PT1609853E - Processo para controlar a sialilação de proteínas produzidas por cultura de células de mamífero - Google Patents

Processo para controlar a sialilação de proteínas produzidas por cultura de células de mamífero Download PDF

Info

Publication number
PT1609853E
PT1609853E PT05017434T PT05017434T PT1609853E PT 1609853 E PT1609853 E PT 1609853E PT 05017434 T PT05017434 T PT 05017434T PT 05017434 T PT05017434 T PT 05017434T PT 1609853 E PT1609853 E PT 1609853E
Authority
PT
Portugal
Prior art keywords
cell culture
mammalian cell
proteins produced
sialylation
controlling
Prior art date
Application number
PT05017434T
Other languages
English (en)
Portuguese (pt)
Inventor
Tina Etcheverry
Werner Lesslauer
Thomas Ryll
Thomas Schreitmueller
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23863440&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PT1609853(E) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of PT1609853E publication Critical patent/PT1609853E/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7151—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for tumor necrosis factor [TNF], for lymphotoxin [LT]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Rheumatology (AREA)
  • Pain & Pain Management (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
PT05017434T 1995-06-06 1996-06-06 Processo para controlar a sialilação de proteínas produzidas por cultura de células de mamífero PT1609853E (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/469,348 US5705364A (en) 1995-06-06 1995-06-06 Mammalian cell culture process

Publications (1)

Publication Number Publication Date
PT1609853E true PT1609853E (pt) 2009-06-09

Family

ID=23863440

Family Applications (1)

Application Number Title Priority Date Filing Date
PT05017434T PT1609853E (pt) 1995-06-06 1996-06-06 Processo para controlar a sialilação de proteínas produzidas por cultura de células de mamífero

Country Status (35)

Country Link
US (1) US5705364A (enExample)
EP (2) EP0832189B2 (enExample)
JP (2) JPH11507523A (enExample)
KR (1) KR100496356B1 (enExample)
CN (1) CN1166772C (enExample)
AR (2) AR004940A1 (enExample)
AT (2) ATE425245T2 (enExample)
AU (1) AU717847B2 (enExample)
BG (1) BG63213B1 (enExample)
BR (1) BR9609150A (enExample)
CA (1) CA2220684C (enExample)
CZ (1) CZ293719B6 (enExample)
DE (2) DE69635076T3 (enExample)
DK (2) DK0832189T4 (enExample)
EA (1) EA001215B1 (enExample)
ES (2) ES2324046T5 (enExample)
GE (1) GEP20012519B (enExample)
HU (1) HU226420B1 (enExample)
IL (1) IL122398A (enExample)
IS (1) IS2614B (enExample)
MY (1) MY113496A (enExample)
NO (1) NO322276B1 (enExample)
NZ (1) NZ310202A (enExample)
OA (1) OA10753A (enExample)
PL (1) PL185484B1 (enExample)
PT (1) PT1609853E (enExample)
RO (1) RO120267B1 (enExample)
SA (1) SA96170351B1 (enExample)
SI (2) SI1609853T2 (enExample)
SK (1) SK282658B6 (enExample)
TR (1) TR199701543T1 (enExample)
TW (2) TW516962B (enExample)
UA (1) UA47428C2 (enExample)
WO (1) WO1996039488A1 (enExample)
ZA (1) ZA964776B (enExample)

Families Citing this family (116)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10399023I2 (de) 1989-09-12 2006-11-23 Ahp Mfg B V TFN-bindende Proteine
US6656466B1 (en) * 1995-06-06 2003-12-02 Genetech, Inc. Human tumor necrosis factor—immunoglobulin(TNFR1-IgG1) chimera composition
US5705364A (en) † 1995-06-06 1998-01-06 Genentech, Inc. Mammalian cell culture process
JP4306813B2 (ja) 1995-09-19 2009-08-05 アスビオファーマ株式会社 動物細胞の新規培養方法
MA24169A1 (fr) * 1996-05-08 1997-12-31 Hoffmann La Roche Traitement de l'asthme a l'aide de la tnfr-ig
WO1999028455A1 (de) * 1997-12-03 1999-06-10 Roche Diagnostics Gmbh Verfahren zur herstellung von polypeptiden mit geeigneter glykosilierung
US6140445A (en) * 1998-04-17 2000-10-31 Crompton Corporation Silane functional oligomer
US6528286B1 (en) * 1998-05-29 2003-03-04 Genentech, Inc. Mammalian cell culture process for producing glycoproteins
EP1092037B1 (en) * 1998-05-29 2005-12-28 Genentech, Inc. Cell culture process for producing glycoproteins
TR200504220T2 (tr) 1998-12-17 2007-04-24 Biogen Idec Ma Inc. Aktif limfotoksin-beta reseptör imunoglobülin şimeAktif limfotoksin-beta reseptör imunoglobülin şimerik proteinlerinin yüksek düzey ifadesi ve saflaştrik proteinlerinin yüksek düzey ifadesi ve saflaştırılması için bir yöntem.ırılması için bir yöntem.
GB9828624D0 (en) * 1998-12-23 1999-02-17 Glaxo Group Ltd Production of proteins
KR100475417B1 (ko) * 1998-12-30 2005-07-18 씨제이 주식회사 시알산 함량이 높은 재조합 당단백질의 제조방법
US7294481B1 (en) 1999-01-05 2007-11-13 Immunex Corporation Method for producing recombinant proteins
ATE348173T1 (de) 1999-04-26 2007-01-15 Genentech Inc Zellenzuchtverfahren für glycoproteine
US6261805B1 (en) * 1999-07-15 2001-07-17 Boyce Thompson Institute For Plant Research, Inc. Sialyiation of N-linked glycoproteins in the baculovirus expression vector system
KR100394474B1 (ko) * 1999-12-18 2003-08-09 동아제약 주식회사 동물세포에서 재조합 당단백질의 연속식 대량생산 방법
ATE313554T1 (de) * 2000-05-16 2006-01-15 Lipoxen Technologies Ltd Derivatisierung von proteinen in wässrigem lösungsmittel
AU2001271021A1 (en) * 2000-07-05 2002-01-14 Japan As Represented By Secretary Of Osaka University Process for producing glycoprotein
US20030040095A1 (en) * 2001-03-16 2003-02-27 Achille Arini Method for the production of pharmaceutically active recombinant proteins
US20030087372A1 (en) * 2001-06-13 2003-05-08 Genentech, Inc. Methods of culturing animal cells and polypeptide production in animal cells
EP1497444B1 (en) * 2002-03-27 2015-11-04 Immunex Corporation Methods for increasing polypeptide production
US20030190710A1 (en) * 2002-03-28 2003-10-09 Devries Ruth L. Control of glycoforms in IgG
US7067279B1 (en) 2002-08-23 2006-06-27 Immunex Corporation Cell culture performance with betaine
US6974681B1 (en) 2002-08-23 2005-12-13 Immunex Corporation Cell culture performance with vanadate
US6924124B1 (en) * 2002-08-23 2005-08-02 Immunex Corporation Feeding strategies for cell culture
US7285269B2 (en) * 2002-12-02 2007-10-23 Amgen Fremont, Inc. Antibodies directed to tumor necrosis factor
PL377731A1 (pl) * 2002-12-23 2006-02-06 Bristol-Myers Squibb Company Sposoby hodowli komórek ssaczych do wytwarzania białka
PL377603A1 (pl) * 2002-12-23 2006-02-06 Bristol-Myers Squibb Company Poprawa jakości produktu w hodowlach komórek ssaczych do wytwarzania białka
MXPA05011486A (es) * 2003-04-25 2006-04-18 Immunex Corp Inductores de expresion de proteina recombinante.
SG147468A1 (en) 2003-10-24 2008-11-28 Selexis Sa High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences
AU2004309114A1 (en) * 2003-12-23 2005-07-14 Laboratoires Serono Sa Process for the production of tumor necrosis factor-binding proteins
EP1709185A1 (en) * 2003-12-31 2006-10-11 Samyang Genex Corporation Method for mass production of secondary metabolites in plant cell culture by treatment of an alkanoic acid or salt thereof
CN1926242B (zh) * 2004-02-13 2013-01-16 格莱克托普有限公司 高活性糖蛋白加工条件及其有效生产方法
US7294484B2 (en) * 2004-08-27 2007-11-13 Wyeth Research Ireland Limited Production of polypeptides
TWI364458B (en) * 2004-08-27 2012-05-21 Wyeth Res Ireland Ltd Production of tnfr-lg
TWI374935B (en) * 2004-08-27 2012-10-21 Pfizer Ireland Pharmaceuticals Production of α-abeta
AU2006247039B2 (en) * 2005-05-19 2011-03-03 Amgen Inc. Compositions and methods for increasing the stability of antibodies
CA2610315A1 (en) * 2005-06-03 2006-12-07 Biovitrum Ab (Publ) Process for cultivating animal cells comprising the feeding of plant-derived peptones
KR100670105B1 (ko) 2005-06-29 2007-01-17 주식회사 셀트리온 콩가수분해물의 저분자량 분획을 이용하여 시알산 함량이증가된 에리스로포이에틴을 생산하는 방법 및 그에 의하여생산된 시알산 함량이 증가된 에리스로포이에틴
WO2008057634A2 (en) * 2006-10-26 2008-05-15 The Rockefeller University Polypeptides with enhanced anti-inflammatory and decreased cytotoxic properties and relating methods
US8470318B2 (en) 2005-11-07 2013-06-25 The Rockefeller University Polypeptides with enhanced anti-inflammatory and decreased cytotoxic properties and relating methods
TWI423986B (zh) 2005-12-20 2014-01-21 必治妥美雅史谷比公司 組合物及製造組合物之方法
AR058568A1 (es) 2005-12-20 2008-02-13 Bristol Myers Squibb Co Metodos para producir una composicion con moleculas ctla4-ig a partir de un medio de cultivo
US20070190057A1 (en) 2006-01-23 2007-08-16 Jian Wu Methods for modulating mannose content of recombinant proteins
JP5199886B2 (ja) * 2006-01-26 2013-05-15 レコファーマ アーベー ウイルスの接着を阻害するための組成物および方法
US10167332B2 (en) * 2006-04-05 2019-01-01 The Rockefeller University Polypeptides with enhanced anti-inflammatory and decreased cytotoxic properties and relating methods
PE20081194A1 (es) * 2006-07-13 2008-09-22 Wyeth Corp Produccion de glicoproteinas
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
MX345141B (es) * 2006-09-13 2017-01-18 Abbvie Inc * Mejoras de cultivos celulares.
US20080145893A1 (en) * 2006-09-17 2008-06-19 Excellegene Sa Method for producing a recombinant protein at high specific productivity, high batch yield and high volumetric yield by means of transient transfection
WO2008069244A1 (ja) * 2006-12-05 2008-06-12 Kyowa Hakko Kogyo Co., Ltd. 糖蛋白質組成物の製造方法
CN101675339B (zh) * 2007-04-16 2014-04-16 动量制药公司 涉及细胞表面糖基化作用的方法
TW200902708A (en) 2007-04-23 2009-01-16 Wyeth Corp Methods of protein production using anti-senescence compounds
US20090023186A1 (en) * 2007-07-22 2009-01-22 Excellgene Sa Use of valproic acid for enhancing production of recombinant proteins in mammalian cells
WO2009023562A2 (en) * 2007-08-09 2009-02-19 Wyeth Use of perfusion to enhance production of fed-batch cell culture in bioreactors
EP3760715B1 (en) * 2008-03-06 2021-08-04 Halozyme, Inc. Large-scale production of soluble hyaluronidase
TWI395593B (zh) 2008-03-06 2013-05-11 Halozyme Inc 可活化的基質降解酵素之活體內暫時性控制
CA2720610C (en) * 2008-04-07 2016-08-23 Bayer Healthcare Llc Methods of recombinant production of glycoproteins
MX2010011598A (es) 2008-04-22 2010-12-15 Univ Rockefeller Metodos para identificar compuestos anti-inflamatorios.
EP2326720B1 (en) 2008-09-15 2018-03-21 F. Hoffmann-La Roche AG Compositions and methods for regulating cell osmolarity
TWI610936B (zh) * 2008-10-20 2018-01-11 艾伯維有限公司 使用蛋白質ａ親和性層析進行抗體之分離及純化
CN104974251A (zh) 2008-10-20 2015-10-14 Abbvie公司 在抗体纯化过程中的病毒灭活
EA022752B1 (ru) * 2008-12-09 2016-02-29 Галозим, Инк. Длинные растворимые полипептиды рн20 и их использование
CA2754461A1 (en) * 2009-03-06 2010-09-10 Halozyme, Inc. Temperature sensitive mutants of matrix metalloprotease 1 and uses thereof
US9540426B2 (en) 2009-10-06 2017-01-10 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production
JP5711751B2 (ja) 2009-10-26 2015-05-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ グリコシル化された免疫グロブリンの調製方法
EP3255153A1 (en) 2009-11-17 2017-12-13 E. R. Squibb & Sons, L.L.C. Methods for enhanced protein production
RU2012127383A (ru) * 2009-12-02 2014-01-10 Акселерон Фарма Инк. Композиции и способы для увеличения времени полужизни fc-слитых белков в сыворотке
HUE037943T2 (hu) * 2010-02-24 2018-09-28 Chiesi Farm Spa Eljárás rekombináns lizoszomális alfa-mannozidáz elõállítására és tisztítására
WO2012017925A1 (ja) 2010-08-02 2012-02-09 協和発酵キリン株式会社 物質の製造方法
WO2012122625A1 (en) 2011-03-14 2012-09-20 National Research Council Of Canada Method of viral production in cells
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
EP2702164B8 (en) 2011-04-29 2016-03-16 Biocon Research Limited A method for reducing heterogeneity of antibodies and a process of producing the antibodies thereof
WO2012170938A1 (en) 2011-06-08 2012-12-13 Acceleron Pharma Inc. Compositions and methods for increasing serum half-life
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
US9334319B2 (en) 2012-04-20 2016-05-10 Abbvie Inc. Low acidic species compositions
US9181572B2 (en) 2012-04-20 2015-11-10 Abbvie, Inc. Methods to modulate lysine variant distribution
WO2013176754A1 (en) 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
CN102839157A (zh) * 2012-07-12 2012-12-26 扬州大学 稳定共表达hST3GalIV和β1-4GalT1的MDCK细胞系及构建方法和应用
BR112015004467A2 (pt) 2012-09-02 2017-03-21 Abbvie Inc método para controlar a heterogeneidade de proteínas
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
NO2760138T3 (enExample) 2012-10-01 2018-08-04
US20140106405A1 (en) * 2012-10-15 2014-04-17 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production
CN104822826B (zh) 2012-10-15 2019-04-30 百时美施贵宝公司 用于蛋白产生的哺乳动物细胞培养过程
EP2830651A4 (en) 2013-03-12 2015-09-02 Abbvie Inc HUMAN ANTIBODIES THAT BIND TNF-ALPHA AND PREPARATION METHODS
US9217168B2 (en) 2013-03-14 2015-12-22 Momenta Pharmaceuticals, Inc. Methods of cell culture
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
US9499614B2 (en) 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
AR095196A1 (es) 2013-03-15 2015-09-30 Regeneron Pharma Medio de cultivo celular libre de suero
MX363403B (es) 2013-07-04 2019-03-22 Hoffmann La Roche Inmumoensayo con interferencia suprimida para la deteccion de anticuerpos anti-farmacos en muestras de suero.
MX2016000123A (es) 2013-07-06 2016-07-14 Cadila Healthcare Ltd Procedimiento mejorado para la produccion de anticuerpos monoclonales.
WO2015051293A2 (en) 2013-10-04 2015-04-09 Abbvie, Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
SI3110961T1 (sl) * 2014-02-27 2020-03-31 F. Hoffmann-La Roche Ag Modulacija rasti celic in glikozilacije pri rekombinantni proizvodnji glikoproteina
JP6652334B2 (ja) 2014-05-31 2020-02-19 Ｊｃｒファーマ株式会社 ウリジンとｎ−アセチル−ｄ−マンノサミンとを含有する培地
BR112016028538A2 (pt) * 2014-06-03 2017-08-22 Lupin Ltd processo de produção de proteína glicosilada, processos de produção de proteína de fusão e anticorpo monoclonal e fragmento do mesmo, processo de produção de proteína de fusão tnfr-fc, proteína de fusão ou anticorpo monoclonal e fragmento do mesmo
US20160130324A1 (en) 2014-10-31 2016-05-12 Shire Human Genetic Therapies, Inc. C1 Inhibitor Fusion Proteins and Uses Thereof
KR102007930B1 (ko) 2014-12-31 2019-08-06 주식회사 엘지화학 재조합 당단백질의 글리코실화 조절 방법
TWI797060B (zh) 2015-08-04 2023-04-01 美商再生元醫藥公司 補充牛磺酸之細胞培養基及用法
CN108463243B (zh) 2015-11-19 2022-06-14 夏尔人类遗传性治疗公司 重组人c1酯酶抑制剂及其用途
CN108882740A (zh) * 2015-12-29 2018-11-23 N·V·努特里奇亚 含有不可消化寡糖的发酵配方物
CN111406112A (zh) * 2017-11-30 2020-07-10 豪夫迈·罗氏有限公司 用于培养哺乳动物细胞的工艺
US11634499B2 (en) 2018-11-13 2023-04-25 Janssen Biotech, Inc. Control of trace metals during production of anti-CD38 antibodies
US12297451B1 (en) 2019-10-25 2025-05-13 Regeneron Pharmaceuticals, Inc. Cell culture medium
SG11202110968VA (en) 2019-12-06 2021-10-28 Regeneron Pharma Anti-vegf protein compositions and methods for producing the same
CA3182893A1 (en) 2020-05-08 2021-11-11 Regeneron Pharmaceuticals, Inc. Vegf traps and mini-traps and methods for treating ocular disorders and cancer
JP2023542820A (ja) 2020-08-31 2023-10-12 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 細胞培養成績を改善し、アスパラギン配列バリアントを軽減するためのアスパラギン供給戦略
WO2022159432A1 (en) 2021-01-20 2022-07-28 Regeneron Pharmaceuticals, Inc. Methods of improving protein titer in cell culture
EP4413364A2 (en) 2021-10-07 2024-08-14 Regeneron Pharmaceuticals, Inc. Systems and methods of ph modeling and control
US20230110811A1 (en) 2021-10-07 2023-04-13 Regeneron Pharmaceuticals, Inc. pH METER CALIBRATION AND CORRECTION
CN119053625A (zh) 2022-03-02 2024-11-29 瑞泽恩制药公司 用于抗体生产的细胞培养方法
WO2025219232A1 (en) 2024-04-15 2025-10-23 Evonik Operations Gmbh Application of small peptides supplemented cell culture media for improved cell performance

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3926723A (en) 1974-08-08 1975-12-16 Massachusetts Inst Technology Method of controllably releasing glucose to a cell culture medium
JPS5938488B2 (ja) 1979-05-07 1984-09-17 株式会社東芝 空気調和機
CS251766B2 (en) * 1982-06-21 1987-08-13 Wellcome Found Method of interferon production
US4560655A (en) 1982-12-16 1985-12-24 Immunex Corporation Serum-free cell culture medium and process for making same
US4657866A (en) 1982-12-21 1987-04-14 Sudhir Kumar Serum-free, synthetic, completely chemically defined tissue culture media
US4767704A (en) 1983-10-07 1988-08-30 Columbia University In The City Of New York Protein-free culture medium
US4724206A (en) * 1984-02-13 1988-02-09 Damon Biotech, Inc. Protein production using hypertonic media
GB8516415D0 (en) 1985-06-28 1985-07-31 Celltech Ltd Culture of animal cells
JPH0639919B2 (ja) 1985-08-29 1994-05-25 トヨタ自動車株式会社 過給機付き内燃機関の異常判別装置
GB8606386D0 (en) * 1986-03-14 1986-04-23 Celltech Ltd Production of protein
US4927762A (en) 1986-04-01 1990-05-22 Cell Enterprises, Inc. Cell culture medium with antioxidant
WO1988001643A1 (en) 1986-08-29 1988-03-10 Endotronics, Inc. Method of culturing cells
JPH01257492A (ja) * 1987-03-05 1989-10-13 Green Cross Corp:The 異種蛋白質の生産増強方法
IL87737A (en) * 1987-09-11 1993-08-18 Genentech Inc Method for culturing polypeptide factor dependent vertebrate recombinant cells
JPH066054B2 (ja) 1987-10-15 1994-01-26 帝人株式会社 動物細胞の培養方法
CA1312030C (en) * 1987-11-18 1992-12-29 Brian Maiorella Method to increase antibody titer
US5151359A (en) * 1988-05-19 1992-09-29 Mitsui Toatsu Chemicals Incorporated Method for producing of human tissue type plasminogen activator
EP0435911B1 (en) 1988-09-23 1996-03-13 Cetus Oncology Corporation Cell culture medium for enhanced cell growth, culture longevity and product expression
KR0132666B1 (en) * 1989-03-14 1998-04-14 Hitachi Kk Method for controlling cultivation conditions for animal cells
JPH0396383A (ja) 1989-09-08 1991-04-22 Riso Kagaku Corp 画像形成装置
DE10399023I2 (de) 1989-09-12 2006-11-23 Ahp Mfg B V TFN-bindende Proteine
US5096816A (en) * 1990-06-05 1992-03-17 Cetus Corporation In vitro management of ammonia's effect on glycosylation of cell products through pH control
US5122469A (en) * 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
GB9022545D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Culture medium
GB2251249B (en) * 1990-12-28 1995-06-21 Mogam Biotech Res Inst High-density medium for animal cell culture
WO1993010260A1 (en) * 1991-11-21 1993-05-27 The Board Of Trustees Of The Leland Stanford Junior University Controlling degradation of glycoprotein oligosaccharides by extracellular glycosisases
US5447851B1 (en) * 1992-04-02 1999-07-06 Univ Texas System Board Of Dna encoding a chimeric polypeptide comprising the extracellular domain of tnf receptor fused to igg vectors and host cells
NZ256293A (en) * 1992-09-15 1997-06-24 Immunex Corp Method of treating tumour necrosis factor - mediated inflammation by use of tnf antagonist
JP3523293B2 (ja) † 1993-07-19 2004-04-26 住友製薬株式会社 インターフェロンの産生増強法
US5705364A (en) † 1995-06-06 1998-01-06 Genentech, Inc. Mammalian cell culture process
JP5515221B2 (ja) † 2008-01-28 2014-06-11 日油株式会社 ポリオキシエチレンソルビタン脂肪酸エステルの製造方法

Also Published As

Publication number Publication date
US5705364A (en) 1998-01-06
ES2324046T3 (es) 2009-07-29
ES2324046T5 (es) 2020-09-14
NZ310202A (en) 1999-10-28
DK0832189T4 (da) 2008-10-27
EP1609853B2 (en) 2020-01-22
HU226420B1 (en) 2008-12-29
CN1166772C (zh) 2004-09-15
BG102101A (en) 1998-07-31
CN1186514A (zh) 1998-07-01
SI1609853T2 (sl) 2020-07-31
WO1996039488A1 (en) 1996-12-12
CZ390997A3 (cs) 1998-06-17
SI0832189T1 (sl) 2006-02-28
AU717847B2 (en) 2000-04-06
TW426734B (en) 2001-03-21
SI0832189T2 (sl) 2008-12-31
MY113496A (en) 2002-03-30
SA96170351B1 (ar) 2006-07-04
DK1609853T3 (da) 2009-06-02
EP0832189B2 (en) 2008-07-09
MX9709452A (es) 1998-06-30
HUP9900920A2 (hu) 1999-07-28
EP1609853A1 (en) 2005-12-28
IL122398A (en) 2001-12-23
BG63213B1 (bg) 2001-06-29
HUP9900920A3 (en) 2001-06-28
AU6095296A (en) 1996-12-24
JP2007190024A (ja) 2007-08-02
EP0832189A1 (en) 1998-04-01
DE69635076T2 (de) 2006-06-01
KR19990022473A (ko) 1999-03-25
ZA964776B (en) 1997-12-08
RO120267B1 (ro) 2005-11-30
UA47428C2 (uk) 2002-07-15
DE69635076T3 (de) 2009-01-15
SK282658B6 (sk) 2002-11-06
TW516962B (en) 2003-01-11
IS2614B (is) 2010-04-15
ATE302266T1 (de) 2005-09-15
EA001215B1 (ru) 2000-12-25
IL122398A0 (en) 1998-06-15
ES2248812T3 (es) 2006-03-16
TR199701543T1 (xx) 1998-05-21
IS4626A (is) 1997-12-03
GEP20012519B (en) 2001-08-27
DK1609853T4 (da) 2020-04-06
EP1609853B1 (en) 2009-03-11
PL323737A1 (en) 1998-04-14
DK0832189T3 (da) 2005-11-21
EP0832189B1 (en) 2005-08-17
CA2220684C (en) 2002-11-12
NO975674D0 (no) 1997-12-05
KR100496356B1 (ko) 2005-11-25
ATE425245T2 (de) 2009-03-15
DE69635076D1 (de) 2005-09-22
CA2220684A1 (en) 1996-12-12
DE69637867D1 (de) 2009-04-23
BR9609150A (pt) 1999-02-23
HK1017903A1 (en) 1999-12-03
PL185484B1 (pl) 2003-05-30
NO322276B1 (no) 2006-09-04
AR011439A2 (es) 2000-08-16
NO975674L (no) 1998-02-06
HK1087151A1 (en) 2006-10-06
EA199700364A1 (ru) 1998-04-30
SK167097A3 (en) 1998-07-08
JPH11507523A (ja) 1999-07-06
SI1609853T1 (sl) 2009-08-31
ES2248812T5 (es) 2008-12-16
CZ293719B6 (cs) 2004-07-14
JP4348376B2 (ja) 2009-10-21
OA10753A (en) 2002-12-11
AR004940A1 (es) 1999-04-07

Similar Documents

Publication Publication Date Title
HUP9900920A3 (en) Process for controlling sialylation of proteins produced by mammalian cell culture
AU1032597A (en) Process for preparing recombinant proteins in E. coli by means of high cell density fermentation
HUT71197A (en) Process for the production of amino acids by fermentation
EP0691400A4 (en) METHOD FOR EMBEDDING ANIMAL CULTURE CELLS
EP0509841A3 (en) Co-expression system of protein disulfide isomerase gene and useful polypeptide gene and process for producing the polypeptide using its system
AU7566396A (en) Process for the preparation of peptides by way of streptavidin fusion proteins
AU1406497A (en) Mutational variants of mammalian ob gene proteins
AU4493893A (en) Culture vessel for cell cultures
AU7719596A (en) Adenovirus-mediated production of bioactive proteins by mammalian cells and animals
HUP9603029A2 (en) Process for producing nicotinic acid amid
AU6405196A (en) Novel system for multilayer growth of anchorage-dependent cells
AU1556088A (en) Continuous process for ethanol production by bacterial fermentation using ph control
ZA917561B (en) Media for culture of mammalian cells
AU1428892A (en) Method of lyophilization of mammalian sperm cells
EP0165613A3 (en) Process for controlling culture of recombinants
LTIP1543A (en) Process for enzymatic decomposition of recombinant proteins by means of iga-protease, fused protein, recombinant dna, cell
AU5552996A (en) Purified mammalian t cell antigen crtam
AU5276196A (en) Method for the continuous preparation of recombinant proteinsfrom cell cultures
US5807733A (en) Mammalian prostaglandin H synthase-2 fusion proteins
AU6510196A (en) Regulated autocrine growth of mammalian cells
AU4772496A (en) Method for regulation of cell cycle
AU4076895A (en) Process for the mass production of bovine growth hormone
HU903494D0 (en) Process for fermentation by means of solid phase biocatalysts
KR0184848B1 (en) Method for mass production of taxol by semi-continuous culture
HK1013846A (en) Tight control of gene expression in eucaryotic cells by tetractcline-responsive promoters