CA2889660C - Formation of array of membranes and apparatus therefor - Google Patents
Formation of array of membranes and apparatus therefor Download PDFInfo
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- CA2889660C CA2889660C CA2889660A CA2889660A CA2889660C CA 2889660 C CA2889660 C CA 2889660C CA 2889660 A CA2889660 A CA 2889660A CA 2889660 A CA2889660 A CA 2889660A CA 2889660 C CA2889660 C CA 2889660C
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| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| JP5843614B2 (ja) | 2009-01-30 | 2016-01-13 | オックスフォード ナノポア テクノロジーズ リミテッド | 膜貫通配列決定における核酸構築物のためのアダプター |
| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| AU2012264497B2 (en) | 2011-05-27 | 2017-06-15 | Oxford Nanopore Technologies Limited | Coupling method |
| JP6298404B2 (ja) | 2011-07-25 | 2018-03-20 | オックスフォード ナノポール テクノロジーズ リミテッド | 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法 |
| WO2013041878A1 (en) | 2011-09-23 | 2013-03-28 | Oxford Nanopore Technologies Limited | Analysis of a polymer comprising polymer units |
| JP6226869B2 (ja) | 2011-10-21 | 2017-11-08 | オックスフォード ナノポール テクノロジーズ リミテッド | 酵素法 |
| GB201202519D0 (en) | 2012-02-13 | 2012-03-28 | Oxford Nanopore Tech Ltd | Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules |
| EP3736339B1 (en) | 2012-02-16 | 2022-07-27 | Oxford Nanopore Technologies plc | Analysis of measurements of a polymer |
| CN112646019B (zh) | 2012-04-10 | 2022-08-16 | 牛津纳米孔科技公开有限公司 | 突变胞溶素孔 |
| AU2013291763B2 (en) | 2012-07-19 | 2019-06-20 | Oxford Nanopore Technologies Limited | Modified helicases |
| EP2875154B1 (en) | 2012-07-19 | 2017-08-23 | Oxford Nanopore Technologies Limited | SSB method for characterising a nucleic acid |
| GB201313121D0 (en) | 2013-07-23 | 2013-09-04 | Oxford Nanopore Tech Ltd | Array of volumes of polar medium |
| US9823235B2 (en) * | 2012-10-26 | 2017-11-21 | Oxford Nanopre Technologies Ltd. | Droplet interfaces |
| GB201222928D0 (en) | 2012-12-19 | 2013-01-30 | Oxford Nanopore Tech Ltd | Analysis of a polynucleotide |
| AU2014224432B2 (en) | 2013-03-08 | 2019-10-24 | Oxford Nanopore Technologies Limited | Enzyme stalling method |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| CA2927726A1 (en) | 2013-10-18 | 2015-04-23 | Oxford Nanopore Technologies Limited | Modified helicases for use in nucleic acid sequencing |
| GB201406155D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| EP3097210B2 (en) | 2014-01-22 | 2022-04-20 | Oxford Nanopore Technologies Limited | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| EP3126515B1 (en) | 2014-04-04 | 2018-08-29 | Oxford Nanopore Technologies Limited | Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| EP3137490B1 (en) | 2014-05-02 | 2021-01-27 | Oxford Nanopore Technologies Limited | Mutant pores |
| US10400014B2 (en) | 2014-09-01 | 2019-09-03 | Oxford Nanopore Technologies Ltd. | Mutant CsgG pores |
| US10266885B2 (en) | 2014-10-07 | 2019-04-23 | Oxford Nanopore Technologies Ltd. | Mutant pores |
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| CN115851894B (zh) | 2014-10-16 | 2025-06-27 | 牛津楠路珀尔科技股份有限公司 | 聚合物的分析 |
| GB201418469D0 (en) | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Method |
| GB201418512D0 (en) | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Electrical device with detachable components |
| GB201502809D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Mutant pore |
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| EP3283887B1 (en) | 2015-04-14 | 2021-07-21 | Katholieke Universiteit Leuven | Nanopores with internal protein adaptors |
| GB201508669D0 (en) | 2015-05-20 | 2015-07-01 | Oxford Nanopore Tech Ltd | Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown |
| AU2016369071B2 (en) | 2015-12-08 | 2022-05-19 | Katholieke Universiteit Leuven Ku Leuven Research & Development | Modified nanopores, compositions comprising the same, and uses thereof |
| US11333626B2 (en) | 2016-02-22 | 2022-05-17 | Hitachi, Ltd. | Biological sample analysis chip, biological sample analyzer and biological sample analysis method |
| KR102222192B1 (ko) | 2016-03-02 | 2021-03-02 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 기공 |
| JP7364333B2 (ja) | 2016-04-06 | 2023-10-18 | オックスフォード ナノポール テクノロジーズ ピーエルシー | 変異体ポア |
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| GB201611770D0 (en) | 2016-07-06 | 2016-08-17 | Oxford Nanopore Tech | Microfluidic device |
| GB201616590D0 (en) | 2016-09-29 | 2016-11-16 | Oxford Nanopore Technologies Limited | Method |
| JP2020504808A (ja) | 2016-10-26 | 2020-02-13 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | ナノポア配列決定用の集積回路およびフローセルのマルチチップパッケージング |
| GB201619930D0 (en) | 2016-11-24 | 2017-01-11 | Oxford Nanopore Tech | Apparatus and methods for controlling insertion of a membrane channel into a membrane |
| GB201620450D0 (en) | 2016-12-01 | 2017-01-18 | Oxford Nanopore Tech Ltd | Method |
| JP7236999B2 (ja) | 2016-12-22 | 2023-03-10 | イラミーナ インコーポレーテッド | フローセルパッケージおよびその製造方法 |
| AU2018218531B2 (en) | 2017-02-10 | 2021-01-28 | Oxford Nanopore Technologies Limited | Modified nanopores, compositions comprising the same, and uses thereof |
| JP2018155698A (ja) * | 2017-03-21 | 2018-10-04 | 株式会社東芝 | 分析チップ |
| GB201707138D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Machine learning analysis of nanopore measurements |
| GB201707140D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| AU2018288767B2 (en) | 2017-06-20 | 2022-05-26 | Illumina, Inc. | Nanopore sequencers |
| CN117106037B (zh) | 2017-06-30 | 2025-07-25 | 弗拉芒区生物技术研究所 | 新颖蛋白孔 |
| WO2019063959A1 (en) | 2017-09-28 | 2019-04-04 | Oxford Nanopore Technologies Limited | KIT OF FIRST AND SECOND PARTS DESIGNED TO BE CONNECTED TO ONE ANOTHER |
| US12011679B2 (en) * | 2017-11-17 | 2024-06-18 | International Business Machines Corporation | Single layer nanofluidic separator chip and fluidic processor |
| GB2568895B (en) | 2017-11-29 | 2021-10-27 | Oxford Nanopore Tech Ltd | Microfluidic device |
| GB2569630B (en) | 2017-12-21 | 2022-10-12 | Sharp Life Science Eu Ltd | Droplet Interfaces in Electro-wetting Devices |
| GB201801768D0 (en) | 2018-02-02 | 2018-03-21 | Oxford Nanopore Tech Ltd | Synthesis method |
| WO2019149626A1 (de) | 2018-02-02 | 2019-08-08 | Bayer Aktiengesellschaft | Bekämpfung resistenter schadorganismen |
| GB201807793D0 (en) | 2018-05-14 | 2018-06-27 | Oxford Nanopore Tech Ltd | Method |
| GB2574048B (en) | 2018-05-24 | 2021-06-16 | Oxford Nanopore Tech Ltd | Nanopore sensor component with electrostatic discharge protection |
| CN112203767B (zh) | 2018-05-24 | 2023-04-11 | 牛津纳米孔科技公司 | 电润湿装置中的液滴界面 |
| GB201809323D0 (en) | 2018-06-06 | 2018-07-25 | Oxford Nanopore Tech Ltd | Method |
| GB201814369D0 (en) | 2018-09-04 | 2018-10-17 | Oxford Nanopore Tech Ltd | Method for determining a polymersequence |
| JP7499761B2 (ja) | 2018-11-08 | 2024-06-14 | オックスフォード ナノポール テクノロジーズ ピーエルシー | 細孔 |
| GB201818216D0 (en) | 2018-11-08 | 2018-12-26 | Oxford Nanopore Tech Ltd | Pore |
| WO2020109800A1 (en) | 2018-11-28 | 2020-06-04 | Oxford Nanopore Technologies Ltd. | Sensing system and method of operation |
| GB201819378D0 (en) | 2018-11-28 | 2019-01-09 | Oxford Nanopore Tech Ltd | Analysis of nanopore signal using a machine-learning technique |
| GB201821155D0 (en) | 2018-12-21 | 2019-02-06 | Oxford Nanopore Tech Ltd | Method |
| KR20210138594A (ko) | 2019-03-12 | 2021-11-19 | 옥스포드 나노포어 테크놀로지즈 피엘씨 | 나노포어 감지 디바이스 및 이를 작동하는 방법 및 형성하는 방법 |
| GB2586339B (en) * | 2019-03-19 | 2021-12-01 | Oxford Nanopore Tech Ltd | Current measurement apparatus, molecular entity sensing apparatus, method of measuring a current, method of sensing a molecular entity |
| GB2580988B (en) | 2019-03-19 | 2022-04-13 | Oxford Nanopore Tech Ltd | Current measurement apparatus, molecular entity sensing apparatus, method of measuring a current, method of sensing a molecular entity |
| CA3134796A1 (en) | 2019-04-09 | 2020-10-15 | Oxford Nanopore Technologies Limited | Pore |
| GB201907244D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| GB201907246D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| GB201907243D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Sensing interactions between molecular entities and nanapores |
| GB201915480D0 (en) * | 2019-10-25 | 2019-12-11 | Oxford Nanopore Tech Ltd | Improved nanopore sensing device, components and method of manufacture |
| GB201917060D0 (en) | 2019-11-22 | 2020-01-08 | Oxford Nanopore Tech Ltd | Method |
| CN118011004A (zh) | 2019-12-02 | 2024-05-10 | 牛津纳米孔科技公开有限公司 | 使用纳米孔表征靶多肽的方法 |
| WO2021111987A1 (ja) | 2019-12-03 | 2021-06-10 | 国立研究開発法人科学技術振興機構 | ナノポア構造体、ナノポア構造体を含む塩基配列解析装置 |
| GB201917742D0 (en) | 2019-12-04 | 2020-01-15 | Oxford Nanopore Tech Ltd | Method |
| GB201917832D0 (en) | 2019-12-05 | 2020-01-22 | Oxford Nanopore Tech Ltd | Microfluidic device for preparing and analysing a test liquid |
| US10961563B1 (en) * | 2019-12-19 | 2021-03-30 | Robert Bosch Gmbh | Nanoscale topography system for use in DNA sequencing and method for fabrication thereof |
| JPWO2021166597A1 (enExample) * | 2020-02-19 | 2021-08-26 | ||
| GB202004944D0 (en) | 2020-04-03 | 2020-05-20 | King S College London | Method |
| GB202016874D0 (en) * | 2020-10-23 | 2020-12-09 | Oxford Nanopore Tech Ltd | Nanopore support structure and manufacture thereof |
| CN115698331A (zh) | 2020-06-18 | 2023-02-03 | 牛津纳米孔科技公开有限公司 | 使用检测器选择性地表征多核苷酸的方法 |
| GB202009349D0 (en) | 2020-06-18 | 2020-08-05 | Oxford Nanopore Tech Ltd | Method |
| EP4168583A2 (en) | 2020-06-18 | 2023-04-26 | Oxford Nanopore Technologies plc | Method of characterising a polynucleotide moving through a nanopore |
| WO2022013551A1 (en) | 2020-07-17 | 2022-01-20 | Oxford Nanopore Technologies Limited | Nanopore sensing device |
| WO2022020461A1 (en) | 2020-07-22 | 2022-01-27 | Oxford Nanopore Technologies Inc. | Solid state nanopore formation |
| US20220059187A1 (en) | 2020-08-07 | 2022-02-24 | Oxford Nanopore Technologies Limited | Methods of detecting nucleic acid barcodes |
| GB202015993D0 (en) | 2020-10-08 | 2020-11-25 | Oxford Nanopore Tech Ltd | Method |
| WO2022227080A1 (zh) * | 2021-04-30 | 2022-11-03 | 成都齐碳科技有限公司 | 孔阵列层结构、芯片装置、纳米孔测序装置和成膜方法以及用途 |
| CN117337333A (zh) | 2021-05-19 | 2024-01-02 | 牛津纳米孔科技公开有限公司 | 用于补体链测序的方法 |
| GB202107192D0 (en) | 2021-05-19 | 2021-06-30 | Oxford Nanopore Tech Ltd | Method |
| GB202107354D0 (en) | 2021-05-24 | 2021-07-07 | Oxford Nanopore Tech Ltd | Method |
| CN113070113B (zh) * | 2021-06-03 | 2021-08-20 | 成都齐碳科技有限公司 | 芯片结构、成膜方法、纳米孔测序装置及应用 |
| CN113061531B (zh) * | 2021-06-03 | 2021-08-20 | 成都齐碳科技有限公司 | 芯片结构、芯片组件、成膜方法、纳米孔测序装置及应用 |
| CN113426499B (zh) * | 2021-07-08 | 2022-10-14 | 成都齐碳科技有限公司 | 微结构、生物芯片、成膜方法、基因测序装置及其应用 |
| US20250006308A1 (en) | 2021-11-29 | 2025-01-02 | Oxford Nanopore Technologies Plc | Nanopore measurement signal analysis |
| CN116297721A (zh) * | 2021-12-21 | 2023-06-23 | 成都齐碳科技有限公司 | 成膜方法、包含膜的系统及应用 |
| GB202118939D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Plc | Pore |
| GB202118908D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| GB202118906D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| GB202205617D0 (en) | 2022-04-14 | 2022-06-01 | Oxford Nanopore Tech Plc | Novel modified protein pores and enzymes |
| CN114908358B8 (zh) * | 2022-04-14 | 2024-06-21 | 孔确(成都)科技有限公司 | 一种两亲性分子层的制备方法及装置 |
| CN117779058A (zh) * | 2022-04-14 | 2024-03-29 | 广州孔确基因科技有限公司 | 一种两亲性分子层的制备方法及装置 |
| WO2023222657A1 (en) | 2022-05-17 | 2023-11-23 | Oxford Nanopore Technologies Plc | Method and adaptors |
| GB202207267D0 (en) | 2022-05-18 | 2022-06-29 | Oxford Nanopore Tech Plc | Calibration and profiling of a nanopore array device |
| CN114751363B (zh) * | 2022-06-14 | 2022-09-13 | 成都齐碳科技有限公司 | 孔阵列层结构、预涂方法、成膜方法及相关装置 |
| WO2023240427A1 (zh) * | 2022-06-14 | 2023-12-21 | 成都齐碳科技有限公司 | 孔阵列层结构、预涂方法、成膜方法及测序装置 |
| KR20250044691A (ko) | 2022-08-09 | 2025-04-01 | 옥스포드 나노포어 테크놀로지즈 피엘씨 | 신규 포어 단량체 및 포어 |
| GB202211607D0 (en) | 2022-08-09 | 2022-09-21 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| GB202211602D0 (en) | 2022-08-09 | 2022-09-21 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| KR20250048551A (ko) | 2022-08-09 | 2025-04-09 | 옥스포드 나노포어 테크놀로지즈 피엘씨 | 신규 포어 |
| GB202215442D0 (en) | 2022-10-19 | 2022-11-30 | Oxford Nanopore Tech Plc | Analysis of a polymer |
| EP4608850A2 (en) | 2022-10-28 | 2025-09-03 | Oxford Nanopore Technologies PLC | Pore monomers and pores |
| GB202216162D0 (en) | 2022-10-31 | 2022-12-14 | Oxford Nanopore Tech Plc | Method |
| WO2024094966A1 (en) | 2022-11-01 | 2024-05-10 | Oxford Nanopore Technologies Plc | Biochemical analysis system and method of controlling a biochemical analysis system |
| WO2024099985A1 (en) | 2022-11-10 | 2024-05-16 | Bayer Aktiengesellschaft | Targeted crop protection product application based on genetic profiles |
| GB202216905D0 (en) | 2022-11-11 | 2022-12-28 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| GB202301095D0 (en) | 2023-01-25 | 2023-03-08 | Oxford Nanopore Tech Plc | Calibration of a nanopore array device |
| GB202302812D0 (en) | 2023-02-27 | 2023-04-12 | Oxford Nanopore Tech Plc | Nanopore sensor device |
| GB202302810D0 (en) | 2023-02-27 | 2023-04-12 | Oxford Nanopore Tech Plc | Nanopore sensor device |
| GB202302811D0 (en) | 2023-02-27 | 2023-04-12 | Oxford Nanopore Tech Plc | Nanopore sensor device |
| GB202302813D0 (en) | 2023-02-27 | 2023-04-12 | Oxford Nanopore Tech Plc | Nanopore sensor device |
| GB202304324D0 (en) | 2023-03-24 | 2023-05-10 | Oxford Nanopore Tech Plc | Method and kits |
| GB202305524D0 (en) | 2023-04-14 | 2023-05-31 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| WO2024227837A1 (en) * | 2023-05-04 | 2024-11-07 | F. Hoffmann-La Roche Ag | Hybrid triblock copolymer membrane compositions and methods for nanopore sequencing |
| GB202307141D0 (en) | 2023-05-12 | 2023-06-28 | Oxford Nanopore Tech Plc | Modified helicases |
| GB202307494D0 (en) | 2023-05-18 | 2023-07-05 | Oxford Nanopore Tech Ltd | Nanopore sequencing and preparation modular instrument |
| GB202307486D0 (en) | 2023-05-18 | 2023-07-05 | Oxford Nanopore Tech Plc | Method |
| WO2025074983A1 (ja) * | 2023-10-02 | 2025-04-10 | 地方独立行政法人神奈川県立産業技術総合研究所 | 脂質二重膜形成用隔壁並びにそれを含む脂質二重膜形成デバイス及びそれを用いた脂質二重膜形成方法 |
| GB202315935D0 (en) | 2023-10-18 | 2023-11-29 | Oxford Nanopore Tech Ltd | Novel enzymes |
| GB202317028D0 (en) | 2023-11-06 | 2023-12-20 | Oxford Nanopore Tech Ltd | Method |
| GB202401864D0 (en) | 2024-02-12 | 2024-03-27 | Fund Centre De Regulacio Genòmica | A method of detecting non-canonical bases in sequencing data |
| GB202405960D0 (en) | 2024-04-29 | 2024-06-12 | Oxford Nanopore Tech Plc | Photo-active conjugates and their uses |
| GB202407044D0 (en) | 2024-05-17 | 2024-07-03 | Oxford Nanopore Tech Plc | Polymer analysis using transformer neural network |
| GB202407097D0 (en) * | 2024-05-17 | 2024-07-03 | Oxford Nanopore Tech Plc | A flow cell and a method |
| GB202407055D0 (en) | 2024-05-17 | 2024-07-03 | Oxford Nanopore Tech Plc | Enhanced polynucleotide sequencing |
| GB202407099D0 (en) * | 2024-05-17 | 2024-07-03 | Oxford Nanopore Tech Plc | A flow cell and a method |
| GB202407216D0 (en) | 2024-05-21 | 2024-07-03 | Oxford Nanopore Tech Ltd | A flow cell |
| GB202407228D0 (en) | 2024-05-21 | 2024-07-03 | Oxford Nanopore Tech Plc | Method |
| GB202407247D0 (en) | 2024-05-21 | 2024-07-03 | Oxford Nanopore Tech Plc | Method |
Family Cites Families (194)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3799743A (en) | 1971-11-22 | 1974-03-26 | Alexander James | Stable lysis responsive lipid bilayer |
| JPS5274882A (en) | 1975-12-18 | 1977-06-23 | Fujitsu Ltd | Superhigh density liquid contact connector |
| US4154795A (en) * | 1976-07-23 | 1979-05-15 | Dynatech Holdings Limited | Microtest plates |
| ES2150428T3 (es) | 1987-04-27 | 2000-12-01 | Unilever Nv | Ensayos de union especifica. |
| US4874500A (en) | 1987-07-15 | 1989-10-17 | Sri International | Microelectrochemical sensor and sensor array |
| DE68926118T2 (de) | 1988-08-18 | 1996-08-22 | Australian Membrane And Biotechnology Research Institute Ltd. Commonwealth Scientific And Industrial Research Organization, North Ryde, Neusuedwales | Verbesserungen an der empfindlichkeit und der selektivität von ionenkanalmembranbiosensoren |
| GB8924338D0 (en) | 1989-10-28 | 1989-12-13 | Atomic Energy Authority Uk | Electrodes |
| JPH0414773A (ja) | 1990-05-07 | 1992-01-20 | Fujitsu Ltd | 電気的接続部材および装置 |
| JPH04127066A (ja) | 1990-09-18 | 1992-04-28 | Fujitsu Ltd | 信号端子接続方法および信号端子接続装置 |
| JPH04215052A (ja) | 1990-10-23 | 1992-08-05 | Yokogawa Electric Corp | 脂質膜型化学物質センサ |
| CA2077286C (en) | 1991-09-10 | 1996-08-06 | Tatsuo Chiyonobu | Electrical connecting method |
| US5605662A (en) | 1993-11-01 | 1997-02-25 | Nanogen, Inc. | Active programmable electronic devices for molecular biological analysis and diagnostics |
| US5290240A (en) | 1993-02-03 | 1994-03-01 | Pharmetrix Corporation | Electrochemical controlled dispensing assembly and method for selective and controlled delivery of a dispensing fluid |
| CA2157503A1 (en) | 1993-03-05 | 1994-09-15 | John M. Riviello | Pulsed electrochemical detection using polymer electroactive electrodes |
| WO1994025862A1 (en) | 1993-05-04 | 1994-11-10 | Washington State University Research Foundation | Biosensor substrate for mounting bilayer lipid membrane containing a receptor |
| US6095148A (en) | 1995-11-03 | 2000-08-01 | Children's Medical Center Corporation | Neuronal stimulation using electrically conducting polymers |
| JP3822946B2 (ja) | 1996-05-30 | 2006-09-20 | 三洋電機株式会社 | 二分子膜素子 |
| JP3961588B2 (ja) | 1996-06-18 | 2007-08-22 | 日本メジフィジックス株式会社 | 放射性核種溶出装置包装用内装材 |
| EP0938674B1 (de) | 1996-11-16 | 2005-06-01 | NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen in Reutlingen Stiftung Bürgerlichen Rechts | Mikroelementenanordnung, verfahren zum kontaktieren von in einer flüssigen umgebung befindlichen zellen und verfahren zum herstellen einer mikroelementenanordnung |
| US6503452B1 (en) | 1996-11-29 | 2003-01-07 | The Board Of Trustees Of The Leland Stanford Junior University | Biosensor arrays and methods |
| US7169272B2 (en) | 1997-04-30 | 2007-01-30 | Board Of Trustees Of The University Of Arkansas | Microfabricated recessed disk microelectrodes: characterization in static and convective solutions |
| US7144486B1 (en) | 1997-04-30 | 2006-12-05 | Board Of Trustees Of The University Of Arkansas | Multilayer microcavity devices and methods |
| GB9712386D0 (en) | 1997-06-14 | 1997-08-13 | Univ Coventry | Biosensor |
| US6300138B1 (en) | 1997-08-01 | 2001-10-09 | Qualigen, Inc. | Methods for conducting tests |
| US6129823A (en) | 1997-09-05 | 2000-10-10 | Abbott Laboratories | Low volume electrochemical sensor |
| US6483931B2 (en) | 1997-09-11 | 2002-11-19 | Stmicroelectronics, Inc. | Electrostatic discharge protection of a capacitve type fingerprint sensing array |
| US7244349B2 (en) | 1997-12-17 | 2007-07-17 | Molecular Devices Corporation | Multiaperture sample positioning and analysis system |
| US20020144905A1 (en) | 1997-12-17 | 2002-10-10 | Christian Schmidt | Sample positioning and analysis system |
| WO1999041401A1 (en) | 1998-02-17 | 1999-08-19 | University Of Wales College Of Medicine | Method and apparatus for introducing substances into the cell plasma membrane and/or cytosol |
| CA2348002A1 (en) | 1998-10-27 | 2000-05-04 | Malcolm W. Mcgeoch | Biological ion channels in nanofabricated detectors |
| US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
| AU5646800A (en) | 1999-03-02 | 2000-09-21 | Helix Biopharma Corporation | Card-based biosensor device |
| US7223364B1 (en) | 1999-07-07 | 2007-05-29 | 3M Innovative Properties Company | Detection article having fluid control film |
| US6916488B1 (en) | 1999-11-05 | 2005-07-12 | Biocure, Inc. | Amphiphilic polymeric vesicles |
| AU2001234996A1 (en) | 2000-02-11 | 2001-08-20 | Yale University | Planar patch clamp electrodes |
| CA2407973C (en) | 2000-05-03 | 2011-06-07 | Jen-Jr Gau | Biological identification system with integrated sensor chip |
| WO2002024862A2 (en) | 2000-09-19 | 2002-03-28 | Cytion S.A. | Sample positioning and analysis system |
| AU2001293676B2 (en) | 2000-10-02 | 2006-07-27 | Sophion Bioscience A/S | System for electrophysiological measurements |
| EP1328798B1 (en) | 2000-10-25 | 2006-03-01 | Xiamen University | Electrophoretic separating device and method for using the device |
| GB0026276D0 (en) | 2000-10-27 | 2000-12-13 | Univ Ulster | Method for chlorine plasma modification of silver electrodes |
| CN1310379C (zh) | 2001-01-16 | 2007-04-11 | 郑慧光 | 一种提高电线路便拆式连接的传导性能的方法 |
| US6913697B2 (en) | 2001-02-14 | 2005-07-05 | Science & Technology Corporation @ Unm | Nanostructured separation and analysis devices for biological membranes |
| AU2002338371A1 (en) | 2001-04-06 | 2002-10-21 | The Regents Of The University Of California | Silicon-wafer based devices and methods for analyzing biological material |
| DE60216076T2 (de) * | 2001-04-26 | 2007-06-21 | Varian, Inc., Palo Alto | Hohlfasermembran probenpräparationsanordnungen |
| US7077939B1 (en) | 2001-06-18 | 2006-07-18 | The Texas A&M University System | Method and apparatus for nanoparticle transport and detection |
| US6863833B1 (en) | 2001-06-29 | 2005-03-08 | The Board Of Trustees Of The Leland Stanford Junior University | Microfabricated apertures for supporting bilayer lipid membranes |
| DK1412725T3 (en) | 2001-06-29 | 2019-03-25 | Meso Scale Technologies Llc | Multi-well plates for LUMINESCENSE TEST MEASUREMENTS |
| US6890409B2 (en) | 2001-08-24 | 2005-05-10 | Applera Corporation | Bubble-free and pressure-generating electrodes for electrophoretic and electroosmotic devices |
| US20050230272A1 (en) | 2001-10-03 | 2005-10-20 | Lee Gil U | Porous biosensing device |
| US7374944B2 (en) | 2001-10-03 | 2008-05-20 | Purdue Research Foundation | Device and bioanalytical method utilizing asymmetric biofunctionalized membrane |
| ATE509272T1 (de) * | 2001-11-09 | 2011-05-15 | 3Dbiosurfaces Technologies Llc | Substrate mit hochliegendem oberflächenbereich für mikroarrays sowie verfahren zur herstellung davon |
| US6783645B2 (en) | 2001-12-18 | 2004-08-31 | Dionex Corporation | Disposable working electrode for an electrochemical cell |
| JP4127066B2 (ja) | 2002-02-08 | 2008-07-30 | 東レ株式会社 | 易染色性ポリエステル繊維およびその製造方法 |
| US20030224523A1 (en) | 2002-05-30 | 2003-12-04 | Thornberg John Herbert | Cartridge arrangement, fluid analyzer arrangement, and methods |
| FR2844052B1 (fr) | 2002-08-28 | 2005-07-01 | Commissariat Energie Atomique | Dispositif de mesure de l'activite electrique d'elements biologiques et ses applications |
| US8679517B2 (en) | 2002-09-26 | 2014-03-25 | Palmaz Scientific, Inc. | Implantable materials having engineered surfaces made by vacuum deposition and method of making same |
| JP2004147534A (ja) * | 2002-10-29 | 2004-05-27 | Koji Nishimura | 免疫調節作用関連の新規遺伝子及びＴｈ１ヘルパーＴ細胞とＴｈ２ヘルパーＴ細胞のバランスを調べる検査方法 |
| JP2004158330A (ja) | 2002-11-07 | 2004-06-03 | Toshiba Corp | 半導体装置のテストソケット |
| EP1419818B1 (de) | 2002-11-14 | 2013-10-30 | Boehringer Ingelheim microParts GmbH | Vorrichtung zum schrittweisen Transport von Flüssigkeit unter Ausnutzung von Kapillarkräften |
| US20040115654A1 (en) * | 2002-12-16 | 2004-06-17 | Intel Corporation | Laser exposure of photosensitive masks for DNA microarray fabrication |
| CN1232813C (zh) | 2003-03-13 | 2005-12-21 | 东南大学 | 制备纳米管探针针尖的方法 |
| US7745116B2 (en) | 2003-04-08 | 2010-06-29 | Pacific Biosciences Of California, Inc. | Composition and method for nucleic acid sequencing |
| JP3801617B2 (ja) | 2003-06-27 | 2006-07-26 | 松下電器産業株式会社 | 薬理測定装置およびシステム並びにそれに用いるウェル容器 |
| US7347921B2 (en) | 2003-07-17 | 2008-03-25 | Agilent Technologies, Inc. | Apparatus and method for threading a biopolymer through a nanopore |
| JP4394917B2 (ja) | 2003-09-19 | 2010-01-06 | 独立行政法人科学技術振興機構 | 人工脂質二重膜を有する電流測定装置 |
| JP4394916B2 (ja) | 2003-09-19 | 2010-01-06 | 独立行政法人科学技術振興機構 | 人工脂質二重膜の形成装置および人工脂質二重膜の形成方法、並びにその利用 |
| JP3769622B2 (ja) | 2003-09-22 | 2006-04-26 | 国立大学法人 東京大学 | 人工脂質膜の形成方法とそのための脂質平面膜形成装置 |
| EP1678488A1 (en) | 2003-10-22 | 2006-07-12 | Ambri Limited | Novel sensor configuration |
| EP1535667A1 (en) | 2003-11-28 | 2005-06-01 | Sysmex Corporation | Analyzer, assay cartridge and analyzing method |
| JP2005164276A (ja) | 2003-11-28 | 2005-06-23 | Sysmex Corp | 分析装置および測定ユニット |
| KR100540143B1 (ko) | 2003-12-22 | 2006-01-10 | 한국전자통신연구원 | 미소 유체 제어소자 및 미소 유체의 제어 방법 |
| EP1548444A1 (en) | 2003-12-23 | 2005-06-29 | Paul Scherrer Institut | An assay chip, and uses of said assay chip to determine molecular structures and functions |
| US8039247B2 (en) | 2004-01-21 | 2011-10-18 | Japan Science And Technology Agency | Method of forming planar lipid double membrane for membrane protein analysis and apparatus therefor |
| US8101431B2 (en) | 2004-02-27 | 2012-01-24 | Board Of Regents, The University Of Texas System | Integration of fluids and reagents into self-contained cartridges containing sensor elements and reagent delivery systems |
| US20060257941A1 (en) | 2004-02-27 | 2006-11-16 | Mcdevitt John T | Integration of fluids and reagents into self-contained cartridges containing particle and membrane sensor elements |
| JP2005300460A (ja) | 2004-04-15 | 2005-10-27 | Sony Corp | 相互作用検出部と該検出部を備えるバイオアッセイ用基板、並びに媒質滴下方法と媒質の水分の蒸発を防止する方法 |
| WO2005124888A1 (en) | 2004-06-08 | 2005-12-29 | President And Fellows Of Harvard College | Suspended carbon nanotube field effect transistor |
| US20060292649A1 (en) | 2004-07-19 | 2006-12-28 | Cell Biosciences Inc. | Methods and apparatus for reference lab diagnostics |
| GB2431013B (en) | 2004-07-23 | 2008-05-21 | Electronic Bio Sciences Llc | Method and apparatus for sensing a time varying current passing through an ion channel |
| US7163830B2 (en) | 2004-10-12 | 2007-01-16 | Salmon Peter C | Method for temporarily engaging electronic component for test |
| PL1859330T3 (pl) | 2005-01-28 | 2013-01-31 | Univ Duke | Urządzenia i sposoby manipulacji kropelkami na płytkach obwodów drukowanych |
| KR100698961B1 (ko) | 2005-02-04 | 2007-03-26 | 주식회사 아이센스 | 전기화학적 바이오센서 |
| CA2597496A1 (en) | 2005-02-10 | 2006-08-17 | Chempaq A/S | Dual sample cartridge and method for characterizing particles in liquid |
| GB0505971D0 (en) | 2005-03-23 | 2005-04-27 | Isis Innovation | Delivery of molecules to a lipid bilayer |
| WO2006104639A2 (en) | 2005-03-29 | 2006-10-05 | Stanford University | Device comprising array of micro-or nano-reservoirs |
| US20060228402A1 (en) | 2005-04-08 | 2006-10-12 | Charite-Universitatsmedizin Berlin | Techniques for forming a lipid bilayer membrane |
| WO2006113550A2 (en) | 2005-04-15 | 2006-10-26 | Genencor International, Inc. | Viral nucleoprotein detection using an ion channel switch biosensor |
| JP4953044B2 (ja) | 2005-05-09 | 2012-06-13 | 財団法人生産技術研究奨励会 | 脂質二重膜の形成方法およびその装置 |
| US20070238184A1 (en) | 2005-06-16 | 2007-10-11 | The Regents Of The University Of California | Amyloid beta protein channel structure and uses thereof in identifying potential drug molecules for neurodegenerative diseases |
| JP5114702B2 (ja) * | 2005-07-29 | 2013-01-09 | 国立大学法人 東京大学 | 両親媒性単分子膜の接触による二分子膜の形成方法およびその装置 |
| US8005526B2 (en) | 2005-08-31 | 2011-08-23 | The Regents Of The University Of Michigan | Biologically integrated electrode devices |
| US8986781B2 (en) | 2005-10-27 | 2015-03-24 | Corning Incorporated | Immobilized multi-layer artificial membrane for permeability measurements (PAMPA) |
| WO2007049576A1 (ja) | 2005-10-28 | 2007-05-03 | Kuraray Co., Ltd. | 細胞培養容器及び細胞培養方法 |
| EP1987275A1 (en) | 2006-02-13 | 2008-11-05 | Koninklijke Philips Electronics N.V. | Microfluidic device for molecular diagnostic applications |
| RU2422204C2 (ru) | 2006-03-20 | 2011-06-27 | Конинклейке Филипс Электроникс Н.В. | Платформа система в корпусе для электронно-микрофлюидных устройств |
| JP2009156572A (ja) | 2006-04-06 | 2009-07-16 | National Institutes Of Natural Sciences | イオンチャンネルタンパク質バイオセンサー |
| US20070298511A1 (en) | 2006-04-27 | 2007-12-27 | The Texas A&M University System | Nanopore sensor system |
| AU2007251545A1 (en) | 2006-05-17 | 2007-11-22 | Eppendorf Array Technologies S.A. | Identification and quantification of a plurality of biological (micro)organisms or their components |
| GB0614835D0 (en) | 2006-07-26 | 2006-09-06 | Isis Innovation | Formation of bilayers of amphipathic molecules |
| US9018019B2 (en) | 2006-10-04 | 2015-04-28 | President And Fellows Of Harvard College | Engineered conductive polymer films to mediate biochemical interactions |
| JP2008194573A (ja) | 2007-02-09 | 2008-08-28 | Matsushita Electric Ind Co Ltd | 脂質二重膜形成方法 |
| GB2446823A (en) | 2007-02-20 | 2008-08-27 | Oxford Nanolabs Ltd | Formulation of lipid bilayers |
| NZ579083A (en) | 2007-02-20 | 2012-07-27 | Oxford Nanopore Tech Ltd | Lipid bilayer sensor system |
| US20080254995A1 (en) | 2007-02-27 | 2008-10-16 | Drexel University | Nanopore arrays and sequencing devices and methods thereof |
| AU2008236694B2 (en) | 2007-04-04 | 2014-01-23 | The Regents Of The University Of California | Compositions, devices, systems, and methods for using a nanopore |
| CN100448007C (zh) | 2007-04-19 | 2008-12-31 | 浙江大学 | 一种网格状静电放电防护器件 |
| CN103495439B (zh) | 2007-05-04 | 2015-09-16 | 欧普科诊断有限责任公司 | 流体连接器和微流体系统 |
| EP2169049B1 (en) * | 2007-06-18 | 2018-01-17 | Kuraray Co., Ltd. | Cell culture container and cell culture method |
| CN100523799C (zh) | 2007-06-27 | 2009-08-05 | 浙江大学 | 聚电解质/本征导电聚合物复合湿敏元件及其制作方法 |
| GB0716264D0 (en) | 2007-08-21 | 2007-09-26 | Isis Innovation | Bilayers |
| WO2009035647A1 (en) | 2007-09-12 | 2009-03-19 | President And Fellows Of Harvard College | High-resolution molecular graphene sensor comprising an aperture in the graphene layer |
| WO2009039286A2 (en) * | 2007-09-18 | 2009-03-26 | Primorigen Biosciences, Llc | Frameless multiplexed microarrays |
| JP5441142B2 (ja) | 2007-11-26 | 2014-03-12 | 国立大学法人 東京大学 | マイクロ流体による平面脂質二重膜アレイ及びその平面脂質二重膜を用いた分析方法 |
| WO2009073622A1 (en) | 2007-11-30 | 2009-06-11 | Electronic Bio Sciences, Llc | Method and apparatus for single side bilayer formation |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| US8697007B2 (en) | 2008-08-06 | 2014-04-15 | The Trustees Of The University Of Pennsylvania | Biodetection cassette with automated actuator |
| US8669124B2 (en) | 2008-08-20 | 2014-03-11 | Nxp, B.V. | Apparatus and method for molecule detection using nanopores |
| US20100190146A1 (en) | 2009-01-29 | 2010-07-29 | Bynum Magdalena A | Microfluidic Glycan Analysis |
| CA2750874A1 (en) | 2009-01-30 | 2010-08-05 | Oxford Nanopore Technologies Limited | Hybridization linkers |
| JP2010186677A (ja) | 2009-02-13 | 2010-08-26 | Ritsumeikan | 導電構造、アクチュエータ、可変抵抗器、回動部材、回動式コネクタ、電動機、コントローラ、回転情報検出装置、ストローク検出装置、および導電端子の製造方法 |
| US8986928B2 (en) | 2009-04-10 | 2015-03-24 | Pacific Biosciences Of California, Inc. | Nanopore sequencing devices and methods |
| JP5781502B2 (ja) | 2009-04-20 | 2015-09-24 | オックスフォード ナノポール テクノロジーズ リミテッド | 脂質二重層センサアレイ |
| US8558554B2 (en) | 2009-04-21 | 2013-10-15 | Johnstech International Corporation | Electrically conductive Kelvin contacts for microcircuit tester |
| GB0909923D0 (en) | 2009-06-09 | 2009-07-22 | Oxford Gene Tech Ip Ltd | Picowell capture devices for analysing single cells or other particles |
| US8174279B2 (en) | 2009-08-21 | 2012-05-08 | Freescale Semiconductor, Inc. | Socket connector for connection lead of semiconductor device under test with tester |
| EP3196645B1 (en) | 2009-09-18 | 2019-06-19 | President and Fellows of Harvard College | Bare single-layer graphene membrane having a nanopore enabling high-sensitivity molecular detection and analysis |
| WO2011063332A2 (en) * | 2009-11-23 | 2011-05-26 | 3M Innovative Properties Company | Microwell array articles and methods of use |
| BR112012013074B1 (pt) | 2009-12-01 | 2018-09-18 | Oxford Nanopore Technologies Limited | instrumento de análise e módulo para realizar análise bioquímica, e, método para operar um instrumento de análise para realizar análise bioquímica |
| WO2011090556A1 (en) | 2010-01-19 | 2011-07-28 | Verinata Health, Inc. | Methods for determining fraction of fetal nucleic acid in maternal samples |
| US20110287414A1 (en) | 2010-02-08 | 2011-11-24 | Genia Technologies, Inc. | Systems and methods for identifying a portion of a molecule |
| US8324914B2 (en) | 2010-02-08 | 2012-12-04 | Genia Technologies, Inc. | Systems and methods for characterizing a molecule |
| KR20110100963A (ko) | 2010-03-05 | 2011-09-15 | 삼성전자주식회사 | 미세 유동 장치 및 이를 이용한 표적 핵산의 염기 서열 결정 방법 |
| JP6159529B2 (ja) * | 2010-03-23 | 2017-07-05 | 株式会社クラレ | 多能性哺乳細胞を分化させる培養方法 |
| DE102010022929B4 (de) * | 2010-06-07 | 2013-07-18 | Albert-Ludwigs-Universität Freiburg | Verfahren zum Herstellen einer Bilipidschicht sowie Mikrostruktur und Messanordnung |
| WO2012033524A2 (en) | 2010-09-07 | 2012-03-15 | The Regents Of The University Of California | Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme |
| CN103370617B (zh) | 2010-10-01 | 2015-11-25 | 牛津纳米孔技术有限公司 | 生物化学分析设备和旋转阀 |
| KR101939420B1 (ko) | 2011-02-11 | 2019-01-16 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 세공 |
| EP2694671B1 (en) | 2011-04-04 | 2021-01-06 | President and Fellows of Harvard College | Nanopore sensor comprising a device for measuring the local electrical potential |
| JP2012247231A (ja) | 2011-05-25 | 2012-12-13 | Fujikura Kasei Co Ltd | 検査器具 |
| CN102263104B (zh) | 2011-06-16 | 2013-04-17 | 北京大学 | Mos结构的esd保护器件 |
| CA2944478A1 (en) | 2011-07-20 | 2013-01-24 | The Regents Of The University Of California | Dual-pore device |
| US10761043B2 (en) | 2011-07-22 | 2020-09-01 | The Trustees Of The University Of Pennsylvania | Graphene-based nanopore and nanostructure devices and methods for macromolecular analysis |
| JP2014521956A (ja) | 2011-07-27 | 2014-08-28 | ザ ボード オブ トラスティーズ オブ ザ ユニヴァーシティー オブ イリノイ | 生体分子を特徴付けるためのナノ細孔センサー |
| JP5670278B2 (ja) | 2011-08-09 | 2015-02-18 | 株式会社日立ハイテクノロジーズ | ナノポア式分析装置 |
| WO2013041878A1 (en) | 2011-09-23 | 2013-03-28 | Oxford Nanopore Technologies Limited | Analysis of a polymer comprising polymer units |
| JP6226869B2 (ja) | 2011-10-21 | 2017-11-08 | オックスフォード ナノポール テクノロジーズ リミテッド | 酵素法 |
| US9696277B2 (en) | 2011-11-14 | 2017-07-04 | The Regents Of The University Of California | Two-chamber dual-pore device |
| EP2798083B1 (en) | 2011-12-29 | 2017-08-09 | Oxford Nanopore Technologies Limited | Method for characterising a polynucelotide by using a xpd helicase |
| KR102086182B1 (ko) | 2011-12-29 | 2020-03-06 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 효소 방법 |
| JP5898969B2 (ja) | 2012-01-18 | 2016-04-06 | 株式会社日立製作所 | 半導体装置 |
| JP5912582B2 (ja) | 2012-01-27 | 2016-04-27 | ローム株式会社 | 包材入り液体試薬内蔵型マイクロチップおよびその使用方法 |
| GB201202519D0 (en) | 2012-02-13 | 2012-03-28 | Oxford Nanopore Tech Ltd | Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules |
| US8541849B2 (en) | 2012-02-14 | 2013-09-24 | Genia Technologies, Inc. | Noise shielding techniques for ultra low current measurements in biochemical applications |
| EP3736339B1 (en) | 2012-02-16 | 2022-07-27 | Oxford Nanopore Technologies plc | Analysis of measurements of a polymer |
| CN112646019B (zh) | 2012-04-10 | 2022-08-16 | 牛津纳米孔科技公开有限公司 | 突变胞溶素孔 |
| US9758821B2 (en) | 2012-04-17 | 2017-09-12 | International Business Machines Corporation | Graphene transistor gated by charges through a nanopore for bio-molecular sensing and DNA sequencing |
| JP5692164B2 (ja) | 2012-05-22 | 2015-04-01 | ウシオ電機株式会社 | マイクロチップへの試薬供給方法及びマイクロチップへの試薬供給装置 |
| JP6172711B2 (ja) | 2012-07-05 | 2017-08-02 | 国立研究開発法人理化学研究所 | マイクロチップ用の流体制御デバイスおよびその利用 |
| AU2013291763B2 (en) | 2012-07-19 | 2019-06-20 | Oxford Nanopore Technologies Limited | Modified helicases |
| US9797009B2 (en) | 2012-07-19 | 2017-10-24 | Oxford Nanopore Technologies Limited | Enzyme construct |
| EP3584010A1 (de) | 2012-07-30 | 2019-12-25 | NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen | Untersuchungsverfahren an und/oder mit einem biologischen probenmaterial |
| US10876157B2 (en) | 2012-09-27 | 2020-12-29 | The Trustees Of The University Of Pennsylvania | Insulated nanoelectrode-nanopore devices and related methods |
| US9823235B2 (en) | 2012-10-26 | 2017-11-21 | Oxford Nanopre Technologies Ltd. | Droplet interfaces |
| GB201313121D0 (en) | 2013-07-23 | 2013-09-04 | Oxford Nanopore Tech Ltd | Array of volumes of polar medium |
| EP2911791A4 (en) | 2012-10-29 | 2016-11-02 | Mbio Diagnostics Inc | BIOPARTICLE IDENTIFICATION SYSTEM, CARTRIDGE AND RELATED METHODS |
| WO2014132343A1 (ja) | 2013-02-26 | 2014-09-04 | 株式会社日立製作所 | Ｆｅｔアレイ基板、分析システム、及び方法 |
| EP4039733A1 (en) | 2013-03-14 | 2022-08-10 | Arkema, Inc. | Methods for crosslinking polymer compositions in the presence of atmospheric oxygen |
| JP2014190891A (ja) | 2013-03-28 | 2014-10-06 | Hitachi High-Technologies Corp | ナノポア式分析装置の電圧印加システム |
| GB2515104B (en) | 2013-06-14 | 2015-07-22 | Suunto Oy | A device and a method for assembling an electronic device and a flexible element for facilitating assembly of electronic components |
| US9279801B2 (en) | 2013-07-26 | 2016-03-08 | Axion Biosystems, Inc. | Devices, systems and methods for high-throughput electrophysiology |
| CN203466320U (zh) | 2013-09-20 | 2014-03-05 | 番禺得意精密电子工业有限公司 | 电连接器 |
| CA2927726A1 (en) | 2013-10-18 | 2015-04-23 | Oxford Nanopore Technologies Limited | Modified helicases for use in nucleic acid sequencing |
| US9828623B2 (en) | 2014-03-13 | 2017-11-28 | Illinois Institute Of Technology | Nanopore stochastic sensing of biomarkers |
| WO2015183871A1 (en) | 2014-05-27 | 2015-12-03 | Illumina, Inc. | Systems and methods for biochemical analysis including a base instrument and a removable cartridge |
| CN103995035A (zh) | 2014-05-29 | 2014-08-20 | 东南大学 | 用于碱基序列检测的多栅极石墨烯场效应管结构及制备方法 |
| JP6360568B2 (ja) | 2014-06-16 | 2018-07-18 | コーニンクレッカ フィリップス エヌ ヴェＫｏｎｉｎｋｌｉｊｋｅ Ｐｈｉｌｉｐｓ Ｎ．Ｖ． | 迅速な試料取り込みのためのカートリッジ |
| GB201418512D0 (en) | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Electrical device with detachable components |
| CN105701440B (zh) | 2014-12-11 | 2019-01-15 | 义隆电子股份有限公司 | 具静电防护的指纹感测器 |
| CN105701441B (zh) | 2014-12-15 | 2018-10-09 | 义隆电子股份有限公司 | 指纹感测器的感测方法及电路 |
| US9658190B2 (en) | 2014-12-18 | 2017-05-23 | Genia Technologies, Inc. | Printed electrode |
| US10794895B2 (en) | 2015-02-05 | 2020-10-06 | President And Fellows Of Harvard College | Nanopore sensor including fluidic passage |
| JP6830443B2 (ja) | 2015-04-24 | 2021-02-17 | メサ バイオテック，インク． | 流体検査用カセット |
| GB201508669D0 (en) | 2015-05-20 | 2015-07-01 | Oxford Nanopore Tech Ltd | Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown |
| JPWO2017061600A1 (ja) | 2015-10-08 | 2018-08-02 | 凸版印刷株式会社 | マイクロ流体デバイスおよび試料分析方法 |
| GB201611770D0 (en) | 2016-07-06 | 2016-08-17 | Oxford Nanopore Tech | Microfluidic device |
| CN205828393U (zh) | 2016-07-15 | 2016-12-21 | 中芯国际集成电路制造(北京)有限公司 | 一种用于静电放电保护的高密度栅极二极管 |
| CA3067993C (en) | 2017-06-21 | 2024-02-27 | Ontera Inc. | Dual pore-control and sensor device |
| WO2019063959A1 (en) | 2017-09-28 | 2019-04-04 | Oxford Nanopore Technologies Limited | KIT OF FIRST AND SECOND PARTS DESIGNED TO BE CONNECTED TO ONE ANOTHER |
| GB2568895B (en) | 2017-11-29 | 2021-10-27 | Oxford Nanopore Tech Ltd | Microfluidic device |
| CA3066469C (en) | 2018-02-16 | 2022-06-21 | Illumina, Inc. | Device for sequencing |
| GB2574048B (en) | 2018-05-24 | 2021-06-16 | Oxford Nanopore Tech Ltd | Nanopore sensor component with electrostatic discharge protection |
| KR20210138594A (ko) | 2019-03-12 | 2021-11-19 | 옥스포드 나노포어 테크놀로지즈 피엘씨 | 나노포어 감지 디바이스 및 이를 작동하는 방법 및 형성하는 방법 |
| WO2022013551A1 (en) | 2020-07-17 | 2022-01-20 | Oxford Nanopore Technologies Limited | Nanopore sensing device |
| US11940404B2 (en) | 2022-02-16 | 2024-03-26 | Western Digital Technologies, Inc. | Low noise amplifiers with shields for nanopore Applications |
-
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| GB201313121D0 (en) | 2013-09-04 |
| CA2889660A1 (en) | 2014-05-01 |
| US20250033016A1 (en) | 2025-01-30 |
| JP2015535179A (ja) | 2015-12-10 |
| US10814298B2 (en) | 2020-10-27 |
| US20210086160A1 (en) | 2021-03-25 |
| US12350637B2 (en) | 2025-07-08 |
| US20220023819A1 (en) | 2022-01-27 |
| CN109569458A (zh) | 2019-04-05 |
| CN104918696B (zh) | 2018-11-13 |
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