MX368546B - Producción en alta pureza de proteínas multi-subunitarias tales como anticuerpos en microbios transformados tal como pichia pastoris. - Google Patents

Producción en alta pureza de proteínas multi-subunitarias tales como anticuerpos en microbios transformados tal como pichia pastoris.

Info

Publication number
MX368546B
MX368546B MX2013013532A MX2013013532A MX368546B MX 368546 B MX368546 B MX 368546B MX 2013013532 A MX2013013532 A MX 2013013532A MX 2013013532 A MX2013013532 A MX 2013013532A MX 368546 B MX368546 B MX 368546B
Authority
MX
Mexico
Prior art keywords
subunit proteins
pichia pastoris
antibodies
purity production
transformed microbes
Prior art date
Application number
MX2013013532A
Other languages
English (en)
Other versions
MX2013013532A (es
Inventor
F Garcia-Martinez Leon
A Latham John
Dianne Mcneil Patricia
Janson Nicole
L Lesnicki Gary
Qi Pei
Original Assignee
Alderbio Holdings Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alderbio Holdings Llc filed Critical Alderbio Holdings Llc
Publication of MX2013013532A publication Critical patent/MX2013013532A/es
Publication of MX368546B publication Critical patent/MX368546B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

Abstract

La presente invención se refiere a un método para producir un anticuerpo de longitud completa que comprende: (a) proporcionar un cultivo que comprende células de Pichia pastoris que comprenden genes que proporcionan la expresión del anticuerpo; (b) agregar un bolo de etanol al cultivo en una sola adición de bolo ("bolo") que resulta en una concentración de etanol en el cultivo entre 0.5% y 1.5%; y (c) cultivar el cultivo para producir el anticuerpo, con la condición de que los genes proporcionan la expresión del anticuerpo no están enlazados de manera operable a un promotor inducible con alcohol.
MX2013013532A 2011-05-20 2012-05-08 Producción en alta pureza de proteínas multi-subunitarias tales como anticuerpos en microbios transformados tal como pichia pastoris. MX368546B (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201161488660P 2011-05-20 2011-05-20
US201161496873P 2011-06-14 2011-06-14
US201161496860P 2011-06-14 2011-06-14
US201161525307P 2011-08-19 2011-08-19
PCT/US2012/036953 WO2012161956A2 (en) 2011-05-20 2012-05-08 High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris

Publications (2)

Publication Number Publication Date
MX2013013532A MX2013013532A (es) 2015-08-20
MX368546B true MX368546B (es) 2019-10-07

Family

ID=47217961

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2013013532A MX368546B (es) 2011-05-20 2012-05-08 Producción en alta pureza de proteínas multi-subunitarias tales como anticuerpos en microbios transformados tal como pichia pastoris.
MX2019012022A MX2019012022A (es) 2011-05-20 2013-11-19 Produccion en alta pureza de proteinas milti-subunitarias tales como anticuerpos en microbios transformados tal como pichia pastoris.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2019012022A MX2019012022A (es) 2011-05-20 2013-11-19 Produccion en alta pureza de proteinas milti-subunitarias tales como anticuerpos en microbios transformados tal como pichia pastoris.

Country Status (21)

Country Link
EP (1) EP2710114B1 (es)
JP (2) JP6193220B2 (es)
KR (1) KR101981662B1 (es)
CN (2) CN110408644A (es)
BR (1) BR112013029902A2 (es)
CA (1) CA2837000C (es)
CY (1) CY1124796T1 (es)
DK (1) DK2710114T3 (es)
EA (1) EA028913B9 (es)
ES (1) ES2899381T3 (es)
HR (1) HRP20211873T1 (es)
HU (1) HUE060418T2 (es)
IL (1) IL229430B (es)
LT (1) LT2710114T (es)
MX (2) MX368546B (es)
PL (1) PL2710114T3 (es)
PT (1) PT2710114T (es)
RS (1) RS62628B1 (es)
SG (1) SG194937A1 (es)
SI (1) SI2710114T1 (es)
WO (1) WO2012161956A2 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
UA123759C2 (uk) * 2014-03-21 2021-06-02 Тева Фармасьютікалз Інтернешнл Гмбх Застосування моноклонального антитіла, яке інгібує шлях пов'язаного з геном кальциноніну пептиду (cgrp), для лікування або зниження частоти випадків мігренозного головного болю у суб'єкта
TWI734775B (zh) * 2016-04-26 2021-08-01 美商美國泰福生技股份有限公司 細胞培養基
GB201803832D0 (en) * 2018-03-09 2018-04-25 Syndermix Ag Methods and compositions for lectin production
CN108913732B (zh) * 2018-07-31 2022-02-08 华东理工大学 一种莫纳可林j异源生产的方法及应用

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4855231A (en) 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
US4818700A (en) 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
US4812405A (en) 1986-02-18 1989-03-14 Phillips Petroleum Company Double auxotrophic mutants of Pichia pastoris and methods for preparation
US4929555A (en) 1987-10-19 1990-05-29 Phillips Petroleum Company Pichia transformation
WO1994021293A1 (en) 1993-03-19 1994-09-29 Duke University Method of treatment of tumors with an antibody binding to tenascin
AU3272695A (en) 1994-08-12 1996-03-07 Immunomedics Inc. Immunoconjugates and humanized antibodies specific for b-cell lymphoma and leukemia cells
US5955349A (en) 1996-08-26 1999-09-21 Zymogenetics, Inc. Compositions and methods for producing heterologous polypeptides in Pichia methanolica
US5736383A (en) 1996-08-26 1998-04-07 Zymogenetics, Inc. Preparation of Pichia methanolica auxotrophic mutants
US6653104B2 (en) 1996-10-17 2003-11-25 Immunomedics, Inc. Immunotoxins, comprising an internalizing antibody, directed against malignant and normal cells
US6730499B1 (en) 1998-07-03 2004-05-04 Research Corporation Technologies, Inc. Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1
WO2000056903A2 (en) * 1999-03-22 2000-09-28 Zymogenetics, Inc. IMPROVED METHODS FOR PRODUCING PROTEINS IN TRANSFORMED $i(PICHIA)
US6258559B1 (en) 1999-03-22 2001-07-10 Zymogenetics, Inc. Method for producing proteins in transformed Pichia
JP4494716B2 (ja) * 2000-12-13 2010-06-30 ユニリーバー・ナームローゼ・ベンノートシヤープ 菌類による異種たんぱく質の生産方法
WO2005040395A1 (en) 2003-10-22 2005-05-06 Keck Graduate Institute Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy
WO2008063302A2 (en) * 2006-10-10 2008-05-29 Keck Graduate Institute Novel p. pastoris pastoris promoters, and the use thereof to direct expression of proteins in yeast, preferably using a haploid mating strategy
EP2162469A4 (en) 2007-05-21 2012-08-01 Alderbio Holdings Llc NEW METHODS OF HUMANIZING RABBIT ANTIBODIES AND HUMANIZED RABBIT ANTIBODIES
HUE043782T2 (hu) 2007-05-21 2019-09-30 Alderbio Holdings Llc IL-6 elleni antitestek és alkalmazásuk
JP6190723B2 (ja) 2010-12-01 2017-08-30 アルダーバイオ ホールディングス エルエルシー 抗ｎｇｆ組成物およびその使用

Also Published As

Publication number Publication date
EP2710114A4 (en) 2014-11-19
EA028913B1 (ru) 2018-01-31
NZ718187A (en) 2017-10-27
WO2012161956A2 (en) 2012-11-29
EA201301294A1 (ru) 2014-05-30
SI2710114T1 (sl) 2022-01-31
RS62628B1 (sr) 2021-12-31
CN103827290A (zh) 2014-05-28
JP2018007656A (ja) 2018-01-18
EP2710114A2 (en) 2014-03-26
CA2837000C (en) 2021-09-14
SG194937A1 (en) 2013-12-30
KR20140033445A (ko) 2014-03-18
JP2014517698A (ja) 2014-07-24
MX2013013532A (es) 2015-08-20
HUE060418T2 (hu) 2023-02-28
BR112013029902A2 (pt) 2016-12-20
IL229430B (en) 2020-07-30
WO2012161956A3 (en) 2013-01-17
CN110408644A (zh) 2019-11-05
PL2710114T3 (pl) 2022-01-17
LT2710114T (lt) 2021-12-10
EA028913B9 (ru) 2018-03-30
CA2837000A1 (en) 2012-11-29
CY1124796T1 (el) 2022-11-25
IL229430A0 (en) 2014-01-30
PT2710114T (pt) 2021-12-09
DK2710114T3 (da) 2021-12-06
HRP20211873T1 (hr) 2022-03-04
KR101981662B1 (ko) 2019-05-23
JP6193220B2 (ja) 2017-09-06
ES2899381T3 (es) 2022-03-11
MX2019012022A (es) 2019-11-18
EP2710114B1 (en) 2021-11-03
JP6491696B2 (ja) 2019-03-27
NZ618635A (en) 2016-09-30
WO2012161956A9 (en) 2013-09-06

Similar Documents

Publication Publication Date Title
MX2019008417A (es) Cambio de temperatura para una expresion con mayor rendimiento de polipeptidos en levaduras y otras celulas transformadas.
MX2014001951A (es) Estrategia de multiples copias para produccion de alto titulo y alta pureza de proteinas de multi-subunidades tales como anticuerpos en microbios transformados tal como pichia pastoris.
WO2013060867A3 (en) Production of heterodimeric proteins
MY161726A (en) Biological methods for preparing adipic acid
EP3358000A3 (en) Protease deficient filamentous fungal cells and methods of use thereof
MX353144B (es) Proteinas que contienen fc de anticuerpos heterodimericos y metodos para produccion de las mismas.
MX343756B (es) Microorganismo que produce o-fosfoserina y metodo para producir l-cisteina o derivados de la misma a partir de o-fosfoserina usando los mismos.
MX355650B (es) Produccion de proteinas en medios de cultivo de celulas libres de glutamina.
MX2019003427A (es) Composiciones para cultivo celular y metodos para la produccion de polipeptidos.
WO2012068317A3 (en) Methods for producing recombinant proteins
CY1124796T1 (el) Παραγωγης υψηλης-καθαροτητας των πρωτεϊνων πολλαπλων-υπομοναδων οπως ειναι τα αντισωματα σε μετασχηματισθεντα μικροβια οπως ειναι to pichia pastoris
MX366112B (es) Medio de cultivo celular y metodos de produccion de anticuerpos.
IN2014CN02963A (es)
WO2014182684A3 (en) Methods and compositions related to large scale production of proteins
WO2012115903A3 (en) Yeast strain for the production of proteins with modified o-glycosylation
WO2014044626A3 (en) Process for producing gelatin employing aspergillopepsin ii
WO2013019604A3 (en) Protein production method
EP2925879A4 (en) IMPROVED CULTURE MEDIA AND PROCESS FOR PRODUCING PROTEIN ENHANCED BY PICHIA STRAINS
WO2015117033A3 (en) Methods of increasing protein production in mammalian cells
MY189384A (en) Biological methods for preparing adipic acid

Legal Events

Date Code Title Description
FG Grant or registration