KR102840885B1 - 표적외 효과를 감소시키는 crispr 효소 돌연변이 - Google Patents

표적외 효과를 감소시키는 crispr 효소 돌연변이

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KR102840885B1
KR102840885B1 KR1020237029777A KR20237029777A KR102840885B1 KR 102840885 B1 KR102840885 B1 KR 102840885B1 KR 1020237029777 A KR1020237029777 A KR 1020237029777A KR 20237029777 A KR20237029777 A KR 20237029777A KR 102840885 B1 KR102840885 B1 KR 102840885B1
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sequence
crispr
target
enzyme
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KR20230132877A (ko
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펭 장
린이 가오
베른트 체체
이안 슬레이메이커
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더 브로드 인스티튜트, 인코퍼레이티드
메사추세츠 인스티튜트 오브 테크놀로지
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • G—PHYSICS
    • G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
    • G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
    • G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
    • G09B23/28—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for medicine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Medical Informatics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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KR1020237029777A 2015-06-18 2016-06-17 표적외 효과를 감소시키는 crispr 효소 돌연변이 Active KR102840885B1 (ko)

Applications Claiming Priority (12)

Application Number Priority Date Filing Date Title
US201562181453P 2015-06-18 2015-06-18
US62/181,453 2015-06-18
US201562207312P 2015-08-19 2015-08-19
US62/207,312 2015-08-19
US201562237360P 2015-10-05 2015-10-05
US62/237,360 2015-10-05
US201562255256P 2015-11-13 2015-11-13
US62/255,256 2015-11-13
US201562269876P 2015-12-18 2015-12-18
US62/269,876 2015-12-18
KR1020187001739A KR102575342B1 (ko) 2015-06-18 2016-06-17 표적외 효과를 감소시키는 crispr 효소 돌연변이
PCT/US2016/038034 WO2016205613A1 (en) 2015-06-18 2016-06-17 Crispr enzyme mutations reducing off-target effects

Related Parent Applications (1)

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KR1020187001739A Division KR102575342B1 (ko) 2015-06-18 2016-06-17 표적외 효과를 감소시키는 crispr 효소 돌연변이

Publications (2)

Publication Number Publication Date
KR20230132877A KR20230132877A (ko) 2023-09-18
KR102840885B1 true KR102840885B1 (ko) 2025-07-30

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KR1020237029777A Active KR102840885B1 (ko) 2015-06-18 2016-06-17 표적외 효과를 감소시키는 crispr 효소 돌연변이
KR1020187001739A Active KR102575342B1 (ko) 2015-06-18 2016-06-17 표적외 효과를 감소시키는 crispr 효소 돌연변이

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Country Link
US (4) US10876100B2 (https=)
EP (2) EP3929287A3 (https=)
JP (3) JP7107683B2 (https=)
KR (2) KR102840885B1 (https=)
CN (2) CN108290933A (https=)
AU (2) AU2016280893B2 (https=)
CA (1) CA2989830A1 (https=)
IL (3) IL293323B2 (https=)
MX (2) MX392008B (https=)
RU (1) RU2752834C2 (https=)
SG (1) SG10201912329YA (https=)
TW (2) TWI906646B (https=)
WO (1) WO2016205613A1 (https=)
ZA (1) ZA201708498B (https=)

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