SG10201801188XA - Selective delivery of material to cells - Google Patents

Selective delivery of material to cells

Info

Publication number
SG10201801188XA
SG10201801188XA SG10201801188XA SG10201801188XA SG10201801188XA SG 10201801188X A SG10201801188X A SG 10201801188XA SG 10201801188X A SG10201801188X A SG 10201801188XA SG 10201801188X A SG10201801188X A SG 10201801188XA SG 10201801188X A SG10201801188X A SG 10201801188XA
Authority
SG
Singapore
Prior art keywords
cells
selective delivery
selective
delivery
Prior art date
Application number
SG10201801188XA
Other languages
English (en)
Inventor
Armon R Sharei
Viktor A Adalsteinsson
Nahyun Cho
Robert S Langer
J Love
Klavs Jensen
Original Assignee
Massachusetts Inst Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Inst Technology filed Critical Massachusetts Inst Technology
Publication of SG10201801188XA publication Critical patent/SG10201801188XA/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1484—Electro-optical investigation, e.g. flow cytometers microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • G01N15/01—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G01N2015/1028—

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Dispersion Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Oncology (AREA)
  • Fluid Mechanics (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Hospice & Palliative Care (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Medicinal Chemistry (AREA)
  • Nanotechnology (AREA)
  • Composite Materials (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Materials Engineering (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
SG10201801188XA 2013-08-16 2014-08-15 Selective delivery of material to cells SG10201801188XA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201361866972P 2013-08-16 2013-08-16

Publications (1)

Publication Number Publication Date
SG10201801188XA true SG10201801188XA (en) 2018-03-28

Family

ID=52468732

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201801188XA SG10201801188XA (en) 2013-08-16 2014-08-15 Selective delivery of material to cells
SG11201601927SA SG11201601927SA (en) 2013-08-16 2014-08-15 Selective delivery of material to cells

Family Applications After (1)

Application Number Title Priority Date Filing Date
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Country Status (13)

Country Link
US (3) US10124336B2 (zh)
EP (2) EP3848695A1 (zh)
JP (1) JP6502940B2 (zh)
KR (4) KR102530914B1 (zh)
CN (1) CN105848793B (zh)
AU (2) AU2014306423B2 (zh)
CA (1) CA2921579C (zh)
ES (1) ES2865107T3 (zh)
IL (1) IL244113B (zh)
MX (2) MX2016002058A (zh)
PL (1) PL3033184T3 (zh)
SG (2) SG10201801188XA (zh)
WO (1) WO2015023982A1 (zh)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RS59898B1 (sr) 2011-10-17 2020-03-31 Massachusetts Inst Technology Intraćelijsko davanje
AU2014306423B2 (en) 2013-08-16 2019-04-18 Massachusetts Institute Of Technology Selective delivery of material to cells
CN107109362A (zh) * 2014-10-31 2017-08-29 麻省理工学院 递送生物分子至免疫细胞
US10526573B2 (en) 2014-11-14 2020-01-07 Massachusetts Institute Of Technology Disruption and field enabled delivery of compounds and compositions into cells
JP7278027B2 (ja) 2015-01-12 2023-05-19 マサチューセッツ インスティテュート オブ テクノロジー マイクロ流体送達による遺伝子編集
EP3320082B1 (en) 2015-07-09 2023-05-24 Massachusetts Institute of Technology Delivery of materials to anucleate cells
US11613759B2 (en) 2015-09-04 2023-03-28 Sqz Biotechnologies Company Intracellular delivery of biomolecules to cells comprising a cell wall
ES2930017T3 (es) 2015-09-04 2022-12-05 Sqz Biotechnologies Co Suministro intracelular de biomoléculas mediado por una superficie con poros
WO2017070169A1 (en) * 2015-10-19 2017-04-27 The Methodist Hospital Crispr-cas9 delivery to hard-to-transfect cells via membrane deformation
JP2019500043A (ja) 2015-12-28 2019-01-10 ノバルティス アーゲー 異常ヘモグロビン症の治療用組成物および方法
US20190017072A1 (en) 2016-01-12 2019-01-17 Sqz Biotechnologies Company Intracellular delivery of complexes
US20190275520A1 (en) 2016-03-31 2019-09-12 Massachusetts Institute Of Technology Flow-through microfluidic methods and devices featuring membrane-perturbing surface interactions for intracellular delivery
RU2770492C2 (ru) * 2016-05-03 2022-04-18 ЭсКьюЗед БАЙОТЕКНОЛОДЖИЗ КОМПАНИ Внутриклеточная доставка биологических молекул для индуцирования толерантности
KR102490952B1 (ko) 2016-05-03 2023-01-19 에스큐지 바이오테크놀로지스 컴퍼니 관용을 유도하는 생체분자의 세포내 전달
CN106378213B (zh) * 2016-05-05 2018-08-17 海南大学 基于介电泳的可变形微颗粒分离芯片
JP7084393B2 (ja) * 2016-11-08 2022-06-14 ジョージア テック リサーチ コーポレイション 対流駆動細胞内輸送のための方法
TW201839136A (zh) 2017-02-06 2018-11-01 瑞士商諾華公司 治療血色素異常症之組合物及方法
US11383241B2 (en) * 2017-10-11 2022-07-12 The Regents Of The University Of California Mechano-node pore sensing
WO2019089034A1 (en) * 2017-11-02 2019-05-09 The Methodist Hospital Crispr-cas9 delivery to hard-to-transfect cells via membrane deformation
CN111683964A (zh) 2017-12-05 2020-09-18 Sqz生物技术公司 细胞内递送生物分子来调节抗体产生
WO2019126146A1 (en) 2017-12-19 2019-06-27 Xcell Biosciences, Inc. Methods of modulating cell phenotype by way of regulating the gaseous environment
EP3728559A1 (en) 2017-12-20 2020-10-28 SQZ Biotechnologies Company System for delivery of a payload into a cell
JP2019154314A (ja) * 2018-03-13 2019-09-19 国立大学法人九州大学 キャピラリーチップ及びこれを備える装置、並びに細胞への外来物質の導入方法
EP3556845A1 (en) 2018-04-20 2019-10-23 Cellix Limited A method and device for transfecting cells
CN109351370B (zh) * 2018-11-21 2020-05-05 晶准生物医学（深圳）有限公司 微流控芯片和细胞筛选方法
KR20210121106A (ko) 2019-01-25 2021-10-07 에스큐지 바이오테크놀로지스 컴퍼니 무핵 세포-유래된 백신
AU2020228648A1 (en) 2019-02-28 2021-10-14 Sqz Biotechnologies Company Delivery of biomolecules to PBMCs to modify an immune response
DE102019108155B3 (de) 2019-03-29 2020-06-04 Leibniz-Institut Für Photonische Technologien E.V. Mikrotropfenrückhalteanordnung
US11679388B2 (en) 2019-04-08 2023-06-20 Sqz Biotechnologies Company Cartridge for use in a system for delivery of a payload into a cell
WO2020207456A1 (en) * 2019-04-11 2020-10-15 The Chinese University Of Hong Kong Systems and methods for controlled membrane disruption
WO2020232418A1 (en) * 2019-05-15 2020-11-19 Cellfe, Inc. Intracellular delivery
WO2021123920A1 (en) 2019-12-18 2021-06-24 Novartis Ag Compositions and methods for the treatment of hemoglobinopathies
CN111413257B (zh) * 2020-01-21 2021-05-14 中国科学院电子学研究所 细胞核电学性能检测装置及方法
TW202207975A (zh) 2020-05-11 2022-03-01 瑞士商赫孚孟拉羅股份公司 使用經修飾之ｐｂｍｃ及免疫結合體之組合療法
CN116348583A (zh) * 2020-05-26 2023-06-27 川赛托斯有限责任公司 用于转染的装置和方法
WO2022026620A1 (en) 2020-07-29 2022-02-03 Sqz Biotechnologies Company Methods to stimulate immune responses to mutant ras using nucleated cells
CN116322752A (zh) 2020-07-29 2023-06-23 Sqz生物技术公司 使用无核细胞刺激对突变体ras的免疫应答的方法
IL302976A (en) 2020-11-18 2023-07-01 Cellfe Inc Mechanoporation-based methods and systems for high-throughput charge transfer into biological cells
CN116801719A (zh) 2020-12-29 2023-09-22 Sqz生物技术公司 用于pbmc冷冻保存的调配物
CA3203356A1 (en) 2020-12-29 2022-07-07 Oliver Rosen Methods for treating cancers with activating antigen carriers
EP4271793A1 (en) 2020-12-29 2023-11-08 SQZ Biotechnologies Company Microfluidic chip having increased throughput for use in a system for delivery of a payload into a cell
US20220233677A1 (en) 2020-12-29 2022-07-28 Sqz Biotechnologies Company Methods for treating cancers with modified pbmcs
CN116847880A (zh) 2020-12-29 2023-10-03 Sqz生物技术公司 活化抗原载体的调配物
JP2024502791A (ja) 2020-12-29 2024-01-23 スクイーズ バイオテクノロジーズ カンパニー 細胞膜を摂動させるための並列化された狭窄部を有する高スループットマイクロ流体チップ
EP4359527A2 (en) 2021-06-23 2024-05-01 Novartis AG Compositions and methods for the treatment of hemoglobinopathies
WO2023010090A1 (en) 2021-07-29 2023-02-02 Sqz Biotechnologies Company Methods to generate enhanced tumor infiltrating lymphocytes through microfluidic delivery
WO2023087009A1 (en) 2021-11-11 2023-05-19 Sqz Biotechnologies Company Methods to generate enhanced tumor infiltrating lymphocytes through microfluidic delivery
WO2023230253A1 (en) 2022-05-27 2023-11-30 Sqz Biotechnologies Company Cartridges and devices for use in a system for delivery of a payload into a cell
WO2024026491A2 (en) 2022-07-28 2024-02-01 Sqz Biotechnologies Company Enhanced antigen presenting cell formulations
WO2024026492A1 (en) 2022-07-28 2024-02-01 Sqz Biotechnologies Company Methods for treating cancer with enhanced antigen presenting cells

Family Cites Families (123)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2502621C3 (de) 1975-01-23 1978-09-14 Kernforschungsanlage Juelich Gmbh, 5170 Juelich Messung elastischer und dielektrischer Eigenschaften der Membran lebender Zellen
US4376634A (en) 1980-05-30 1983-03-15 Mallinckrodt, Inc. Assay kit having syringe, dilution device and reagents within sealed container
US4478824A (en) 1983-08-08 1984-10-23 Franco Robert S Method for altering red blood cell function and survival
FR2569477B1 (fr) 1984-08-24 1987-01-02 Descartes Universite Rene Appareil et procede pour la determination de la deformabilite des globules rouges du sang
JP2720161B2 (ja) 1988-02-01 1998-02-25 株式会社アドバンス 細胞変形能測定装置
JP2685544B2 (ja) 1988-11-11 1997-12-03 株式会社日立製作所 血液フィルタおよび血液検査方法並びに血液検査装置
JP2532707B2 (ja) 1990-03-08 1996-09-11 佑二 菊池 血液回路及びこれを用いた血液測定装置及び血液測定方法
WO1991016073A1 (en) 1990-04-24 1991-10-31 The University Of Newcastle Research Associates Limited Oral vaccine comprising antigen surface-associated with red blood cells
US5658892A (en) 1993-01-15 1997-08-19 The General Hospital Corporation Compound delivery using high-pressure impulse transients
US6218166B1 (en) 1994-12-09 2001-04-17 John Wayne Cancer Institute Adjuvant incorporation into antigen carrying cells: compositions and methods
ATE240119T1 (de) 1995-03-08 2003-05-15 Scripps Research Inst Antigen präsentierendes system und aktivierung von t-zellen
US6051409A (en) 1995-09-25 2000-04-18 Novartis Finance Corporation Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells
US6133503A (en) 1995-10-31 2000-10-17 The Regents Of The University Of California Mammalian artificial chromosomes and methods of using same
AU725669B2 (en) 1995-12-04 2000-10-19 Puget Sound Blood Center Non-immunogenic and toleragenic platelet and red blood cell compositions
WO1997035619A1 (en) 1996-03-28 1997-10-02 Genitrix, L.L.C. Opsonin-enhanced cells, and methods of modulating an immune response to an antigen
US5885470A (en) 1997-04-14 1999-03-23 Caliper Technologies Corporation Controlled fluid transport in microfabricated polymeric substrates
EP0882448B1 (en) 1997-05-05 2005-01-12 DIDECO S.r.l. Method of encapsulating biologically active agents within erythrocytes and apparatus therefor
US5842787A (en) 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
SE9704076D0 (sv) 1997-11-06 1997-11-06 Holdingbolaget Vid Goeteborgs Method for permeabilisation of cell structures and use thereof
GB9816583D0 (en) 1998-07-31 1998-09-30 Univ Ulster Nucleic acid carrier
US6562616B1 (en) 1999-06-21 2003-05-13 The General Hospital Corporation Methods and devices for cell culturing and organ assist systems
JP2002325572A (ja) 2000-12-25 2002-11-12 Univ Osaka 外来物質の導入方法
WO2002067863A2 (en) 2001-02-22 2002-09-06 The Scepens Eye Research Institute, Inc. Tolerogenic antigen presenting cells and in treating immune-inflammatory conditions
WO2003020039A1 (en) 2001-08-28 2003-03-13 Rush-Presbyterian-St. Luke's Medical Center Immune tolerance to predetermined antigens
JP4842512B2 (ja) 2001-11-27 2011-12-21 セレクトリコン アーベー 並列薬剤送達と細胞構造の電気穿孔法を併合した方法及びその使用
US20030133922A1 (en) 2002-01-15 2003-07-17 Kasha John R. Oral tolerance using allogeneic platelets in ITP
WO2003104788A1 (ja) 2002-06-05 2003-12-18 松下電器産業株式会社 細胞外電位測定デバイスおよびその製造方法
GB0214528D0 (en) 2002-06-24 2002-08-07 Univ Aberdeen Materials and methods for induction of immune tolerance
CA2513072A1 (en) 2003-01-09 2004-07-29 Invitrogen Corporation Cellular delivery and activation polypeptide-nucleic acid complexes
US20060134067A1 (en) 2003-02-18 2006-06-22 Maxcyte, Inc. Loading of cells with antigens by electroporation
WO2005123905A1 (ja) 2004-06-17 2005-12-29 Ken Nakata 生体力学的刺激負荷による細胞培養方法及びその装置
WO2006010521A1 (en) 2004-07-27 2006-02-02 Dsm Ip Assets B.V. Process for making a carbon nanotubes / ultra-high molar mass polyethylene composite fibre
FR2873925B1 (fr) 2004-08-05 2006-10-13 Erytech Pharma Soc Par Actions Procede et dispositif de lyse-rescellement pour l'incorporation de principe actif notamment asparaginase ou inositol hexaphosphate, dans des erythrocytes
ES2350895T3 (es) 2004-08-19 2011-01-28 University College Cardiff Consultants Limited Preparación de células t gamma delta humanas presentadoras de antígeno y su uso en inmunoterapia.
US20060134772A1 (en) 2004-11-18 2006-06-22 The Regents Of The University Of California System for locating cells and for cellular analysis
WO2006095330A2 (en) 2005-03-10 2006-09-14 Yeda Research And Development Co. Ltd. Methods and immunogenic cell preparations for treating antigen-associated diseases
US7704743B2 (en) 2005-03-30 2010-04-27 Georgia Tech Research Corporation Electrosonic cell manipulation device and method of use thereof
KR100760309B1 (ko) 2005-07-06 2007-10-05 한국과학기술원 미소필터를 이용한 미소입자 변형성 분석기
US7993821B2 (en) 2005-08-11 2011-08-09 University Of Washington Methods and apparatus for the isolation and enrichment of circulating tumor cells
US9005974B2 (en) 2005-12-09 2015-04-14 Academish Medisch Centrum Bij De Universiteit Van Amsterdam Means and methods for influencing the stability of cells
WO2007097934A2 (en) 2006-02-17 2007-08-30 Elusys Therapeutics, Inc. Methods and compositions for using erythrocytes as carriers for delivery of drugs
US8293524B2 (en) 2006-03-31 2012-10-23 Fluxion Biosciences Inc. Methods and apparatus for the manipulation of particle suspensions and testing thereof
US20070249038A1 (en) 2006-04-21 2007-10-25 Andrea Adamo Microfluidic device for single cell targeted operations
EP2054499A2 (en) 2006-08-17 2009-05-06 Massachusetts Institute of Technology Method and apparatus for microfluidic injection
US20080241844A1 (en) 2007-02-06 2008-10-02 Network Biosystems, Inc. Devices and Methods for the Performance of Miniaturized In Vitro Assays
US20080311140A1 (en) 2007-05-29 2008-12-18 Baylor College Of Medicine Antigen specific immunosuppression by dendritic cell therapy
US8841135B2 (en) 2007-06-20 2014-09-23 University Of Washington Biochip for high-throughput screening of circulating tumor cells
WO2008156021A1 (ja) 2007-06-21 2008-12-24 National University Corporation Nagoya University 細胞壁を備える細胞に外来物質を導入する方法
FR2919804B1 (fr) 2007-08-08 2010-08-27 Erytech Pharma Composition et vaccin therapeutique anti-tumoral
GB0718160D0 (en) 2007-09-18 2007-10-24 Medical Res Council Methods
EP2057998A1 (en) 2007-10-31 2009-05-13 Universitätsklinikum Hamburg-Eppendorf Use of modified cells for the treatment of multiple sclerosis
KR100891487B1 (ko) 2007-11-01 2009-04-01 한국과학기술원 미세유체제어기술 기반으로 한 난모세포 또는 난자의 핵제거 시스템
EP2216639A4 (en) 2007-11-28 2014-01-15 Konica Minolta Opto Inc APPARATUS AND METHOD FOR MEASURING BLOOD FLUIDITY
US20090280518A1 (en) 2008-05-12 2009-11-12 Massachusetts Institute Of Technology System for high throughput measurement of mechanical properties of cells
JP5137129B2 (ja) 2008-07-23 2013-02-06 国立大学法人山口大学 血液細胞の力学的特性測定装置
EP2321405A4 (en) 2008-08-08 2012-09-12 Agency Science Tech & Res MICROFLUIDIC CONTINUOUS FLOW DEVICE
US8211656B2 (en) 2008-08-13 2012-07-03 The Invention Science Fund I, Llc Biological targeting compositions and methods of using the same
US20110091973A1 (en) 2008-12-09 2011-04-21 Glaser Larry F Modified and fusion enhanced erythrocytes, cells and uses thereof
US9157550B2 (en) 2009-01-05 2015-10-13 The Board Of Trustees Of The University Of Illinois Microfluidic systems and methods
EP2405959A4 (en) 2009-03-13 2013-10-16 Univ Tufts METHODS, APPARATUSES AND KITS FOR INTRODUCING GENETIC MATERIAL INTO LIVING CELLS
US9017991B2 (en) 2009-03-13 2015-04-28 Tufts University Methods tip assemblies and kits for introducing material into cells
WO2010107399A1 (en) 2009-03-20 2010-09-23 Agency For Science, Technology And Research Devices for separating cells and methods of using them
JP4849278B2 (ja) * 2009-03-27 2012-01-11 セイコーエプソン株式会社 細胞処理デバイス、細胞処理カートリッジおよび体液処理システム
WO2010129671A2 (en) 2009-05-08 2010-11-11 Chauncey Sayre Method of and apparatus for changing one type of cell into another type of cell
GB0909754D0 (en) 2009-06-05 2009-07-22 Magnani Mauro Drug delivery systems
AU2010266705B2 (en) 2009-06-30 2014-06-05 Sangamo Therapeutics, Inc. Rapid screening of biologically active nucleases and isolation of nuclease-modified cells
EP2451475A2 (en) 2009-07-06 2012-05-16 Novartis AG Self replicating rna molecules and uses thereof
KR101125060B1 (ko) * 2009-07-22 2012-03-21 한국과학기술원 입자를 포획하는 미세유체 소자 및 이를 이용한 입자 포획 방법
US9364831B2 (en) 2009-08-08 2016-06-14 The Regents Of The University Of California Pulsed laser triggered high speed microfluidic switch and applications in fluorescent activated cell sorting
US20120207745A1 (en) 2009-10-27 2012-08-16 Yann Godfrin Composition to Induce Specific Immune Tolerance
WO2011078665A1 (en) 2009-12-21 2011-06-30 Keygene N.V. Improved techniques for transfecting protoplasts
EP2516320A4 (en) * 2009-12-23 2015-03-04 Cytovera Inc PARTICULATE FILTERING SYSTEM AND METHOD
WO2011084882A2 (en) 2010-01-05 2011-07-14 Contrafect Corporation Methods and compositions for enhanced immunological therapy and targeting of gram-positive bacteria
US8844570B2 (en) 2010-02-01 2014-09-30 California Institute Of Technology Generating binary states using a microfluidic channel
JP5704590B2 (ja) 2010-02-05 2015-04-22 国立大学法人東京農工大学 サイズ選択マイクロキャビティアレイを用いた循環腫瘍細胞の検出
CN107604039B (zh) 2010-03-04 2022-04-15 新加坡国立大学 检测和分离细胞的微流体分选器
US20120064505A1 (en) 2010-03-22 2012-03-15 Massachusetts Institute Of Technology Measurement of material properties and related methods and compositions
CN103221070B (zh) 2010-08-30 2019-07-12 哈佛大学校长及研究员协会 用于狭窄病变和溶解血栓疗法的切变控制释放
PL2643345T3 (pl) 2010-11-25 2021-08-16 Imnate Sarl Immunogenne peptydy do zastosowania w zapobieganiu i/lub leczeniu chorób zakaźnych, chorób autoimmunologicznych, odpowiedzi immunologicznych na czynniki allogeniczne, chorób alergicznych, nowotworów, odrzuceniu przeszczepów oraz odpowiedzi immunologicznych skierowanych przeciwko wektorom wirusowym stosowanym w terapii genowej lub szczepieniu genowym
US20140034556A1 (en) 2011-01-19 2014-02-06 The University Of British Columbia Apparatus and method for particle separation
MX363013B (es) 2011-02-25 2019-03-04 Recombinetics Inc Animales genéticamente modificados y métodos para su obtención.
KR101957923B1 (ko) 2011-02-28 2019-03-14 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 세포 배양 시스템
CN108531360A (zh) 2011-05-27 2018-09-14 不列颠哥伦比亚大学 用于高通量分析的微流控细胞捕获和分析设备
MX2013013243A (es) 2011-07-05 2014-05-30 Sotio As Medios y metodos para activar la inmunoterapia celular del cancer utilizando celulas cancerosas destruidas mediante presion hidrostatica y celulas dendriticas.
US20130065314A1 (en) 2011-08-25 2013-03-14 Phytocentric Biotech Inc. Algal transformation systems, compositions and methods
RS59898B1 (sr) 2011-10-17 2020-03-31 Massachusetts Inst Technology Intraćelijsko davanje
EP2858630A4 (en) 2012-06-07 2016-02-24 Harvard College NANOTHERAPEUTIC PRODUCTS FOR DRUG TARGETING
US9255245B2 (en) 2012-07-03 2016-02-09 Agilent Technologies, Inc. Sample probes and methods for sampling intracellular material
DE202013012597U1 (de) 2012-10-23 2017-11-21 Toolgen, Inc. Zusammensetzung zum Spalten einer Ziel-DNA, umfassend eine für die Ziel-DNA spezifische guide-RNA und eine Cas-Protein-codierende Nukleinsäure oder ein Cas-Protein, sowie deren Verwendung
CN102925337B (zh) 2012-11-08 2014-06-18 武汉友芝友生物制药有限公司 一种微流体细胞捕获芯片及其制备方法
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
GB201300052D0 (en) 2013-01-03 2013-02-20 Transimmune Ag Method for obtaining immuno-suppressive dendritic cells
GB201300049D0 (en) 2013-01-03 2013-02-20 Transimmune Ag Method for obtaining immuno-stimulatory dendritic cells
WO2014120956A1 (en) 2013-01-30 2014-08-07 Asta Fluidic Technologies, Inc. Microfluidic-based fetal red blood cell detection
US9725709B2 (en) 2013-03-12 2017-08-08 OpenCell Technologies, Inc. Intracellular delivery and transfection methods and devices
AU2014236747B2 (en) 2013-03-15 2019-11-07 The Regents Of The University Of California High-throughput cargo delivery into live cells using photothermal platforms
KR20140115560A (ko) 2013-03-21 2014-10-01 인하대학교 산학협력단 기계적 스트레스를 이용한 단세포 생물체 개질 방법 및 이에 사용되는 장치
EP3789487A1 (en) 2013-04-03 2021-03-10 Memorial Sloan Kettering Cancer Center Effective generation of tumor-targeted t-cells derived from pluripotent stem cells
KR101508974B1 (ko) * 2013-05-14 2015-04-07 한국과학기술연구원 종양 세포의 검출장치 및 검출방법
AU2014306423B2 (en) 2013-08-16 2019-04-18 Massachusetts Institute Of Technology Selective delivery of material to cells
EP3060648A4 (en) 2013-10-22 2017-05-31 Cellanyx Diagnostics, LLC Systems, devices and methods for microfluidic culturing, manipulation and analysis of tissues and cells
WO2015103331A1 (en) 2013-12-31 2015-07-09 Canon U.S. Life Sciences, Inc. Methods and systems for continuous flow cell lysis in a microfluidic device
RU2736495C2 (ru) 2014-04-01 2020-11-17 Рубиус Терапьютикс, Инк. Способ и композиции для иммуномодуляции
KR20230152175A (ko) 2014-04-18 2023-11-02 에디타스 메디신, 인코포레이티드 암 면역요법을 위한 crispr-cas-관련 방법, 조성물 및 구성성분
WO2016003485A1 (en) 2014-07-03 2016-01-07 Massachusetts Institute Of Technology Microfluidic assay for rapid optimization of cell electroporation
CN107109362A (zh) 2014-10-31 2017-08-29 麻省理工学院 递送生物分子至免疫细胞
US10526573B2 (en) 2014-11-14 2020-01-07 Massachusetts Institute Of Technology Disruption and field enabled delivery of compounds and compositions into cells
WO2016094715A2 (en) 2014-12-10 2016-06-16 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
AU2015376652B2 (en) 2015-01-07 2017-11-30 Indee. Inc. A method for mechanical and hydrodynamic microfluidic transfection and apparatus therefor
JP7278027B2 (ja) 2015-01-12 2023-05-19 マサチューセッツ インスティテュート オブ テクノロジー マイクロ流体送達による遺伝子編集
US20180135012A1 (en) 2015-05-13 2018-05-17 Rubius Therapeutics, Inc. Membrane-receiver complex therapeutics
GB201513278D0 (en) 2015-07-03 2015-09-09 Transimmune Ag And Yale University Device and method for obtaining immunostimulatory antigen-presenting cells
EP3320082B1 (en) 2015-07-09 2023-05-24 Massachusetts Institute of Technology Delivery of materials to anucleate cells
ES2930017T3 (es) 2015-09-04 2022-12-05 Sqz Biotechnologies Co Suministro intracelular de biomoléculas mediado por una superficie con poros
US11613759B2 (en) 2015-09-04 2023-03-28 Sqz Biotechnologies Company Intracellular delivery of biomolecules to cells comprising a cell wall
EP3393482A4 (en) 2015-12-22 2019-08-21 Sangui Bio Pty. Ltd THERAPEUTIC PROCEDURES USING ERYTHROCYTES
LT3402491T (lt) 2016-01-11 2022-02-25 Rubius Therapeutics, Inc. Kompozicijos ir būdai, susiję su daugiamodalinėmis terapinėmis ląstelių sistemomis, skirti vėžio indikacijoms
US20190017072A1 (en) 2016-01-12 2019-01-17 Sqz Biotechnologies Company Intracellular delivery of complexes
KR102490952B1 (ko) 2016-05-03 2023-01-19 에스큐지 바이오테크놀로지스 컴퍼니 관용을 유도하는 생체분자의 세포내 전달
RU2770492C2 (ru) 2016-05-03 2022-04-18 ЭсКьюЗед БАЙОТЕКНОЛОДЖИЗ КОМПАНИ Внутриклеточная доставка биологических молекул для индуцирования толерантности
US20170326213A1 (en) 2016-05-16 2017-11-16 Augusta University Research Institute, Inc. Protein-Coupled Red Blood Cell Compositions and Methods of Their Use
CN106244543A (zh) 2016-07-29 2016-12-21 北京时合生物科技有限公司 一种pbmc体外诱导分化成为树突状细胞的方法
JP7084393B2 (ja) 2016-11-08 2022-06-14 ジョージア テック リサーチ コーポレイション 対流駆動細胞内輸送のための方法

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