ZA201708498B - Crispr enzyme mutations reducing off¿target effects - Google Patents

Crispr enzyme mutations reducing off¿target effects

Info

Publication number
ZA201708498B
ZA201708498B ZA2017/08498A ZA201708498A ZA201708498B ZA 201708498 B ZA201708498 B ZA 201708498B ZA 2017/08498 A ZA2017/08498 A ZA 2017/08498A ZA 201708498 A ZA201708498 A ZA 201708498A ZA 201708498 B ZA201708498 B ZA 201708498B
Authority
ZA
South Africa
Prior art keywords
crispr enzyme
target effects
mutations reducing
enzyme mutations
reducing
Prior art date
Application number
ZA2017/08498A
Other languages
English (en)
Inventor
Feng Zhang
Linyi Gao
Bernd Zetsche
Ian Slaymaker
Original Assignee
Broad Inst Inc
Massachusetts Inst Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Broad Inst Inc, Massachusetts Inst Technology filed Critical Broad Inst Inc
Publication of ZA201708498B publication Critical patent/ZA201708498B/en

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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • G—PHYSICS
    • G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
    • G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
    • G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
    • G09B23/28—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for medicine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Health & Medical Sciences (AREA)
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  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Business, Economics & Management (AREA)
  • Computational Mathematics (AREA)
  • Educational Technology (AREA)
  • Educational Administration (AREA)
  • Pure & Applied Mathematics (AREA)
  • Mathematical Physics (AREA)
  • Mathematical Optimization (AREA)
  • Mathematical Analysis (AREA)
  • Cell Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Algebra (AREA)
  • Medical Informatics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
ZA2017/08498A 2015-06-18 2017-12-14 Crispr enzyme mutations reducing off¿target effects ZA201708498B (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201562181453P 2015-06-18 2015-06-18
US201562207312P 2015-08-19 2015-08-19
US201562237360P 2015-10-05 2015-10-05
US201562255256P 2015-11-13 2015-11-13
US201562269876P 2015-12-18 2015-12-18
PCT/US2016/038034 WO2016205613A1 (en) 2015-06-18 2016-06-17 Crispr enzyme mutations reducing off-target effects

Publications (1)

Publication Number Publication Date
ZA201708498B true ZA201708498B (en) 2018-11-28

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
ZA2017/08498A ZA201708498B (en) 2015-06-18 2017-12-14 Crispr enzyme mutations reducing off¿target effects

Country Status (14)

Country Link
US (4) US10876100B2 (https=)
EP (2) EP3929287A3 (https=)
JP (3) JP7107683B2 (https=)
KR (2) KR102840885B1 (https=)
CN (2) CN108290933A (https=)
AU (2) AU2016280893B2 (https=)
CA (1) CA2989830A1 (https=)
IL (3) IL293323B2 (https=)
MX (2) MX392008B (https=)
RU (1) RU2752834C2 (https=)
SG (1) SG10201912329YA (https=)
TW (2) TWI906646B (https=)
WO (1) WO2016205613A1 (https=)
ZA (1) ZA201708498B (https=)

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