacagctcg tccatgccgc cggtggagtg gcggccctcg gcgcgttcgt 35280 actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccaacttg atgttgacgt 35340 tgtaggcgcc gggcagctgc acgggcttct tggccttgta ggtggtcttg acctcagcgt 35400 cgtagtggcc gccgtccttc agcttcagcc tctgcttgat ctcgcccttc agggcgccgt 35460 cctcggggta catccgctcg gaggaggcct cccagcccat ggtcttcttc tgcattacgg 35520 ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct 35580 gcagggagga gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct 35640 cccacttgaa gccctcgggg aaggacagct tcaagtagtc ggggatgtcg gcggggtgct 35700 tcacgtaggc cttggagccg tacatgaact gaggggacag gatgtcccag gcgaagggca 35760 gggggccacc cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct 35820 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga 35880 agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc accatgtctg 35940 cgggtttctg cataaacaca aaataaaata acaaaaaaac atttaaacat tagaagcctg 36000 tcttacaaca ggaaaaacaa cccttataag cataagacgg actacggcca tgccggcgtg 36060 accgtaaaaa aactggtcac cgtgattaaa aagcaccacc gacagctcct cggtcatgtc 36120 cggagtcata atgtaagact cggtaaacac atcaggttga ttcacatcgg tcagtgctaa 36180 aaagcgaccg aaatagcccg ggggaataca tacccgcagg cgtagagaca acattacagc 36240 ccccatagga ggtataacaa aattaatagg agagaaaaac acataaacac ctgaaaaacc 36300 ctcctgccta ggcaaaatag caccctcccg ctccagaaca acatacagcg cttccacagc 36360 ggcagccata acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa aacaccactc 36420 gacacggcac cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag agcgagtata 36480 tataggacta aaaaatgacg taacggttaa agtccacaaa aaacacccag aaaaccgcac 36540 gcgaacctac gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa tcgtcacttc 36600 cgttttccca cgttacgtca cttcccattt taagaaaact acaattccca acacatacaa 36660 gttactccgc cctaaaacct acgtcacccg ccccgttccc acgccccgcg ccacgtcaca 36720 aactccaccc cctcattatc atattggctt caatccaaaa taaggtatat tattgatgat 36780 g 36781 <210> 12 <211> 36899 <212> DNA <213> Artificial Sequence <220> <223> Synthetic adenovirus genome YPet-P2A-Fiber <400> 12 attaccctgt tatccctaca tcatcaataa tataccttat tttggattga agccaatatg 60 ataatgaggg ggtggagttt gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta 120 gtagtgtggc ggaagtgtga tgttgcaagt gtggcggaac acatgtaagc gacggatgtg 180 gcaaaagtga cgtttttggt gtgcgccggt gtacacagga agtgacaatt ttcgcgcggt 240 tttaggcgga tgttgtagta aatttgggcg taaccgagta agatttggcc attttcgcgg 300 gaaaactgaa taagaggaag tgaaatctga ataattttgt gttactcata gcgcgtaata 360 tttgtctagg gccgcgggga ctttgaccgt ttacgtggag actcgcccag gtgtttttct 420 caggtgtttt ccgcgttccg ggtcaaagtt ggcgttttat tattatagtc agctgacgtg 480 tagtgtattt atacccggtg agttcctcaa gaggccactc ttgagtgcca gcgagtagag 540 ttttctcctc cgagccgctc cgacaccggg actgaaaatg agacatatta tctgccacgg 600 aggtgttatt accgaagaaa tggccgccag tcttttggac cagctgatcg aagaggtact 660 ggctgataat cttccacctc ctagccattt tgaaccacct acccttcacg aactgtatga 720 tttagacgtg acggcccccg aagatcccaa cgaggaggcg gtttcgcaga tttttcccga 780 ctctgtaatg ttggcggtgc aggaagggat tgacttactc acttttccgc cggcgcccgg 840 ttctccggag ccgcctcacc tttcccggca gcccgagcag ccggagcaga gagccttggg 900 tccggtttct atgccaaacc ttgtaccgga ggtgatcgat cttacctgcc acgaggctgg 960 ctttccaccc agtgacgacg aggatgaaga gggtgaggag tttgtgttag attatgtgga 1020 gcaccccggg cacggttgca ggtcttgtca ttatcaccgg aggaatacgg gggacccaga 1080 tattatgtgt tcgctttgct atatgaggac ctgtggcatg tttgtctaca gtaagtgaaa 1140 attatgggca gtgggtgata gagtggtggg tttggtgtgg taattttttt tttaattttt 1200 acagttttgt ggtttaaaga attttgtatt gtgatttttt taaaaggtcc tgtgtctgaa 1260 cctgagcctg agcccgagcc agaaccggag cctgcaagac ctacccgccg tcctaaaatg 1320 gcgcctgcta tcctgagacg cccgacatca cctgtgtcta gagaatgcaa tagtagtacg 1380 gatagctgtg actccggtcc ttctaacaca cctcctgaga tacacccggt ggtcccgctg 1440 tgccccatta aaccagttgc cgtgagagtt ggtgggcgtc gccaggctgt ggaatgtatc 1500 gaggacttgc ttaacgagcc tgggcaacct ttggacttga gctgtaaacg ccccaggcca 1560 taaggtgtaa acctgtgatt gcgtgtgtgg ttaacgcctt tgtttgctga atgagttgat 1620 gtaagtttaa taaagggtga gataatgttt aacttgcatg gcgtgttaaa tggggcgggg 1680 cttaaagggt atataatgcg ccgtgggcta atcttggtta catctgacct catggaggct 1740 tgggagtgtt tggaagattt ttctgctgtg cgtaacttgc tggaacagag ctctaacagt 1800 acctcttggt tttggaggtt tctgtggggc tcatcccagg caaagttagt ctgcagaatt 1860 aaggaggatt acaagtggga atttgaagag cttttgaaat cctgtggtga gctgtttgat 1920 tctttgaatc tgggtcacca ggcgcttttc caagagaagg tcatcaagac tttggatttt 1980 tccacaccgg ggcgcgctgc ggctgctgtt gcttttttga gttttataaa ggataaatgg 2040 agcgaagaaa cccatctgag cggggggtac ctgctggatt ttctggccat gcatctgtgg 2100 agagcggttg tgagacacaa gaatcgcctg ctactgttgt cttccgtccg cccggcgata 2160 ataccgacgg aggagcagca gcagcagcag gaggaagcca ggcggcggcg gcaggagcag 2220 agcccatgga acccgagagc cggcctggac cctcgggaat gaatgttgta caggtggctg 2280 aactgtatcc agaactgaga cgcattttga caattacaga ggatgggcag gggctaaagg 2340 gggtaaagag ggagcggggg gcttgtgagg ctacagagga ggctaggaat ctagctttta 2400 gcttaatgac cagacaccgt cctgagtgta ttacttttca acagatcaag gataattgcg 2460 ctaatgagct tgatctgctg gcgcagaagt attccataga gcagctgacc acttactggc 2520 tgcagccagg ggatgatttt gaggaggcta ttagggtata tgcaaaggtg gcacttaggc 2580 cagattgcaa gtacaagatc agcaaacttg taaatatcag gaattgttgc tacatttctg 2640 ggaacggggc cgaggtggag atagatacgg aggatagggt ggcctttaga tgtagcatga 2700 taaatatgtg gccgggggtg cttggcatgg acggggtggt tattatgaat gtaaggttta 2760 ctggccccaa ttttagcggt acggttttcc tggccaatac caaccttatc ctacacggtg 2820 taagcttcta tgggtttaac aatacctgtg tggaagcctg gaccgatgta agggttcggg 2880 gctgtgcctt ttactgctgc tggaaggggg tggtgtgtcg ccccaaaagc agggcttcaa 2940 ttaagaaatg cctctttgaa aggtgtacct tgggtatcct gtctgagggt aactccaggg 3000 tgcgccacaa tgtggcctcc gactgtggtt gcttcatgct agtgaaaagc gtggctgtga 3060 ttaagcataa catggtatgt ggcaactgcg aggacagggc ctctcagatg ctgacctgct 3120 cggacggcaa ctgtcacctg ctgaagacca ttcacgtagc cagccactct cgcaaggcct 3180 ggccagtgtt tgagcataac atactgaccc gctgttcctt gcatttgggt aacaggaggg 3240 gggtgttcct accttaccaa tgcaatttga gtcacactaa gatattgctt gagcccgaga 3300 gcatgtccaa ggtgaacctg aacggggtgt ttgacatgac catgaagatc tggaaggtgc 3360 tgaggtacga tgagacccgc accaggtgca gaccctgcga gtgtggcggt aaacatatta 3420 ggaaccagcc tgtgatgctg gatgtgaccg aggagctgag gcccgatcac ttggtgctgg 3480 cctgcacccg cgctgagttt ggctctagcg atgaagatac agattgaggt actgaaatgt 3540 gtgggcgtgg cttaagggtg ggaaagaata tataaggtgg gggtcttatg tagttttgta 3600 tctgttttgc agcagccgcc gccgcccaac ttttctatac aaagttgtaa tgagcaccaa 3660 ctcgtttgat ggaagcattg tgagctcata tttgacaacg cgcatgcccc catgggccgg 3720 ggtgcgtcag aatgtgatgg gctccagcat tgatggtcgc cccgtcctgc ccgcaaactc 3780 tactaccttg acctacgaga ccgtgtctgg aacgccgttg gagactgcag cctccgccgc 3840 cgcttcagcc gctgcagcca ccgcccgcgg gattgtgact gactttgctt tcctgagccc 3900 gcttgcaagc agtgcagctt cccgttcatc cgcccgcgat gacaagttga cggctctttt 3960 ggcacaattg gattctttga cccgggaact taatgtcgtt tctcagcagc tgttggatct 4020 gcgccagcag gtttctgccc tgaaggcttc ctcccctccc aatgcggttt aaaacataaa 4080 taaaaaacca gactctgttt ggatttggat caagcataag tgtcttgctg tctttattta 4140 ggggttttgc gcgcgcggta ggcccgggac cagcggtctc ggtcgttgag ggtcctgtgt 4200 attttttcca ggacgtggta aaggtgactc tggatgttca gatacatggg cataagcccg 4260 tctctggggt ggaggtagca ccactgcaga gcttcatgct gcggggtggt gttgtagatg 4320 atccagtcgt agcaggagcg ctgggcgtgg tgcctaaaaa tgtctttcag tagcaagctg 4380 attgccaggg gcaggccctt ggtgtaagtg tttacaaagc ggttaagctg ggatgggtgc 4440 atacgtgggg atatgagatg catcttggac tgtattttta ggttggctat gttcccagcc 4500 atatccctcc ggggattcat gttgtgcaga accaccagca cagtgtatcc ggtgcacttg 4560 ggaaatttgt catgtagctt agaaggaaat gcgtggaaga acttggagac gcccttgtga 4620 cctccaagat tttccatgca ttcgtccata atgatggcaa tgggcccacg ggcggcggcc 4680 tgggcgaaga tatttctggg atcactaacg tcatagttgt gttccaggat gagatcgtca 4740 taggccattt ttacaaagcg cgggcggagg gtgccagact gcggtataat ggttccatcc 4800 ggcccagggg cgtagttacc ctcacagatt tgcatttccc acgctttgag ttcagatggg 4860 gggatcatgt ctacctgcgg ggcgatgaag aaaacggttt ccggggtagg ggagatcagc 4920 tgggaagaaa gcaggttcct gagcagctgc gacttaccgc agccggtggg cccgtaaatc 4980 acacctatta ccgggtgcaa ctggtagtta agagagctgc agctgccgtc atccctgagc 5040 aggggggcca cttcgttaag catgtccctg actcgcatgt tttccctgac caaatccgcc 5100 agaaggcgct cgccgcccag cgatagcagt tcttgcaagg aagcaaagtt tttcaacggt 5160 ttgagaccgt ccgccgtagg catgcttttg agcgtttgac caagcagttc caggcggtcc 5220 cacagctcgg tcacctgctc tacggcatct cgatccagca tatctcctcg tttcgcgggt 5280 tggggcggct ttcgctgtac ggcagtagtc ggtgctcgtc cagacgggcc agggtcatgt 5340 ctttccacgg gcgcagggtc ctcgtcagcg tagtctgggt cacggtgaag gggtgcgctc 5400 cgggctgcgc gctggccagg gtgcgcttga ggctggtcct gctggtgctg aagcgctgcc 5460 ggtcttcgcc ctgcgcgtcg gccaggtagc atttgaccat ggtgtcatag tccagcccct 5520 ccgcggcgtg gcccttggcg cgcagcttgc ccttggagga ggcgccgcac gaggggcagt 5580 gcagactttt gagggcgtag agcttgggcg cgagaaatac cgattccggg gagtaggcat 5640 ccgcgccgca ggccccgcag acggtctcgc attccacgag ccaggtgagc tctggccgtt 5700 cggggtcaaa aaccaggttt cccccatgct ttttgatgcg tttcttacct ctggtttcca 5760 tgagccggtg tccacgctcg gtgacgaaaa ggctgtccgt gtccccgtat acagacttga 5820 gaggcctgtc ctcgagcggt gttccgcggt cctcctcgta tagaaactcg gaccactctg 5880 agacaaaggc tcgcgtccag gccagcacga aggaggctaa gtgggagggg tagcggtcgt 5940 tgtccactag ggggtccact cgctccaggg tgtgaagaca catgtcgccc tcttcggcat 6000 caaggaaggt gattggtttg taggtgtagg ccacgtgacc gggtgttcct gaaggggggc 6060 tataaaaggg ggtgggggcg cgttcgtcct cactctcttc cgcatcgctg tctgcgaggg 6120 ccagctgttg gggtgagtac tccctctgaa aagcgggcat gacttctgcg ctaagattgt 6180 cagtttccaa aaacgaggag gatttgatat tcacctggcc cgcggtgatg cctttgaggg 6240 tggccgcatc catctggtca gaaaagacaa tctttttgtt gtcaagcttg gtggcaaacg 6300 acccgtagag ggcgttggac agcaacttgg cgatggagcg cagggtttgg tttttgtcgc 6360 gatcggcgcg ctccttggcc gcgatgttta gctgcacgta ttcgcgcgca acgcaccgcc 6420 attcgggaaa gacggtggtg cgctcgtcgg gcaccaggtg cacgcgccaa ccgcggttgt 6480 gcagggtgac aaggtcaacg ctggtggcta cctctccgcg taggcgctcg ttggtccagc 6540 agaggcggcc gcccttgcgc gagcagaatg gcggtagggg gtctagctgc gtctcgtccg 6600 gggggtctgc gtccacggta aagaccccgg gcagcaggcg cgcgtcgaag tagtctatct 6660 tgcatccttg caagtctagc gcctgctgcc atgcgcgggc ggcaagcgcg cgctcgtatg 6720 ggttgagtgg gggaccccat ggcatggggt gggtgagcgc ggaggcgtac atgccgcaaa 6780 tgtcgtaaac gtagaggggc tctctgagta ttccaagata tgtagggtag catcttccac 6840 cgcggatgct ggcgcgcacg taatcgtata gttcgtgcga gggagcgagg aggtcgggac 6900 cgaggttgct acgggcgggc tgctctgctc ggaagactat ctgcctgaag atggcatgtg 6960 agttggatga tatggttgga cgctggaaga cgttgaagct ggcgtctgtg agacctaccg 7020 cgtcacgcac gaaggaggcg taggagtcgc gcagcttgtt gaccagctcg gcggtgacct 7080 gcacgtctag ggcgcagtag tccagggttt ccttgatgat gtcatactta tcctgtccct 7140 tttttttcca cagctcgcgg ttgaggacaa actcttcgcg gtctttccag tactcttgga 7200 tcggaaaccc gtcggcctcc gaacggtaag agcctagcat gtagaactgg ttgacggcct 7260 ggtaggcgca gcatcccttt tctacgggta gcgcgtatgc ctgcgcggcc ttccggagcg 7320 aggtgtgggt gagcgcaaag gtgtccctga ccatgacttt gaggtactgg tatttgaagt 7380 cagtgtcgtc gcatccgccc tgctcccaga gcaaaaagtc cgtgcgcttt ttggaacgcg 7440 gatttggcag ggcgaaggtg acatcgttga agagtatctt tcccgcgcga ggcataaagt 7500 tgcgtgtgat gcggaagggt cccggcacct cggaacggtt gttaattacc tgggcggcga 7560 gcacgatctc gtcaaagccg ttgatgttgt ggcccacaat gtaaagttcc aagaagcgcg 7620 ggatgccctt gatggaaggc aattttttaa gttcctcgta ggtgagctct tcaggggagc 7680 tgagcccgtg ctctgaaagg gcccagtctg caagatgagg gttggaagcg acgaatgagc 7740 tccacaggtc acgggccatt agcatttgca ggtggtcgcg aaaggtccta aactggcgac 7800 ctatggccat tttttctggg gtgatgcagt agaaggtaag cgggtcttgt tcccagcggt 7860 cccatccaag gttcgcggct aggtctcgcg cggcagtcac tagaggctca tctccgccga 7920 acttcatgac cagcatgaag ggcacgagct gcttcccaaa ggcccccatc caagtatagg 7980 tctctacatc gtaggtgaca aagagacgct cggtgcgagg atgcgagccg atcgggaaga 8040 actggatctc ccgccaccaa ttggaggagt ggctattgat gtggtgaaag tagaagtccc 8100 tgcgacgggc cgaacactcg tgctggcttt tgtaaaaacg tgcgcagtac tggcagcggt 8160 gcacgggctg tacatcctgc acgaggttga cctgacgacc gcgcacaagg aagcagagtg 8220 ggaatttgag cccctcgcct ggcgggtttg gctggtggtc ttctacttcg gctgcttgtc 8280 cttgaccgtc tggctgctcg aggggagtta cggtggatcg gaccaccacg ccgcgcgagc 8340 ccaaagtcca gatgtccgcg cgcggcggtc ggagcttgat gacaacatcg cgcagatggg 8400 agctgtccat ggtctggagc tcccgcggcg tcaggtcagg cgggagctcc tgcaggttta 8460 cctcgcatag acgggtcagg gcgcgggcta gatccaggtg atacctaatt tccaggggct 8520 ggttggtggc ggcgtcgatg gcttgcaaga ggccgcatcc ccgcggcgcg actacggtac 8580 cgcgcggcgg gcggtgggcc gcgggggtgt ccttggatga tgcatctaaa agcggtgacg 8640 cgggcgagcc cccggaggta gggggggctc cggacccgcc gggagagggg gcaggggcac 8700 gtcggcgccg cgcgcgggca ggagctggtg ctgcgcgcgt aggttgctgg cgaacgcgac 8760 gacgcggcgg ttgatctcct gaatctggcg cctctgcgtg aagacgacgg gcccggtgag 8820 cttgagcctg aaagagagtt cgacagaatc aatttcggtg tcgttgacgg cggcctggcg 8880 caaaatctcc tgcacgtctc ctgagttgtc ttgataggcg atctcggcca tgaactgctc 8940 gatctcttcc tcctggagat ctccgcgtcc ggctcgctcc acggtggcgg cgaggtcgtt 9000 ggaaatgcgg gccatgagct gcgagaaggc gttgaggcct ccctcgttcc agacgcggct 9060 gtagaccacg cccccttcgg catcgcgggc gcgcatgacc acctgcgcga gattgagctc 9120 cacgtgccgg gcgaagacgg cgtagtttcg caggcgctga aagaggtagt tgagggtggt 9180 ggcggtgtgt tctgccacga agaagtacat aacccagcgt cgcaacgtgg attcgttgat 9240 atcccccaag gcctcaaggc gctccatggc ctcgtagaag tccacggcga agttgaaaaa 9300 ctgggagttg cgcgccgaca cggttaactc ctcctccaga agacggatga gctcggcgac 9360 agtgtcgcgc acctcgcgct caaaggctac aggggcctct tcttcttctt caatctcctc 9420 ttccataagg gcctcccctt cttcttcttc tggcggcggt gggggagggg ggacacggcg 9480 gcgacgacgg cgcaccggga ggcggtcgac aaagcgctcg atcatctccc cgcggcgacg 9540 gcgcatggtc tcggtgacgg cgcggccgtt ctcgcggggg cgcagttgga agacgccgcc 9600 cgtcatgtcc cggttatggg ttggcggggg gctgccatgc ggcagggata cggcgctaac 9660 gatgcatctc aacaattgtt gtgtaggtac tccgccgccg agggacctga gcgagtccgc 9720 atcgaccgga tcggaaaacc tctcgagaaa ggcgtctaac cagtcacagt cgcaaggtag 9780 gctgagcacc gtggcgggcg gcagcgggcg gcggtcgggg ttgtttctgg cggaggtgct 9840 gctgatgatg taattaaagt aggcggtctt gagacggcgg atggtcgaca gaagcaccat 9900 gtccttgggt ccggcctgct gaatgcgcag gcggtcggcc atgccccagg cttcgttttg 9960 acatcggcgc aggtctttgt agtagtcttg catgagcctt tctaccggca cttcttcttc 10020 tccttcctct tgtcctgcat ctcttgcatc tatcgctgcg gcggcggcgg agtttggccg 10080 taggtggcgc cctcttcctc ccatgcgtgt gaccccgaag cccctcatcg gctgaagcag 10140 ggctaggtcg gcgacaacgc gctcggctaa tatggcctgc tgcacctgcg tgagggtaga 10200 ctggaagtca tccatgtcca caaagcggtg gtatgcgccc gtgttgatgg tgtaagtgca 10260 gttggccata acggaccagt taacggtctg gtgacccggc tgcgagagct cggtgtacct 10320 gagacgcgag taagccctcg agtcaaatac gtagtcgttg caagtccgca ccaggtactg 10380 gtatcccacc aaaaagtgcg gcggcggctg gcggtagagg ggccagcgta gggtggccgg 10440 ggctccgggg gcgagatctt ccaacataag gcgatgatat ccgtagatgt acctggacat 10500 ccaggtgatg ccggcggcgg tggtggaggc gcgcggaaag tcgcggacgc ggttccagat 10560 gttgcgcagc ggcaaaaagt gctccatggt cgggacgctc tggccggtca ggcgcgcgca 10620 atcgttgacg ctctagaccg tgcaaaagga gagcctgtaa gcgggcactc ttccgtggtc 10680 tggtggataa attcgcaagg gtatcatggc ggacgaccgg ggttcgagcc ccgtatccgg 10740 ccgtccgccg tgatccatgc ggttaccgcc cgcgtgtcga acccaggtgt gcgacgtcag 10800 acaacggggg agtgctcctt ttggcttcct tccaggcgcg gcggctgctg cgctagcttt 10860 tttggccact ggccgcgcgc agcgtaagcg gttaggctgg aaagcgaaag cattaagtgg 10920 ctcgctccct gtagccggag ggttattttc caagggttga gtcgcgggac ccccggttcg 10980 agtctcggac cggccggact gcggcgaacg ggggtttgcc tccccgtcat gcaagacccc 11040 gcttgcaaat tcctccggaa acagggacga gccccttttt tgcttttccc agatgcatcc 11100 ggtgctgcgg cagatgcgcc cccctcctca gcagcggcaa gagcaagagc agcggcagac 11160 atgcagggca ccctcccctc ctcctaccgc gtcaggaggg gcgacatccg cggttgacgc 11220 ggcagcagat ggtgattacg aacccccgcg gcgccgggcc cggcactacc tggacttgga 11280 ggagggcgag ggcctggcgc ggctaggagc gccctctcct gagcggtacc caagggtgca 11340 gctgaagcgt gatacgcgtg aggcgtacgt gccgcggcag aacctgtttc gcgaccgcga 11400 gggagaggag cccgaggaga tgcgggatcg aaagttccac gcagggcgcg agctgcggca 11460 tggcctgaat cgcgagcggt tgctgcgcga ggaggacttt gagcccgacg cgcgaaccgg 11520 gattagtccc gcgcgcgcac acgtggcggc cgccgacctg gtaaccgcat acgagcagac 11580 ggtgaaccag gagattaact ttcaaaaaag ctttaacaac cacgtgcgta cgcttgtggc 11640 gcgcgaggag gtggctatag gactgatgca tctgtgggac tttgtaagcg cgctggagca 11700 aaacccaaat agcaagccgc tcatggcgca gctgttcctt atagtgcagc acagcaggga 11760 caacgaggca ttcagggatg cgctgctaaa catagtagag cccgagggcc gctggctgct 11820 cgatttgata aacatcctgc agagcatagt ggtgcaggag cgcagcttga gcctggctga 11880 caaggtggcc gccatcaact attccatgct tagcctgggc aagttttacg cccgcaagat 11940 ataccatacc ccttacgttc ccatagacaa ggaggtaaag atcgaggggt tctacatgcg 12000 catggcgctg aaggtgctta ccttgagcga cgacctgggc gtttatcgca acgagcgcat 12060 ccacaaggcc gtgagcgtga gccggcggcg cgagctcagc gaccgcgagc tgatgcacag 12120 cctgcaaagg gccctggctg gcacgggcag cggcgataga gaggccgagt cctactttga 12180 cgcgggcgct gacctgcgct gggccccaag ccgacgcgcc ctggaggcag ctggggccgg 12240 acctgggctg gcggtggcac ccgcgcgcgc tggcaacgtc ggcggcgtgg aggaatatga 12300 cgaggacgat gagtacgagc cagaggacgg cgagtactaa gcggtgatgt ttctgatcag 12360 atgatgcaag acgcaacgga cccggcggtg cgggcggcgc tgcagagcca gccgtccggc 12420 cttaactcca cggacgactg gcgccaggtc atggaccgca tcatgtcgct gactgcgcgc 12480 aatcctgacg cgttccggca gcagccgcag gccaaccggc tctccgcaat tctggaagcg 12540 gtggtcccgg cgcgcgcaaa ccccacgcac gagaaggtgc tggcgatcgt aaacgcgctg 12600 gccgaaaaca gggccatccg gcccgacgag gccggcctgg tctacgacgc gctgcttcag 12660 cgcgtggctc gttacaacag cggcaacgtg cagaccaacc tggaccggct ggtgggggat 12720 gtgcgcgagg ccgtggcgca gcgtgagcgc gcgcagcagc agggcaacct gggctccatg 12780 gttgcactaa acgccttcct gagtacacag cccgccaacg tgccgcgggg acaggaggac 12840 tacaccaact ttgtgagcgc actgcggcta atggtgactg agacaccgca aagtgaggtg 12900 taccagtctg ggccagacta ttttttccag accagtagac aaggcctgca gaccgtaaac 12960 ctgagccagg ctttcaaaaa cttgcagggg ctgtgggggg tgcgggctcc cacaggcgac 13020 cgcgcgaccg tgtctagctt gctgacgccc aactcgcgcc tgttgctgct gctaatagcg 13080 cccttcacgg acagtggcag cgtgtcccgg gacacatacc taggtcactt gctgacactg 13140 taccgcgagg ccataggtca ggcgcatgtg gacgagcata ctttccagga gattacaagt 13200 gtcagccgcg cgctggggca ggaggacacg ggcagcctgg aggcaaccct aaactacctg 13260 ctgaccaacc ggcggcagaa gatcccctcg ttgcacagtt taaacagcga ggaggagcgc 13320 attttgcgct acgtgcagca gagcgtgagc cttaacctga tgcgcgacgg ggtaacgccc 13380 agcgtggcgc tggacatgac cgcgcgcaac atggaaccgg gcatgtatgc ctcaaaccgg 13440 ccgtttatca accgcctaat ggactacttg catcgcgcgg ccgccgtgaa ccccgagtat 13500 ttcaccaatg ccatcttgaa cccgcactgg ctaccgcccc ctggtttcta caccggggga 13560 ttcgaggtgc ccgagggtaa cgatggattc ctctgggacg acatagacga cagcgtgttt 13620 tccccgcaac cgcagaccct gctagagttg caacagcgcg agcaggcaga ggcggcgctg 13680 cgaaaggaaa gcttccgcag gccaagcagc ttgtccgatc taggcgctgc ggccccgcgg 13740 tcagatgcta gtagcccatt tccaagcttg atagggtctc ttaccagcac tcgcaccacc 13800 cgcccgcgcc tgctgggcga ggaggagtac ctaaacaact cgctgctgca gccgcagcgc 13860 gaaaaaaacc tgcctccggc atttcccaac aacgggatag agagcctagt ggacaagatg 13920 agtagatgga agacgtacgc gcaggagcac agggacgtgc caggcccgcg cccgcccacc 13980 cgtcgtcaaa ggcacgaccg tcagcggggt ctggtgtggg aggacgatga ctcggcagac 14040 gacagcagcg tcctggattt gggagggagt ggcaacccgt ttgcgcacct tcgccccagg 14100 ctggggagaa tgttttaaaa aaaaaaaagc atgatgcaaa ataaaaaact caccaaggcc 14160 atggcaccga gcgttggttt tcttgtattc cccttagtat gcggcgcgcg gcgatgtatg 14220 aggaaggtcc tcctccctcc tacgagagtg tggtgagcgc ggcgccagtg gcggcggcgc 14280 tgggttctcc cttcgatgct cccctggacc cgccgtttgt gcctccgcgg tacctgcggc 14340 ctaccggggg gagaaacagc atccgttact ctgagttggc acccctattc gacaccaccc 14400 gtgtgtacct ggtggacaac aagtcaacgg atgtggcatc cctgaactac cagaacgacc 14460 acagcaactt tctgaccacg gtcattcaaa acaatgacta cagcccgggg gaggcaagca 14520 cacagaccat caatcttgac gaccggtcgc actggggcgg cgacctgaaa accatcctgc 14580 ataccaacat gccaaatgtg aacgagttca tgtttaccaa taagtttaag gcgcgggtga 14640 tggtgtcgcg cttgcctact aaggacaatc aggtggagct gaaatacgag tgggtggagt 14700 tcacgctgcc cgagggcaac tactccgaga ccatgaccat agaccttatg aacaacgcga 14760 tcgtggagca ctacttgaaa gtgggcagac agaacggggt tctggaaagc gacatcgggg 14820 taaagtttga cacccgcaac ttcagactgg ggtttgaccc cgtcactggt cttgtcatgc 14880 ctggggtata tacaaacgaa gccttccatc cagacatcat tttgctgcca ggatgcgggg 14940 tggacttcac ccacagccgc ctgagcaact tgttgggcat ccgcaagcgg caacccttcc 15000 aggagggctt taggatcacc tacgatgatc tggagggtgg taacattccc gcactgttgg 15060 atgtggacgc ctaccaggcg agcttgaaag atgacaccga acagggcggg ggtggcgcag 15120 gcggcagcaa cagcagtggc agcggcgcgg aagagaactc caacgcggca gccgcggcaa 15180 tgcagccggt ggaggacatg aacgatcatg ccattcgcgg cgacaccttt gccacacggg 15240 ctgaggagaa gcgcgctgag gccgaagcag cggccgaagc tgccgccccc gctgcgcaac 15300 ccgaggtcga gaagcctcag aagaaaccgg tgatcaaacc cctgacagag gacagcaaga 15360 aacgcagtta caacctaata agcaatgaca gcaccttcac ccagtaccgc agctggtacc 15420 ttgcatacaa ctacggcgac cctcagaccg gaatccgctc atggaccctg ctttgcactc 15480 ctgacgtaac ctgcggctcg gagcaggtct actggtcgtt gccagacatg atgcaagacc 15540 ccgtgacctt ccgctccacg cgccagatca gcaactttcc ggtggtgggc gccgagctgt 15600 tgcccgtgca ctccaagagc ttctacaacg accaggccgt ctactcccaa ctcatccgcc 15660 agtttacctc tctgacccac gtgttcaatc gctttcccga gaaccagatt ttggcgcgcc 15720 cgccagcccc caccatcacc accgtcagtg aaaacgttcc tgctctcaca gatcacggga 15780 cgctaccgct gcgcaacagc atcggaggag tccagcgagt gaccattact gacgccagac 15840 gccgcacctg cccctacgtt tacaaggccc tgggcatagt ctcgccgcgc gtcctatcga 15900 gccgcacttt ttgagcaagc atgtccatcc ttatatcgcc cagcaataac acaggctggg 15960 gcctgcgctt cccaagcaag atgtttggcg gggccaagaa gcgctccgac caacacccag 16020 tgcgcgtgcg cgggcactac cgcgcgccct ggggcgcgca caaacgcggc cgcactgggc 16080 gcaccaccgt cgatgacgcc atcgacgcgg tggtggagga ggcgcgcaac tacacgccca 16140 cgccgccacc agtgtccaca gtggacgcgg ccattcagac cgtggtgcgc ggagcccggc 16200 gctatgctaa aatgaagaga cggcggaggc gcgtagcacg tcgccaccgc cgccgacccg 16260 gcactgccgc ccaacgcgcg gcggcggccc tgcttaaccg cgcacgtcgc accggccgac 16320 gggcggccat gcgggccgct cgaaggctgg ccgcgggtat tgtcactgtg ccccccaggt 16380 ccaggcgacg agcggccgcc gcagcagccg cggccattag tgctatgact cagggtcgca 16440 ggggcaacgt gtattgggtg cgcgactcgg ttagcggcct gcgcgtgccc gtgcgcaccc 16500 gccccccgcg caactagatt gcaagaaaaa actacttaga ctcgtactgt tgtatgtatc 16560 cagcggcggc ggcgcgcaac gaagctatgt ccaagcgcaa aatcaaagaa gagatgctcc 16620 aggtcatcgc gccggagatc tatggccccc cgaagaagga agagcaggat tacaagcccc 16680 gaaagctaaa gcgggtcaaa aagaaaaaga aagatgatga tgatgaactt gacgacgagg 16740 tggaactgct gcacgctacc gcgcccaggc gacgggtaca gtggaaaggt cgacgcgtaa 16800 aacgtgtttt gcgacccggc accaccgtag tctttacgcc cggtgagcgc tccacccgca 16860 cctacaagcg cgtgtatgat gaggtgtacg gcgacgagga cctgcttgag caggccaacg 16920 agcgcctcgg ggagtttgcc tacggaaagc ggcataagga catgctggcg ttgccgctgg 16980 acgagggcaa cccaacacct agcctaaagc ccgtaacact gcagcaggtg ctgcccgcgc 17040 ttgcaccgtc cgaagaaaag cgcggcctaa agcgcgagtc tggtgacttg gcacccaccg 17100 tgcagctgat ggtacccaag cgccagcgac tggaagatgt cttggaaaaa atgaccgtgg 17160 aacctgggct ggagcccgag gtccgcgtgc ggccaatcaa gcaggtggcg ccgggactgg 17220 gcgtgcagac cgtggacgtt cagataccca ctaccagtag caccagtatt gccaccgcca 17280 cagagggcat ggagacacaa acgtccccgg ttgcctcagc ggtggcggat gccgcggtgc 17340 aggcggtcgc tgcggccgcg tccaagacct ctacggaggt gcaaacggac ccgtggatgt 17400 ttcgcgtttc agccccccgg cgcccgcgcg gttcgaggaa gtacggcgcc gccagcgcgc 17460 tactgcccga atatgcccta catccttcca ttgcgcctac ccccggctat cgtggctaca 17520 cctaccgccc cagaagacga gcaactaccc gacgccgaac caccactgga acccgccgcc 17580 gccgtcgccg tcgccagccc gtgctggccc cgatttccgt gcgcagggtg gctcgcgaag 17640 gaggcaggac cctggtgctg ccaacagcgc gctaccaccc cagcatcgtt taaaagccgg 17700 tctttgtggt tcttgcagat atggccctca cctgccgcct ccgtttcccg gtgccgggat 17760 tccgaggaag aatgcaccgt aggaggggca tggccggcca cggcctgacg ggcggcatgc 17820 gtcgtgcgca ccaccggcgg cggcgcgcgt cgcaccgtcg catgcgcggc ggtatcctgc 17880 ccctccttat tccactgatc gccgcggcga ttggcgccgt gcccggaatt gcatccgtgg 17940 ccttgcaggc gcagagacac tgattaaaaa caagttgcat gtggaaaaat caaaataaaa 18000 agtctggact ctcacgctcg cttggtcctg taactatttt gtagaatgga agacatcaac 18060 tttgcgtctc tggccccgcg acacggctcg cgcccgttca tgggaaactg gcaagatatc 18120 ggcaccagca atatgagcgg tggcgccttc agctggggct cgctgtggag cggcattaaa 18180 aatttcggtt ccaccgttaa gaactatggc agcaaggcct ggaacagcag cacaggccag 18240 atgctgaggg ataagttgaa agagcaaaat ttccaacaaa aggtggtaga tggcctggcc 18300 tctggcatta gcggggtggt ggacctggcc aaccaggcag tgcaaaataa gattaacagt 18360 aagcttgatc cccgccctcc cgtagaggag cctccaccgg ccgtggagac agtgtctcca 18420 gaggggcgtg gcgaaaagcg tccgcgcccc gacagggaag aaactctggt gacgcaaata 18480 gacgagcctc cctcgtacga ggaggcacta aagcaaggcc tgcccaccac ccgtcccatc 18540 gcgcccatgg ctaccggagt gctgggccag cacacacccg taacgctgga cctgcctccc 18600 cccgccgaca cccagcagaa acctgtgctg ccaggcccga ccgccgttgt tgtaacccgt 18660 cctagccgcg cgtccctgcg ccgcgccgcc agcggtccgc gatcgttgcg gcccgtagcc 18720 agtggcaact ggcaaagcac actgaacagc atcgtgggtc tgggggtgca atccctgaag 18780 cgccgacgat gcttctgaat agctaacgtg tcgtatgtgt gtcatgtatg cgtccatgtc 18840 gccgccagag gagctgctga gccgccgcgc gcccgctttc caagatggct accccttcga 18900 tgatgccgca gtggtcttac atgcacatct cgggccagga cgcctcggag tacctgagcc 18960 ccgggctggt gcagtttgcc cgcgccaccg agacgtactt cagcctgaat aacaagttta 19020 gaaaccccac ggtggcgcct acgcacgacg tgaccacaga ccggtcccag cgtttgacgc 19080 tgcggttcat ccctgtggac cgtgaggata ctgcgtactc gtacaaggcg cggttcaccc 19140 tagctgtggg tgataaccgt gtgctggaca tggcttccac gtactttgac atccgcggcg 19200 tgctggacag gggccctact tttaagccct actctggcac tgcctacaac gccctggctc 19260 ccaagggtgc cccaaatcct tgcgaatggg atgaagctgc tactgctctt gaaataaacc 19320 tagaagaaga ggacgatgac aacgaagacg aagtagacga gcaagctgag cagcaaaaaa 19380 ctcacgtatt tgggcaggcg ccttattctg gtataaatat tacaaaggag ggtattcaaa 19440 taggtgtcga aggtcaaaca cctaaatatg ccgataaaac atttcaacct gaacctcaaa 19500 taggagaatc tcagtggtac gaaactgaaa ttaatcatgc agctgggaga gtccttaaaa 19560 agactacccc aatgaaacca tgttacggtt catatgcaaa acccacaaat gaaaatggag 19620 ggcaaggcat tcttgtaaag caacaaaatg gaaagctaga aagtcaagtg gaaatgcaat 19680 ttttctcaac tactgaggcg accgcaggca atggtgataa cttgactcct aaagtggtat 19740 tgtacagtga agatgtagat atagaaaccc cagacactca tatttcttac atgcccacta 19800 ttaaggaagg taactcacga gaactaatgg gccaacaatc tatgcccaac aggcctaatt 19860 acattgcttt tagggacaat tttattggtc taatgtatta caacagcacg ggtaatatgg 19920 gtgttctggc gggccaagca tcgcagttga atgctgttgt agatttgcaa gacagaaaca 19980 cagagctttc ataccagctt ttgcttgatt ccattggtga tagaaccagg tacttttcta 20040 tgtggaatca ggctgttgac agctatgatc cagatgttag aattattgaa aatcatggaa 20100 ctgaagatga acttccaaat tactgctttc cactgggagg tgtgattaat acagagactc 20160 ttaccaaggt aaaacctaaa acaggtcagg aaaatggatg ggaaaaagat gctacagaat 20220 tttcagataa aaatgaaata agagttggaa ataattttgc catggaaatc aatctaaatg 20280 ccaacctgtg gagaaatttc ctgtactcca acatagcgct gtatttgccc gacaagctaa 20340 agtacagtcc ttccaacgta aaaatttctg ataacccaaa cacctacgac tacatgaaca 20400 agcgagtggt ggctcccggg ttagtggact gctacattaa ccttggagca cgctggtccc 20460 ttgactatat ggacaacgtc aacccattta accaccaccg caatgctggc ctgcgctacc 20520 gctcaatgtt gctgggcaat ggtcgctatg tgcccttcca catccaggtg cctcagaagt 20580 tctttgccat taaaaacctc cttctcctgc cgggctcata cacctacgag tggaacttca 20640 ggaaggatgt taacatggtt ctgcagagct ccctaggaaa tgacctaagg gttgacggag 20700 ccagcattaa gtttgatagc atttgccttt acgccacctt cttccccatg gcccacaaca 20760 ccgcctccac gcttgaggcc atgcttagaa acgacaccaa cgaccagtcc tttaacgact 20820 atctctccgc cgccaacatg ctctacccta tacccgccaa cgctaccaac gtgcccatat 20880 ccatcccctc ccgcaactgg gcggctttcc gcggctgggc cttcacgcgc cttaagacta 20940 aggaaacccc atcactgggc tcgggctacg acccttatta cacctactct ggctctatac 21000 cctacctaga tggaaccttt tacctcaacc acacctttaa gaaggtggcc attacctttg 21060 actcttctgt cagctggcct ggcaatgacc gcctgcttac ccccaacgag tttgaaatta 21120 agcgctcagt tgacggggag ggttacaacg ttgcccagtg taacatgacc aaagactggt 21180 tcctggtaca aatgctagct aactacaaca ttggctacca gggcttctat atcccagaga 21240 gctacaagga ccgcatgtac tccttcttta gaaacttcca gcccatgagc cgtcaggtgg 21300 tggatgatac taaatacaag gactaccaac aggtgggcat cctacaccaa cacaacaact 21360 ctggatttgt tggctacctt gcccccacca tgcgcgaagg acaggcctac cctgctaact 21420 tcccctatcc gcttataggc aagaccgcag ttgacagcat tacccagaaa aagtttcttt 21480 gcgatcgcac cctttggcgc atcccattct ccagtaactt tatgtccatg ggcgcactca 21540 cagacctggg ccaaaacctt ctctacgcca actccgccca cgcgctagac atgacttttg 21600 aggtggatcc catggacgag cccacccttc tttatgtttt gtttgaagtc tttgacgtgg 21660 tccgtgtgca ccggccgcac cgcggcgtca tcgaaaccgt gtacctgcgc acgcccttct 21720 cggccggcaa cgccacaaca taaagaagca agcaacatca acaacagctg ccgccatggg 21780 ctccagtgag caggaactga aagccattgt caaagatctt ggttgtgggc catatttttt 21840 gggcacctat gacaagcgct ttccaggctt tgtttctcca cacaagctcg cctgcgccat 21900 agtcaatacg gccggtcgcg agactggggg cgtacactgg atggcctttg cctggaaccc 21960 gcactcaaaa acatgctacc tctttgagcc ctttggcttt tctgaccagc gactcaagca 22020 ggtttaccag tttgagtacg agtcactcct gcgccgtagc gccattgctt cttcccccga 22080 ccgctgtata acgctggaaa agtccaccca aagcgtacag gggcccaact cggccgcctg 22140 tggactattc tgctgcatgt ttctccacgc ctttgccaac tggccccaaa ctcccatgga 22200 tcacaacccc accatgaacc ttattaccgg ggtacccaac tccatgctca acagtcccca 22260 ggtacagccc accctgcgtc gcaaccagga acagctctac agcttcctgg agcgccactc 22320 gccctacttc cgcagccaca gtgcgcagat taggagcgcc acttcttttt gtcacttgaa 22380 aaacatgtaa aaataatgta ctagagacac tttcaataaa ggcaaatgct tttatttgta 22440 cactctcggg tgattattta cccccaccct tgccgtctgc gccgtttaaa aatcaaaggg 22500 gttctgccgc gcatcgctat gcgccactgg cagggacacg ttgcgatact ggtgtttagt 22560 gctccactta aactcaggca caaccatccg cggcagctcg gtgaagtttt cactccacag 22620 gctgcgcacc atcaccaacg cgtttagcag gtcgggcgcc gatatcttga agtcgcagtt 22680 ggggcctccg ccctgcgcgc gcgagttgcg atacacaggg ttgcagcact ggaacactat 22740 cagcgccggg tggtgcacgc tggccagcac gctcttgtcg gagatcagat ccgcgtccag 22800 gtcctccgcg ttgctcaggg cgaacggagt caactttggt agctgccttc ccaaaaaggg 22860 cgcgtgccca ggctttgagt tgcactcgca ccgtagtggc atcaaaaggt gaccgtgccc 22920 ggtctgggcg ttaggataca gcgcctgcat aaaagccttg atctgcttaa aagccacctg 22980 agcctttgcg ccttcagaga agaacatgcc gcaagacttg ccggaaaact gattggccgg 23040 acaggccgcg tcgtgcacgc agcaccttgc gtcggtgttg gagatctgca ccacatttcg 23100 gccccaccgg ttcttcacga tcttggcctt gctagactgc tccttcagcg cgcgctgccc 23160 gttttcgctc gtcacatcca tttcaatcac gtgctcctta tttatcataa tgcttccgtg 23220 tagacactta agctcgcctt cgatctcagc gcagcggtgc agccacaacg cgcagcccgt 23280 gggctcgtga tgcttgtagg tcacctctgc aaacgactgc aggtacgcct gcaggaatcg 23340 ccccatcatc gtcacaaagg tcttgttgct ggtgaaggtc agctgcaacc cgcggtgctc 23400 ctcgttcagc caggtcttgc atacggccgc cagagcttcc acttggtcag gcagtagttt 23460 gaagttcgcc tttagatcgt tatccacgtg gtacttgtcc atcagcgcgc gcgcagcctc 23520 catgcccttc tcccacgcag acacgatcgg cacactcagc gggttcatca ccgtaatttc 23580 actttccgct tcgctgggct cttcctcttc ctcttgcgtc cgcataccac gcgccactgg 23640 gtcgtcttca ttcagccgcc gcactgtgcg cttacctcct ttgccatgct tgattagcac 23700 cggtgggttg ctgaaaccca ccatttgtag cgccacatct tctctttctt cctcgctgtc 23760 cacgattacc tctggtgatg gcgggcgctc gggcttggga gaagggcgct tctttttctt 23820 cttgggcgca atggccaaat ccgccgccga ggtcgatggc cgcgggctgg gtgtgcgcgg 23880 caccagcgcg tcttgtgatg agtcttcctc gtcctcggac tcgatacgcc gcctcatccg 23940 cttttttggg ggcgcccggg gaggcggcgg cgacggggac ggggacgaca cgtcctccat 24000 ggttggggga cgtcgcgccg caccgcgtcc gcgctcgggg gtggtttcgc gctgctcctc 24060 ttcccgactg gccatttcct tctcctatag gcagaaaaag atcatggagt cagtcgagaa 24120 gaaggacagc ctaaccgccc cctctgagtt cgccaccacc gcctccaccg atgccgccaa 24180 cgcgcctacc accttccccg tcgaggcacc cccgcttgag gaggaggaag tgattatcga 24240 gcaggaccca ggttttgtaa gcgaagacga cgaggaccgc tcagtaccaa cagaggataa 24300 aaagcaagac caggacaacg cagaggcaaa cgaggaacaa gtcgggcggg gggacgaaag 24360 gcatggcgac tacctagatg tgggagacga cgtgctgttg aagcatctgc agcgccagtg 24420 cgccattatc tgcgacgcgt tgcaagagcg cagcgatgtg cccctcgcca tagcggatgt 24480 cagccttgcc tacgaacgcc acctattctc accgcgcgta ccccccaaac gccaagaaaa 24540 cggcacatgc gagcccaacc cgcgcctcaa cttctacccc gtatttgccg tgccagaggt 24600 gcttgccacc tatcacatct ttttccaaaa ctgcaagata cccctatcct gccgtgccaa 24660 ccgcagccga gcggacaagc agctggcctt gcggcagggc gctgtcatac ctgatatcgc 24720 ctcgctcaac gaagtgccaa aaatctttga gggtcttgga cgcgacgaga agcgcgcggc 24780 aaacgctctg caacaggaaa acagcgaaaa tgaaagtcac tctggagtgt tggtggaact 24840 cgagggtgac aacgcgcgcc tagccgtact aaaacgcagc atcgaggtca cccactttgc 24900 ctacccggca cttaacctac cccccaaggt catgagcaca gtcatgagtg agctgatcgt 24960 gcgccgtgcg cagcccctgg agagggatgc aaatttgcaa gaacaaacag aggagggcct 25020 acccgcagtt ggcgacgagc agctagcgcg ctggcttcaa acgcgcgagc ctgccgactt 25080 ggaggagcga cgcaaactaa tgatggccgc agtgctcgtt accgtggagc ttgagtgcat 25140 gcagcggttc tttgctgacc cggagatgca gcgcaagcta gaggaaacat tgcactacac 25200 ctttcgacag ggctacgtac gccaggcctg caagatctcc aacgtggagc tctgcaacct 25260 ggtctcctac cttggaattt tgcacgaaaa ccgccttggg caaaacgtgc ttcattccac 25320 gctcaagggc gaggcgcgcc gcgactacgt ccgcgactgc gtttacttat ttctatgcta 25380 cacctggcag acggccatgg gcgtttggca gcagtgcttg gaggagtgca acctcaagga 25440 gctgcagaaa ctgctaaagc aaaacttgaa ggacctatgg acggccttca acgagcgctc 25500 cgtggccgcg cacctggcgg acatcatttt ccccgaacgc ctgcttaaaa ccctgcaaca 25560 gggtctgcca gacttcacca gtcaaagcat gttgcagaac tttaggaact ttatcctaga 25620 gcgctcagga atcttgcccg ccacctgctg tgcacttcct agcgactttg tgcccattaa 25680 gtaccgcgaa tgccctccgc cgctttgggg ccactgctac cttctgcagc tagccaacta 25740 ccttgcctac cactctgaca taatggaaga cgtgagcggt gacggtctac tggagtgtca 25800 ctgtcgctgc aacctatgca ccccgcaccg ctccctggtt tgcaattcgc agctgcttaa 25860 cgaaagtcaa attatcggta cctttgagct gcagggtccc tcgcctgacg aaaagtccgc 25920 ggctccgggg ttgaaactca ctccggggct gtggacgtcg gcttaccttc gcaaatttgt 25980 acctgaggac taccacgccc acgagattag gttctacgaa gaccaatccc gcccgccaaa 26040 tgcggagctt accgcctgcg tcattaccca gggccacatt cttggccaat tgcaagccat 26100 caacaaagcc cgccaagagt ttctgctacg aaagggacgg ggggtttact tggaccccca 26160 gtccggcgag gagctcaacc caatcccccc gccgccgcag ccctatcagc agcagccgcg 26220 ggcccttgct tcccaggatg gcacccaaaa agaagctgca gctgccgccg ccacccacgg 26280 acgaggagga atactgggac agtcaggcag aggaggtttt ggacgaggag gaggaggaca 26340 tgatggaaga ctgggagagc ctagacgagg aagcttccga ggtcgaagag gtgtcagacg 26400 aaacaccgtc accctcggtc gcattcccct cgccggcgcc ccagaaatcg gcaaccggtt 26460 ccagcatggc tacaacctcc gctcctcagg cgccgccggc actgcccgtt cgccgaccca 26520 accgtagatg ggacaccact ggaaccaggg ccggtaagtc caagcagccg ccgccgttag 26580 cccaagagca acaacagcgc caaggctacc gctcatggcg cgggcacaag aacgccatag 26640 ttgcttgctt gcaagactgt gggggcaaca tctccttcgc ccgccgcttt cttctctacc 26700 atcacggcgt ggccttcccc cgtaacatcc tgcattacta ccgtcatctc tacagcccat 26760 actgcaccgg cggcagcggc agcggcagca acagcagcgg ccacacagaa gcaaaggcga 26820 ccggatagca agactctgac aaagcccaag aaatccacag cggcggcagc agcaggagga 26880 ggagcgctgc gtctggcgcc caacgaaccc gtatcgaccc gcgagcttag aaacaggatt 26940 tttcccactc tgtatgctat atttcaacag agcaggggcc aagaacaaga gctgaaaata 27000 aaaaacaggt ctctgcgatc cctcacccgc agctgcctgt atcacaaaag cgaagatcag 27060 cttcggcgca cgctggaaga cgcggaggct ctcttcagta aatactgcgc gctgactctt 27120 aaggactagt ttcgcgccct ttctcaaatt taagcgcgaa aactacgtca tctccagcgg 27180 ccacacccgg cgccagcacc tgtcgtcagc gccatttcaa ctttgtatac aaaagttgtg 27240 atgagcaagg aaattcccac gccctacatg tggagttacc agccacaaat gggacttgcg 27300 gctggagctg cccaagacta ctcaacccga ataaactaca tgagcgcggg accccacatg 27360 atatcccggg tcaacggaat acgcgcccac cgaaaccgaa ttctcctgga acaggcggct 27420 attaccacca cacctcgtaa taaccttaat ccccgtagtt ggcccgctgc cctggtgtac 27480 caggaaagtc ccgctcccac cactgtggta cttcccagag acgcccaggc cgaagttcag 27540 atgactaact caggggcgca gcttgcgggc ggctttcgtc acagggtgcg gtcgcccggg 27600 cagggtataa ctcacctgac aatcagaggg cgaggtattc agctcaacga cgagtcggtg 27660 agctcctcgc ttggtctccg tccggacggg acatttcaga tcggcggcgc cggccgctct 27720 tcattcacgc ctcgtcaggc aatcctaact ctgcagacct cgtcctctga gccgcgctct 27780 ggaggcattg gaactctgca atttattgag gagtttgtgc catcggtcta ctttaacccc 27840 ttctcgggac ctcccggcca ctatccggat caatttattc ctaactttga cgcggtaaag 27900 gactcggcgg acggctacga ctgaatgtta agtggagagg cagagcaact gcgcctgaaa 27960 cacctggtcc actgtcgccg ccacaagtgc tttgcccgcg actccggtga gttttgctac 28020 tttgaattgc ccgaggatca tatcgagggc ccggcgcacg gcgtccggct taccgcccag 28080 ggagagcttg cccgtagcct gattcgggag tttacccagc gccccctgct agttgagcgg 28140 gacaggggac cctgtgttct cactgtgatt tgcaactgtc ctaaccctgg attacatcaa 28200 gatctttgtt gccatctctg tgctgagtat aataaataca gaaattaaaa tatactgggg 28260 ctcctatcgc catcctgtaa acgccaccgt cttcacccgc ccaagcaaac caaggcgaac 28320 cttacctggt acttttaaca tctctccctc tgtgatttac aacagtttca acccagacgg 28380 agtgagtcta cgagagaacc tctccgagct cagctactcc atcagaaaaa acaccaccct 28440 ccttacctgc cgggaacgta cgagtgcgtc accggccgct gcaccacacc taccgcctga 28500 ccgtaaacca gactttttcc ggacagacct caataactct gtttaccaga acaggaggtg 28560 agcttagaaa acccttaggg tattaggcca aaggcgcagc tactgtgggg tttatgaaca 28620 attcaagcaa ctctacgggc tattctaatt caggtttctc tagaatcggg gttggggtta 28680 ttctctgtct tgtgattctc tttattctta tactaacgct tctctgccta aggctcgccg 28740 cctgctgtgt gcacatttgc atttattgtc agctttttaa acgctggggt cgccacccaa 28800 gatgattagg tacataatcc taggtttact cacccttgcg tcagcccacg gtaccaccca 28860 aaaggtggat tttaaggagc cagcctgtaa tgttacattc gcagctgaag ctaatgagtg 28920 caccactctt ataaaatgca ccacagaaca tgaaaagctg cttattcgcc acaaaaacaa 28980 aattggcaag tatgctgttt atgctatttg gcagccaggt gacactacag agtataatgt 29040 tacagttttc cagggtaaaa gtcataaaac ttttatgtat acttttccat tttatgaaat 29100 gtgcgacatt accatgtaca tgagcaaaca gtataagttg tggcccccac aaaattgtgt 29160 ggaaaacact ggcactttct gctgcactgc tatgctaatt acagtgctcg ctttggtctg 29220 taccctactc tatattaaat acaaaagcag acgcagcttt attgaggaaa agaaaatgcc 29280 ttaatttact aagttacaaa gctaatgtca ccactaactg ctttactcgc tgcttgcaaa 29340 acaaattcaa aaagttagca ttataattag aataggattt aaaccccccg gtcatttcct 29400 gctcaatacc attcccctga acaattgact ctatgtggga tatgctccag cgctacaacc 29460 ttgaagtcag gcttcctgga tgtcagcatc tgactttggc cagcacctgt cccgcggatt 29520 tgttccagtc caactacagc gacccaccct aacagagatg accaacacaa ccaacgcggc 29580 cgccgctacc ggacttacat ctaccacaaa tacaccccaa gtttctgcct ttgtcaataa 29640 ctgggataac ttgggcatgt ggtggttctc catagcgctt atgtttgtat gccttattat 29700 tatgtggctc atctgctgcc taaagcgcaa acgcgcccga ccacccatct atagtcccat 29760 cattgtgcta cacccaaaca atgatggaat ccatagattg gacggactga aacacatgtt 29820 cttttctctt acagtatgat taaatgagac atgattcctc gagtttttat attactgacc 29880 cttgttgcgc ttttttgtgc gtgctccaca ttggctgcgg tttctcacat cgaagtagac 29940 tgcattccag ccttcacagt ctatttgctt tacggatttg tcaccctcac gctcatctgc 30000 agcctcatca ctgtggtcat cgcctttatc cagtgcattg actgggtctg tgtgcgcttt 30060 gcatatctca gacaccatcc ccagtacagg gacaggacta tagctgagct tcttagaatt 30120 ctttaattat gaaatttact gtgacttttc tgctgattat ttgcacccta tctgcgtttt 30180 gttccccgac ctccaagcct caaagacata tatcatgcag attcactcgt atatggaata 30240 ttccaagttg ctacaatgaa aaaagcgatc tttccgaagc ctggttatat gcaatcatct 30300 ctgttatggt gttctgcagt accatcttag ccctagctat atatccctac cttgacattg 30360 gctggaacgc aatagatgcc atgaaccacc caactttccc cgcgcccgct atgcttccac 30420 tgcaacaagt tgttgccggc ggctttgtcc cagccaatca gcctcgccca ccttctccca 30480 cccccactga aatcagctac tttaatctaa caggaggaga tgactgacac cctagatcta 30540 gaaatggacg gaattattac agagcagcgc ctgctagaaa gacgcagggc agcggccgag 30600 caacagcgca tgaatcaaga gctccaagac atggttaact tgcaccagtg caaaaggggt 30660 atcttttgtc tggtaaagca ggccaaagtc acctacgaca gtaataccac cggacaccgc 30720 cttagctaca agttgccaac caagcgtcag aaattggtgg tcatggtggg agaaaagccc 30780 attaccataa ctcagcactc ggtagaaacc gaaggctgca ttcactcacc ttgtcaagga 30840 cctgaggatc tctgcaccct tattaagacc ctgtgcggtc tcaaagatct tattcccttt 30900 aactaataaa aaaaaataat aaagcatcac ttacttaaaa tcagttagca aatttctgtc 30960 cagtttattc agcagcacct ccttgccctc ctcccagctc tggtattgca gcttcctcct 31020 ggctgcaaac tttctccaca atctaaatgg aatgtcagtt tcctcctgtt cctgtccatc 31080 cgcacccact atcttcatgt tgttgcagat ggtgagcaaa ggcgaagagc tgttcaccgg 31140 cgtggtgccc atcctggtgg agctggacgg cgacgtgaac ggccacaagt tcagcgtgag 31200 cggcgagggc gagggcgacg ccacctacgg caagctgacc ctgaagctgc tgtgcaccac 31260 cggcaagctg cccgtgccct ggcccaccct ggtgaccacc ctgggctacg gcgtgcagtg 31320 cttcgcccgg taccccgacc acatgaagca gcacgacttc ttcaagagcg ccatgcccga 31380 gggctacgtg caggagcgga ccatcttctt caaggacgac ggcaactaca agacccgggc 31440 cgaggtgaag ttcgagggcg acaccctggt gaaccggatc gagctgaagg gcatcgactt 31500 caaggaggac ggcaacatcc tgggccacaa gctggagtac aactacaaca gccacaacgt 31560 gtacatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccggcacaa 31620 catcgaggac ggcggcgtgc agctggccga ccactaccag cagaacaccc ccatcggcga 31680 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagagcgccc tgttcaagga 31740 ccccaacgag aagcgggacc acatggtgct gctggagttc ctgaccgccg ccggcatcac 31800 cgagggcatg aacgagctct ataagggaag cggagctact aacttcagcc tgctgaagca 31860 ggctggtgac gtcgaggaga atcctggccc aatgaagcgc gcaagaccgt ctgaagatac 31920 cttcaacccc gtgtatccat atgacacgga aaccggtcct ccaactgtgc cttttcttac 31980 tcctcccttt gtatccccca atgggtttca agagagtccc cctggggtac tctctttgcg 32040 cctatccgaa cctctagtta cctccaatgg catgcttgcg ctcaaaatgg gcaacggcct 32100 ctctctggac gaggccggca accttacctc ccaaaatgta accactgtga gcccacctct 32160 caaaaaaacc aagtcaaaca taaacctgga aatatctgca cccctcacag ttacctcaga 32220 agccctaact gtggctgccg ccgcacctct aatggtcgcg ggcaacacac tcaccatgca 32280 atcacaggcc ccgctaaccg tgcacgactc caaacttagc attgccaccc aaggacccct 32340 cacagtgtca gaaggaaagc tagccctgca aacatcaggc cccctcacca ccaccgatag 32400 cagtaccctt actatcactg cctcaccccc tctaactact gccactggta gcttgggcat 32460 tgacttgaaa gagcccattt atacacaaaa tggaaaacta ggactaaagt acggggctcc 32520 tttgcatgta acagacgacc taaacacttt gaccgtagca actggtccag gtgtgactat 32580 taataatact tccttgcaaa ctaaagttac tggagccttg ggttttgatt cacaaggcaa 32640 tatgcaactt aatgtagcag gaggactaag gattgattct caaaacagac gccttatact 32700 tgatgttagt tatccgtttg atgctcaaaa ccaactaaat ctaagactag gacagggccc 32760 tctttttata aactcagccc acaacttgga tattaactac aacaaaggcc tttacttgtt 32820 tacagcttca aacaattcca aaaagcttga ggttaaccta agcactgcca aggggttgat 32880 gtttgacgct acagccatag ccattaatgc aggagatggg cttgaatttg gttcacctaa 32940 tgcaccaaac acaaatcccc tcaaaacaaa aattggccat ggcctagaat ttgattcaaa 33000 caaggctatg gttcctaaac taggaactgg ccttagtttt gacagcacag gtgccattac 33060 agtaggaaac aaaaataatg ataagctaac tttgtggacc acaccagctc catctcctaa 33120 ctgtagacta aatgcagaga aagatgctaa actcactttg gtcttaacaa aatgtggcag 33180 tcaaatactt gctacagttt cagttttggc tgttaaaggc agtttggctc caatatctgg 33240 aacagttcaa agtgctcatc ttattataag atttgacgaa aatggagtgc tactaaacaa 33300 ttccttcctg gacccagaat attggaactt tagaaatgga gatcttactg aaggcacagc 33360 ctatacaaac gctgttggat ttatgcctaa cctatcagct tatccaaaat ctcacggtaa 33420 aactgccaaa agtaacattg tcagtcaagt ttacttaaac ggagacaaaa ctaaacctgt 33480 aacactaacc attacactaa acggtacaca ggaaacagga gacacaactc caagtgcata 33540 ctctatgtca ttttcatggg actggtctgg ccacaactac attaatgaaa tatttgccac 33600 atcctcttac actttttcat acattgccca agaataaaga atcgtttgtg ttatgtttca 33660 acgtgtttat ttttcaattg cagaaaattt cgaatcattt ttcattcagt agtatagccc 33720 caccaccaca tagcttatac agatcaccgt acctctttat ttttcaattg cagaaaattt 33780 caagtcattt ttcattcagt agtatagccc caccaccaca tagcttatac agatcaccgt 33840 acctcaactt tgtataataa agttgtaatc aaactcacag aaccctagta ttcaacctgc 33900 cacctccctc ccaacacaca gagtacacag tcctttctcc ccggctggcc ttaaaaagca 33960 tcatatcatg ggtaacagac atattcttag gtgttatatt ccacacggtt tcctgtcgag 34020 ccaaacgctc atcagtgata ttaataaact ccccgggcag ctcacttaag ttcatgtcgc 34080 tgtccagctg ctgagccaca ggctgctgtc caacttgcgg ttgcttaacg ggcggcgaag 34140 gagaagtcca cgcctacatg ggggtagagt cataatcgtg catcaggata gggcggtggt 34200 gctgcagcag cgcgcgaata aactgctgcc gccgccgctc cgtcctgcag gaatacaaca 34260 tggcagtggt ctcctcagcg atgattcgca ccgcccgcag cataaggcgc cttgtcctcc 34320 gggcacagca gcgcaccctg atctcactta aatcagcaca gtaactgcag cacagcacca 34380 caatattgtt caaaatccca cagtgcaagg cgctgtatcc aaagctcatg gcggggacca 34440 cagaacccac gtggccatca taccacaagc gcaggtagat taagtggcga cccctcataa 34500 acacgctgga cataaacatt acctcttttg gcatgttgta attcaccacc tcccggtacc 34560 atataaacct ctgattaaac atggcgccat ccaccaccat cctaaaccag ctggccaaaa 34620 cctgcccgcc ggctatacac tgcagggaac cgggactgga acaatgacag tggagagccc 34680 aggactcgta accatggatc atcatgctcg tcatgatatc aatgttggca caacacaggc 34740 acacgtgcat acacttcctc aggattacaa gctcctcccg cgttagaacc atatcccagg 34800 gaacaaccca ttcctgaatc agcgtaaatc ccacactgca gggaagacct cgcacgtaac 34860 tcacgttgtg cattgtcaaa gtgttacatt cgggcagcag cggatgatcc tccagtatgg 34920 tagcgcgggt ttctgtctca aaaggaggta gacgatccct actgtacgga gtgcgccgag 34980 acaaccgaga tcgtgttggt cgtagtgtca tgccaaatgg aacgccggac gtagtcatat 35040 ttcctgaagc aaaaccaggt gcgggcgtga caaacagatc tgcgtctccg gtctcgccgc 35100 ttagatcgct ctgtgtagta gttgtagtat atccactctc tcaaagcatc caggcgcccc 35160 ctggcttcgg gttctatgta aactccttca tgcgccgctg ccctgataac atccaccacc 35220 gcagaataag ccacacccag ccaacctaca cattcgttct gcgagtcaca cacgggagga 35280 gcgggaagag ctggaagaac catgtttttt tttttattcc aaaagattat ccaaaacctc 35340 aaaatgaaga tctattaagt gaacgcgctc ccctccggtg gcgtggtcaa actctacagc 35400 caaagaacag ataatggcat ttgtaagatg ttgcacaatg gcttccaaaa ggcaaacggc 35460 cctcacgtcc aagtggacgt aaaggctaaa cccttcaggg tgaatctcct ctataaacat 35520 tccagcacct tcaaccatgc ccaaataatt ctcatctcgc caccttctca atatatctct 35580 aagcaaatcc cgaatattaa gtccggccat tgtaaaaatc tgctccagag cgccctccac 35640 cttcagcctc aagcagcgaa tcatgattgc aaaaattcag gttcctcaca gacctgtata 35700 agattcaaaa gcggaacatt aacaaaaata ccgcgatccc gtaggtccct tcgcagggcc 35760 agctgaacat aatcgtgcag gtctgcacgg accagcgcgg ccacttcccc gccaggaacc 35820 atgacaaaag aacccacact gattatgaca cgcatactcg gagctatgct aaccagcgta 35880 gccccgatgt aagcttgttg catgggcggc gatataaaat gcaaggtgct gctcaaaaaa 35940 tcaggcaaag cctcgcgcaa aaaagaaagc acatcgtagt catgctcatg cagataaagg 36000 caggtaagct ccggaaccac cacagaaaaa gacaccattt ttctctcaaa catgtctgcg 36060 ggtttctgca taaacacaaa ataaaataac aaaaaaacat ttaaacatta gaagcctgtc 36120 ttacaacagg aaaaacaacc cttataagca taagacggac tacggccatg ccggcgtgac 36180 cgtaaaaaaa ctggtcaccg tgattaaaaa gcaccaccga cagctcctcg gtcatgtccg 36240 gagtcataat gtaagactcg gtaaacacat caggttgatt cacatcggtc agtgctaaaa 36300 agcgaccgaa atagcccggg ggaatacata cccgcaggcg tagagacaac attacagccc 36360 ccataggagg tataacaaaa ttaataggag agaaaaacac ataaacacct gaaaaaccct 36420 cctgcctagg caaaatagca ccctcccgct ccagaacaac atacagcgct tccacagcgg 36480 cagccataac agtcagcctt accagtaaaa aagaaaacct attaaaaaaa caccactcga 36540 cacggcacca gctcaatcag tcacagtgta aaaaagggcc aagtgcagag cgagtatata 36600 taggactaaa aaatgacgta acggttaaag tccacaaaaa acacccagaa aaccgcacgc 36660 gaacctacgc ccagaaacga aagccaaaaa acccacaact tcctcaaatc gtcacttccg 36720 ttttcccacg ttacgtcact tcccatttta agaaaactac aattcccaac acatacaagt 36780 tactccgccc taaaacctac gtcacccgcc ccgttcccac gccccgcgcc acgtcacaaa 36840 ctccaccccc tcattatcat attggcttca atccaaaata aggtatatta ttgatgatg 36899 <210> 13 <211> 36794 <212> DNA <213> Artificial Sequence <220> <223> Synthetic adenovirus genome Fiber-P2A-YPet <400> 13 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt 60 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt 120 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg 180 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag 240 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga 300 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg 360 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc 420 cgggtcaaag ttggcgtttt attattatag tcagctgacg tgtagtgtat ttatacccgg 480 tgagttcctc aagaggccac tcttgagtgc cagcgagtag agttttctcc tccgagccgc 540 tccgacaccg ggactgaaaa tgagacatat tatctgccac ggaggtgtta ttaccgaaga 600 aatggccgcc agtcttttgg accagctgat cgaagaggta ctggctgata atcttccacc 660 tcctagccat tttgaaccac ctacccttca cgaactgtat gatttagacg tgacggcccc 720 cgaagatccc aacgaggagg cggtttcgca gatttttccc gactctgtaa tgttggcggt 780 gcaggaaggg attgacttac tcacttttcc gccggcgccc ggttctccgg agccgcctca 840 cctttcccgg cagcccgagc agccggagca gagagccttg ggtccggttt ctatgccaaa 900 ccttgtaccg gaggtgatcg atcttacctg ccacgaggct ggctttccac ccagtgacga 960 cgaggatgaa gagggtgagg agtttgtgtt agattatgtg gagcaccccg ggcacggttg 1020 caggtcttgt cattatcacc ggaggaatac gggggaccca gatattatgt gttcgctttg 1080 ctatatgagg acctgtggca tgtttgtcta cagtaagtga aaattatggg cagtgggtga 1140 tagagtggtg ggtttggtgt ggtaattttt tttttaattt ttacagtttt gtggtttaaa 1200 gaattttgta ttgtgatttt tttaaaaggt cctgtgtctg aacctgagcc tgagcccgag 1260 ccagaaccgg agcctgcaag acctacccgc cgtcctaaaa tggcgcctgc tatcctgaga 1320 cgcccgacat cacctgtgtc tagagaatgc aatagtagta cggatagctg tgactccggt 1380 ccttctaaca cacctcctga gatacacccg gtggtcccgc tgtgccccat taaaccagtt 1440 gccgtgagag ttggtgggcg tcgccaggct gtggaatgta tcgaggactt gcttaacgag 1500 cctgggcaac ctttggactt gagctgtaaa cgccccaggc cataaggtgt aaacctgtga 1560 ttgcgtgtgt ggttaacgcc tttgtttgct gaatgagttg atgtaagttt aataaagggt 1620 gagataatgt ttaacttgca tggcgtgtta aatggggcgg ggcttaaagg gtatataatg 1680 cgccgtgggc taatcttggt tacatctgac ctcatggagg cttgggagtg tttggaagat 1740 ttttctgctg tgcgtaactt gctggaacag agctctaaca gtacctcttg gttttggagg 1800 tttctgtggg gctcatccca ggcaaagtta gtctgcagaa ttaaggagga ttacaagtgg 1860 gaatttgaag agcttttgaa atcctgtggt gagctgtttg attctttgaa tctgggtcac 1920 caggcgcttt tccaagagaa ggtcatcaag actttggatt tttccacacc ggggcgcgct 1980 gcggctgctg ttgctttttt gagttttata aaggataaat ggagcgaaga aacccatctg 2040 agcggggggt acctgctgga ttttctggcc atgcatctgt ggagagcggt tgtgagacac 2100 aagaatcgcc tgctactgtt gtcttccgtc cgcccggcga taataccgac ggaggagcag 2160 cagcagcagc aggaggaagc caggcggcgg cggcaggagc agagcccatg gaacccgaga 2220 gccggcctgg accctcggga atgaatgttg tacaggtggc tgaactgtat ccagaactga 2280 gacgcatttt gacaattaca gaggatgggc aggggctaaa gggggtaaag agggagcggg 2340 gggcttgtga ggctacagag gaggctagga atctagcttt tagcttaatg accagacacc 2400 gtcctgagtg tattactttt caacagatca aggataattg cgctaatgag cttgatctgc 2460 tggcgcagaa gtattccata gagcagctga ccacttactg gctgcagcca ggggatgatt 2520 ttgaggaggc tattagggta tatgcaaagg tggcacttag gccagattgc aagtacaaga 2580 tcagcaaact tgtaaatatc aggaattgtt gctacatttc tgggaacggg gccgaggtgg 2640 agatagatac ggaggatagg gtggccttta gatgtagcat gataaatatg tggccggggg 2700 tgcttggcat ggacggggtg gttattatga atgtaaggtt tactggcccc aattttagcg 2760 gtacggtttt cctggccaat accaacctta tcctacacgg tgtaagcttc tatgggttta 2820 acaatacctg tgtggaagcc tggaccgatg taagggttcg gggctgtgcc ttttactgct 2880 gctggaaggg ggtggtgtgt cgccccaaaa gcagggcttc aattaagaaa tgcctctttg 2940 aaaggtgtac cttgggtatc ctgtctgagg gtaactccag ggtgcgccac aatgtggcct 3000 ccgactgtgg ttgcttcatg ctagtgaaaa gcgtggctgt gattaagcat aacatggtat 3060 gtggcaactg cgaggacagg gcctctcaga tgctgacctg ctcggacggc aactgtcacc 3120 tgctgaagac cattcacgta gccagccact ctcgcaaggc ctggccagtg tttgagcata 3180 acatactgac ccgctgttcc ttgcatttgg gtaacaggag gggggtgttc ctaccttacc 3240 aatgcaattt gagtcacact aagatattgc ttgagcccga gagcatgtcc aaggtgaacc 3300 tgaacggggt gtttgacatg accatgaaga tctggaaggt gctgaggtac gatgagaccc 3360 gcaccaggtg cagaccctgc gagtgtggcg gtaaacatat taggaaccag cctgtgatgc 3420 tggatgtgac cgaggagctg aggcccgatc acttggtgct ggcctgcacc cgcgctgagt 3480 ttggctctag cgatgaagat acagattgag gtactgaaat gtgtgggcgt ggcttaaggg 3540 tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg 3600 ccgccgccca acttttctat acaaagttgt aatgagcacc aactcgtttg atggaagcat 3660 tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat 3720 gggctccagc attgatggtc gccccgtcct gcccgcaaac tctactacct tgacctacga 3780 gaccgtgtct ggaacgccgt tggagactgc agcctccgcc gccgcttcag ccgctgcagc 3840 caccgcccgc gggattgtga ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc 3900 ttcccgttca tccgcccgcg atgacaagtt gacggctctt ttggcacaat tggattcttt 3960 gacccgggaa cttaatgtcg tttctcagca gctgttggat ctgcgccagc aggtttctgc 4020 cctgaaggct tcctcccctc ccaatgcggt ttaaaacata aataaaaaac cagactctgt 4080 ttggatttgg atcaagcata agtgtcttgc tgtctttatt taggggtttt gcgcgcgcgg 4140 taggcccggg accagcggtc tcggtcgttg agggtcctgt gtattttttc caggacgtgg 4200 taaaggtgac tctggatgtt cagatacatg ggcataagcc cgtctctggg gtggaggtag 4260 caccactgca gagcttcatg ctgcggggtg gtgttgtaga tgatccagtc gtagcaggag 4320 cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc tgattgccag gggcaggccc 4380 ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt gcatacgtgg ggatatgaga 4440 tgcatcttgg actgtatttt taggttggct atgttcccag ccatatccct ccggggattc 4500 atgttgtgca gaaccaccag cacagtgtat ccggtgcact tgggaaattt gtcatgtagc 4560 ttagaaggaa atgcgtggaa gaacttggag acgcccttgt gacctccaag attttccatg 4620 cattcgtcca taatgatggc aatgggccca cgggcggcgg cctgggcgaa gatatttctg 4680 ggatcactaa cgtcatagtt gtgttccagg atgagatcgt cataggccat ttttacaaag 4740 cgcgggcgga gggtgccaga ctgcggtata atggttccat ccggcccagg ggcgtagtta 4800 ccctcacaga tttgcatttc ccacgctttg agttcagatg gggggatcat gtctacctgc 4860 ggggcgatga agaaaacggt ttccggggta ggggagatca gctgggaaga aagcaggttc 4920 ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa tcacacctat taccgggtgc 4980 aactggtagt taagagagct gcagctgccg tcatccctga gcaggggggc cacttcgtta 5040 agcatgtccc tgactcgcat gttttccctg accaaatccg ccagaaggcg ctcgccgccc 5100 agcgatagca gttcttgcaa ggaagcaaag tttttcaacg gtttgagacc gtccgccgta 5160 ggcatgcttt tgagcgtttg accaagcagt tccaggcggt cccacagctc ggtcacctgc 5220 tctacggcat ctcgatccag catatctcct cgtttcgcgg gttggggcgg ctttcgctgt 5280 acggcagtag tcggtgctcg tccagacggg ccagggtcat gtctttccac gggcgcaggg 5340 tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc tccgggctgc gcgctggcca 5400 gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg ccggtcttcg ccctgcgcgt 5460 cggccaggta gcatttgacc atggtgtcat agtccagccc ctccgcggcg tggcccttgg 5520 cgcgcagctt gcccttggag gaggcgccgc acgaggggca gtgcagactt ttgagggcgt 5580 agagcttggg cgcgagaaat accgattccg gggagtaggc atccgcgccg caggccccgc 5640 agacggtctc gcattccacg agccaggtga gctctggccg ttcggggtca aaaaccaggt 5700 ttcccccatg ctttttgatg cgtttcttac ctctggtttc catgagccgg tgtccacgct 5760 cggtgacgaa aaggctgtcc gtgtccccgt atacagactt gagaggcctg tcctcgagcg 5820 gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacaaag gctcgcgtcc 5880 aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact agggggtcca 5940 ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag gtgattggtt 6000 tgtaggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag ggggtggggg 6060 cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt tggggtgagt 6120 actccctctg aaaagcgggc atgacttctg cgctaagatt gtcagtttcc aaaaacgagg 6180 aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgca tccatctggt 6240 cagaaaagac aatctttttg ttgtcaagct tggtggcaaa cgacccgtag agggcgttgg 6300 acagcaactt ggcgatggag cgcagggttt ggtttttgtc gcgatcggcg cgctccttgg 6360 ccgcgatgtt tagctgcacg tattcgcgcg caacgcaccg ccattcggga aagacggtgg 6420 tgcgctcgtc gggcaccagg tgcacgcgcc aaccgcggtt gtgcagggtg acaaggtcaa 6480 cgctggtggc tacctctccg cgtaggcgct cgttggtcca gcagaggcgg ccgcccttgc 6540 gcgagcagaa tggcggtagg gggtctagct gcgtctcgtc cggggggtct gcgtccacgg 6600 taaagacccc gggcagcagg cgcgcgtcga agtagtctat cttgcatcct tgcaagtcta 6660 gcgcctgctg ccatgcgcgg gcggcaagcg cgcgctcgta tgggttgagt gggggacccc 6720 atggcatggg gtgggtgagc gcggaggcgt acatgccgca aatgtcgtaa acgtagaggg 6780 gctctctgag tattccaaga tatgtagggt agcatcttcc accgcggatg ctggcgcgca 6840 cgtaatcgta tagttcgtgc gagggagcga ggaggtcggg accgaggttg ctacgggcgg 6900 gctgctctgc tcggaagact atctgcctga agatggcatg tgagttggat gatatggttg 6960 gacgctggaa gacgttgaag ctggcgtctg tgagacctac cgcgtcacgc acgaaggagg 7020 cgtaggagtc gcgcagcttg ttgaccagct cggcggtgac ctgcacgtct agggcgcagt 7080 agtccagggt ttccttgatg atgtcatact tatcctgtcc cttttttttc cacagctcgc 7140 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct 7200 ccgaacggta agagcctagc atgtagaact ggttgacggc ctggtaggcg cagcatccct 7260 tttctacggg tagcgcgtat gcctgcgcgg ccttccggag cgaggtgtgg gtgagcgcaa 7320 aggtgtccct gaccatgact ttgaggtact ggtatttgaa gtcagtgtcg tcgcatccgc 7380 cctgctccca gagcaaaaag tccgtgcgct ttttggaacg cggatttggc agggcgaagg 7440 tgacatcgtt gaagagtatc tttcccgcgc gaggcataaa gttgcgtgtg atgcggaagg 7500 gtcccggcac ctcggaacgg ttgttaatta cctgggcggc gagcacgatc tcgtcaaagc 7560 cgttgatgtt gtggcccaca atgtaaagtt ccaagaagcg cgggatgccc ttgatggaag 7620 gcaatttttt aagttcctcg taggtgagct cttcagggga gctgagcccg tgctctgaaa 7680 gggcccagtc tgcaagatga gggttggaag cgacgaatga gctccacagg tcacgggcca 7740 ttagcatttg caggtggtcg cgaaaggtcc taaactggcg acctatggcc attttttctg 7800 gggtgatgca gtagaaggta agcgggtctt gttcccagcg gtcccatcca aggttcgcgg 7860 ctaggtctcg cgcggcagtc actagaggct catctccgcc gaacttcatg accagcatga 7920 agggcacgag ctgcttccca aaggccccca tccaagtata ggtctctaca tcgtaggtga 7980 caaagagacg ctcggtgcga ggatgcgagc cgatcgggaa gaactggatc tcccgccacc 8040 aattggagga gtggctattg atgtggtgaa agtagaagtc cctgcgacgg gccgaacact 8100 cgtgctggct tttgtaaaaa cgtgcgcagt actggcagcg gtgcacgggc tgtacatcct 8160 gcacgaggtt gacctgacga ccgcgcacaa ggaagcagag tgggaatttg agcccctcgc 8220 ctggcgggtt tggctggtgg tcttctactt cggctgcttg tccttgaccg tctggctgct 8280 cgaggggagt tacggtggat cggaccacca cgccgcgcga gcccaaagtc cagatgtccg 8340 cgcgcggcgg tcggagcttg atgacaacat cgcgcagatg ggagctgtcc atggtctgga 8400 gctcccgcgg cgtcaggtca ggcgggagct cctgcaggtt tacctcgcat agacgggtca 8460 gggcgcgggc tagatccagg tgatacctaa tttccagggg ctggttggtg gcggcgtcga 8520 tggcttgcaa gaggccgcat ccccgcggcg cgactacggt accgcgcggc gggcggtggg 8580 ccgcgggggt gtccttggat gatgcatcta aaagcggtga cgcgggcgag cccccggagg 8640 tagggggggc tccggacccg ccgggagagg gggcaggggc acgtcggcgc cgcgcgcggg 8700 caggagctgg tgctgcgcgc gtaggttgct ggcgaacgcg acgacgcggc ggttgatctc 8760 ctgaatctgg cgcctctgcg tgaagacgac gggcccggtg agcttgagcc tgaaagagag 8820 ttcgacagaa tcaatttcgg tgtcgttgac ggcggcctgg cgcaaaatct cctgcacgtc 8880 tcctgagttg tcttgatagg cgatctcggc catgaactgc tcgatctctt cctcctggag 8940 atctccgcgt ccggctcgct ccacggtggc ggcgaggtcg ttggaaatgc gggccatgag 9000 ctgcgagaag gcgttgaggc ctccctcgtt ccagacgcgg ctgtagacca cgcccccttc 9060 ggcatcgcgg gcgcgcatga ccacctgcgc gagattgagc tccacgtgcc gggcgaagac 9120 ggcgtagttt cgcaggcgct gaaagaggta gttgagggtg gtggcggtgt gttctgccac 9180 gaagaagtac ataacccagc gtcgcaacgt ggattcgttg atatccccca aggcctcaag 9240 gcgctccatg gcctcgtaga agtccacggc gaagttgaaa aactgggagt tgcgcgccga 9300 cacggttaac tcctcctcca gaagacggat gagctcggcg acagtgtcgc gcacctcgcg 9360 ctcaaaggct acaggggcct cttcttcttc ttcaatctcc tcttccataa gggcctcccc 9420 ttcttcttct tctggcggcg gtgggggagg ggggacacgg cggcgacgac ggcgcaccgg 9480 gaggcggtcg acaaagcgct cgatcatctc cccgcggcga cggcgcatgg tctcggtgac 9540 ggcgcggccg ttctcgcggg ggcgcagttg gaagacgccg cccgtcatgt cccggttatg 9600 ggttggcggg gggctgccat gcggcaggga tacggcgcta acgatgcatc tcaacaattg 9660 ttgtgtaggt actccgccgc cgagggacct gagcgagtcc gcatcgaccg gatcggaaaa 9720 cctctcgaga aaggcgtcta accagtcaca gtcgcaaggt aggctgagca ccgtggcggg 9780 cggcagcggg cggcggtcgg ggttgtttct ggcggaggtg ctgctgatga tgtaattaaa 9840 gtaggcggtc ttgagacggc ggatggtcga cagaagcacc atgtccttgg gtccggcctg 9900 ctgaatgcgc aggcggtcgg ccatgcccca ggcttcgttt tgacatcggc gcaggtcttt 9960 gtagtagtct tgcatgagcc tttctaccgg cacttcttct tctccttcct cttgtcctgc 10020 atctcttgca tctatcgctg cggcggcggc ggagtttggc cgtaggtggc gccctcttcc 10080 tcccatgcgt gtgaccccga agcccctcat cggctgaagc agggctaggt cggcgacaac 10140 gcgctcggct aatatggcct gctgcacctg cgtgagggta gactggaagt catccatgtc 10200 cacaaagcgg tggtatgcgc ccgtgttgat ggtgtaagtg cagttggcca taacggacca 10260 gttaacggtc tggtgacccg gctgcgagag ctcggtgtac ctgagacgcg agtaagccct 10320 cgagtcaaat acgtagtcgt tgcaagtccg caccaggtac tggtatccca ccaaaaagtg 10380 cggcggcggc tggcggtaga ggggccagcg tagggtggcc ggggctccgg gggcgagatc 10440 ttccaacata aggcgatgat atccgtagat gtacctggac atccaggtga tgccggcggc 10500 ggtggtggag gcgcgcggaa agtcgcggac gcggttccag atgttgcgca gcggcaaaaa 10560 gtgctccatg gtcgggacgc tctggccggt caggcgcgcg caatcgttga cgctctagac 10620 cgtgcaaaag gagagcctgt aagcgggcac tcttccgtgg tctggtggat aaattcgcaa 10680 gggtatcatg gcggacgacc ggggttcgag ccccgtatcc ggccgtccgc cgtgatccat 10740 gcggttaccg cccgcgtgtc gaacccaggt gtgcgacgtc agacaacggg ggagtgctcc 10800 ttttggcttc cttccaggcg cggcggctgc tgcgctagct tttttggcca ctggccgcgc 10860 gcagcgtaag cggttaggct ggaaagcgaa agcattaagt ggctcgctcc ctgtagccgg 10920 agggttattt tccaagggtt gagtcgcggg acccccggtt cgagtctcgg accggccgga 10980 ctgcggcgaa cgggggtttg cctccccgtc atgcaagacc ccgcttgcaa attcctccgg 11040 aaacagggac gagccccttt tttgcttttc ccagatgcat ccggtgctgc ggcagatgcg 11100 cccccctcct cagcagcggc aagagcaaga gcagcggcag acatgcaggg caccctcccc 11160 tcctcctacc gcgtcaggag gggcgacatc cgcggttgac gcggcagcag atggtgatta 11220 cgaacccccg cggcgccggg cccggcacta cctggacttg gaggagggcg agggcctggc 11280 gcggctagga gcgccctctc ctgagcggta cccaagggtg cagctgaagc gtgatacgcg 11340 tgaggcgtac gtgccgcggc agaacctgtt tcgcgaccgc gagggagagg agcccgagga 11400 gatgcgggat cgaaagttcc acgcagggcg cgagctgcgg catggcctga atcgcgagcg 11460 gttgctgcgc gaggaggact ttgagcccga cgcgcgaacc gggattagtc ccgcgcgcgc 11520 acacgtggcg gccgccgacc tggtaaccgc atacgagcag acggtgaacc aggagattaa 11580 ctttcaaaaa agctttaaca accacgtgcg tacgcttgtg gcgcgcgagg aggtggctat 11640 aggactgatg catctgtggg actttgtaag cgcgctggag caaaacccaa atagcaagcc 11700 gctcatggcg cagctgttcc ttatagtgca gcacagcagg gacaacgagg cattcaggga 11760 tgcgctgcta aacatagtag agcccgaggg ccgctggctg ctcgatttga taaacatcct 11820 gcagagcata gtggtgcagg agcgcagctt gagcctggct gacaaggtgg ccgccatcaa 11880 ctattccatg cttagcctgg gcaagtttta cgcccgcaag atataccata ccccttacgt 11940 tcccatagac aaggaggtaa agatcgaggg gttctacatg cgcatggcgc tgaaggtgct 12000 taccttgagc gacgacctgg gcgtttatcg caacgagcgc atccacaagg ccgtgagcgt 12060 gagccggcgg cgcgagctca gcgaccgcga gctgatgcac agcctgcaaa gggccctggc 12120 tggcacgggc agcggcgata gagaggccga gtcctacttt gacgcgggcg ctgacctgcg 12180 ctgggcccca agccgacgcg ccctggaggc agctggggcc ggacctgggc tggcggtggc 12240 acccgcgcgc gctggcaacg tcggcggcgt ggaggaatat gacgaggacg atgagtacga 12300 gccagaggac ggcgagtact aagcggtgat gtttctgatc agatgatgca agacgcaacg 12360 gacccggcgg tgcgggcggc gctgcagagc cagccgtccg gccttaactc cacggacgac 12420 tggcgccagg tcatggaccg catcatgtcg ctgactgcgc gcaatcctga cgcgttccgg 12480 cagcagccgc aggccaaccg gctctccgca attctggaag cggtggtccc ggcgcgcgca 12540 aaccccacgc acgagaaggt gctggcgatc gtaaacgcgc tggccgaaaa cagggccatc 12600 cggcccgacg aggccggcct ggtctacgac gcgctgcttc agcgcgtggc tcgttacaac 12660 agcggcaacg tgcagaccaa cctggaccgg ctggtggggg atgtgcgcga ggccgtggcg 12720 cagcgtgagc gcgcgcagca gcagggcaac ctgggctcca tggttgcact aaacgccttc 12780 ctgagtacac agcccgccaa cgtgccgcgg ggacaggagg actacaccaa ctttgtgagc 12840 gcactgcggc taatggtgac tgagacaccg caaagtgagg tgtaccagtc tgggccagac 12900 tattttttcc agaccagtag acaaggcctg cagaccgtaa acctgagcca ggctttcaaa 12960 aacttgcagg ggctgtgggg ggtgcgggct cccacaggcg accgcgcgac cgtgtctagc 13020 ttgctgacgc ccaactcgcg cctgttgctg ctgctaatag cgcccttcac ggacagtggc 13080 agcgtgtccc gggacacata cctaggtcac ttgctgacac tgtaccgcga ggccataggt 13140 caggcgcatg tggacgagca tactttccag gagattacaa gtgtcagccg cgcgctgggg 13200 caggaggaca cgggcagcct ggaggcaacc ctaaactacc tgctgaccaa ccggcggcag 13260 aagatcccct cgttgcacag tttaaacagc gaggaggagc gcattttgcg ctacgtgcag 13320 cagagcgtga gccttaacct gatgcgcgac ggggtaacgc ccagcgtggc gctggacatg 13380 accgcgcgca acatggaacc gggcatgtat gcctcaaacc ggccgtttat caaccgccta 13440 atggactact tgcatcgcgc ggccgccgtg aaccccgagt atttcaccaa tgccatcttg 13500 aacccgcact ggctaccgcc ccctggtttc tacaccgggg gattcgaggt gcccgagggt 13560 aacgatggat tcctctggga cgacatagac gacagcgtgt tttccccgca accgcagacc 13620 ctgctagagt tgcaacagcg cgagcaggca gaggcggcgc tgcgaaagga aagcttccgc 13680 aggccaagca gcttgtccga tctaggcgct gcggccccgc ggtcagatgc tagtagccca 13740 tttccaagct tgatagggtc tcttaccagc actcgcacca cccgcccgcg cctgctgggc 13800 gaggaggagt acctaaacaa ctcgctgctg cagccgcagc gcgaaaaaaa cctgcctccg 13860 gcatttccca acaacgggat agagagccta gtggacaaga tgagtagatg gaagacgtac 13920 gcgcaggagc acagggacgt gccaggcccg cgcccgccca cccgtcgtca aaggcacgac 13980 cgtcagcggg gtctggtgtg ggaggacgat gactcggcag acgacagcag cgtcctggat 14040 ttgggaggga gtggcaaccc gtttgcgcac cttcgcccca ggctggggag aatgttttaa 14100 aaaaaaaaaa gcatgatgca aaataaaaaa ctcaccaagg ccatggcacc gagcgttggt 14160 tttcttgtat tccccttagt atgcggcgcg cggcgatgta tgaggaaggt cctcctccct 14220 cctacgagag tgtggtgagc gcggcgccag tggcggcggc gctgggttct cccttcgatg 14280 ctcccctgga cccgccgttt gtgcctccgc ggtacctgcg gcctaccggg gggagaaaca 14340 gcatccgtta ctctgagttg gcacccctat tcgacaccac ccgtgtgtac ctggtggaca 14400 acaagtcaac ggatgtggca tccctgaact accagaacga ccacagcaac tttctgacca 14460 cggtcattca aaacaatgac tacagcccgg gggaggcaag cacacagacc atcaatcttg 14520 acgaccggtc gcactggggc ggcgacctga aaaccatcct gcataccaac atgccaaatg 14580 tgaacgagtt catgtttacc aataagttta aggcgcgggt gatggtgtcg cgcttgccta 14640 ctaaggacaa tcaggtggag ctgaaatacg agtgggtgga gttcacgctg cccgagggca 14700 actactccga gaccatgacc atagacctta tgaacaacgc gatcgtggag cactacttga 14760 aagtgggcag acagaacggg gttctggaaa gcgacatcgg ggtaaagttt gacacccgca 14820 acttcagact ggggtttgac cccgtcactg gtcttgtcat gcctggggta tatacaaacg 14880 aagccttcca tccagacatc attttgctgc caggatgcgg ggtggacttc acccacagcc 14940 gcctgagcaa cttgttgggc atccgcaagc ggcaaccctt ccaggagggc tttaggatca 15000 cctacgatga tctggagggt ggtaacattc ccgcactgtt ggatgtggac gcctaccagg 15060 cgagcttgaa agatgacacc gaacagggcg ggggtggcgc aggcggcagc aacagcagtg 15120 gcagcggcgc ggaagagaac tccaacgcgg cagccgcggc aatgcagccg gtggaggaca 15180 tgaacgatca tgccattcgc ggcgacacct ttgccacacg ggctgaggag aagcgcgctg 15240 aggccgaagc agcggccgaa gctgccgccc ccgctgcgca acccgaggtc gagaagcctc 15300 agaagaaacc ggtgatcaaa cccctgacag aggacagcaa gaaacgcagt tacaacctaa 15360 taagcaatga cagcaccttc acccagtacc gcagctggta ccttgcatac aactacggcg 15420 accctcagac cggaatccgc tcatggaccc tgctttgcac tcctgacgta acctgcggct 15480 cggagcaggt ctactggtcg ttgccagaca tgatgcaaga ccccgtgacc ttccgctcca 15540 cgcgccagat cagcaacttt ccggtggtgg gcgccgagct gttgcccgtg cactccaaga 15600 gcttctacaa cgaccaggcc gtctactccc aactcatccg ccagtttacc tctctgaccc 15660 acgtgttcaa tcgctttccc gagaaccaga ttttggcgcg cccgccagcc cccaccatca 15720 ccaccgtcag tgaaaacgtt cctgctctca cagatcacgg gacgctaccg ctgcgcaaca 15780 gcatcggagg agtccagcga gtgaccatta ctgacgccag acgccgcacc tgcccctacg 15840 tttacaaggc cctgggcata gtctcgccgc gcgtcctatc gagccgcact ttttgagcaa 15900 gcatgtccat ccttatatcg cccagcaata acacaggctg gggcctgcgc ttcccaagca 15960 agatgtttgg cggggccaag aagcgctccg accaacaccc agtgcgcgtg cgcgggcact 16020 accgcgcgcc ctggggcgcg cacaaacgcg gccgcactgg gcgcaccacc gtcgatgacg 16080 ccatcgacgc ggtggtggag gaggcgcgca actacacgcc cacgccgcca ccagtgtcca 16140 cagtggacgc ggccattcag accgtggtgc gcggagcccg gcgctatgct aaaatgaaga 16200 gacggcggag gcgcgtagca cgtcgccacc gccgccgacc cggcactgcc gcccaacgcg 16260 cggcggcggc cctgcttaac cgcgcacgtc gcaccggccg acgggcggcc atgcgggccg 16320 ctcgaaggct ggccgcgggt attgtcactg tgccccccag gtccaggcga cgagcggccg 16380 ccgcagcagc cgcggccatt agtgctatga ctcagggtcg caggggcaac gtgtattggg 16440 tgcgcgactc ggttagcggc ctgcgcgtgc ccgtgcgcac ccgccccccg cgcaactaga 16500 ttgcaagaaa aaactactta gactcgtact gttgtatgta tccagcggcg gcggcgcgca 16560 acgaagctat gtccaagcgc aaaatcaaag aagagatgct ccaggtcatc gcgccggaga 16620 tctatggccc cccgaagaag gaagagcagg attacaagcc ccgaaagcta aagcgggtca 16680 aaaagaaaaa gaaagatgat gatgatgaac ttgacgacga ggtggaactg ctgcacgcta 16740 ccgcgcccag gcgacgggta cagtggaaag gtcgacgcgt aaaacgtgtt ttgcgacccg 16800 gcaccaccgt agtctttacg cccggtgagc gctccacccg cacctacaag cgcgtgtatg 16860 atgaggtgta cggcgacgag gacctgcttg agcaggccaa cgagcgcctc ggggagtttg 16920 cctacggaaa gcggcataag gacatgctgg cgttgccgct ggacgagggc aacccaacac 16980 ctagcctaaa gcccgtaaca ctgcagcagg tgctgcccgc gcttgcaccg tccgaagaaa 17040 agcgcggcct aaagcgcgag tctggtgact tggcacccac cgtgcagctg atggtaccca 17100 agcgccagcg actggaagat gtcttggaaa aaatgaccgt ggaacctggg ctggagcccg 17160 aggtccgcgt gcggccaatc aagcaggtgg cgccgggact gggcgtgcag accgtggacg 17220 ttcagatacc cactaccagt agcaccagta ttgccaccgc cacagagggc atggagacac 17280 aaacgtcccc ggttgcctca gcggtggcgg atgccgcggt gcaggcggtc gctgcggccg 17340 cgtccaagac ctctacggag gtgcaaacgg acccgtggat gtttcgcgtt tcagcccccc 17400 ggcgcccgcg cggttcgagg aagtacggcg ccgccagcgc gctactgccc gaatatgccc 17460 tacatccttc cattgcgcct acccccggct atcgtggcta cacctaccgc cccagaagac 17520 gagcaactac ccgacgccga accaccactg gaacccgccg ccgccgtcgc cgtcgccagc 17580 ccgtgctggc cccgatttcc gtgcgcaggg tggctcgcga aggaggcagg accctggtgc 17640 tgccaacagc gcgctaccac cccagcatcg tttaaaagcc ggtctttgtg gttcttgcag 17700 atatggccct cacctgccgc ctccgtttcc cggtgccggg attccgagga agaatgcacc 17760 gtaggagggg catggccggc cacggcctga cgggcggcat gcgtcgtgcg caccaccggc 17820 ggcggcgcgc gtcgcaccgt cgcatgcgcg gcggtatcct gcccctcctt attccactga 17880 tcgccgcggc gattggcgcc gtgcccggaa ttgcatccgt ggccttgcag gcgcagagac 17940 actgattaaa aacaagttgc atgtggaaaa atcaaaataa aaagtctgga ctctcacgct 18000 cgcttggtcc tgtaactatt ttgtagaatg gaagacatca actttgcgtc tctggccccg 18060 cgacacggct cgcgcccgtt catgggaaac tggcaagata tcggcaccag caatatgagc 18120 ggtggcgcct tcagctgggg ctcgctgtgg agcggcatta aaaatttcgg ttccaccgtt 18180 aagaactatg gcagcaaggc ctggaacagc agcacaggcc agatgctgag ggataagttg 18240 aaagagcaaa atttccaaca aaaggtggta gatggcctgg cctctggcat tagcggggtg 18300 gtggacctgg ccaaccaggc agtgcaaaat aagattaaca gtaagcttga tccccgccct 18360 cccgtagagg agcctccacc ggccgtggag acagtgtctc cagaggggcg tggcgaaaag 18420 cgtccgcgcc ccgacaggga agaaactctg gtgacgcaaa tagacgagcc tccctcgtac 18480 gaggaggcac taaagcaagg cctgcccacc acccgtccca tcgcgcccat ggctaccgga 18540 gtgctgggcc agcacacacc cgtaacgctg gacctgcctc cccccgccga cacccagcag 18600 aaacctgtgc tgccaggccc gaccgccgtt gttgtaaccc gtcctagccg cgcgtccctg 18660 cgccgcgccg ccagcggtcc gcgatcgttg cggcccgtag ccagtggcaa ctggcaaagc 18720 acactgaaca gcatcgtggg tctgggggtg caatccctga agcgccgacg atgcttctga 18780 atagctaacg tgtcgtatgt gtgtcatgta tgcgtccatg tcgccgccag aggagctgct 18840 gagccgccgc gcgcccgctt tccaagatgg ctaccccttc gatgatgccg cagtggtctt 18900 acatgcacat ctcgggccag gacgcctcgg agtacctgag ccccgggctg gtgcagtttg 18960 cccgcgccac cgagacgtac ttcagcctga ataacaagtt tagaaacccc acggtggcgc 19020 ctacgcacga cgtgaccaca gaccggtccc agcgtttgac gctgcggttc atccctgtgg 19080 accgtgagga tactgcgtac tcgtacaagg cgcggttcac cctagctgtg ggtgataacc 19140 gtgtgctgga catggcttcc acgtactttg acatccgcgg cgtgctggac aggggcccta 19200 cttttaagcc ctactctggc actgcctaca acgccctggc tcccaagggt gccccaaatc 19260 cttgcgaatg ggatgaagct gctactgctc ttgaaataaa cctagaagaa gaggacgatg 19320 acaacgaaga cgaagtagac gagcaagctg agcagcaaaa aactcacgta tttgggcagg 19380 cgccttattc tggtataaat attacaaagg agggtattca aataggtgtc gaaggtcaaa 19440 cacctaaata tgccgataaa acatttcaac ctgaacctca aataggagaa tctcagtggt 19500 acgaaactga aattaatcat gcagctggga gagtccttaa aaagactacc ccaatgaaac 19560 catgttacgg ttcatatgca aaacccacaa atgaaaatgg agggcaaggc attcttgtaa 19620 agcaacaaaa tggaaagcta gaaagtcaag tggaaatgca atttttctca actactgagg 19680 cgaccgcagg caatggtgat aacttgactc ctaaagtggt attgtacagt gaagatgtag 19740 atatagaaac cccagacact catatttctt acatgcccac tattaaggaa ggtaactcac 19800 gagaactaat gggccaacaa tctatgccca acaggcctaa ttacattgct tttagggaca 19860 attttattgg tctaatgtat tacaacagca cgggtaatat gggtgttctg gcgggccaag 19920 catcgcagtt gaatgctgtt gtagatttgc aagacagaaa cacagagctt tcataccagc 19980 ttttgcttga ttccattggt gatagaacca ggtacttttc tatgtggaat caggctgttg 20040 acagctatga tccagatgtt agaattattg aaaatcatgg aactgaagat gaacttccaa 20100 attactgctt tccactggga ggtgtgatta atacagagac tcttaccaag gtaaaaccta 20160 aaacaggtca ggaaaatgga tgggaaaaag atgctacaga attttcagat aaaaatgaaa 20220 taagagttgg aaataatttt gccatggaaa tcaatctaaa tgccaacctg tggagaaatt 20280 tcctgtactc caacatagcg ctgtatttgc ccgacaagct aaagtacagt ccttccaacg 20340 taaaaatttc tgataaccca aacacctacg actacatgaa caagcgagtg gtggctcccg 20400 ggttagtgga ctgctacatt aaccttggag cacgctggtc ccttgactat atggacaacg 20460 tcaacccatt taaccaccac cgcaatgctg gcctgcgcta ccgctcaatg ttgctgggca 20520 atggtcgcta tgtgcccttc cacatccagg tgcctcagaa gttctttgcc attaaaaacc 20580 tccttctcct gccgggctca tacacctacg agtggaactt caggaaggat gttaacatgg 20640 ttctgcagag ctccctagga aatgacctaa gggttgacgg agccagcatt aagtttgata 20700 gcatttgcct ttacgccacc ttcttcccca tggcccacaa caccgcctcc acgcttgagg 20760 ccatgcttag aaacgacacc aacgaccagt cctttaacga ctatctctcc gccgccaaca 20820 tgctctaccc tatacccgcc aacgctacca acgtgcccat atccatcccc tcccgcaact 20880 gggcggcttt ccgcggctgg gccttcacgc gccttaagac taaggaaacc ccatcactgg 20940 gctcgggcta cgacccttat tacacctact ctggctctat accctaccta gatggaacct 21000 tttacctcaa ccacaccttt aagaaggtgg ccattacctt tgactcttct gtcagctggc 21060 ctggcaatga ccgcctgctt acccccaacg agtttgaaat taagcgctca gttgacgggg 21120 agggttacaa cgttgcccag tgtaacatga ccaaagactg gttcctggta caaatgctag 21180 ctaactacaa cattggctac cagggcttct atatcccaga gagctacaag gaccgcatgt 21240 actccttctt tagaaacttc cagcccatga gccgtcaggt ggtggatgat actaaataca 21300 aggactacca acaggtgggc atcctacacc aacacaacaa ctctggattt gttggctacc 21360 ttgcccccac catgcgcgaa ggacaggcct accctgctaa cttcccctat ccgcttatag 21420 gcaagaccgc agttgacagc attacccaga aaaagtttct ttgcgatcgc accctttggc 21480 gcatcccatt ctccagtaac tttatgtcca tgggcgcact cacagacctg ggccaaaacc 21540 ttctctacgc caactccgcc cacgcgctag acatgacttt tgaggtggat cccatggacg 21600 agcccaccct tctttatgtt ttgtttgaag tctttgacgt ggtccgtgtg caccggccgc 21660 accgcggcgt catcgaaacc gtgtacctgc gcacgccctt ctcggccggc aacgccacaa 21720 cataaagaag caagcaacat caacaacagc tgccgccatg ggctccagtg agcaggaact 21780 gaaagccatt gtcaaagatc ttggttgtgg gccatatttt ttgggcacct atgacaagcg 21840 ctttccaggc tttgtttctc cacacaagct cgcctgcgcc atagtcaata cggccggtcg 21900 cgagactggg ggcgtacact ggatggcctt tgcctggaac ccgcactcaa aaacatgcta 21960 cctctttgag ccctttggct tttctgacca gcgactcaag caggtttacc agtttgagta 22020 cgagtcactc ctgcgccgta gcgccattgc ttcttccccc gaccgctgta taacgctgga 22080 aaagtccacc caaagcgtac aggggcccaa ctcggccgcc tgtggactat tctgctgcat 22140 gtttctccac gcctttgcca actggcccca aactcccatg gatcacaacc ccaccatgaa 22200 ccttattacc ggggtaccca actccatgct caacagtccc caggtacagc ccaccctgcg 22260 tcgcaaccag gaacagctct acagcttcct ggagcgccac tcgccctact tccgcagcca 22320 cagtgcgcag attaggagcg ccacttcttt ttgtcacttg aaaaacatgt aaaaataatg 22380 tactagagac actttcaata aaggcaaatg cttttatttg tacactctcg ggtgattatt 22440 tacccccacc cttgccgtct gcgccgttta aaaatcaaag gggttctgcc gcgcatcgct 22500 atgcgccact ggcagggaca cgttgcgata ctggtgttta gtgctccact taaactcagg 22560 cacaaccatc cgcggcagct cggtgaagtt ttcactccac aggctgcgca ccatcaccaa 22620 cgcgtttagc aggtcgggcg ccgatatctt gaagtcgcag ttggggcctc cgccctgcgc 22680 gcgcgagttg cgatacacag ggttgcagca ctggaacact atcagcgccg ggtggtgcac 22740 gctggccagc acgctcttgt cggagatcag atccgcgtcc aggtcctccg cgttgctcag 22800 ggcgaacgga gtcaactttg gtagctgcct tcccaaaaag ggcgcgtgcc caggctttga 22860 gttgcactcg caccgtagtg gcatcaaaag gtgaccgtgc ccggtctggg cgttaggata 22920 cagcgcctgc ataaaagcct tgatctgctt aaaagccacc tgagcctttg cgccttcaga 22980 gaagaacatg ccgcaagact tgccggaaaa ctgattggcc ggacaggccg cgtcgtgcac 23040 gcagcacctt gcgtcggtgt tggagatctg caccacattt cggccccacc ggttcttcac 23100 gatcttggcc ttgctagact gctccttcag cgcgcgctgc ccgttttcgc tcgtcacatc 23160 catttcaatc acgtgctcct tatttatcat aatgcttccg tgtagacact taagctcgcc 23220 ttcgatctca gcgcagcggt gcagccacaa cgcgcagccc gtgggctcgt gatgcttgta 23280 ggtcacctct gcaaacgact gcaggtacgc ctgcaggaat cgccccatca tcgtcacaaa 23340 ggtcttgttg ctggtgaagg tcagctgcaa cccgcggtgc tcctcgttca gccaggtctt 23400 gcatacggcc gccagagctt ccacttggtc aggcagtagt ttgaagttcg cctttagatc 23460 gttatccacg tggtacttgt ccatcagcgc gcgcgcagcc tccatgccct tctcccacgc 23520 agacacgatc ggcacactca gcgggttcat caccgtaatt tcactttccg cttcgctggg 23580 ctcttcctct tcctcttgcg tccgcatacc acgcgccact gggtcgtctt cattcagccg 23640 ccgcactgtg cgcttacctc ctttgccatg cttgattagc accggtgggt tgctgaaacc 23700 caccatttgt agcgccacat cttctctttc ttcctcgctg tccacgatta cctctggtga 23760 tggcgggcgc tcgggcttgg gagaagggcg cttctttttc ttcttgggcg caatggccaa 23820 atccgccgcc gaggtcgatg gccgcgggct gggtgtgcgc ggcaccagcg cgtcttgtga 23880 tgagtcttcc tcgtcctcgg actcgatacg ccgcctcatc cgcttttttg ggggcgcccg 23940 gggaggcggc ggcgacgggg acggggacga cacgtcctcc atggttgggg gacgtcgcgc 24000 cgcaccgcgt ccgcgctcgg gggtggtttc gcgctgctcc tcttcccgac tggccatttc 24060 cttctcctat aggcagaaaa agatcatgga gtcagtcgag aagaaggaca gcctaaccgc 24120 cccctctgag ttcgccacca ccgcctccac cgatgccgcc aacgcgccta ccaccttccc 24180 cgtcgaggca cccccgcttg aggaggagga agtgattatc gagcaggacc caggttttgt 24240 aagcgaagac gacgaggacc gctcagtacc aacagaggat aaaaagcaag accaggacaa 24300 cgcagaggca aacgaggaac aagtcgggcg gggggacgaa aggcatggcg actacctaga 24360 tgtgggagac gacgtgctgt tgaagcatct gcagcgccag tgcgccatta tctgcgacgc 24420 gttgcaagag cgcagcgatg tgcccctcgc catagcggat gtcagccttg cctacgaacg 24480 ccacctattc tcaccgcgcg taccccccaa acgccaagaa aacggcacat gcgagcccaa 24540 cccgcgcctc aacttctacc ccgtatttgc cgtgccagag gtgcttgcca cctatcacat 24600 ctttttccaa aactgcaaga tacccctatc ctgccgtgcc aaccgcagcc gagcggacaa 24660 gcagctggcc ttgcggcagg gcgctgtcat acctgatatc gcctcgctca acgaagtgcc 24720 aaaaatcttt gagggtcttg gacgcgacga gaagcgcgcg gcaaacgctc tgcaacagga 24780 aaacagcgaa aatgaaagtc actctggagt gttggtggaa ctcgagggtg acaacgcgcg 24840 cctagccgta ctaaaacgca gcatcgaggt cacccacttt gcctacccgg cacttaacct 24900 accccccaag gtcatgagca cagtcatgag tgagctgatc gtgcgccgtg cgcagcccct 24960 ggagagggat gcaaatttgc aagaacaaac agaggagggc ctacccgcag ttggcgacga 25020 gcagctagcg cgctggcttc aaacgcgcga gcctgccgac ttggaggagc gacgcaaact 25080 aatgatggcc gcagtgctcg ttaccgtgga gcttgagtgc atgcagcggt tctttgctga 25140 cccggagatg cagcgcaagc tagaggaaac attgcactac acctttcgac agggctacgt 25200 acgccaggcc tgcaagatct ccaacgtgga gctctgcaac ctggtctcct accttggaat 25260 tttgcacgaa aaccgccttg ggcaaaacgt gcttcattcc acgctcaagg gcgaggcgcg 25320 ccgcgactac gtccgcgact gcgtttactt atttctatgc tacacctggc agacggccat 25380 gggcgtttgg cagcagtgct tggaggagtg caacctcaag gagctgcaga aactgctaaa 25440 gcaaaacttg aaggacctat ggacggcctt caacgagcgc tccgtggccg cgcacctggc 25500 ggacatcatt ttccccgaac gcctgcttaa aaccctgcaa cagggtctgc cagacttcac 25560 cagtcaaagc atgttgcaga actttaggaa ctttatccta gagcgctcag gaatcttgcc 25620 cgccacctgc tgtgcacttc ctagcgactt tgtgcccatt aagtaccgcg aatgccctcc 25680 gccgctttgg ggccactgct accttctgca gctagccaac taccttgcct accactctga 25740 cataatggaa gacgtgagcg gtgacggtct actggagtgt cactgtcgct gcaacctatg 25800 caccccgcac cgctccctgg tttgcaattc gcagctgctt aacgaaagtc aaattatcgg 25860 tacctttgag ctgcagggtc cctcgcctga cgaaaagtcc gcggctccgg ggttgaaact 25920 cactccgggg ctgtggacgt cggcttacct tcgcaaattt gtacctgagg actaccacgc 25980 ccacgagatt aggttctacg aagaccaatc ccgcccgcca aatgcggagc ttaccgcctg 26040 cgtcattacc cagggccaca ttcttggcca attgcaagcc atcaacaaag cccgccaaga 26100 gtttctgcta cgaaagggac ggggggttta cttggacccc cagtccggcg aggagctcaa 26160 cccaatcccc ccgccgccgc agccctatca gcagcagccg cgggcccttg cttcccagga 26220 tggcacccaa aaagaagctg cagctgccgc cgccacccac ggacgaggag gaatactggg 26280 acagtcaggc agaggaggtt ttggacgagg aggaggagga catgatggaa gactgggaga 26340 gcctagacga ggaagcttcc gaggtcgaag aggtgtcaga cgaaacaccg tcaccctcgg 26400 tcgcattccc ctcgccggcg ccccagaaat cggcaaccgg ttccagcatg gctacaacct 26460 ccgctcctca ggcgccgccg gcactgcccg ttcgccgacc caaccgtaga tgggacacca 26520 ctggaaccag ggccggtaag tccaagcagc cgccgccgtt agcccaagag caacaacagc 26580 gccaaggcta ccgctcatgg cgcgggcaca agaacgccat agttgcttgc ttgcaagact 26640 gtgggggcaa catctccttc gcccgccgct ttcttctcta ccatcacggc gtggccttcc 26700 cccgtaacat cctgcattac taccgtcatc tctacagccc atactgcacc ggcggcagcg 26760 gcagcggcag caacagcagc ggccacacag aagcaaaggc gaccggatag caagactctg 26820 acaaagccca agaaatccac agcggcggca gcagcaggag gaggagcgct gcgtctggcg 26880 cccaacgaac ccgtatcgac ccgcgagctt agaaacagga tttttcccac tctgtatgct 26940 atatttcaac agagcagggg ccaagaacaa gagctgaaaa taaaaaacag gtctctgcga 27000 tccctcaccc gcagctgcct gtatcacaaa agcgaagatc agcttcggcg cacgctggaa 27060 gacgcggagg ctctcttcag taaatactgc gcgctgactc ttaaggacta gtttcgcgcc 27120 ctttctcaaa tttaagcgcg aaaactacgt catctccagc ggccacaccc ggcgccagca 27180 cctgtcgtca gcgccatttc aactttgtat acaaaagttg tgatgagcaa ggaaattccc 27240 acgccctaca tgtggagtta ccagccacaa atgggacttg cggctggagc tgcccaagac 27300 tactcaaccc gaataaacta catgagcgcg ggaccccaca tgatatcccg ggtcaacgga 27360 atacgcgccc accgaaaccg aattctcctg gaacaggcgg ctattaccac cacacctcgt 27420 aataacctta atccccgtag ttggcccgct gccctggtgt accaggaaag tcccgctccc 27480 accactgtgg tacttcccag agacgcccag gccgaagttc agatgactaa ctcaggggcg 27540 cagcttgcgg gcggctttcg tcacagggtg cggtcgcccg ggcagggtat aactcacctg 27600 acaatcagag ggcgaggtat tcagctcaac gacgagtcgg tgagctcctc gcttggtctc 27660 cgtccggacg ggacatttca gatcggcggc gccggccgct cttcattcac gcctcgtcag 27720 gcaatcctaa ctctgcagac ctcgtcctct gagccgcgct ctggaggcat tggaactctg 27780 caatttattg aggagtttgt gccatcggtc tactttaacc ccttctcggg acctcccggc 27840 cactatccgg atcaatttat tcctaacttt gacgcggtaa aggactcggc ggacggctac 27900 gactgaatgt taagtggaga ggcagagcaa ctgcgcctga aacacctggt ccactgtcgc 27960 cgccacaagt gctttgcccg cgactccggt gagttttgct actttgaatt gcccgaggat 28020 catatcgagg gcccggcgca cggcgtccgg cttaccgccc agggagagct tgcccgtagc 28080 ctgattcggg agtttaccca gcgccccctg ctagttgagc gggacagggg accctgtgtt 28140 ctcactgtga tttgcaactg tcctaaccct ggattacatc aagatctttg ttgccatctc 28200 tgtgctgagt ataataaata cagaaattaa aatatactgg ggctcctatc gccatcctgt 28260 aaacgccacc gtcttcaccc gcccaagcaa accaaggcga accttacctg gtacttttaa 28320 catctctccc tctgtgattt acaacagttt caacccagac ggagtgagtc tacgagagaa 28380 cctctccgag ctcagctact ccatcagaaa aaacaccacc ctccttacct gccgggaacg 28440 tacgagtgcg tcaccggccg ctgcaccaca cctaccgcct gaccgtaaac cagacttttt 28500 ccggacagac ctcaataact ctgtttacca gaacaggagg tgagcttaga aaacccttag 28560 ggtattaggc caaaggcgca gctactgtgg ggtttatgaa caattcaagc aactctacgg 28620 gctattctaa ttcaggtttc tctagaatcg gggttggggt tattctctgt cttgtgattc 28680 tctttattct tatactaacg cttctctgcc taaggctcgc cgcctgctgt gtgcacattt 28740 gcatttattg tcagcttttt aaacgctggg gtcgccaccc aagatgatta ggtacataat 28800 cctaggttta ctcacccttg cgtcagccca cggtaccacc caaaaggtgg attttaagga 28860 gccagcctgt aatgttacat tcgcagctga agctaatgag tgcaccactc ttataaaatg 28920 caccacagaa catgaaaagc tgcttattcg ccacaaaaac aaaattggca agtatgctgt 28980 ttatgctatt tggcagccag gtgacactac agagtataat gttacagttt tccagggtaa 29040 aagtcataaa acttttatgt atacttttcc attttatgaa atgtgcgaca ttaccatgta 29100 catgagcaaa cagtataagt tgtggccccc acaaaattgt gtggaaaaca ctggcacttt 29160 ctgctgcact gctatgctaa ttacagtgct cgctttggtc tgtaccctac tctatattaa 29220 atacaaaagc agacgcagct ttattgagga aaagaaaatg ccttaattta ctaagttaca 29280 aagctaatgt caccactaac tgctttactc gctgcttgca aaacaaattc aaaaagttag 29340 cattataatt agaataggat ttaaaccccc cggtcatttc ctgctcaata ccattcccct 29400 gaacaattga ctctatgtgg gatatgctcc agcgctacaa ccttgaagtc aggcttcctg 29460 gatgtcagca tctgactttg gccagcacct gtcccgcgga tttgttccag tccaactaca 29520 gcgacccacc ctaacagaga tgaccaacac aaccaacgcg gccgccgcta ccggacttac 29580 atctaccaca aatacacccc aagtttctgc ctttgtcaat aactgggata acttgggcat 29640 gtggtggttc tccatagcgc ttatgtttgt atgccttatt attatgtggc tcatctgctg 29700 cctaaagcgc aaacgcgccc gaccacccat ctatagtccc atcattgtgc tacacccaaa 29760 caatgatgga atccatagat tggacggact gaaacacatg ttcttttctc ttacagtatg 29820 attaaatgag acatgattcc tcgagttttt atattactga cccttgttgc gcttttttgt 29880 gcgtgctcca cattggctgc ggtttctcac atcgaagtag actgcattcc agccttcaca 29940 gtctatttgc tttacggatt tgtcaccctc acgctcatct gcagcctcat cactgtggtc 30000 atcgccttta tccagtgcat tgactgggtc tgtgtgcgct ttgcatatct cagacaccat 30060 ccccagtaca gggacaggac tatagctgag cttcttagaa ttctttaatt atgaaattta 30120 ctgtgacttt tctgctgatt atttgcaccc tatctgcgtt ttgttccccg acctccaagc 30180 ctcaaagaca tatatcatgc agattcactc gtatatggaa tattccaagt tgctacaatg 30240 aaaaaagcga tctttccgaa gcctggttat atgcaatcat ctctgttatg gtgttctgca 30300 gtaccatctt agccctagct atatatccct accttgacat tggctggaac gcaatagatg 30360 ccatgaacca cccaactttc cccgcgcccg ctatgcttcc actgcaacaa gttgttgccg 30420 gcggctttgt cccagccaat cagcctcgcc caccttctcc cacccccact gaaatcagct 30480 actttaatct aacaggagga gatgactgac accctagatc tagaaatgga cggaattatt 30540 acagagcagc gcctgctaga aagacgcagg gcagcggccg agcaacagcg catgaatcaa 30600 gagctccaag acatggttaa cttgcaccag tgcaaaaggg gtatcttttg tctggtaaag 30660 caggccaaag tcacctacga cagtaatacc accggacacc gccttagcta caagttgcca 30720 accaagcgtc agaaattggt ggtcatggtg ggagaaaagc ccattaccat aactcagcac 30780 tcggtagaaa ccgaaggctg cattcactca ccttgtcaag gacctgagga tctctgcacc 30840 cttattaaga ccctgtgcgg tctcaaagat cttattccct ttaactaata aaaaaaaata 30900 ataaagcatc acttacttaa aatcagttag caaatttctg tccagtttat tcagcagcac 30960 ctccttgccc tcctcccagc tctggtattg cagcttcctc ctggctgcaa actttctcca 31020 caatctaaat ggaatgtcag tttcctcctg ttcctgtcca tccgcaccca ctatcttcat 31080 gttgttgcag atgaagcgcg caagaccgtc tgaagatacc ttcaaccccg tgtatccata 31140 tgacacggaa accggtcctc caactgtgcc ttttcttact cctccctttg tatcccccaa 31200 tgggtttcaa gagagtcccc ctggggtact ctctttgcgc ctatccgaac ctctagttac 31260 ctccaatggc atgcttgcgc tcaaaatggg caacggcctc tctctggacg aggccggcaa 31320 ccttacctcc caaaatgtaa ccactgtgag cccacctctc aaaaaaacca agtcaaacat 31380 aaacctggaa atatctgcac ccctcacagt tacctcagaa gccctaactg tggctgccgc 31440 cgcacctcta atggtcgcgg gcaacacact caccatgcaa tcacaggccc cgctaaccgt 31500 gcacgactcc aaacttagca ttgccaccca aggacccctc acagtgtcag aaggaaagct 31560 agccctgcaa acatcaggcc ccctcaccac caccgatagc agtaccctta ctatcactgc 31620 ctcaccccct ctaactactg ccactggtag cttgggcatt gacttgaaag agcccattta 31680 tacacaaaat ggaaaactag gactaaagta cggggctcct ttgcatgtaa cagacgacct 31740 aaacactttg accgtagcaa ctggtccagg tgtgactatt aataatactt ccttgcaaac 31800 taaagttact ggagccttgg gttttgattc acaaggcaat atgcaactta atgtagcagg 31860 aggactaagg attgattctc aaaacagacg ccttatactt gatgttagtt atccgtttga 31920 tgctcaaaac caactaaatc taagactagg acagggccct ctttttataa actcagccca 31980 caacttggat attaactaca acaaaggcct ttacttgttt acagcttcaa acaattccaa 32040 aaagcttgag gttaacctaa gcactgccaa ggggttgatg tttgacgcta cagccatagc 32100 cattaatgca ggagatgggc ttgaatttgg ttcacctaat gcaccaaaca caaatcccct 32160 caaaacaaaa attggccatg gcctagaatt tgattcaaac aaggctatgg ttcctaaact 32220 aggaactggc cttagttttg acagcacagg tgccattaca gtaggaaaca aaaataatga 32280 taagctaact ttgtggacca caccagctcc atctcctaac tgtagactaa atgcagagaa 32340 agatgctaaa ctcactttgg tcttaacaaa atgtggcagt caaatacttg ctacagtttc 32400 agttttggct gttaaaggca gtttggctcc aatatctgga acagttcaaa gtgctcatct 32460 tattataaga tttgacgaaa atggagtgct actaaacaat tccttcctgg acccagaata 32520 ttggaacttt agaaatggag atcttactga aggcacagcc tatacaaacg ctgttggatt 32580 tatgcctaac ctatcagctt atccaaaatc tcacggtaaa actgccaaaa gtaacattgt 32640 cagtcaagtt tacttaaacg gagacaaaac taaacctgta acactaacca ttacactaaa 32700 cggtacacag gaaacaggag acacaactcc aagtgcatac tctatgtcat tttcatggga 32760 ctggtctggc cacaactaca ttaatgaaat atttgccaca tcctcttaca ctttttcata 32820 cattgcccaa gaaggaagcg gagctactaa cttcagcctg ctgaagcagg ctggtgacgt 32880 cgaggagaat cctggcccaa tggtgagcaa aggcgaagag ctgttcaccg gcgtggtgcc 32940 catcctggtg gagctggacg gcgacgtgaa cggccacaag ttcagcgtga gcggcgaggg 33000 cgagggcgac gccacctacg gcaagctgac cctgaagctg ctgtgcacca ccggcaagct 33060 gcccgtgccc tggcccaccc tggtgaccac cctgggctac ggcgtgcagt gcttcgcccg 33120 gtaccccgac cacatgaagc agcacgactt cttcaagagc gccatgcccg agggctacgt 33180 gcaggagcgg accatcttct tcaaggacga cggcaactac aagacccggg ccgaggtgaa 33240 gttcgagggc gacaccctgg tgaaccggat cgagctgaag ggcatcgact tcaaggagga 33300 cggcaacatc ctgggccaca agctggagta caactacaac agccacaacg tgtacatcac 33360 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccggcaca acatcgagga 33420 cggcggcgtg cagctggccg accactacca gcagaacacc cccatcggcg acggccccgt 33480 gctgctgccc gacaaccact acctgagcta ccagagcgcc ctgttcaagg accccaacga 33540 gaagcgggac cacatggtgc tgctggagtt cctgaccgcc gccggcatca ccgagggcat 33600 gaacgagctc tataagtaat aaagaatcgt ttgtgttatg tttcaacgtg tttatttttc 33660 aattgcagaa aatttcgaat catttttcat tcagtagtat agccccacca ccacatagct 33720 tatacagatc accgtacctc aactttgtat aataaagttg taatcaaact cacagaaccc 33780 tagtattcaa cctgccacct ccctcccaac acacagagta cacagtcctt tctccccggc 33840 tggccttaaa aagcatcata tcatgggtaa cagacatatt cttaggtgtt atattccaca 33900 cggtttcctg tcgagccaaa cgctcatcag tgatattaat aaactccccg ggcagctcac 33960 ttaagttcat gtcgctgtcc agctgctgag ccacaggctg ctgtccaact tgcggttgct 34020 taacgggcgg cgaaggagaa gtccacgcct acatgggggt agagtcataa tcgtgcatca 34080 ggatagggcg gtggtgctgc agcagcgcgc gaataaactg ctgccgccgc cgctccgtcc 34140 tgcaggaata caacatggca gtggtctcct cagcgatgat tcgcaccgcc cgcagcataa 34200 ggcgccttgt cctccgggca cagcagcgca ccctgatctc acttaaatca gcacagtaac 34260 tgcagcacag caccacaata ttgttcaaaa tcccacagtg caaggcgctg tatccaaagc 34320 tcatggcggg gaccacagaa cccacgtggc catcatacca caagcgcagg tagattaagt 34380 ggcgacccct cataaacacg ctggacataa acattacctc ttttggcatg ttgtaattca 34440 ccacctcccg gtaccatata aacctctgat taaacatggc gccatccacc accatcctaa 34500 accagctggc caaaacctgc ccgccggcta tacactgcag ggaaccggga ctggaacaat 34560 gacagtggag agcccaggac tcgtaaccat ggatcatcat gctcgtcatg atatcaatgt 34620 tggcacaaca caggcacacg tgcatacact tcctcaggat tacaagctcc tcccgcgtta 34680 gaaccatatc ccagggaaca acccattcct gaatcagcgt aaatcccaca ctgcagggaa 34740 gacctcgcac gtaactcacg ttgtgcattg tcaaagtgtt acattcgggc agcagcggat 34800 gatcctccag tatggtagcg cgggtttctg tctcaaaagg aggtagacga tccctactgt 34860 acggagtgcg ccgagacaac cgagatcgtg ttggtcgtag tgtcatgcca aatggaacgc 34920 cggacgtagt catatttcct gaagcaaaac caggtgcggg cgtgacaaac agatctgcgt 34980 ctccggtctc gccgcttaga tcgctctgtg tagtagttgt agtatatcca ctctctcaaa 35040 gcatccaggc gccccctggc ttcgggttct atgtaaactc cttcatgcgc cgctgccctg 35100 ataacatcca ccaccgcaga ataagccaca cccagccaac ctacacattc gttctgcgag 35160 tcacacacgg gaggagcggg aagagctgga agaaccatgt tttttttttt attccaaaag 35220 attatccaaa acctcaaaat gaagatctat taagtgaacg cgctcccctc cggtggcgtg 35280 gtcaaactct acagccaaag aacagataat ggcatttgta agatgttgca caatggcttc 35340 caaaaggcaa acggccctca cgtccaagtg gacgtaaagg ctaaaccctt cagggtgaat 35400 ctcctctata aacattccag caccttcaac catgcccaaa taattctcat ctcgccacct 35460 tctcaatata tctctaagca aatcccgaat attaagtccg gccattgtaa aaatctgctc 35520 cagagcgccc tccaccttca gcctcaagca gcgaatcatg attgcaaaaa ttcaggttcc 35580 tcacagacct gtataagatt caaaagcgga acattaacaa aaataccgcg atcccgtagg 35640 tcccttcgca gggccagctg aacataatcg tgcaggtctg cacggaccag cgcggccact 35700 tccccgccag gaaccatgac aaaagaaccc acactgatta tgacacgcat actcggagct 35760 atgctaacca gcgtagcccc gatgtaagct tgttgcatgg gcggcgatat aaaatgcaag 35820 gtgctgctca aaaaatcagg caaagcctcg cgcaaaaaag aaagcacatc gtagtcatgc 35880 tcatgcagat aaaggcaggt aagctccgga accaccacag aaaaagacac catttttctc 35940 tcaaacatgt ctgcgggttt ctgcataaac acaaaataaa ataacaaaaa aacatttaaa 36000 cattagaagc ctgtcttaca acaggaaaaa caacccttat aagcataaga cggactacgg 36060 ccatgccggc gtgaccgtaa aaaaactggt caccgtgatt aaaaagcacc accgacagct 36120 cctcggtcat gtccggagtc ataatgtaag actcggtaaa cacatcaggt tgattcacat 36180 cggtcagtgc taaaaagcga ccgaaatagc ccgggggaat acatacccgc aggcgtagag 36240 acaacattac agcccccata ggaggtataa caaaattaat aggagagaaa aacacataaa 36300 cacctgaaaa accctcctgc ctaggcaaaa tagcaccctc ccgctccaga acaacataca 36360 gcgcttccac agcggcagcc ataacagtca gccttaccag taaaaaagaa aacctattaa 36420 aaaaacacca ctcgacacgg caccagctca atcagtcaca gtgtaaaaaa gggccaagtg 36480 cagagcgagt atatatagga ctaaaaaatg acgtaacggt taaagtccac aaaaaacacc 36540 cagaaaaccg cacgcgaacc tacgcccaga aacgaaagcc aaaaaaccca caacttcctc 36600 aaatcgtcac ttccgttttc ccacgttacg tcacttccca ttttaagaaa actacaattc 36660 ccaacacata caagttactc cgccctaaaa cctacgtcac ccgccccgtt cccacgcccc 36720 gcgccacgtc acaaactcca ccccctcatt atcatattgg cttcaatcca aaataaggta 36780 tattattgat gatg 36794 <210> 14 <211> 19 <212> PRT <213> Artificial Sequence <220> <223> Synthetic peptide <400> 14 Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 1 5 10 15 Pro Gly Pro <210> 15 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> Synthetic peptide <400> 15 Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 1 5 10 15 Glu Ser Asn Pro Gly Pro 20 <210> 16 <211> 20 <212> PRT <213> Artificial Sequence <220> <223> Synthetic peptide <400> 16 Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 1 5 10 15 Asn Pro Gly Pro 20 <210> 17 <211> 18 <212> PRT <213> Artificial Sequence <220> <223> Synthetic peptide <400> 17 Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 1 5 10 15 Gly Pro <210> 18 <211> 22 <212> PRT <213> Artificial Sequence <220> <223> Synthetic peptide <400> 18 Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 1 5 10 15 Glu Glu Asn Pro Gly Pro 20 <210> 19 <211> 25 <212> PRT <213> Artificial Sequence <220> <223> Synthetic peptide <400> 19 Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 1 5 10 15 Gly Asp Val Glu Ser Asn Pro Gly Pro 20 25 <210> 20 <211> 23 <212> PRT <213> Artificial Sequence <220> <223> Synthetic peptide <400> 20 Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 1 5 10 15 Val Glu Ser Asn Pro Gly Pro 20 <210> 21 <211> 21 <212> PRT <213> Artificial Sequence <220> <223> Synthetic peptide <400> 21 Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 1 5 10 15 Glu Asn Pro Gly Pro 20 <210> 22 <211> 35876 <212> DNA <213> Artificial Sequence <220> <223> Synthetic adenovirus genome Ad9 E3-15k-P2A-YPet <400> 22 ctatctatat aatatacccc acaaagtaaa caaaagttaa tatgcaaatg agcttttgaa 60 ttttaacggt ttcggggcgg agccaacgct gattggacga gagaagacga tgcaaatgac 120 gtcacgacgc acggctaacg gtcgccgcgg aggcgtggcc tagcccggaa gcaagtcgcg 180 gggctgatga cgtataaaaa agcggacttt agacccggaa acggccgatt ttcccgcggc 240 cacgcccgga tatgaggtaa ttctgggcgg atgcaagtga aattaggtca ttttggcgcg 300 aaaactgaat gaggaagtga aaagcgaaaa ataccggtcc ctcccagggc ggaatattta 360 ccgagggccg agagactttg accgattacg tgggggtttc gattgcggtg tttttttcgc 420 gaatttccgc gtccgtgtca aagtccggtg tttatgtcac agatcagctg atccgcaggg 480 tatttaaacc agtcgagtcc gtcaagaggc cactcttgag tgccagcgag tagagatttc 540 tctgagctcc gctcccagag accgagaaaa atgagacacc tgcgcctcct gccttcaact 600 gtgcccggtg agctggctgt gcttatgctg gaggactttg tggatacagt attggaggac 660 gaactgcatc caagtccgtt cgagctggga cccacacttc aggatctcta tgatctggag 720 gtagatgccc atgatgacga ccctaacgag gaggctgtga atttaatatt tccagaatct 780 atgattcttc aggctgacat agccaacgaa tctactccac ttcatacacc gactctgtca 840 cccatacctg aattggaaga ggaggacgaa ctagacctcc ggtgttatga ggaaggtttt 900 cctcccagcg attcagagga tgaacggggt gagcagacca tggctctgat ctcagactat 960 gcttgtgtga ttgtggagga gcaagatgtg attgaaaaat ctactgagcc agtacaaggc 1020 tgtaggaact gccagtacca ccgggataag tccggagatg tgaacgcctc ctgcgctttg 1080 tgctatatga aacagacttt cagctttatt tacagtaagt ggagtgaatg tgagagaggc 1140 tgagtgctta acacataact gtaatgcttg aacagctgtg ctaagtgtgg tttattttgt 1200 tactaggtcc ggtgtcagag gatgagttat caccctcaga agaagaccac ccgtctcccc 1260 ctgagctgtc aggcgaaacg cccctgcaag tgttcagacc caccccagtc agacccagtg 1320 gcgagaggcg agcggctgtt gacaaaattg aggacttgtt gcaggacatg ggtggggatg 1380 aacctttgga cctgagcttg aaacgcccca ggaactagac gcagctgcgc ttagtcatgt 1440 gtaaataaag ttgtacaata aaagtctatg tgacgcatgc aaggtgtggt ttatgactca 1500 tgggcggggc ttagtcctat ataagtggca acacctgggc acttgggcac agaccttcag 1560 ggagttcctg atggatgtgt ggactatcct tgcagacttt agcaagacac gccggcttgt 1620 agaggatagt tcagacgggt gctccgggtt ctggagacac tggtttggaa ctcctctatc 1680 tcgcctggtg tacacagtta agaaggatta taaagaggaa tttgaaaata tttttgctga 1740 ctgctctggc ctgctagatt ctctgaatct tggccaccag tcccttttcc aggaaagggt 1800 actccacagc cttgattttt ccagcccagg gcgcactaca gccggggttg cttttgtggt 1860 ttttctggtt gacaaatgga gccaggacac ccaactgagc aggggctaca tcctggactt 1920 cgcagccatg cacctgtgga gggcctggat caggcagcgg ggacagagaa tcttgaatta 1980 ctggcttcta cagccagcag ctccgggtct tcttcgtcta cacagacaaa catccatgtt 2040 ggaggaagaa atgaggcagg ccatggacga gaacccgagg agcggcctgg accctccgtc 2100 ggaagaggag ctggattgaa tcaggtatcc agcctgtacc cagagcttag caaggtgctg 2160 acatccatgg ccaggggagt taagagggag aggagcgatg ggggtaatac cgggatgatg 2220 accgagctga cggccagcct gatgaatcgg aagcgcccag agcgccttac ctggtacgag 2280 ctacagcagg agtgcaggga tgagttgggc ctgatgcagg ataaatatgg cctggagcag 2340 ataaaaaccc attggttgaa cccagatgag gattgggagg aggctattaa gaagtatgcc 2400 aagatagccc tgcgcccaga ttgcaagtac atagtgacca agaccgtgaa tatcagacat 2460 gcctgctaca tctcggggaa cggggcagag gtggtcatcg ataccctgga caaggccgcc 2520 ttcaggtgtt gcatgatggg aatgagagca ggagtgatga atatgaattc catgatcttc 2580 atgaacatga agttcaatgg agagaagttt aatggggtgc tgttcatggc caacagccac 2640 atgaccctgc atggctgcag tttcttcggc ttcaacaata tgtgcgcaga ggtctggggc 2700 gcttccaaga tcaggggatg taagttttat ggctgctgga tgggcgtggt cggaagaccc 2760 aagagcgaga tgtctgtgaa gcagtgtgtg tttgagaaat gctacctggg agtctctacc 2820 gagggcaatg ctagagtgag acactgctct tccctggaga cgggctgctt ctgcctggtg 2880 aagggcacag cctctctgaa gcataatatg gtgaagggct gcacggatga gcgcatgtac 2940 aacatgctga cctgcgattc gggggtctgc catatcctga agaacatcca tgtgacctcc 3000 caccccagaa agaagtggcc agtgtttgag aataacctgc tgatcaagtg ccatatgcac 3060 ctgggagcca gaaggggcac cttccagccg taccagtgca actttagcca gaccaagctg 3120 ctgttggaga acgatgcctt ctccagggtg aacctgaacg gcatctttga catggatgtc 3180 tcggtgtaca agatcctgag atacgatgag accaagtcca gggtgcgcgc ttgcgagtgc 3240 gggggcagac acaccaggat gcagccagtg gccctggatg tgaccgagga gctgagacca 3300 gaccacctgg tgatggcctg taccgggacc gagttcagct ccagtgggga ggacacagat 3360 tagaggtagg tcgagtgagt agtgggcgtg gctaaggtga ctataaaggc gggtgtctta 3420 cgagggtctt tttgcttttc tgcagacatc atgaacggga ccggcggggc cttcgaaggg 3480 gggcttttta gcccttattt gacaacccgc ctgccgggat gggccggagt tcgtcagaat 3540 gtgatgggat cgacggtgga tgggcgccca gtgcttccag caaattcctc gaccatgacc 3600 tacgcgaccg tggggagctc gtcgctcgac agcaccgccg cagccgcggc agccgcagct 3660 gccatgacag cgacgagact ggcctcgagc tacatgccca gcagcggcag cagcccctct 3720 gtgcccagtt ccatcatcgc cgaggagaaa ctgctggccc tgctggccga gctggaagcc 3780 ctgagccgcc agctggccgc cctgacccag caggtgtccg agctccgcga gcagcaacag 3840 cagcaaaata aatgaaaact tcaataaaca cagattctga ttcaaacagc aaagcatctt 3900 tattatttat tttttcgcgc gcggtaggcc ctggtccacc tctcccgatc attgagagtg 3960 cggtggattt tttccaggac ccggtagagg tgggattgga tgttgaggta catgggcatg 4020 agcccgtccc gggggtggag gtagcaccac tgcatggcct cgtgctctgg ggtcgtgttg 4080 tagatgatcc agtcatagca ggggcgctgg gcgtggtgct ggatgatgtc tttgagaagg 4140 agactgatgg ccacggggag ccccttggtg taggtattgg cgaagcggtt gagctgggag 4200 ggatgcatgc ggggggagat gatgtgcagt ttggcctgga tcttgaggtt ggcgatgttg 4260 ccacccagat cccgccgggg gttcatgttg tgcaggacca ccaggacggt gtagcccgtg 4320 cacttgggga acttgtcatg caacttggaa gggaatgcgt ggaagaattt ggagacgccc 4380 ttgtgcccgc ccaggttttc catgcattca tccatgatga tggcgatggg cccgtgggct 4440 gcggctttgg caaagacgtt tctggggtca gatacatcat aattatgctc ctgggtgaga 4500 tcatcataag acattttaat gaatttgggg cggagggtgc cagattgggg gacgatggtt 4560 ccctcgggcc ccggggcgaa gttcccctca cagatctgca tctcccaggc tttcatctcg 4620 gaggggggga tcatgtccac ctgcggggcg atgaaaaaaa cggtttccgg ggctggggtg 4680 atgagctgcg aagagagcag gtttctcaac agctgggact tgccgcaccc ggtcgggccg 4740 tagatgaccc cgatgacggg ttgcaggtgg tagttcaagg acatgcagct gccgtcgtcc 4800 cggaggaggg gggccacctc gttgagcatg tctctgactt ggaggttttc ccgaacgagc 4860 tcgccgagga ggcggtcccc gcccagcgag aggagctctt gcagggaagc aaagtttttc 4920 aggggcttga gtccgtcggc catgggcatc ttggcgaggg tctgcgagag gagctccagg 4980 cggtcccaga gctcggtgac gtgctctacg gcatctcgat ccagcagact tcctcgtttc 5040 gggggttggg acgactgcga ctgtagggca cgagacgatg ggcgtccagc gcggccagcg 5100 tcatgtcctt ccagggtctc agggtccgag tgagggtggt ctccgtcacg gtgaaggggt 5160 gggcccctgg ctgggcgctt gcaagggtgc gcttgagact catcctgctg gtgctgaaac 5220 gggcacggtc ttcgccctgc gagtcggcga gatagcagtt gaccatgagc tcgtagttga 5280 gggcctcggc ggcgtggccc ttggcgcgga gcttgccctt ggaagagcgc ccgcaggcgg 5340 gacagaggag ggattgcagg gcgtagagct tgggggcgag aaagacggac tcgggagcga 5400 aagcgtccgc tccgcagtgg gcgcagacgg tctcgcactc gacgagccag gtgagctctg 5460 gctgctcggg gtcaaaaacc agttttcccc cgttcttttt gatgcgcttc ttacctcgcg 5520 tctccatgag tctgtgtccg cgctcggtga caaacaggct gtcggtgtcc ccgtagacgg 5580 acttgattgg cctgtcctgc aggggcgtcc cgcggtcctc ctcgtagaga aactcggacc 5640 actctgagac aaaggcgcgc gtccacgcca agacaaagga ggccacgtgc gaggggtagc 5700 ggtcgttgtc cactaggggg tccacctttt ccaccgtgtg cagacacatg tccccctcct 5760 ccgcatccaa gaaggtgatt ggcttgtagg tgtaggccac gtgaccgggg gtccccgacg 5820 ggggggtata aaagggggcg ggtctgtgct cgtcctcact ctcttccgcg tcgctgtcca 5880 cgagcgccag ctgttggggt aggtattccc tctcgagagc gggcatgacc tcggcactca 5940 ggttgtcagt ttctagaaac gaggaggatt tgatgttggc ttgccctgcc gcgatgcttt 6000 ttaggagact ttcatccatc tggtcagaaa agactatttt tttattgtca agcttggtgg 6060 cgaaggagcc atagagggcg ttggatagaa gcttggcgat ggatctcatg gtctgatttt 6120 tgtcacggtc ggcgcgctcc ttggccgcga tgtttagctg gacatactcg cgcgcgacgc 6180 acttccattc ggggaagacg gtggtgcgct cgtcgggcac gatcctgacg cgccagccgc 6240 ggttatgcag ggtgaccagg tccacgctgg tggccacctc gccgcgcagg ggctcgttgg 6300 tccagcagag tctgccgccc ttgcgcgagc agaacggggg cagcacatca agcagatgct 6360 cgtcaggggg gtccgcatcg atggtgaaga tgcccggaca gagttccttg tcaaaataat 6420 cgatttttga ggatgcatca tccaaggcca tctgccactc gcgggcggcc agcgctcgct 6480 cgtaggggtt gaggggcgga ccccagggca tgggatgagt gagggcggag gcgtacatgc 6540 cgcagatgtc gtagacatag atgggctccg agaggatgcc gatgtaggtg ggataacagc 6600 gccccccgcg gatgctggcg cgcacgtagt catacaactc gtgcgagggg gccaagaagg 6660 cggggccgag attggtgcgc tggggctgct cggcgcggaa gacgatctgg cgaaagatgg 6720 cgtgcgagtt ggaggagatg gtgggccgtt ggaagatgtt aaagtgggcg tggggcaagc 6780 ggaccgagtc gcggatgaag tgcgcgtagg agtcttgcag cttggcgacg agctcggcgg 6840 tgacgaggac gtccatggcg cagtagtcca gcgtttcgcg gatgatgtca taacccgcct 6900 ctcctttctt ctcccacagc tcgcggttga gggcgtactc ctcgtcatcc ttccagtact 6960 cccggagcgg gaatcctcga tcgtccgcac ggtaagagcc cagcatgtag aaatggttca 7020 cggccttgta gggacagcag cccttctcca cggggagggc gtaagcttga gcggccttgc 7080 ggagcgaggt gtgcgtcagg gcgaaggtgt ccctgaccat gactttcaag aactggtact 7140 tgaaatccga gtcgtcgcag ccgccgtgct cccagagctc gaaatcggtg cgcttcttcg 7200 agagggggtt aggcagagcg aaagtgacgt cattgaagag aatcttgcct gctcgcggca 7260 tgaaattgcg ggtgatgcgg aaagggcccg ggacggaggc tcggttgttg atcacctggg 7320 cggcaaggac gatctcgtcg aagccgttga tgttgtgccc gacgatgtag agttccatga 7380 atcgcgggcg gcctttgatg tgcggcagct ttttgagctc ctcgtaggtg aggtcctcgg 7440 ggcattgcag gccgtgctgc tctagcgccc actcctggag atgtgggttg gcttgcatga 7500 aggaagccca gagctcgcgg gccatgaggg tctggagctc gtcgcgaaag aggcggaact 7560 gctggcccac ggccatcttt tctggggtga cgcagtagaa ggtgaggggg tcccgctccc 7620 agcgatccca gcgtaagcgc acggcgagat cgcgagcgag ggcgaccagc tcgggttccc 7680 ccgagaattt catgaccagc atgaagggga cgagctgctt gccgaaggac cccatccagg 7740 tgtaggtttc tacatcgtag gtgacaaaga gccgctccgt gcgaggatga gagccgattg 7800 ggaagaactg gatttcctgc caccagttgg acgagtggct gttgatgtga tgaaagtaga 7860 aatcccgccg gcgaaccgag cactcgtgct gatgcttgta aaagcgtccg cagtactcgc 7920 agcgctgcac gggctgtacc tcatccacga gatacacagc gcgtcccttg aggaggaact 7980 tcaggagtgg cggccctggc tggtggcttt catgttcgcc tgcgtgggac tcaccctggg 8040 gctcctcgag gacggagagg ctgacgagcc cgcgcgggag ccaggtccag atctcggcgc 8100 ggcgggggcg gagagcgaag acgagggcgc gcagttggga gctgtccatg gtgtcgcgga 8160 gatccaggtc cgggggcagg gttctgaggt tgacctcgta gaggcgggtg agggcgtgct 8220 tgagatgcag atggtacttg atctccacgg gtgagttggt ggtcgtgtcc acgcattgca 8280 tgagcccgta gctgcgcggg gccacgaccg tgccgcggtg cgcttttaga agcggtgtcg 8340 cgggcgtgct cccggcggca gcggcggttc tggccccgcg ggcaggggcg gcagaggcac 8400 gtcggcgtgg cgctcgggca ggtcccggtg ctgcgccctg agagcgctgg cgtgcgcgac 8460 gacgcggcgg ttgacatcct ggatctgccg cctctgcgtg aagaccacgg gccccgtgac 8520 tttgaacctg aaagacagtt caacagaatc aatctctgcg tcattgacgg cggcctgacg 8580 caggatctct tgcacgtcgc ccgagttgtc ctggtaggcg atctcggtca tgaactgttc 8640 gatctcctcc tcctggagat cgccgcggcc cgcgcgctcc acggtggcgg cgaggtcatt 8700 ggagatgcgg cccatgagct gcgagaaggc gcccaggccg ctctcgttcc agacgcggct 8760 gtagaccacg tccccgtcgg cgtcgcgtgc gcgcatgacc acctgcgcga ggttgagctc 8820 cacgtgccgc gtgaagacgg cgtagttgcg caggcgctgg aagaggtagt tgagggtggt 8880 ggcgatgtgc tcggtgacga agaagtacat gatccagcgg cgcaggggca tctcgctgat 8940 gtcgccgatg gcctccagcc tttccatggc ctcgtagaaa tccacggcga agttgaaaaa 9000 ctgggcattg cgggccgaga ccgtgagctc gtcttccagg agccggatga gctcggcgat 9060 ggtggcgcgc acctcgcgct cgaaatcccc gggggcctcc tcttcttcct cttcttccat 9120 gacgacctct tcttctattt cttcctctgg gggcggtggt ggtggcgggg cccgacgacg 9180 acggcgacgc accgggagac ggtcgacgaa gcgctcgatc atctccccgc ggcggcgacg 9240 catggtttcg gtgacggcgc gaccccgttc gcgaggacgc agcgtgaaga cgccgccggt 9300 catctcccgg taatggggcg ggtccccgtt gggcagcgat agggcgctga cgatgcatct 9360 tatcaattgc ggtgtagggg acgtgagcgc gtcgagatcg accggatcgg agaatctttc 9420 gaggaaagcg tctagccaat cgcagtcgca aggtaagctc aaacacgtag cagccctgtg 9480 gacgctgtta gaattgcggt tgctgatgat gtaattgaag taggcgtttt tgaggcggcg 9540 gatggtggcg aggaggacca ggtccttggg tcccgcttgc tggatgcgga gccgctcggc 9600 catgccccag gcctggccct gacaccggct caggttcttg tagtagtcat gcatgagcct 9660 ctcgatgtca tcactggcgg aggcggagtc ttccatgcgg gtgaccccga cgcccctgag 9720 cggctgcacg agcgccaggt cggcgacgac gcgctcggcg aggatggcct gttgcacgcg 9780 ggtgagggtg tcctggaagt cgtccatgtc gacaaagcgg tggtaggccc cggtgttgat 9840 ggtgtaggtg cagttggcca tgagcgacca gttgacggtc tgcaggccgg gttgcacgac 9900 ctcggagtac ctgagccgcg agaaggcgcg cgagtcgaag acgtagtcat tgcaggtgcg 9960 cacgaggtac tggtagccga ctaggaagtg cggcggcggc tggcggtaga gcggccagcg 10020 ctgggtggcc ggcgcgcccg gggccaggtc ctcgagcatg aggcggtggt agccgtagag 10080 gtagcgggac atccaggtga tgccggcggc ggtggtggag gcgcgcggga actcgcggac 10140 gcggttccag atgttgcgca gcggcaggaa atagtccatg gtcggcacgg tctggccggt 10200 gagacgcgcg cagtcattga cgctctagag gcaaaaacga aagcggttga gcgggctctt 10260 cctccgtagc ctggcggaac gcaaacgggt taggccgcgt gtgtaccccg gttcgagtcc 10320 ccttgaatca ggctggagcc gcgactaacg tggtattggc actcccgtct cgacccgagc 10380 ccgatagccg ccaggatacg gcggagagtc ctttttgccg gccaaggggg gtcgctagac 10440 ttgaaagcga ccgaaaaccc tgccgggtag tggctcgcgc ccgtagtctg gagaagcatc 10500 gccagggttg agtcgcggca gaacccggtt cgcggacggc cgcggcgagc gggacttggt 10560 caccccgccg atttaaagac ccacagccag ccgacttctc cagttacggg agcgagcccc 10620 cttttttctt tttgccagat gcatcccgtc ctgcgccaaa tgcgtcccac ccccccggcg 10680 accaccgcga ccgcggccgt agcaggcgcc ggcgctagcc agccacagcc acagacagag 10740 atggacttgg aagagggcga agggctggcg agactggggg cgccgtcccc ggagcgacac 10800 ccccgcgtgc agctgcagaa ggacgtgcgc ccggcgtacg tgcctgcgca gaacctgttc 10860 agggaccgca gcggggagga gcccgaggag atgcgcgact gccggtttcg ggcgggcagg 10920 gagctgcgcg agggcctgga ccgccagcgc gtgctgcgcg acgaggattt cgagccgaac 10980 gagcagacgg ggatcagccc cgcgcgcgct cacgtggcgg cggccaacct ggtgacggcc 11040 tacgagcaga cggtgaagca ggagcgcaac ttccaaaaga gtttcaacaa ccacgtgcgc 11100 accctgatcg cgcgcgagga ggtggccctg ggcctgatgc acctgtggga cctggcggag 11160 gccatcgtgc agaacccgga cagcaagcct ctgacggcgc agctgttcct ggtggtgcag 11220 cacagcaggg acaacgaggc gttcagggag gcactgctga acatcgccga gcccgagggt 11280 cgctggctgc tggagctgat caacatcttg cagagcatcg tagtgcagga gcgcagcctg 11340 agcttggccg agaaggtggc ggcgatcaac tactcggtgc tgagcctggg caagttttac 11400 gcgcgcaaga tttacaagac gccgtacgtg cccatagaca aggaggtgaa gatagacagc 11460 ttttacatgc gcatggcgct caaggtgctg acgctgagcg acgacctggg cgtgtaccgc 11520 aacgaccgca tccacaaggc cgtgagcacg agccggcggc gcgagctaag cgaccgcgag 11580 ctgatgctga gcctgcgccg ggcgctggta gggggcgctg ccggcggcga ggagtcctac 11640 ttcgacatgg gggcggacct gcattggcag ccgagccggc gcgccttgga ggccgcctac 11700 ggtccagagg acttggatga ggaagaggaa gaggaggagg atgcacccgc tgcggggtac 11760 tgacgcctcc gtgatgtgtt tttagatgca gcaagccccg gaccccgcca taagggcggc 11820 gctgcaaagc cagccgtccg gtctagcatc ggacgactgg gaggccgcga tgcaacgcat 11880 catggccctg acgacccgca accccgagtc ctttagacaa cagccgcagg ccaacagact 11940 ctcggccatt ctggaggcgg tggtcccctc tcggaccaac cccacgcacg agaaggtgct 12000 ggcgatcgtg aacgcgctgg cggagaacaa ggccatccgt cccgacgagg ccgggctggt 12060 gtacaacgcc ctgctggagc gcgtgggccg ctacaacagc acgaacgtgc agtccaacct 12120 ggaccggctg gtgacggacg tgcgcgaggc cgtggcgcag cgcgagcggt tcaagaacga 12180 gggcctgggc tcgctggtgg cgctgaacgc cttcctggcg acgcagccgg cgaacgtgcc 12240 gcgcgggcag gacgattaca ccaactttat cagcgcgctg cggctgatgg tgaccgaggt 12300 tccccagagc gaggtgtacc agtcgggccc ggactacttt ttccagacga gccggcaggg 12360 cttgcagacg gtgaacctga gccaggcttt caagaacctg cgagggctgt ggggcgtgca 12420 ggcgcccgtg ggcgaccggt cgacggtgag cagcttgctg acgcccaact cgcggctgct 12480 gctgctgctg atcgcgccct tcaccgacag cggcagcgtg aaccgcaact cgtacctggg 12540 ccatctgctg acgctgtacc gcgaggccat aggccaggcg caggtggacg agcagacctt 12600 ccaggagatc actagcgtga gccgcgcgct ggggcagaac gacaccgaca gtctgagggc 12660 caccctgaac ttcttgctga ccaatagaca gcagaagatc ccggcgcaat atgcgctgtc 12720 ggccgaggag gaaaggatcc tgagatatgt gcagcagagc gtagggctgt tcctgatgca 12780 ggagggggcc acccccagcg ccgcgctgga catgaccgcg cgcaacatgg aacctagcat 12840 gtacgccgcc aaccggccgt tcatcaataa gctgatggac tacctgcacc gcgcggcggt 12900 catgaacacg gactacttta caaacgccat cctgaacccg cactggctcc cgccgccggg 12960 gttctacacg ggcgagtacg acatgcccga ccccaacgac gggttcctgt gggacgacgt 13020 ggacagcgtg gtgttctcgc cgacctttca aaagcgccag gaggcgccgc cgagcgaggg 13080 cgcggtgggg aggagcccct ttcctagctt agggagtttg catagcttgc cgggctcggt 13140 gaacagcggc agggtgagcc ggccgcgctt gctgggcgag gacgagtacc tgaacgactc 13200 gctgctgcag ccgccgcggg ccaagaacgc catggccaat aacgggatag agagtctggt 13260 ggacaaactg aaccgttgga agacctacgc tcaggaccat agggatgcgc ccgcgccgcg 13320 gcgacagcgc cacgaccggc agcggggcct ggtgtgggac gacgaggact cggccgacga 13380 tagcagcgtg ttggacttgg gcgggagcgg tggggtcaac ccgttcgcgc atctgcagcc 13440 caaactgggg cgacggatgt tttgaaatgc aaaataaaac tcaccaaggc catagcgtgc 13500 gttctcttcc ttgttagaga tgaggcgtgc ggtggtgtct tcctctcctc ctccctcgta 13560 cgagagcgtg atggcgcagg cgaccctgga ggttccgttt gtgcctccgc ggtatatggc 13620 tcctacggag ggcagaaaca gcattcgtta ctcggagctg gctccgcagt acgacaccac 13680 tcgcgtgtac ttggtggaca acaagtcggc ggacatcgct tccctgaact accaaaacga 13740 ccacagcaac ttcctgacca cggtggtgca gaacaacgat ttcacccccg ccgaggccag 13800 cacgcagacg ataaattttg acgagcggtc gcggtggggc ggtgatctga agaccattct 13860 gcacactaac atgcccaatg tgaacgagta catgttcacc agcaagttta aggcgcgggt 13920 gatggtggct aggaagcatc cagagggggt agttgaaaca gatttgagtc aggataagct 13980 tgaatatgag tggtttgagt ttaccctgcc cgagggaaac ttttccgaga ccatgaccat 14040 agacctgatg aacaacgcca tcttggaaaa ctacttgcaa gtggggcggc aaaatggcgt 14100 gctggagagc gatatcggag tcaagtttga cagcagaaat ttcaagctgg gctgggaccc 14160 ggtgaccaag ctggtgatgc caggggtcta cacctacgag gccttccacc cggacgtggt 14220 gctgctgccg ggctgcgggg tggacttcac cgagagccgc ctgagcaacc tcctgggcat 14280 tcgcaagaag caacctttcc aagagggctt cagaatcatg tatgaggatc tagaaggtgg 14340 caacatcccc gccctccttg atgtgcccaa gtacttggaa agcaagaaga aagttgaaga 14400 cgaaactaaa aatgcagctg cggctacagc cgatacaacc actaggggtg atacatttgc 14460 aactccagcg caagagacag cagctgataa gaaggtagaa gtcttgccca ttgaaaagga 14520 tgagagtggt agaagttaca acctgatcca ggggacccac gacacgctgt accgcagttg 14580 gtacctgtcc tatacctacg gggaccccga gaagggggtg cagtcgtgga cgctgctcac 14640 caccccggac gtcacctgcg gcgcggagca agtctactgg tcgctgccgg acctcatgca 14700 agacccagtc accttccgct ctacccagca agtcagcaac taccccgtgg tcggcgccga 14760 gctcatgccc ttccgcgcca agagctttta caacgacctc gccgtctact cccagctcat 14820 ccgcagctac acctccctca cccacgtctt caaccgcttc cccgacaacc agatcctctg 14880 ccgcccgccc gcgcccacca tcaccaccgt cagtgaaaac gtgcctgctc tcacagatca 14940 cgggacgcta ccgctgcgca gcagtatccg cggagtccag cgagtgaccg tcactgacgc 15000 ccgtcgccgc acctgtccct acgtctacaa ggccctgggc atagtcgcgc cgcgcgtgct 15060 ttccagtcgc accttctaaa aaaatgtcta ttctcatctc gcccagcaat aacaccggct 15120 ggggtcttac tagacccagc accatgtacg gaggagccaa gaagcgctcc cagcagcacc 15180 ccgtccgcgt ccgcggccac ttccgcgctc cctggggcgc ttacaagcgc gggcggactt 15240 ccaccgccgt gcgcaccacc gtcgacgacg tcatcgactc ggtggtcgcc gacgcgcgca 15300 actatacccc cgccccctcc accgtggacg cggtcatcga cagcgtggtg gccgacgcgc 15360 gcgactatgc cagacgcaag agccggcggc gacggatcgc caggcgccac cggagcacgc 15420 ccgccatgcg cgccgcccgg gctctgctgc gccgcgccag acgcacgggc cgccgggcca 15480 tgatgcgagc cgcgcgccgc gctgccactg cacccacccc cgcaggcagg actcgcagac 15540 gagcggccgc cgccgccgcc gcggccatct ctagcatgac cagacccagg cgcggaaacg 15600 tgtactgggt gcgcgactcc gtcacgggcg tgcgcgtgcc cgtgcgcacc cgtcctcctc 15660 gtccctgatc taatgcttgt gtcctccccc gcaagcgacg atgtcaaagc gcaaaatcaa 15720 ggaggagatg ctccaggtcg tcgccccgga gatttacgga ccaccccagg cggaccagaa 15780 accccgcaaa atcaagcggg ttaaaaaaaa ggatgaggtg gacgaggggg cagtagagtt 15840 tgtgcgcgag ttcgctccgc ggcggcgcgt aaattggaag gggcgcaggg tgcagcgcgt 15900 gttgcggccc ggcacggcgg tggtgttcac gcccggcgag cggtcctcag tcaggagcaa 15960 gcgtagctat gacgaggtgt acggcgacga cgacatcctg gaccaggcgg cggagcgggc 16020 gggcgagttc gcctacggga agcggtcgcg cgaagaggag ctgatctcgc tgccgctgga 16080 cgaaagcaac cccacgccga gcctgaagcc cgtgaccctg cagcaggtgc tgccccaggc 16140 ggtgctgctg ccgagccgcg gggtcaagcg cgagggcgag agcatgtacc cgaccatgca 16200 gatcatggtg cccaagcgcc ggcgcgtgga ggacgtgctg gacaccgtga aaatggatgt 16260 ggagcccgag gtcaaggtgc gccccatcaa gcaggtggcg ccgggcctgg gcgtgcagac 16320 cgtggacatt cagatcccca ccgacatgga tgtcgacaaa aaaccctcga ccagcatcga 16380 ggtgcagacc gacccctggc tcccagcctc cactgctacc gtctccactt ctaccgccgc 16440 cacggctacc gagcctccca ggaggcgaag atggggcgcc gccagccggc tgatgcccaa 16500 ctacgtggtg catccttcca tcatcccgac gccgggctac cgcggcaccc ggtactacgc 16560 cagccgccgg cgcccagcca gcaaacgccg ccgccgcacc gccacccgcc gccgtctggc 16620 ccccgcccgc gtgcgccgcg tgaccacgcg ccggggccgc tcgctcgttc tgcccaccgt 16680 gcgctaccac cccagcatcc tttaattcgt gtgctgtgat actgttgcag agagatggct 16740 ctcacttgcc gcctgcgcat ccccgtcccg aattaccgag gaagatcccg ccgcaggaga 16800 ggcatggcag gcagcggcct gaaccgccgc cggcggcggg ccatgcgcag gcgcctgagt 16860 ggcggctttc tgcccgcgct catccccata atcgccgcgg ccatcggcac gatcccgggc 16920 atagcttccg ttgcgctgca ggcgtcgcag cgccgttgat gtgcgaataa aagcctcttt 16980 agactctgac acacctggtc ctgtatattt ttagaatgga agacatcaat tttgcgtccc 17040 tggctccgcg gcacggcacg cggccgttca tgggcacctg gaacgagatc ggcaccagcc 17100 agctgaacgg gggcgccttc aattggagca gtgtctggag cgggcttaaa aatttcggct 17160 cgacgctccg gacctatggg aacaaggcct ggaatagtag cacggggcag ttgctaaggg 17220 aaaagctcaa agaccagaac tttcagcaga aggtggtgga cgggctggcc tcgggcatta 17280 acggggtggt ggacatcgcg aaccaggccg tgcagcgcga gataaacagc cgcctggacc 17340 cgcggccgcc cacggtggtg gagatggaag atgcaactct tccgccgccc aaaggcgaga 17400 agcggccgcg gcccgacgcg gaggagacga tcctgcaggt ggacgagccg ccctcgtacg 17460 aggaggccgt caaggccggc atgcccacca cgcgcatcat cgcgccgctg gccacgggtg 17520 taatgaaacc cgccaccctt gacctgcctc caccacccgc gcccgctcca ccgaaggcag 17580 ctcccgcggt cgtgcaggcc cccccggtgg cgaccgccgt gcgccgcgtc cccgcccgcc 17640 gccaggccca gaactggcag agcacgttgc acagtatcgt aggcctggga gtgaaaagtc 17700 tgaagcgccg ccgatgctat tgaaagagag gaaagaggac actaaaggga gagcttaact 17760 tgtatgtgcc ttaccgccag agaacgcgcg aagatggcca ccccctcgat gatgccgcag 17820 tgggcgtaca tgcacatcgc cgggcaggac gcctcggagt acctgagccc gggtctggtg 17880 cagtttgccc gcgccaccga cacgtacttc agcctgggca acaagtttag gaaccccacg 17940 gtggccccga cccacgatgt gaccacggac cggtcccagc gtctgacgct gcgcttcgtg 18000 cccgtggatc gcgaggacac cacgtactcg tacaaggcgc gcttcactct ggccgtgggc 18060 gacaaccggg tgctagacat ggccagcact tactttgaca tccgcggcgt cctggaccgc 18120 ggtcccagct tcaaacccta ctcgggcaca gcttacaaca gtctggcccc caagggtgcc 18180 cccaactcca gccagtggct tgcaaaagac accaatgctg gcgatcaagc attaaaaacc 18240 cacacacatg gcgtagctgc tatgggggga acagatatca cagcaaaggg tttgcaaatt 18300 ggtgttgaca cgactgaaaa caagaatgag cctatttatg caaatgaaat ataccagcca 18360 gaacctcagg taggagagga aaacttgcaa gatgttgaaa acttttatgg aggcagagct 18420 cttaaaaaag aaaccaaaat gaaaccttgc tatggctcgt ttgccagacc cacaaatgaa 18480 aaaggcggtc aagccaaatt tttaactgac ggcgatggtc agctaactaa aaatcatgat 18540 atcacaatga atttctttga cactcctgga ggaacagttg gtcaggatac tgaacttgaa 18600 gcagacattg ttatgtatgc tgagaatgtg catctggaaa ctccagacac gcatgtggtg 18660 tacaaaccag gaacttctga tgagagttca gaagcaaatt tggttcagca gtccatgcca 18720 aacaggccca actacatcgg cttcagggac aactttgtgg gtctcatgta ctataacagc 18780 actggcaaca tgggtgtgct ggctggtcaa gcatctcagt tgaatgctgt ggtcgacttg 18840 caagacagaa acacagagct gtcttaccag ctcttgctag attctctggg tgacagaacc 18900 agatacttta gcatgtggaa ctctgcagtg gacagttatg atcctgatgt caggattatt 18960 gaaaatcacg gtgtggaaga tgaacttcca aactattgct tcccattgga tggagctggc 19020 actaatgcta cctaccaagg tgtaaaagtt aaaaatggcc aagatggaga tgtaaacgca 19080 gattgggaaa aagatccaaa tcttgcttca cgaaaccaaa tatgcaaggg taacatcttc 19140 gccatggaga tcaacctcca ggccaacctg tggaagagtt ttctgtactc gaatgtggcc 19200 ctgtacctgc ccgactcata caagtacacg ccggccaacg tcacgctgcc cgccaatacc 19260 aacacctacg agtacatgaa cggccgcgtg gtagccccct cgctggtgga cgcctacatc 19320 aacatcggcg cccggtggtc gctggacccc atggacaacg tcaacccatt caaccaccac 19380 cgcaacgcgg gcctgcgtta ccgctccatg cttctgggca acggccgcta cgtgcccttc 19440 cacatccaag tgccccaaaa gttctttgcc atcaagaacc tgctcctgct ccccggctcc 19500 tacacctacg agtggaactt ccgcaaggat gtcaacatga tcctgcagag ttccctcgga 19560 aacgacctgc gcgtcgacgg cgcctccgtc cgcttcgaca gcgtcaacct ctacgccaca 19620 ttcttcccca tggcgcacaa caccgcctcc accctggaag ccatgctgcg caacgacacc 19680 aacgaccagt ccttcaacga ctacctctcg gccgccaaca tgctctaccc catcccggcc 19740 aaggccacca acgtgcccat ctccatcccc tcgcgcaact gggccgcctt ccgcggctgg 19800 agtttcaccc ggctcaagac caaagaaact ccctccctcg gctcgggttt cgatccctac 19860 tttgtatact cgggttccat cccctacctc gacgggacct tctacctcaa ccacaccttc 19920 aagaaggtct ccatcatgtt cgactcctcg gtcagctggc ccggcaacga ccggctgctc 19980 acgccgaacg agttcgagat caagcgcagt gtcgacgggg agggctacaa tgtggcccaa 20040 tgcaacatga ccaaggactg gttcctcgtc cagatgctct cccactacaa catcggctac 20100 cagggcttcc acgtgcccga gggctacaag gaccgcatgt actccttctt ccgcaacttc 20160 cagcccatga gcaggcaggt ggtcgatgag atcaactaca aggactacaa ggccgtcacc 20220 ctgcccttcc agcacaacaa ctcgggcttc accggctacc ttgcacccac catgcgtcag 20280 gggcagccct accccgccaa cttcccctat cctctcatcg gccagacagc cgtgccctct 20340 gtcacccaga aaaagttcct ctgcgacagg gtcatgtggc gcatcccctt ctccagcaac 20400 ttcatgtcca tgggcgccct caccgacctg ggtcagaaca tgctctatgc caactcggcc 20460 cacgcgctcg acatgacctt cgaggtggac cccatggatg agcccaccct cctctatctt 20520 ctcttcgaag ttttcgacgt ggtcagagtg caccagccgc accgcggcgt catcgaggcc 20580 gtctacctgc gcacgccctt ctccgccggc aacgccacca cctaagcatg agcggctcca 20640 gcgaacgaga gctcgcggcc atcgtgcgcg acctgggctg cgggccctac tttttgggca 20700 cccacgacaa gcgcttcccg ggcttcctcg ccggcgacaa gctggcctgc gccattgtca 20760 acacggccgg ccgcgagacc ggaggcgtgc actggctcgc cttcggctgg aacccgcgct 20820 cgcgcacctg ctacatgttc gacccatttg ggttctcgga ccgccggctc aagcagattt 20880 acagcttcga gtacgaggct atgttgcgcc gaagcgccct ggcttcctcg cccgaccgct 20940 gtctcagcct cgagcagtcc acccagaccg tgcaggggcc cgactccgcc gcctgcggac 21000 ttttctgttg catgttcttg catgccttcg tgcactggcc cgaccgaccc atggacggga 21060 accccaccat gaacttgctg acgggggtgc ccaacggcat gctacaatcg ccacaggtgc 21120 tgcccaccct ccggcgcaac caggaggagc tctaccgctt cctcgcgcgc cactcccctt 21180 actttcgctc ccaccgcgcc gccatcgaac acgccaccgc ttttgacaaa atgaaacaac 21240 tgcgtgtatc tcaataaaca gcacttttat tttacatgta ctggagtata tgcaagttat 21300 ttaaaagtcg aaggggttct cgcgctcgtc gttgtgcgcc gcgctgggga gggccacgtt 21360 gcggtactgg aacttgggct gccacttgaa ctcggggatc accagtttgg gcactggggt 21420 ctcggggaag gtctcgctcc acatgcgccg gctcatctgc agggcgccca gtatgtccgg 21480 ggcggagatc ttgaaatcgc agttggggcc ggtgctctgc gcgcgcgagt tgcggtacac 21540 ggggttgcag cactggaaca ccatcagact ggggtacttc acactggcca gcacgctctt 21600 gtcgctgatc tgatccttgt ccaggtcctc ggcgttgctc aggccgaacg gggtcatctt 21660 gcacagctgg cggcccagga agggcacgct ctgaggcttg tggttacact cgcagtgcac 21720 gggcatcagc atcatccccg cgccgcgctg catattcggg tagagggcct tgacaaaggc 21780 cgagatctgc ttgaaagctt gctgggcctt ggccccctcg ctgaaaaaca ggccgcagct 21840 cttcccgctg aactggttat tcccgcaccc ggcatcatgc acgcagcagc gcgcgtcatg 21900 gctggtcagt tgcaccacgc tccgtcccca gcggttctgg gtcaccttgg ccttgctagg 21960 ctgctccttc aacgcgcgct ggccgttctc gctggtcaca tccatctcca ccacgtggtc 22020 cttgtggatc atcaccgtcc cgtgcaaaca cttgagctgg ccttccacct cggtgcagcc 22080 gtgatcccac agggcgcagc cggtgcactc ccaattcttg tgtgcgatcc cgctgtggct 22140 gaagatgtaa ccttgcaaca ggcgacccat gacggtgcta aatgatttac tggtgctgaa 22200 ggtcagttgc agaccgcggg cctcctcgtt catccaggtc tggcacatct tttggaagat 22260 ctcggtctgc tcgggcatga gcttgtaagc atcgcgcagg ccgctgtcga cgcggtagcg 22320 ttccataagc acgttcatgg tatccatgcc cttctcccag gacgagacca gaggcagact 22380 cagggggttg cgcacgttca gaataccggg ggtcgcgggc tcgacgatgc gttttccgtc 22440 cttgccttcc ttcaacagaa ccggcggctg gctgaatccc actcccacga tcacggcatc 22500 ttcctggggc atctcttcgt cggggtctac cttggtcaca tgcttggtct ttctggcttg 22560 cttctttttt ggagggctgt ccacggggac cacgtcctcc tcggaagacc cggagcccac 22620 ccgctgatac tttcggcgct tggtgggcag aggaggtgga ggcgaggggc tcctctcctg 22680 ctccggcgga tagcgcgccg acccgtggcc ccggggcgga gtggcctctc gctccatgaa 22740 ccggcgcacg tcctgactgc cgccggccat tgtttcctag gggaagatgg aggagcagcc 22800 gcgtaagcag gagcaggagg aggacttaac cacccacgag caacccaaaa tcgagcagga 22860 cctgggcttc gaagagccgg ctcgtctaga acccccacag gatgaacagg agcacgagca 22920 agacgcaggc caggaggaga ccgacgctgg gctcgagcat ggctacctgg gaggagagga 22980 ggatgtgctg ctgaaacact tgcagcgcca gtccctcatc ctccgggacg ccctggccga 23040 ccggagcgaa acccccctca gcgtcgagga gctgtgtcgg gcctacgagc tcaacctctt 23100 ctcgccgcgc gtgcccccca aacgccagcc caacggcacc tgcgagccca acccgcgtct 23160 caacttctat cccgttttcg cggtccccga ggcccttgcc acctatcaca tttttttcaa 23220 gaaccaaaag atccccgtct cctgccgcgc caaccgcacc cgcgccgacg cgctcctcgc 23280 tctggggccc ggcgcgcgca tacctgatat cgcttccctg gaagaggtgc ccaagatctt 23340 cgaagggctc ggtcgggacg agacgcgcgc ggcgaacgct ctgaaagaaa cagcagagga 23400 agagggtcac actagcgccc tggtagagtt ggaaggcgac aacgccaggc tggccgtgct 23460 caagcgcagc gtcgagctca cccactttgc ctaccccgcc gtcaacctcc cgcccaaggt 23520 catgcgtcac atcatggatc agctcatcat gccccacatc gaagccctcg atgaaagtca 23580 ggagcagcgc cccgaggacg cccgacccgt ggtcagcgac gagatgctcg cgcgctggct 23640 cgggacccgc gagccccagg ctttggaaca gcggcgcaag ctcatgctgg ccgtggtcct 23700 ggtcaccctc gagctcgaat gcatgcgccg cttcttcagc gaccccgaga ccctgcgcaa 23760 agtcgaggag accctgcact acactttcag acacggtttc gtcaggcagg cctgcaagat 23820 ttccaacgtg gagctgacca acctggtctc ctgcctgggg atcctgcatg agaaccgcct 23880 gggacagacc gtactccact ctaccctgaa gggcgaggcg cggagggact atgtccgcga 23940 ctgcgtcttt ctctttctct gccacacatg gcaagcagcc atgggcgtgt ggcagcagtg 24000 tctcgaggac gagaacctga aggagctgga caagcttctt gctagaaacc ttaaaaagct 24060 ttggacgggc ttcgacgagc gcaccgtcgc ctcggacctg gccgagatcg tcttccccga 24120 gcgcctgagg cagacgctga aaggcgggct gcccgacttc atgagccaga gcatgttgca 24180 aaactaccgc actttcattc tcgagcgatc ggggatgctg cccgccacct gcaacgcttt 24240 cccctccgac tttgtcccgc tgagctaccg cgagtgtccc ccgccgctgt ggagccactg 24300 ctacctcttg cagctggcca actacatcgc ctaccactcg gacgtgatcg aggacgtgag 24360 cggcgagggg ctgctcgagt gccactgccg ctgcaacctg tgctccccgc accgctccct 24420 ggtctgcaac ccccagctcc ttagcgagac ccaggtcatc ggtaccttcg agctgcaagg 24480 tccgcaggag tccaccgctc cgctgaaact cacgccgggg ttgtggactt ccgcgtacct 24540 gcgcaaattt gtacccgagg actaccacgc ccatgagata aagttcttcg aggaccaatc 24600 gcggccgcag cacgcggatc tcacggcctg cgtcatcacc cagggcgcga tcctcgccca 24660 attgcacgcc atccaaaaat cccgccaaga gtttcttctg aaaaagggta gaggggtcta 24720 cctggacccc cagacgggcg aggtgctcaa cacgggtctc ccccagcatg ccgaggaaga 24780 agcaggagcc gctagtggag gagatggaag aagaatggga cagccaggca gaggaggacg 24840 aatgggagga ggagacagag gaggaagaat tggaagaggt ggaagaggag caggcaacag 24900 agcagcccgt cgccgcacca tccgcgccgg cagccccggc ggtcacggat acaacctccg 24960 cagctccggc caagcctcct cgtagatggg atcgagtgaa gggcgacggt aagcacgagc 25020 ggcagggcta ccgatcatgg agggcccaca aagccgcgat catcgcctgc ttgcaagact 25080 gcggggggaa catcgctttc gcccgccgct acctgctctt ccaccgcggg gtgaacatcc 25140 cccgcaacgt gttgcattac taccgtcacc ttcacagcta agaaaaagca agtaagagaa 25200 gtcgccggag gaggcctgag gatcgcggcg aacgagccct tgaccaccag ggagctgagg 25260 aaccggatct tccccactct ttatgccatt tttcagcaga gtcgaggtca gcagcaagag 25320 ctcaaagtaa aaaaccggtc tctgcgctcg ctcacccgca gttgcttgta ccacaaaaac 25380 gaagatcagc tgcagcgcac tctcgaagac gccgaggctc tgttccacaa gtactgcgcg 25440 ctcactctta aagactaagg cgcgcccacc cggaaaaaag gcgggaatta cctcatcgcc 25500 accttaatga gcaaggagat tcccacccct tacatgtgga gctatcagcc ccagatgggc 25560 ctggccgcgg gcgcctccca agactactcc acacgcatga actggctcag tgccggcccc 25620 tcgatgatat cacgggtcaa cggggtccgc agtcatcgaa accagatatt gttggagcag 25680 gcggcggtca cctccacgcc cagggcaaag ctcaacccgc gtaattggcc ctccaccctg 25740 gtgtatcagg aaatccccgg gccgactacc gtactacttc cgcgtgacgc actggccgaa 25800 gtccgcatga ctaactcagg tgtccagctg gccggcggcg cttcccggtg cccgctccgc 25860 ccacaatcgg gtataaaaac cctggtgatc cgaggcagag gcacacagct caacgacgag 25920 ttggtgagct cttcgatcgg tctgcgaccg gacggagtgt tccaactagc cggagccggg 25980 agatcgtcct tcactcccaa ccaggcctac ctgaccttgc agagcagctc ttcggagcct 26040 cgctcgggag gcatcggaac cctccagttc gtggaggagt ttgtgccctc ggtctacttc 26100 aaccccttct cgggatcgcc aggcctctac ccggacgagt tcataccgaa cttcgacgca 26160 gtgagagaag cggtggacgg ctacgactga atgtcccatg gtgactctgc tgagctcgct 26220 cggttgaggc atctggacca ctgccgccgc ctgcgctgct tcgcccggga gagctgcgga 26280 ctcatctact ttgagtttcc cgaggagcac cccaacggtc ctgcacacgg agtgcggatc 26340 accgtagagg gcaccaccga gtctcacctg gtcaggttct tcacccagca acccttcctg 26400 gtcgagcggg accggggcgc caccacctac accgtctact gcatctgtcc gaccccgaag 26460 ttgcatgaga atttttgctg tactctttgt ggtgagttta ataaaagcta aactcttgca 26520 atactctgga ccttgtcgtc atcaactcaa cgagaccgtc tacctcacca accagactga 26580 ggtaaaactc acctgcagac cacacaagac ctatatcatc tggttcttcg agaacacctc 26640 atttgcagtc tcaaacactc actgcaacga cggtgttgaa cttccaaaca acctttccag 26700 tggactgagt tacgatacac atagagctaa gctcgtcctc tacaatcctt tcgtagaggg 26760 aacctaccac tgccagagcg gaccttgctt ccacagtttt actttggtga acgttaccgg 26820 cagcagcaca gccgctccag aacctaacct tccttctgat actatcaaac ctcgtttcgg 26880 aggtgagcta aggcttcccc cttctgagga gggggttagc ccatacgaag tggtcgggta 26940 tttgatttta ggggtggtcc tgggtgggtg catagcggtg ctagctcagc tgccttgctg 27000 ggtagaaatc aaaatcttta tatgctgggt cagacattgt ggggaggaac tatgaagggg 27060 ctcttgctga ttatcctttc cctggtgggg ggtgtgctgt catgccacga acagccacga 27120 tgtaacatca ccacaggcaa tgagaggagt gtcatatgca cagtagtcat caaatgcgag 27180 catgaatgcc ctctcaacat cacattcaaa aaccgtacca tggggaatgc atgggtgggc 27240 gactgggaac caggagatga gcagaactac acggtcactg tccatggtag cgatggaaat 27300 cacactttcg gtttcaaatt catttttgaa gtcatgtgtg atatcacact gcatgtggct 27360 agacttcatg gcttgtggcc ccctaccaag gaaaacatgg ttgggttttc tttggctttt 27420 gtgatcatgg cctgctttat gtcaggtctg ctggtaggag ctctagtgtg gttcctgaaa 27480 cgcaagccca ggtatggaaa tgaggagaag gaaaaattgc tataaattct ttttcttttc 27540 acagcaccat gaatactttg accagtgtcg tgctgctctc tcttcttgta gcttttagtc 27600 aggcaggaat tattaactta aatgtatcat ggggaatgaa tctaacttta gtgggaccct 27660 cagacctgcc agttacatgg tatgatggaa agggaatgca gttttgtgat ggaaatacaa 27720 ttaagaaccc acaaatcaag catagctgta atcaacagaa tctaacttta cttaatgctg 27780 acaagtctca tgaaagaact tacctaggtt acagacatga cagtaaggga aaagtagact 27840 ataaggttac agtcatacca cctcctccag ccactcgcaa gcctttgtca gagcctcatt 27900 atgttactgt gactatgggc gataacataa ctttagtggg accctcagac ctgccagtta 27960 catggtatga tggagaagga aataaattct gcgatggaga aaaagttgaa catgcagaat 28020 ttaatcatac atgtaacatc cagaacctga cactgctctt tgtcaactta acgcataatg 28080 gagcatacat tggttataac aaagacggtt ctgatagaga attatatgag gtgtcagtca 28140 aaaccttgtt tcagaacggg gctggacaaa gtaaggttga acaaggtaat aaagggaaac 28200 ctaatactgc tcaaagtggt ggtaaaaaaa ccaaaacaga acatagaaac cagagtccaa 28260 aaagaaaatc aacaaataac cttcagccaa cacaattgta tgttaggcct tttactaatg 28320 ttagtttaac tggacctcca aatggcaagg ttacttggta tgatggcgaa cttaatgatc 28380 catgtgaaca aaagtacaaa ctcagaactt tttgcaatca gcaaaatcta actttaatta 28440 atgtaagcag cacttatgat ggcatctatt atggcactga tgaaaaagat aaggcaaatc 28500 gttacagaat aaaagtaaat actacaaatc acaaaactgt taaaattaag ccacatacca 28560 gagaacctcc tgctgtacaa gaaaaacagt ttgaattaca agatgcagaa actgatgaaa 28620 acgaatcaaa aattccctca gctactgtgg caatcgtggt gggagtgatt gcgggctttg 28680 taactctgat cattgtcttc atatgctaca tctgctgccg caagcgtccc aggtcataca 28740 atcatatggt agacccacta ctcagcttct cttactgaaa ctcagtcact ctcatttcag 28800 aaccatgaag gctttcacag cttgcgttct gattagcata gtcacactta gttcagctgc 28860 aatgattaat gttaatgtca ctagaggtgg taaaattaca ttgaatggga cttatccaca 28920 aactacatgg acaagatatc ataaagatgg atggaaaaat atctgtgaat ggaatgttac 28980 tgcatataaa tgcttcaata atggaagcat tactattact gccactgcca acattacttc 29040 tggcacatac aaagctgaaa gctataaaaa tgaaatcaaa aaactaacct ataaaaacaa 29100 caaaaccaca tttgaagatt ctggaaatta tgaacatcaa aaattatctt tttatatgtt 29160 gacaataatt gaactgccta caactaaggc tcccaccaca accagtacaa ctattaagac 29220 cactactcac actacagtgc aaaatactac tttattgatt gggtttttac tgagagagaa 29280 tgaaagtact actgaacaga cagaggctac ctcaagtgcc ttcagcagca ctgcaaattt 29340 aacttcgctt gcttggacta atgaaaccgg agtatcattg atgcatggcc agccttactc 29400 aggtttggat attcaaatta cttttctggt tgtctgtggg atctttattc ttgtggttct 29460 tctgtacttt gtctgctgca aagccagaga gaaatctagg aggcccatct acaggccagt 29520 gattggggaa cctcagccac tccaagtgga tggaggctta aggaatcttc tcttctcttt 29580 tacagtatgg tgatcagcca tgattcctag gttcttccta tttaacatcc tcttttgtct 29640 cttcaacatc tgtgctgcct tcgcggccgt ctcgcacgcc tcgcccgact gtctagggcc 29700 tttccccacc tacctcctct ttgccctgct cacctgcacc tgcgtctgca gcattgtctg 29760 cgtggtcatc accttcctgc agctcatcga ctggtgctgc gcgcgctata attatctcca 29820 ccacagtccc gaatacaggg acgagaacgt agccagaatc ttaaggctca tctgaccatg 29880 cagactctgc tcatgctgct atccctccta tcccctgccc tcgccacttc tgctgactct 29940 aaatgcaaat tcgcggagat atggaatttc ttagattgct atcaggagaa aattgatatg 30000 ccctcctatt acttggtgat tgtgggaata gtcatggtct gctcctgcac tttctttgcc 30060 atcatgatct acccctgttt tgatctcggc tggaactctg ttgaggcatt cacatacaca 30120 ctagaaagca gttcactagc ctccacgcca ccgcccacac cgcctccccg cagaaatcag 30180 ttcccactga ttcagtactt agaagagccc cctccccggc ccccttccac tgttagctac 30240 tttcacataa ccggcggcga tgactgacca ccacctggac ctcgagatgg acggccaggc 30300 ctccgagcag cgcatcctgc aactgcgcgt ccgtcagcag caggagcggg ccgccaagga 30360 gctcctcgat gccatcaaca tccaccagtg caagaagggc atcttctgcc ttgtcaaaca 30420 ggcaaagatc acctacgagc tcgtgtccgg cggcaagcag catcgcctca cctatgagct 30480 gccccagcag aagcagaagt tcacctgcat ggtgggcgtc aaccccatag tcatcaccca 30540 gcagtcgggc gagaccaacg gctgcatcca ctgctcctgc gaaagccccg agtgcatata 30600 ctccctcctc aagacccttt gcggactccg cgacctcctc cccatgaacg gaagcggagc 30660 tactaacttc agcctgctga agcaggctgg tgacgtcgag gagaatcctg gcccaatggt 30720 gagcaaaggc gaagagctgt tcaccggcgt ggtgcccatc ctggtggagc tggacggcga 30780 cgtgaacggc cacaagttca gcgtgagcgg cgagggcgag ggcgacgcca cctacggcaa 30840 gctgaccctg aagctgctgt gcaccaccgg caagctgccc gtgccctggc ccaccctggt 30900 gaccaccctg ggctacggcg tgcagtgctt cgcccggtac cccgaccaca tgaagcagca 30960 cgacttcttc aagagcgcca tgcccgaggg ctacgtgcag gagcggacca tcttcttcaa 31020 ggacgacggc aactacaaga cccgggccga ggtgaagttc gagggcgaca ccctggtgaa 31080 ccggatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg gccacaagct 31140 ggagtacaac tacaacagcc acaacgtgta catcaccgcc gacaagcaga agaacggcat 31200 caaggccaac ttcaagatcc ggcacaacat cgaggacggc ggcgtgcagc tggccgacca 31260 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 31320 gagctaccag agcgccctgt tcaaggaccc caacgagaag cgggaccaca tggtgctgct 31380 ggagttcctg accgccgccg gcatcaccga gggcatgaac gagctctata agtgatgttg 31440 attaaaagca cgaaaaccaa tcagaccctt cccccatttc cccattccca attactcata 31500 aaataaatca ttggaattaa tcattcaata aaaatcactt acttgaaatc tgaaagtatg 31560 tctctggtgt agttgttcag cagcacctcg ttaccctcct cccagctctg gtattccagt 31620 ccccggcggg cggcgaactt cctccacacc ttgaaaggga tgtcaaattc ctggtccaca 31680 attttcattg tcttccctct cagatgtcaa agaggctccg ggtggaagat gacttcaacc 31740 ccgtctaccc ctatggctac gcgcggaatc agaatatccc cttcctcact cccccctttg 31800 tctcctccga tggattccaa aacttccccc ctggggtcct gtcactcaaa ctggctgacc 31860 caatagccat cgtcaatggg aatgtctcac tcaaagtggg agggggtctc actttgcaag 31920 atggaactgg aaaactaaca gtcaatgctg atccaccttt gcaacttaca aacaacaaat 31980 tagggattgc tttggacgct ccatttgatg ttatagacaa taaactcaca ttgttagcgg 32040 gccatggctt gtctattata acaaaagaaa catcaacact gcctggcttg attaatactc 32100 ttgtagtatt aactggaaag ggtattggaa cagaatcaac agataatggc ggaagcgtat 32160 gtgttagagt tggagaaggt ggcggcttat catttaataa tgatggagac ttggtagcat 32220 ttaataaaaa agaagataag cgcaccctat ggacaactcc agacacatct ccaaattgca 32280 agattgatca agataaggac tctaagttaa ctctggtcct tacaaagtgt ggaagtcaaa 32340 tattggctaa tgtgtcatta attgtcgtag ctggtaagta caaaattatc aataacaata 32400 ctcaaccagc tctcaaagga tttaccatta aattattgtt tgatgaaaat ggagtactta 32460 tggaatcttc aaatcttggt aaatcatatt ggaactttag aaatgaaaat tcaattatgt 32520 caacagctta tgaaaaagct attggattca tgcctaattt ggtagcctat ccaaaaccta 32580 ccgctggctc taaaaaatat gcaagagata tagtttatgg aaacatctac cttggtggaa 32640 agccagatca accagtaacc attaaaacta cctttaatca ggaaactgga tgtgaatatt 32700 ctatcacatt tgattttagt tgggccaaga cttatgtaaa tgttgaattt gaaacaacct 32760 cttttacctt ttcctatatc gcccaagaat gaaagaccaa taaacgtgtt tttcatttca 32820 aaattttaat catgtatctt tattgatttt tacaccagca cgggtagtca gtctcccacc 32880 accagcccat ttcacagtgt acactgttct ctcagcacgg gtggccttaa atagggaaat 32940 gttctcatta gtgcgggaac tggacttggg gtctataatc cacacagttt cctggcgagc 33000 caaacggggg tcggtgattg agatgaagcc gtcctctgaa aagtcatcca agcgagcctc 33060 acagtccaag gtcacagtct ggtggaatga gaagaacgca cagattcata ctcggaaaac 33120 aggatgggtc tgtgcctctc catcagcgcc ctcagcagtc tctgccgccg gggctcggtg 33180 cggctgctgc agattggatc gggatcgcaa gtctctctga ctataatccc cacagccttc 33240 agcatcagtc tcctggtgcg tcgggcacag caccgcatcc tgatctctgc catgttctca 33300 cagtaagtgc agcacataat caccatgtta ttcagcagcc cataattcag ggcgctccag 33360 ccaaagctca tgttggggat gatggaaccc acgtgaccat cgtaccagat gcggcagtat 33420 atcaggtgcc tgcccctcat aaacacactg cccatataca tgatctcttt gggcatgttt 33480 ctgttcacaa tctgacggta ccaggggaag cgctggttga acatgcaccc gtaaatgact 33540 ctcctgaacc acacggccag cagggtgcct cccgcccgac actgcaggga gcccggggat 33600 gaacagtggc aatgcaggat ccagcgctcg tacccgctca ccatctgagc tctcaccaag 33660 tccagggtag cagggcacag gcacactgac atacatcttt ttaaaatttt tatttcctct 33720 ggggtcagga tcatatccca ggggactgga aactcttgga gcagggtaaa gccagcagca 33780 catggtaatc cacggacaga acttacatta tgataatctg catgatcaca atcgggcaac 33840 aggggatgtt gttcagtcag tgaagccctg gtttcctcat cagatcgtgg taaacgggcc 33900 ctgcgatatg gatgatggcg gagcgagctg gattgaatct cggtttgcat tgtagtggat 33960 tctcttgcgt accttgtcgt acttctgcca gcagaaatgg gcccttgaac agcagatacc 34020 cctcctgcgg ccgtcctttc gctgctgccg ctcagtcatc caactgaagt acatccattc 34080 tcgaagattc tggagaagtt cctctgcatc tgatgaaata aaaaacccgt ccatgcgaat 34140 tcccctcatc acatcagcca ggactctgta ggccatcccc atccagttaa tgctgccttg 34200 tctatcattc agagggggcg gtggcaggat tggaagaacc atttttattc caaacggtct 34260 cgaaggacga taaagtgcaa gtcacgcagg tgacagcgtt cccctccgct gtgctggtgg 34320 aaacagacag ccaggtcaaa acccactcta ttttcaaggt gctcgaccgt ggcttcgagc 34380 agtggctcta cgcgtacatc cagcataaga atcacattaa aggctggccc tccatcgatt 34440 tcatcaatca tcaggttaca ttcctgcacc atccccaggt aattctcatt tttccagcct 34500 tgaattatct ctacaaattg ttggtgtaag tccactccgc acatgtggaa aagctcccac 34560 agtgccccct ccactttcat aatcaggcag accttcataa tagaaacaga tcctgctgct 34620 ccaccacctg cagcgtgttc aaaacaacaa gattcaataa ggttctgccc tccgccctga 34680 gctcgcgcct caatgtcagc tgcaaaaagt cacttaagtc ctgggccact acagctgaca 34740 attcagagcc agggctaagc gtgggactgg caagcgtaag ggaaaacttt aatgctccaa 34800 agctagcacc caaaaactgc atgctggaat aagctctctt tgtgtctccg gtgatgcctt 34860 ccaaaatgtg agtgataaag cgtggtagtt tttctttaat catttgcgta atagaaaagt 34920 cctctaaata agtcactagg accccaggga ccacaatgtg gtagcttaca ccgcgtcgct 34980 gaagcatggt tagtagagat gagagtctga aaaacagaaa gcatgcacta aactaaggtg 35040 gctattttta ctgaaggaaa aatcactctc tccagcaaca gggtacccac tgggtggccc 35100 ttgcggacat acaaaaatcg gtccgtgtga ttaaaaagca gcacagtaag ttcctgtctt 35160 cttccggcaa aaatcacatc ggactgggtt agtatgtccc tggcatggta gtcattcaag 35220 gccataaatc tgccctgata tccagtagga accagcacac tcacttttag gtgaagcaat 35280 accaccccat gcggaggaat gtggaaagat tcagggcaaa agaaggtata tctattgcta 35340 gtcccttcct ggacgggagc gatccctcca ggactatcta tgaaagcata cagagattca 35400 gccatagctc agcccgctta ccagtagaca gagagcacag cagtacaagc gccaacagca 35460 gcgactgact acccactaac ccagctccct atttaaaggc accttacact gacgtaatga 35520 ccaaaggtct aaaaaccccg ccaaaaaaac acacacgccc tgggtgtttt ttcgcgaaaa 35580 cacttccgcg ttctcacttc ctcgtatcga tttcgtgact caacttccgg gttcccacgt 35640 tacgtcactt ctgcccttac atgtaactca gtcgtagggc gccatgttgc ccacgtccaa 35700 aatggtttcc atgtccagcc acgcctccgc ggcgaccgtt agccgtgcgt cgtgacgtca 35760 tttgcatcgt cttctctcgt ccaatcagcg ttggctccgc cccgaaaccg ttaaaattca 35820 aaagctcatt tgcatattaa cttttgttta ctttgtgggg tatattatat agatag 35876 <210> 23 <211> 35563 <212> DNA <213> Artificial Sequence <220> <223> Synthetic adenovirus genome Ad34 E3-14.8k-P2A-YPet <400> 23 catcatcaat aatatacctt atagatggaa tggtgccaat atgtaaatga ggtgatttta 60 aaaattgtgg ggtgtgtggt gattggctgt ggggttaacg gctaaacggg gcggcgcggc 120 cgtgggaaaa tgacgttttg tgggggtgga gtttttttgc aagttgtcgc gggaaatgtg 180 acgcataaaa aggctttttt tctcacggaa ctactgactt ttcccacggt atttaacagg 240 aaatgaggta gttttgaccg gatgcaagtg aaaattgctg atttgcgcgc gaaaactgaa 300 tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360 ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420 ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctacggtatt 480 tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540 tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600 aatttctgct gagactggaa atgaaatact ggagcttgtg gtgcacgccc tgatgggaga 660 cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720 agaggtagag ggatcggagg attctaatga ggaagctgtg aatggctttt ttaccgattc 780 tatgctttta gctgctaatg aaggattaga attagatccg cctttggaca ctttcgatac 840 tccaggggtg attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgggttccgt 900 ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960 aaaggagcag tctatgcaga ctgcagcggg tgagggagtg aaggctgcca gtgttggttt 1020 tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080 aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagagcgc actgccactt 1140 tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200 attgagtggg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgagtcacc 1260 atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320 caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380 cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacggc caagacaata 1440 agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaga gtgcaatgta 1500 ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560 taagtagaag cagacctgta tggttagctc ataggagctg gctttcatcc atggaggttt 1620 gggccatttt ggaagacctt agaaagacta ggcaactgtt agaggacgct tcggacggag 1680 tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740 aacaggacta taaagaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800 ctcttaattt gggccatcaa gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860 caaccccagg tagaactgcc gctgctgtgg cttttcttac ttttatatta gataaatgga 1920 tcccgcagac tcatttcagc aggggatacg ttttggattt cgtagccaca gcattgtgga 1980 gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040 gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100 aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160 gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220 taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280 gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtc cagaaagagg gaagggatga 2340 agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400 tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460 acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520 ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580 gtggcctgga gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640 tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700 ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760 tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820 atgcatattc caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880 ctgcgcttct acagatactg gatgttttat tttaattaag ggcaatgcca gcgtaaagca 2940 taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgccggtgg 3000 gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060 ttttgatcac aatgtgttga ccaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120 tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180 cagaatgagc ctaacaggaa tctttgacat gaacatgcaa atctggaaga tcctgaggta 3240 tgatgatacg agatcgaggg tgcgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300 gccggtgtgt gtagatgtga ctgaagatct gagaccggat catttggtta ttgcccgcac 3360 tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420 ggggtggggt tttcagatgg acagattgag taaaaatttg ttttttctgt ctttcagctg 3480 tcatgagtgg aaacgcttct tttaaggggg gagtcttcag cccttatctg acagggcgtc 3540 tcccatcctg ggcaggagtt cgtcagaatg ttatgggatc tactgtggat ggaagacccg 3600 tccaacccgc caattcttca acgctgacct atgctacttt aagttcttca cctttggacg 3660 cagctgcagc cgccgccgcc gcctctgttg ccgctaacac tgtgcttgga atgggttact 3720 atggaagtat cgtggctaat tccacttcct ctaataaccc ttctaccctg actcaggaca 3780 agttacttgt ccttttggcc cagctggagg ctttgaccca acgtctgggt gaactttatc 3840 agcaggtggc cgagttgcga gtacaaactg agtctgctgt cggcacggca aagtctaaat 3900 aaaaaaaaat tccacaatca atgaataaat aaacgagctt gttgttgatt taaaatcaag 3960 tgtttttatt tcatttttcg cgcacggtat gccctagacc accgatctcg atcattgaga 4020 acacggtgga ttttttccag aatcctatag aggtgggatt gaatgtttag atacatgggc 4080 attaggccat ctttggggtg gagatagctc cattgaaggg attcatgctc cggggtagtg 4140 ttgtaaatca cccagtcata acaaggtcgc agtgcatggt gttgcacaat atcttttaga 4200 agtaggctga ttgccacaga taagcccttg gtgtaggtgt ttacaaaccg gttgagctgg 4260 gaggggtgca ttcggggtga aattatgtgc attttggatt ggatttttaa gttggcaata 4320 ttgccgccaa gatctcgtct tgggttcatg ttatgaagga ccaccaagac ggtgtatccg 4380 gtacatttag gaaatttatc gtgtagcttg gatggaaaag cgtggaaaaa tttggagaca 4440 cccttgtgtc ctccgagatt ttccatgcac tcatccatga taatagcaat ggggccgtgg 4500 gcagcagcgc gggcaaacac gttccgtggg tctgacacat catagttatg ttcctgagtt 4560 aaatcatcat aagccatttt aatgaatttg gggcggagag tacccgattg gggtatgaat 4620 gttccttcgg gccccggagc atagttcccc tcacagattt gcatttccca agctttcagt 4680 tccgatggtg gaatcatgtc cacctggggg gctatgaaga acaccgtttc tggggcgggg 4740 gtgattagtt gggatgatag caagtttctg agcaattgag atttgccaca tccggtgggg 4800 ccataaatga ttccgattac aggttgcagg tggtagttta gggaacggca actgccgtct 4860 tctcgaagca agggggccac ctcgttcatc atttccctta catgcatatt ttcccgcacc 4920 aaatccatta ggaggcgctc tcctcctagt gatagaagtt cttgtagtga ggaaaagttt 4980 ttcagcggtt ttagaccgtc agccatgggc attttggaga gagtttgctg caaaagttct 5040 agtctgttcc acagttcagt gatgtgttct atggcatctc gatccagcag acctcctcgt 5100 ttcgcgggtt tggacggctc ctggagtagg gtatgagacg atgggcgtcc agcgctgcca 5160 gggttcggtc cttccagggt ctcagtgttc gagtcagggt tgtttccgtc acagtgaagg 5220 ggtgtgcgcc tgcttgggcg cttgccaggg tgcgcttcag actcattctg ctggtggaga 5280 acttctgtcg cttggcgccc tgtatgtcgg ccaagtagca gtttaccatg agttcgtagt 5340 tgagcgcctc ggctgcgtgg cctttggcgc ggagcttacc tttggaagtt ttcttgcata 5400 ccgggcagta taggcatttc agcgcataca gcttgggcgc aaggaaaatg gattctgggg 5460 agtatgcatc tgcgccgcag gaggcgcaaa cagtttcaca ttccaccagc caggttaaat 5520 ccggttcatt ggggtcaaaa acaagttttc cgccatattt tttgatgcgt ttcttacctt 5580 tggtctccat gagttcgtgt cctcgttgag tgacaaacag gctgtccgta tccccgtaga 5640 ctgattttac aggcctcttc tccagtggag tgcctcggtc ttcttcgtac aggaactctg 5700 accactctga tacaaaggcg cgcgtccagg ccagcacaaa ggaggctatg tgggaggggt 5760 agcgatcgtt gtcaaccagg gggtccacct tttccaaagt atgcaaacac atgtcaccct 5820 cttcaacatc caggaatgtg attggcttgt aggtgtattt cacgtgacct ggggtccccg 5880 ctgggggggt ataaaagggg gcggttcttt gctcttcctc actgtcttcc ggatcgctgt 5940 ccaggaacgt cagctgttgg ggtaggtatt ccctctcgaa ggcgggcatg acctctgcac 6000 tcaggttgtc agtttctaag aacgaggagg atttgatatt gacagtgccg gttgagatgc 6060 ctttcatgag gttttcgtcc atttggtcag aaaacacaat ttttttattg tcaagtttgg 6120 tggcaaatga tccatacagg gcgttggata aaagtttggc aatggatcgc atggtttggt 6180 tcttttcctt gtccgcgcgc tctttggcag cgatgttgag ttggacatac tcgcgtgcta 6240 ggcacttcca ttcggggaag atagttgtca attcatctgg cacgattctc acttgccacc 6300 ctcgattatg caaggtaatt aaatccacac tggtggccac ctcgcctcga aggggttcgt 6360 tggtccaaca gagcctacct cctttcctag aacagaaagg gggaagtggg tctagcataa 6420 gttcatcggg agggtctgca tccatggtaa agattcccgg aagtaaatcc ttatcaaaat 6480 agctgatggg agtggggtca tctaaggcca tttgccattc tcgagctgcc agtgcacgct 6540 catatgggtt aaggggactg ccccagggca tgggatgggt gagtgcagag gcatacatgc 6600 cacagatgtc atagacgtag atgggatcct caaagatgcc tatataggtt ggatagcatc 6660 gcccccctct gatacttgct cgcacatagt catatagttc atgtgatggc gctagcaacc 6720 ccggacccaa gttggtgcga ttgggttttt ctgttctgta gacaatctgg cgaaagatgg 6780 cgtgagaatt ggaagagatg gtgggtcttt gaaaaatgtt gaaatgggca tgaggtagac 6840 ctacagagtc tctgacaaag tgggcataag attcttgaag cttggttacc agttcggcgg 6900 tgacaagtac gtctagggcg cagtagtcaa gtgtttcttg aatgatgtca taacctggtt 6960 ggtttttctt ttcccacagt tcgcggttga gaaggtattc ttcgcgatcc ttccagtact 7020 cttctagcgg aaacccgtct ttgtctgcac ggtaagatcc tagcatgtag aactgattaa 7080 ctgccttgta agggcagcag cccttctcta cgggtagaga gtatgcttga gcagcttttc 7140 gcagcgaagc gtgagtaagg gcgaaggtgt ctctgaccat gactttgaga aattggtatt 7200 tgaagtccat gtcgtcacag gctccctgtt cccagagttg gaagtctacc cgtttcttgt 7260 aggcggggtt gggcaaagcg aaagtaacat cgttgaagag aatcttaccg gctctgggca 7320 taaaattgcg agtgatgcgg aaaggctgtg gtacttccgc tcgattgttg atcacctggg 7380 cagctaggac gatctcgtcg aaaccgttga tgttgtgtcc tacgatgtat aattctatga 7440 aacgcggcgt gcctttgacg tgaggtagct tattgagctc atcaaaggtt aggtctgtag 7500 ggtcagataa ggcgtagtgt tcgagagccc attcgtgcag gtgaggattt gcatgtagga 7560 atgatgacca aagatccacc gccagtgctg tttgtaactg gtcccgatac tgacgaaaat 7620 gctggccaat tgccattttt tctggagtga cacagtagaa ggttctgggg tcttgttgcc 7680 atcgatccca ctttagttta atggctagat cgtgggccat gttgacgaga cgctcttctc 7740 ctgagagttt catgaccagc atgaaaggaa ctagttgttt gccaaaggac cccatccagg 7800 tgtaagtttc cacatcgtag gtcaggaaga gtctttctgt gcgaggatga gagccgatcg 7860 ggaagaactg gatttcctgc caccagttgg aggattggct gttgatgtga tggaagtaga 7920 agtttctgcg gcgcgccgag cattcgtgtt tgtgcttgta cagacggccg cagtagtcgc 7980 agcgttgcac gggttgtatc tcgtgaatga gctgtacctg gcttcccttg acgagaaatt 8040 tcagtgggaa gccgaggcct ggcgattgta tctcgtgctc ttctatattc gctgtatcgg 8100 cctgttcatc ttctgtttcg gtggtggtca tgctgacgag cccccgcggg aggcaagtcc 8160 agacctcggc gcgggagggg cggagctgaa ggaccagagc gcgcaggctg gagctgtcca 8220 gagtcctgag acgctgcgga ctcaggttag taggtaggga cagaagatta acttgcatga 8280 tcttttccag ggcgtgcggg aggttcagat ggtacttgat ttccacaggt tcgtttgtag 8340 agatgtcaat ggcttgcagg gttccgtgtc ctttgggcgc cactaccgta cctttgtttt 8400 ttcttttgat cggtggtggc tctcttgctt cttgcatgct cagaagcgat gacggggacg 8460 cgcgccgggc ggaagcggtt gttccggacc cggaggcatg gctggtagtg gcacgtcggc 8520 gccgcgcacg ggcaggttct ggtactgcgc tctgagaaga cttgcgtgcg ccaccacgcg 8580 tcgattgacg tcttgtatct gacgtctctg ggtgaaagct accggccccg tgagcttgaa 8640 cctgaaagag agttcaacag aatcaatttc ggtatcgtta acggcagctt gtctcagtat 8700 ttcttgtacg tcaccagagt tgtcctggta ggcgatctcc gccatgaact gctcgatttc 8760 ttcctcctga agatctccgc gacccgctct ctcgacggtg gccgcgaggt cattggagat 8820 acggcccatg agttgggaga atgcagtcat gcccgcctcg ttccagacgc ggctgtaaac 8880 cacggccccc tcggagtctc ttgcgcgcat caccacctga gcgaggttaa gctccacgtg 8940 tctggtgaag accgcatagt tgcataggcg ctgaaaaagg tagttgagtg tggtggcaat 9000 gtgttcggcg acgaagaaat acatgatcca tcgtctcagc ggcatttcgc tgacatcgcc 9060 cagagcttcc aagcgctcca tggcctcgta gaagtccacg gcaaaattaa aaaactggga 9120 gtttcgcgcg gacacggtca attcctcctc gagaagacgg atgagttcgg ctatggtggc 9180 ccgtacttcg cgttcgaagg ctcccgggat ctcttcttcc tcttctatct cttcttccac 9240 taacatctct tcttcgtctt caggcggggg cggagggggc acacggcgac gtcgacggcg 9300 cacgggcaaa cggtcgatga atcgttcaat gacctctccg cggcggcggc gcatggtttc 9360 agtgacggcg cggccgttct cgcgcggtcg cagagtaaaa acaccgccgc gcatctcctt 9420 aaagtggtga ctgggaggtt ctccgtttgg gagggagagg gcgctgatta tacattttat 9480 taattggccc gtagggactg cgcgcagaga tctgatcgtg tcaagatcca cgggatctga 9540 aaacctttcg acgaaagcgt ctaaccagtc acagtcacaa ggtaggctga gtacggcttc 9600 ttgtgggcgg gggtggttat gtgttcggtc tgggtcttct gtttcttctt catctcggga 9660 aggtgagacg atgctgctgg tgatgaaatt aaagtaggca gttctaagac ggcggatggt 9720 ggcgaggagc accaggtctt tgggtccggc ttgctggata cgcaggcgat tggccattcc 9780 ccaagcatta tcctgacatc tagcaagatc tttgtagtag tcttgcatga gccgttctac 9840 gggcacttct tcctcacccg ttctgccatg catacgtgtg agtccaaacc cgcgcattgg 9900 ttgtaccagt gccaagtcag ctacgactct ttcggcgagg atggcttgct gtacttgggt 9960 gagggtggct tgaaagtcat caaaatccac aaagcggtgg taagccccgg tattaatggt 10020 gtaagcacag ttggccatga ctgaccagtt aactgtctgg tgaccagggc gcacgagctc 10080 ggtgtattta aggcgcgaat aggcgcgggt gtcaaagatg taatcgttgc aggtgcgcac 10140 cagatactgg taacctataa gaaaatgcgg cggtggttgg cggtagagag gccatcgttc 10200 tgtagctgga gcgccggggg cgaggtcttc caacataagg cggtgatagc cgtagatgta 10260 cctggacatc caggtgattc ctgcggcggt agtagaagcc cgaggaaact cgcgtacgcg 10320 gttccaaatg ttgcgtagcg gcatgaagta gttcattgta ggcacggttt gaccagtgag 10380 gcgcgcgcag tcattgatgc tctatagaca cggagaaaat gaaagcgttc agcgactcga 10440 ctccgtagcc tggaggaacg tgaacgggtt gggtcgcggt gtaccccggt tcgagacttg 10500 tactcgagcc ggccggagcc gcggctaacg tggtattggc actcccgtct cgacccagcc 10560 tacaaaaatc caggatacgg aatcgagtcg ttttgctggt tgccgaatgg cagggaagtg 10620 agtcctattt tttttttttg ccgctcagat gcatcccgtg ctgcgacaga tgcgtcccca 10680 acaacagccc ccctcgcagc agcagcaacc acaaaaggct gtccctgcaa ctactgcaac 10740 tgccgctgtg agcggtgcgg gacagcccgc ctatgatctg gacttggaag agggcgaagg 10800 actggcacgt ctaggtgcgc cttcgcccga gcggcatccg cgagttcaac tgaaaaaaga 10860 ttctcgcgag gcgtatgtgc cccaacagaa cctatttaga gacagaagcg gcgaggagcc 10920 ggaggagatg cgagcttccc gctttaacgc gggtcgtgag ctgcgtcacg gtttggacag 10980 aagacgagtg ttgcgggacg aggatttcga agttgatgaa gtgacaggga tcagtcctgc 11040 cagggcacac gtggctgcag ccaaccttgt atcggcttac gaacagacag taaaggaaga 11100 gcgtaatttc caaaagtctt ttaataatca tgtgcgaacc ctcattgccc gcgaagaagt 11160 cacccttggt ttgatgcatt tgtgggattt gatggaagct atcattcaga accctactag 11220 caaacctctg accgcacagc tgtttctggt ggtgcaacac agcagagaca atgaggcttt 11280 cagagaggcg ctgctcaaca tcaccgaacc cgaggggaga tggttgtatg atcttatcaa 11340 cattctacag agtatcatag tgcaggagcg gagcctgggc ctggccgaga aggtggctgc 11400 catcaattac tcggttttga gcttgggaaa gtattacgct cgcaagatct acaagactcc 11460 atacgttccc atagacaagg aggtgaagat agatgggttc tacatgcgca tgacgctgaa 11520 ggtgttgacc ctgagcgatg atcttggggt gtaccgcaat gacagaatgc atcgcgcggt 11580 gagcgccagc aggaggcgcg agttaagcga cagggaactg atgcacagtt tgcaaagagc 11640 tctaactgga gctggaaccg agggtgagaa ttactttgat atgggagctg acttgcagtg 11700 gcagcctagt cgcagggctc tgaacgccgc gacggcagga tgtgagcttc cttacataga 11760 agaggcggat gaaggcgagg aggaagaggg cgagtacttg gaagactgat ggcacaaccc 11820 gtgttttttg ctagatggaa cagcaagcac cggatcccgc aatgcgggcg gcgctgcaga 11880 gccagccgtc cggcattaac tcctcggacg attggaccca ggccatgcaa cgtatcatgg 11940 cgttgacgac tcgcaacccc gaagccttta gacagcaacc ccaggccaac cgtctatcgg 12000 ccatcatgga agctgtagtg ccttcccgct ctaatcccac tcatgagaag gtcctggcca 12060 tcgtgaacgc gttggtggag aacaaagcta ttcgtccaga tgaggccgga ctggtataca 12120 acgctctctt agaacgcgtg gctcgctaca acagtagcaa tgtgcaaacc aatttggacc 12180 gtatgataac agatgtacgc gaagccgtgt ctcagcgcga aaggttccag cgcgatgcca 12240 acctgggttc gctggtggcg ttaaatgctt tcttgagtac tcagcctgct aatgtgccgc 12300 gtggtcaaca ggattatact aactttttaa gtgctttgag actgatggta tcagaagtac 12360 ctcagagcga agtatatcag tccggtcctg attacttctt tcagactagc agacagggct 12420 tgcagacggt aaatctgagc caagctttta aaaaccttaa aggtttgtgg ggagtgcatg 12480 ccccggtagg agaaagagca accgtgtcta gcttgttaac tccgaactcc cgcctattat 12540 tactgttggt agctcctttc accgacagcg gtagcatcga ccgtaattcc tatttgggtt 12600 acctactaaa cctgtatcgc gaagccatag ggcaaagtca ggtggacgag cagacctatc 12660 aagaaattac ccaagtcagt cgcgctttgg gacaggaaga cactggcagt ttggaagcca 12720 ctctgaactt cttgcttacc aatcggtctc aaaagatccc tcctcaatat gctcttactg 12780 cggaggagga gaggatcctt agatatgtgc agcagagcgt gggattgttt ctgatgcaag 12840 agggggcaac tccgactgca gcactggaca tgacagcgcg aaatatggag cccagcatgt 12900 atgccagtaa ccgacctttc attaacaaac tgctggacta cttgcacaga gctgccgcta 12960 tgaactctga ttatttcacc aatgccatct taaacccgca ctggctgccc ccacctggtt 13020 tctacacggg cgaatatgac atgcccgacc ctaatgacgg atttctgtgg gacgacgtgg 13080 acagcgatgt tttttcacct ctttctgatc atcgcacgtg gaaaaaggaa ggcggcgata 13140 gaatgcattc ttctgcatcg ctgtccgggg tcattggtgc taccgcggct gagcccgagt 13200 ctgcaagtcc ttttcctagt ctaccctttt ctctacacag tgtacgtagc agcgaagtgg 13260 gtagaataag tcgcccgagt ttaatgggcg aagaggagta cctaaacgat tccttgctca 13320 gaccggcaag agaaaaaaat ttcccaaaca atggaataga aagtttggtg gataaaatga 13380 gtagatggaa gacttatgct caggatcaca gagacgagcc tgggatcatg gggactacaa 13440 gtagagcgag ccgtagacgc cagcgccatg acagacagag gggtcttgtg tgggacgatg 13500 aggattcggc cgatgatagc agcgtattgg acttgggtgg gagaggaagg ggcaacccgt 13560 ttgctcattt gcgccctcgc ttgggtggta tgttgtaaaa aaaaataaaa aagaaaaaac 13620 tcaccaaggc catggcgacg agcgtacgtt cgttcttctt tattatctgt gtctagtata 13680 atgaggcgag tcgtgctagg cggagcggtg gtgtatccgg agggtcctcc tccttcgtac 13740 gagagcgtga tgcagcagca gcaggcgacg gcggtgatgc aatccccact ggaggctccc 13800 tttgtgcctc cgcgatacct ggcacctacg gagggcagaa acagcattcg ttactcggaa 13860 ctggcacctc agtacgatac caccaggttg tatctggtgg acaacaagtc ggcggacatt 13920 gcttctctga actatcagaa tgaccacagc aacttcttga ccacggtggt gcaaaacaat 13980 gactttaccc ctacggaagc cagcacccag accattaact ttgatgaacg atcgcggtgg 14040 ggcggtcagc taaaaaccat catgcatact aacatgccca acgtgaacga gtatatgttt 14100 agtaacaagt tcaaagcgcg tgtgatggtg tccagaaaac ctcctgaggg tgttagagta 14160 gacgataatt atgatcataa gcaagatatt ctaaaatacg agtggttcga gtttactttg 14220 ccagaaggca acttttcggt cactatgact atcgacttga tgaacaatgc catcatagac 14280 aattacttga aagtgggcag acagaatgga gtgttggaaa gtgacattgg tgttaagttc 14340 gacactagga acttcaagtt gggatgggat ccagaaacta agttgatcat gcctggggtt 14400 tacacctatg aggccttcca tcctgacatc gtattgctgc ctggctgcgg agtggacttt 14460 accgaaagcc gtctgagcaa ccttcttggc attagaaaga aacacccatt ccaagagggt 14520 tttaagatct tgtatgagga tttagaagga ggaaatattc cagccctttt ggatgtagat 14580 gcttatgaga acagcaagaa agatcaaaaa gccaaaatag aagctgctgc agaagctaaa 14640 gcaaacatag ttgccaacga tccggtaagg gtggctaacg ctagtgaaat caggggagac 14700 agttttgccg caacatccgt tccgactaaa gaatcattat tggatgatgt gtctcaaaac 14760 atagagttaa aactcactat taagcctgtg gaaaaagatg gcaaaaacag aagttacaat 14820 gtgttggaag ataaaatcaa cacggcctat cgcagttggt acctttcgta caattatggc 14880 gaccccgaaa aaggagtgcg ttcctggaca ttgctcacca cctcagatgt cacctgcgga 14940 gcggagcagg tctactggtc gcttccagac atgatgcagg atcctgtcac tttccgctcc 15000 actagacaag tcagtaacta ccctgtggtg ggtgcagagc ttatgcccgt cttttcaaag 15060 agcttctaca acgaacaagc tgtgtactcc cagcagctcc gccagtccac ctcgcttacg 15120 cacgtcttca accgctttcc tgagaaccag attttaatcc gtccgccggc gcccacaatt 15180 accaccgtca gtgaaaacgt tcctgctctc acagatcacg ggaccctgcc gttgcgcagc 15240 agtatccggg gagtccaacg tgtgaccgtt actgacgcca gacgccgcac ctgtccctac 15300 gtgtacaagg cactgggcat agtcgcaccg cgcgtccttt caagccgcac tttctaaaaa 15360 aaaaaaaaaa atgtccgttc ttatctcgcc cagtaataac accggttggg gtctgcgcgc 15420 tcccagcaag atgtacggag gcgcacgcaa acgttctacc caacatcccg tgcgtgttcg 15480 cgggcatttt cgcgctccat ggggtgccct caagggccgc actcgcgttc gaaccaccgt 15540 cgatgatgta atcgatcagg tggttgccga cgcccgtaat tatactccta ctgcgcctac 15600 atctactgtg gacgcagtta ttgacagtgt agtggctgac gctcgcaact atgctcgacg 15660 taagagccgg cgaaggcgca ttgccagacg tcaccgagct accactgcca tgcgagcagc 15720 aagagctctg ctacgaagag ctagacgcgt ggggcgaaga gccatgctta gggcggccag 15780 acgtgcagct tcgggcgcca gcgccggcag gtcccgcagg caagcagccg ctgtcgcagc 15840 ggcgactatt gccgacatgg cccaatcgcg aagaggcaat gtatactggg tgcgtgacgc 15900 tgccaccggt caacgtgtac ccgtgcgcac ccgtccccct cgcacttaga agatactgag 15960 cagtctccga tgttgtgtcc cagcggcgag gatgtccaag cgcaaataca aggaagaaat 16020 gctgcaggtt atcgcacctg aagtctacgg ccaaccgttg aaggatgaaa aaaaaccccg 16080 caaaatcaag cgggtaaaaa aggacaaaaa agaagaggaa gatggcgatg atgggctggc 16140 ggagtttgtg cgcgagtttg ccccacggcg acgcgtgcaa tggcgtgggc gcaaagttcg 16200 acatgtgttg agacctggaa cttcggtggt ctttacaccc ggcgagcgtt caagcgctac 16260 ttttaagcgt tcctatgatg aggtgtacgg ggatgatgat attcttgagc aggcagctga 16320 ccgattaggc gagtttgctt atggcaagcg tagtagaata aatcccaagg atgaaacagt 16380 gtccataccc ttggatcatg gaaatcccac ccctagtctt aaaccggtca ctttgcagca 16440 agtgttaccc gtaactccgc gaacaggtgt taaacgcgaa ggtgaagatt tgtatcccac 16500 tatgcaactg atggtgccca aacgccagaa gttggaggac gttttggaga aagtaaaagt 16560 ggatccagat attcaacctg aggttaaagt gagacccatt aagcaggtag cgcctggtct 16620 gggagtacaa actgtagaca ttaaaattcc cactgaaagt atggaagtgc aaactgaacc 16680 cgcaaagcct actgccacct ccactgaagt gcaaacggac ccatggatgc ccatgcctat 16740 tacaactgac gccgtcggtc ccactcgaag atcccgacga aagtacggtc cagcaagtct 16800 gttgatgccc aactatgtcg tacacccatc tattattcct actcctggtt accgaggcac 16860 tcgctactat cgcagccgaa acagtacttc ccgccgtcgc cgcaagacac ctgcaaatcg 16920 cagtcgtcgc cgtagacgca caagcaaacc gattcccggc gccctggtgc ggcaagtgta 16980 ccgcaatggt agtgcggaac ctttgacact gccgcgtgcg cgttaccatc ctagtatcat 17040 cacttaatca atgttgccgc tgcctccttg cagatatggc cctcacttgt cgccttcgcg 17100 ttcccatcac tggttaccga ggaagaaact cgcgccgtag aagagggatg ttggggcgcg 17160 gaatgcgacg ctacaggcga cggcgtgcta tccgcaagca attgcggggt ggttttttgc 17220 cagccttaat tccaattatc gctgctgcga ttggcgcaat accaggcata gcttccgtgg 17280 cggttcaggc ctcgcaacga cattgacatt ggaaaaaaaa aaaacgtata aataaaaaat 17340 acaatggact ctgacactcc tggtactgtg actatgtttt cttagagatg gaagacatca 17400 atttttcatc cttggctccg cgacacggca cgaagccgta catgggcacc tggagcgaca 17460 tcggcacgag ccaactgaac gggggcgcct tcaattggag cagtatctgg agcgggctta 17520 aaaattttgg ctcaaccata aaaacatacg ggaacaaagc ttggaacagc agtacaggac 17580 aggcgcttag aaataaactt aaagaccaga acttccaaca aaaagtagtc gatgggatag 17640 cttccggtat caatggagtg gtagatttgg ctaaccaggc tgtgcagaaa aagataaaca 17700 gtcgtttgga cccgccgcca gcaaccccag gtgaaatgca agtggaggaa gaaattcctc 17760 cgccagaaaa acgaggcgac aagcgtccgc gtcccgattt ggaagagacg ctggtgacgc 17820 gcgtagatga accgccttct tatgaggaag caacgaagct tggaatgccc accactagac 17880 cgatagcccc tatggccacc ggggtgatga aaccttctca gttgcatcga cccgtcacct 17940 tggatttgcc ccctcctcct gctgctactg ctgtacccgc ttctaagcct gtcgctgccc 18000 cgaaaccagt cgccgtagcc aggtcacgtc ccgggggcgc tcctcgtcca aatgcacact 18060 ggcaaaatac tctgaacagc atcgtgggtc taggcgtgca aagtgtaaaa cgccgtcgct 18120 gcttttaatt aaatatggag tagcgcttaa cttgcctatc tgtgtatatg tgtcattaca 18180 cgccgtcaca gcatcagagg aaaaaaggaa gaggtcgtgc gtcgacgctg agttactttc 18240 aagatggcca ccccatcgat gctgccccaa tgggcataca tgcacatcgc cggacaggat 18300 gcttcggagt acctgagtcc gggtctggtg cagttcgccc gcgccacaga cacctacttc 18360 aatctgggaa ataagtttag aaatcctacc gtagcgccga cccacgatgt gaccaccgat 18420 cgtagccagc ggctcatgtt gcgcttcgtg cccgttgacc gggaggacaa tacatactct 18480 tacaaagtgc ggtacaccct ggccgtgggc gacaacagag tgctggatat ggccagcacg 18540 ttctttgaca ttaggggcgt gttggacaga ggtcccagtt ttaaacccta ttctggtacg 18600 gcttacaact ccctggctcc taaaggcgct ccaaatgcat ctcagtggtt ggataaggga 18660 gttacaagca ctggcctagt ggacgacggc aatactgatg atggggaaga agccaaaaaa 18720 gcaacataca cttttggtaa tgctccagta aaagccgagg ctgaaatcac aaaagacgga 18780 ttgccggtgg gcttggaagt ttcaactgaa ggtcctaaac caatctatgc tgataagctt 18840 tatcagccag aacctcaagt gggagacgaa acttggactg acctagacgg aaaaaccgaa 18900 gagtatggag ggagggttct taaacctgaa actaaaatga aaccctgcta cggatctttt 18960 gctaaaccta ctaatattaa aggaggtcag gcaaaggtaa aaccaaaaga agacgatggc 19020 actaacaaca tcgagtatga cattgacatg aacttctttg acttaagatc acaaagatca 19080 gaactcaaac ctaaaattgt aatgtatgca gaaaatgtgg acctggaatg tccagatact 19140 catgttgtgt acaaacctgg agtttcagat gctagttctg agaccaatct tggacaacag 19200 tctatgccca acagacccaa ctacattggc ttcagagata acttcatcgg acttatgtac 19260 tataacagta ctggcaacat gggggtactg gctggccaag cgtctcagtt gaatgcagtg 19320 gttgacttgc aggacagaaa cacagaactg tcttaccaac tcttgcttga ctctctgggc 19380 gacagaacca gatactttag catgtggaat caggctgtgg acagttatga tcctgatgta 19440 cgtgttattg aaaatcatgg tgtggaagat gaacttccca actattgttt tccgttggat 19500 ggtgtcggtc cgcgaacaga tagttacaag gagattaagc caaatggaga ccaatctact 19560 tggacaaatg tagacccaac tggcagcagt gaacttgcta agggaaatcc atttgccatg 19620 gaaattaacc ttcaagccaa tctatggcga agtttccttt attccaatgt ggctctatat 19680 ctcccagact cgtacaaata caccccgtcc aatgtcactc ttccagaaaa caaaaacacc 19740 tacgactaca tgaacgggcg ggtggtgccg ccatctctag tagacaccta tgtgaacatt 19800 ggtgccaggt ggtctctgga tgccatggac aatgtcaacc cattcaacca ccaccgtaac 19860 gctggcttgc gttaccgatc catgcttctg ggtaacggac gttatgtgcc tttccacata 19920 caagtgcctc aaaaattctt cgctgttaaa aacctgctgc ttctcccagg ctcctacact 19980 tatgagtgga actttaggaa ggatgtaaac atggttctac agagttccct cggtaacgac 20040 ctacgggtag atggcgccag catcagtttt acgagcatca acctctatgc tacttttttc 20100 cccatggctc acaacaccgc ttccaccctt gaagccatgc tgcggaatga caccaatgat 20160 cagtcattca acgactacct atctgcagct aacatgctct accccattcc tgccaatgca 20220 accaatattc ccatttccat tccttctcgc aactgggcgg ctttcagagg ctggtcattt 20280 accagactga aaaccaaaga aactccctct ttggggtctg gatttgaccc ctacttcgtc 20340 tattctggtt ctattcccta cctggatggt accttctacc tgaaccacac ttttaagaag 20400 gtttccatca tgtttgactc ttcagtgagc tggcctggaa atgacaggtt actatctcct 20460 aacgaatttg aaataaagcg cactgtggat ggcgaaggct acaacgtagc ccaatgcaac 20520 atgaccaaag actggttctt ggtacagatg ctcgccaact acaacatcgg ctatcagggc 20580 ttctacattc cagaaggata caaagatcgc atgtattcat ttttcagaaa cttccagccc 20640 atgagcaggc aggtggttga tgaggtcaat tacaaagact tcaaggccgt cgccataccc 20700 taccaacaca acaactctgg ctttgtgggt tacatggctc cgaccatgcg tcaaggtcaa 20760 ccctatcccg ctaactatcc ctatccactc attggaacaa ctgccgtaaa tagtgttacg 20820 cagaaaaagt tcttgtgtga cagaaccatg tggcgcatac cgttctcaag caacttcatg 20880 tctatgggag cccttacaga cttgggacag aacatgctct atgccaactc agctcatgct 20940 ctggacatga cctttgaggt ggatcccatg gatgagccca ccctgcttta tcttctcttc 21000 gaagttttcg acgtggtcag agtgcatcag ccacaccgcg gcatcatcga ggcagtctac 21060 ctgcgtacac cgttctcggc cggtaacgct accacgtaag aagcttcttg cttcttgcaa 21120 acagcagctg caaccatggc ctgcggatcc caaaacggct ccagcgagca agagctcaga 21180 gccattgtcc aagacctggg ttgcggacca tattttttgg gaacctttga taagcgcttc 21240 ccggggttca tggcccccga taagctcgcc tgtgccattg taaatacggc cggacgtgag 21300 acggggggag agcactggtt ggctttcggt tggaacccac gttctaacac ctgctacctt 21360 tttgatcctt ttggattctc ggatgatcgt ctcaaacaga tttaccagtt tgaatatgag 21420 ggtctcctgc gccgcagcgc tcttgctacc aaggaccggt gtattacgct ggaaaaatct 21480 acccagaccg tgcagggccc ccgttctgcc gcctgcggac ttttctgctg catgttcctt 21540 catgcctttg tgcactggcc tgaccgtccc atggacggaa accccaccat gaaattgcta 21600 actggagtgc caaacaacat gcttcattct cctaaagtcc agcccaccct gtgtgacaat 21660 caaaaagcac tctaccattt tctcaatacc cattcgcctt attttcgctc tcatcgtaca 21720 cacatcgaaa gggccactgc gttcgaccgt atggatgtgc aataatgatt catgtaaaca 21780 acgtgttcaa taaacagcac tttatttttt acatgtatcg aggctctgga ttacttattt 21840 atttacaagt cgaatgggtt ctgacgagaa tcagaatgac ccgcaggcag tgatacgttg 21900 cggaactgat acttgggttg ccacttgaat tcgggaatca ccaacttggg aaccggtata 21960 tcgggcagga tgtcactcca cagctttctg gtcagctgca aagctcccag caggtcagga 22020 gccgaaatct tgaaatcaca attaggacca gtgctctgag cgcgagagtt gcggtacacc 22080 ggattgcagc actgaaacac catcagcgac ggatgtctta cgcttgccag cacggtggga 22140 tctgcaatca tgcccacatc cagatcttca gcattggcaa tgctgaacgg ggtcatcttg 22200 caggtctgcc tacccatggc gggcacccaa ttaggcttgt ggttacaatc gcagtgcagg 22260 gggatcagta tcatcttggc ctgatcctgt ctgattcctg gatacacggc tctcatgaaa 22320 gcatcatatt gcttgaaagc ctgctgggct ttactaccct cggtataaaa catcccgcag 22380 gacctgctcg aaaactggtt agctgcgcag ccggcatcat tcacacagca gcgggcgtca 22440 ttgttggcta tttgcaccac acttctgccc cagcggtttt gggtgatttt ggttcgctcg 22500 ggattctcct tcaaggctcg ttgtccgttc tcgctggcca catccatctc gataatctgc 22560 tccttctgaa tcataatatt gccatgcaag cacttcagct tgccctcata atcattgcag 22620 ccatgaggcc acaacgcaca gcctgtacat tcccaattat ggtgggcgat ctgagaaaaa 22680 gaatgtatca ttccctgcag aaatcttccc atcatcgtgc tcagtgtctt gtgactagtg 22740 aaagttaact ggatgcctcg gtgctcctcg ttcacgtact ggtgacagat gcgcttgtat 22800 tgttcgtgct gctcaggcat tagtttaaaa gaggttctaa gttcgttatc cagcctgtac 22860 ttctccatca gcagacacat cacttccatg cctttctccc aagcagacac caggggcaag 22920 ctaatcggat tcttaacagt gcaggcagca gctcctttag ccagagggtc atctttggcg 22980 atcttctcaa tgcttctttt gccatccttc tcaacgatgc gcacgggcgg gtagctgaaa 23040 cccactgcta caagttgcgc ctcttctctt tcttcttcgc tgtcttgact gatgtcttgc 23100 atggggacat gtttggtctt ccttggcttc tttttcgggg gtatcggagg aggaggactg 23160 tcgctccgtt ccggagacag ggaggattgt gacgtttcgc tcaccattac caactgactg 23220 tcggtagaag aacctgaccc cacacggcga caggtgtttc tcttcggggg cagaggtgga 23280 ggcgattgcg aagggctgcg gtccgacctg gaaggcggat gactggcaga accccttccg 23340 cgttcggggg tgtgctccct gtggcggtcg cttaactgat ttccttcgcg gctggccatt 23400 gtgttctcct aggcagagaa acaacagaca tggaaactca gccattgctg tcaacatcgc 23460 cacgagtgcc atcacatctc gtcctcagcg acgaggaaaa ggagcagagc ttaagcattc 23520 caccgcccag tcctgccacc acctctaccc tagaagataa ggaggtcgac gcatctcatg 23580 acatgcagaa taaaaaagcg aaagagtctg agccagacat cgaacaagac ccgggctatg 23640 tgacaccggt ggaacacgag gaagagttga aacgctttct agagagagag gatgaaaact 23700 gcccaaaaca gcaagcggat aactatcacc aagatgctgg aaatagggat cagaacaccg 23760 actacctcat agggcttgac ggggaagacg cgctccttaa acatctagca agacagtcac 23820 tcatagtcaa ggatgcatta ttggacagaa ctgaagtgcc catcagtgtc gaagagctca 23880 gccgcgccta cgagcttaac ctattttcac ctcgtactcc ccccaaacgt cagccaaacg 23940 gcacctgcga gccaaatcct cgcttaaact tttatccagc ttttgctgtg ccagaagtac 24000 tggctaccta tcacatcttt tttaaaaatc aaaaaattcc agtctcctgc cgcgctaatc 24060 gcacccgcgc cgatgcccta ctcaatctgg gacctggttc acgcttacct gatatagctt 24120 ccttggaaga ggttccaaag atcttcgagg gtctgggcaa taatgagact cgggccgcaa 24180 atgctctgca aaagggagaa aatggcatgg atgagcatca cagcgttctg gtggaattgg 24240 aaggcgataa tgccagactc gcagtactca agcgaagcgt cgaggtcaca cactttgcat 24300 accccgctgt caacctgccc cctaaagtca tgacggccgt catggaccag ttactcatta 24360 agcgcgcaag tcccctttca gaagacatgc atgacccaga tgcctgtgat gagggtaaac 24420 cagtggtcag tgatgagcag ctaacccgat ggctgggcac cgactctccc cgggatttgg 24480 aagagcgtcg caagcttatg atggccgtgg tgctggttac cgtagaacta gagtgtcttc 24540 ggcgtttctt taccgattca gaaaccttgc gcaaactcga agagaatctg cactacactt 24600 ttagacacgg ctttgtgcgg caggcatgca agatatctaa cgtggaactc accaacctgg 24660 tttcctacat gggtattctg catgagaatc gcctaggaca aagcgtgctg cacagcaccc 24720 ttaaggggga agcccgccgt gattacatcc gcgattgtgt ttatctctac ctgtgccaca 24780 cgtggcaaac cggcatgggt gtatggcagc aatgtttaga agaacagaac ctgaaagagc 24840 taaacaagct cttacagaaa tctcttaagg ttctgtggac agggttcgac gagcgcaccg 24900 tcgcttccga cctggcagac ctcatcttcc cagagcgtct cagggttact ttgcgaaacg 24960 gactgcctga ctttatgagc cagagcatgc ttaacaattt tcgctctttc atcctggaac 25020 gctccggtat cctgcccgcc acctgctgcg cactgccctc cgactttgtg cctctcacct 25080 accgcgaatg ccccccgccg ctatggagtc actgctacct gttccgtctg gccaactacc 25140 tctcctacca ctcggatgtg atcgaggatg tgagcggaga cggcttgctg gagtgtcact 25200 gccgctgcaa tctgtgcacg ccccaccggt ccctagcttg caacccccag ttgatgagcg 25260 aaacccagat aataggcacc tttgaattgc aaggccccag cagccaaggc gatgggtctt 25320 ctcctgggca aagtttaaaa ctgaccccgg gactgtggac ctccgcctac ttgcgcaagt 25380 ttgccccgga agattaccac ccctatgaaa tcaagttcta tgaggaccaa tcacagcctc 25440 cgaaagccga actttcggcc tgcgtcatca cccagggggc aattctggcc caattgcaag 25500 ccatccaaaa atcccgccaa gaatttctac tgaaaaaggg taagggggtc taccttgacc 25560 cccagaccgg cgaggaactc aacacaaggt tccctcagga tgtcccaacg acgagaaagc 25620 aagaagttga aggtgcagcc gccgccccca gaagatatgg aggaagattg ggacagtcag 25680 gcagaggaag cggaggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740 gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800 gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860 cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920 ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980 tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040 ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100 agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160 accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220 agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280 catcttccag cagagtcggg gccaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340 ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400 ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460 cgcgcttatt caaaaaaggc gggaattaca tcatcctcga cgtttatgag taaagaaatt 26520 cccacgcctt acatgtggag ttatcagccc caaatgggat tggcggcagg cgcctcccag 26580 gactactcca cccgcatgaa ttggctcagc gccgggcctt ctatgatttc tcgagttaat 26640 gatatacgcg cctaccgaaa ccaaatactt ttggaacagt cagctcttac caccacgccc 26700 cgccaacacc ttaatcccag aaattggccc gccgccctag tgtaccagga aagtcccgct 26760 cccaccactg tattacttcc tcgagacgcc caggccgaag tccaaatgac taatgcaggt 26820 gcgcagttag cgggcggctc caccctatgt cgtcacaggc ctcggcataa tataaaacgc 26880 ctgatgatca gaggccgagg tatccagctc aacgacgagt cggtgagctc tccgcttggt 26940 ctacgaccag acggaatctt tcagattgcc ggctgcggga gatcttcctt cacccctcgt 27000 caggctgttc tgactttgga aagttcgtct tcgcaacccc gctcgggcgg aatcgggacc 27060 gttcaatttg tggaggagtt tactccctct gtctacttca accccttctc cggatctcct 27120 gggcactacc cggacgagtt cataccgaac ttcgacgcga ttagcgagtc agtggacggc 27180 tacgattgat gtctggtgac gcggctgagc tatctcggct gcgacatcta gaccactgcc 27240 gccgctttcg ctgctttgcc cgggaactca ttgagttcat ctacttcgaa ctccccaagg 27300 atcaccctca aggtccggcc cacggagtgc ggattactat cgaaggcaaa atacactctc 27360 gcctgcaacg aattttctcc cagcggcccg tgctgatcga gcgagaccag ggaaacacca 27420 cggtttccat ctactgcatt tgtaatcacc ccggattgca tgaaagcctt tgctgtctta 27480 tgtgtactga gtttaataaa aactgaatta agactctcct acggactgcc gcttcttcaa 27540 cccggatttt acaaccagaa gaacgaaact tttcctgtcg tccaggactc tgttaacttc 27600 acctttccta ctcacaaact agaagctcaa cgactacacc gcttttccag aagcattttc 27660 cctactaata ctactttcaa aaccggaggt gagctccaag gtcttcctac agaaaaccct 27720 tgggtggaag cgggccttgt agtgctagga attcttgcgg gtgggcttgt gattattctt 27780 tgctacctat acacaccttg cttcactttc ctagtggtgt tgtggtattg gtttaaaaaa 27840 tggggcccat actagtcttg cttgttttac tttcgctttt ggaaccgggt tctgccaatt 27900 acgatccatg tctagacttc gacccagaaa actgcacact tacttttgca cccgacacaa 27960 gccgcatctg tggagttctt attaagtgcg gatgggaatg caggtccgtt gaaattacac 28020 acaataacaa aacctggaac aataccttat ccaccacatg ggagccagga gttcccgagt 28080 ggtacactgt ctctgtccga ggtcctgacg gttccatccg cattagtaac aacactttca 28140 ttttttctga aatgtgcgat ctggccatgt tcatgagcaa acagtattct ctatggcctc 28200 ctagcaagga caacatcgta acgttctcca ttgcttattg cttgtgcgct tgccttctta 28260 ctgctttact gtgcgtatgc atacacctgc ttgtaaccac tcgcatcaaa aacgccaata 28320 acaaagaaaa aatgccttaa cctctttctg tttacagaca tggcttctct tacatctctc 28380 atatttgtca gcattgtcac tgccgctcac ggacaaacag tcgtctctat ccctctagga 28440 cataattaca ctctcatagg acccccaatc acttcagagg tcatctggac caaactggga 28500 agcgttgatt actttgatat aatctgcaac aaaacaaaac caataatagt aacttgcaac 28560 atacaaaatc ttacattgat taatgttagc aaagtttaca gcggttacta ttatggttat 28620 gacagataca gtagtcaata tagaaattac ttggttcgtg ttacccagtt aaaaaccacg 28680 aaaatgccaa atatggcaaa gattcgatcc gatgacaatt ctctagaaac ttttacatct 28740 cccaccacac ccgacgaaaa aaacatccca gattcaatga ttgcaattgt tgcagcggtg 28800 gcagtggtga tggcactaat aataatatgc atgcttttat atgcttgtcg ctacaaaaag 28860 tttcatccta aaaaacaaga tctcctacta aggcttaaca tttaatttct ttttatacag 28920 ccatggtttc cactaccaca ttccttatgc ttactagtct tgcaactctg acttctgctc 28980 gctcacacct cactgtaact ataggctcaa actgcacact aaaaggacct caaggtggtc 29040 atgtcttttg gtggagaata tatgacaatg gatggtttac aaaaccatgt gaccaacctg 29100 gtagattttt ctgcaacggc agagacctaa ccattatcaa cgtgacagca aatgacaaag 29160 gcttctatta tggaaccgac tataaaagta gtttagatta taacattatt gtactgccat 29220 ctaccactcc agcaccccgc acaactactt tctctagcag cagtgtcgct aacaatacaa 29280 tttccaatcc aacctttgcc gcgcttttaa aacgcactgt gaataattct acaacttcac 29340 atacaacaat ttccacttca acaatcagca ttatcgctgc agtgacaatt ggaatatcta 29400 ttcttgtttt taccataacc tactacgcct gctgctatag aaaagacaaa cataaaggtg 29460 atccattact tagatttgat atttaatttg ttcttttttt ttttatttac agtatggtga 29520 acaccaatca tggtacctag aaatttcttc ttcaccatac tcatttgtgc atttaatgtt 29580 tgcgctactt tcacagcagt agccacagca accccagact gtataggagc atttgcttcc 29640 tatgcacttt ttgcttttgt tacttgcatc tgcgtatgta gcatagtctg cctggttatt 29700 aattttttcc aacttctaga ctggatcctt gtgcgaattg cctacctgcg ccaccatccc 29760 gaataccgca accaaaatat cgcggcactt cttagactca tctaaaacca tgcaggctat 29820 actaccaata tttttgcttc tattgcttcc ctacgctgtc tcaaccccag ctgcctatag 29880 tactccacca gaacacctta gaaaatgcaa attccaacaa ccgtggtcat ttcttgcttg 29940 ctatcgagaa aaatcagaaa ttcccccaaa tttaataatg attgctggaa taattaatat 30000 aatctgttgc accataattt catttttgat atacccccta tttgattttg gctggaatgc 30060 tcccaatgca catgatcatc cacaagaccc agaggaacac attcccctac aaaacatgca 30120 acatccaata gcgctaatag attacgaaag tgaaccacaa cccccactac tccctgctat 30180 tagttacttc aacctaaccg gcggagatga ctgaaacact caccacctcc aattccgccg 30240 aggatctgct cgatatggac ggccgcgtct cagaacagcg acttgcccaa ctacgcatcc 30300 gccagcagca ggaacgcgcg gccaaagagc tcagagatgt catccaaatt caccaatgca 30360 aaaaaggcat attctgtttg gtaaaacaag ccaagatatc ctacgagatc accgctactg 30420 accatcgcct ctcttacgaa cttggccccc aacgacaaaa atttacctgc atggtgggaa 30480 tcaaccccat agttatcacc cagcaaagtg gagatactaa gggttgcatt cactgctcct 30540 gcgattccat cgagtgcacc tacaccctgc tgaagaccct atgcggccta agagacctgc 30600 taccaatgaa tggaagcgga gctactaact tcagcctgct gaagcaggct ggtgacgtcg 30660 aggagaatcc tggcccaatg gtgagcaaag gcgaagagct gttcaccggc gtggtgccca 30720 tcctggtgga gctggacggc gacgtgaacg gccacaagtt cagcgtgagc ggcgagggcg 30780 agggcgacgc cacctacggc aagctgaccc tgaagctgct gtgcaccacc ggcaagctgc 30840 ccgtgccctg gcccaccctg gtgaccaccc tgggctacgg cgtgcagtgc ttcgcccggt 30900 accccgacca catgaagcag cacgacttct tcaagagcgc catgcccgag ggctacgtgc 30960 aggagcggac catcttcttc aaggacgacg gcaactacaa gacccgggcc gaggtgaagt 31020 tcgagggcga caccctggtg aaccggatcg agctgaaggg catcgacttc aaggaggacg 31080 gcaacatcct gggccacaag ctggagtaca actacaacag ccacaacgtg tacatcaccg 31140 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccggcacaac atcgaggacg 31200 gcggcgtgca gctggccgac cactaccagc agaacacccc catcggcgac ggccccgtgc 31260 tgctgcccga caaccactac ctgagctacc agagcgccct gttcaaggac cccaacgaga 31320 agcgggacca catggtgctg ctggagttcc tgaccgccgc cggcatcacc gagggcatga 31380 acgagctcta taagtaaaaa atgattaata aaaaatcact tacttgaaat cagcaataag 31440 gtctctgttg aaattttctc ccagcagcac ctcacttccc tcttcccaac tctggtattc 31500 taaaccccgt tcagcggcat actttctcca tactttaaag gggatgtcaa attttagctc 31560 ctctcctgta cccacaatct tcatgtcttt cttcccagat gaccaagaga gtccggctca 31620 gtgactcctt caaccctgtc tacccctatg aagatgaaag cacctcccaa caccccttta 31680 taaacccagg gtttatttcc ccaaatggct tcacacaaag cccagacgga gttcttactt 31740 taaaatgttt aaccccacta acaaccacag gcggatctct acagctaaaa gtgggagggg 31800 gacttacagt ggatgacact gatggtacct tacaagaaaa catacgtgct acagcaccca 31860 ttactaaaaa taatcactct gtagaactat ccattggaaa tggattagaa actcaaaaca 31920 ataaactatg tgccaaattg ggaaatgggt taaaatttaa caacggtgac atttgtataa 31980 aggatagtat taacacctta tggactggaa taaaccctcc acctaactgt caaattgtgg 32040 aaaacactaa tacaaatgat ggcaaactta ctttagtatt agtaaaaaac ggagggcttg 32100 ttaatggcta cgtgtctcta gttggtgtat cagacactgt gaaccaaatg ttcacacaaa 32160 agacagcaaa catccaatta agattatatt ttgactcttc tggaaatcta ttaactgatg 32220 aatcagactt aaaaattcca cttaaaaata aatcttctac agcgaccagt gaaactgtag 32280 ccagcagcaa agcctttatg ccaagtacta cagcttatcc cttcaacacc actactaggg 32340 atagtgaaaa ctacattcat ggaatatgtt actacatgac tagttatgat agaagtctat 32400 ttcccttgaa catttctata atgctaaaca gccgtatgat ttcttccaat gttgcctatg 32460 ccatacaatt tgaatggaat ctaaatgcaa gtgaatctcc agaaagcaac atagctacgc 32520 tgaccacatc cccctttttc ttttcttaca ttacagaaga cgacaactaa aataaagttt 32580 aagtgttttt atttaaaact cacaaaattc gagtagttat tttgcctcca ccttcccatt 32640 tgacagaata caccaatctc tccccacgca cagctttaaa catttggata ccattagaga 32700 tagacattgt tttagattcc acattccaaa cagtttcaga gcgagccaat ctggggtcag 32760 tgatagataa aaatccatcg cgatagtctt ttaaagcgct ttcacagtcc aactgctgcg 32820 gatgcgaatc cggagtctgg atcacggtca tctggaagaa gaacgatggg aatcataatc 32880 cgaaaacggt atcggacgat tgtgtctcat caaacccaca agcagccgct gtctgcgtcg 32940 ctccgtgcaa ctgctgttta tgggatcagg gtccacagtg tcctgaagca tgattttaat 33000 agcccttaac atcaactttc tggtgcgatg cgcgcagcaa cgcattctga tttcactcaa 33060 atctttgcag taggtacaac acattattac aatattgttt aataaaccat aattaaaagc 33120 gctccagcca aaactcatat ctgatataat cgcccctgca tgaccatcat accaaagttt 33180 aatataaatt aaatgacgtt ccctcaaaaa cacactaccc acatacatga tctcttttgg 33240 catgtgcata ttaacaatct gtctgtacca tggacaacgt tggttaatca tgcaacccaa 33300 tataaccttc cggaaccaca ctgccaacac cgctccccca gccatgcatt gaagtgaacc 33360 ctgctgatta caatgacaat gaagaaccca attctctcga ccgtgaatca cttgagaatg 33420 aaaaatatct atagtggcac aacatagaca taaatgcatg catcttctca taatttttaa 33480 ctcctcagga tttagaaaca tatcccaggg aataggaagc tcttgcagaa cagtaaagct 33540 ggcagaacaa ggaagaccac gaacacaact tacactatgc atagtcatag tatcacaatc 33600 tggcaacagc gggtggtctt cagtcataga agctcgggtt tcattttcct cacaacgtgg 33660 taactgggct ctggtgtaag ggtgatgtct ggcgcatgat gtcgagcgtg cgcgcaacct 33720 tgtcataatg gagttgcttc ctgacattct cgtattttgt atagcaaaac gcggccctgg 33780 cagaacacac tcttcttcgc cttctatcct gccgcttagc gtgttccgtg tgatagttca 33840 agtacagcca cactcttaag ttggtcaaaa gaatgctggc ttcagttgta atcaaaactc 33900 catcgcatct aattgttctg aggaaatcat ccacggtagc atatgcaaat cccaaccaag 33960 caatgcaact ggattgcgtt tcaagcagga gaggagaggg aagagacgga agaaccatgt 34020 taatttttat tccaaacgat ctcgcagtac ttcaaattgt agatcgcgca gatggcatct 34080 ctcgccccca ctgtgttggt gaaaaagcac agctaaatca aaagaaatgc gattttcaag 34140 gtgctcaacg gtggcttcca acaaagcctc cacgcgcaca tccaagaaca aaagaatacc 34200 aaaagaagga gcattttcta actcctcaat catcatatta cattcctgca ccattcccag 34260 ataattttca gctttccagc cttgaattat tcgtgtcagt tcttgtggta aatccaatcc 34320 acacattaca aacaggtccc ggagggcgcc ctccaccacc attcttaaac acaccctcat 34380 aatgacaaaa tatcttgctc ctgtgtcacc tgtagcgaat tgagaatggc aacatcaatt 34440 gacatgccct tggctctaag ttcttcttta agttctagtt gtaaaaactc tctcatatta 34500 tcaccaaact gcttagccag aagccccccg ggaacaagag caggggacgc tacagtgcag 34560 tacaagcgca gacctcccca attggctcca gcaaaaacaa gattggaata agcatattgg 34620 gaaccgccag taatatcatc gaagttgctg gaaatataat caggcagagt ttcttgtaaa 34680 aattgaataa aagaaaaatt tgccaaaaaa acattcaaaa cctctgggat gcaaatgcaa 34740 taggttaccg cgctgcgctc caacattgtt agttttgaat tagtctgcaa aaataaaaaa 34800 aaaaacaagc gtcatatcat agtagcctga cgaacaggtg gataaatcag tctttccatc 34860 acaagacaag ccacagggtc tccagctcga ccctcgtaaa acctgtcatg gtgattaaac 34920 aacagcaccg aaagttcctc gcggtgacca gcatgaataa ttcttgatga agcatacaat 34980 ccagacatgt tagcatcagt taacgagaaa aaacagccaa catagccttt gggtataatt 35040 atgcttaatc gtaagtatag caaagccacc cctcgcggat acaaagtaaa aggcacagga 35100 gaataaaaaa tataattatt tctctgctgc tgttcaggca acgtcgcccc cggtccctct 35160 aaatacacat acaaagcctc atcagccatg gcttaccaga caaagtacag cgggcacgca 35220 caagctctaa agtcactctc caacctctcc acaatatata tacacaagcc ctaaactgac 35280 gtaatgggag taaagtgtaa aaaatcccgc caaacccaac acacaccccg aaactgcgtc 35340 accagggaaa agtacagttt cacttccgca atcccaacaa gcgtcacttc ctctttctca 35400 cggtacgtca catcccatta acttgcaacg tcattttccc acggccgcgc cgccccgttt 35460 agccgttaac cccacagcca atcaccacac accccacaat ttttaaaatc acctcattta 35520 catattggca ccattccatc tataaggtat attattgatg atg 35563

Claims (39)

  1. 이종의(heterologous) 오픈 리딩 프레임(open reading frame, ORF) 및 자가-절단(self-cleaving) 펩타이드 암호화 서열을 포함하고, 둘은 작동 가능하게 연결되고 내인성(endogenous) 아데노바이러스 ORF와 동일한 리딩 프레임 내에 있으며, 상기 자가-절단 펩타이드는 2A 펩타이드 또는 이의 N-말단에 Gly-Ser-Gly를 포함하는 변이체고, 상기 자기-절단 펩타이드 암호화 서열은 상기 이종의 ORF와 상기 내인성 ORF 사이에 위치하는 재조합 아데노바이러스 게놈, 여기서:
    상기 내인성 ORF는 E1B-55k이고 상기 이종의 ORF는 E1B-55k의 3'에 삽입됨;
    상기 내인성 ORF는 DNA 중합효소(polymerase)이고 상기 이종의 ORF는 DNA 중합효소의 5'에 삽입됨;
    상기 내인성 ORF는 DNA-결합 단백질(DNA-binding protein (DBP))이고 상기 이종의 ORF는 DBP의 3'에 삽입됨;
    상기 내인성 ORF는 아데노바이러스 사멸 단백질(adenovirus death protein(ADP))이고 상기 이종의 ORF는 ADP의 5'에 삽입됨;
    상기 내인성 ORF는 E3-14.7k이고 상기 이종의 ORF는 E3-14.7k의 3'에 삽입됨; 또는
    상기 내인성 ORF는 E4-ORF2이고 상기 이종의 ORF는 E4-ORF2의 5'에 삽입됨.
  2. 제1항에 있어서,
    상기 이종의 ORF는 치료용 단백질(therapeutic protein)을 암호화하는, 재조합 아데노바이러스 게놈.
  3. 제2항에 있어서,
    상기 치료용 단백질은 사이토카인(cytokine), 케모카인(chemokine), T 세포 활성화 리간드 (T cell activating ligand), 동시-자극분자 (co-stimulatory molecule), 및 면역관문차단 억제제(immune checkpoint blockade inhibitor)로 구성된 군에서 선택되는 면역조절제(immunomodulator)를 포함하는, 재조합 아데노바이러스 게놈.
  4. 제1항에 있어서,
    상기 이종의 ORF는 형광 단백질(fluorescent protein)을 암호화하는, 재조합 아데노바이러스 게놈.
  5. 제4항에 있어서,
    상기 내인성 ORF는 ADP이고, 상기 이종의 ORF는 형광 단백질을 암호화하며, 자가-절단 펩타이드를 암호화하는 서열은 ADP의 5'에 삽입되는, 재조합 아데노바이러스 게놈.
  6. 제5항에 있어서, 상기 형광 단백질은 YPet인, 재조합 아데노바이러스 게놈.
  7. 제6항에 있어서,
    상기 2A 펩타이드는 돼지 테스코바이러스-1(porcine teschovirus-1 (PTV1)) 2A (P2A) 펩타이드 또는 이의 N-말단에 Gly-Ser-Gly를 포함하는 변이체, 수족구병(foot and mouth disease virus(FMDV) 2A (F2A) 펩타이드 또는 이의 N-말단에 Gly-Ser-Gly를 포함하는 변이체, 마 비염 A 바이러스(equine rhinitis A virus(ERAV)) 2A (E2A) 펩타이드 또는 이의 N-말단에 Gly-Ser-Gly를 포함하는 변이체, 또는 토세어 아시그나 바이러스(Thosea asigna virus(TaV)) 2A (T2A) 펩타이드 또는 이의 N-말단에 Gly-Ser-Gly를 포함하는 변이체인, 재조합 아데노바이러스 게놈.
  8. 제7항에 있어서, 상기 2A 펩타이드는 P2A인, 재조합 아데노바이러스 게놈.
  9. 제1항에 있어서,
    상기 2A 펩타이드는 돼지 테스코바이러스-1(porcine teschovirus-1 (PTV1)) 2A (P2A) 펩타이드 또는 이의 N-말단에 Gly-Ser-Gly를 포함하는 변이체, 수족구병(foot and mouth disease virus(FMDV) 2A (F2A) 펩타이드 또는 이의 N-말단에 Gly-Ser-Gly를 포함하는 변이체, 마 비염 A 바이러스(equine rhinitis A virus(ERAV)) 2A (E2A) 펩타이드 또는 이의 N-말단에 Gly-Ser-Gly를 포함하는 변이체, 또는 토세어 아시그나 바이러스(Thosea asigna virus(TaV)) 2A (T2A) 펩타이드 또는 이의 N-말단에 Gly-Ser-Gly를 포함하는 변이체인, 재조합 아데노바이러스 게놈.
  10. 제2항에 있어서,
    상기 2A 펩타이드는 돼지 테스코바이러스-1(porcine teschovirus-1 (PTV1)) 2A (P2A) 펩타이드 또는 이의 N-말단에 Gly-Ser-Gly를 포함하는 변이체, 수족구병(foot and mouth disease virus(FMDV) 2A (F2A) 펩타이드 또는 이의 N-말단에 Gly-Ser-Gly를 포함하는 변이체, 마 비염 A 바이러스(equine rhinitis A virus(ERAV)) 2A (E2A) 펩타이드 또는 이의 N-말단에 Gly-Ser-Gly를 포함하는 변이체, 또는 토세어 아시그나 바이러스(Thosea asigna virus(TaV)) 2A (T2A) 펩타이드 또는 이의 N-말단에 Gly-Ser-Gly를 포함하는 변이체인, 재조합 아데노바이러스 게놈.
  11. 제3항에 있어서, 상기 자가-절단 펩타이드는 2A 펩타이드 또는 이의 N-말단에 Gly-Ser-Gly를 포함하는 변이체인, 재조합 아데노바이러스 게놈.
  12. 제4항에 있어서,
    상기 2A 펩타이드는 돼지 테스코바이러스-1(porcine teschovirus-1 (PTV1)) 2A (P2A) 펩타이드 또는 이의 N-말단에 Gly-Ser-Gly를 포함하는 변이체, 수족구병(foot and mouth disease virus(FMDV) 2A (F2A) 펩타이드 또는 이의 N-말단에 Gly-Ser-Gly를 포함하는 변이체, 마 비염 A 바이러스(equine rhinitis A virus(ERAV)) 2A (E2A) 펩타이드 또는 이의 N-말단에 Gly-Ser-Gly를 포함하는 변이체, 또는 토세어 아시그나 바이러스(Thosea asigna virus(TaV)) 2A (T2A) 펩타이드 또는 이의 N-말단에 Gly-Ser-Gly를 포함하는 변이체인, 재조합 아데노바이러스 게놈.
  13. 제9항에 있어서,
    상기 자가-절단 펩타이드의 아미노산 서열은 서열번호 14 내지 21 중 어느 하나의 아미노산 서열과 적어도 80% 동일한, 재조합 아데노바이러스 게놈.
  14. 제9항에 있어서,
    상기 자가-절단 펩타이드는 서열번호 14 내지 21 중 어느 하나의 아미노산 서열을 포함하는, 재조합 아데노바이러스 게놈.
  15. 재조합 아데노바이러스 게놈이 Ad5 게놈인, 제1항 내지 제12항 중 어느 한 항의 재조합 아데노바이러스 게놈.
  16. 제 15항에 있어서,
    게놈은 Ad5의 파이버 샤프트 (fiber shaft) 및 아데노바이러스의 제2 항원형의 파이버 노브 (fiber knob)를 포함하는 키메라 파이버 단백질을 암호화하는 것인, 재조합 아데노바이러스 게놈.
  17. 제16항에 있어서,
    아데노바이러스의 제2 항원형은 Ad9 또는 Ad34인, 재조합 아데노바이러스 게놈.
  18. E4orf6/7 단백질 코딩 서열이 삭제된, 제1항 내지 제12항 중 어느 한 항의 재조합 아데노바이러스 게놈.
  19. 제8항에 있어서,
    재조합 아데노바이러스 게놈은 Ad5 게놈이고, 게놈이 Ad5로부터 파이버 샤프트 및 아데노바이러스 Ad9 또는 Ad34로부터의 파이버 노브를 포함하는 키메라 파이버 단백질을 코딩하고, 여기서 4orf6/7 단백질 코딩 서열이 삭제된, 재조합 아데노바이러스 게놈.
  20. 제1항 내지 제14항 또는 제19항 중 어느 한 항의 재조합 아데노바이러스 및 약학적으로 허용 가능한 담체를 포함하는 종양 세포 생존율 또는 종양 세포 성장 억제용 조성물.
  21. 재조합 아데노바이러스의 게놈이 제1항 내지 제14항 또는 제19항 중 어느 한 항의 재조합 아데노바이러스 게놈으로 구성되는 것인, 재조합 아데노바이러스.
  22. 제21항의 재조합 아데노바이러스 및 약학적으로 허용가능한 담체를 포함하는 종양 세포 생존율 또는 종양 세포 성장을 억제용 조성물.
  23. 제21항의 재조합 아데노바이러스 및 약학적으로 허용가능한 담체를 포함하는 종양 진행 억제용 또는 종양 부피 감소용 조성물.
  24. 제21항의 재조합 아데노바이러스 및 약학적으로 허용가능한 담체를 포함하는 암 치료용 조성물.
  25. 제24항에 있어서,
    치료제를 추가로 포함하는 조성물.
  26. (i) 제21항의 재조합 아데노바이러스 및 약학적으로 허용 가능한 담체를 포함하는 조성물; 및
    (ii) 하나 이상의 치료제 또는 하나 이상의 진단제를 포함하는, 키트
  27. 제26항에 있어서,
    상기 하나 이상의 치료제는 화학요법(chemotherapeutic), 생물학적(biologic), 또는 이의 조합을 포함하는, 키트.
  28. 제26항에 있어서,
    상기 하나 이상의 진단제는 종양 마커에 특이적인 하나 이상의 항체를 포함하는, 키트.
  29. 제26항에 있어서,
    상기 하나 이상의 진단제는 종양 마커에 특이적인 하나 이상의 핵산 분자를 포함하는, 키트.
  30. 제18항의 재조합 아데노바이러스 및 약학적으로 허용 가능한 담체를 포함하는 종양 세포 생존율 또는 종양 세포 성장 억제용 조성물.
  31. 재조합 아데노바이러스의 게놈이 제18항 의 재조합 아데노바이러스 게놈으로 구성되는 것인, 재조합 아데노바이러스.
  32. 제31항의 재조합 아데노바이러스 및 약학적으로 허용가능한 담체를 포함하는 종양 세포 생존율 또는 종양 세포 성장을 억제용 조성물.
  33. 제31항의 재조합 아데노바이러스 및 약학적으로 허용가능한 담체를 포함하는 종양 진행 억제용 또는 종양 부피 감소용 조성물.
  34. 제31항의 재조합 아데노바이러스 및 약학적으로 허용가능한 담체를 포함하는 암 치료용 조성물.
  35. 제34항에 있어서,
    치료제를 추가로 포함하는 조성물.
  36. (i) 제31항의 재조합 아데노바이러스 및 약학적으로 허용 가능한 담체를 포함하는 조성물; 및
    (ii) 하나 이상의 치료제 또는 하나 이상의 진단제를 포함하는, 키트
  37. 제36항에 있어서,
    상기 하나 이상의 치료제는 화학요법(chemotherapeutic), 생물학적(biologic), 또는 이의 조합을 포함하는, 키트.
  38. 제36항에 있어서,
    상기 하나 이상의 진단제는 종양 마커에 특이적인 하나 이상의 항체를 포함하는, 키트.
  39. 제36항에 있어서,
    상기 하나 이상의 진단제는 종양 마커에 특이적인 하나 이상의 핵산 분자를 포함하는, 키트.
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