CN104232365A - 在宽温度范围内具有活性的蛋白酶变体 - Google Patents

在宽温度范围内具有活性的蛋白酶变体 Download PDF

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Publication number
CN104232365A
CN104232365A CN201410407979.4A CN201410407979A CN104232365A CN 104232365 A CN104232365 A CN 104232365A CN 201410407979 A CN201410407979 A CN 201410407979A CN 104232365 A CN104232365 A CN 104232365A
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China
Prior art keywords
subtilisin
protease
protein
sequence
gene
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Pending
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CN201410407979.4A
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English (en)
Chinese (zh)
Inventor
P·奥古斯丁
F·齐德戈伯
A·J·保罗斯
J·C·范德莱
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Danisco USA Inc
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Danisco USA Inc
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Publication of CN104232365A publication Critical patent/CN104232365A/zh
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38681Chemically modified or immobilised enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201410407979.4A 2006-07-18 2007-07-09 在宽温度范围内具有活性的蛋白酶变体 Pending CN104232365A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US83173206P 2006-07-18 2006-07-18
US60/831,732 2006-07-18

Related Parent Applications (1)

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CNA2007800269622A Division CN101511998A (zh) 2006-07-18 2007-07-09 在宽温度范围内具有活性的蛋白酶变体

Publications (1)

Publication Number Publication Date
CN104232365A true CN104232365A (zh) 2014-12-24

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Family Applications (2)

Application Number Title Priority Date Filing Date
CNA2007800269622A Pending CN101511998A (zh) 2006-07-18 2007-07-09 在宽温度范围内具有活性的蛋白酶变体
CN201410407979.4A Pending CN104232365A (zh) 2006-07-18 2007-07-09 在宽温度范围内具有活性的蛋白酶变体

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CNA2007800269622A Pending CN101511998A (zh) 2006-07-18 2007-07-09 在宽温度范围内具有活性的蛋白酶变体

Country Status (13)

Country Link
US (6) US20080090747A1 (enExample)
EP (1) EP2059591B1 (enExample)
JP (2) JP2009543577A (enExample)
KR (2) KR20090031906A (enExample)
CN (2) CN101511998A (enExample)
AU (1) AU2007275864B2 (enExample)
BR (1) BRPI0714329B1 (enExample)
CA (1) CA2658396C (enExample)
DK (1) DK2059591T3 (enExample)
MX (1) MX2009000537A (enExample)
NZ (1) NZ573467A (enExample)
RU (1) RU2509152C2 (enExample)
WO (1) WO2008010925A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110312795A (zh) * 2016-12-21 2019-10-08 丹尼斯科美国公司 蛋白酶变体及其用途

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