AR004940A1 - Procedimiento para controlar la cantidad de acido sialico presente sobre una cadena lateral de oligosacaridos de una glicoproteina producida por cultivo en una celula hospedante de mamifero. - Google Patents
Procedimiento para controlar la cantidad de acido sialico presente sobre una cadena lateral de oligosacaridos de una glicoproteina producida por cultivo en una celula hospedante de mamifero.Info
- Publication number
- AR004940A1 AR004940A1 ARP960103001A AR10300196A AR004940A1 AR 004940 A1 AR004940 A1 AR 004940A1 AR P960103001 A ARP960103001 A AR P960103001A AR 10300196 A AR10300196 A AR 10300196A AR 004940 A1 AR004940 A1 AR 004940A1
- Authority
- AR
- Argentina
- Prior art keywords
- sialic acid
- cell culture
- cell
- culture
- hospedant
- Prior art date
Links
- 102000003886 Glycoproteins Human genes 0.000 title abstract 4
- 108090000288 Glycoproteins Proteins 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 4
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 title abstract 3
- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 title abstract 3
- 241000124008 Mammalia Species 0.000 title 1
- 229920001542 oligosaccharide Polymers 0.000 title 1
- 150000002482 oligosaccharides Chemical class 0.000 title 1
- 238000004113 cell culture Methods 0.000 abstract 6
- 210000004027 cell Anatomy 0.000 abstract 1
- 239000003623 enhancer Substances 0.000 abstract 1
- 210000004962 mammalian cell Anatomy 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70503—Immunoglobulin superfamily
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
- C07K14/7151—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for tumor necrosis factor [TNF], for lymphotoxin [LT]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/36—Lipids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Biomedical Technology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Rheumatology (AREA)
- Pain & Pain Management (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
La presente invencion se refiere a nuevos procedimientos para la preparacion de glicoproteinas mediante cultivos en celulas de mamiferos donde el contenido de acido sialico de la glicoproteina producida se controla en una amplia gama de va loresmanipulando el ambiente de cultivo de celulas. La invencion provee procedimientos en los cuales el contenido de acido sialico de la glicoproteina se modifica mediante cambios en los parametros de los cultivosde celulas que afectan la productivi dadespecifica de las celulas. Las realizaciones preferidas de la invencion incluyen procedimientos para elcultivo de celulas en las cuales se controla la osmolalidad del cultivo de celulas, asi como tambien la concentracion de un realzador detranscripcion durante la fase de produccion del cultivo de celulas.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/469,348 US5705364A (en) | 1995-06-06 | 1995-06-06 | Mammalian cell culture process |
Publications (1)
Publication Number | Publication Date |
---|---|
AR004940A1 true AR004940A1 (es) | 1999-04-07 |
Family
ID=23863440
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP960103001A AR004940A1 (es) | 1995-06-06 | 1996-06-06 | Procedimiento para controlar la cantidad de acido sialico presente sobre una cadena lateral de oligosacaridos de una glicoproteina producida por cultivo en una celula hospedante de mamifero. |
ARP980100640A AR011439A2 (es) | 1995-06-06 | 1998-02-12 | Procedimiento para producir quimeras de inmunoglobulina (ig) del receptor del factor de necrosis tumoral (tnfr), nuevas preparaciones igg1 del tnfr1 yuna composicion terapeutica que los contiene |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP980100640A AR011439A2 (es) | 1995-06-06 | 1998-02-12 | Procedimiento para producir quimeras de inmunoglobulina (ig) del receptor del factor de necrosis tumoral (tnfr), nuevas preparaciones igg1 del tnfr1 yuna composicion terapeutica que los contiene |
Country Status (36)
Country | Link |
---|---|
US (1) | US5705364A (es) |
EP (2) | EP1609853B2 (es) |
JP (2) | JPH11507523A (es) |
KR (1) | KR100496356B1 (es) |
CN (1) | CN1166772C (es) |
AR (2) | AR004940A1 (es) |
AT (2) | ATE425245T2 (es) |
AU (1) | AU717847B2 (es) |
BG (1) | BG63213B1 (es) |
BR (1) | BR9609150A (es) |
CA (1) | CA2220684C (es) |
CZ (1) | CZ293719B6 (es) |
DE (2) | DE69635076T3 (es) |
DK (2) | DK1609853T4 (es) |
EA (1) | EA001215B1 (es) |
ES (2) | ES2324046T5 (es) |
GE (1) | GEP20012519B (es) |
HK (2) | HK1017903A1 (es) |
HU (1) | HU226420B1 (es) |
IL (1) | IL122398A (es) |
IS (1) | IS2614B (es) |
MY (1) | MY113496A (es) |
NO (1) | NO322276B1 (es) |
NZ (1) | NZ310202A (es) |
OA (1) | OA10753A (es) |
PL (1) | PL185484B1 (es) |
PT (1) | PT1609853E (es) |
RO (1) | RO120267B1 (es) |
SA (1) | SA96170351B1 (es) |
SI (2) | SI0832189T2 (es) |
SK (1) | SK282658B6 (es) |
TR (1) | TR199701543T1 (es) |
TW (2) | TW426734B (es) |
UA (1) | UA47428C2 (es) |
WO (1) | WO1996039488A1 (es) |
ZA (1) | ZA964776B (es) |
Families Citing this family (113)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK0417563T3 (da) | 1989-09-12 | 2000-11-06 | Hoffmann La Roche | TNF-bindende proteiner |
US5705364A (en) † | 1995-06-06 | 1998-01-06 | Genentech, Inc. | Mammalian cell culture process |
US6656466B1 (en) * | 1995-06-06 | 2003-12-02 | Genetech, Inc. | Human tumor necrosis factor—immunoglobulin(TNFR1-IgG1) chimera composition |
JP4306813B2 (ja) | 1995-09-19 | 2009-08-05 | アスビオファーマ株式会社 | 動物細胞の新規培養方法 |
CN1233189A (zh) * | 1996-05-08 | 1999-10-27 | 弗·哈夫曼-拉罗切有限公司 | 用TNFR-Ig治疗哮喘 |
US6673575B1 (en) * | 1997-12-03 | 2004-01-06 | Roche Diagnostics Gmbh | Method for preparing polypeptides with appropriate glycosilation |
US6140445A (en) * | 1998-04-17 | 2000-10-31 | Crompton Corporation | Silane functional oligomer |
EP1092037B1 (en) * | 1998-05-29 | 2005-12-28 | Genentech, Inc. | Cell culture process for producing glycoproteins |
US6528286B1 (en) * | 1998-05-29 | 2003-03-04 | Genentech, Inc. | Mammalian cell culture process for producing glycoproteins |
TR200504220T2 (tr) | 1998-12-17 | 2007-04-24 | Biogen Idec Ma Inc. | Aktif limfotoksin-beta reseptör imunoglobülin şimeAktif limfotoksin-beta reseptör imunoglobülin şimerik proteinlerinin yüksek düzey ifadesi ve saflaştrik proteinlerinin yüksek düzey ifadesi ve saflaştırılması için bir yöntem.ırılması için bir yöntem. |
GB9828624D0 (en) * | 1998-12-23 | 1999-02-17 | Glaxo Group Ltd | Production of proteins |
KR100475417B1 (ko) * | 1998-12-30 | 2005-07-18 | 씨제이 주식회사 | 시알산 함량이 높은 재조합 당단백질의 제조방법 |
US7294481B1 (en) * | 1999-01-05 | 2007-11-13 | Immunex Corporation | Method for producing recombinant proteins |
ATE348173T1 (de) | 1999-04-26 | 2007-01-15 | Genentech Inc | Zellenzuchtverfahren für glycoproteine |
US6261805B1 (en) * | 1999-07-15 | 2001-07-17 | Boyce Thompson Institute For Plant Research, Inc. | Sialyiation of N-linked glycoproteins in the baculovirus expression vector system |
KR100394474B1 (ko) * | 1999-12-18 | 2003-08-09 | 동아제약 주식회사 | 동물세포에서 재조합 당단백질의 연속식 대량생산 방법 |
ATE313554T1 (de) | 2000-05-16 | 2006-01-15 | Lipoxen Technologies Ltd | Derivatisierung von proteinen in wässrigem lösungsmittel |
WO2002002793A1 (fr) * | 2000-07-05 | 2002-01-10 | Japan As Represented By Secretary Of Osaka University | Processus de production de glycoproteine |
US20030040095A1 (en) * | 2001-03-16 | 2003-02-27 | Achille Arini | Method for the production of pharmaceutically active recombinant proteins |
US20030087372A1 (en) * | 2001-06-13 | 2003-05-08 | Genentech, Inc. | Methods of culturing animal cells and polypeptide production in animal cells |
AU2003220529B2 (en) | 2002-03-27 | 2006-09-07 | Immunex Corporation | Methods for increasing polypeptide production |
US20030190710A1 (en) * | 2002-03-28 | 2003-10-09 | Devries Ruth L. | Control of glycoforms in IgG |
US6924124B1 (en) * | 2002-08-23 | 2005-08-02 | Immunex Corporation | Feeding strategies for cell culture |
US7067279B1 (en) | 2002-08-23 | 2006-06-27 | Immunex Corporation | Cell culture performance with betaine |
US6974681B1 (en) | 2002-08-23 | 2005-12-13 | Immunex Corporation | Cell culture performance with vanadate |
JP4754219B2 (ja) * | 2002-12-02 | 2011-08-24 | アムジエン・フレモント・インコーポレイテツド | 腫瘍壊死因子を対象とする抗体、およびそれらの使用 |
CN101857851A (zh) * | 2002-12-23 | 2010-10-13 | 布里斯托尔-迈尔斯斯奎布公司 | 用于蛋白质生产的哺乳动物细胞培养方法 |
BR0316882A (pt) * | 2002-12-23 | 2005-10-25 | Bristol Myers Squibb Co | Melhora na qualidade de produtos em processos de cultura de células de mamìferos para a produção de proteìnas |
US20040265964A1 (en) * | 2003-04-25 | 2004-12-30 | Martin Allen | Inducers of recombinant protein expression |
KR101229989B1 (ko) | 2003-10-24 | 2013-02-06 | 셀렉시스 에스. 에이. | Mar 서열의 복합 트랜스펙션 방법에 의한 포유동물 세포에서의 고효율 유전자 전달 및 발현 |
EP1697414A2 (en) * | 2003-12-23 | 2006-09-06 | Applied Research Systems ARS Holding N.V. | Process for the production of tumor necrosis factor-binding proteins |
WO2005064002A1 (en) * | 2003-12-31 | 2005-07-14 | Samyang Genex Corporation | Method for mass production of secondary metabolites in plant cell culture by treatment of an alkanoic acid or salt thereof |
US8609370B2 (en) * | 2004-02-13 | 2013-12-17 | Glycotope Gmbh | Highly active glycoproteins-process conditions and an efficient method for their production |
US7335491B2 (en) * | 2004-08-27 | 2008-02-26 | Wyeth Research Ireland Limited | Production of anti-abeta |
US7300773B2 (en) * | 2004-08-27 | 2007-11-27 | Wyeth Research Ireland Limited | Production of TNFR-Ig |
TWI384069B (zh) * | 2004-08-27 | 2013-02-01 | Pfizer Ireland Pharmaceuticals | 多胜肽之製法 |
AU2006247039B2 (en) * | 2005-05-19 | 2011-03-03 | Amgen Inc. | Compositions and methods for increasing the stability of antibodies |
AU2006254217A1 (en) * | 2005-06-03 | 2006-12-07 | Biovitrum Ab (Publ) | Process for cultivating animal cells comprising the feeding of plant-derived peptones |
KR100670105B1 (ko) | 2005-06-29 | 2007-01-17 | 주식회사 셀트리온 | 콩가수분해물의 저분자량 분획을 이용하여 시알산 함량이증가된 에리스로포이에틴을 생산하는 방법 및 그에 의하여생산된 시알산 함량이 증가된 에리스로포이에틴 |
US8470318B2 (en) | 2005-11-07 | 2013-06-25 | The Rockefeller University | Polypeptides with enhanced anti-inflammatory and decreased cytotoxic properties and relating methods |
WO2008057634A2 (en) * | 2006-10-26 | 2008-05-15 | The Rockefeller University | Polypeptides with enhanced anti-inflammatory and decreased cytotoxic properties and relating methods |
AR058568A1 (es) | 2005-12-20 | 2008-02-13 | Bristol Myers Squibb Co | Metodos para producir una composicion con moleculas ctla4-ig a partir de un medio de cultivo |
PL1969007T4 (pl) | 2005-12-20 | 2014-04-30 | Bristol Myers Squibb Co | Kompozycje i sposoby wytwarzania kompozycji |
US20070190057A1 (en) | 2006-01-23 | 2007-08-16 | Jian Wu | Methods for modulating mannose content of recombinant proteins |
EP2264060B1 (en) * | 2006-01-26 | 2014-04-23 | Recopharma AB | Compositions and methods for inhibiting viral adhesion |
NZ572379A (en) * | 2006-04-05 | 2012-06-29 | Univ Rockefeller | Polypeptides with enhanced anti-inflammatory and decreased cytotoxic properties and relating methods |
CA2657248C (en) | 2006-07-13 | 2018-10-30 | Wyeth | Production of glycoproteins |
US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
WO2008033517A2 (en) | 2006-09-13 | 2008-03-20 | Abbott Laboratories | Cell culture improvements |
US20080145893A1 (en) * | 2006-09-17 | 2008-06-19 | Excellegene Sa | Method for producing a recombinant protein at high specific productivity, high batch yield and high volumetric yield by means of transient transfection |
WO2008069244A1 (ja) * | 2006-12-05 | 2008-06-12 | Kyowa Hakko Kogyo Co., Ltd. | 糖蛋白質組成物の製造方法 |
JP5175336B2 (ja) * | 2007-04-16 | 2013-04-03 | モメンタ ファーマシューティカルズ インコーポレイテッド | 細胞表面グリコシル化に関連する方法 |
TW200902708A (en) | 2007-04-23 | 2009-01-16 | Wyeth Corp | Methods of protein production using anti-senescence compounds |
US20090023186A1 (en) * | 2007-07-22 | 2009-01-22 | Excellgene Sa | Use of valproic acid for enhancing production of recombinant proteins in mammalian cells |
ES2657055T3 (es) * | 2007-08-09 | 2018-03-01 | Wyeth Llc | Uso de perfusión para mejorar la producción de un cultivo de células alimentado por lotes en biorreactores |
TWI395593B (zh) * | 2008-03-06 | 2013-05-11 | Halozyme Inc | 可活化的基質降解酵素之活體內暫時性控制 |
US8187855B2 (en) | 2008-03-06 | 2012-05-29 | Halozyme, Inc. | Large-scale production of soluble hyaluronidase |
EP2283043B1 (en) * | 2008-04-07 | 2014-08-13 | Bayer HealthCare, LLC | Methods of recombinant production of glycoproteins |
US7846744B2 (en) | 2008-04-22 | 2010-12-07 | Ravetch Jeffrey V | Methods of identifying anti-inflammatory compounds |
WO2010031074A2 (en) | 2008-09-15 | 2010-03-18 | Genentech, Inc. | Compositions and methods for regulating cell osmolarity |
CN104974251A (zh) | 2008-10-20 | 2015-10-14 | Abbvie公司 | 在抗体纯化过程中的病毒灭活 |
EP2921501A1 (en) * | 2008-10-20 | 2015-09-23 | Abbvie Inc. | Isolation and purification of antibodies using Protein A affinity chromatography |
CA2746181C (en) * | 2008-12-09 | 2016-03-15 | Halozyme, Inc. | Extended soluble ph20 polypeptides and uses thereof |
WO2010102262A1 (en) * | 2009-03-06 | 2010-09-10 | Halozyme, Inc. | Temperature sensitive mutants of matrix metalloprotease 1 und uses thereof |
US9540426B2 (en) | 2009-10-06 | 2017-01-10 | Bristol-Myers Squibb Company | Mammalian cell culture processes for protein production |
AU2010311567B2 (en) | 2009-10-26 | 2015-03-26 | Chugai Seiyaku Kabushiki Kaisha | Method for the production of a glycosylated immunoglobulin |
US8765415B2 (en) | 2009-11-17 | 2014-07-01 | Medarex, L.L.C. | Methods for enhanced protein production |
US8722615B2 (en) * | 2009-12-02 | 2014-05-13 | Acceleron Pharma, Inc. | Compositions and methods for increasing serum half-life |
PL3281636T3 (pl) * | 2010-02-24 | 2021-03-08 | Chiesi Farmaceutici S.P.A. | Sposób wytwarzania i oczyszczania rekombinowanej lizosomalnej alfa-mannozydazy |
JPWO2012017925A1 (ja) | 2010-08-02 | 2013-10-03 | 協和発酵キリン株式会社 | 物質の製造方法 |
EP2686423A4 (en) | 2011-03-14 | 2015-01-28 | Nat Res Council Canada | METHOD FOR PRODUCING VIRUSES IN CELLS |
EP2702077A2 (en) | 2011-04-27 | 2014-03-05 | AbbVie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
DK2702164T3 (en) | 2011-04-29 | 2016-02-01 | Biocon Res Ltd | METHOD FOR REDUCING heterogeneity OF ANTIBODIES AND METHOD OF PRODUCING THESE ANTIBODIES |
WO2012170938A1 (en) | 2011-06-08 | 2012-12-13 | Acceleron Pharma Inc. | Compositions and methods for increasing serum half-life |
WO2013158273A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Methods to modulate c-terminal lysine variant distribution |
US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
WO2013158279A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Protein purification methods to reduce acidic species |
WO2013176754A1 (en) | 2012-05-24 | 2013-11-28 | Abbvie Inc. | Novel purification of antibodies using hydrophobic interaction chromatography |
CN102839157A (zh) * | 2012-07-12 | 2012-12-26 | 扬州大学 | 稳定共表达hST3GalIV和β1-4GalT1的MDCK细胞系及构建方法和应用 |
US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
WO2014035475A1 (en) | 2012-09-02 | 2014-03-06 | Abbvie Inc. | Methods to control protein heterogeneity |
NO2760138T3 (es) | 2012-10-01 | 2018-08-04 | ||
US20140106405A1 (en) * | 2012-10-15 | 2014-04-17 | Bristol-Myers Squibb Company | Mammalian cell culture processes for protein production |
WO2014062535A1 (en) | 2012-10-15 | 2014-04-24 | Bristol-Myers Squibb Company | Mammalian cell culture processes for protein production |
AU2013381687A1 (en) | 2013-03-12 | 2015-09-24 | Abbvie Inc. | Human antibodies that bind human TNF-alpha and methods of preparing the same |
US9499614B2 (en) | 2013-03-14 | 2016-11-22 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides |
US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
WO2014159579A1 (en) | 2013-03-14 | 2014-10-02 | Abbvie Inc. | MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE |
US9217168B2 (en) | 2013-03-14 | 2015-12-22 | Momenta Pharmaceuticals, Inc. | Methods of cell culture |
AR095196A1 (es) | 2013-03-15 | 2015-09-30 | Regeneron Pharma | Medio de cultivo celular libre de suero |
MX363403B (es) | 2013-07-04 | 2019-03-22 | Hoffmann La Roche | Inmumoensayo con interferencia suprimida para la deteccion de anticuerpos anti-farmacos en muestras de suero. |
MX2016000123A (es) | 2013-07-06 | 2016-07-14 | Cadila Healthcare Ltd | Procedimiento mejorado para la produccion de anticuerpos monoclonales. |
US9598667B2 (en) | 2013-10-04 | 2017-03-21 | Abbvie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
US8946395B1 (en) | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
US20150139988A1 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
CA2937611C (en) * | 2014-02-27 | 2023-01-03 | F. Hoffmann-La Roche Ag | Modulation of cell growth and glycosylation in recombinant glycoprotein production |
JP6652334B2 (ja) | 2014-05-31 | 2020-02-19 | Jcrファーマ株式会社 | ウリジンとn−アセチル−d−マンノサミンとを含有する培地 |
MA40021A (fr) * | 2014-06-03 | 2015-12-10 | Lupin Ltd | Processus de culture cellulaire destiné à produire une protéine |
US20160130324A1 (en) | 2014-10-31 | 2016-05-12 | Shire Human Genetic Therapies, Inc. | C1 Inhibitor Fusion Proteins and Uses Thereof |
KR102007930B1 (ko) | 2014-12-31 | 2019-08-06 | 주식회사 엘지화학 | 재조합 당단백질의 글리코실화 조절 방법 |
TW202330904A (zh) | 2015-08-04 | 2023-08-01 | 美商再生元醫藥公司 | 補充牛磺酸之細胞培養基及用法 |
US20180334493A1 (en) | 2015-11-19 | 2018-11-22 | Shire Human Genetic Therapies, Inc. | Recombinant human c1 esterase inhibitor and uses thereof |
EP3397076A1 (en) * | 2015-12-29 | 2018-11-07 | N.V. Nutricia | Fermented formula with non-digestible oligosaccharides |
CN111406112A (zh) * | 2017-11-30 | 2020-07-10 | 豪夫迈·罗氏有限公司 | 用于培养哺乳动物细胞的工艺 |
TW202039849A (zh) | 2018-11-13 | 2020-11-01 | 美商健生生物科技公司 | 在產生抗cd38抗體之期間微量金屬的控制 |
BR112021025438A2 (pt) | 2019-12-06 | 2022-06-21 | Regeneron Pharma | Composições de proteínas anti-vegf e métodos para produzir as mesmas |
BR112023003273A2 (pt) | 2020-08-31 | 2023-05-02 | Regeneron Pharma | Estratégias de alimentação de asparagina para melhorar desempenho da cultura celular e mitigar variantes da sequência de asparagina |
KR20230134117A (ko) | 2021-01-20 | 2023-09-20 | 리제너론 파마슈티칼스 인코포레이티드 | 세포 배양물에서 단백질 역가를 개선시키는 방법 |
AU2022359898A1 (en) | 2021-10-07 | 2024-03-21 | Regeneron Pharmaceuticals, Inc. | Ph meter calibration and correction |
TW202323817A (zh) | 2021-10-07 | 2023-06-16 | 美商再生元醫藥公司 | Ph建模及控制之系統及方法 |
US20230331776A1 (en) | 2022-03-02 | 2023-10-19 | Regeneron Pharmaceuticals, Inc. | Manufacturing process for high titer antibody |
Family Cites Families (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3926723A (en) | 1974-08-08 | 1975-12-16 | Massachusetts Inst Technology | Method of controllably releasing glucose to a cell culture medium |
JPS5938488B2 (ja) | 1979-05-07 | 1984-09-17 | 株式会社東芝 | 空気調和機 |
EP0097353B1 (en) * | 1982-06-21 | 1990-08-16 | The Wellcome Foundation Limited | Improved process for interferon production |
US4560655A (en) | 1982-12-16 | 1985-12-24 | Immunex Corporation | Serum-free cell culture medium and process for making same |
US4657866A (en) | 1982-12-21 | 1987-04-14 | Sudhir Kumar | Serum-free, synthetic, completely chemically defined tissue culture media |
US4767704A (en) | 1983-10-07 | 1988-08-30 | Columbia University In The City Of New York | Protein-free culture medium |
US4724206A (en) * | 1984-02-13 | 1988-02-09 | Damon Biotech, Inc. | Protein production using hypertonic media |
GB8516415D0 (en) | 1985-06-28 | 1985-07-31 | Celltech Ltd | Culture of animal cells |
JPH0639919B2 (ja) | 1985-08-29 | 1994-05-25 | トヨタ自動車株式会社 | 過給機付き内燃機関の異常判別装置 |
GB8606386D0 (en) * | 1986-03-14 | 1986-04-23 | Celltech Ltd | Production of protein |
US4927762A (en) | 1986-04-01 | 1990-05-22 | Cell Enterprises, Inc. | Cell culture medium with antioxidant |
WO1988001643A1 (en) | 1986-08-29 | 1988-03-10 | Endotronics, Inc. | Method of culturing cells |
JPH01257492A (ja) * | 1987-03-05 | 1989-10-13 | Green Cross Corp:The | 異種蛋白質の生産増強方法 |
IL87737A (en) * | 1987-09-11 | 1993-08-18 | Genentech Inc | Method for culturing polypeptide factor dependent vertebrate recombinant cells |
JPH066054B2 (ja) | 1987-10-15 | 1994-01-26 | 帝人株式会社 | 動物細胞の培養方法 |
CA1312030C (en) * | 1987-11-18 | 1992-12-29 | Brian Maiorella | Method to increase antibody titer |
US5151359A (en) * | 1988-05-19 | 1992-09-29 | Mitsui Toatsu Chemicals Incorporated | Method for producing of human tissue type plasminogen activator |
EP0435911B1 (en) | 1988-09-23 | 1996-03-13 | Cetus Oncology Corporation | Cell culture medium for enhanced cell growth, culture longevity and product expression |
KR0132666B1 (en) * | 1989-03-14 | 1998-04-14 | Hitachi Kk | Method for controlling cultivation conditions for animal cells |
JPH0396383A (ja) | 1989-09-08 | 1991-04-22 | Riso Kagaku Corp | 画像形成装置 |
DK0417563T3 (da) | 1989-09-12 | 2000-11-06 | Hoffmann La Roche | TNF-bindende proteiner |
US5096816A (en) * | 1990-06-05 | 1992-03-17 | Cetus Corporation | In vitro management of ammonia's effect on glycosylation of cell products through pH control |
US5122469A (en) * | 1990-10-03 | 1992-06-16 | Genentech, Inc. | Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins |
GB9022545D0 (en) * | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
GB2251249B (en) * | 1990-12-28 | 1995-06-21 | Mogam Biotech Res Inst | High-density medium for animal cell culture |
WO1993010260A1 (en) * | 1991-11-21 | 1993-05-27 | The Board Of Trustees Of The Leland Stanford Junior University | Controlling degradation of glycoprotein oligosaccharides by extracellular glycosisases |
US5447851B1 (en) * | 1992-04-02 | 1999-07-06 | Univ Texas System Board Of | Dna encoding a chimeric polypeptide comprising the extracellular domain of tnf receptor fused to igg vectors and host cells |
KR100232688B1 (ko) * | 1992-09-15 | 1999-12-01 | 스코트 쥐. 홀퀴스트 | 종양 괴사 인자 길항제를 함유하는 tnf-의존성 염증 치료용 제약 조성물 |
JP3523293B2 (ja) † | 1993-07-19 | 2004-04-26 | 住友製薬株式会社 | インターフェロンの産生増強法 |
US5705364A (en) † | 1995-06-06 | 1998-01-06 | Genentech, Inc. | Mammalian cell culture process |
JP5515221B2 (ja) † | 2008-01-28 | 2014-06-11 | 日油株式会社 | ポリオキシエチレンソルビタン脂肪酸エステルの製造方法 |
-
1995
- 1995-06-06 US US08/469,348 patent/US5705364A/en not_active Expired - Lifetime
-
1996
- 1996-06-05 TW TW085106712A patent/TW426734B/zh not_active IP Right Cessation
- 1996-06-05 TW TW089111534A patent/TW516962B/zh not_active IP Right Cessation
- 1996-06-06 AR ARP960103001A patent/AR004940A1/es active IP Right Grant
- 1996-06-06 CA CA002220684A patent/CA2220684C/en not_active Expired - Lifetime
- 1996-06-06 IL IL12239896A patent/IL122398A/en not_active IP Right Cessation
- 1996-06-06 CN CNB961944498A patent/CN1166772C/zh not_active Expired - Lifetime
- 1996-06-06 AT AT05017434T patent/ATE425245T2/de active
- 1996-06-06 DE DE69635076T patent/DE69635076T3/de not_active Expired - Lifetime
- 1996-06-06 SK SK1670-97A patent/SK282658B6/sk not_active IP Right Cessation
- 1996-06-06 PL PL96323737A patent/PL185484B1/pl unknown
- 1996-06-06 SI SI9630715T patent/SI0832189T2/sl unknown
- 1996-06-06 DK DK05017434.1T patent/DK1609853T4/da active
- 1996-06-06 UA UA97125856A patent/UA47428C2/uk unknown
- 1996-06-06 CZ CZ19973909A patent/CZ293719B6/cs not_active IP Right Cessation
- 1996-06-06 EP EP05017434.1A patent/EP1609853B2/en not_active Expired - Lifetime
- 1996-06-06 EP EP96918251A patent/EP0832189B2/en not_active Expired - Lifetime
- 1996-06-06 HU HU9900920A patent/HU226420B1/hu unknown
- 1996-06-06 MY MYPI96002265A patent/MY113496A/en unknown
- 1996-06-06 ES ES05017434T patent/ES2324046T5/es not_active Expired - Lifetime
- 1996-06-06 GE GEAP19964011A patent/GEP20012519B/en unknown
- 1996-06-06 TR TR97/01543T patent/TR199701543T1/xx unknown
- 1996-06-06 BR BR9609150A patent/BR9609150A/pt not_active Application Discontinuation
- 1996-06-06 PT PT05017434T patent/PT1609853E/pt unknown
- 1996-06-06 KR KR1019970708954A patent/KR100496356B1/ko not_active IP Right Cessation
- 1996-06-06 NZ NZ310202A patent/NZ310202A/en not_active IP Right Cessation
- 1996-06-06 ZA ZA9604776A patent/ZA964776B/xx unknown
- 1996-06-06 SI SI9630767T patent/SI1609853T2/sl unknown
- 1996-06-06 DE DE69637867T patent/DE69637867D1/de not_active Expired - Lifetime
- 1996-06-06 AT AT96918251T patent/ATE302266T1/de active
- 1996-06-06 RO RO97-02262A patent/RO120267B1/ro unknown
- 1996-06-06 AU AU60952/96A patent/AU717847B2/en not_active Expired
- 1996-06-06 ES ES96918251T patent/ES2248812T5/es not_active Expired - Lifetime
- 1996-06-06 EA EA199700364A patent/EA001215B1/ru not_active IP Right Cessation
- 1996-06-06 DK DK96918251T patent/DK0832189T4/da active
- 1996-06-06 JP JP9501638A patent/JPH11507523A/ja not_active Withdrawn
- 1996-06-06 WO PCT/US1996/009284 patent/WO1996039488A1/en active IP Right Grant
- 1996-10-07 SA SA96170351A patent/SA96170351B1/ar unknown
-
1997
- 1997-12-03 IS IS4626A patent/IS2614B/is unknown
- 1997-12-03 BG BG102101A patent/BG63213B1/bg unknown
- 1997-12-05 NO NO19975674A patent/NO322276B1/no not_active IP Right Cessation
- 1997-12-05 OA OA70153A patent/OA10753A/en unknown
-
1998
- 1998-02-12 AR ARP980100640A patent/AR011439A2/es unknown
- 1998-12-23 HK HK98114966A patent/HK1017903A1/xx not_active IP Right Cessation
-
2006
- 2006-06-28 HK HK06107347.9A patent/HK1087151A1/xx not_active IP Right Cessation
-
2007
- 2007-02-28 JP JP2007050848A patent/JP4348376B2/ja not_active Expired - Lifetime
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AR004940A1 (es) | Procedimiento para controlar la cantidad de acido sialico presente sobre una cadena lateral de oligosacaridos de una glicoproteina producida por cultivo en una celula hospedante de mamifero. | |
AR078544A1 (es) | Procedimiento de cultivo de celulas de mamiferos para la produccion de proteinas | |
Scoma et al. | Sustained H2 production in a Chlamydomonas reinhardtii D1 protein mutant | |
Renvoisé et al. | Emerging themes of ER organization in the development and maintenance of axons | |
TW200512297A (en) | Mammalian cell culture processes for protein production | |
MX172674B (es) | Procedimiento mejorado para la produccion de la hormona de crecimiento humano, mediante transformantes bacterianos | |
DE60026142D1 (de) | Verfahren zur herstellung von oligosaccharide | |
BR0316882A (pt) | Melhora na qualidade de produtos em processos de cultura de células de mamìferos para a produção de proteìnas | |
CY1107771T1 (el) | Μεθοδος για την παραγωγη χονδρινων εμφυτευματων απο in vitro καλλιεργημενα χονδροκυτταρα | |
FI93310B (fi) | Menetelmä rokotteen valmistamiseksi piluksia kantavien nisäkkään kudoksiin tarttuvien bakteerien aiheuttamia tauteja vastaan | |
AR061976A1 (es) | Produccion de glicoproteinas | |
ES2133163T3 (es) | Esteres (o tioesteres) del acido n-acilsulfamico y esteres (o tioesteres) del acido n-sulfonilcarbamico como agentes hipercolesterolemicos. | |
BRPI0606117A2 (pt) | método de geração de ácido l-ascórbico, levedura recombinante e método para aumentar a produção, a produtividade, ou o rendimento de um produto produzido por um microorganismo durante a fermentação | |
JP2011518790A5 (es) | ||
ES2556454T3 (es) | Proceso para producción de proteínas | |
AU4314299A (en) | Cell culture process for producing glycoproteins | |
WO2006097701A3 (en) | Skin equivalent culture | |
FR2389673A1 (es) | ||
EP0245481A4 (en) | Yeast strains. | |
Voccoli et al. | Mitochondrial remodeling in differentiating neuroblasts | |
KR20180018171A (ko) | 강염기를 이용한 이산화탄소 고정을 통한 미세조류 배양방법 | |
PT1146117E (pt) | Um método para a produção de preparações de células endócrinas pancreáticas maduras e imaturas, a preparação de células e seu uso para tratamento de diabetes mellitus | |
Aziz et al. | The origin of the nascent blastocoele in preimplantation mouse embryos ultrastructural cytochemistry and effect of chloroquine | |
ATE299178T1 (de) | Verfahren zur herstellung von säugetier-zellinien | |
BR112023003273A2 (pt) | Estratégias de alimentação de asparagina para melhorar desempenho da cultura celular e mitigar variantes da sequência de asparagina |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG | Grant, registration |