AU2013336429B2 - Formation of array of membranes and apparatus therefor - Google Patents

Formation of array of membranes and apparatus therefor Download PDF

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Publication number
AU2013336429B2
AU2013336429B2 AU2013336429A AU2013336429A AU2013336429B2 AU 2013336429 B2 AU2013336429 B2 AU 2013336429B2 AU 2013336429 A AU2013336429 A AU 2013336429A AU 2013336429 A AU2013336429 A AU 2013336429A AU 2013336429 B2 AU2013336429 B2 AU 2013336429B2
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medium
polar medium
volumes
polar
support
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AU2013336429A1 (en
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Pedro Miguel Ortiz BAHAMON
Clive Gavin Brown
Andrew John Heron
Jason Robert Hyde
Paul Raymond MACKETT
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Oxford Nanopore Technologies PLC
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Oxford Nanopore Technologies PLC
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5088—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above confining liquids at a location by surface tension, e.g. virtual wells on plates, wires
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B01J2219/00317—Microwell devices, i.e. having large numbers of wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00734—Lipids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00736—Non-biologic macromolecules, e.g. polymeric compounds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0642—Filling fluids into wells by specific techniques
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/974—Thrombin

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Clinical Laboratory Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
AU2013336429A 2012-10-26 2013-10-23 Formation of array of membranes and apparatus therefor Active AU2013336429B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261718899P 2012-10-26 2012-10-26
US61/718,899 2012-10-26
GB1313121.4 2013-07-23
GBGB1313121.4A GB201313121D0 (en) 2013-07-23 2013-07-23 Array of volumes of polar medium
PCT/GB2013/052766 WO2014064443A2 (en) 2012-10-26 2013-10-23 Formation of array of membranes and apparatus therefor

Publications (2)

Publication Number Publication Date
AU2013336429A1 AU2013336429A1 (en) 2015-05-14
AU2013336429B2 true AU2013336429B2 (en) 2017-10-12

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US (6) US10814298B2 (enExample)
EP (2) EP3613499A1 (enExample)
JP (2) JP6495823B2 (enExample)
CN (2) CN109569458B (enExample)
AU (1) AU2013336429B2 (enExample)
CA (1) CA2889660C (enExample)
GB (1) GB201313121D0 (enExample)
WO (1) WO2014064443A2 (enExample)

Families Citing this family (148)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ579083A (en) 2007-02-20 2012-07-27 Oxford Nanopore Tech Ltd Lipid bilayer sensor system
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
JP5843614B2 (ja) 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
AU2012264497B2 (en) 2011-05-27 2017-06-15 Oxford Nanopore Technologies Limited Coupling method
JP6298404B2 (ja) 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
WO2013041878A1 (en) 2011-09-23 2013-03-28 Oxford Nanopore Technologies Limited Analysis of a polymer comprising polymer units
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
EP3736339B1 (en) 2012-02-16 2022-07-27 Oxford Nanopore Technologies plc Analysis of measurements of a polymer
CN112646019B (zh) 2012-04-10 2022-08-16 牛津纳米孔科技公开有限公司 突变胞溶素孔
AU2013291763B2 (en) 2012-07-19 2019-06-20 Oxford Nanopore Technologies Limited Modified helicases
EP2875154B1 (en) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB method for characterising a nucleic acid
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
US9823235B2 (en) * 2012-10-26 2017-11-21 Oxford Nanopre Technologies Ltd. Droplet interfaces
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
AU2014224432B2 (en) 2013-03-08 2019-10-24 Oxford Nanopore Technologies Limited Enzyme stalling method
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
CA2927726A1 (en) 2013-10-18 2015-04-23 Oxford Nanopore Technologies Limited Modified helicases for use in nucleic acid sequencing
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
EP3097210B2 (en) 2014-01-22 2022-04-20 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
EP3126515B1 (en) 2014-04-04 2018-08-29 Oxford Nanopore Technologies Limited Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
EP3137490B1 (en) 2014-05-02 2021-01-27 Oxford Nanopore Technologies Limited Mutant pores
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
CN115851894B (zh) 2014-10-16 2025-06-27 牛津楠路珀尔科技股份有限公司 聚合物的分析
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
GB201418512D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Electrical device with detachable components
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
EP3283887B1 (en) 2015-04-14 2021-07-21 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
GB201508669D0 (en) 2015-05-20 2015-07-01 Oxford Nanopore Tech Ltd Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown
AU2016369071B2 (en) 2015-12-08 2022-05-19 Katholieke Universiteit Leuven Ku Leuven Research & Development Modified nanopores, compositions comprising the same, and uses thereof
US11333626B2 (en) 2016-02-22 2022-05-17 Hitachi, Ltd. Biological sample analysis chip, biological sample analyzer and biological sample analysis method
KR102222192B1 (ko) 2016-03-02 2021-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 기공
JP7364333B2 (ja) 2016-04-06 2023-10-18 オックスフォード ナノポール テクノロジーズ ピーエルシー 変異体ポア
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
WO2017203268A1 (en) 2016-05-25 2017-11-30 Oxford Nanopore Technologies Limited Method
GB201611770D0 (en) 2016-07-06 2016-08-17 Oxford Nanopore Tech Microfluidic device
GB201616590D0 (en) 2016-09-29 2016-11-16 Oxford Nanopore Technologies Limited Method
JP2020504808A (ja) 2016-10-26 2020-02-13 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ ナノポア配列決定用の集積回路およびフローセルのマルチチップパッケージング
GB201619930D0 (en) 2016-11-24 2017-01-11 Oxford Nanopore Tech Apparatus and methods for controlling insertion of a membrane channel into a membrane
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
JP7236999B2 (ja) 2016-12-22 2023-03-10 イラミーナ インコーポレーテッド フローセルパッケージおよびその製造方法
AU2018218531B2 (en) 2017-02-10 2021-01-28 Oxford Nanopore Technologies Limited Modified nanopores, compositions comprising the same, and uses thereof
JP2018155698A (ja) * 2017-03-21 2018-10-04 株式会社東芝 分析チップ
GB201707138D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Machine learning analysis of nanopore measurements
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
AU2018288767B2 (en) 2017-06-20 2022-05-26 Illumina, Inc. Nanopore sequencers
CN117106037B (zh) 2017-06-30 2025-07-25 弗拉芒区生物技术研究所 新颖蛋白孔
WO2019063959A1 (en) 2017-09-28 2019-04-04 Oxford Nanopore Technologies Limited KIT OF FIRST AND SECOND PARTS DESIGNED TO BE CONNECTED TO ONE ANOTHER
US12011679B2 (en) * 2017-11-17 2024-06-18 International Business Machines Corporation Single layer nanofluidic separator chip and fluidic processor
GB2568895B (en) 2017-11-29 2021-10-27 Oxford Nanopore Tech Ltd Microfluidic device
GB2569630B (en) 2017-12-21 2022-10-12 Sharp Life Science Eu Ltd Droplet Interfaces in Electro-wetting Devices
GB201801768D0 (en) 2018-02-02 2018-03-21 Oxford Nanopore Tech Ltd Synthesis method
WO2019149626A1 (de) 2018-02-02 2019-08-08 Bayer Aktiengesellschaft Bekämpfung resistenter schadorganismen
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB2574048B (en) 2018-05-24 2021-06-16 Oxford Nanopore Tech Ltd Nanopore sensor component with electrostatic discharge protection
CN112203767B (zh) 2018-05-24 2023-04-11 牛津纳米孔科技公司 电润湿装置中的液滴界面
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
GB201814369D0 (en) 2018-09-04 2018-10-17 Oxford Nanopore Tech Ltd Method for determining a polymersequence
JP7499761B2 (ja) 2018-11-08 2024-06-14 オックスフォード ナノポール テクノロジーズ ピーエルシー 細孔
GB201818216D0 (en) 2018-11-08 2018-12-26 Oxford Nanopore Tech Ltd Pore
WO2020109800A1 (en) 2018-11-28 2020-06-04 Oxford Nanopore Technologies Ltd. Sensing system and method of operation
GB201819378D0 (en) 2018-11-28 2019-01-09 Oxford Nanopore Tech Ltd Analysis of nanopore signal using a machine-learning technique
GB201821155D0 (en) 2018-12-21 2019-02-06 Oxford Nanopore Tech Ltd Method
KR20210138594A (ko) 2019-03-12 2021-11-19 옥스포드 나노포어 테크놀로지즈 피엘씨 나노포어 감지 디바이스 및 이를 작동하는 방법 및 형성하는 방법
GB2586339B (en) * 2019-03-19 2021-12-01 Oxford Nanopore Tech Ltd Current measurement apparatus, molecular entity sensing apparatus, method of measuring a current, method of sensing a molecular entity
GB2580988B (en) 2019-03-19 2022-04-13 Oxford Nanopore Tech Ltd Current measurement apparatus, molecular entity sensing apparatus, method of measuring a current, method of sensing a molecular entity
CA3134796A1 (en) 2019-04-09 2020-10-15 Oxford Nanopore Technologies Limited Pore
GB201907244D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
GB201907246D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
GB201907243D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Sensing interactions between molecular entities and nanapores
GB201915480D0 (en) * 2019-10-25 2019-12-11 Oxford Nanopore Tech Ltd Improved nanopore sensing device, components and method of manufacture
GB201917060D0 (en) 2019-11-22 2020-01-08 Oxford Nanopore Tech Ltd Method
CN118011004A (zh) 2019-12-02 2024-05-10 牛津纳米孔科技公开有限公司 使用纳米孔表征靶多肽的方法
WO2021111987A1 (ja) 2019-12-03 2021-06-10 国立研究開発法人科学技術振興機構 ナノポア構造体、ナノポア構造体を含む塩基配列解析装置
GB201917742D0 (en) 2019-12-04 2020-01-15 Oxford Nanopore Tech Ltd Method
GB201917832D0 (en) 2019-12-05 2020-01-22 Oxford Nanopore Tech Ltd Microfluidic device for preparing and analysing a test liquid
US10961563B1 (en) * 2019-12-19 2021-03-30 Robert Bosch Gmbh Nanoscale topography system for use in DNA sequencing and method for fabrication thereof
JPWO2021166597A1 (enExample) * 2020-02-19 2021-08-26
GB202004944D0 (en) 2020-04-03 2020-05-20 King S College London Method
GB202016874D0 (en) * 2020-10-23 2020-12-09 Oxford Nanopore Tech Ltd Nanopore support structure and manufacture thereof
CN115698331A (zh) 2020-06-18 2023-02-03 牛津纳米孔科技公开有限公司 使用检测器选择性地表征多核苷酸的方法
GB202009349D0 (en) 2020-06-18 2020-08-05 Oxford Nanopore Tech Ltd Method
EP4168583A2 (en) 2020-06-18 2023-04-26 Oxford Nanopore Technologies plc Method of characterising a polynucleotide moving through a nanopore
WO2022013551A1 (en) 2020-07-17 2022-01-20 Oxford Nanopore Technologies Limited Nanopore sensing device
WO2022020461A1 (en) 2020-07-22 2022-01-27 Oxford Nanopore Technologies Inc. Solid state nanopore formation
US20220059187A1 (en) 2020-08-07 2022-02-24 Oxford Nanopore Technologies Limited Methods of detecting nucleic acid barcodes
GB202015993D0 (en) 2020-10-08 2020-11-25 Oxford Nanopore Tech Ltd Method
WO2022227080A1 (zh) * 2021-04-30 2022-11-03 成都齐碳科技有限公司 孔阵列层结构、芯片装置、纳米孔测序装置和成膜方法以及用途
CN117337333A (zh) 2021-05-19 2024-01-02 牛津纳米孔科技公开有限公司 用于补体链测序的方法
GB202107192D0 (en) 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method
GB202107354D0 (en) 2021-05-24 2021-07-07 Oxford Nanopore Tech Ltd Method
CN113070113B (zh) * 2021-06-03 2021-08-20 成都齐碳科技有限公司 芯片结构、成膜方法、纳米孔测序装置及应用
CN113061531B (zh) * 2021-06-03 2021-08-20 成都齐碳科技有限公司 芯片结构、芯片组件、成膜方法、纳米孔测序装置及应用
CN113426499B (zh) * 2021-07-08 2022-10-14 成都齐碳科技有限公司 微结构、生物芯片、成膜方法、基因测序装置及其应用
US20250006308A1 (en) 2021-11-29 2025-01-02 Oxford Nanopore Technologies Plc Nanopore measurement signal analysis
CN116297721A (zh) * 2021-12-21 2023-06-23 成都齐碳科技有限公司 成膜方法、包含膜的系统及应用
GB202118939D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Plc Pore
GB202118908D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
GB202118906D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
GB202205617D0 (en) 2022-04-14 2022-06-01 Oxford Nanopore Tech Plc Novel modified protein pores and enzymes
CN114908358B8 (zh) * 2022-04-14 2024-06-21 孔确(成都)科技有限公司 一种两亲性分子层的制备方法及装置
CN117779058A (zh) * 2022-04-14 2024-03-29 广州孔确基因科技有限公司 一种两亲性分子层的制备方法及装置
WO2023222657A1 (en) 2022-05-17 2023-11-23 Oxford Nanopore Technologies Plc Method and adaptors
GB202207267D0 (en) 2022-05-18 2022-06-29 Oxford Nanopore Tech Plc Calibration and profiling of a nanopore array device
CN114751363B (zh) * 2022-06-14 2022-09-13 成都齐碳科技有限公司 孔阵列层结构、预涂方法、成膜方法及相关装置
WO2023240427A1 (zh) * 2022-06-14 2023-12-21 成都齐碳科技有限公司 孔阵列层结构、预涂方法、成膜方法及测序装置
KR20250044691A (ko) 2022-08-09 2025-04-01 옥스포드 나노포어 테크놀로지즈 피엘씨 신규 포어 단량체 및 포어
GB202211607D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202211602D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
KR20250048551A (ko) 2022-08-09 2025-04-09 옥스포드 나노포어 테크놀로지즈 피엘씨 신규 포어
GB202215442D0 (en) 2022-10-19 2022-11-30 Oxford Nanopore Tech Plc Analysis of a polymer
EP4608850A2 (en) 2022-10-28 2025-09-03 Oxford Nanopore Technologies PLC Pore monomers and pores
GB202216162D0 (en) 2022-10-31 2022-12-14 Oxford Nanopore Tech Plc Method
WO2024094966A1 (en) 2022-11-01 2024-05-10 Oxford Nanopore Technologies Plc Biochemical analysis system and method of controlling a biochemical analysis system
WO2024099985A1 (en) 2022-11-10 2024-05-16 Bayer Aktiengesellschaft Targeted crop protection product application based on genetic profiles
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202301095D0 (en) 2023-01-25 2023-03-08 Oxford Nanopore Tech Plc Calibration of a nanopore array device
GB202302812D0 (en) 2023-02-27 2023-04-12 Oxford Nanopore Tech Plc Nanopore sensor device
GB202302810D0 (en) 2023-02-27 2023-04-12 Oxford Nanopore Tech Plc Nanopore sensor device
GB202302811D0 (en) 2023-02-27 2023-04-12 Oxford Nanopore Tech Plc Nanopore sensor device
GB202302813D0 (en) 2023-02-27 2023-04-12 Oxford Nanopore Tech Plc Nanopore sensor device
GB202304324D0 (en) 2023-03-24 2023-05-10 Oxford Nanopore Tech Plc Method and kits
GB202305524D0 (en) 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
WO2024227837A1 (en) * 2023-05-04 2024-11-07 F. Hoffmann-La Roche Ag Hybrid triblock copolymer membrane compositions and methods for nanopore sequencing
GB202307141D0 (en) 2023-05-12 2023-06-28 Oxford Nanopore Tech Plc Modified helicases
GB202307494D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Ltd Nanopore sequencing and preparation modular instrument
GB202307486D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Plc Method
WO2025074983A1 (ja) * 2023-10-02 2025-04-10 地方独立行政法人神奈川県立産業技術総合研究所 脂質二重膜形成用隔壁並びにそれを含む脂質二重膜形成デバイス及びそれを用いた脂質二重膜形成方法
GB202315935D0 (en) 2023-10-18 2023-11-29 Oxford Nanopore Tech Ltd Novel enzymes
GB202317028D0 (en) 2023-11-06 2023-12-20 Oxford Nanopore Tech Ltd Method
GB202401864D0 (en) 2024-02-12 2024-03-27 Fund Centre De Regulacio Genòmica A method of detecting non-canonical bases in sequencing data
GB202405960D0 (en) 2024-04-29 2024-06-12 Oxford Nanopore Tech Plc Photo-active conjugates and their uses
GB202407044D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc Polymer analysis using transformer neural network
GB202407097D0 (en) * 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc A flow cell and a method
GB202407055D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc Enhanced polynucleotide sequencing
GB202407099D0 (en) * 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc A flow cell and a method
GB202407216D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Ltd A flow cell
GB202407228D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Plc Method
GB202407247D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Plc Method

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4154795A (en) * 1976-07-23 1979-05-15 Dynatech Holdings Limited Microtest plates
US20100147450A1 (en) * 2005-07-29 2010-06-17 The University Of Tokyo Method of forming bilayer membrane by contact between amphipathic monolayers and apparatus therefor
US20100190253A1 (en) * 2007-06-18 2010-07-29 Kuraray Co., Ltd. Cell culture container and cell culture method
EP2219032A1 (en) * 2007-11-26 2010-08-18 The University of Tokyo Planar lipid-bilayer membrane array using microfluid and analysis method with the use of the planar lipid-bilayer membrane
US20110120871A1 (en) * 2007-12-19 2011-05-26 Stuart William Reid Formation of Layers of Amphiphilic Molecules
DE102010022929A1 (de) * 2010-06-07 2011-12-08 Albert-Ludwigs-Universität Freiburg Verfahren zum Herstellen einer Bilipidschicht sowie Mikrostruktur und Messanordnung

Family Cites Families (188)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3799743A (en) 1971-11-22 1974-03-26 Alexander James Stable lysis responsive lipid bilayer
JPS5274882A (en) 1975-12-18 1977-06-23 Fujitsu Ltd Superhigh density liquid contact connector
ES2150428T3 (es) 1987-04-27 2000-12-01 Unilever Nv Ensayos de union especifica.
US4874500A (en) 1987-07-15 1989-10-17 Sri International Microelectrochemical sensor and sensor array
DE68926118T2 (de) 1988-08-18 1996-08-22 Australian Membrane And Biotechnology Research Institute Ltd. Commonwealth Scientific And Industrial Research Organization, North Ryde, Neusuedwales Verbesserungen an der empfindlichkeit und der selektivität von ionenkanalmembranbiosensoren
GB8924338D0 (en) 1989-10-28 1989-12-13 Atomic Energy Authority Uk Electrodes
JPH0414773A (ja) 1990-05-07 1992-01-20 Fujitsu Ltd 電気的接続部材および装置
JPH04127066A (ja) 1990-09-18 1992-04-28 Fujitsu Ltd 信号端子接続方法および信号端子接続装置
JPH04215052A (ja) 1990-10-23 1992-08-05 Yokogawa Electric Corp 脂質膜型化学物質センサ
CA2077286C (en) 1991-09-10 1996-08-06 Tatsuo Chiyonobu Electrical connecting method
US5605662A (en) 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US5290240A (en) 1993-02-03 1994-03-01 Pharmetrix Corporation Electrochemical controlled dispensing assembly and method for selective and controlled delivery of a dispensing fluid
CA2157503A1 (en) 1993-03-05 1994-09-15 John M. Riviello Pulsed electrochemical detection using polymer electroactive electrodes
WO1994025862A1 (en) 1993-05-04 1994-11-10 Washington State University Research Foundation Biosensor substrate for mounting bilayer lipid membrane containing a receptor
US6095148A (en) 1995-11-03 2000-08-01 Children's Medical Center Corporation Neuronal stimulation using electrically conducting polymers
JP3822946B2 (ja) 1996-05-30 2006-09-20 三洋電機株式会社 二分子膜素子
JP3961588B2 (ja) 1996-06-18 2007-08-22 日本メジフィジックス株式会社 放射性核種溶出装置包装用内装材
EP0938674B1 (de) 1996-11-16 2005-06-01 NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen in Reutlingen Stiftung Bürgerlichen Rechts Mikroelementenanordnung, verfahren zum kontaktieren von in einer flüssigen umgebung befindlichen zellen und verfahren zum herstellen einer mikroelementenanordnung
US6503452B1 (en) 1996-11-29 2003-01-07 The Board Of Trustees Of The Leland Stanford Junior University Biosensor arrays and methods
US7169272B2 (en) 1997-04-30 2007-01-30 Board Of Trustees Of The University Of Arkansas Microfabricated recessed disk microelectrodes: characterization in static and convective solutions
US7144486B1 (en) 1997-04-30 2006-12-05 Board Of Trustees Of The University Of Arkansas Multilayer microcavity devices and methods
GB9712386D0 (en) 1997-06-14 1997-08-13 Univ Coventry Biosensor
US6300138B1 (en) 1997-08-01 2001-10-09 Qualigen, Inc. Methods for conducting tests
US6129823A (en) 1997-09-05 2000-10-10 Abbott Laboratories Low volume electrochemical sensor
US6483931B2 (en) 1997-09-11 2002-11-19 Stmicroelectronics, Inc. Electrostatic discharge protection of a capacitve type fingerprint sensing array
US7244349B2 (en) 1997-12-17 2007-07-17 Molecular Devices Corporation Multiaperture sample positioning and analysis system
US20020144905A1 (en) 1997-12-17 2002-10-10 Christian Schmidt Sample positioning and analysis system
WO1999041401A1 (en) 1998-02-17 1999-08-19 University Of Wales College Of Medicine Method and apparatus for introducing substances into the cell plasma membrane and/or cytosol
CA2348002A1 (en) 1998-10-27 2000-05-04 Malcolm W. Mcgeoch Biological ion channels in nanofabricated detectors
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
AU5646800A (en) 1999-03-02 2000-09-21 Helix Biopharma Corporation Card-based biosensor device
US7223364B1 (en) 1999-07-07 2007-05-29 3M Innovative Properties Company Detection article having fluid control film
US6916488B1 (en) 1999-11-05 2005-07-12 Biocure, Inc. Amphiphilic polymeric vesicles
AU2001234996A1 (en) 2000-02-11 2001-08-20 Yale University Planar patch clamp electrodes
CA2407973C (en) 2000-05-03 2011-06-07 Jen-Jr Gau Biological identification system with integrated sensor chip
WO2002024862A2 (en) 2000-09-19 2002-03-28 Cytion S.A. Sample positioning and analysis system
AU2001293676B2 (en) 2000-10-02 2006-07-27 Sophion Bioscience A/S System for electrophysiological measurements
EP1328798B1 (en) 2000-10-25 2006-03-01 Xiamen University Electrophoretic separating device and method for using the device
GB0026276D0 (en) 2000-10-27 2000-12-13 Univ Ulster Method for chlorine plasma modification of silver electrodes
CN1310379C (zh) 2001-01-16 2007-04-11 郑慧光 一种提高电线路便拆式连接的传导性能的方法
US6913697B2 (en) 2001-02-14 2005-07-05 Science & Technology Corporation @ Unm Nanostructured separation and analysis devices for biological membranes
AU2002338371A1 (en) 2001-04-06 2002-10-21 The Regents Of The University Of California Silicon-wafer based devices and methods for analyzing biological material
DE60216076T2 (de) * 2001-04-26 2007-06-21 Varian, Inc., Palo Alto Hohlfasermembran probenpräparationsanordnungen
US7077939B1 (en) 2001-06-18 2006-07-18 The Texas A&M University System Method and apparatus for nanoparticle transport and detection
US6863833B1 (en) 2001-06-29 2005-03-08 The Board Of Trustees Of The Leland Stanford Junior University Microfabricated apertures for supporting bilayer lipid membranes
DK1412725T3 (en) 2001-06-29 2019-03-25 Meso Scale Technologies Llc Multi-well plates for LUMINESCENSE TEST MEASUREMENTS
US6890409B2 (en) 2001-08-24 2005-05-10 Applera Corporation Bubble-free and pressure-generating electrodes for electrophoretic and electroosmotic devices
US20050230272A1 (en) 2001-10-03 2005-10-20 Lee Gil U Porous biosensing device
US7374944B2 (en) 2001-10-03 2008-05-20 Purdue Research Foundation Device and bioanalytical method utilizing asymmetric biofunctionalized membrane
ATE509272T1 (de) * 2001-11-09 2011-05-15 3Dbiosurfaces Technologies Llc Substrate mit hochliegendem oberflächenbereich für mikroarrays sowie verfahren zur herstellung davon
US6783645B2 (en) 2001-12-18 2004-08-31 Dionex Corporation Disposable working electrode for an electrochemical cell
JP4127066B2 (ja) 2002-02-08 2008-07-30 東レ株式会社 易染色性ポリエステル繊維およびその製造方法
US20030224523A1 (en) 2002-05-30 2003-12-04 Thornberg John Herbert Cartridge arrangement, fluid analyzer arrangement, and methods
FR2844052B1 (fr) 2002-08-28 2005-07-01 Commissariat Energie Atomique Dispositif de mesure de l'activite electrique d'elements biologiques et ses applications
US8679517B2 (en) 2002-09-26 2014-03-25 Palmaz Scientific, Inc. Implantable materials having engineered surfaces made by vacuum deposition and method of making same
JP2004147534A (ja) * 2002-10-29 2004-05-27 Koji Nishimura 免疫調節作用関連の新規遺伝子及びＴｈ１ヘルパーＴ細胞とＴｈ２ヘルパーＴ細胞のバランスを調べる検査方法
JP2004158330A (ja) 2002-11-07 2004-06-03 Toshiba Corp 半導体装置のテストソケット
EP1419818B1 (de) 2002-11-14 2013-10-30 Boehringer Ingelheim microParts GmbH Vorrichtung zum schrittweisen Transport von Flüssigkeit unter Ausnutzung von Kapillarkräften
US20040115654A1 (en) * 2002-12-16 2004-06-17 Intel Corporation Laser exposure of photosensitive masks for DNA microarray fabrication
CN1232813C (zh) 2003-03-13 2005-12-21 东南大学 制备纳米管探针针尖的方法
US7745116B2 (en) 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
JP3801617B2 (ja) 2003-06-27 2006-07-26 松下電器産業株式会社 薬理測定装置およびシステム並びにそれに用いるウェル容器
US7347921B2 (en) 2003-07-17 2008-03-25 Agilent Technologies, Inc. Apparatus and method for threading a biopolymer through a nanopore
JP4394917B2 (ja) 2003-09-19 2010-01-06 独立行政法人科学技術振興機構 人工脂質二重膜を有する電流測定装置
JP4394916B2 (ja) 2003-09-19 2010-01-06 独立行政法人科学技術振興機構 人工脂質二重膜の形成装置および人工脂質二重膜の形成方法、並びにその利用
JP3769622B2 (ja) 2003-09-22 2006-04-26 国立大学法人 東京大学 人工脂質膜の形成方法とそのための脂質平面膜形成装置
EP1678488A1 (en) 2003-10-22 2006-07-12 Ambri Limited Novel sensor configuration
EP1535667A1 (en) 2003-11-28 2005-06-01 Sysmex Corporation Analyzer, assay cartridge and analyzing method
JP2005164276A (ja) 2003-11-28 2005-06-23 Sysmex Corp 分析装置および測定ユニット
KR100540143B1 (ko) 2003-12-22 2006-01-10 한국전자통신연구원 미소 유체 제어소자 및 미소 유체의 제어 방법
EP1548444A1 (en) 2003-12-23 2005-06-29 Paul Scherrer Institut An assay chip, and uses of said assay chip to determine molecular structures and functions
US8039247B2 (en) 2004-01-21 2011-10-18 Japan Science And Technology Agency Method of forming planar lipid double membrane for membrane protein analysis and apparatus therefor
US8101431B2 (en) 2004-02-27 2012-01-24 Board Of Regents, The University Of Texas System Integration of fluids and reagents into self-contained cartridges containing sensor elements and reagent delivery systems
US20060257941A1 (en) 2004-02-27 2006-11-16 Mcdevitt John T Integration of fluids and reagents into self-contained cartridges containing particle and membrane sensor elements
JP2005300460A (ja) 2004-04-15 2005-10-27 Sony Corp 相互作用検出部と該検出部を備えるバイオアッセイ用基板、並びに媒質滴下方法と媒質の水分の蒸発を防止する方法
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
US20060292649A1 (en) 2004-07-19 2006-12-28 Cell Biosciences Inc. Methods and apparatus for reference lab diagnostics
GB2431013B (en) 2004-07-23 2008-05-21 Electronic Bio Sciences Llc Method and apparatus for sensing a time varying current passing through an ion channel
US7163830B2 (en) 2004-10-12 2007-01-16 Salmon Peter C Method for temporarily engaging electronic component for test
PL1859330T3 (pl) 2005-01-28 2013-01-31 Univ Duke Urządzenia i sposoby manipulacji kropelkami na płytkach obwodów drukowanych
KR100698961B1 (ko) 2005-02-04 2007-03-26 주식회사 아이센스 전기화학적 바이오센서
CA2597496A1 (en) 2005-02-10 2006-08-17 Chempaq A/S Dual sample cartridge and method for characterizing particles in liquid
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
WO2006104639A2 (en) 2005-03-29 2006-10-05 Stanford University Device comprising array of micro-or nano-reservoirs
US20060228402A1 (en) 2005-04-08 2006-10-12 Charite-Universitatsmedizin Berlin Techniques for forming a lipid bilayer membrane
WO2006113550A2 (en) 2005-04-15 2006-10-26 Genencor International, Inc. Viral nucleoprotein detection using an ion channel switch biosensor
JP4953044B2 (ja) 2005-05-09 2012-06-13 財団法人生産技術研究奨励会 脂質二重膜の形成方法およびその装置
US20070238184A1 (en) 2005-06-16 2007-10-11 The Regents Of The University Of California Amyloid beta protein channel structure and uses thereof in identifying potential drug molecules for neurodegenerative diseases
US8005526B2 (en) 2005-08-31 2011-08-23 The Regents Of The University Of Michigan Biologically integrated electrode devices
US8986781B2 (en) 2005-10-27 2015-03-24 Corning Incorporated Immobilized multi-layer artificial membrane for permeability measurements (PAMPA)
WO2007049576A1 (ja) 2005-10-28 2007-05-03 Kuraray Co., Ltd. 細胞培養容器及び細胞培養方法
EP1987275A1 (en) 2006-02-13 2008-11-05 Koninklijke Philips Electronics N.V. Microfluidic device for molecular diagnostic applications
RU2422204C2 (ru) 2006-03-20 2011-06-27 Конинклейке Филипс Электроникс Н.В. Платформа система в корпусе для электронно-микрофлюидных устройств
JP2009156572A (ja) 2006-04-06 2009-07-16 National Institutes Of Natural Sciences イオンチャンネルタンパク質バイオセンサー
US20070298511A1 (en) 2006-04-27 2007-12-27 The Texas A&M University System Nanopore sensor system
AU2007251545A1 (en) 2006-05-17 2007-11-22 Eppendorf Array Technologies S.A. Identification and quantification of a plurality of biological (micro)organisms or their components
GB0614835D0 (en) 2006-07-26 2006-09-06 Isis Innovation Formation of bilayers of amphipathic molecules
US9018019B2 (en) 2006-10-04 2015-04-28 President And Fellows Of Harvard College Engineered conductive polymer films to mediate biochemical interactions
JP2008194573A (ja) 2007-02-09 2008-08-28 Matsushita Electric Ind Co Ltd 脂質二重膜形成方法
GB2446823A (en) 2007-02-20 2008-08-27 Oxford Nanolabs Ltd Formulation of lipid bilayers
NZ579083A (en) 2007-02-20 2012-07-27 Oxford Nanopore Tech Ltd Lipid bilayer sensor system
US20080254995A1 (en) 2007-02-27 2008-10-16 Drexel University Nanopore arrays and sequencing devices and methods thereof
AU2008236694B2 (en) 2007-04-04 2014-01-23 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
CN100448007C (zh) 2007-04-19 2008-12-31 浙江大学 一种网格状静电放电防护器件
CN103495439B (zh) 2007-05-04 2015-09-16 欧普科诊断有限责任公司 流体连接器和微流体系统
CN100523799C (zh) 2007-06-27 2009-08-05 浙江大学 聚电解质/本征导电聚合物复合湿敏元件及其制作方法
GB0716264D0 (en) 2007-08-21 2007-09-26 Isis Innovation Bilayers
WO2009035647A1 (en) 2007-09-12 2009-03-19 President And Fellows Of Harvard College High-resolution molecular graphene sensor comprising an aperture in the graphene layer
WO2009039286A2 (en) * 2007-09-18 2009-03-26 Primorigen Biosciences, Llc Frameless multiplexed microarrays
WO2009073622A1 (en) 2007-11-30 2009-06-11 Electronic Bio Sciences, Llc Method and apparatus for single side bilayer formation
US8697007B2 (en) 2008-08-06 2014-04-15 The Trustees Of The University Of Pennsylvania Biodetection cassette with automated actuator
US8669124B2 (en) 2008-08-20 2014-03-11 Nxp, B.V. Apparatus and method for molecule detection using nanopores
US20100190146A1 (en) 2009-01-29 2010-07-29 Bynum Magdalena A Microfluidic Glycan Analysis
CA2750874A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Hybridization linkers
JP2010186677A (ja) 2009-02-13 2010-08-26 Ritsumeikan 導電構造、アクチュエータ、可変抵抗器、回動部材、回動式コネクタ、電動機、コントローラ、回転情報検出装置、ストローク検出装置、および導電端子の製造方法
US8986928B2 (en) 2009-04-10 2015-03-24 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
JP5781502B2 (ja) 2009-04-20 2015-09-24 オックスフォード ナノポール テクノロジーズ リミテッド 脂質二重層センサアレイ
US8558554B2 (en) 2009-04-21 2013-10-15 Johnstech International Corporation Electrically conductive Kelvin contacts for microcircuit tester
GB0909923D0 (en) 2009-06-09 2009-07-22 Oxford Gene Tech Ip Ltd Picowell capture devices for analysing single cells or other particles
US8174279B2 (en) 2009-08-21 2012-05-08 Freescale Semiconductor, Inc. Socket connector for connection lead of semiconductor device under test with tester
EP3196645B1 (en) 2009-09-18 2019-06-19 President and Fellows of Harvard College Bare single-layer graphene membrane having a nanopore enabling high-sensitivity molecular detection and analysis
WO2011063332A2 (en) * 2009-11-23 2011-05-26 3M Innovative Properties Company Microwell array articles and methods of use
BR112012013074B1 (pt) 2009-12-01 2018-09-18 Oxford Nanopore Technologies Limited instrumento de análise e módulo para realizar análise bioquímica, e, método para operar um instrumento de análise para realizar análise bioquímica
WO2011090556A1 (en) 2010-01-19 2011-07-28 Verinata Health, Inc. Methods for determining fraction of fetal nucleic acid in maternal samples
US20110287414A1 (en) 2010-02-08 2011-11-24 Genia Technologies, Inc. Systems and methods for identifying a portion of a molecule
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
KR20110100963A (ko) 2010-03-05 2011-09-15 삼성전자주식회사 미세 유동 장치 및 이를 이용한 표적 핵산의 염기 서열 결정 방법
JP6159529B2 (ja) * 2010-03-23 2017-07-05 株式会社クラレ 多能性哺乳細胞を分化させる培養方法
WO2012033524A2 (en) 2010-09-07 2012-03-15 The Regents Of The University Of California Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme
CN103370617B (zh) 2010-10-01 2015-11-25 牛津纳米孔技术有限公司 生物化学分析设备和旋转阀
KR101939420B1 (ko) 2011-02-11 2019-01-16 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 세공
EP2694671B1 (en) 2011-04-04 2021-01-06 President and Fellows of Harvard College Nanopore sensor comprising a device for measuring the local electrical potential
JP2012247231A (ja) 2011-05-25 2012-12-13 Fujikura Kasei Co Ltd 検査器具
CN102263104B (zh) 2011-06-16 2013-04-17 北京大学 Mos结构的esd保护器件
CA2944478A1 (en) 2011-07-20 2013-01-24 The Regents Of The University Of California Dual-pore device
US10761043B2 (en) 2011-07-22 2020-09-01 The Trustees Of The University Of Pennsylvania Graphene-based nanopore and nanostructure devices and methods for macromolecular analysis
JP2014521956A (ja) 2011-07-27 2014-08-28 ザ ボード オブ トラスティーズ オブ ザ ユニヴァーシティー オブ イリノイ 生体分子を特徴付けるためのナノ細孔センサー
JP5670278B2 (ja) 2011-08-09 2015-02-18 株式会社日立ハイテクノロジーズ ナノポア式分析装置
WO2013041878A1 (en) 2011-09-23 2013-03-28 Oxford Nanopore Technologies Limited Analysis of a polymer comprising polymer units
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
US9696277B2 (en) 2011-11-14 2017-07-04 The Regents Of The University Of California Two-chamber dual-pore device
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
KR102086182B1 (ko) 2011-12-29 2020-03-06 옥스포드 나노포어 테크놀로지즈 리미티드 효소 방법
JP5898969B2 (ja) 2012-01-18 2016-04-06 株式会社日立製作所 半導体装置
JP5912582B2 (ja) 2012-01-27 2016-04-27 ローム株式会社 包材入り液体試薬内蔵型マイクロチップおよびその使用方法
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
US8541849B2 (en) 2012-02-14 2013-09-24 Genia Technologies, Inc. Noise shielding techniques for ultra low current measurements in biochemical applications
EP3736339B1 (en) 2012-02-16 2022-07-27 Oxford Nanopore Technologies plc Analysis of measurements of a polymer
CN112646019B (zh) 2012-04-10 2022-08-16 牛津纳米孔科技公开有限公司 突变胞溶素孔
US9758821B2 (en) 2012-04-17 2017-09-12 International Business Machines Corporation Graphene transistor gated by charges through a nanopore for bio-molecular sensing and DNA sequencing
JP5692164B2 (ja) 2012-05-22 2015-04-01 ウシオ電機株式会社 マイクロチップへの試薬供給方法及びマイクロチップへの試薬供給装置
JP6172711B2 (ja) 2012-07-05 2017-08-02 国立研究開発法人理化学研究所 マイクロチップ用の流体制御デバイスおよびその利用
AU2013291763B2 (en) 2012-07-19 2019-06-20 Oxford Nanopore Technologies Limited Modified helicases
US9797009B2 (en) 2012-07-19 2017-10-24 Oxford Nanopore Technologies Limited Enzyme construct
EP3584010A1 (de) 2012-07-30 2019-12-25 NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen Untersuchungsverfahren an und/oder mit einem biologischen probenmaterial
US10876157B2 (en) 2012-09-27 2020-12-29 The Trustees Of The University Of Pennsylvania Insulated nanoelectrode-nanopore devices and related methods
US9823235B2 (en) 2012-10-26 2017-11-21 Oxford Nanopre Technologies Ltd. Droplet interfaces
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
EP2911791A4 (en) 2012-10-29 2016-11-02 Mbio Diagnostics Inc BIOPARTICLE IDENTIFICATION SYSTEM, CARTRIDGE AND RELATED METHODS
WO2014132343A1 (ja) 2013-02-26 2014-09-04 株式会社日立製作所 Ｆｅｔアレイ基板、分析システム、及び方法
EP4039733A1 (en) 2013-03-14 2022-08-10 Arkema, Inc. Methods for crosslinking polymer compositions in the presence of atmospheric oxygen
JP2014190891A (ja) 2013-03-28 2014-10-06 Hitachi High-Technologies Corp ナノポア式分析装置の電圧印加システム
GB2515104B (en) 2013-06-14 2015-07-22 Suunto Oy A device and a method for assembling an electronic device and a flexible element for facilitating assembly of electronic components
US9279801B2 (en) 2013-07-26 2016-03-08 Axion Biosystems, Inc. Devices, systems and methods for high-throughput electrophysiology
CN203466320U (zh) 2013-09-20 2014-03-05 番禺得意精密电子工业有限公司 电连接器
CA2927726A1 (en) 2013-10-18 2015-04-23 Oxford Nanopore Technologies Limited Modified helicases for use in nucleic acid sequencing
US9828623B2 (en) 2014-03-13 2017-11-28 Illinois Institute Of Technology Nanopore stochastic sensing of biomarkers
WO2015183871A1 (en) 2014-05-27 2015-12-03 Illumina, Inc. Systems and methods for biochemical analysis including a base instrument and a removable cartridge
CN103995035A (zh) 2014-05-29 2014-08-20 东南大学 用于碱基序列检测的多栅极石墨烯场效应管结构及制备方法
JP6360568B2 (ja) 2014-06-16 2018-07-18 コーニンクレッカ フィリップス エヌ ヴェＫｏｎｉｎｋｌｉｊｋｅ Ｐｈｉｌｉｐｓ Ｎ．Ｖ． 迅速な試料取り込みのためのカートリッジ
GB201418512D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Electrical device with detachable components
CN105701440B (zh) 2014-12-11 2019-01-15 义隆电子股份有限公司 具静电防护的指纹感测器
CN105701441B (zh) 2014-12-15 2018-10-09 义隆电子股份有限公司 指纹感测器的感测方法及电路
US9658190B2 (en) 2014-12-18 2017-05-23 Genia Technologies, Inc. Printed electrode
US10794895B2 (en) 2015-02-05 2020-10-06 President And Fellows Of Harvard College Nanopore sensor including fluidic passage
JP6830443B2 (ja) 2015-04-24 2021-02-17 メサ バイオテック，インク． 流体検査用カセット
GB201508669D0 (en) 2015-05-20 2015-07-01 Oxford Nanopore Tech Ltd Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown
JPWO2017061600A1 (ja) 2015-10-08 2018-08-02 凸版印刷株式会社 マイクロ流体デバイスおよび試料分析方法
GB201611770D0 (en) 2016-07-06 2016-08-17 Oxford Nanopore Tech Microfluidic device
CN205828393U (zh) 2016-07-15 2016-12-21 中芯国际集成电路制造(北京)有限公司 一种用于静电放电保护的高密度栅极二极管
CA3067993C (en) 2017-06-21 2024-02-27 Ontera Inc. Dual pore-control and sensor device
WO2019063959A1 (en) 2017-09-28 2019-04-04 Oxford Nanopore Technologies Limited KIT OF FIRST AND SECOND PARTS DESIGNED TO BE CONNECTED TO ONE ANOTHER
GB2568895B (en) 2017-11-29 2021-10-27 Oxford Nanopore Tech Ltd Microfluidic device
CA3066469C (en) 2018-02-16 2022-06-21 Illumina, Inc. Device for sequencing
GB2574048B (en) 2018-05-24 2021-06-16 Oxford Nanopore Tech Ltd Nanopore sensor component with electrostatic discharge protection
KR20210138594A (ko) 2019-03-12 2021-11-19 옥스포드 나노포어 테크놀로지즈 피엘씨 나노포어 감지 디바이스 및 이를 작동하는 방법 및 형성하는 방법
WO2022013551A1 (en) 2020-07-17 2022-01-20 Oxford Nanopore Technologies Limited Nanopore sensing device
US11940404B2 (en) 2022-02-16 2024-03-26 Western Digital Technologies, Inc. Low noise amplifiers with shields for nanopore Applications

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4154795A (en) * 1976-07-23 1979-05-15 Dynatech Holdings Limited Microtest plates
US20100147450A1 (en) * 2005-07-29 2010-06-17 The University Of Tokyo Method of forming bilayer membrane by contact between amphipathic monolayers and apparatus therefor
US20100190253A1 (en) * 2007-06-18 2010-07-29 Kuraray Co., Ltd. Cell culture container and cell culture method
EP2219032A1 (en) * 2007-11-26 2010-08-18 The University of Tokyo Planar lipid-bilayer membrane array using microfluid and analysis method with the use of the planar lipid-bilayer membrane
US20110120871A1 (en) * 2007-12-19 2011-05-26 Stuart William Reid Formation of Layers of Amphiphilic Molecules
DE102010022929A1 (de) * 2010-06-07 2011-12-08 Albert-Ludwigs-Universität Freiburg Verfahren zum Herstellen einer Bilipidschicht sowie Mikrostruktur und Messanordnung

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