CA2866590A1 - Defined media for expansion and maintenance of pluripotent stem cells - Google Patents

Defined media for expansion and maintenance of pluripotent stem cells Download PDF

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Publication number
CA2866590A1
CA2866590A1 CA2866590A CA2866590A CA2866590A1 CA 2866590 A1 CA2866590 A1 CA 2866590A1 CA 2866590 A CA2866590 A CA 2866590A CA 2866590 A CA2866590 A CA 2866590A CA 2866590 A1 CA2866590 A1 CA 2866590A1
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Prior art keywords
cell culture
cells
media
culture formulation
defined cell
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Abandoned
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CA2866590A
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English (en)
French (fr)
Inventor
Alireza Rezania
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Janssen Biotech Inc
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Janssen Biotech Inc
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Filing date
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Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of CA2866590A1 publication Critical patent/CA2866590A1/en
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/24Iron; Fe chelators; Transferrin
    • C12N2500/25Insulin-transferrin; Insulin-transferrin-selenium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/34Sugars
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/36Lipids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/38Vitamins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/105Insulin-like growth factors [IGF]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/115Basic fibroblast growth factor (bFGF, FGF-2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/15Transforming growth factor beta (TGF-β)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Reproductive Health (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2866590A 2012-03-07 2013-03-06 Defined media for expansion and maintenance of pluripotent stem cells Abandoned CA2866590A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261607706P 2012-03-07 2012-03-07
US61/607,706 2012-03-07
PCT/US2013/029360 WO2013134378A1 (en) 2012-03-07 2013-03-06 Defined media for expansion and maintenance of pluripotent stem cells

Publications (1)

Publication Number Publication Date
CA2866590A1 true CA2866590A1 (en) 2013-09-12

Family

ID=49114468

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2866590A Abandoned CA2866590A1 (en) 2012-03-07 2013-03-06 Defined media for expansion and maintenance of pluripotent stem cells

Country Status (16)

Country Link
US (3) US9434920B2 (enExample)
EP (1) EP2823037A4 (enExample)
JP (1) JP6383292B2 (enExample)
KR (1) KR20140131999A (enExample)
CN (1) CN104160018A (enExample)
AR (1) AR090276A1 (enExample)
AU (2) AU2013230020B2 (enExample)
CA (1) CA2866590A1 (enExample)
HK (1) HK1206058A1 (enExample)
IN (1) IN2014DN07036A (enExample)
MX (1) MX354775B (enExample)
PH (1) PH12014501898A1 (enExample)
RU (2) RU2664467C2 (enExample)
SG (1) SG11201405052RA (enExample)
WO (1) WO2013134378A1 (enExample)
ZA (1) ZA201407241B (enExample)

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CN103088065B (zh) * 2013-01-25 2014-12-10 北京银杏德济生物技术有限公司 一种能够大规模并快速高纯度地诱导间充质干细胞转决定成造血干细胞的方法
WO2014192938A1 (ja) 2013-05-30 2014-12-04 味の素株式会社 幹細胞の培養用培地
WO2015066631A2 (en) * 2013-11-01 2015-05-07 University Of Notre Dame Du Lac Cell culture medium and bioprocess optimization
AU2015243936B2 (en) * 2014-04-07 2021-08-05 Memorial Sloan Kettering Cancer Center Modulating cell proliferation and pluripotency
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US10597634B2 (en) * 2014-12-31 2020-03-24 Peking University School And Hospital Of Stomatology Device and use thereof in cell experiments in vitro
DK3430129T5 (da) * 2016-03-16 2024-09-16 Cynata Therapeutics Ltd Kolonidannende medium og anvendelse deraf
KR102015815B1 (ko) * 2016-08-10 2019-08-29 가톨릭대학교 산학협력단 유도만능줄기세포의 분화를 유도하여 각막 상피 세포를 배양하는 방법 및 시스템
WO2018115953A1 (en) * 2016-12-21 2018-06-28 Prometic Pharma Smt Limited Methods and compositions for preventing or minimizing epithelial-mesenchymal transition
JP7049347B2 (ja) 2017-01-23 2022-04-06 ステムセル テクノロジーズ カナダ インコーポレイテッド 幹細胞の生存および増殖を高めるための培地および方法
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AU2018333272B2 (en) * 2017-09-15 2022-12-08 Cynata Therapeutics Limited Method for treating allergic airways disease (AAD)/ asthma
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AU2020283056B2 (en) 2019-05-31 2023-06-08 Viacyte, Inc. A biocompatible membrane composite
AU2020282355B2 (en) 2019-05-31 2023-11-02 Viacyte, Inc. A biocompatible membrane composite
CA3139292C (en) 2019-05-31 2025-06-10 Viacyte, Inc. CELL ENCAPSULATION DEVICES WITH REGULATED OXYGEN DIFFUSION DISTANCES
WO2020243666A1 (en) 2019-05-31 2020-12-03 W. L. Gore & Associates, Inc. A biocompatible membrane composite
CN112516291B (zh) * 2019-09-17 2023-07-14 通化安睿特生物制药股份有限公司 含人白蛋白的制剂及其制备方法
US11001810B1 (en) * 2019-11-11 2021-05-11 Lancell AB Serum-free human pluripotent stem cell culture medium
CN112708591B (zh) * 2020-12-24 2022-04-29 南京周子未来食品科技有限公司 一种用于肌肉干细胞体外分化的化学成分明确的培养基
CN115141798A (zh) * 2022-07-18 2022-10-04 上海食未生物科技有限公司 一种无血清培养基及其在培养肌肉干细胞中的用途和方法

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