CA2900882A1 - Production efficace de glycosides steviol dans les hotes recombinants - Google Patents

Production efficace de glycosides steviol dans les hotes recombinants Download PDF

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Publication number
CA2900882A1
CA2900882A1 CA2900882A CA2900882A CA2900882A1 CA 2900882 A1 CA2900882 A1 CA 2900882A1 CA 2900882 A CA2900882 A CA 2900882A CA 2900882 A CA2900882 A CA 2900882A CA 2900882 A1 CA2900882 A1 CA 2900882A1
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Canada
Prior art keywords
recombinant microorganism
rebaudioside
steviol
gene
genes
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Abandoned
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CA2900882A
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English (en)
Inventor
Ernesto SIMON
Iben Nordmark Andersen
Michael Dalgaard Mikkelsen
Jorgen Hansen
Veronique Douchin
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Evolva Holding SA
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Evolva AG
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Publication date
Application filed by Evolva AG filed Critical Evolva AG
Publication of CA2900882A1 publication Critical patent/CA2900882A1/fr
Abandoned legal-status Critical Current

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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
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    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
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    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
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    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1062—Sucrose synthase (2.4.1.13)
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    • C12N9/10—Transferases (2.)
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    • C12N9/88—Lyases (4.)
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    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12Y106/02004—NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase
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    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13078—Ent-kaurene oxidase (1.14.13.78)
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    • C12Y204/00—Glycosyltransferases (2.4)
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    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01013—Sucrose synthase (2.4.1.13)
    • C—CHEMISTRY; METALLURGY
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    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01035—Phenol beta-glucosyltransferase (2.4.1.35)
    • C—CHEMISTRY; METALLURGY
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    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01029—Geranylgeranyl diphosphate synthase (2.5.1.29)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
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    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03019—Ent-kaurene synthase (4.2.3.19)
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    • C12Y505/00—Intramolecular lyases (5.5)
    • C12Y505/01—Intramolecular lyases (5.5.1)
    • C12Y505/01013—Ent-copalyl diphosphate synthase (5.5.1.13)

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  • Engineering & Computer Science (AREA)
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  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention concerne des microorganismes recombinés qui produisent des glycosides de stéviol et dont l'expression d'un ou plusieurs gènes de transporteurs ou de facteurs de transcription endogènes est modifiée ou qui surexpriment un ou plusieurs transporteurs hétérologues, ce qui permet d'augmenter l'excrétion de glycosides de stéviol d'intérêt.
CA2900882A 2013-02-11 2014-02-11 Production efficace de glycosides steviol dans les hotes recombinants Abandoned CA2900882A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201361763290P 2013-02-11 2013-02-11
US201361763308P 2013-02-11 2013-02-11
US61/763,290 2013-02-11
US61/763,308 2013-02-11
PCT/EP2014/052675 WO2014122328A1 (fr) 2013-02-11 2014-02-11 Production efficace de glycosides de stéviol dans des hôtes recombinés

Publications (1)

Publication Number Publication Date
CA2900882A1 true CA2900882A1 (fr) 2014-08-14

Family

ID=50112893

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2900882A Abandoned CA2900882A1 (fr) 2013-02-11 2014-02-11 Production efficace de glycosides steviol dans les hotes recombinants

Country Status (12)

Country Link
US (3) US10017804B2 (fr)
EP (1) EP2954061B1 (fr)
JP (2) JP6576247B2 (fr)
KR (1) KR20150128705A (fr)
CN (1) CN105189771B (fr)
AU (2) AU2014213918B2 (fr)
BR (1) BR112015019160A2 (fr)
CA (1) CA2900882A1 (fr)
MX (1) MX2015010098A (fr)
MY (1) MY184253A (fr)
SG (2) SG10201705993YA (fr)
WO (1) WO2014122328A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106715451A (zh) * 2014-10-03 2017-05-24 康纳根有限公司 无热量甜味剂及合成方法

Families Citing this family (66)

* Cited by examiner, † Cited by third party
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AU2014213918A1 (en) 2015-08-20
CN105189771A (zh) 2015-12-23
US11021727B2 (en) 2021-06-01
SG11201506127WA (en) 2015-09-29
US20150361476A1 (en) 2015-12-17
CN105189771B (zh) 2019-11-05
EP2954061B1 (fr) 2023-11-22
AU2014213918B2 (en) 2017-11-09
SG10201705993YA (en) 2017-08-30
WO2014122328A8 (fr) 2015-09-17
KR20150128705A (ko) 2015-11-18
US10017804B2 (en) 2018-07-10
JP2019162110A (ja) 2019-09-26
EP2954061A1 (fr) 2015-12-16

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