MX368546B - Producción en alta pureza de proteínas multi-subunitarias tales como anticuerpos en microbios transformados tal como pichia pastoris. - Google Patents
Producción en alta pureza de proteínas multi-subunitarias tales como anticuerpos en microbios transformados tal como pichia pastoris.Info
- Publication number
- MX368546B MX368546B MX2013013532A MX2013013532A MX368546B MX 368546 B MX368546 B MX 368546B MX 2013013532 A MX2013013532 A MX 2013013532A MX 2013013532 A MX2013013532 A MX 2013013532A MX 368546 B MX368546 B MX 368546B
- Authority
- MX
- Mexico
- Prior art keywords
- subunit proteins
- pichia pastoris
- antibodies
- purity production
- transformed microbes
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
La presente invención se refiere a un método para producir un anticuerpo de longitud completa que comprende: (a) proporcionar un cultivo que comprende células de Pichia pastoris que comprenden genes que proporcionan la expresión del anticuerpo; (b) agregar un bolo de etanol al cultivo en una sola adición de bolo ("bolo") que resulta en una concentración de etanol en el cultivo entre 0.5% y 1.5%; y (c) cultivar el cultivo para producir el anticuerpo, con la condición de que los genes proporcionan la expresión del anticuerpo no están enlazados de manera operable a un promotor inducible con alcohol.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161488660P | 2011-05-20 | 2011-05-20 | |
| US201161496873P | 2011-06-14 | 2011-06-14 | |
| US201161496860P | 2011-06-14 | 2011-06-14 | |
| US201161525307P | 2011-08-19 | 2011-08-19 | |
| PCT/US2012/036953 WO2012161956A2 (en) | 2011-05-20 | 2012-05-08 | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2013013532A MX2013013532A (es) | 2015-08-20 |
| MX368546B true MX368546B (es) | 2019-10-07 |
Family
ID=47217961
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2013013532A MX368546B (es) | 2011-05-20 | 2012-05-08 | Producción en alta pureza de proteínas multi-subunitarias tales como anticuerpos en microbios transformados tal como pichia pastoris. |
| MX2019012022A MX2019012022A (es) | 2011-05-20 | 2013-11-19 | Produccion en alta pureza de proteinas milti-subunitarias tales como anticuerpos en microbios transformados tal como pichia pastoris. |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2019012022A MX2019012022A (es) | 2011-05-20 | 2013-11-19 | Produccion en alta pureza de proteinas milti-subunitarias tales como anticuerpos en microbios transformados tal como pichia pastoris. |
Country Status (21)
| Country | Link |
|---|---|
| EP (1) | EP2710114B1 (es) |
| JP (2) | JP6193220B2 (es) |
| KR (1) | KR101981662B1 (es) |
| CN (2) | CN110408644A (es) |
| BR (1) | BR112013029902A2 (es) |
| CA (1) | CA2837000C (es) |
| CY (1) | CY1124796T1 (es) |
| DK (1) | DK2710114T3 (es) |
| EA (1) | EA028913B9 (es) |
| ES (1) | ES2899381T3 (es) |
| HR (1) | HRP20211873T1 (es) |
| HU (1) | HUE060418T2 (es) |
| IL (1) | IL229430B (es) |
| LT (1) | LT2710114T (es) |
| MX (2) | MX368546B (es) |
| PL (1) | PL2710114T3 (es) |
| PT (1) | PT2710114T (es) |
| RS (1) | RS62628B1 (es) |
| SG (1) | SG194937A1 (es) |
| SI (1) | SI2710114T1 (es) |
| WO (1) | WO2012161956A2 (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RS65360B1 (sr) * | 2014-03-21 | 2024-04-30 | Teva Pharmaceuticals Int Gmbh | Antagonistička antitela specifična za peptid genski srodan kalcitoninu i postupci njihove upotrebe |
| TW202140776A (zh) * | 2016-04-26 | 2021-11-01 | 美商美國泰福生技股份有限公司 | 細胞培養基 |
| GB201803832D0 (en) * | 2018-03-09 | 2018-04-25 | Syndermix Ag | Methods and compositions for lectin production |
| CN108913732B (zh) * | 2018-07-31 | 2022-02-08 | 华东理工大学 | 一种莫纳可林j异源生产的方法及应用 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| US4812405A (en) | 1986-02-18 | 1989-03-14 | Phillips Petroleum Company | Double auxotrophic mutants of Pichia pastoris and methods for preparation |
| US4929555A (en) | 1987-10-19 | 1990-05-29 | Phillips Petroleum Company | Pichia transformation |
| WO1994021293A1 (en) | 1993-03-19 | 1994-09-29 | Duke University | Method of treatment of tumors with an antibody binding to tenascin |
| WO1996004925A1 (en) | 1994-08-12 | 1996-02-22 | Immunomedics, Inc. | Immunoconjugates and humanized antibodies specific for b-cell lymphoma and leukemia cells |
| US5955349A (en) | 1996-08-26 | 1999-09-21 | Zymogenetics, Inc. | Compositions and methods for producing heterologous polypeptides in Pichia methanolica |
| US5736383A (en) | 1996-08-26 | 1998-04-07 | Zymogenetics, Inc. | Preparation of Pichia methanolica auxotrophic mutants |
| US6653104B2 (en) | 1996-10-17 | 2003-11-25 | Immunomedics, Inc. | Immunotoxins, comprising an internalizing antibody, directed against malignant and normal cells |
| US6730499B1 (en) | 1998-07-03 | 2004-05-04 | Research Corporation Technologies, Inc. | Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1 |
| US6258559B1 (en) | 1999-03-22 | 2001-07-10 | Zymogenetics, Inc. | Method for producing proteins in transformed Pichia |
| AU3910000A (en) * | 1999-03-22 | 2000-10-09 | Zymogenetics Inc. | Improved methods for producing proteins in transformed (pichia) |
| CN1481439A (zh) * | 2000-12-13 | 2004-03-10 | 由真菌制备异源蛋白的方法 | |
| CN1871359B (zh) | 2003-10-22 | 2010-11-17 | 凯克研究生院 | 使用单倍体交配策略在酵母中合成异聚多亚基多肽的方法 |
| WO2008063302A2 (en) * | 2006-10-10 | 2008-05-29 | Keck Graduate Institute | Novel p. pastoris pastoris promoters, and the use thereof to direct expression of proteins in yeast, preferably using a haploid mating strategy |
| LT2164514T (lt) | 2007-05-21 | 2017-03-10 | Alderbio Holdings Llc | Il-6 antikūnai ir jų naudojimas |
| WO2008144757A1 (en) | 2007-05-21 | 2008-11-27 | Alder Biopharmaceuticals, Inc. | Novel rabbit antibody humanization methods and humanized rabbit antibodies |
| US9783602B2 (en) * | 2010-12-01 | 2017-10-10 | Alderbio Holdings Llc | Anti-NGF compositions and use thereof |
-
2012
- 2012-05-08 MX MX2013013532A patent/MX368546B/es active IP Right Grant
- 2012-05-08 SG SG2013084041A patent/SG194937A1/en unknown
- 2012-05-08 CA CA2837000A patent/CA2837000C/en active Active
- 2012-05-08 BR BR112013029902A patent/BR112013029902A2/pt not_active Application Discontinuation
- 2012-05-08 LT LTEPPCT/US2012/036953T patent/LT2710114T/lt unknown
- 2012-05-08 DK DK12789187.7T patent/DK2710114T3/da active
- 2012-05-08 PT PT127891877T patent/PT2710114T/pt unknown
- 2012-05-08 SI SI201231967T patent/SI2710114T1/sl unknown
- 2012-05-08 CN CN201910566412.4A patent/CN110408644A/zh active Pending
- 2012-05-08 EA EA201301294A patent/EA028913B9/ru not_active IP Right Cessation
- 2012-05-08 JP JP2014512858A patent/JP6193220B2/ja active Active
- 2012-05-08 CN CN201280035868.4A patent/CN103827290A/zh active Pending
- 2012-05-08 PL PL12789187T patent/PL2710114T3/pl unknown
- 2012-05-08 EP EP12789187.7A patent/EP2710114B1/en active Active
- 2012-05-08 HR HRP20211873TT patent/HRP20211873T1/hr unknown
- 2012-05-08 RS RS20211462A patent/RS62628B1/sr unknown
- 2012-05-08 WO PCT/US2012/036953 patent/WO2012161956A2/en active Application Filing
- 2012-05-08 KR KR1020137033842A patent/KR101981662B1/ko active IP Right Grant
- 2012-05-08 HU HUE12789187A patent/HUE060418T2/hu unknown
- 2012-05-08 ES ES12789187T patent/ES2899381T3/es active Active
-
2013
- 2013-11-14 IL IL229430A patent/IL229430B/en active IP Right Grant
- 2013-11-19 MX MX2019012022A patent/MX2019012022A/es unknown
-
2017
- 2017-06-19 JP JP2017119537A patent/JP6491696B2/ja active Active
-
2021
- 2021-12-02 CY CY20211101055T patent/CY1124796T1/el unknown
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |