AU2013336429B2 - Formation of array of membranes and apparatus therefor - Google Patents

Formation of array of membranes and apparatus therefor Download PDF

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Publication number
AU2013336429B2
AU2013336429B2 AU2013336429A AU2013336429A AU2013336429B2 AU 2013336429 B2 AU2013336429 B2 AU 2013336429B2 AU 2013336429 A AU2013336429 A AU 2013336429A AU 2013336429 A AU2013336429 A AU 2013336429A AU 2013336429 B2 AU2013336429 B2 AU 2013336429B2
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medium
polar medium
volumes
polar
support
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AU2013336429A1 (en
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Pedro Miguel Ortiz BAHAMON
Clive Gavin Brown
Andrew John Heron
Jason Robert Hyde
Paul Raymond MACKETT
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Oxford Nanopore Technologies PLC
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Oxford Nanopore Technologies PLC
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Rigid containers without fluid transport within
    • B01L3/5088—Rigid containers without fluid transport within confining liquids at a location by surface tension, e.g. virtual wells on plates, wires
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00734—Lipids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00736—Non-biologic macromolecules, e.g. polymeric compounds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0642—Filling fluids into wells by specific techniques
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/974—Thrombin

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Clinical Laboratory Science (AREA)
  • Nanotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
AU2013336429A 2012-10-26 2013-10-23 Formation of array of membranes and apparatus therefor Active AU2013336429B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261718899P 2012-10-26 2012-10-26
US61/718,899 2012-10-26
GBGB1313121.4A GB201313121D0 (en) 2013-07-23 2013-07-23 Array of volumes of polar medium
GB1313121.4 2013-07-23
PCT/GB2013/052766 WO2014064443A2 (en) 2012-10-26 2013-10-23 Formation of array of membranes and apparatus therefor

Publications (2)

Publication Number Publication Date
AU2013336429A1 AU2013336429A1 (en) 2015-05-14
AU2013336429B2 true AU2013336429B2 (en) 2017-10-12

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US (7) US10814298B2 (enExample)
EP (2) EP3613499A1 (enExample)
JP (2) JP6495823B2 (enExample)
CN (2) CN104918696B (enExample)
AU (1) AU2013336429B2 (enExample)
CA (1) CA2889660C (enExample)
GB (1) GB201313121D0 (enExample)
WO (1) WO2014064443A2 (enExample)

Families Citing this family (168)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2122344B8 (en) 2007-02-20 2019-08-21 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
EP2391732B1 (en) 2009-01-30 2015-05-27 Oxford Nanopore Technologies Limited Methods using adaptors for nucleic acid constructs in transmembrane sequencing
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
SG10201604316WA (en) 2011-05-27 2016-07-28 Oxford Nanopore Tech Ltd Coupling method
KR20140050067A (ko) 2011-07-25 2014-04-28 옥스포드 나노포어 테크놀로지즈 리미티드 막횡단 포어를 사용한 이중 가닥 폴리뉴클레오티드 서열분석을 위한 헤어핀 루프 방법
EP3663412B1 (en) 2011-09-23 2022-03-09 Oxford Nanopore Technologies PLC Analysis of a polymer comprising polymer units by means of translocation through a nanopore
CN104039979B (zh) 2011-10-21 2016-08-24 牛津纳米孔技术公司 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
EP3736339B1 (en) 2012-02-16 2022-07-27 Oxford Nanopore Technologies plc Analysis of measurements of a polymer
WO2013153359A1 (en) 2012-04-10 2013-10-17 Oxford Nanopore Technologies Limited Mutant lysenin pores
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
AU2013291763B2 (en) 2012-07-19 2019-06-20 Oxford Nanopore Technologies Limited Modified helicases
JP6375301B2 (ja) * 2012-10-26 2018-08-15 オックスフォード ナノポール テクノロジーズ リミテッド 液滴界面
GB201313121D0 (en) * 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
CA2901545C (en) 2013-03-08 2019-10-08 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
EP3575410A3 (en) 2013-10-18 2020-03-04 Oxford Nanopore Technologies Limited Modified enzymes
EP3097210B2 (en) 2014-01-22 2022-04-20 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US10337060B2 (en) 2014-04-04 2019-07-02 Oxford Nanopore Technologies Ltd. Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
US10443097B2 (en) 2014-05-02 2019-10-15 Oxford Nanopore Technologies Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
CN119899249A (zh) 2014-09-01 2025-04-29 弗拉芒区生物技术研究所 突变csgg孔
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
EP3971300B1 (en) 2014-10-16 2024-06-05 Oxford Nanopore Technologies plc Sorting of polymers
GB201418512D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Electrical device with detachable components
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
CN107735686B (zh) 2015-04-14 2021-06-11 鲁汶天主教大学 具有内部蛋白质衔接子的纳米孔
GB201508669D0 (en) 2015-05-20 2015-07-01 Oxford Nanopore Tech Ltd Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown
CN109072295B (zh) 2015-12-08 2022-05-31 鲁汶大学研究与发展中心 修饰的纳米孔，包含其的组合物及其应用
US11333626B2 (en) 2016-02-22 2022-05-17 Hitachi, Ltd. Biological sample analysis chip, biological sample analyzer and biological sample analysis method
KR102222191B1 (ko) 2016-03-02 2021-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 기공
CN118272512A (zh) 2016-04-06 2024-07-02 牛津纳米孔科技公开有限公司 突变体孔
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
EP3464616B1 (en) 2016-05-25 2022-05-04 Oxford Nanopore Technologies plc Method
GB201611770D0 (en) 2016-07-06 2016-08-17 Oxford Nanopore Tech Microfluidic device
GB201616590D0 (en) 2016-09-29 2016-11-16 Oxford Nanopore Technologies Limited Method
JP2020504808A (ja) * 2016-10-26 2020-02-13 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ ナノポア配列決定用の集積回路およびフローセルのマルチチップパッケージング
GB201619930D0 (en) 2016-11-24 2017-01-11 Oxford Nanopore Tech Apparatus and methods for controlling insertion of a membrane channel into a membrane
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
RU2754808C2 (ru) 2016-12-22 2021-09-07 Иллюмина, Инк. Модуль проточной ячейки и способ его получения
CA3053086A1 (en) 2017-02-10 2018-08-16 Oxford Nanopore Technologies Limited Modified nanopores, compositions comprising the same, and uses thereof
JP2018155698A (ja) * 2017-03-21 2018-10-04 株式会社東芝 分析チップ
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB201707138D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Machine learning analysis of nanopore measurements
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
EP3642619B1 (en) 2017-06-20 2024-07-31 Illumina, Inc. Nanopore sequencing method
EP3645552B1 (en) 2017-06-30 2023-06-28 Vib Vzw Novel protein pores
AU2017434549B2 (en) 2017-09-28 2022-01-06 Oxford Nanopore Technologies Plc Kit of first and second parts adapted for connection to each other
US12011679B2 (en) * 2017-11-17 2024-06-18 International Business Machines Corporation Single layer nanofluidic separator chip and fluidic processor
GB2568895B (en) 2017-11-29 2021-10-27 Oxford Nanopore Tech Ltd Microfluidic device
GB2569630B (en) 2017-12-21 2022-10-12 Sharp Life Science Eu Ltd Droplet Interfaces in Electro-wetting Devices
GB201801768D0 (en) 2018-02-02 2018-03-21 Oxford Nanopore Tech Ltd Synthesis method
WO2019149626A1 (de) 2018-02-02 2019-08-08 Bayer Aktiengesellschaft Bekämpfung resistenter schadorganismen
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB2574048B (en) 2018-05-24 2021-06-16 Oxford Nanopore Tech Ltd Nanopore sensor component with electrostatic discharge protection
EP3801901A1 (en) 2018-05-24 2021-04-14 Oxford Nanopore Technologies Limited Droplet interfaces in electro-wetting devices
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
EP3807627A4 (en) 2018-06-13 2022-11-09 The New Zealand Institute for Plant and Food Research Limited BIOSENSOR DEVICE AND METHOD
US12698528B2 (en) 2018-07-30 2026-08-04 Oxford University Innovation Limited Assembly comprising a nucleic acid scaffold, systems, methods, and uses therefore
GB201814369D0 (en) 2018-09-04 2018-10-17 Oxford Nanopore Tech Ltd Method for determining a polymersequence
JP7499761B2 (ja) 2018-11-08 2024-06-14 オックスフォード ナノポール テクノロジーズ ピーエルシー 細孔
GB201818216D0 (en) 2018-11-08 2018-12-26 Oxford Nanopore Tech Ltd Pore
WO2020109800A1 (en) 2018-11-28 2020-06-04 Oxford Nanopore Technologies Ltd. Sensing system and method of operation
GB201819378D0 (en) 2018-11-28 2019-01-09 Oxford Nanopore Tech Ltd Analysis of nanopore signal using a machine-learning technique
GB201821155D0 (en) 2018-12-21 2019-02-06 Oxford Nanopore Tech Ltd Method
WO2020183172A1 (en) 2019-03-12 2020-09-17 Oxford Nanopore Technologies Inc. Nanopore sensing device and methods of operation and of forming it
GB2586339B (en) * 2019-03-19 2021-12-01 Oxford Nanopore Tech Ltd Current measurement apparatus, molecular entity sensing apparatus, method of measuring a current, method of sensing a molecular entity
GB2580988B (en) 2019-03-19 2022-04-13 Oxford Nanopore Tech Ltd Current measurement apparatus, molecular entity sensing apparatus, method of measuring a current, method of sensing a molecular entity
CN113677693B (zh) 2019-04-09 2025-10-21 牛津纳米孔科技公开有限公司 孔
GB201907243D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Sensing interactions between molecular entities and nanapores
GB201907246D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
GB201907244D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
GB201915480D0 (en) 2019-10-25 2019-12-11 Oxford Nanopore Tech Ltd Improved nanopore sensing device, components and method of manufacture
GB201917060D0 (en) 2019-11-22 2020-01-08 Oxford Nanopore Tech Ltd Method
WO2021111125A1 (en) 2019-12-02 2021-06-10 Oxford Nanopore Technologies Limited Method of characterising a target polypeptide using a nanopore
EP4071228A4 (en) 2019-12-03 2023-12-06 Japan Science and Technology Agency NANOPORE STRUCTURE AND BASE SEQUENCE ANALYSIS DEVICE COMPRISING A NANOPORE STRUCTURE
GB201917742D0 (en) 2019-12-04 2020-01-15 Oxford Nanopore Tech Ltd Method
GB201917832D0 (en) 2019-12-05 2020-01-22 Oxford Nanopore Tech Ltd Microfluidic device for preparing and analysing a test liquid
US10961563B1 (en) * 2019-12-19 2021-03-30 Robert Bosch Gmbh Nanoscale topography system for use in DNA sequencing and method for fabrication thereof
US20230047769A1 (en) * 2020-02-19 2023-02-16 Sony Semiconductor Solutions Corporation Biological substance detection chip, biological substance detection device and biological substance detection system
GB202004944D0 (en) 2020-04-03 2020-05-20 King S College London Method
GB202016874D0 (en) 2020-10-23 2020-12-09 Oxford Nanopore Tech Ltd Nanopore support structure and manufacture thereof
EP4168582B1 (en) 2020-06-18 2025-11-19 Oxford Nanopore Technologies plc A method of selectively characterising a polynucleotide using a detector
GB202009349D0 (en) 2020-06-18 2020-08-05 Oxford Nanopore Tech Ltd Method
US20240076729A9 (en) 2020-06-18 2024-03-07 Oxford Nanopore Technologies Plc Method
CN115989410A (zh) 2020-07-17 2023-04-18 牛津纳米孔科技公开有限公司 纳米孔感测装置
WO2022020461A1 (en) 2020-07-22 2022-01-27 Oxford Nanopore Technologies Inc. Solid state nanopore formation
CN116075596A (zh) 2020-08-07 2023-05-05 牛津纳米孔科技公开有限公司 鉴定核酸条形码的方法
GB202015993D0 (en) 2020-10-08 2020-11-25 Oxford Nanopore Tech Ltd Method
CN117255945A (zh) 2021-03-31 2023-12-19 伊鲁米纳公司 纳米孔传感器装置
EP4310487A4 (en) * 2021-04-30 2024-05-29 Qitan Technology Ltd., Chengdu PORE ARRAY LAYER STRUCTURE, CHIP DEVICE, NANOPORE SEQUENCING DEVICE, AND FILM FORMATION METHOD AND USE THEREOF
US20240240248A1 (en) 2021-05-19 2024-07-18 Oxford Nanopore Technologies Plc Methods for complement strand sequencing
GB202107192D0 (en) 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method
GB202107354D0 (en) 2021-05-24 2021-07-07 Oxford Nanopore Tech Ltd Method
CN113070113B (zh) * 2021-06-03 2021-08-20 成都齐碳科技有限公司 芯片结构、成膜方法、纳米孔测序装置及应用
CN113061531B (zh) * 2021-06-03 2021-08-20 成都齐碳科技有限公司 芯片结构、芯片组件、成膜方法、纳米孔测序装置及应用
CN113426499B (zh) * 2021-07-08 2022-10-14 成都齐碳科技有限公司 微结构、生物芯片、成膜方法、基因测序装置及其应用
EP4353835A4 (en) * 2021-08-31 2024-07-24 BGI Shenzhen MICROPOROUS STRUCTURE, MANUFACTURING PROCESS AND CHIP
EP4441744A1 (en) 2021-11-29 2024-10-09 Oxford Nanopore Technologies Ltd. Nanopore measurement signal analysis
WO2023116394A1 (zh) * 2021-12-21 2023-06-29 成都齐碳科技有限公司 成膜方法、包含膜的系统及应用
GB202118906D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
GB202118908D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
GB202118939D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Plc Pore
CN116930280A (zh) * 2022-04-07 2023-10-24 广州孔确基因科技有限公司 一种生成两亲性薄膜的方法及系统
GB202205617D0 (en) 2022-04-14 2022-06-01 Oxford Nanopore Tech Plc Novel modified protein pores and enzymes
CN114807982B (zh) * 2022-04-14 2024-01-16 广州孔确基因科技有限公司 一种两亲性分子层的制备方法及装置
CN114908358B8 (zh) * 2022-04-14 2024-06-21 孔确(成都)科技有限公司 一种两亲性分子层的制备方法及装置
CN119173641A (zh) 2022-05-17 2024-12-20 牛津纳米孔科技公开有限公司 方法和衔接子
GB202207267D0 (en) 2022-05-18 2022-06-29 Oxford Nanopore Tech Plc Calibration and profiling of a nanopore array device
US20230408439A1 (en) * 2022-06-14 2023-12-21 Illumina, Inc. Nanopore sensing systems
WO2023240427A1 (zh) * 2022-06-14 2023-12-21 成都齐碳科技有限公司 孔阵列层结构、预涂方法、成膜方法及测序装置
CN114751363B (zh) * 2022-06-14 2022-09-13 成都齐碳科技有限公司 孔阵列层结构、预涂方法、成膜方法及相关装置
GB202211602D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
CA3262948A1 (en) 2022-08-09 2024-02-15 Oxford Nanopore Technologies Plc NEW PORE-FORMING MONOMERS AND PORES
AU2023322679A1 (en) 2022-08-09 2025-01-23 Oxford Nanopore Technologies Plc De novo pores
GB202211607D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202215442D0 (en) 2022-10-19 2022-11-30 Oxford Nanopore Tech Plc Analysis of a polymer
CN120303290A (zh) 2022-10-28 2025-07-11 牛津纳米孔科技公开有限公司 孔单体和孔
GB202216162D0 (en) 2022-10-31 2022-12-14 Oxford Nanopore Tech Plc Method
CN120202306A (zh) 2022-11-01 2025-06-24 牛津纳米孔科技公开有限公司 生化分析系统以及控制生化分析系统的方法
CN120457488A (zh) 2022-11-10 2025-08-08 拜耳公司 基于遗传谱的靶向性作物保护产品施用
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202301095D0 (en) 2023-01-25 2023-03-08 Oxford Nanopore Tech Plc Calibration of a nanopore array device
GB202302813D0 (en) 2023-02-27 2023-04-12 Oxford Nanopore Tech Plc Nanopore sensor device
GB202302812D0 (en) 2023-02-27 2023-04-12 Oxford Nanopore Tech Plc Nanopore sensor device
GB202302811D0 (en) 2023-02-27 2023-04-12 Oxford Nanopore Tech Plc Nanopore sensor device
GB202302810D0 (en) 2023-02-27 2023-04-12 Oxford Nanopore Tech Plc Nanopore sensor device
GB202304324D0 (en) 2023-03-24 2023-05-10 Oxford Nanopore Tech Plc Method and kits
GB202305524D0 (en) 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
JP2026522806A (ja) * 2023-05-04 2026-07-09 エフ ホフマン－ラ ロッシュ アクチェン ゲゼルシャフト ハイブリッドトリブロックコポリマー膜組成物およびナノポア配列決定のための方法
GB202307141D0 (en) 2023-05-12 2023-06-28 Oxford Nanopore Tech Plc Modified helicases
GB202307494D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Ltd Nanopore sequencing and preparation modular instrument
GB202307486D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Plc Method
JPWO2025074983A1 (enExample) * 2023-10-02 2025-04-10
GB202315935D0 (en) 2023-10-18 2023-11-29 Oxford Nanopore Tech Ltd Novel enzymes
GB202317028D0 (en) 2023-11-06 2023-12-20 Oxford Nanopore Tech Ltd Method
GB202401864D0 (en) 2024-02-12 2024-03-27 Fund Centre De Regulacio Genòmica A method of detecting non-canonical bases in sequencing data
GB202405960D0 (en) 2024-04-29 2024-06-12 Oxford Nanopore Tech Plc Photo-active conjugates and their uses
GB202407099D0 (en) * 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc A flow cell and a method
GB202407044D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc Polymer analysis using transformer neural network
GB202407055D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc Enhanced polynucleotide sequencing
GB202407097D0 (en) * 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc A flow cell and a method
GB202407216D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Ltd A flow cell
GB202407247D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Plc Method
GB202407228D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Plc Method
WO2026052811A2 (en) 2024-09-06 2026-03-12 Oxford Nanopore Technologies Plc Method
WO2026052817A1 (en) 2024-09-06 2026-03-12 Oxford Nanopore Technologies Plc Method
GB202413506D0 (en) 2024-09-13 2024-10-30 Oxford Nanopore Tech Plc Pore monomers and their uses
WO2026057755A1 (en) 2024-09-13 2026-03-19 Oxford Nanopore Technologies Plc Adaptors and kits for rna molecules labelling and characterising
GB2644084A (en) 2024-09-16 2026-03-18 Oxford Nanopore Tech Plc Augmented consensus and variant calling
GB202413618D0 (en) 2024-09-16 2024-10-30 Oxford Nanopore Tech Plc peptide characterisation
GB202413747D0 (en) 2024-09-18 2024-10-30 Oxford Nanopore Tech Plc Preprocessing nanopore signals
GB2702789A (en) 2024-10-28 2026-07-01 Oxford Nanopore Tech Plc A nanopore array device and a method
WO2026120129A1 (en) 2024-12-06 2026-06-11 Oxford Nanopore Technologies plc. Pore constructs and their uses
GB202418735D0 (en) 2024-12-19 2025-02-05 Oxford Nanpore Tech Plc Methods and kits
GB202418836D0 (en) 2024-12-20 2025-02-05 Oxford Nanopore Tech Plc Method
WO2026154167A1 (en) 2025-01-17 2026-07-23 Oxford Nanopore Technologies Plc Method
GB202500688D0 (en) 2025-01-17 2025-03-05 Oxford Nanopore Tech Plc Pore monomers and their uses
WO2026162912A1 (en) 2025-01-31 2026-08-06 Oxford Nanopore Technologies Plc Methods and systems for polymer measurements

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4154795A (en) * 1976-07-23 1979-05-15 Dynatech Holdings Limited Microtest plates
US20100147450A1 (en) * 2005-07-29 2010-06-17 The University Of Tokyo Method of forming bilayer membrane by contact between amphipathic monolayers and apparatus therefor
US20100190253A1 (en) * 2007-06-18 2010-07-29 Kuraray Co., Ltd. Cell culture container and cell culture method
EP2219032A1 (en) * 2007-11-26 2010-08-18 The University of Tokyo Planar lipid-bilayer membrane array using microfluid and analysis method with the use of the planar lipid-bilayer membrane
US20110120871A1 (en) * 2007-12-19 2011-05-26 Stuart William Reid Formation of Layers of Amphiphilic Molecules
DE102010022929A1 (de) * 2010-06-07 2011-12-08 Albert-Ludwigs-Universität Freiburg Verfahren zum Herstellen einer Bilipidschicht sowie Mikrostruktur und Messanordnung

Family Cites Families (189)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3799743A (en) 1971-11-22 1974-03-26 Alexander James Stable lysis responsive lipid bilayer
JPS5274882A (en) 1975-12-18 1977-06-23 Fujitsu Ltd Superhigh density liquid contact connector
ES2150428T3 (es) 1987-04-27 2000-12-01 Unilever Nv Ensayos de union especifica.
US4874500A (en) 1987-07-15 1989-10-17 Sri International Microelectrochemical sensor and sensor array
DE68926118T2 (de) 1988-08-18 1996-08-22 Australian Membrane And Biotechnology Research Institute Ltd. Commonwealth Scientific And Industrial Research Organization, North Ryde, Neusuedwales Verbesserungen an der empfindlichkeit und der selektivität von ionenkanalmembranbiosensoren
GB8924338D0 (en) 1989-10-28 1989-12-13 Atomic Energy Authority Uk Electrodes
JPH0414773A (ja) 1990-05-07 1992-01-20 Fujitsu Ltd 電気的接続部材および装置
JPH04127066A (ja) 1990-09-18 1992-04-28 Fujitsu Ltd 信号端子接続方法および信号端子接続装置
JPH04215052A (ja) 1990-10-23 1992-08-05 Yokogawa Electric Corp 脂質膜型化学物質センサ
CA2077286C (en) 1991-09-10 1996-08-06 Tatsuo Chiyonobu Electrical connecting method
US5605662A (en) 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US5290240A (en) 1993-02-03 1994-03-01 Pharmetrix Corporation Electrochemical controlled dispensing assembly and method for selective and controlled delivery of a dispensing fluid
CA2157503A1 (en) 1993-03-05 1994-09-15 John M. Riviello Pulsed electrochemical detection using polymer electroactive electrodes
WO1994025862A1 (en) 1993-05-04 1994-11-10 Washington State University Research Foundation Biosensor substrate for mounting bilayer lipid membrane containing a receptor
US6095148A (en) 1995-11-03 2000-08-01 Children's Medical Center Corporation Neuronal stimulation using electrically conducting polymers
JP3822946B2 (ja) 1996-05-30 2006-09-20 三洋電機株式会社 二分子膜素子
JP3961588B2 (ja) 1996-06-18 2007-08-22 日本メジフィジックス株式会社 放射性核種溶出装置包装用内装材
EP0938674B1 (de) 1996-11-16 2005-06-01 NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen in Reutlingen Stiftung Bürgerlichen Rechts Mikroelementenanordnung, verfahren zum kontaktieren von in einer flüssigen umgebung befindlichen zellen und verfahren zum herstellen einer mikroelementenanordnung
US6503452B1 (en) 1996-11-29 2003-01-07 The Board Of Trustees Of The Leland Stanford Junior University Biosensor arrays and methods
US7169272B2 (en) 1997-04-30 2007-01-30 Board Of Trustees Of The University Of Arkansas Microfabricated recessed disk microelectrodes: characterization in static and convective solutions
US7144486B1 (en) 1997-04-30 2006-12-05 Board Of Trustees Of The University Of Arkansas Multilayer microcavity devices and methods
GB9712386D0 (en) 1997-06-14 1997-08-13 Univ Coventry Biosensor
US6300138B1 (en) 1997-08-01 2001-10-09 Qualigen, Inc. Methods for conducting tests
US6129823A (en) 1997-09-05 2000-10-10 Abbott Laboratories Low volume electrochemical sensor
US6483931B2 (en) 1997-09-11 2002-11-19 Stmicroelectronics, Inc. Electrostatic discharge protection of a capacitve type fingerprint sensing array
US20020144905A1 (en) 1997-12-17 2002-10-10 Christian Schmidt Sample positioning and analysis system
US7244349B2 (en) 1997-12-17 2007-07-17 Molecular Devices Corporation Multiaperture sample positioning and analysis system
ATE294240T1 (de) 1998-02-17 2005-05-15 Univ Cardiff Verfahren und kit, um biologische substanzen in plasmamembran und/oder zytosol einzuführen
CA2348002A1 (en) 1998-10-27 2000-05-04 Malcolm W. Mcgeoch Biological ion channels in nanofabricated detectors
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
WO2000052457A1 (en) 1999-03-02 2000-09-08 Helix Biopharma Corporation Card-based biosensor device
US7223364B1 (en) 1999-07-07 2007-05-29 3M Innovative Properties Company Detection article having fluid control film
US6916488B1 (en) 1999-11-05 2005-07-12 Biocure, Inc. Amphiphilic polymeric vesicles
WO2001059447A1 (en) 2000-02-11 2001-08-16 Yale University Planar patch clamp electrodes
WO2001083674A1 (en) 2000-05-03 2001-11-08 Gau Jen Jr Biological identification system with integrated sensor chip
AU2001287472A1 (en) 2000-09-19 2002-04-02 Cytion Sa Sample positioning and analysis system
CN100520407C (zh) 2000-10-02 2009-07-29 索菲昂生物科学有限公司 电生理测量系统
DE60026363T2 (de) 2000-10-25 2006-11-09 Xiamen University, Xiamen Elektroforetische trennungsvorrichtung und zugehöriges verwendungsverfahren
GB0026276D0 (en) 2000-10-27 2000-12-13 Univ Ulster Method for chlorine plasma modification of silver electrodes
CN1310379C (zh) 2001-01-16 2007-04-11 郑慧光 一种提高电线路便拆式连接的传导性能的方法
US6913697B2 (en) 2001-02-14 2005-07-05 Science & Technology Corporation @ Unm Nanostructured separation and analysis devices for biological membranes
AU2002338371A1 (en) 2001-04-06 2002-10-21 The Regents Of The University Of California Silicon-wafer based devices and methods for analyzing biological material
EP1388005B1 (en) * 2001-04-26 2006-11-15 Varian, Inc. Hollow fiber membrane sample preparation devices
US7077939B1 (en) 2001-06-18 2006-07-18 The Texas A&M University System Method and apparatus for nanoparticle transport and detection
JP2004534226A (ja) 2001-06-29 2004-11-11 メソ スケイル テクノロジーズ，エルエルシー 発光試験測定用のアッセイプレート、リーダシステム及び方法
US6863833B1 (en) 2001-06-29 2005-03-08 The Board Of Trustees Of The Leland Stanford Junior University Microfabricated apertures for supporting bilayer lipid membranes
US6890409B2 (en) 2001-08-24 2005-05-10 Applera Corporation Bubble-free and pressure-generating electrodes for electrophoretic and electroosmotic devices
AU2002365079A1 (en) 2001-10-03 2003-06-30 Osman A. Basaran Device and bioanalytical method utilizing asymmetric biofunction alized membrane
US20050230272A1 (en) 2001-10-03 2005-10-20 Lee Gil U Porous biosensing device
US7195872B2 (en) * 2001-11-09 2007-03-27 3D Biosurfaces, Inc. High surface area substrates for microarrays and methods to make same
US6783645B2 (en) 2001-12-18 2004-08-31 Dionex Corporation Disposable working electrode for an electrochemical cell
JP4127066B2 (ja) 2002-02-08 2008-07-30 東レ株式会社 易染色性ポリエステル繊維およびその製造方法
US20030224523A1 (en) 2002-05-30 2003-12-04 Thornberg John Herbert Cartridge arrangement, fluid analyzer arrangement, and methods
FR2844052B1 (fr) 2002-08-28 2005-07-01 Commissariat Energie Atomique Dispositif de mesure de l'activite electrique d'elements biologiques et ses applications
US8679517B2 (en) 2002-09-26 2014-03-25 Palmaz Scientific, Inc. Implantable materials having engineered surfaces made by vacuum deposition and method of making same
JP2004147534A (ja) * 2002-10-29 2004-05-27 Koji Nishimura 免疫調節作用関連の新規遺伝子及びＴｈ１ヘルパーＴ細胞とＴｈ２ヘルパーＴ細胞のバランスを調べる検査方法
JP2004158330A (ja) 2002-11-07 2004-06-03 Toshiba Corp 半導体装置のテストソケット
EP1419818B1 (de) 2002-11-14 2013-10-30 Boehringer Ingelheim microParts GmbH Vorrichtung zum schrittweisen Transport von Flüssigkeit unter Ausnutzung von Kapillarkräften
US20040115654A1 (en) * 2002-12-16 2004-06-17 Intel Corporation Laser exposure of photosensitive masks for DNA microarray fabrication
US20050003521A1 (en) * 2003-03-11 2005-01-06 O'connor David Addressable microarray device, methods of making, and uses thereof
CN1232813C (zh) 2003-03-13 2005-12-21 东南大学 制备纳米管探针针尖的方法
US7745116B2 (en) 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
JP3801617B2 (ja) 2003-06-27 2006-07-26 松下電器産業株式会社 薬理測定装置およびシステム並びにそれに用いるウェル容器
US7347921B2 (en) 2003-07-17 2008-03-25 Agilent Technologies, Inc. Apparatus and method for threading a biopolymer through a nanopore
JP4394916B2 (ja) 2003-09-19 2010-01-06 独立行政法人科学技術振興機構 人工脂質二重膜の形成装置および人工脂質二重膜の形成方法、並びにその利用
JP4394917B2 (ja) 2003-09-19 2010-01-06 独立行政法人科学技術振興機構 人工脂質二重膜を有する電流測定装置
JP3769622B2 (ja) 2003-09-22 2006-04-26 国立大学法人 東京大学 人工脂質膜の形成方法とそのための脂質平面膜形成装置
WO2005040783A1 (en) 2003-10-22 2005-05-06 Ambri Limited Novel sensor configuration
JP2005164276A (ja) 2003-11-28 2005-06-23 Sysmex Corp 分析装置および測定ユニット
US20050118061A1 (en) 2003-11-28 2005-06-02 Sysmex Corporation Analyzer, assay cartridge and analyzing method
KR100540143B1 (ko) 2003-12-22 2006-01-10 한국전자통신연구원 미소 유체 제어소자 및 미소 유체의 제어 방법
EP1548444A1 (en) 2003-12-23 2005-06-29 Paul Scherrer Institut An assay chip, and uses of said assay chip to determine molecular structures and functions
WO2005071405A1 (ja) 2004-01-21 2005-08-04 Japan Science And Technology Agency 膜タンパク質分析用平面脂質二重膜の形成方法とその装置
US8101431B2 (en) 2004-02-27 2012-01-24 Board Of Regents, The University Of Texas System Integration of fluids and reagents into self-contained cartridges containing sensor elements and reagent delivery systems
US20060257941A1 (en) 2004-02-27 2006-11-16 Mcdevitt John T Integration of fluids and reagents into self-contained cartridges containing particle and membrane sensor elements
JP2005300460A (ja) 2004-04-15 2005-10-27 Sony Corp 相互作用検出部と該検出部を備えるバイオアッセイ用基板、並びに媒質滴下方法と媒質の水分の蒸発を防止する方法
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
US20060292649A1 (en) 2004-07-19 2006-12-28 Cell Biosciences Inc. Methods and apparatus for reference lab diagnostics
GB2431013B (en) 2004-07-23 2008-05-21 Electronic Bio Sciences Llc Method and apparatus for sensing a time varying current passing through an ion channel
US7163830B2 (en) 2004-10-12 2007-01-16 Salmon Peter C Method for temporarily engaging electronic component for test
CA2594483C (en) 2005-01-28 2014-08-26 Duke University Apparatuses and methods for manipulating droplets on a printed circuit board
KR100698961B1 (ko) 2005-02-04 2007-03-26 주식회사 아이센스 전기화학적 바이오센서
AU2006212607B2 (en) 2005-02-10 2010-12-09 Koninklijke Philips Electronics N.V. Dual sample cartridge and method for characterizing particle in liquid
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
WO2006104639A2 (en) 2005-03-29 2006-10-05 Stanford University Device comprising array of micro-or nano-reservoirs
US20060228402A1 (en) 2005-04-08 2006-10-12 Charite-Universitatsmedizin Berlin Techniques for forming a lipid bilayer membrane
CA2605025A1 (en) 2005-04-15 2006-10-26 Genencor International, Inc. Viral nucleoprotein detection using an ion channel switch biosensor
JP4953044B2 (ja) 2005-05-09 2012-06-13 財団法人生産技術研究奨励会 脂質二重膜の形成方法およびその装置
JP2008544252A (ja) 2005-06-16 2008-12-04 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 神経変性疾患のための潜在的薬物分子の同定における、アミロイドベータタンパク質チャネルその、構造および使用
US8005526B2 (en) 2005-08-31 2011-08-23 The Regents Of The University Of Michigan Biologically integrated electrode devices
US8986781B2 (en) 2005-10-27 2015-03-24 Corning Incorporated Immobilized multi-layer artificial membrane for permeability measurements (PAMPA)
JP5161581B2 (ja) 2005-10-28 2013-03-13 株式会社クラレ 細胞培養容器及び細胞培養方法
WO2007093939A1 (en) 2006-02-13 2007-08-23 Koninklijke Philips Electronics N.V. Microfluidic device for molecular diagnostic applications
EP1998891B1 (en) 2006-03-20 2016-08-31 Koninklijke Philips N.V. A system-in-package platform for electronic-microfluidic devices
JP2009156572A (ja) 2006-04-06 2009-07-16 National Institutes Of Natural Sciences イオンチャンネルタンパク質バイオセンサー
US20070298511A1 (en) 2006-04-27 2007-12-27 The Texas A&M University System Nanopore sensor system
EP2041301B1 (en) 2006-05-17 2014-12-31 Eppendorf Array Technologies SA Identification and quantification of a plurality of biological (micro)organisms or their components
GB0614835D0 (en) 2006-07-26 2006-09-06 Isis Innovation Formation of bilayers of amphipathic molecules
WO2008054611A2 (en) 2006-10-04 2008-05-08 President And Fellows Of Harvard College Engineered conductive polymer films to mediate biochemical interactions
JP2008194573A (ja) 2007-02-09 2008-08-28 Matsushita Electric Ind Co Ltd 脂質二重膜形成方法
EP2122344B8 (en) 2007-02-20 2019-08-21 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
GB2446823A (en) 2007-02-20 2008-08-27 Oxford Nanolabs Ltd Formulation of lipid bilayers
US20080254995A1 (en) 2007-02-27 2008-10-16 Drexel University Nanopore arrays and sequencing devices and methods thereof
WO2008124107A1 (en) 2007-04-04 2008-10-16 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
CN100448007C (zh) 2007-04-19 2008-12-31 浙江大学 一种网格状静电放电防护器件
CN103495439B (zh) 2007-05-04 2015-09-16 欧普科诊断有限责任公司 流体连接器和微流体系统
CN100523799C (zh) 2007-06-27 2009-08-05 浙江大学 聚电解质/本征导电聚合物复合湿敏元件及其制作方法
GB0716264D0 (en) 2007-08-21 2007-09-26 Isis Innovation Bilayers
EP3540436B1 (en) 2007-09-12 2023-11-01 President And Fellows Of Harvard College High-resolution molecular sensor
CN101883865A (zh) * 2007-09-18 2010-11-10 普里莫里根生物科技有限责任公司 无框多重微阵列
WO2009073622A1 (en) 2007-11-30 2009-06-11 Electronic Bio Sciences, Llc Method and apparatus for single side bilayer formation
US8697007B2 (en) 2008-08-06 2014-04-15 The Trustees Of The University Of Pennsylvania Biodetection cassette with automated actuator
WO2010020912A1 (en) 2008-08-20 2010-02-25 Nxp B.V. Apparatus and method for molecule detection using nanopores
US20100190146A1 (en) 2009-01-29 2010-07-29 Bynum Magdalena A Microfluidic Glycan Analysis
CN102482330A (zh) 2009-01-30 2012-05-30 牛津纳米孔技术有限公司 酶突变体
JP2010186677A (ja) 2009-02-13 2010-08-26 Ritsumeikan 導電構造、アクチュエータ、可変抵抗器、回動部材、回動式コネクタ、電動機、コントローラ、回転情報検出装置、ストローク検出装置、および導電端子の製造方法
WO2010117470A2 (en) 2009-04-10 2010-10-14 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
CA2758617C (en) 2009-04-20 2017-10-03 Oxford Nanopore Technologies Limited Lipid bilayer sensor array
KR101399071B1 (ko) 2009-04-21 2014-05-27 존스테크 인터내셔널 코포레이션 마이크로회로 테스터용 전기 전도 켈빈 접점
GB0909923D0 (en) * 2009-06-09 2009-07-22 Oxford Gene Tech Ip Ltd Picowell capture devices for analysing single cells or other particles
US8174279B2 (en) 2009-08-21 2012-05-08 Freescale Semiconductor, Inc. Socket connector for connection lead of semiconductor device under test with tester
AU2010307229B2 (en) 2009-09-18 2016-02-25 President And Fellows Of Harvard College Bare single-layer graphene membrane having a nanopore enabling high-sensitivity molecular detection and analysis
EP2826564B1 (en) * 2009-11-23 2018-12-26 3M Innovative Properties Company Microwell array articles and methods of use
EP2507387B1 (en) 2009-12-01 2017-01-25 Oxford Nanopore Technologies Limited Biochemical analysis instrument and method
US20120010085A1 (en) 2010-01-19 2012-01-12 Rava Richard P Methods for determining fraction of fetal nucleic acids in maternal samples
US20110287414A1 (en) 2010-02-08 2011-11-24 Genia Technologies, Inc. Systems and methods for identifying a portion of a molecule
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
KR20110100963A (ko) 2010-03-05 2011-09-15 삼성전자주식회사 미세 유동 장치 및 이를 이용한 표적 핵산의 염기 서열 결정 방법
KR101832004B1 (ko) 2010-03-23 2018-02-23 주식회사 쿠라레 다능성 포유 세포를 분화시키는 배양 방법
EP2614156B1 (en) 2010-09-07 2018-08-01 The Regents of The University of California Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme
WO2012042226A2 (en) 2010-10-01 2012-04-05 Oxford Nanopore Technologies Limited Biochemical analysis apparatus and rotary valve
BR112013020411B1 (pt) 2011-02-11 2021-09-08 Oxford Nanopore Technologies Limited Monômero de msp mutante, construto, polinucleotídeo, poro, kit e aparelho para caracterizar uma sequência de ácido nucleico alvo, e, método para caracterizar uma sequência de ácido nucleico alvo
WO2012138357A1 (en) 2011-04-04 2012-10-11 President And Fellows Of Harvard College Nanopore sensing by local electrical potential measurement
JP2012247231A (ja) 2011-05-25 2012-12-13 Fujikura Kasei Co Ltd 検査器具
CN102263104B (zh) 2011-06-16 2013-04-17 北京大学 Mos结构的esd保护器件
AU2012284137C1 (en) 2011-07-20 2017-02-02 The Regents Of The University Of California Dual-pore device
US10761043B2 (en) 2011-07-22 2020-09-01 The Trustees Of The University Of Pennsylvania Graphene-based nanopore and nanostructure devices and methods for macromolecular analysis
WO2013016486A1 (en) 2011-07-27 2013-01-31 The Board Of Trustees Of The University Of Illinois Nanopore sensors for biomolecular characterization
JP5670278B2 (ja) 2011-08-09 2015-02-18 株式会社日立ハイテクノロジーズ ナノポア式分析装置
EP3663412B1 (en) 2011-09-23 2022-03-09 Oxford Nanopore Technologies PLC Analysis of a polymer comprising polymer units by means of translocation through a nanopore
CN104039979B (zh) 2011-10-21 2016-08-24 牛津纳米孔技术公司 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法
US9696277B2 (en) 2011-11-14 2017-07-04 The Regents Of The University Of California Two-chamber dual-pore device
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
CA2861808C (en) 2011-12-29 2021-02-23 Oxford Nanopore Technologies Limited Enzyme method
JP5898969B2 (ja) 2012-01-18 2016-04-06 株式会社日立製作所 半導体装置
JP5912582B2 (ja) 2012-01-27 2016-04-27 ローム株式会社 包材入り液体試薬内蔵型マイクロチップおよびその使用方法
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
US8541849B2 (en) 2012-02-14 2013-09-24 Genia Technologies, Inc. Noise shielding techniques for ultra low current measurements in biochemical applications
EP3736339B1 (en) 2012-02-16 2022-07-27 Oxford Nanopore Technologies plc Analysis of measurements of a polymer
WO2013153359A1 (en) 2012-04-10 2013-10-17 Oxford Nanopore Technologies Limited Mutant lysenin pores
US9758821B2 (en) 2012-04-17 2017-09-12 International Business Machines Corporation Graphene transistor gated by charges through a nanopore for bio-molecular sensing and DNA sequencing
JP5692164B2 (ja) 2012-05-22 2015-04-01 ウシオ電機株式会社 マイクロチップへの試薬供給方法及びマイクロチップへの試薬供給装置
JP6172711B2 (ja) 2012-07-05 2017-08-02 国立研究開発法人理化学研究所 マイクロチップ用の流体制御デバイスおよびその利用
CA2879355C (en) 2012-07-19 2021-09-21 Oxford Nanopore Technologies Limited Helicase construct and its use in characterising polynucleotides
AU2013291763B2 (en) 2012-07-19 2019-06-20 Oxford Nanopore Technologies Limited Modified helicases
WO2014019603A1 (de) 2012-07-30 2014-02-06 Nmi Naturwissenschaftliches Und Medizinisches Institut An Der Universitaet Tuebingen Anschlussplatte für einen mikrofuidischen probenchip, mikrofluidischer probenchip und untersuchungsverfahren mit einem mikrofluidischen probenanordnungsbereich
WO2014052616A2 (en) 2012-09-27 2014-04-03 The Trustees Of The University Of Pennsylvania Insulated nanoelectrode-nanopore devices and related methods
JP6375301B2 (ja) 2012-10-26 2018-08-15 オックスフォード ナノポール テクノロジーズ リミテッド 液滴界面
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
WO2014070235A1 (en) 2012-10-29 2014-05-08 Mbio Diagnostics, Inc. Biological particle identification system, cartridge and associated methods
JPWO2014132343A1 (ja) 2013-02-26 2017-02-02 株式会社日立製作所 Ｆｅｔアレイ基板、分析システム、及び方法
RU2669844C2 (ru) 2013-03-14 2018-10-16 Аркема Инк. Способы сшивания полимерных композиций в присутствии атмосферного кислорода
JP2014190891A (ja) 2013-03-28 2014-10-06 Hitachi High-Technologies Corp ナノポア式分析装置の電圧印加システム
GB2515104B (en) 2013-06-14 2015-07-22 Suunto Oy A device and a method for assembling an electronic device and a flexible element for facilitating assembly of electronic components
US9279801B2 (en) 2013-07-26 2016-03-08 Axion Biosystems, Inc. Devices, systems and methods for high-throughput electrophysiology
CN203466320U (zh) 2013-09-20 2014-03-05 番禺得意精密电子工业有限公司 电连接器
EP3575410A3 (en) 2013-10-18 2020-03-04 Oxford Nanopore Technologies Limited Modified enzymes
US9828623B2 (en) 2014-03-13 2017-11-28 Illinois Institute Of Technology Nanopore stochastic sensing of biomarkers
JP6960223B2 (ja) 2014-05-27 2021-11-05 イラミーナ インコーポレーテッド 基礎器具と取外可能カートリッジとを含む生化学的解析のためのシステム及び方法
CN103995035A (zh) 2014-05-29 2014-08-20 东南大学 用于碱基序列检测的多栅极石墨烯场效应管结构及制备方法
CN106457247B (zh) 2014-06-16 2019-09-03 皇家飞利浦有限公司 用于快速样品摄取的盒
GB201418512D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Electrical device with detachable components
CN105701440B (zh) 2014-12-11 2019-01-15 义隆电子股份有限公司 具静电防护的指纹感测器
CN105701441B (zh) 2014-12-15 2018-10-09 义隆电子股份有限公司 指纹感测器的感测方法及电路
US9658190B2 (en) 2014-12-18 2017-05-23 Genia Technologies, Inc. Printed electrode
US10794895B2 (en) 2015-02-05 2020-10-06 President And Fellows Of Harvard College Nanopore sensor including fluidic passage
AU2016253147B2 (en) 2015-04-24 2021-07-22 Mesa Biotech, Inc. Fluidic test cassette
GB201508669D0 (en) 2015-05-20 2015-07-01 Oxford Nanopore Tech Ltd Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown
WO2017061600A1 (ja) 2015-10-08 2017-04-13 凸版印刷株式会社 マイクロ流体デバイスおよび試料分析方法
GB201611770D0 (en) 2016-07-06 2016-08-17 Oxford Nanopore Tech Microfluidic device
CN205828393U (zh) 2016-07-15 2016-12-21 中芯国际集成电路制造(北京)有限公司 一种用于静电放电保护的高密度栅极二极管
JP7038147B2 (ja) 2017-06-21 2022-03-17 ヌーマ バイオ インコーポレイテッド 二重細孔制御およびセンサデバイス
AU2017434549B2 (en) 2017-09-28 2022-01-06 Oxford Nanopore Technologies Plc Kit of first and second parts adapted for connection to each other
GB2568895B (en) 2017-11-29 2021-10-27 Oxford Nanopore Tech Ltd Microfluidic device
WO2019160925A1 (en) 2018-02-16 2019-08-22 Illumina, Inc. Device for sequencing
GB2574048B (en) 2018-05-24 2021-06-16 Oxford Nanopore Tech Ltd Nanopore sensor component with electrostatic discharge protection
WO2020183172A1 (en) 2019-03-12 2020-09-17 Oxford Nanopore Technologies Inc. Nanopore sensing device and methods of operation and of forming it
CN115989410A (zh) 2020-07-17 2023-04-18 牛津纳米孔科技公开有限公司 纳米孔感测装置
US11940404B2 (en) 2022-02-16 2024-03-26 Western Digital Technologies, Inc. Low noise amplifiers with shields for nanopore Applications

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4154795A (en) * 1976-07-23 1979-05-15 Dynatech Holdings Limited Microtest plates
US20100147450A1 (en) * 2005-07-29 2010-06-17 The University Of Tokyo Method of forming bilayer membrane by contact between amphipathic monolayers and apparatus therefor
US20100190253A1 (en) * 2007-06-18 2010-07-29 Kuraray Co., Ltd. Cell culture container and cell culture method
EP2219032A1 (en) * 2007-11-26 2010-08-18 The University of Tokyo Planar lipid-bilayer membrane array using microfluid and analysis method with the use of the planar lipid-bilayer membrane
US20110120871A1 (en) * 2007-12-19 2011-05-26 Stuart William Reid Formation of Layers of Amphiphilic Molecules
DE102010022929A1 (de) * 2010-06-07 2011-12-08 Albert-Ludwigs-Universität Freiburg Verfahren zum Herstellen einer Bilipidschicht sowie Mikrostruktur und Messanordnung

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