PL3033184T3 - Selektywne dostarczanie materiału do komórek - Google Patents

Selektywne dostarczanie materiału do komórek

Info

Publication number
PL3033184T3
PL3033184T3 PL14836593T PL14836593T PL3033184T3 PL 3033184 T3 PL3033184 T3 PL 3033184T3 PL 14836593 T PL14836593 T PL 14836593T PL 14836593 T PL14836593 T PL 14836593T PL 3033184 T3 PL3033184 T3 PL 3033184T3
Authority
PL
Poland
Prior art keywords
cells
selective delivery
selective
delivery
Prior art date
Application number
PL14836593T
Other languages
English (en)
Inventor
Armon R. Sharei
Viktor A. Adalsteinsson
Nahyun CHO
Robert S. Langer
J. Christopher Love
Klavs F. Jensen
Original Assignee
Massachusetts Institute Of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Institute Of Technology filed Critical Massachusetts Institute Of Technology
Publication of PL3033184T3 publication Critical patent/PL3033184T3/pl

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1028—Sorting particles

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Dispersion Chemistry (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Oncology (AREA)
  • Biophysics (AREA)
  • Nanotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Hospice & Palliative Care (AREA)
  • Fluid Mechanics (AREA)
  • Toxicology (AREA)
  • Composite Materials (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Materials Engineering (AREA)
  • Crystallography & Structural Chemistry (AREA)
PL14836593T 2013-08-16 2014-08-15 Selektywne dostarczanie materiału do komórek PL3033184T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361866972P 2013-08-16 2013-08-16
EP14836593.5A EP3033184B1 (en) 2013-08-16 2014-08-15 Selective delivery of material to cells
PCT/US2014/051343 WO2015023982A1 (en) 2013-08-16 2014-08-15 Selective delivery of material to cells

Publications (1)

Publication Number Publication Date
PL3033184T3 true PL3033184T3 (pl) 2021-09-20

Family

ID=52468732

Family Applications (1)

Application Number Title Priority Date Filing Date
PL14836593T PL3033184T3 (pl) 2013-08-16 2014-08-15 Selektywne dostarczanie materiału do komórek

Country Status (13)

Country Link
US (3) US10124336B2 (pl)
EP (2) EP3848695A1 (pl)
JP (1) JP6502940B2 (pl)
KR (4) KR102386122B1 (pl)
CN (1) CN105848793B (pl)
AU (2) AU2014306423B2 (pl)
CA (1) CA2921579C (pl)
ES (1) ES2865107T3 (pl)
IL (1) IL244113B (pl)
MX (2) MX382654B (pl)
PL (1) PL3033184T3 (pl)
SG (2) SG10201801188XA (pl)
WO (1) WO2015023982A1 (pl)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107058101B (zh) 2011-10-17 2021-06-01 麻省理工学院 细胞内传递
KR102386122B1 (ko) 2013-08-16 2022-04-14 메사추세츠 인스티튜트 오브 테크놀로지 세포로의 선택적 물질 전달
CA2964392A1 (en) * 2014-10-31 2016-05-06 Massachussetts Institute Of Technology Delivery of biomolecules to immune cells
CA2964138C (en) 2014-11-14 2023-11-14 Massachusetts Institute Of Technology Disruption and field enabled delivery of compounds and compositions into cells
AU2016206870B2 (en) 2015-01-12 2022-02-17 Massachusetts Institute Of Technology Gene editing through microfluidic delivery
CA2988996A1 (en) 2015-07-09 2017-01-12 Massachusetts Institute Of Technology Delivery of materials to anucleate cells
EP3344575B1 (en) 2015-09-04 2020-04-15 SQZ Biotechnologies Company Intracellular delivery of biomolecules to cells comprising a cell wall
ES2930017T3 (es) 2015-09-04 2022-12-05 Sqz Biotechnologies Co Suministro intracelular de biomoléculas mediado por una superficie con poros
WO2017070169A1 (en) * 2015-10-19 2017-04-27 The Methodist Hospital Crispr-cas9 delivery to hard-to-transfect cells via membrane deformation
NZ783532A (en) 2015-12-28 2025-09-26 Novartis Ag Compositions and methods for the treatment of hemoglobinopathies
JP7033535B2 (ja) 2016-01-12 2022-03-10 スクイーズ バイオテクノロジーズ カンパニー 複合体の細胞内送達
WO2017173373A1 (en) 2016-03-31 2017-10-05 Massachusetts Institute Of Technology Flow-through microfluidic methods and devices featuring membrane-perturbing surface interactions for intracellular delivery
IL262677B2 (en) 2016-05-03 2025-08-01 Sqz Biotechnologies Co Intracellular delivery of biomolecules to suppress an immune response or to induce tolerance to an antigen in an individual
EP3452062A1 (en) 2016-05-03 2019-03-13 SQZ Biotechnologies Company Intracellular delivery of biomolecules to induce tolerance
CN106378213B (zh) * 2016-05-05 2018-08-17 海南大学 基于介电泳的可变形微颗粒分离芯片
WO2018039084A1 (en) 2016-08-20 2018-03-01 The Regents Of The University Of California High-throughput system and method for the temporary permeablization of cells
WO2018089497A1 (en) * 2016-11-08 2018-05-17 Georgia Tech Research Corporation Methods for convectively-driven intracellular delivery
TW201839136A (zh) 2017-02-06 2018-11-01 瑞士商諾華公司 治療血色素異常症之組合物及方法
US11383241B2 (en) * 2017-10-11 2022-07-12 The Regents Of The University Of California Mechano-node pore sensing
WO2019089034A1 (en) * 2017-11-02 2019-05-09 The Methodist Hospital Crispr-cas9 delivery to hard-to-transfect cells via membrane deformation
EP3720878A1 (en) 2017-12-05 2020-10-14 SQZ Biotechnologies Company Intracellular delivery of biomolecules to modulate antibody production
WO2019126146A1 (en) 2017-12-19 2019-06-27 Xcell Biosciences, Inc. Methods of modulating cell phenotype by way of regulating the gaseous environment
IL320767A (en) 2017-12-20 2025-07-01 Stemcell Technologies Canada Inc System for delivery of a payload into a cell
JP2019154314A (ja) * 2018-03-13 2019-09-19 国立大学法人九州大学 キャピラリーチップ及びこれを備える装置、並びに細胞への外来物質の導入方法
EP3556845A1 (en) 2018-04-20 2019-10-23 Cellix Limited A method and device for transfecting cells
CN109351370B (zh) * 2018-11-21 2020-05-05 晶准生物医学（深圳）有限公司 微流控芯片和细胞筛选方法
AU2020212601A1 (en) 2019-01-25 2021-09-09 Sqz Biotechnologies Company Anucleate cell-derived vaccines
EP3931309A4 (en) 2019-02-28 2022-12-21 SQZ Biotechnologies Company ADMINISTRATION OF BIOMOLECULES TO PBMC TO MODIFY AN IMMUNE RESPONSE
DE102019108155B3 (de) 2019-03-29 2020-06-04 Leibniz-Institut Für Photonische Technologien E.V. Mikrotropfenrückhalteanordnung
CN113840906B (zh) 2019-04-08 2024-09-06 Sqz生物技术公司 用于在将有效载荷递送至细胞中的系统中使用的试剂盒
WO2020207456A1 (en) * 2019-04-11 2020-10-15 The Chinese University Of Hong Kong Systems and methods for controlled membrane disruption
WO2020232418A1 (en) * 2019-05-15 2020-11-19 Cellfe, Inc. Intracellular delivery
WO2020252215A1 (en) 2019-06-12 2020-12-17 Cellfe, Inc. Methods and systems for cell labeling and imaging
EP4077676A1 (en) 2019-12-18 2022-10-26 Novartis AG Compositions and methods for the treatment of hemoglobinopathies
CN111413257B (zh) * 2020-01-21 2021-05-14 中国科学院电子学研究所 细胞核电学性能检测装置及方法
CA3177288A1 (en) 2020-05-11 2021-11-18 F.Hoffmann-La Roche Ag Combination therapy with modified pbmcs and an immunoconjugate
EP4158039A4 (en) * 2020-05-26 2024-11-13 Transcytos, LLC DEVICES AND METHODS FOR TRANSFECTION
KR20230058388A (ko) 2020-07-29 2023-05-03 에스큐지 바이오테크놀로지스 컴퍼니 무핵 세포를 사용해 돌연변이체 ras에 대한 면역 반응을 자극하는 방법
US20250255961A1 (en) 2020-07-29 2025-08-14 Stemcell Technologies Canada Inc. Methods to stimulate immune responses to mutant ras using nucleated cells
EP4232554A1 (en) 2020-11-18 2023-08-30 Cellfe, Inc. Methods and systems for mechanoporation-based payload delivery into biological cells
US12227729B2 (en) 2020-12-24 2025-02-18 Cellfe, Inc. Methods and systems for high-throughput cell processing
CA3203356A1 (en) 2020-12-29 2022-07-07 Oliver Rosen Methods for treating cancers with activating antigen carriers
WO2022147428A1 (en) 2020-12-29 2022-07-07 Sqz Biotechnologies Company Microfluidic chip having increased throughput for use in a system for delivery of a payload into a cell
JP2024503277A (ja) 2020-12-29 2024-01-25 スクイーズ バイオテクノロジーズ カンパニー 活性化抗原担体の製剤
US12150982B2 (en) 2020-12-29 2024-11-26 Stemcell Technologies Canada Inc. Formulations of PBMCs
JP2024502791A (ja) 2020-12-29 2024-01-23 スクイーズ バイオテクノロジーズ カンパニー 細胞膜を摂動させるための並列化された狭窄部を有する高スループットマイクロ流体チップ
WO2022147026A1 (en) 2020-12-29 2022-07-07 Sqz Biotechnologies Company METHODS FOR TREATING CANCERS WITH MODIFIED PBMCs
EP4359527A2 (en) 2021-06-23 2024-05-01 Novartis AG Compositions and methods for the treatment of hemoglobinopathies
EP4377447A1 (en) 2021-07-29 2024-06-05 SQZ Biotechnologies Company Methods to generate enhanced tumor infiltrating lymphocytes through microfluidic delivery
JP2024545582A (ja) 2021-11-11 2024-12-10 ステムセル テクノロジーズ カナダ インコーポレイテッド マイクロ流体送達による増強された腫瘍浸潤リンパ球を作製する方法
JP2025517524A (ja) 2022-05-27 2025-06-05 ステムセル テクノロジーズ カナダ インコーポレイテッド 細胞内にペイロードを送達するためのシステムで使用するカートリッジ及びデバイス
CA3262748A1 (en) 2022-07-28 2024-02-01 Stemcell Technologies Canada Inc. IMPROVED ANTIGEN-PRESENTING CELL FORMULATIONS
WO2024026492A1 (en) 2022-07-28 2024-02-01 Sqz Biotechnologies Company Methods for treating cancer with enhanced antigen presenting cells

Family Cites Families (123)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2502621C3 (de) 1975-01-23 1978-09-14 Kernforschungsanlage Juelich Gmbh, 5170 Juelich Messung elastischer und dielektrischer Eigenschaften der Membran lebender Zellen
US4376634A (en) 1980-05-30 1983-03-15 Mallinckrodt, Inc. Assay kit having syringe, dilution device and reagents within sealed container
US4478824A (en) 1983-08-08 1984-10-23 Franco Robert S Method for altering red blood cell function and survival
FR2569477B1 (fr) 1984-08-24 1987-01-02 Descartes Universite Rene Appareil et procede pour la determination de la deformabilite des globules rouges du sang
JP2720161B2 (ja) 1988-02-01 1998-02-25 株式会社アドバンス 細胞変形能測定装置
JP2685544B2 (ja) 1988-11-11 1997-12-03 株式会社日立製作所 血液フィルタおよび血液検査方法並びに血液検査装置
JP2532707B2 (ja) 1990-03-08 1996-09-11 佑二 菊池 血液回路及びこれを用いた血液測定装置及び血液測定方法
DK0528859T3 (da) 1990-04-24 1998-09-14 Flustat Pty Ltd Oral vaccine, som omfatter antigen knyttet til overfladen af røde blodlegemer
US5658892A (en) 1993-01-15 1997-08-19 The General Hospital Corporation Compound delivery using high-pressure impulse transients
US6218166B1 (en) 1994-12-09 2001-04-17 John Wayne Cancer Institute Adjuvant incorporation into antigen carrying cells: compositions and methods
DK0814838T3 (da) 1995-03-08 2003-09-15 Scripps Research Inst Antigenpræsenterende system og aktivering af T-celler
US6051409A (en) 1995-09-25 2000-04-18 Novartis Finance Corporation Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells
US6133503A (en) 1995-10-31 2000-10-17 The Regents Of The University Of California Mammalian artificial chromosomes and methods of using same
DE69626853T2 (de) 1995-12-04 2004-02-19 Puget Sound Blood Center And Programm, Seattle Nicht-immunogene Blutplättchen und rote Blutzellen enthaltende Zubereitungen
JP2000516911A (ja) 1996-03-28 2000-12-19 ホワイトヘッド インスティテュート フォー バイオメディカル リサーチ オプソニン強化細胞、および抗原に対する免疫応答を調節する方法
US5885470A (en) 1997-04-14 1999-03-23 Caliper Technologies Corporation Controlled fluid transport in microfabricated polymeric substrates
EP0882448B1 (en) 1997-05-05 2005-01-12 DIDECO S.r.l. Method of encapsulating biologically active agents within erythrocytes and apparatus therefor
US5842787A (en) 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
SE9704076D0 (sv) 1997-11-06 1997-11-06 Holdingbolaget Vid Goeteborgs Method for permeabilisation of cell structures and use thereof
GB9816583D0 (en) 1998-07-31 1998-09-30 Univ Ulster Nucleic acid carrier
WO2000078920A1 (en) 1999-06-21 2000-12-28 The General Hospital Corporation Methods and devices for cell culturing and organ assist systems
JP2002325572A (ja) 2000-12-25 2002-11-12 Univ Osaka 外来物質の導入方法
AU2002252073A1 (en) 2001-02-22 2002-09-12 The Scepens Eye Research Institute, Inc. Tolerogenic antigen presenting cells and in treating immune-inflammatory conditions
WO2003020039A1 (en) 2001-08-28 2003-03-13 Rush-Presbyterian-St. Luke's Medical Center Immune tolerance to predetermined antigens
CA2468424A1 (en) 2001-11-27 2003-06-05 Cecilia Farre A method for combined parallel agent delivery and electroporation for cell structures and use thereof
US20030133922A1 (en) 2002-01-15 2003-07-17 Kasha John R. Oral tolerance using allogeneic platelets in ITP
EP2365325A1 (en) 2002-06-05 2011-09-14 Panasonic Corporation Device for measuring extracellular potential and method of manufacturing the device
GB0214528D0 (en) 2002-06-24 2002-08-07 Univ Aberdeen Materials and methods for induction of immune tolerance
EP1587908A4 (en) 2003-01-09 2008-02-20 Invitrogen Corp RELEASE AND ACTIVATION OF COMPLEX CELLS OF POLYPEPTIDES AND NUCLEIC ACIDS
US20060134067A1 (en) 2003-02-18 2006-06-22 Maxcyte, Inc. Loading of cells with antigens by electroporation
EP1780266A4 (en) 2004-06-17 2009-09-09 Ken Nakata CELL CULTIVATION PROCESS WITH BIOMECHANICAL LOAD TREATMENT AND ITS DEVICE
CA2574128C (en) 2004-07-27 2012-10-09 Dsm Ip Assets B.V. Process for making a carbon nanotubes / ultra-high molar mass polyethylene composite fibre
FR2873925B1 (fr) 2004-08-05 2006-10-13 Erytech Pharma Soc Par Actions Procede et dispositif de lyse-rescellement pour l'incorporation de principe actif notamment asparaginase ou inositol hexaphosphate, dans des erythrocytes
ES2350895T3 (es) 2004-08-19 2011-01-28 University College Cardiff Consultants Limited Preparación de células t gamma delta humanas presentadoras de antígeno y su uso en inmunoterapia.
US20060134772A1 (en) 2004-11-18 2006-06-22 The Regents Of The University Of California System for locating cells and for cellular analysis
WO2006095330A2 (en) 2005-03-10 2006-09-14 Yeda Research And Development Co. Ltd. Methods and immunogenic cell preparations for treating antigen-associated diseases
US7704743B2 (en) 2005-03-30 2010-04-27 Georgia Tech Research Corporation Electrosonic cell manipulation device and method of use thereof
KR100760309B1 (ko) 2005-07-06 2007-10-05 한국과학기술원 미소필터를 이용한 미소입자 변형성 분석기
US7993821B2 (en) 2005-08-11 2011-08-09 University Of Washington Methods and apparatus for the isolation and enrichment of circulating tumor cells
US9005974B2 (en) 2005-12-09 2015-04-14 Academish Medisch Centrum Bij De Universiteit Van Amsterdam Means and methods for influencing the stability of cells
WO2007097934A2 (en) 2006-02-17 2007-08-30 Elusys Therapeutics, Inc. Methods and compositions for using erythrocytes as carriers for delivery of drugs
US8293524B2 (en) 2006-03-31 2012-10-23 Fluxion Biosciences Inc. Methods and apparatus for the manipulation of particle suspensions and testing thereof
US20070249038A1 (en) 2006-04-21 2007-10-25 Andrea Adamo Microfluidic device for single cell targeted operations
EP2054499A2 (en) 2006-08-17 2009-05-06 Massachusetts Institute of Technology Method and apparatus for microfluidic injection
WO2008098094A1 (en) 2007-02-06 2008-08-14 Network Biosystems, Inc. Devices and methods for the performance of miniaturized in vitro assays
US20080311140A1 (en) 2007-05-29 2008-12-18 Baylor College Of Medicine Antigen specific immunosuppression by dendritic cell therapy
US8841135B2 (en) * 2007-06-20 2014-09-23 University Of Washington Biochip for high-throughput screening of circulating tumor cells
US8420891B2 (en) 2007-06-21 2013-04-16 National University Corporation Nagoya University Method for introducing foreign substance into cell having cell wall
FR2919804B1 (fr) 2007-08-08 2010-08-27 Erytech Pharma Composition et vaccin therapeutique anti-tumoral
GB0718160D0 (en) 2007-09-18 2007-10-24 Medical Res Council Methods
EP2057998A1 (en) 2007-10-31 2009-05-13 Universitätsklinikum Hamburg-Eppendorf Use of modified cells for the treatment of multiple sclerosis
KR100891487B1 (ko) 2007-11-01 2009-04-01 한국과학기술원 미세유체제어기술 기반으로 한 난모세포 또는 난자의 핵제거 시스템
CN101874201B (zh) 2007-11-28 2012-08-15 柯尼卡美能达精密光学株式会社 血液流动性测量装置及血液流动性测量方法
US20090280518A1 (en) 2008-05-12 2009-11-12 Massachusetts Institute Of Technology System for high throughput measurement of mechanical properties of cells
JP5137129B2 (ja) 2008-07-23 2013-02-06 国立大学法人山口大学 血液細胞の力学的特性測定装置
EP2321405A4 (en) 2008-08-08 2012-09-12 Agency Science Tech & Res Microfluidic continuous flow device
US8211656B2 (en) 2008-08-13 2012-07-03 The Invention Science Fund I, Llc Biological targeting compositions and methods of using the same
US20110091973A1 (en) 2008-12-09 2011-04-21 Glaser Larry F Modified and fusion enhanced erythrocytes, cells and uses thereof
US9157550B2 (en) 2009-01-05 2015-10-13 The Board Of Trustees Of The University Of Illinois Microfluidic systems and methods
EP2405959A4 (en) 2009-03-13 2013-10-16 Univ Tufts METHODS, DEVICES AND KITS FOR INTRODUCING GENETIC MATERIAL IN LIVING CELLS
US9017991B2 (en) 2009-03-13 2015-04-28 Tufts University Methods tip assemblies and kits for introducing material into cells
WO2010107399A1 (en) 2009-03-20 2010-09-23 Agency For Science, Technology And Research Devices for separating cells and methods of using them
JP4849278B2 (ja) 2009-03-27 2012-01-11 セイコーエプソン株式会社 細胞処理デバイス、細胞処理カートリッジおよび体液処理システム
WO2010129671A2 (en) 2009-05-08 2010-11-11 Chauncey Sayre Method of and apparatus for changing one type of cell into another type of cell
GB0909754D0 (en) 2009-06-05 2009-07-22 Magnani Mauro Drug delivery systems
EP2449135B1 (en) 2009-06-30 2016-01-06 Sangamo BioSciences, Inc. Rapid screening of biologically active nucleases and isolation of nuclease-modified cells
US20110300205A1 (en) 2009-07-06 2011-12-08 Novartis Ag Self replicating rna molecules and uses thereof
KR101125060B1 (ko) * 2009-07-22 2012-03-21 한국과학기술원 입자를 포획하는 미세유체 소자 및 이를 이용한 입자 포획 방법
US9364831B2 (en) 2009-08-08 2016-06-14 The Regents Of The University Of California Pulsed laser triggered high speed microfluidic switch and applications in fluorescent activated cell sorting
JP5944318B2 (ja) 2009-10-27 2016-07-05 エリテック・ファルマ 特異的免疫寛容を誘導するための組成物
CN102791865B (zh) 2009-12-21 2015-07-01 凯津公司 用于转染原生质体的改进技术
JP5624629B2 (ja) 2009-12-23 2014-11-12 サイトベラ，インコーポレイテッド 粒子を濾過するためのシステム及び方法
WO2011084882A2 (en) 2010-01-05 2011-07-14 Contrafect Corporation Methods and compositions for enhanced immunological therapy and targeting of gram-positive bacteria
US8844570B2 (en) 2010-02-01 2014-09-30 California Institute Of Technology Generating binary states using a microfluidic channel
JP5704590B2 (ja) 2010-02-05 2015-04-22 国立大学法人東京農工大学 サイズ選択マイクロキャビティアレイを用いた循環腫瘍細胞の検出
US9458489B2 (en) 2010-03-04 2016-10-04 Massachusetts Institute Of Technology Microfluidics sorter for cell detection and isolation
US20110287948A1 (en) 2010-03-22 2011-11-24 Massachusetts Institute Of Technology Measurement of material properties and related methods and compositions based on cytoadherence
JP6343147B2 (ja) 2010-08-30 2018-06-13 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 狭窄病変および血栓溶解療法のための剪断による制御放出
WO2015061458A1 (en) 2013-10-22 2015-04-30 Cellanyx Diagnostics, Inc. Systems, devices and methods for microfluidic culturing, manipulation and analysis of tissues and cells
CN103261218B (zh) 2010-11-25 2020-08-14 Imnate责任有限公司 免疫原性肽在预防和/或治疗疾病中的应用
EP2665540A1 (en) * 2011-01-19 2013-11-27 The University of British Columbia Apparatus and method for particle separation
CA2828239C (en) 2011-02-25 2020-10-06 Recombinetics, Inc. Genetically modified animals and methods for making the same
CN103502426B (zh) 2011-02-28 2018-03-13 哈佛大学校长及研究员协会 细胞培养系统
CA2836608C (en) 2011-05-27 2016-10-11 The University Of British Columbia Microfluidic cell trap and assay apparatus for high-throughput analysis
MX2013013243A (es) 2011-07-05 2014-05-30 Sotio As Medios y metodos para activar la inmunoterapia celular del cancer utilizando celulas cancerosas destruidas mediante presion hidrostatica y celulas dendriticas.
US20130065314A1 (en) 2011-08-25 2013-03-14 Phytocentric Biotech Inc. Algal transformation systems, compositions and methods
CN107058101B (zh) 2011-10-17 2021-06-01 麻省理工学院 细胞内传递
JP2015520194A (ja) 2012-06-07 2015-07-16 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 薬物標的指向化のためのナノ療法
US9255245B2 (en) 2012-07-03 2016-02-09 Agilent Technologies, Inc. Sample probes and methods for sampling intracellular material
SG11201503059XA (en) 2012-10-23 2015-06-29 Toolgen Inc Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof
CN102925337B (zh) 2012-11-08 2014-06-18 武汉友芝友生物制药有限公司 一种微流体细胞捕获芯片及其制备方法
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
GB201300052D0 (en) 2013-01-03 2013-02-20 Transimmune Ag Method for obtaining immuno-suppressive dendritic cells
GB201300049D0 (en) 2013-01-03 2013-02-20 Transimmune Ag Method for obtaining immuno-stimulatory dendritic cells
WO2014120956A1 (en) 2013-01-30 2014-08-07 Asta Fluidic Technologies, Inc. Microfluidic-based fetal red blood cell detection
US9725709B2 (en) 2013-03-12 2017-08-08 OpenCell Technologies, Inc. Intracellular delivery and transfection methods and devices
CN105452471A (zh) 2013-03-15 2016-03-30 加利福尼亚大学董事会 使用光热平台向活细胞中的高通量运输物递送
KR20140115560A (ko) 2013-03-21 2014-10-01 인하대학교 산학협력단 기계적 스트레스를 이용한 단세포 생물체 개질 방법 및 이에 사용되는 장치
WO2014165707A2 (en) 2013-04-03 2014-10-09 Memorial Sloan-Kettering Cancer Center Effective generation of tumor-targeted t-cells derived from pluripotent stem cells
KR101508974B1 (ko) * 2013-05-14 2015-04-07 한국과학기술연구원 종양 세포의 검출장치 및 검출방법
KR102386122B1 (ko) 2013-08-16 2022-04-14 메사추세츠 인스티튜트 오브 테크놀로지 세포로의 선택적 물질 전달
WO2015103331A1 (en) 2013-12-31 2015-07-09 Canon U.S. Life Sciences, Inc. Methods and systems for continuous flow cell lysis in a microfluidic device
RU2736495C2 (ru) 2014-04-01 2020-11-17 Рубиус Терапьютикс, Инк. Способ и композиции для иммуномодуляции
EP3800248A3 (en) 2014-04-18 2021-08-04 Editas Medicine, Inc. Crispr-cas-related methods, compositions and components for cancer immunotherapy
EP3164482B1 (en) 2014-07-03 2024-05-22 Massachusetts Institute of Technology Apparatus and method for optimization of cell electroporation
CA2964392A1 (en) 2014-10-31 2016-05-06 Massachussetts Institute Of Technology Delivery of biomolecules to immune cells
CA2964138C (en) 2014-11-14 2023-11-14 Massachusetts Institute Of Technology Disruption and field enabled delivery of compounds and compositions into cells
US9744533B2 (en) 2014-12-10 2017-08-29 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
HK1247241A1 (zh) 2015-01-07 2018-09-21 英迪公司 一种用於机械和水动力微流体转染的方法以及用於其的设备
AU2016206870B2 (en) 2015-01-12 2022-02-17 Massachusetts Institute Of Technology Gene editing through microfluidic delivery
US20180135012A1 (en) 2015-05-13 2018-05-17 Rubius Therapeutics, Inc. Membrane-receiver complex therapeutics
GB201513278D0 (en) 2015-07-03 2015-09-09 Transimmune Ag And Yale University Device and method for obtaining immunostimulatory antigen-presenting cells
CA2988996A1 (en) 2015-07-09 2017-01-12 Massachusetts Institute Of Technology Delivery of materials to anucleate cells
EP3344575B1 (en) 2015-09-04 2020-04-15 SQZ Biotechnologies Company Intracellular delivery of biomolecules to cells comprising a cell wall
ES2930017T3 (es) 2015-09-04 2022-12-05 Sqz Biotechnologies Co Suministro intracelular de biomoléculas mediado por una superficie con poros
EP3393482A4 (en) 2015-12-22 2019-08-21 Sangui Bio Pty. Ltd THERAPEUTIC PROCEDURES USING ERYTHROCYTES
RS62939B1 (sr) 2016-01-11 2022-03-31 Rubius Therapeutics Inc Kompozicije i metode u vezi sa multimodalnim terapijskim ćelijskim sistemima u indikacijama kancera
JP7033535B2 (ja) 2016-01-12 2022-03-10 スクイーズ バイオテクノロジーズ カンパニー 複合体の細胞内送達
EP3452062A1 (en) 2016-05-03 2019-03-13 SQZ Biotechnologies Company Intracellular delivery of biomolecules to induce tolerance
IL262677B2 (en) 2016-05-03 2025-08-01 Sqz Biotechnologies Co Intracellular delivery of biomolecules to suppress an immune response or to induce tolerance to an antigen in an individual
US20170326213A1 (en) 2016-05-16 2017-11-16 Augusta University Research Institute, Inc. Protein-Coupled Red Blood Cell Compositions and Methods of Their Use
CN106244543A (zh) 2016-07-29 2016-12-21 北京时合生物科技有限公司 一种pbmc体外诱导分化成为树突状细胞的方法
WO2018089497A1 (en) 2016-11-08 2018-05-17 Georgia Tech Research Corporation Methods for convectively-driven intracellular delivery

Also Published As

Publication number Publication date
IL244113A0 (en) 2016-04-21
KR102243597B1 (ko) 2021-04-26
KR20210116682A (ko) 2021-09-27
SG10201801188XA (en) 2018-03-28
WO2015023982A1 (en) 2015-02-19
KR20160061332A (ko) 2016-05-31
US10870112B2 (en) 2020-12-22
US11806714B2 (en) 2023-11-07
KR20210043736A (ko) 2021-04-21
IL244113B (en) 2022-05-01
EP3033184A4 (en) 2017-03-29
CN105848793A (zh) 2016-08-10
MX2016002058A (es) 2016-08-17
EP3033184A1 (en) 2016-06-22
MX2021005777A (es) 2021-07-02
KR20220049050A (ko) 2022-04-20
ES2865107T3 (es) 2021-10-15
US20210170411A1 (en) 2021-06-10
SG11201601927SA (en) 2016-04-28
MX382654B (es) 2025-03-13
JP2016527907A (ja) 2016-09-15
CA2921579A1 (en) 2015-02-19
KR102386122B1 (ko) 2022-04-14
US10124336B2 (en) 2018-11-13
KR102530914B1 (ko) 2023-05-11
EP3033184B1 (en) 2021-03-31
US20160193605A1 (en) 2016-07-07
AU2019204480A1 (en) 2019-07-11
JP6502940B2 (ja) 2019-04-17
EP3848695A1 (en) 2021-07-14
CA2921579C (en) 2021-09-21
US20190030536A1 (en) 2019-01-31
AU2014306423B2 (en) 2019-04-18
AU2014306423A1 (en) 2016-03-17
KR102304167B1 (ko) 2021-09-24
CN105848793B (zh) 2019-03-05

Similar Documents

Publication Publication Date Title
PL3033184T3 (pl) Selektywne dostarczanie materiału do komórek
IL245573B (en) Cell
DK3461491T3 (da) Forbedret adoptiv celleterapi
LT3091141T (lt) Lengvesnės grindų plokštės iš mažiau medžiagų
EP2955772A4 (en) BATTERY PACK
ME03557B (me) Privremena zaštiтa normalnih ćelija током hemoterapije
ES1078818Y (es) Cápsula para preparar infusiones
DK3351620T3 (da) Metabolsk optimeret cellekultur
FR3003076B1 (fr) Supercondensateur electrochimique
EP2930778A4 (en) Electrode assembly
EP2911230A4 (en) CELL
PL3030519T3 (pl) Trójpierścieniowe związki benzoksaborolu i ich zastosowania
EP3066193C0 (en) CELL CULTURE PROCESS
PL3007261T3 (pl) Nowy akumulator
EP3057155A4 (en) BATTERY PACK
EP2866277A4 (en) BATTERY PACK
EP3076454A4 (en) BATTERY PACK
PL2900676T3 (pl) Ulepszony sposób acylowania majtansynolu
PL3066228T3 (pl) Sposób otrzymywania materiału fotokatalitycznego
EP2982171A4 (en) BACKGROUND SEARCH OF SMALL CELLS
EP3067936A4 (en) SOLAR CELL
FI20136038L (fi) Uudet materiaalit
EP3062822C0 (en) CELL TRANSPORT
EP3067940A4 (en) SOLAR CELL
FR3015112B1 (fr) Cellule photovoltaique