CN1046763C - 抗除草剂的嵌合基因 - Google Patents

抗除草剂的嵌合基因 Download PDF

Info

Publication number
CN1046763C
CN1046763C CN89101778A CN89101778A CN1046763C CN 1046763 C CN1046763 C CN 1046763C CN 89101778 A CN89101778 A CN 89101778A CN 89101778 A CN89101778 A CN 89101778A CN 1046763 C CN1046763 C CN 1046763C
Authority
CN
China
Prior art keywords
gene
small subunit
site
rubisco
district
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN89101778A
Other languages
English (en)
Other versions
CN1036038A (zh
Inventor
伯纳德·雷诺克斯
伯纳德·佩利西耶
米歇尔·勒布伦
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ronnie Planck Agricultural Chemical Co
Bayer CropScience SA
Original Assignee
Ronnie Planck Agricultural Chemical Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ronnie Planck Agricultural Chemical Co filed Critical Ronnie Planck Agricultural Chemical Co
Publication of CN1036038A publication Critical patent/CN1036038A/zh
Application granted granted Critical
Publication of CN1046763C publication Critical patent/CN1046763C/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Thermistors And Varistors (AREA)
  • Glass Compositions (AREA)
  • Compositions Of Oxide Ceramics (AREA)
  • Catching Or Destruction (AREA)
  • Saccharide Compounds (AREA)
  • Jellies, Jams, And Syrups (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

本发明涉及使植物对除草剂具有抗性的基因。使植物对3，5-二卤代-4-羟基苄腈类除草剂具有抗性的嵌合基因，它包括至少一种能编码抗这类除草剂的基因，一种外来启动子和选择性地，一个多聚腺苷化信号区，其中启动于来自于在植物细胞中自然表达的基因；它选自由花椰菜镶嵌病毒(Ca MV 35 S)的35S RNA的启动子和向日葵(Helianthus annuus)核酮糖-1，5-双磷酸羧酶/加氧酶(Rubis CO)的小亚基(SSU)的启动子所组成的组及其农业上的应用。

Description

抗除草剂的嵌合基因
本发明涉及能够使植物对于3,5-二卤代-4-羟基苄腈类除草剂具有抗性的一种新的嵌合基因，以及涉及用该基因转化植物细胞的方法和由这类细胞再生的转化植物。
业已知道，第229,042号欧洲专利申请揭示了通过将一种编码可专一地降解这类除草剂的腈水解酶的基因导入植物的基因组，从而产生对上述除草剂，尤其是对3,5-二溴-4-苄腈或溴苯腈具有抗性的植物。尽管该技术取得了有效的结果，但为了增加成功的机会和增强经济效果，它还需要进行改进，尤其是在植物中的表达水平和相应的植物抗这些除草剂的能力方面。
在本说明书中，“植物”可理解为能进行光合作用的任何分化型多细胞有机体，“植物细胞”意指来自植物的、能够形成不分化的组织(如胼胝体或胚胎)或分化的组织(如植物的部分或植物或种子)的任何细胞。
本发明的目的是满足这种需要。
本发明涉及能够使植物对3,5-二卤代-4-羟基苄腈类除草剂具有抗性的嵌合基因，它包括至少一种使植物对这类除草剂产生抗性的腈水解酶的基因，一种外源启动子或者，一个多聚腺苷化信号区，其中启动子来源于在植物细胞中自然表达的基因，它选自由花椰菜镶嵌病毒(CaMV35S)的35SRNA的启动子和向日葵(Helianthus annuus)核酮糖-1,5-双磷酸羧酶/加氧酶(Rubis CO)的小亚基(SSU)的启动子所组成的组。
本发明的嵌合基因的启动子来源于在植物中自然表达的基因，即非植物型，如病毒型，诸如花椰菜镶嵌病毒(CaMV35S)，或者较佳地是植物型，如单子叶或双子叶植物，尤其是向日葵(Helianthus annuus)核酮糖-1,5-二磷酸羧酶/加氧酶(Rubis CO)的小亚基。单独地或结合地使用这些启动子都是可能的。其选择依赖于所要转化的单或双子叶植物的性质。因此，使用用于双子叶植物转化的向日葵小亚基Rubis CO更好。
每个启动子按下列方法获得：
(1)花椰菜镶嵌病毒(CaMV35S)的35S RNA的启动子：
该启动子的分离如Odell等人(1985)所述。含有转录启始位点上游约850bp(碱基对)的克隆(P J05-2)被选出用以上述构造。将分离到的EcoRⅠ-HindⅢ片段，用Klenoa聚合酶将其末端补平，并克隆到载体pUC19的HincⅡ位点上(Yannishperron等人，1985)中。用XbaⅠ和PstⅠ消化该克隆，月噬菌体T4聚合酶处理所得到的片段以使其末端补平(blunt)。将该片段克隆进用SmaⅠ和XbaⅠ酶切并用Klenow聚合酶处理的pUC19Cm(Buckley,1985)中，由此而得到的克隆命名为pRPA-BL145。将用Klenow聚合酶处理的3′末端AccⅠ位点，与位于该启动子下游片段中用Klenow聚合酶处理过的EcoRⅠ位点相连接，重新形成EcoRⅠ位点并由此而获得顺序，从转录启始位点开始呈如下形式：
ACACGCTGACAAGCTGACTCAGCTAGAGTCGAATTC
EcoRⅠ
2)向日葵(Helianthus annuus)核酮糖-1,5-双磷酸羧酶/加氧酶(Rubis CO)的小亚基的启动子。
含有该启动子的基因已经由Wacksmon等人(1987)分离出来了。将含有该基因启动子的EcoRⅠ片段克隆进mp18，该启动子的3′部分直接处于该载体的多聚接头(linker)的上游。然后，用BstⅪ线性化并用Bal31核酸外切酶处理该克隆。先用SalⅠ，然后用Klenow聚合酶处理由此所获得的片段的混合物，最后，在低的DNA浓度下进行连接。对经过该操作而得到的克隆测序，选择具有位于所推定的转录启始位点下游的下列顺序的一种：
…
…5′ATTGGATTC3′…
将ClaⅠ接头(ATCGAT)引入该克隆的PstⅠ位点。这样，通过用Klenow聚合酶处理该CalⅠ位点，并将其与位于该启动子下游的片段上的用Klenow聚合酶处理过的EcoRⅠ位点相连接，重新形成了EcoRⅠ位点，并由此得到从所推定的转录启始位点起呈下列形式的顺序：
ATTGGATTCTCGACCATCGAATTC
                   EcoRⅠ
本发明的另一个特点是，嵌合基因含有位于编码基因和启动子之间的非转译的中间区(接头)，它可从包含下列接头的组中选择：
-一方面，pUC19的接头，通过克隆而修饰，并具有下列顺序：
GAATTCGAGCTCGGTACCCCATGG
EcoRⅠ             NcoⅠ
-另一方面，玉米Rubis CO的小亚基的非转译区，该区来源于由Lebrun等人(1987)所描述的与该基因相应的cDNA。它是一种具有下列顺序的EcoRⅠ-NcoⅠ片段：
GAATTCCAGCAAGCAAGCAGCGAGT
EcoRⅠACATACATACTAGGCAGCCAGGCAGCCATGNcoⅠ
-另一方面，向日葵Rubis CO的小亚基的非转译区：该区来自于由Waksman和Freyssinet(1987)所分离的cDNA。该区尚未如此分离过，它总是被发现在向日葵Rubis CO的转移肽的前面。该顺序如下：
GAATTCCGAAAGACAAAGATTATCG
EcoRⅠ
TAATG
Met
本发明的嵌合基因选择性地包含一个多聚腺苷化区或位点，它们可以是，例如：
1)pTi37(Bevan等人，1983)的蓝曙红合成酶基因的多聚腺苷化位点。该位点包含于260bp的MboⅠ片段(Fraley等人，1983,PCT专利中请，No,84/02913)中，为了将BamHⅠ和EcoRⅠ位点分别引入5′和3′端，该片段已用Klenow聚合酶处理，并克隆入M13mp18的SmaⅠ位点。由Vigna radila核酸酶处理BamHⅠ位点，并克隆到pUC19的用Klenow聚合酶处理过的SalⅠ位点。现在，该片段在其5′末端含有HindⅢ位点，它可以连接到腈水解酶基因的3′末端。
2)玉米Rubis CO的小亚基的基因的多聚腺苷化位点：该位点被分离成如Lebrun等人(1987)所述基因的540个bp的SmaⅠ-BglⅡ片段的形式。将一个ClaⅠ接头(ATCGAT)引入SmaⅠ位点。在用ClaⅠ酶切和用Klenow聚合酶填补后，将该片段克隆进用PstⅠ酶切并用噬菌体T4聚合酶处理及BamHⅠ再酶切的pUC19中。该操作可将HindⅢ位点引入至多聚腺苷化位点的5′末端。所得到的顺序如下：
5′AAGCTTGCATGCCCGATGGGCAG…
HindⅢ              1/SmaⅠ
本发明的又一个特点是，嵌合基因可以选择性地和较佳地包含在中间区和腈水解酶基因之间的转移肽编码区，该编码区可选自包含玉米Rubis CO的小亚基和向日葵小亚基的转移肽编码区的组中。在天然的基因中转移肽的作用是可使Rubis CO小亚基进入叶绿体的基质中。在它们被引入上述中间区和腈水解酶的结构基因之间的情况下，它们也应当同样地将腈水解酶直接引入该基质中：
1)玉米Rubis CO小亚基的转移肽：该片段来自与Lebrun等人(1987)所描述的基因相应的cDNA。它是141个bp的NcoⅠ-SphⅠ片段，NcoⅠ位点覆盖转译启始密码子和SphⅠ位点即转移肽的切割位点。通过用噬菌体T4聚合酶处理该片段的SphⅠ末端，将其与用Klenow聚合酶处理的腈水解酶基因的NcoⅠ末端相连接，重新构造了一个顺序，它允许在叶绿体的基质中产生未经修饰的腈水解酶。
2)向日葵Rubis CO小亚基的转移肽：该片段来自由Waksman和Freyssinel(1987)所分离出来的cDNA。该顺序最初在转移肽的切割位点上不具有SphⅠ位点。该顺序在此处是如下形式的：
5′CAATGCATGA*AG3′
通过定向诱变将带星号的A进行AC置换，由此形成了一个SphⅠ位点。为了进行该操作，可采用Zoller和Smith(1984)的方法。将270bp的EcoRⅠ-SalⅠ片段克隆进M13mp19am4。从M13mp19衍生的该载体在基因4，在5327碱基位置上具有琥珀(ambre)突变，在不具有抑制这种类型突变的抑制基因的菌株中不能增殖。纯化这个重组噬菌体的单链形式后，用三种寡核苷酸分别进行杂交。这种磷酸化的寡核苷酸的顺序如下：
        SphⅠ
1:5′GTTCAATGCATGCAGGTGTGGCCAC3′
2:5′AAGAGTCTGTCCATCAC3′
3:5′GTAAAACGACGGCCAGT3′
它们分别产生如下的可能性：
1：在转移肽的切割位点上片段突变，
2：琥珀突变的矫正。
3．诱变片段上游顺序的引物
Klenow聚合酶在四种核苷酸存在下和噬菌体T4连接酶同时作用后，将获得的混合物转化到菌株HB2154中，然后涂布在含菌株HB2151平板(Carter等人，1985)上。为了验证该结构，将所得到的克隆中具有附加的SphⅠ位点的那些克隆进行测序，其中之一被用于形成嵌合基因。在转移肽的切割位点的地方，现在顺序如下：
5′CAATGCATGC
该片段的使用与用与编码玉米Rubis CO小亚基的转移肽的片段的方法相同。
嵌合基因的构建根据图1-4的途径，用上述的元素而进行。这样所形成的不同的基因被安置于1或2种载体中，每一个构建产物都给予一个参考号。这样所产生的不同的载体在下面表1中描述：
                  表1
含有抗溴苯腈的嵌合基因的不同的TDNAs的构建
pRPA-BL名称 载 体 启动子     接头区 玉米 转移肽向日葵 BR-XN  NOS多聚脾苷酸 玉米SSH多聚腺普酸
CaMV35S  向日葵SSU   玉米5′  接头  向日葵5′
230 150A 1  +   +  +   +
204 150A 1  +   +   -   +  +   +
207 150A 1   +   +  +   +
208 150A 1   +   +   +  +   +
217 150A 1  +   +  +   +
218 150A 1  +   +   +  +   +
221 150A 1   +   +  +   +
222 150A 1   +  +   +
235 142  +   +  +   +
236 142  +   +  +   +
237 142   +   +  +   +
230 142   +   +  +   +
240 142  +   +  +    +
250 142  +  +  +    +
251 142   +   +  +    +
252 142   +  +  +    +
447 142   +   +   +  +    +
所用的载体：
产生显示于图1-4中的载体pRPA-BL-142和pRPA-BL-150A α1的不同的嵌合结构通过Agrobaclerium lumefacients转移系统而引入植物中。为此目的而构造的转移载体具有下列的性质：
-从pBR322衍生的复制和转移起点，
-用于细菌筛选的基因，如对庆大霉素的抗性基因，
-从λ-噬菌体中衍生的一个COS位点，
-pTi A6 T DNA的左右边缘，
-选择性地，诸如抗卡那霉素的真核选择基因，
-选择性地，含有pUC18的lac α互补基因的片段。pRPA-BL-142的构建(图1-4)：
首先，将pTi A6的左T DNA的右边缘和左边缘(图1)进行克隆：
-右边缘：
将在Barker等人(1983)的计数系统中从13774延伸到16202的BamHⅠ-EcoRⅠ片段克隆进pGEM1(Promega Biolech)中相应的位点，得到pBL-17。用NruⅠ(14276和14475)和EcoRⅠ(16202)酶切该质粒，并用Klemow聚合酶处理，将NruⅠ位点与补平的EcoRⅠ位点连接，在14276处重新产生了EcoRⅠ位点，得到质粒pBL-19。
-左边缘
将在Barke等人(1983)的系统中从602延伸到3390的HindⅢ片段克隆到pGEM1中相应的位点，得到pBL-21，其中，左边缘是在多聚接头相对的一边。用AccⅠ(1161-2687)消化该质粒，并在连接前用Klenow聚合酶处理。所得到的质粒pBL-26含有插入于HindⅢ和XbaⅠ位点之间的从602延伸到1161的片段。
TDNA的形成：
通过将pBL-19的EcoRⅠ-BamHⅠ片段插入pBL-26中相应的位点，则重新形成了具有pTiA6的右边缘和左边缘(以它们的天然的方向)的TDNA。所得到的质粒命名为pBL-70。
将TDNA引入pBR322(图2)：
用HindⅢ酶切pBL-70后，用Klenow聚合酶处理该位点，并用EcoRⅠ再酶切该质粒。将所得到的片段克隆进用Pru-Ⅱ-EcoRⅠ酶切的pBR322。所得到的克隆命名为pRPA-BL-112。抗庆大霉素的基因的克隆(图3)：
从pPH1 J1Ⅰ(Hirsh和Bringer,1984)中得到抗庆大霉素的基因。用BamHⅠ和HindⅢ消化该质粒，将收集的片段克隆进用同样的酶切的pUC19。在氨苄青霉素+庆大霉素上选择后，分离出几个含有2.45kbp(千碱基对)片断的克隆。选出来进行随后的操作的克隆被命名为pRPA-BL-133。在该克隆的BamHⅠ位点，插入从pHC79(Hohn和Collins,1980)中分离出来并含有入-噬菌体的COS位点的1.6kbpBglⅡ片段。将该片段双向插入，就能获得pRPA-BL-134和pRPA-BL-135两个克隆。综合载体的制备(图3)：
为了将上述不同的部分结合在一个且是共同的载体上，用SmaⅠ和HindⅢ消化pRPA-BL-134和pRPA-BL-135，并用Klenow聚合酶处理含有抗庆大霉素的基因和λ-噬菌体的COS位点的插入部分。用PstⅠ和EcoRⅠ消化质粒pRPA-BL-112并用噬菌体T4聚合酶处理。将两个片段连接起来，选择同时具有抗庆大霉素，COS位点，TDNA和pBR322复制起点的克隆。质粒pRPA-BL-134产生出pRPA-BL-141和pRPA-BL-142，而pRPA-BL-135则产生出pRPA-143和pRPA-144。pRPA-BL-142被选出以将欲转移的嵌合基因导入植物中。由这个载体，就得到了含有标记基因NOS-NPTⅡ-NOS(图4)的结构。用XbaⅠ消化质粒pRPA-BL-142，并通过Vignaradiata核酸酶的作用处理其末端。而pEND4K(Klee等人，1985)则用EcoRⅠ消化并用Klenow聚合酶处理。分离含有卡那霉素的嵌合基因的1,6kbp的片段，并将其插入pRPA-BL-142。这种连接产物被命名为pRPA-BL-150A，它被选出进行随后的操作。为了便于克隆该载体，含有分离自pUC18(Yannish-Perron等人，1985)的lacα-互补基因的并用噬菌体T4聚合酶处理过的HacⅡ片段被插入用Vigna radiata核酸酶处理过的BamHⅠ位点。所获得的二种载体被命名为pRPA-BL-150Aα1和pRPA-BL-150Aα2。pRPA-BL-150Aα1是用作为将基因导入植物的基础载体。
p-RPA-BL-142和pRPA-BL-150Aα1的应用
这些载体本身在土壤杆菌属(Agrobacterium)中不能维持。为了达到维持的目的，必须将它们通过简单的重组而整合于在该细菌中的质粒中。这可以通过一利片段(如位于cosmids，如pVK102等上)，或用于具有这些顺序的pBR322的片段而进行。对于菌株GV3850(Zambryski等人，1983)的Ti质粒来说就是这种情况，该质粒也是pRPA-BL-142和pRPA-BL-150Aα1的宿主。使用pBR322的复制起点，就可通过Ditte等人(1980)所描述的三部系统将这些质粒转移到土壤杆菌属(Agrobacterium)中。植物材料的转化
为了测试这些嵌合基因的功效，后者根据下面所描述的过程而转移到植物中：
A-转化过程
1．烟草
将载体导入携带cosmidpTVK291(Komari等人，1986)的土壤杆菌EHA101(Hood等人，1987)的非致癌基因菌株中。转化技术是采用Horsh等人(1985)的过程。下面将描述再生工业烟草PBD6(来源：SETA，法国)的过程。
从人工培养叶中再生烟草PBD6是在含有30克蔗糖/升的Murashige和Skoog(MS)基础培养基中进行的。人工培养叶是从栽培于暖房或离体培养的植物中得到的，转化是根据三个连续阶段的叶盘技术(Science1985,V01,227,1229-1231页)进行的。
第一阶段包括在含有0.05mgNAA和2mg/lBAP的MS+30克蔗糖的培养基上15天的苗的诱导。
第二阶段要使在第一阶段中所长成的苗继续长大；它是在不含任何激素的MS+30克庶糖/升的培养基中进行10天。
第三阶段要使苗逐个地移出，并长根。长根培养基含有盐、维生素和糖，稀释二倍。它不含激素。10-15天后，将植入的苗移到土壤中。激素平衡的测定
通过测试25种激素平衡BAP0.2-0.5-1-1.5-2和NAA-0.5-0.05，观察到1.5和2mg/l的BAP和0.05和0.1mg/l的NAA，可获得最佳的再生频率。采用0.05NAA和2mg/lBAP的混合物。
2．其它的双子叶植物
采用携带与用表2所列的植物材料相应载体相关的cosmidpTVK291的土壤杆菌A281的致癌基因菌株转化表2中所列出的双子叶植物。B-抗溴苯腈性的测定
1．烟草
抗性的离体测定是在以辛盐形式存在的溴苯腈(20mg/l)的存在下，通过测定愈伤组织的生长而进行的，在体内则是以10倍于大田处理时所用的剂量的溴苯腈或碘苯腈喷洒叶子而进行的。所得到的结果概括于表1a中。
2．其它双子叶植物
抗性的离体测定是在每升培养基10mg溴苯腈(以辛盐的形式)存在下，测定愈伤组织的生长而进行的。在所有的情况下，结果均呈阳性(表2)。
                   表1a
        经转化的烟草对于溴苯腈的抗性
名称           烟草的抗性pRPA-BL       胼胝体           植物
203            +               +
204            +               +
207            +               +
208            +               +
217            +               +
218            +               +
221            +               +
222            +               +
235            +               +
236            +               +
237            +               +
238            +               +
249            +               +
250            +               +
251            +               +
252            +               +
             表2
  各种双子叶植物对于溴苯腈的抗性
          植物材料      pRPA-BL植物                   203   235    249食用番茄植物    胚轴    +     +      +茄属            块茎    +     +      +大豆属          胚轴    +     +      +甜菜            根      +     +      +向日葵属    胚轴      +    +    +琬豆属      结间部    +    +    +油菜        胚轴      +    +    +胡萝卜      根        +    +    +菜豆        胚轴      +         +

Claims (7)

1．一种转化植物从而赋于其抗溴苯腈和/或碘苯腈除草剂性能的方法，其特征在于，借助于嵌合基因构建物将嵌合基因引入植物细胞，其中，该嵌合基因含有至少一种编码抗溴苯腈和/或碘苯腈除草剂的基因，一个外源启动子和一个可用可不用的多聚腺苷酸化信号区，其中启动子来源于天然地在植物细胞中表达的基因，而且选自花椰菜镶嵌病毒的35S RNA启动子(CamV35S)和向日葵(He-lianthus annuus)核酮糖-1,5-二磷酸羧化酶/加氧酶(RuBisCO)的小亚基(SSU)的启动子。
2．如权利要求1所述的方法，其特征在于嵌合基因包括位于启动子和编码抗除草剂的基因之间的非转译中间区(接头)，所说的非转译中间区选自：通过克隆而修饰的pUC19的非转译区，玉米RuBisCO的小亚基的非转译区和向日葵RuBisCO的小亚基的非转译区。
3．如权利要求1或2所述的方法，其特征在于嵌合基因还含有位于中间区和抗除草剂的编码区之间的编码一个能够将蛋白质引入欲转化的植物的叶绿体中的转移肽的编码区。
4．如权利要求3所述的方法，其特征在于所述的转移肽编码区是玉米RuBisCO的小亚基的转移肽编码区。
5．如权利要求3所述的方法，其特征在于所述的转移肽编码区是向日葵RuBisCO的小亚基的转移肽编码区。
6．如权利要求1所述的方法，其特征在于所述的多聚腺苷酸化区来自于蓝曙红合成酶基因。
7．如权利要求1所述的方法，其特征在于所述的多聚腺苷酸化区来自于玉米RuBisCO的小亚基的基因。
CN89101778A 1988-03-23 1989-03-23 抗除草剂的嵌合基因 Expired - Fee Related CN1046763C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8804130A FR2629098B1 (fr) 1988-03-23 1988-03-23 Gene chimerique de resistance herbicide
FR8804130 1988-03-23

Publications (2)

Publication Number Publication Date
CN1036038A CN1036038A (zh) 1989-10-04
CN1046763C true CN1046763C (zh) 1999-11-24

Family

ID=9364755

Family Applications (1)

Application Number Title Priority Date Filing Date
CN89101778A Expired - Fee Related CN1046763C (zh) 1988-03-23 1989-03-23 抗除草剂的嵌合基因

Country Status (26)

Country Link
US (2) US5559024A (zh)
EP (2) EP0337899B1 (zh)
JP (1) JPH029374A (zh)
KR (2) KR0145703B1 (zh)
CN (1) CN1046763C (zh)
AT (1) ATE188250T1 (zh)
AU (1) AU629539B2 (zh)
BR (1) BR8901499A (zh)
CA (1) CA1314505C (zh)
DD (1) DD294040A5 (zh)
DE (1) DE68929125T2 (zh)
DK (1) DK144689A (zh)
EG (1) EG20026A (zh)
ES (1) ES2139565T3 (zh)
FR (1) FR2629098B1 (zh)
GR (1) GR3032288T3 (zh)
HU (1) HU213581B (zh)
IE (1) IE20001005A1 (zh)
IL (1) IL89614A (zh)
NZ (1) NZ228458A (zh)
PH (1) PH31569A (zh)
PL (1) PL161444B1 (zh)
RO (1) RO107001B1 (zh)
RU (1) RU2106410C1 (zh)
TR (1) TR23682A (zh)
ZA (1) ZA892146B (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101657090B (zh) * 2006-12-12 2015-04-15 巴斯夫农业化学产品公司 抗除草剂的向日葵植物和使用方法

Families Citing this family (241)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2673643B1 (fr) 1991-03-05 1993-05-21 Rhone Poulenc Agrochimie Peptide de transit pour l'insertion d'un gene etranger dans un gene vegetal et plantes transformees en utilisant ce peptide.
USRE36449E (en) * 1991-03-05 1999-12-14 Rhone-Poulenc Agro Chimeric gene for the transformation of plants
US5866379A (en) * 1997-01-28 1999-02-02 Novus International Enzymatic conversion of α-hydroxynitriles to the corresponding .alpha.
US7161064B2 (en) * 1997-08-12 2007-01-09 North Carolina State University Method for producing stably transformed duckweed using microprojectile bombardment
FR2771104B1 (fr) * 1997-11-17 2000-12-08 Rhone Poulenc Agrochimie Gene chimere ayant un promoteur lumiere dependant conferant la tolerance aux inhibiteurs del'hppd
JP2002520024A (ja) * 1998-07-10 2002-07-09 カルジーン エルエルシー 植物色素体における除草剤耐性遺伝子の発現
US6492578B1 (en) * 1998-07-10 2002-12-10 Calgene Llc Expression of herbicide tolerance genes in plant plastids
WO2000012732A2 (en) * 1998-08-28 2000-03-09 Pioneer Hi-Bred International, Inc. Organelle targeting sequences
CA2839066A1 (en) 1999-12-16 2001-06-21 Monsanto Technology Llc Hybrid promoter for use in plants
FR2815969B1 (fr) 2000-10-30 2004-12-10 Aventis Cropscience Sa Plantes tolerantes aux herbicides par contournement de voie metabolique
EP1362113B1 (en) * 2001-02-22 2011-01-12 Biogemma Constitutive promoter from arabidopsis
EP1256629A1 (en) 2001-05-11 2002-11-13 Société des Produits Nestlé S.A. Leaf specifc gene promoter of coffee
CN104206402B (zh) 2007-04-12 2018-04-24 巴斯夫欧洲公司 包含氰基亚磺酰亚胺基化合物的农药混合物
JP2010539213A (ja) 2007-09-20 2010-12-16 ビーエーエスエフ ソシエタス・ヨーロピア 殺菌性株及び活性成分を含む組み合わせ
JP2010540495A (ja) 2007-09-26 2010-12-24 ビーエーエスエフ ソシエタス・ヨーロピア ボスカリド及びクロロタロニルを含む三成分殺菌組成物
CA2724908C (en) * 2008-07-23 2017-03-14 Boehringer Ingelheim Pharma Gmbh & Co. Kg Hgh polyadenylation signal
EP2315760B1 (de) 2008-07-29 2013-03-06 Basf Se Piperazinverbindungen mit herbizider wirkung
EP2183969A3 (en) 2008-10-29 2011-01-05 Basf Se Method for increasing the number of seedlings per number of sowed grains of seed
US20110183848A1 (en) 2008-10-02 2011-07-28 Basf Se Piperazine Compounds With Herbicidal Effect
ES2524819T3 (es) 2009-01-27 2014-12-12 Basf Se Procedimiento de tratamiento de semillas
WO2010089244A1 (en) 2009-02-03 2010-08-12 Basf Se Method for dressing seeds
CN102307478A (zh) 2009-02-11 2012-01-04 巴斯夫欧洲公司 农药混合物
WO2010092014A2 (en) 2009-02-11 2010-08-19 Basf Se Pesticidal mixtures
WO2010092031A2 (en) 2009-02-11 2010-08-19 Basf Se Pesticidal mixtures
BRPI1005355A2 (pt) 2009-02-11 2016-02-10 Basf Se misturas, composição pesticida, método para o controle de pragas e/ou para aprimorar a saúde de plantas, método para a proteção do material de propagação de planta contra pragas e material de propagação de planta
AR075573A1 (es) 2009-02-11 2011-04-20 Basf Se Dimethomorph como protector de plaguicidas con efectos fitotoxicos
AU2010220293B2 (en) 2009-03-04 2014-09-11 Basf Se 3-arylquinazolin-4-one compounds for combating invertebrate pests
WO2010103065A1 (en) 2009-03-11 2010-09-16 Basf Se Fungicidal compositions and their use
WO2010106008A2 (en) 2009-03-16 2010-09-23 Basf Se Fungicidal compositions comprising fluopyram and metrafenone
KR20120014241A (ko) 2009-03-20 2012-02-16 바스프 에스이 캡슐화된 농약으로 작물을 처리하는 방법
NZ594887A (en) 2009-03-26 2013-11-29 Basf Se Use of synthetic and biological fungicides in combination for controlling harmful fungi
CN102369199A (zh) 2009-04-01 2012-03-07 巴斯夫欧洲公司 用于防治无脊椎动物害虫的异*唑啉化合物
US9232785B2 (en) 2009-04-02 2016-01-12 Basf Se Method for reducing sunburn damage in plants
US20120077676A1 (en) 2009-06-12 2012-03-29 Basf Se Antifungal 1,2,4-Triazolyl Derivatives Having a 5-Sulfur Substituent
WO2019106641A2 (en) 2017-12-03 2019-06-06 Seedx Technologies Inc. Systems and methods for sorting of seeds
EP2443098A1 (en) 2009-06-18 2012-04-25 Basf Se Antifungal 1, 2, 4-triazolyl derivatives
WO2010146115A1 (en) 2009-06-18 2010-12-23 Basf Se Triazole compounds carrying a sulfur substituent
EP2442653A2 (de) 2009-06-18 2012-04-25 Basf Se Fungizide mischungen
WO2010146116A1 (en) 2009-06-18 2010-12-23 Basf Se Triazole compounds carrying a sulfur substituent
EP2443097A1 (en) 2009-06-18 2012-04-25 Basf Se Antifungal 1, 2, 4-triazolyl derivatives
EP2443099A1 (en) 2009-06-18 2012-04-25 Basf Se Antifungal 1, 2, 4-triazolyl derivatives having a 5- sulfur substituent
KR20120062679A (ko) 2009-06-18 2012-06-14 바스프 에스이 황 치환기를 보유하는 트리아졸 화합물
SI2443102T1 (sl) 2009-06-19 2013-08-30 Basf Se Herbicidni benzoksazinoni
WO2010149758A1 (en) 2009-06-25 2010-12-29 Basf Se Antifungal 1, 2, 4-triazolyl derivatives
EP2451804B1 (en) 2009-07-06 2014-04-30 Basf Se Pyridazine compounds for controlling invertebrate pests
WO2011003775A2 (de) 2009-07-09 2011-01-13 Basf Se Substituierte cyanobutyrate mit herbizider wirkung
WO2011003776A2 (de) 2009-07-09 2011-01-13 Basf Se Substituierte cyanobutyrate mit herbizider wirkung
BR112012001001A2 (pt) 2009-07-14 2016-11-16 Basf Se compositos azol das formulas i e ii, compostos das formulas i e i, compostos de formula ix, composição agricola, uso de um composto farmaceutica, metodo para tratar infecções de câncer ou virus para combater fungos zoopatigênicos ou humanopatogenicos
WO2011009804A2 (en) 2009-07-24 2011-01-27 Basf Se Pyridine derivatives compounds for controlling invertebrate pests
US20120129696A1 (en) 2009-07-28 2012-05-24 Basf Se Method for increasing the level of free amino acids in storage tissues of perennial plants
MX2012000421A (es) 2009-07-28 2012-02-08 Basf Se Composiciones plaguicidas de suspo - emulsiones.
WO2011014660A1 (en) 2009-07-30 2011-02-03 Merial Limited Insecticidal 4-amino-thieno[2,3-d]-pyrimidine compounds and methods of their use
UY32838A (es) 2009-08-14 2011-01-31 Basf Se "composición activa herbicida que comprende benzoxazinonas
JP2013505909A (ja) 2009-09-24 2013-02-21 ビーエーエスエフ ソシエタス・ヨーロピア 無脊椎動物害虫を駆除するためのアミノキナゾリン化合物
US20120178625A1 (en) 2009-09-25 2012-07-12 Basf Se Method for reducing pistillate flower abortion in plants
AU2010303120A1 (en) 2009-09-29 2012-04-19 Basf Se Pesticidal mixtures
WO2011042378A1 (de) 2009-10-09 2011-04-14 Basf Se Substituierte cyanobutyrate mit herbizider wirkung
DE102010042867A1 (de) 2009-10-28 2011-06-01 Basf Se Verwendung heterozyklischer Verbindungen als Herbizide
WO2011051212A1 (de) 2009-10-28 2011-05-05 Basf Se Verwendung heteroaromatischer verbindungen als herbizide
DE102010042864A1 (de) 2009-10-30 2011-06-01 Basf Se Substituierte Thioamide mit herbizider Wirkung
WO2011051393A1 (en) 2009-11-02 2011-05-05 Basf Se Herbicidal tetrahydrophthalimides
US8329619B2 (en) 2009-11-03 2012-12-11 Basf Se Substituted quinolinones having herbicidal action
WO2011057989A1 (en) 2009-11-11 2011-05-19 Basf Se Heterocyclic compounds having herbicidal action
WO2011057942A1 (en) 2009-11-12 2011-05-19 Basf Se Insecticidal methods using pyridine compounds
WO2011057935A1 (en) 2009-11-13 2011-05-19 Basf Se 3-(3,4-dihydro-2h-benzo [1,4]oxazin-6-yl)-1h-pyrimidin-2,4-dione compounds as herbicides
WO2011058036A1 (en) 2009-11-13 2011-05-19 Basf Se Tricyclic compounds having herbicidal action
US9023874B2 (en) 2009-11-17 2015-05-05 Merial, Inc. Fluorinated oxa or thia heteroarylalkylsulfide derivatives for combating invertebrate pests
EP2504442B1 (en) 2009-11-24 2014-07-16 Katholieke Universiteit Leuven, K.U. Leuven R&D Banana promoters
WO2011064188A1 (en) 2009-11-27 2011-06-03 Basf Se Insecticidal methods using nitrogen-containing heteroaromatic compounds
WO2011067184A1 (de) 2009-12-01 2011-06-09 Basf Se 3- (4, 5 -dihydroisoxazol- 5 -yl) benzoylpyrazolverbindungen und ihre mischungen mit safenern
ES2546100T3 (es) 2009-12-04 2015-09-18 Merial, Inc. Compuestos pesticidas bis-organosulfurados
WO2011069912A1 (de) 2009-12-07 2011-06-16 Basf Se Triazolverbindungen, ihre verwendung sowie sie enthaltende mittel
WO2011069955A1 (en) 2009-12-07 2011-06-16 Basf Se Sulfonimidamide compounds for combating animal pests
WO2011069916A1 (de) 2009-12-08 2011-06-16 Basf Se Triazolverbindungen, ihre verwendung als fungizide sowie sie enthaltende mittel
WO2011069894A1 (de) 2009-12-08 2011-06-16 Basf Se Triazolverbindungen, ihre verwendung sowie sie enthaltende mittel
US20120291159A1 (en) 2009-12-18 2012-11-15 Basf Se Azoline Compounds for Combating Invertebrate Pests
WO2011073143A1 (en) 2009-12-18 2011-06-23 Basf Se Substituted cyanobutyrates having herbicidal action
CN102711456B (zh) 2010-01-18 2015-05-13 巴斯夫欧洲公司 包含农药和2-丙基庚胺的烷氧基化物的组合物
JP2013518084A (ja) 2010-02-01 2013-05-20 ビーエーエスエフ ソシエタス・ヨーロピア 有害動物を駆除するための置換されたケトン性イソオキサゾリン化合物および誘導体
WO2011098417A1 (en) 2010-02-10 2011-08-18 Basf Se Substituted cyanobutyrates having herbicidal action
WO2011101303A2 (de) 2010-02-16 2011-08-25 Basf Se Zusammensetzung umfassend ein pestizid und ein alkoxylat von iso-heptadecylamin
WO2011110583A2 (en) 2010-03-10 2011-09-15 Basf Se Fungicidal mixtures comprising triazole derivatives
WO2011113786A2 (de) 2010-03-17 2011-09-22 Basf Se Zusammensetzung umfassend ein pestizid und ein alkoxylat von verzweigtem nonylamin
CN102834391A (zh) 2010-03-23 2012-12-19 巴斯夫欧洲公司 防治无脊椎动物害虫的哒嗪化合物
JP2013522335A (ja) 2010-03-23 2013-06-13 ビーエーエスエフ ソシエタス・ヨーロピア 除草活性を有するピラジノチアジン
CN102858780A (zh) 2010-03-23 2013-01-02 巴斯夫欧洲公司 具有除草作用的取代哒嗪
AR081526A1 (es) 2010-03-23 2012-10-03 Basf Se Piridazinas sustituidas que tienen accion herbicida
BR112012023757B1 (pt) 2010-03-23 2020-10-20 Basf Se composto de piridazina, método para controlar pragas invertebradas e método para proteger material de propagação de plantas
EP2550271A1 (en) 2010-03-23 2013-01-30 Basf Se Substituted pyridines having herbicidal action
EP2550264B1 (en) 2010-03-23 2016-06-08 Basf Se Pyridazine compounds for controlling invertebrate pests
CN102822178B (zh) 2010-03-23 2015-10-21 巴斯夫欧洲公司 具有除草作用的吡啶并噻嗪
JP2013522339A (ja) 2010-03-23 2013-06-13 ビーエーエスエフ ソシエタス・ヨーロピア 除草作用を有する置換されたピリジン
JP2013529074A (ja) 2010-05-04 2013-07-18 ビーエーエスエフ ソシエタス・ヨーロピア 除草剤に対する耐性が増加した植物
CN103025161A (zh) 2010-05-24 2013-04-03 明治制果药业株式会社 有害生物防治剂
TWI584733B (zh) 2010-05-28 2017-06-01 巴地斯顏料化工廠 包含阿巴汀之農藥混合物、其用途及使用該混合物之方法
TWI501727B (zh) 2010-05-28 2015-10-01 Basf Se 農藥混合物
WO2012007426A1 (en) 2010-07-13 2012-01-19 Basf Se Azoline substituted isoxazoline benzamide compounds for combating animal pests
DE102011080568A1 (de) 2010-08-16 2012-02-16 Basf Se Substituierte Cyanobutyrate mit herbizider Wirkung
BR112013005869A2 (pt) 2010-09-13 2019-09-24 Basf Se ''método para controlar pragas invertebradas, uso de um composto, método, material de propagação vegetal e composição agrícola''
WO2012034960A1 (en) 2010-09-13 2012-03-22 Basf Se Pyridine compounds for controlling invertebrate pests ii
BR112013005382B1 (pt) 2010-09-13 2020-02-18 Basf Se Uso de um composto 3-piridila, método para controlar pragas invertebradas, método para proteger o material de propagação vegetal e/ou plantas e composição agrícola
AU2011303970B2 (en) 2010-09-14 2015-08-13 Basf Se Composition containing a pyripyropene insecticide and an adjuvant
CN103118537B (zh) 2010-09-14 2015-08-12 巴斯夫欧洲公司 含有pyripyropene杀虫剂和碱的农药组合物
CN103228627A (zh) 2010-10-01 2013-07-31 巴斯夫欧洲公司 作为杀虫剂的亚胺取代的2,4-二芳基吡咯啉衍生物
US20130184320A1 (en) 2010-10-01 2013-07-18 Basf Se Imine Compounds
JP2013540113A (ja) 2010-10-01 2013-10-31 ビーエーエスエフ ソシエタス・ヨーロピア 除草性ベンゾオキサジノン
WO2012076704A2 (en) 2010-12-10 2012-06-14 Basf Se Pyrazole compounds for controlling invertebrate pests
CN103261424B (zh) 2010-12-16 2017-12-12 巴斯夫农业公司 对除草剂具有增强的耐受性的植物
AU2011347752A1 (en) 2010-12-20 2013-07-11 Basf Se Pesticidal active mixtures comprising pyrazole compounds
WO2012085081A1 (en) 2010-12-22 2012-06-28 Basf Se Sulfoximinamide compounds for combating invertebrate pests ii
EP2481284A3 (en) 2011-01-27 2012-10-17 Basf Se Pesticidal mixtures
EA022869B1 (ru) 2011-02-28 2016-03-31 Басф Се Композиция, содержащая пестицид, сурфактант и алкоксилат 2-пропилгептиламина
PL2688405T3 (pl) 2011-03-23 2018-05-30 Basf Se Kompozycje zawierające polimerowe, jonowe związki zawierające grupy imidazoliowe
US9179680B2 (en) 2011-04-06 2015-11-10 Basf Se Substituted pyrimidinium compounds for combating animal pests
AR085872A1 (es) 2011-04-08 2013-10-30 Basf Se Derivados heterobiciclicos n-sustituidos utiles para combatir parasitos en plantas y/o animales, composiciones que los contienen y metodos para combatir dichas plagas
WO2013010946A2 (en) 2011-07-15 2013-01-24 Basf Se Pesticidal methods using substituted 3-pyridyl thiazole compounds and derivatives for combating animal pests i
CA2843083A1 (en) 2011-08-12 2013-02-21 Basf Se Anthranilamide compounds and their use as pesticides
JP2014522876A (ja) 2011-08-12 2014-09-08 ビーエーエスエフ ソシエタス・ヨーロピア Ｎ−チオ−アントラニルアミド化合物、及び殺有害生物剤としてのそれらの使用
EP2742021A1 (en) 2011-08-12 2014-06-18 Basf Se Aniline type compounds
IN2014CN01024A (zh) 2011-08-12 2015-04-10 Basf Se
WO2013024003A1 (en) 2011-08-12 2013-02-21 Basf Se N-thio-anthranilamide compounds and their use as pesticides
EP2742037B1 (en) 2011-08-12 2015-10-14 Basf Se N-thio-anthranilamide compounds and their use as pesticides
BR112014002970A2 (pt) 2011-08-12 2017-02-21 Basf Se composto, método para preparar um composto, composição agrícola ou veterinária, método para combater ou controlar pragas invertebradas, método para proteger o cultivo de plantas, método para a proteção de sementes, semente, usos do composto e método para tratar
US20140243196A1 (en) 2011-08-18 2014-08-28 Basf Se Carbamoylmethoxy- and Carbamoylmethylthio- and Carbamoylmethylamino Benzamides for Combating Invertebrate Pests
CN103889960A (zh) 2011-08-18 2014-06-25 巴斯夫欧洲公司 用于防治无脊椎动物害虫的氨基甲酰基甲氧基-和氨基甲酰基甲硫基-及氨基甲酰基甲基氨基苯甲酰胺
JP2014524434A (ja) 2011-08-18 2014-09-22 ビーエーエスエフ ソシエタス・ヨーロピア 有害無脊椎動物を駆除するためのカルバモイルメトキシベンズアミドおよびカルバモイルメチルチオベンズアミドおよびカルバモイルメチルアミノベンズアミド
CN104023535A (zh) 2011-09-02 2014-09-03 巴斯夫欧洲公司 农药活性的3-芳基喹唑啉-4-酮衍生物在土壤施用方法中的用途
JP2014525424A (ja) 2011-09-02 2014-09-29 ビーエーエスエフ ソシエタス・ヨーロピア アリールキナゾリノン化合物を含む農業用混合物
IN2014CN02367A (zh) 2011-09-02 2015-06-19 Basf Se
SI2776038T1 (en) 2011-11-11 2018-06-29 Gilead Apollo, Llc ACC INHIBITORS AND THEIR USE
US20140323306A1 (en) 2011-11-14 2014-10-30 Basf Se Substituted 1,2,5-Oxadiazole Compounds and Their Use as Herbicides
MX2014005607A (es) 2011-11-16 2014-07-30 Basf Se Compuestos de 1,2,5-oxadiazol sustituido y su uso como herbicidas ii.
CN104039780A (zh) 2011-11-18 2014-09-10 巴斯夫欧洲公司 取代的1,2,5-噁二唑化合物及其作为除草剂的用途iii
CN104023724A (zh) 2011-12-21 2014-09-03 巴斯夫欧洲公司 N-硫代邻氨基苯甲酰胺化合物及其作为农药的用途
WO2013113789A1 (en) 2012-02-02 2013-08-08 Basf Se N-thio-anthranilamide compounds and their use as pesticides
BR112014021199A2 (pt) 2012-03-01 2018-05-08 Basf Se composição, método para preparação da composição, polímero, método para preparação do polímero, método para controlar fungos e semente
KR102056608B1 (ko) 2012-03-12 2019-12-17 바스프 에스이 피리피로펜 살곤충제의 수성 현탁액 농축물 제형의 제조 방법
US9596843B2 (en) 2012-03-12 2017-03-21 Basf Se Liquid concentrate formulation containing a pyripyropene insecticide I
MX360700B (es) 2012-03-12 2018-11-14 Basf Se Formulacion concentrada liquida que contiene un insecticida de piripiropeno ii.
US20150031535A1 (en) 2012-03-13 2015-01-29 Basf Se Liquid concentrate formulation containing a pyripyropene insecticide III
WO2013144228A1 (en) 2012-03-29 2013-10-03 Basf Se Pesticidal methods using heterocyclic compounds and derivatives for combating animal pests
WO2013144223A1 (en) 2012-03-30 2013-10-03 Basf Se N-substituted pyrimidinylidene compounds and derivatives for combating animal pests
ES2626360T3 (es) 2012-03-30 2017-07-24 Basf Se Compuestos de piridinilideno tiocarbonilo N-sustituidos y su uso para combatir plagas de animales
WO2013149903A1 (en) 2012-04-03 2013-10-10 Basf Se N- substituted hetero - bicyclic furanone derivatives for combating animal
WO2013150115A1 (en) 2012-04-05 2013-10-10 Basf Se N- substituted hetero - bicyclic compounds and derivatives for combating animal pests
US20150291570A1 (en) 2012-04-27 2015-10-15 Basf Se Substituted N-(tetrazol-5-yl)- and N-(triazol-5-yl)arylcarboxamide compounds and their use as herbicides
JP2015519316A (ja) 2012-04-27 2015-07-09 ビーエーエスエフ ソシエタス・ヨーロピアＢａｓｆ Ｓｅ 置換型ｎ−（テトラゾール−５−イル）−およびｎ−（トリアゾール−５−イル）ヘタリールカルボキサミド化合物ならびに除草剤としてのそれらの使用
IN2014MN02211A (zh) 2012-04-27 2015-07-10 Basf Se
BR112014026789B1 (pt) 2012-04-27 2019-10-22 Basf Se compostos n-(tetrazol-5-il) e n-(triazol-5-il)aril carboxamidas, composição, uso de um composto e método para controlar vegetação indesejada
EA201401213A1 (ru) 2012-05-04 2015-04-30 Басф Се Замещенные пиразолсодержащие соединения и их применение в качестве пестицидов
CN104487439B (zh) 2012-05-24 2017-06-09 巴斯夫欧洲公司 N‑硫代邻氨基苯甲酰胺化合物及其作为杀害虫剂的用途
WO2013186089A2 (en) 2012-06-14 2013-12-19 Basf Se Pesticidal methods using substituted 3-pyridyl thiazole compounds and derivatives for combating animal pests
AR091489A1 (es) 2012-06-19 2015-02-11 Basf Se Plantas que tienen una mayor tolerancia a herbicidas inhibidores de la protoporfirinogeno oxidasa (ppo)
EP3646731A1 (en) 2012-06-20 2020-05-06 Basf Se Pesticidal mixtures comprising a pyrazole compound
WO2013189777A1 (en) 2012-06-21 2013-12-27 Basf Se Adjuvant comprising a 2-propylheptylamine alkoxylate, sugar-based surfactant, and drift-control agent and/or humectant
EP2684879A1 (en) 2012-07-09 2014-01-15 Basf Se Substituted mesoionic compounds for combating animal pests
BR112015006299A2 (pt) 2012-09-21 2017-07-04 Basf Se ''composto, composição agrícola, método para a proteção dos vegetais de cultura, método para a proteção do material de propagação dos vegetais e material de propagação''
CN104768377A (zh) 2012-10-01 2015-07-08 巴斯夫欧洲公司 包含邻氨基苯甲酰胺类化合物的农药活性混合物
WO2014053401A2 (en) 2012-10-01 2014-04-10 Basf Se Method of improving plant health
WO2014053403A1 (en) 2012-10-01 2014-04-10 Basf Se Method of controlling insecticide resistant insects
WO2014053407A1 (en) 2012-10-01 2014-04-10 Basf Se N-thio-anthranilamide compounds and their use as pesticides
AR094139A1 (es) 2012-10-01 2015-07-15 Basf Se Mezclas activas como plaguicidas, que comprenden compuestos de antranilamida
WO2014053406A1 (en) 2012-10-01 2014-04-10 Basf Se Method of controlling ryanodine-modulator insecticide resistant insects
JP2016501264A (ja) 2012-12-14 2016-01-18 ビーエーエスエフ ソシエタス・ヨーロピアＢａｓｆ Ｓｅ 動物有害生物を防除するためのマロノニトリル化合物
JP2016505585A (ja) 2012-12-21 2016-02-25 ビーエーエスエフ ソシエタス・ヨーロピアＢａｓｆ Ｓｅ 無脊椎有害生物を防除するためのシクロクラビン及びその誘導体
WO2014128136A1 (en) 2013-02-20 2014-08-28 Basf Se Anthranilamide compounds and their use as pesticides
US20160050923A1 (en) 2013-04-19 2016-02-25 Basf Se N-substituted acyl-imino-pyridine compounds and derivatives for combating animal pests
CA2911818A1 (en) 2013-05-10 2014-11-13 Nimbus Apollo, Inc. Acc inhibitors and uses thereof
AU2014262638A1 (en) 2013-05-10 2015-11-26 Gilead Apollo, Llc ACC inhibitors and uses thereof
WO2014184058A1 (en) 2013-05-15 2014-11-20 Basf Se Substituted 1,2,5-oxadiazole compounds and their use as herbicides
WO2014184019A1 (en) 2013-05-15 2014-11-20 Basf Se N-(1,2,5-oxadiazol-3-yl)carboxamide compounds and their use as herbicides
BR112015028597A2 (pt) 2013-05-15 2017-07-25 Basf Se n-(tetrazol-5-il)- e n-(5-triazol-il)arilcarboxamidas, composição, utilização de um composto e método para o controle da vegetação
WO2014184014A1 (en) 2013-05-15 2014-11-20 Basf Se N-(1,2,5-oxadiazol-3-yl)carboxamide compounds and their use as herbicides
EP2815649A1 (en) 2013-06-18 2014-12-24 Basf Se Fungicidal mixtures II comprising strobilurin-type fungicides
CN105377836B (zh) 2013-07-18 2018-04-10 巴斯夫欧洲公司 取代的n‑(1,2,4‑三唑‑3‑基)芳基羧酰胺化合物及其作为除草剂的用途
EP2835052A1 (en) 2013-08-07 2015-02-11 Basf Se Fungicidal mixtures comprising pyrimidine fungicides
US10619138B2 (en) 2013-08-12 2020-04-14 Basf Se Herbicide-resistant hydroxyphenylpyruvate dioxygenases
AR097362A1 (es) 2013-08-16 2016-03-09 Cheminova As Combinación de 2-metilbifenil-3-ilmetil (z)-(1r)-cis-3-(2-cloro-3,3,3-trifluorprop-1-enil)-2, 2-dimetilciclopropanocarboxilato con por lo menos un insecticida, acaricida, nematicida y/o fungicida
CA2922506A1 (en) 2013-09-19 2015-03-26 Basf Se N-acylimino heterocyclic compounds
WO2015052178A1 (en) 2013-10-10 2015-04-16 Basf Se 1,2,5-oxadiazole compounds and their use as herbicides
CN105636950A (zh) 2013-10-10 2016-06-01 巴斯夫欧洲公司 取代的n-(四唑-5-基)-和n-(三唑-5-基)芳基羧酰胺化合物及其作为除草剂的用途
WO2015052173A1 (en) 2013-10-10 2015-04-16 Basf Se Tetrazole and triazole compounds and their use as herbicides
WO2015055757A1 (en) 2013-10-18 2015-04-23 Basf Se Use of pesticidal active carboxamide derivative in soil and seed application and treatment methods
EP2868197A1 (en) 2013-11-05 2015-05-06 Basf Se Herbicidal compositions
EP2868196A1 (en) 2013-11-05 2015-05-06 Basf Se Herbicidal compositions
EA201691198A1 (ru) 2013-12-18 2016-11-30 Басф Агро Б.В. Растения, обладающие повышенной толерантностью к воздействию гербицидов
EP3083581A1 (en) 2013-12-18 2016-10-26 Basf Se N-substituted imino heterocyclic compounds
AR100304A1 (es) 2014-02-05 2016-09-28 Basf Corp Formulación de recubrimiento de semillas
EP2907807A1 (en) 2014-02-18 2015-08-19 Basf Se Benzamide compounds and their use as herbicides
WO2015150465A2 (en) 2014-04-03 2015-10-08 Basf Se Plants having increased tolerance to herbicides
RU2704450C2 (ru) 2014-05-06 2019-10-28 Басф Се Композиция, содержащая пестицид и гидроксиалкиловый простой эфир полиоксиленгликоля
EA036537B1 (ru) 2014-06-25 2020-11-20 Басф Агро Б.В. Пестицидные композиции
EP2962567A1 (en) 2014-07-01 2016-01-06 Basf Se Ternary mixtures comprising biopesticides and at least two chemical insecticides
UA120058C2 (uk) 2014-07-14 2019-09-25 Басф Се Пестицидні композиції
WO2016034615A1 (en) 2014-09-02 2016-03-10 BASF Agro B.V. Aqueous insecticide formulation containing hyperbranched polymer
EP3227448A1 (en) 2014-12-01 2017-10-11 Basf Se A method for screening of genes conferring increased tolerance to herbicides
EP3247200A4 (en) 2015-01-21 2018-06-13 Basf Se Plants having increased tolerance to herbicides
US10757935B2 (en) 2015-02-10 2020-09-01 Basf Se Composition comprising a pesticide and an alkoxylated ester
AR103649A1 (es) 2015-02-11 2017-05-24 Basf Se Hidroxifenilpiruvato dioxigenasas resistentes a herbicidas
EP3061346A1 (en) 2015-02-26 2016-08-31 Bayer CropScience AG Use of fluopyram and biological control agents to control harmful fungi
AU2016239537B2 (en) 2015-03-31 2020-02-06 Basf Se Composition comprising a pesticide and isononanoic acid N,N-dimethyl amide
WO2016174042A1 (en) 2015-04-27 2016-11-03 BASF Agro B.V. Pesticidal compositions
US20180312864A1 (en) 2015-10-22 2018-11-01 Basf Se Plants having increased tolerance to herbicides
ES2939977T3 (es) 2015-11-25 2023-04-28 Gilead Apollo Llc Inhibidores de triazol ACC y usos de los mismos
EP3380480B1 (en) 2015-11-25 2022-12-14 Gilead Apollo, LLC Pyrazole acc inhibitors and uses thereof
HUE053175T2 (hu) 2015-11-25 2021-06-28 Gilead Apollo Llc ACC-gátló észterek és azok alkalmazása
CR20180370A (es) 2015-12-17 2018-10-18 Basf Se Compuestos de benzamida y sus usos como herbicidas
UY37137A (es) 2016-02-24 2017-09-29 Merial Inc Compuestos antiparasitarios de isoxazolina, formulaciones inyectables de acción prolongada que los comprenden, métodos y usos de los mismos
US20190098899A1 (en) 2016-03-10 2019-04-04 Basf Se Fungicidal mixtures iii comprising strobilurin-type fungicides
EP3245872A1 (en) 2016-05-20 2017-11-22 BASF Agro B.V. Pesticidal compositions
WO2017207368A1 (en) 2016-06-02 2017-12-07 BASF Agro B.V. Fungicidal compositions
CA3030803A1 (en) 2016-07-15 2018-01-18 Basf Se Plants having increased tolerance to herbicides
BR112019001483A2 (pt) 2016-07-27 2019-09-10 Basf Agro Bv método para controlar a vegetação indesejada em um local de cultivo de plantas
CA3044728A1 (en) 2016-12-20 2018-06-28 BASF Agro B.V. Plants having increased tolerance to herbicides
WO2018141575A1 (en) 2017-02-01 2018-08-09 Basf Se Emulsifiable concentrate
AU2018276360A1 (en) 2017-05-30 2019-12-19 Basf Se Benzamide compounds and their use as herbicides II
EP3630734A1 (en) 2017-05-30 2020-04-08 Basf Se Benzamide compounds and their use as herbicides
AR112112A1 (es) 2017-06-20 2019-09-18 Basf Se Compuestos de benzamida y su uso como herbicidas
AU2018287131B2 (en) 2017-06-23 2023-10-12 Basf Se Pesticidal mixtures comprising a pyrazole compound
CN110891923A (zh) 2017-07-10 2020-03-17 巴斯夫欧洲公司 包含脲酶抑制剂(ui)和硝化抑制剂如2-(3,4-二甲基-1h-吡唑-1-基)琥珀酸(dmpsa)或3,4-二甲基吡唑鎓乙醇酸盐(dmpg)的混合物
WO2019016385A1 (en) 2017-07-21 2019-01-24 Basf Se BENZAMIDE COMPOUNDS AND THEIR USE AS HERBICIDES
CN111246738A (zh) 2017-11-15 2020-06-05 巴斯夫欧洲公司 桶混物
AU2018376915A1 (en) 2017-11-29 2020-05-21 Basf Se Plants having increased tolerance to herbicides
WO2019105995A1 (en) 2017-11-29 2019-06-06 Basf Se Benzamide compounds and their use as herbicides
EP3707640A1 (en) 2017-12-03 2020-09-16 Seedx Technologies Inc. Systems and methods for sorting of seeds
WO2019106638A1 (en) 2017-12-03 2019-06-06 Seedx Technologies Inc. Systems and methods for sorting of seeds
WO2019122347A1 (en) 2017-12-22 2019-06-27 Basf Se N-(1,2,5-oxadiazol-3-yl)-benzamide compounds and their use as herbicides
WO2019122345A1 (en) 2017-12-22 2019-06-27 Basf Se Benzamide compounds and their use as herbicides
EP3508480A1 (en) 2018-01-08 2019-07-10 Basf Se Benzamide compounds and their use as herbicides
BR112020013929A2 (pt) 2018-01-17 2020-12-01 Basf Se plantas ou partes da planta, semente, células vegetais, produto vegetal, progênie ou planta descendente, métodos para controlar ervas daninhas, para produzir uma planta e para produzir uma planta descendente, molécula de ácido nucleico, cassete de expressão, vetor, polipeptídeo, método para produzir um produto vegetal e produto vegetal
WO2019162308A1 (en) 2018-02-21 2019-08-29 Basf Se Benzamide compounds and their use as herbicides
WO2019162309A1 (en) 2018-02-21 2019-08-29 Basf Se Benzamide compounds and their use as herbicides
WO2020058010A1 (en) 2018-09-19 2020-03-26 Basf Se Pesticidal mixtures comprising a mesoionic compound
CA3112042A1 (en) 2018-09-28 2020-04-02 Basf Se Method of controlling pests by seed treatment application of a mesoionic compound or mixture thereof
EP3680223A1 (en) 2019-01-10 2020-07-15 Basf Se Mixture comprising an urease inhibitor (ui) and a nitrification inhibitor (ni) such as an ni mixture comprising 2-(3,4-dimethyl-1h-pyrazol-1-yl)succinic acid (dmpsa) and dicyandiamide (dcd)
CA3162521A1 (en) 2019-12-23 2021-07-01 Basf Se Enzyme enhanced root uptake of agrochemical active compound
US20230063109A1 (en) 2020-01-16 2023-03-02 Basf Se Mixtures comprising nitrification inhibitors and carriers
CA3164114A1 (en) 2020-01-16 2021-07-22 Gregor Pasda Mixtures comprising a solid carrier comprising an urease inhibitor and a further solid carrier comprising a nitrification inhibitor
CN116234444A (zh) 2020-07-06 2023-06-06 皮埃企业有限公司 包含硫杂环丁烷氧基化合物、其氧化物或其盐的具有杀虫活性的混合物
TW202220557A (zh) 2020-07-27 2022-06-01 印度商皮埃企業有限公司 包含吡唑並吡啶鄰氨基苯甲酰胺化合物、其氧化物或鹽的農藥活性混合物
CN117897378A (zh) 2021-09-03 2024-04-16 巴斯夫农业种子解决方案美国有限责任公司 对除草剂具有增加的耐受性的植物

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0131623B2 (en) * 1983-01-17 1999-07-28 Monsanto Company Chimeric genes suitable for expression in plant cells
AU590597B2 (en) * 1985-08-07 1989-11-09 Monsanto Technology Llc Glyphosate-resistant plants
JPS62201527A (ja) * 1985-10-29 1987-09-05 モンサント・カンパニ− ウイルス感染に対する植物保護
KR950008571B1 (ko) * 1986-01-08 1995-08-03 롱쁠랑 아그로시미 할로아릴니트릴 분해 유전자, 그의 용도 및 이를 함유한 세포
US4810648A (en) * 1986-01-08 1989-03-07 Rhone Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the gene
EP0242236B2 (en) * 1986-03-11 1996-08-21 Plant Genetic Systems N.V. Plant cells resistant to glutamine synthetase inhibitors, made by genetic engineering
MA20977A1 (fr) * 1986-05-19 1987-12-31 Ciba Geigy Ag Plantes tolerant les herbicides contenant le gene de gluthathione S-Transferase

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101657090B (zh) * 2006-12-12 2015-04-15 巴斯夫农业化学产品公司 抗除草剂的向日葵植物和使用方法

Also Published As

Publication number Publication date
AU3159189A (en) 1989-09-28
DD294040A5 (de) 1991-09-19
KR890014742A (ko) 1989-10-25
ATE188250T1 (de) 2000-01-15
KR100218855B1 (ko) 1999-10-01
RO107001B1 (ro) 1993-08-30
RU2106410C1 (ru) 1998-03-10
DE68929125D1 (de) 2000-02-03
EP0337899B1 (fr) 1999-12-29
AU629539B2 (en) 1992-10-08
JPH029374A (ja) 1990-01-12
DE68929125T2 (de) 2000-05-31
CA1314505C (en) 1993-03-16
PH31569A (en) 1998-11-03
EP0337899A1 (fr) 1989-10-18
ZA892146B (en) 1989-11-29
DK144689D0 (da) 1989-03-22
PL161444B1 (pl) 1993-06-30
GR3032288T3 (en) 2000-04-27
EP0894865A3 (fr) 1999-04-14
US5559024A (en) 1996-09-24
IL89614A0 (en) 1989-09-10
KR19990030697A (ko) 1999-05-06
FR2629098A1 (fr) 1989-09-29
ES2139565T3 (es) 2000-02-16
US5773698A (en) 1998-06-30
KR0145703B1 (ko) 1998-08-01
EG20026A (fr) 1997-10-30
HU213581B (en) 1997-08-28
PL278448A1 (en) 1989-12-27
FR2629098B1 (fr) 1990-08-10
HUT52158A (en) 1990-06-28
IL89614A (en) 1996-01-19
NZ228458A (en) 1991-06-25
DK144689A (da) 1989-09-24
BR8901499A (pt) 1989-11-14
IE20001005A1 (en) 2001-05-30
CN1036038A (zh) 1989-10-04
TR23682A (tr) 1990-06-06
EP0894865A2 (fr) 1999-02-03

Similar Documents

Publication Publication Date Title
CN1046763C (zh) 抗除草剂的嵌合基因
CN1039133C (zh) 腈水解酶的制法
CN1930293B (zh) 具有降低的饱和脂肪酸水平的转基因植物及其制备方法
CN1452660A (zh) 植物稳定转化的方法
CN109182373B (zh) 一种利用基因编辑技术获得高油酸油菜的方法
ZA200601438B (en) Fatty acid desaturases from primula
IL87001A (en) Nitrilease free of Calabashilla bacteria, a sequence of dents that encodes it and cells that express it
CN103826439A (zh) 植物转化方法
CN1284999A (zh) 与水稻肌动蛋白第一内含子相联的玉米h3c4启动子,含有它的嵌合基因及转化植物
CN87104202A (zh) 植物种的转化和外源基因的表达
US11591580B2 (en) K85 mutation-containing plant EPSPS mutant, and encoding gene and application thereof
CN113106120B (zh) 针叶树植物基因编辑载体、构建方法、及其应用
CN107099544A (zh) 在水稻基因打靶中识别特异位点的PL‑LbCpf1‑RVR基因及其应用
US20030154518A1 (en) Removal of selectable markers from transformed cells
CA3083031A1 (en) Plant epsps mutant containing l195p and s247g mutations and encoding gene and use thereof
NL2027897B1 (en) Use of gmaap protein and gmaap gene in breeding soybeans
CN1296526A (zh) 生产耐受或抗除草剂的植物的方法
AU2015323980B2 (en) Plants with engineered endogenous genes
CN104004777A (zh) 抗草甘膦基因、专用表达载体及其在获得抗草甘膦转基因小麦中的应用
CN114990153A (zh) 水稻脂质转移蛋白在提高稻米脂肪酸含量和降低稻米垩白度中的应用
KR102038481B1 (ko) 레스퀘렐라 유래 PfFAD3-1 유전자로 형질전환된 수확량이 증가된 콩 식물체 및 이의 제조방법
WO1996023072A1 (de) Für glutathion-s-transferase kodierende desoxyribonukleinsäure und ihre verwendung
CN112430613A (zh) 一种宽编辑范围的SpG基因及其应用
WO2011031006A2 (ko) ＣｒｙＩＡｃ 유전자로 형질전환된 배추좀나방 저항성 양배추 및 이의 제조 방법
KR100496028B1 (ko) 제초제 저항성 고추 식물체의 제조방법

Legal Events

Date Code Title Description
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C06 Publication
PB01 Publication
C14 Grant of patent or utility model
GR01 Patent grant
C15 Extension of patent right duration from 15 to 20 years for appl. with date before 31.12.1992 and still valid on 11.12.2001 (patent law change 1993)
OR01 Other related matters
C19 Lapse of patent right due to non-payment of the annual fee
CF01 Termination of patent right due to non-payment of annual fee