MX354775B - Medios definidos para la expansion y el mantenimiento de celulas madre pluripotentes. - Google Patents

Medios definidos para la expansion y el mantenimiento de celulas madre pluripotentes.

Info

Publication number
MX354775B
MX354775B MX2014010782A MX2014010782A MX354775B MX 354775 B MX354775 B MX 354775B MX 2014010782 A MX2014010782 A MX 2014010782A MX 2014010782 A MX2014010782 A MX 2014010782A MX 354775 B MX354775 B MX 354775B
Authority
MX
Mexico
Prior art keywords
stem cells
pluripotent stem
maintenance
expansion
defined media
Prior art date
Application number
MX2014010782A
Other languages
English (en)
Other versions
MX2014010782A (es
Inventor
Alireza Rezania
Original Assignee
Janssen Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of MX2014010782A publication Critical patent/MX2014010782A/es
Publication of MX354775B publication Critical patent/MX354775B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Reproductive Health (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención se refiere a un método para la expansión de células madre pluripotentes humanas, caracterizado porque comprende cultivar las células madre pluripotentes humanas en una matriz libre de alimentador en una formulación de cultivo celular definido para expander de ese modo las células, en donde la formulación de cultivo celular definido consiste esencialmente de medio basal DMEM-F12, insulina, transferrina, selenio, albúmina libre de ácidos grasos, de aproximadamente 5 ng/ml a aproximadamente 10 ng/ml de ligando TGF-ß de aproximadamente 50 ng/ml a aproximadamente 100 ng/ml de bFGF, factor de crecimiento de insulina tipo 1 (IGF.1) y ácido ascórbico, en donde el cultivo de las células madre pluripotentes humanas en la formulación de cultivo celular definido mantiene la pluripotencia y la estabilidad cariotípica de las células durante al menos 10 pasajes, y en donde al menos 80% de la células expresan CD9 en cinco pasajes después del pasaje 15.
MX2014010782A 2012-03-07 2013-03-06 Medios definidos para la expansion y el mantenimiento de celulas madre pluripotentes. MX354775B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261607706P 2012-03-07 2012-03-07
PCT/US2013/029360 WO2013134378A1 (en) 2012-03-07 2013-03-06 Defined media for expansion and maintenance of pluripotent stem cells

Publications (2)

Publication Number Publication Date
MX2014010782A MX2014010782A (es) 2014-10-14
MX354775B true MX354775B (es) 2018-03-20

Family

ID=49114468

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014010782A MX354775B (es) 2012-03-07 2013-03-06 Medios definidos para la expansion y el mantenimiento de celulas madre pluripotentes.

Country Status (16)

Country Link
US (3) US9434920B2 (es)
EP (1) EP2823037A4 (es)
JP (1) JP6383292B2 (es)
KR (1) KR20140131999A (es)
CN (1) CN104160018A (es)
AR (1) AR090276A1 (es)
AU (2) AU2013230020B2 (es)
CA (1) CA2866590A1 (es)
HK (1) HK1206058A1 (es)
IN (1) IN2014DN07036A (es)
MX (1) MX354775B (es)
PH (1) PH12014501898A1 (es)
RU (2) RU2018128383A (es)
SG (1) SG11201405052RA (es)
WO (1) WO2013134378A1 (es)
ZA (1) ZA201407241B (es)

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WO2014192938A1 (ja) 2013-05-30 2014-12-04 味の素株式会社 幹細胞の培養用培地
WO2015066631A2 (en) * 2013-11-01 2015-05-07 University Of Notre Dame Du Lac Cell culture medium and bioprocess optimization
EP3129466B1 (en) * 2014-04-07 2020-08-05 Memorial Sloan Kettering Cancer Center Modulating cell proliferation and pluripotency
CA2957532A1 (en) * 2014-08-07 2016-02-11 Duke University Compositions and methods for the reprogramming of cells into cardiomyocytes
US10597634B2 (en) * 2014-12-31 2020-03-24 Peking University School And Hospital Of Stomatology Device and use thereof in cell experiments in vitro
US11174461B2 (en) 2016-03-16 2021-11-16 Cynata Therapeutics Limited Colony forming medium and use thereof
KR102015815B1 (ko) * 2016-08-10 2019-08-29 가톨릭대학교 산학협력단 유도만능줄기세포의 분화를 유도하여 각막 상피 세포를 배양하는 방법 및 시스템
KR20190102011A (ko) * 2016-12-21 2019-09-02 프로메틱 파마 에스엠티 리미티드 상피-중간엽 전이를 예방 또는 최소화하는 방법 및 조성물
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