DK2601611T3 - Fremgangsmåder til forudsigelse af stabilitet og smeltetemperaturer for nukleinsyreduplekser - Google Patents
Fremgangsmåder til forudsigelse af stabilitet og smeltetemperaturer for nukleinsyreduplekser Download PDFInfo
- Publication number
- DK2601611T3 DK2601611T3 DK11749613.3T DK11749613T DK2601611T3 DK 2601611 T3 DK2601611 T3 DK 2601611T3 DK 11749613 T DK11749613 T DK 11749613T DK 2601611 T3 DK2601611 T3 DK 2601611T3
- Authority
- DK
- Denmark
- Prior art keywords
- procedures
- nucleic acid
- melting temperatures
- acid duplexes
- predicting stability
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
- G01K11/00—Measuring temperature based upon physical or chemical changes not covered by groups G01K3/00, G01K5/00, G01K7/00 or G01K9/00
- G01K11/06—Measuring temperature based upon physical or chemical changes not covered by groups G01K3/00, G01K5/00, G01K7/00 or G01K9/00 using melting, freezing, or softening
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
- G16B25/20—Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US37004010P | 2010-08-02 | 2010-08-02 | |
PCT/US2011/046010 WO2012018697A1 (en) | 2010-08-02 | 2011-07-29 | Methods for predicting stability and melting temperatures of nucleic acid duplexes |
Publications (1)
Publication Number | Publication Date |
---|---|
DK2601611T3 true DK2601611T3 (da) | 2021-02-01 |
Family
ID=44533131
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK11749613.3T DK2601611T3 (da) | 2010-08-02 | 2011-07-29 | Fremgangsmåder til forudsigelse af stabilitet og smeltetemperaturer for nukleinsyreduplekser |
Country Status (4)
Country | Link |
---|---|
US (1) | US9081737B2 (da) |
EP (1) | EP2601611B1 (da) |
DK (1) | DK2601611T3 (da) |
WO (1) | WO2012018697A1 (da) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
PL2800811T3 (pl) | 2012-05-25 | 2017-11-30 | Emmanuelle Charpentier | Sposoby i kompozycje do kierowanej RNA modyfikacji docelowego DNA i do modulacji transkrypcji kierowanej RNA |
US10266889B2 (en) | 2012-07-03 | 2019-04-23 | Roche Finance Ltd | Tm-enhanced blocking oligonucleotides and baits for improved target enrichment and reduced off-target selection |
PT3363902T (pt) | 2012-12-06 | 2019-12-19 | Sigma Aldrich Co Llc | Modificação e regulação de genoma baseadas em crispr |
ES2701749T3 (es) * | 2012-12-12 | 2019-02-25 | Broad Inst Inc | Métodos, modelos, sistemas y aparatos para identificar secuencias diana para enzimas Cas o sistemas CRISPR-Cas para secuencias diana y transmitir resultados de los mismos |
KR20150105635A (ko) | 2012-12-12 | 2015-09-17 | 더 브로드 인스티튜트, 인코퍼레이티드 | 서열 조작을 위한 crispr-cas 성분 시스템, 방법 및 조성물 |
SG10201912328UA (en) | 2012-12-12 | 2020-02-27 | Broad Inst Inc | Delivery, Engineering and Optimization of Systems, Methods and Compositions for Sequence Manipulation and Therapeutic Applications |
US20140315985A1 (en) | 2013-03-14 | 2014-10-23 | Caribou Biosciences, Inc. | Compositions and methods of nucleic acid-targeting nucleic acids |
EP3825406A1 (en) | 2013-06-17 | 2021-05-26 | The Broad Institute Inc. | Delivery and use of the crispr-cas systems, vectors and compositions for hepatic targeting and therapy |
WO2014204727A1 (en) | 2013-06-17 | 2014-12-24 | The Broad Institute Inc. | Functional genomics using crispr-cas systems, compositions methods, screens and applications thereof |
RU2716421C2 (ru) | 2013-06-17 | 2020-03-11 | Те Брод Инститьют Инк. | Доставка, применение и применения в терапии систем crispr-cas и композиций для целенаправленного воздействия на нарушения и заболевания с использованием вирусных компонентов |
EP4245853A3 (en) | 2013-06-17 | 2023-10-18 | The Broad Institute, Inc. | Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation |
CA2915834A1 (en) | 2013-06-17 | 2014-12-24 | Massachusetts Institute Of Technology | Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation |
WO2015065964A1 (en) | 2013-10-28 | 2015-05-07 | The Broad Institute Inc. | Functional genomics using crispr-cas systems, compositions, methods, screens and applications thereof |
CN106061510B (zh) | 2013-12-12 | 2020-02-14 | 布罗德研究所有限公司 | 用于基因组编辑的crispr-cas系统和组合物的递送、用途和治疗应用 |
WO2015089364A1 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Crystal structure of a crispr-cas system, and uses thereof |
KR20160097331A (ko) | 2013-12-12 | 2016-08-17 | 더 브로드 인스티튜트, 인코퍼레이티드 | 뉴클레오티드 반복 장애에서의 crispr-cas 시스템의 조성물 및 방법 및 용도 |
EP3080271B1 (en) | 2013-12-12 | 2020-02-12 | The Broad Institute, Inc. | Systems, methods and compositions for sequence manipulation with optimized functional crispr-cas systems |
CA2932439A1 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute, Inc. | Crispr-cas systems and methods for altering expression of gene products, structural information and inducible modular cas enzymes |
WO2016094867A1 (en) | 2014-12-12 | 2016-06-16 | The Broad Institute Inc. | Protected guide rnas (pgrnas) |
WO2016205759A1 (en) | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation |
TWI813532B (zh) | 2015-06-18 | 2023-09-01 | 美商博得學院股份有限公司 | 降低脱靶效應的crispr酶突變 |
EP3814522A4 (en) * | 2018-06-29 | 2022-03-23 | Seegene, Inc. | METHOD OF PREDICTING THE MELTING TEMPERATURE OF AN OLIGONUCLEOTIDE |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3756313B2 (ja) | 1997-03-07 | 2006-03-15 | 武 今西 | 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体 |
US6794499B2 (en) | 1997-09-12 | 2004-09-21 | Exiqon A/S | Oligonucleotide analogues |
US7715989B2 (en) * | 1998-04-03 | 2010-05-11 | Elitech Holding B.V. | Systems and methods for predicting oligonucleotide melting temperature (TmS) |
US6734291B2 (en) | 1999-03-24 | 2004-05-11 | Exiqon A/S | Synthesis of [2.2.1]bicyclo nucleosides |
EP1882748A3 (en) | 2002-09-11 | 2008-06-25 | Exiqon A/S | A population of nucleic acids including a subpopulation of LNA oligomers |
US6889143B2 (en) | 2002-09-12 | 2005-05-03 | Intergrated Dna Technologies, Inc. | Methods and systems for estimating the melting temperature (TM) for polynucleotide molecules |
US20040219565A1 (en) * | 2002-10-21 | 2004-11-04 | Sakari Kauppinen | Oligonucleotides useful for detecting and analyzing nucleic acids of interest |
CA2640171C (en) | 2006-01-27 | 2014-10-28 | Isis Pharmaceuticals, Inc. | 6-modified bicyclic nucleic acid analogs |
US7569686B1 (en) | 2006-01-27 | 2009-08-04 | Isis Pharmaceuticals, Inc. | Compounds and methods for synthesis of bicyclic nucleic acid analogs |
-
2011
- 2011-07-29 WO PCT/US2011/046010 patent/WO2012018697A1/en active Application Filing
- 2011-07-29 EP EP11749613.3A patent/EP2601611B1/en active Active
- 2011-07-29 US US13/194,790 patent/US9081737B2/en active Active
- 2011-07-29 DK DK11749613.3T patent/DK2601611T3/da active
Also Published As
Publication number | Publication date |
---|---|
EP2601611A1 (en) | 2013-06-12 |
US20120029891A1 (en) | 2012-02-02 |
EP2601611B1 (en) | 2020-12-09 |
US9081737B2 (en) | 2015-07-14 |
WO2012018697A1 (en) | 2012-02-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DK2601611T3 (da) | Fremgangsmåder til forudsigelse af stabilitet og smeltetemperaturer for nukleinsyreduplekser | |
DK3012329T3 (da) | Fremgangsmåder og materialer til at vurdere tab af heterozygositet | |
DK2912174T3 (da) | Fremgangsmåde og materialer til isolering af nukleinsyrematerialer | |
DK2794907T3 (da) | Fremgangsmåder og materialer til vurdering af tab af heterozygositet | |
DK2539450T3 (da) | Fremgangsmåde til fremstilling af nukleinsyrebiblioteker | |
DK3109326T3 (da) | Sammensætning og fremgangsmåde til nukleinsyresyntese og amplifikation under anvendelse af rt | |
DK2643782T3 (da) | Systemer og fremgangsmåder til bedømmelse af kognitiv funktion | |
DK2836609T4 (da) | Sammensætninger og fremgangsmåder til kvantificering af en nucleinsyresekvens i en prøve | |
DK2625253T3 (da) | System og fremgangsmåde til ristning af biomasse | |
DK2831276T3 (da) | Sammensætninger og fremgangsmåde til at måle og kalibrere amplifikations-bias i multipleks-PCR-reaktioner | |
DK2914741T3 (da) | Hidtil ukendte sammensætninger og fremgangsmåder til forbedring af PCR-specificitet | |
DK3597749T3 (da) | Fremgangsmåder og sammensætninger til rna-dirigeret mål-dna-modificering og til rna-dirigeret modulering af transkription | |
DK3428290T3 (da) | Sammensætninger og fremgangsmåder til amplifikation af nukleinsyrer | |
DK3290528T3 (da) | Fremgangsmåder og sammensætninger til nukleinsyresekventering | |
DK2971167T3 (da) | Sammensætninger og fremgangsmåder til targeting af nucleinsyre med nucleinsyrer | |
DK2809795T3 (da) | Materialer og fremgangsmåder til syntese af fejlminimerede nukleinsyremolekyler | |
DK2181196T3 (da) | Nicking- og forlængelsesamplifikationsreaktioner til eksponentiel amplificering af nucleinsyrer | |
DK2183753T3 (da) | Fremgangsmåder til køling | |
DK2591233T3 (da) | System og fremgangsmåde til håndtering af vindmølletårnsektioner | |
DK3594340T3 (da) | Sammensætninger og fremgangsmåder til samling med høj nøjagtighed af nukleinsyrer | |
DK2497834T4 (da) | Fremgangsmåde til påvisning af methyleringen af colon-cancer-specifikke methyleringsmarkørgener til colon-cancer-diagnose | |
DK3363901T3 (da) | Sammensætninger og fremgangsmåder til præcis identificering af mutationer | |
DK2838917T3 (da) | Fremgangsmåder og midler til frembringelse af heterodimere ig-lignende molekyler | |
DK2598500T3 (da) | Sammensætninger og fremgangsmåder til hæmning af jak-reaktionsvejen | |
DK3375891T3 (da) | Fremgangsmåder og indretninger til kompensation af spektral krydstale i multipleks-nukleinsyreamplifikation |