AU2013230020B2 - Defined media for expansion and maintenance of pluripotent stem cells - Google Patents

Defined media for expansion and maintenance of pluripotent stem cells Download PDF

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Publication number
AU2013230020B2
AU2013230020B2 AU2013230020A AU2013230020A AU2013230020B2 AU 2013230020 B2 AU2013230020 B2 AU 2013230020B2 AU 2013230020 A AU2013230020 A AU 2013230020A AU 2013230020 A AU2013230020 A AU 2013230020A AU 2013230020 B2 AU2013230020 B2 AU 2013230020B2
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cell culture
acid
cells
culture formulation
defined cell
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AU2013230020A1 (en
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Alireza Rezania
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Janssen Biotech Inc
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Janssen Biotech Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Reproductive Health (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2013230020A 2012-03-07 2013-03-06 Defined media for expansion and maintenance of pluripotent stem cells Ceased AU2013230020B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2018260810A AU2018260810A1 (en) 2012-03-07 2018-11-06 Defined media for expansion and maintenance of pluripotent stem cells

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261607706P 2012-03-07 2012-03-07
US61/607,706 2012-03-07
PCT/US2013/029360 WO2013134378A1 (en) 2012-03-07 2013-03-06 Defined media for expansion and maintenance of pluripotent stem cells

Related Child Applications (1)

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AU2018260810A Division AU2018260810A1 (en) 2012-03-07 2018-11-06 Defined media for expansion and maintenance of pluripotent stem cells

Publications (2)

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AU2013230020A1 AU2013230020A1 (en) 2014-09-04
AU2013230020B2 true AU2013230020B2 (en) 2018-08-09

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AU2013230020A Ceased AU2013230020B2 (en) 2012-03-07 2013-03-06 Defined media for expansion and maintenance of pluripotent stem cells
AU2018260810A Abandoned AU2018260810A1 (en) 2012-03-07 2018-11-06 Defined media for expansion and maintenance of pluripotent stem cells

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AU2018260810A Abandoned AU2018260810A1 (en) 2012-03-07 2018-11-06 Defined media for expansion and maintenance of pluripotent stem cells

Country Status (16)

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US (3) US9434920B2 (enExample)
EP (1) EP2823037A4 (enExample)
JP (1) JP6383292B2 (enExample)
KR (1) KR20140131999A (enExample)
CN (1) CN104160018A (enExample)
AR (1) AR090276A1 (enExample)
AU (2) AU2013230020B2 (enExample)
CA (1) CA2866590A1 (enExample)
HK (1) HK1206058A1 (enExample)
IN (1) IN2014DN07036A (enExample)
MX (1) MX354775B (enExample)
PH (1) PH12014501898A1 (enExample)
RU (2) RU2018128383A (enExample)
SG (1) SG11201405052RA (enExample)
WO (1) WO2013134378A1 (enExample)
ZA (1) ZA201407241B (enExample)

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CN103088065B (zh) * 2013-01-25 2014-12-10 北京银杏德济生物技术有限公司 一种能够大规模并快速高纯度地诱导间充质干细胞转决定成造血干细胞的方法
SG11201509767UA (en) 2013-05-30 2015-12-30 Ajinomoto Kk Medium for culturing stem cells
WO2015066631A2 (en) * 2013-11-01 2015-05-07 University Of Notre Dame Du Lac Cell culture medium and bioprocess optimization
EP3129466B1 (en) * 2014-04-07 2020-08-05 Memorial Sloan Kettering Cancer Center Modulating cell proliferation and pluripotency
EP3177302A4 (en) * 2014-08-07 2018-04-11 Duke University Compositions and methods for the reprogramming of cells into cardiomyocytes
US10597634B2 (en) * 2014-12-31 2020-03-24 Peking University School And Hospital Of Stomatology Device and use thereof in cell experiments in vitro
CA3017772C (en) * 2016-03-16 2023-02-21 Cynata Therapeutics Limited Colony forming medium and use thereof
KR102015815B1 (ko) * 2016-08-10 2019-08-29 가톨릭대학교 산학협력단 유도만능줄기세포의 분화를 유도하여 각막 상피 세포를 배양하는 방법 및 시스템
TW201825110A (zh) * 2016-12-21 2018-07-16 英商波麥堤克藥學Ｓｍｔ有限公司 用於預防或最小化上皮─間葉轉變之方法及組成物
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JP7364560B2 (ja) * 2018-06-15 2023-10-18 扶桑薬品工業株式会社 生殖補助医療用培地
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CA3139591C (en) 2019-05-31 2024-01-16 Timothy M. BRUHN A biocompatible membrane composite
EP3976237A1 (en) 2019-05-31 2022-04-06 W.L. Gore & Associates Inc. Cell encapsulation devices with controlled oxygen diffusion distances
EP3975926A1 (en) 2019-05-31 2022-04-06 W.L. Gore & Associates, Inc. A biocompatible membrane composite
CN112516291B (zh) * 2019-09-17 2023-07-14 通化安睿特生物制药股份有限公司 含人白蛋白的制剂及其制备方法
US11001810B1 (en) * 2019-11-11 2021-05-11 Lancell AB Serum-free human pluripotent stem cell culture medium
CN112708591B (zh) * 2020-12-24 2022-04-29 南京周子未来食品科技有限公司 一种用于肌肉干细胞体外分化的化学成分明确的培养基
CN115141798A (zh) * 2022-07-18 2022-10-04 上海食未生物科技有限公司 一种无血清培养基及其在培养肌肉干细胞中的用途和方法

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