CN101511998A - 在宽温度范围内具有活性的蛋白酶变体 - Google Patents

在宽温度范围内具有活性的蛋白酶变体 Download PDF

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Publication number
CN101511998A
CN101511998A CNA2007800269622A CN200780026962A CN101511998A CN 101511998 A CN101511998 A CN 101511998A CN A2007800269622 A CNA2007800269622 A CN A2007800269622A CN 200780026962 A CN200780026962 A CN 200780026962A CN 101511998 A CN101511998 A CN 101511998A
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China
Prior art keywords
protease
protein
sequence
gene
subtilisin
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Pending
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CNA2007800269622A
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English (en)
Chinese (zh)
Inventor
P·奥古斯丁
F·齐德戈伯
A·J·保罗斯
J·C·范德莱
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Danisco US Inc
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Danisco US Inc
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Publication of CN101511998A publication Critical patent/CN101511998A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38681—Chemically modified or immobilised enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CNA2007800269622A 2006-07-18 2007-07-09 在宽温度范围内具有活性的蛋白酶变体 Pending CN101511998A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US83173206P 2006-07-18 2006-07-18
US60/831,732 2006-07-18

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201410407979.4A Division CN104232365A (zh) 2006-07-18 2007-07-09 在宽温度范围内具有活性的蛋白酶变体

Publications (1)

Publication Number Publication Date
CN101511998A true CN101511998A (zh) 2009-08-19

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ID=38893328

Family Applications (2)

Application Number Title Priority Date Filing Date
CNA2007800269622A Pending CN101511998A (zh) 2006-07-18 2007-07-09 在宽温度范围内具有活性的蛋白酶变体
CN201410407979.4A Pending CN104232365A (zh) 2006-07-18 2007-07-09 在宽温度范围内具有活性的蛋白酶变体

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201410407979.4A Pending CN104232365A (zh) 2006-07-18 2007-07-09 在宽温度范围内具有活性的蛋白酶变体

Country Status (13)

Country Link
US (6) US20080090747A1 (enExample)
EP (1) EP2059591B1 (enExample)
JP (2) JP2009543577A (enExample)
KR (2) KR20150115023A (enExample)
CN (2) CN101511998A (enExample)
AU (1) AU2007275864B2 (enExample)
BR (1) BRPI0714329B1 (enExample)
CA (1) CA2658396C (enExample)
DK (1) DK2059591T3 (enExample)
MX (1) MX2009000537A (enExample)
NZ (1) NZ573467A (enExample)
RU (1) RU2509152C2 (enExample)
WO (1) WO2008010925A2 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113699138A (zh) * 2021-08-19 2021-11-26 邵阳学院 碱性蛋白酶基因、杂合启动子、重组表达载体、重组表达工程菌、碱性蛋白酶及方法和应用

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