KR930001117B1 - Dna 재조합 기술에 의한 폴리펩티드 제조방법 - Google Patents

Dna 재조합 기술에 의한 폴리펩티드 제조방법 Download PDF

Info

Publication number
KR930001117B1
KR930001117B1 KR1019850008020A KR850008020A KR930001117B1 KR 930001117 B1 KR930001117 B1 KR 930001117B1 KR 1019850008020 A KR1019850008020 A KR 1019850008020A KR 850008020 A KR850008020 A KR 850008020A KR 930001117 B1 KR930001117 B1 KR 930001117B1
Authority
KR
South Korea
Prior art keywords
dna
gene
polypeptide
yeast
medium
Prior art date
Application number
KR1019850008020A
Other languages
English (en)
Korean (ko)
Other versions
KR870003197A (ko
Inventor
워어맥크 스트로우먼 데이빗드
프레드릭 브러스트 폴
브래들리 엘리스 스티븐
레이몬드 진저러스 토마스
밀러 하아폴 마이클
프리드리히 초프 유에르크
Original Assignee
휘립프스 피트로오리암 캄파니
제이 이이 휘립프스
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/666,391 external-priority patent/US4808537A/en
Application filed by 휘립프스 피트로오리암 캄파니, 제이 이이 휘립프스 filed Critical 휘립프스 피트로오리암 캄파니
Publication of KR870003197A publication Critical patent/KR870003197A/ko
Application granted granted Critical
Publication of KR930001117B1 publication Critical patent/KR930001117B1/ko

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
KR1019850008020A 1984-10-30 1985-10-29 Dna 재조합 기술에 의한 폴리펩티드 제조방법 KR930001117B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US666.391 1984-10-30
US06/666,391 US4808537A (en) 1984-10-30 1984-10-30 Methanol inducible genes obtained from pichia and methods of use
US06/780,102 US4855231A (en) 1984-10-30 1985-09-25 Regulatory region for heterologous gene expression in yeast
US780102 1985-09-25
US780.102 1985-09-25

Publications (2)

Publication Number Publication Date
KR870003197A KR870003197A (ko) 1987-04-15
KR930001117B1 true KR930001117B1 (ko) 1993-02-18

Family

ID=27099454

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019850008020A KR930001117B1 (ko) 1984-10-30 1985-10-29 Dna 재조합 기술에 의한 폴리펩티드 제조방법

Country Status (23)

Country Link
US (1) US4855231A (da)
EP (2) EP0483115A3 (da)
JP (5) JP2502508B2 (da)
KR (1) KR930001117B1 (da)
CN (1) CN1011243B (da)
AR (1) AR242988A1 (da)
AT (1) ATE83263T1 (da)
AU (1) AU572002B2 (da)
CA (1) CA1340733C (da)
DD (1) DD254211A5 (da)
DE (1) DE3586889T2 (da)
DK (1) DK496385A (da)
ES (1) ES8609458A1 (da)
FI (1) FI94427C (da)
GR (1) GR852611B (da)
HU (1) HU205627B (da)
IE (1) IE930804L (da)
IL (1) IL76765A (da)
NO (1) NO178076C (da)
NZ (1) NZ213842A (da)
PL (1) PL158965B1 (da)
PT (1) PT81399B (da)
YU (1) YU46695B (da)

Families Citing this family (115)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5741672A (en) * 1984-07-27 1998-04-21 Unilever Patent Holdings B.V. Expression and production of polypeptides using the promoters of the hansenula polymorpha MOX and DAS genes
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US5032516A (en) * 1985-10-25 1991-07-16 Phillips Petroleum Company Pichia pastoris alcohol oxidase II regulatory region
US4882279A (en) * 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
US4818700A (en) * 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
US4895800A (en) * 1985-11-26 1990-01-23 Phillips Petroleum Company Yeast production of hepatitis B surface antigen
IL81817A0 (en) * 1986-03-14 1987-10-20 Phillips Petroleum Co Methanol and glucose responsive yeast regulatory regions
ZA872534B (en) * 1986-05-08 1987-11-25 Phillips Petroleum Co Yeast production of streptokinase
IL82935A0 (en) * 1986-08-12 1987-12-20 Phillips Petroleum Co Secretion of heterologous proteins from yeast
US5002876A (en) * 1986-09-22 1991-03-26 Phillips Petroleum Company Yeast production of human tumor necrosis factor
ES2056799T3 (es) * 1987-07-17 1994-10-16 Rhein Biotech Ges Fur Biotechn Moleculas de adn codante para las regiones de control fmdh y gen estructural para una proteina que tiene una actividad de fmdh y su uso.
ATE248925T1 (de) * 1988-01-05 2003-09-15 Roche Diagnostics Gmbh Verfahren zur herstellung von proteinen oder proteinhaltigen genprodukten
FI891695A (fi) * 1988-04-11 1989-10-12 Phillips Petroleum Co Expression av laxtillvaexthormon i methyltrofisk jaest av slaektet pichia.
IL90021A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Process for the production of interferon
NZ228774A (en) * 1988-04-25 1991-05-28 Phillips Petroleum Co Hbv particles containing pres 2 proteins and recombinant processes for their production in yeast cells
IL89992A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human serum albumin in methylotrophic yeasts
IL89989A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human interleukin-2 in methylotrophic yeasts
IL89993A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of the hiv 24kda gag protein in methylotrophic yeasts
DE3851535T2 (de) * 1988-12-20 1995-02-02 Rhein Biotech Proz & Prod Gmbh Mutanter Stamm von einem methylotrophen Organismus und Verfahren zur Herstellung eines Proteins in methylotrophen Organismus.
AU5196290A (en) * 1989-02-13 1990-09-05 Salk Institute Biotechnology/Industrial Associates, Inc., The Production of superoxide dismutase in pichia pastoris yeast cells
GB8924883D0 (en) * 1989-11-03 1989-12-20 Shell Int Research Expression vector and polypeptide synthesis
US5264554A (en) * 1990-01-19 1993-11-23 The Blood Center Of Southeastern Wisconsin, Inc. Platelet cell adhesion molecule and variants thereof
US6440681B1 (en) 1990-04-03 2002-08-27 Merck & Co., Inc. Methods for identifying agonists and antagonists for human neuronal nicotinic acetylcholine receptors
US5369028A (en) * 1990-04-03 1994-11-29 The Salk Institute Biotechnology/Industrial Associates, Inc. DNA and mRNA encoding human neuronal nicotinic acetylcholine receptor compositions and cells transformed with same
US5258302A (en) * 1990-07-03 1993-11-02 The Salk Institute Biotechnology/Industrial Associates, Inc. DNA for expression of aprotinin in methylotrophic yeast cells
JPH06500470A (ja) * 1990-09-04 1994-01-20 メルク　エンド　カンパニー　インコーポレーテッド メチロトローフ酵母細胞におけるインスリン様成長因子の産生
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5268273A (en) * 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells
DE69232666T2 (de) * 1991-04-01 2003-03-20 Merck & Co., Inc. Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung
CA2058820C (en) * 1991-04-25 2003-07-15 Kotikanyad Sreekrishna Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells
US5330901A (en) * 1991-04-26 1994-07-19 Research Corporation Technologies, Inc. Expression of human serum albumin in Pichia pastoris
US5834262A (en) * 1992-01-06 1998-11-10 E. I. Du Pont De Nemours And Company Oxidation of glycolic acid to glyoxylic acid using a microbial cell transformant as catalyst
DK0558024T3 (da) * 1992-02-28 1996-11-18 Suntory Ltd Ny vektor med en ved hjælp af methanol og/eller glycerol inducerbar promotor
US5708141A (en) * 1992-05-11 1998-01-13 Corvas International, Inc. Neutrophil inhibitors
CZ225795A3 (en) * 1993-03-03 1996-02-14 Du Pont Enzymatically active glycolate oxidase, process for preparing thereof, transformed methylotropic yeast, nucleic acid sequence and plasmid
ATE279516T1 (de) 1993-03-08 2004-10-15 Merck & Co Inc Menschliche neuronale nikotin-acetylcholin- rezeptor-verbindungen und methoden ihres einsatzes
AU6415594A (en) * 1993-03-25 1994-10-11 Biosource Genetics Corporation (pichia pastoris) alcohol oxidase (zza1) and (zza2)
WO1994024284A1 (en) 1993-04-20 1994-10-27 The Salk Institute Biotechnology/Industrial Associates, Inc. Human n-methyl-d-aspartate receptor subunits, nucleic acids encoding same and uses therefor
US5521297A (en) 1993-06-04 1996-05-28 Salk Institute Biotechnology/Industrial Associates Nucleic acids encoding human metabotropic glutamate receptors
US5912122A (en) * 1993-06-04 1999-06-15 Sibia Neurosciences, Inc. Nucleic acids encoding and method for detecting nucleic acid encoding human metabotropic glutamate receptor subtype mGluR6
US6001581A (en) * 1993-06-04 1999-12-14 Sibia Neurosciences, Inc. Cation-based bioassay using human metabotropic glutamate receptors
US6638905B2 (en) * 1993-06-18 2003-10-28 The Salk Institute For Biological Studies Cloning and recombinant production of CFR receptor(s)
US6495343B1 (en) 1993-06-18 2002-12-17 The Salk Institute For Biological Studies Cloning and recombinant production of CRF receptor(s)
WO1995013299A1 (en) * 1993-11-08 1995-05-18 The Salk Institute Biotechnology/Industrial Associates, Inc. Human neuronal nicotinic acetylcholine receptor compositions and methods employing same
US5453161A (en) * 1993-11-12 1995-09-26 Enichem S.P.A. Polyamic acid to polyimide conversion by microwave heating
US5945275A (en) * 1994-10-18 1999-08-31 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5863894A (en) * 1995-06-05 1999-01-26 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5866542A (en) * 1994-10-18 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
EP0788546B9 (en) 1994-10-18 2007-06-13 Dendreon Corporation Nematode-extracted serine protease inhibitors and anticoagulant proteins
CA2202351A1 (en) * 1994-10-18 1996-04-25 George Phillip Vlasuk Nematode-extracted serine protease inhibitors and anticoagulant proteins
US5866543A (en) * 1995-06-05 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5872098A (en) * 1995-06-05 1999-02-16 Corvas International, Inc. Nematode-extracted anticoagulant protein
US6485967B1 (en) 1995-06-07 2002-11-26 Merck & Co., Inc. Human neuronal nicotinic acetylcholine receptor α6 and β3 nucleic acid
US6720140B1 (en) * 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6143557A (en) 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
WO1997017450A2 (en) * 1995-11-09 1997-05-15 Zymogenetics, Inc. Compositions and methods for producing heterologous polypeptides in pichia methanolica
MX9605082A (es) 1996-10-24 1998-04-30 Univ Autonoma De Nuevo Leon Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano.
EP0911416B1 (en) 1997-10-01 2006-05-17 DSM IP Assets B.V. Protein production process
CN100342008C (zh) * 1997-10-24 2007-10-10 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
WO2000001731A1 (fr) * 1998-07-06 2000-01-13 Tosoh Corporation Proteine fusionnee avec liaison directe du recepteur il-6 a il-6
EP1114148A4 (en) * 1998-08-28 2004-12-22 Invitrogen Corp SYSTEM FOR RAPID HANDLING OF NUCLEIC ACID SEQUENCES
US6780615B1 (en) 1998-12-31 2004-08-24 Genway Biotech Inc. Production of recombinant monellin using methylotrophic yeast expression system
US6720174B1 (en) 1999-01-28 2004-04-13 Novozymes A/S Phytases
CA2363924A1 (en) 1999-03-02 2000-09-08 Invitrogen Corporation Compositions and methods for use in recombinational cloning of nucleic acids
US7504253B2 (en) * 1999-06-11 2009-03-17 The Burnham Institute For Medical Research Nucleic acid encoding proteins involved in protein degradation, products and methods related thereof
US6440414B1 (en) * 1999-10-01 2002-08-27 Amgen Inc. Pharmaceutical compositions of fibrinolytic agent
US6261820B1 (en) * 1999-10-01 2001-07-17 Amgen Inc. Fibronolytically active polypeptide
EP2011874A1 (en) 1999-11-12 2009-01-07 Fibrogen, Inc. Recombinant gelatin in vaccines
AU785483B2 (en) 1999-12-10 2007-09-20 Invitrogen Corporation Use of multiple recombination sites with unique specificity in recombinational cloning
US7033776B2 (en) * 1999-12-17 2006-04-25 Amgen Inc. Method for treatment of indwelling catheter occlusion using fibrinolytic metalloproteinases
WO2001077351A1 (en) * 2000-04-07 2001-10-18 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Vectors and methods for dual protein expression in pichia pastoris and escherichia coli
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7449308B2 (en) * 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
DK1522590T3 (da) 2000-06-28 2009-12-21 Glycofi Inc Fremgangsmåde til fremstilling af modificerede glykoproteiner
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US7863020B2 (en) * 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
JP4092194B2 (ja) 2000-06-30 2008-05-28 フランダース インターユニバーシティ インスティテュート フォア バイオテクノロジー （ヴィーアイビー） ピキアパストリス（ＰｉｃｈｉａＰａｓｔｏｒｉｓ）におけるタンパク質糖鎖修飾
US7009045B2 (en) * 2000-07-14 2006-03-07 Archer-Daniels-Midland Company Transformation systems for flavinogenic yeast
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
MXPA03009059A (es) * 2001-04-05 2004-10-15 Univ Nebraska Secuencias de nucleotidos reguladoras de alcohol oxidasa 1 para expresion de genes heterologos en levadura.
WO2002095055A2 (en) * 2001-05-21 2002-11-28 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
US6645739B2 (en) 2001-07-26 2003-11-11 Phoenix Pharmacologies, Inc. Yeast expression systems, methods of producing polypeptides in yeast, and compositions relating to same
CA2482485A1 (en) * 2002-04-15 2003-10-30 Merck & Co., Inc. Matrix analysis of gene expression in cells (magec)
US7252933B2 (en) 2002-06-26 2007-08-07 Flanders Interuniversity Institute For Biotechnology Protein glycosylation modification in methylotrophic yeast
US20040204953A1 (en) * 2002-07-17 2004-10-14 Lincoln Muir Subscription based systems, methods and components for providing genomic and proteomic products and services
US7118901B2 (en) * 2002-12-18 2006-10-10 Roche Diagnostics Operations, Inc. Recombinant bovine pancreatic desoxyribonuclease I with high specific activity
ATE387494T1 (de) * 2002-12-20 2008-03-15 Hoffmann La Roche Hitzelabile desoxyribonuklease i-varianten
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
EP1460425A1 (en) * 2003-03-17 2004-09-22 Boehringer Mannheim Gmbh Deglycosylated enzymes for conjugates
CA2536635C (en) 2003-08-25 2015-05-12 Funzyme Biotechnologies Sa Novel fungal proteins and nucleic acids encoding same
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
DE602004027538D1 (de) 2003-12-01 2010-07-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
ES2337684T3 (es) * 2003-12-05 2010-04-28 F. Hoffmann-La Roche Ag Carboxipeptidasa b recombinante y su purificacion.
MY146082A (en) * 2005-09-14 2012-06-29 Sanofi Aventis Deutschland Cleavage of precursors of insulins by a variant of trypsin
AT504588B1 (de) * 2006-11-16 2008-08-15 Univ Graz Tech Nukleinsäure-promotor
US9200270B2 (en) * 2008-03-03 2015-12-01 Abbvie Inc. Methods for transforming yeast
KR20110101198A (ko) 2008-12-12 2011-09-15 이 아이 듀폰 디 네모아 앤드 캄파니 재생 가능 자원으로부터의 선형 다이카르복실산의 제조 방법
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
AU2010334383B2 (en) 2009-12-21 2014-10-02 Centre Hospitalier Universitaire Vaudois (Chuv) Synergic action of a prolyl protease and tripeptidyl proteases
MX368546B (es) 2011-05-20 2019-10-07 Alderbio Holdings Llc Producción en alta pureza de proteínas multi-subunitarias tales como anticuerpos en microbios transformados tal como pichia pastoris.
CN103890178A (zh) 2011-08-19 2014-06-25 奥尔德生物控股有限责任公司 转化微生物如巴斯德毕赤酵母中多亚基蛋白如抗体的高滴度和高纯度生成的多拷贝方案
KR101954389B1 (ko) 2011-10-07 2019-03-06 론자 리미티드 조절가능한 프로모터
US9150870B2 (en) 2013-03-15 2015-10-06 Lonza Ltd. Constitutive promoter
KR102202477B1 (ko) 2013-03-15 2021-01-13 룬드벡 시애틀 바이오파마슈티컬즈, 인크. 효모 및 기타 형질전환 세포에서 폴리펩티드의 고수율 발현을 위한 온도 전환
EP2970994B1 (en) 2013-03-15 2019-07-17 Lonza Ltd Constitutive promoter
EP3754012A1 (en) 2013-03-15 2020-12-23 Alder Biopharmaceuticals, Inc. Antibody purification and purity monitoring
CN105143243B (zh) 2013-03-21 2020-03-13 国家科学和工业研究组织 三螺旋蛋白的纯化
MX362515B (es) 2013-04-30 2019-01-22 Donald Danforth Plant Science Center Proteinas péptidos y métodos de uso de las plantas antifúngicas.
WO2015031950A1 (en) 2013-09-09 2015-03-12 Commonwealth Scientific And Industrial Research Organisation Modified bacterial collagen-like proteins
WO2016120764A1 (en) 2015-01-26 2016-08-04 Centre Hospitalier Universitaire Vaudois (Chuv) Aspergillus oryzae prolyl endopeptidases and use thereof in degradation of polypeptides

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK368882A (da) * 1981-08-25 1983-02-26 Alan John Kingsman Expessions vektorer
IE54592B1 (en) * 1982-03-08 1989-12-06 Genentech Inc Anumal interferons, processes involved in their production, compositions containing them, dna sequences coding therefor and espression vehicles containing such sequences and cells transformed thereby
EP0096910B1 (en) * 1982-05-19 1989-04-19 Unilever N.V. Yeast of the genus kluyveromyces modified for the expression of preprothaumatin or its various allelic and modified forms or their maturation forms
EP0118551A1 (en) * 1982-09-15 1984-09-19 Collaborative Research Inc. Production of interferon in yeast by use of invertase promoter
EP0423890A3 (en) * 1984-07-27 1991-07-03 Unilever Nv Use of oxidoreductases in bleaching and/or detergent compositions and their preparation by microorganisms engineered by recombinant dna technology
US4885242A (en) * 1984-10-30 1989-12-05 Phillips Petroleum Company Genes from pichia histidine pathway and uses thereof
US4837148A (en) * 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia

Also Published As

Publication number Publication date
CN1011243B (zh) 1991-01-16
FI94427C (fi) 1995-09-11
US4855231A (en) 1989-08-08
PT81399B (pt) 1987-11-11
AU4875285A (en) 1986-06-12
JPS61173781A (ja) 1986-08-05
AR242988A1 (es) 1993-06-30
PL256012A1 (en) 1986-09-23
DK496385A (da) 1986-05-01
DD254211A5 (de) 1988-02-17
NO178076C (no) 1996-01-17
NO178076B (no) 1995-10-09
PT81399A (en) 1985-11-01
CN85109718A (zh) 1986-08-20
JP2552807B2 (ja) 1996-11-13
EP0483115A3 (en) 1992-05-06
EP0183071A2 (en) 1986-06-04
DE3586889T2 (de) 1993-04-15
CA1340733C (en) 1999-09-14
GR852611B (da) 1986-03-04
EP0183071A3 (en) 1987-09-23
NZ213842A (en) 1991-11-26
YU46695B (sh) 1994-04-05
DK496385D0 (da) 1985-10-29
DE3586889D1 (de) 1993-01-21
JPH08173161A (ja) 1996-07-09
HU205627B (en) 1992-05-28
ATE83263T1 (de) 1992-12-15
EP0183071B1 (en) 1992-12-09
FI854142L (fi) 1986-05-01
IE930804L (en) 1986-04-30
EP0483115A2 (en) 1992-04-29
ES548307A0 (es) 1986-09-01
YU169585A (en) 1991-04-30
JPH08196275A (ja) 1996-08-06
IL76765A (en) 1991-06-30
KR870003197A (ko) 1987-04-15
HUT38678A (en) 1986-06-30
ES8609458A1 (es) 1986-09-01
FI94427B (fi) 1995-05-31
JP2502508B2 (ja) 1996-05-29
NO854312L (no) 1986-05-02
FI854142A0 (fi) 1985-10-23
JPH0698789A (ja) 1994-04-12
PL158965B1 (pl) 1992-10-30
JP2552808B2 (ja) 1996-11-13
AU572002B2 (en) 1988-04-28
JPH06233686A (ja) 1994-08-23

Similar Documents

Publication Publication Date Title
KR930001117B1 (ko) Dna 재조합 기술에 의한 폴리펩티드 제조방법
US4808537A (en) Methanol inducible genes obtained from pichia and methods of use
CA1283373C (en) Use of the gal 1 yeast promoter
KR0181179B1 (ko) 피치아 파스토리스 산성 인산효소 유전자의 디앤에이 단편 및 이를 사용하는 방법
EP0263311A2 (en) Yeast production of human tumor necrosis factor
EP0020147B1 (en) A dna transfer vector for human pre-growth hormone, a microorganism transformed thereby, and a method of cloning therefor
GB2091271A (en) Recombinant dna
US5795776A (en) Expression plasmids regulated by an OSMB promoter
JPH0253489A (ja) メチロトローフ酵母のアルコールオキシダーゼ２調節領域をコードするｄｎａ配列
EP0143834A1 (en) Heat regulated production of selected and fused proteins in yeast
IE81158B1 (en) Expression of hepatitis b s and pres2 proteins in methylotrophic yeasts
WO1984001153A1 (en) Production of interferon in yeast by use of invertase promoter
US5310660A (en) Method and a hybrid promoter for controlling exogenous gene transcription
KR100393297B1 (ko) 돌연변이체aox2프로모터,이를보유한벡터,형질전환체및이종단백질의제조방법
EP0339567A1 (en) Expression of Hepatitis B PreS 2 Protein in Methylotrophic Yeasts
US5217880A (en) L-fucose dehydrogenase gene, microorganism having said gene and production of l-fucose dehydrogenase by the use of said microorganism
CA2012025C (en) Dna for expression and secretion
US5496711A (en) Processes for producing pre-prorennin, prorennin and rennin
CA1156166A (en) Recombinant bacterial plasmids containing the coding sequences of insulin genes
CA1273883A (en) Recombinant dna material comprising bovine growth hormone nucleotide sequence
KR920001745B1 (ko) 인간성장호르몬 유전자 발현벡터[pYLBC-A/G-UB-HGH]와 그의 제조방법

Legal Events

Date Code Title Description
A201 Request for examination
E601 Decision to refuse application
E902 Notification of reason for refusal
J2X1 Appeal (before the patent court)

Free format text: APPEAL AGAINST DECISION TO DECLINE REFUSAL

G160 Decision to publish patent application
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20050107

Year of fee payment: 13

EXPY Expiration of term