CY1124796T1 - Παραγωγης υψηλης-καθαροτητας των πρωτεϊνων πολλαπλων-υπομοναδων οπως ειναι τα αντισωματα σε μετασχηματισθεντα μικροβια οπως ειναι to pichia pastoris - Google Patents
Παραγωγης υψηλης-καθαροτητας των πρωτεϊνων πολλαπλων-υπομοναδων οπως ειναι τα αντισωματα σε μετασχηματισθεντα μικροβια οπως ειναι to pichia pastorisInfo
- Publication number
- CY1124796T1 CY1124796T1 CY20211101055T CY211101055T CY1124796T1 CY 1124796 T1 CY1124796 T1 CY 1124796T1 CY 20211101055 T CY20211101055 T CY 20211101055T CY 211101055 T CY211101055 T CY 211101055T CY 1124796 T1 CY1124796 T1 CY 1124796T1
- Authority
- CY
- Cyprus
- Prior art keywords
- subunit proteins
- pichia pastoris
- antibodies
- purity production
- transformed microbes
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
Abstract
Μέθοδοι για παραγωγή ετερόλογων πρωτεϊνών πολλαπλών-υπομονάδων σε μετασχηματισθέντα κύτταρα αποκαλύπτονται. Ειδικότερα, η παρούσα αποκάλυψη παρέχει βελτιωμένες μεθόδους παραγωγής πρωτεϊνών πολλαπλών-υπο μονάδων που συμπεριλαμβάνουν αντισώματα και άλλες πρωτεΐνες πολλαπλών-υπομονάδων, τα οποία μπορεί ή δεν μπορεί να εκκριθούν, με μία υψηλότερη απόδοση και μειωμένη παραγωγή ανεπιθύμητων παραπροϊόντων. Σε επεξηγηματικές ως παραδείγματα ενσωματώσεις, τα μετασχηματισθέντα κύτταρα είναι ένας ζυμομύκητας, π.χ. μεθυλοτρόφος ζυμομύκητας όπως είναι το Pichia pastoris.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161488660P | 2011-05-20 | 2011-05-20 | |
US201161496873P | 2011-06-14 | 2011-06-14 | |
US201161496860P | 2011-06-14 | 2011-06-14 | |
US201161525307P | 2011-08-19 | 2011-08-19 | |
PCT/US2012/036953 WO2012161956A2 (en) | 2011-05-20 | 2012-05-08 | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris |
Publications (1)
Publication Number | Publication Date |
---|---|
CY1124796T1 true CY1124796T1 (el) | 2022-11-25 |
Family
ID=47217961
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CY20211101055T CY1124796T1 (el) | 2011-05-20 | 2021-12-02 | Παραγωγης υψηλης-καθαροτητας των πρωτεϊνων πολλαπλων-υπομοναδων οπως ειναι τα αντισωματα σε μετασχηματισθεντα μικροβια οπως ειναι to pichia pastoris |
Country Status (21)
Country | Link |
---|---|
EP (1) | EP2710114B1 (el) |
JP (2) | JP6193220B2 (el) |
KR (1) | KR101981662B1 (el) |
CN (2) | CN110408644A (el) |
BR (1) | BR112013029902A2 (el) |
CA (1) | CA2837000C (el) |
CY (1) | CY1124796T1 (el) |
DK (1) | DK2710114T3 (el) |
EA (1) | EA028913B9 (el) |
ES (1) | ES2899381T3 (el) |
HR (1) | HRP20211873T1 (el) |
HU (1) | HUE060418T2 (el) |
IL (1) | IL229430B (el) |
LT (1) | LT2710114T (el) |
MX (2) | MX368546B (el) |
PL (1) | PL2710114T3 (el) |
PT (1) | PT2710114T (el) |
RS (1) | RS62628B1 (el) |
SG (1) | SG194937A1 (el) |
SI (1) | SI2710114T1 (el) |
WO (1) | WO2012161956A2 (el) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
UA123759C2 (uk) * | 2014-03-21 | 2021-06-02 | Тева Фармасьютікалз Інтернешнл Гмбх | Застосування моноклонального антитіла, яке інгібує шлях пов'язаного з геном кальциноніну пептиду (cgrp), для лікування або зниження частоти випадків мігренозного головного болю у суб'єкта |
TWI734775B (zh) * | 2016-04-26 | 2021-08-01 | 美商美國泰福生技股份有限公司 | 細胞培養基 |
GB201803832D0 (en) * | 2018-03-09 | 2018-04-25 | Syndermix Ag | Methods and compositions for lectin production |
CN108913732B (zh) * | 2018-07-31 | 2022-02-08 | 华东理工大学 | 一种莫纳可林j异源生产的方法及应用 |
Family Cites Families (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
US4812405A (en) | 1986-02-18 | 1989-03-14 | Phillips Petroleum Company | Double auxotrophic mutants of Pichia pastoris and methods for preparation |
US4929555A (en) | 1987-10-19 | 1990-05-29 | Phillips Petroleum Company | Pichia transformation |
WO1994021293A1 (en) | 1993-03-19 | 1994-09-29 | Duke University | Method of treatment of tumors with an antibody binding to tenascin |
AU3272695A (en) | 1994-08-12 | 1996-03-07 | Immunomedics Inc. | Immunoconjugates and humanized antibodies specific for b-cell lymphoma and leukemia cells |
US5955349A (en) | 1996-08-26 | 1999-09-21 | Zymogenetics, Inc. | Compositions and methods for producing heterologous polypeptides in Pichia methanolica |
US5736383A (en) | 1996-08-26 | 1998-04-07 | Zymogenetics, Inc. | Preparation of Pichia methanolica auxotrophic mutants |
US6653104B2 (en) | 1996-10-17 | 2003-11-25 | Immunomedics, Inc. | Immunotoxins, comprising an internalizing antibody, directed against malignant and normal cells |
US6730499B1 (en) | 1998-07-03 | 2004-05-04 | Research Corporation Technologies, Inc. | Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1 |
WO2000056903A2 (en) * | 1999-03-22 | 2000-09-28 | Zymogenetics, Inc. | IMPROVED METHODS FOR PRODUCING PROTEINS IN TRANSFORMED $i(PICHIA) |
US6258559B1 (en) | 1999-03-22 | 2001-07-10 | Zymogenetics, Inc. | Method for producing proteins in transformed Pichia |
JP4494716B2 (ja) * | 2000-12-13 | 2010-06-30 | ユニリーバー・ナームローゼ・ベンノートシヤープ | 菌類による異種たんぱく質の生産方法 |
WO2005040395A1 (en) | 2003-10-22 | 2005-05-06 | Keck Graduate Institute | Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy |
WO2008063302A2 (en) * | 2006-10-10 | 2008-05-29 | Keck Graduate Institute | Novel p. pastoris pastoris promoters, and the use thereof to direct expression of proteins in yeast, preferably using a haploid mating strategy |
EP2162469A4 (en) | 2007-05-21 | 2012-08-01 | Alderbio Holdings Llc | NEW METHODS OF HUMANIZING RABBIT ANTIBODIES AND HUMANIZED RABBIT ANTIBODIES |
HUE043782T2 (hu) | 2007-05-21 | 2019-09-30 | Alderbio Holdings Llc | IL-6 elleni antitestek és alkalmazásuk |
JP6190723B2 (ja) | 2010-12-01 | 2017-08-30 | アルダーバイオ ホールディングス エルエルシー | 抗ngf組成物およびその使用 |
-
2012
- 2012-05-08 DK DK12789187.7T patent/DK2710114T3/da active
- 2012-05-08 CN CN201910566412.4A patent/CN110408644A/zh active Pending
- 2012-05-08 EA EA201301294A patent/EA028913B9/ru not_active IP Right Cessation
- 2012-05-08 JP JP2014512858A patent/JP6193220B2/ja active Active
- 2012-05-08 SI SI201231967T patent/SI2710114T1/sl unknown
- 2012-05-08 LT LTEPPCT/US2012/036953T patent/LT2710114T/lt unknown
- 2012-05-08 BR BR112013029902A patent/BR112013029902A2/pt not_active Application Discontinuation
- 2012-05-08 PT PT127891877T patent/PT2710114T/pt unknown
- 2012-05-08 HU HUE12789187A patent/HUE060418T2/hu unknown
- 2012-05-08 EP EP12789187.7A patent/EP2710114B1/en active Active
- 2012-05-08 WO PCT/US2012/036953 patent/WO2012161956A2/en active Application Filing
- 2012-05-08 KR KR1020137033842A patent/KR101981662B1/ko active IP Right Grant
- 2012-05-08 CA CA2837000A patent/CA2837000C/en active Active
- 2012-05-08 SG SG2013084041A patent/SG194937A1/en unknown
- 2012-05-08 ES ES12789187T patent/ES2899381T3/es active Active
- 2012-05-08 PL PL12789187T patent/PL2710114T3/pl unknown
- 2012-05-08 HR HRP20211873TT patent/HRP20211873T1/hr unknown
- 2012-05-08 RS RS20211462A patent/RS62628B1/sr unknown
- 2012-05-08 MX MX2013013532A patent/MX368546B/es active IP Right Grant
- 2012-05-08 CN CN201280035868.4A patent/CN103827290A/zh active Pending
-
2013
- 2013-11-14 IL IL229430A patent/IL229430B/en active IP Right Grant
- 2013-11-19 MX MX2019012022A patent/MX2019012022A/es unknown
-
2017
- 2017-06-19 JP JP2017119537A patent/JP6491696B2/ja active Active
-
2021
- 2021-12-02 CY CY20211101055T patent/CY1124796T1/el unknown
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CY1124796T1 (el) | Παραγωγης υψηλης-καθαροτητας των πρωτεϊνων πολλαπλων-υπομοναδων οπως ειναι τα αντισωματα σε μετασχηματισθεντα μικροβια οπως ειναι to pichia pastoris | |
MX2019008417A (es) | Cambio de temperatura para una expresion con mayor rendimiento de polipeptidos en levaduras y otras celulas transformadas. | |
CY1121862T1 (el) | Αντισωματα anti-phf-tau και χρησεις αυτων | |
CY1123774T1 (el) | Στελεχη shigella me υπερφυσαλιδωση | |
CY1119055T1 (el) | Αντισωματα που προσδενονται στην il-17a και στην il-17f | |
MX355890B (es) | Estrategia de múltiples copias para producción de alto título y alta pureza de proteínas de multi-subunidades tales como anticuerpos en microbios transformados tal como pichia pastoris. | |
CY1116940T1 (el) | Φλυκταινες εμπλουτισμενες σε φωσφολιπιδιο που φερουν παραγοντα ιστου εχουσες αιμοστατικες δραστικοτητες και χρησεις αυτων | |
CY1119173T1 (el) | Νεα μορια αναστολεα jnk | |
MX2016016886A (es) | Anticuerpos anti-axl. | |
EA201500142A1 (ru) | ШТАММ, ДЕФИЦИТНЫЙ ПО AgsE | |
MX368142B (es) | Metodo de clonacion, expresion y purificacion novedoso para la preparacion de ranibizumab. | |
EA201400938A1 (ru) | Способ получения 2-(2,3-диметилфенил)-1-пропаналя с использованием хлорацетона | |
BR112014026308A2 (pt) | composições de cultura celular e métodos para produção de polipeptídeos | |
MY181068A (en) | Recombinant microorganism for improved production of fine chemicals | |
BR112013027641A2 (pt) | processo verde para produzir poli-hidroxialcanoatos e produtos químicos com o uso de uma matéria-prima renovável | |
MX2016007729A (es) | Microorganismo recombinante para la produccion mejorada de productos quimicos finos. | |
CY1121055T1 (el) | Μεθοδος για την παρασκευη φορτισμενων διασυνδετων | |
CY1118539T1 (el) | Ανασυνδυασμενο μυκοβακτηριο ως εμβολιο | |
BR112015007830A2 (pt) | aumento do rendimento para plantas suscetíveis ao estresse | |
WO2014182684A3 (en) | Methods and compositions related to large scale production of proteins | |
WO2013152860A3 (en) | Improved antibody light chains | |
MX351610B (es) | Sales acidas de adicion de 5alfa-hidroxi-6beta-[2-(1h-imidazol-4-i l)etilamino]colestan-3beta-ol. | |
CY1121351T1 (el) | Μεθοδος για την παρασκευη οξυκωδονης | |
WO2012115903A3 (en) | Yeast strain for the production of proteins with modified o-glycosylation | |
CY1117407T1 (el) | Διαχωρισμος των εναντιομερων παραγωγων τριαζινης με χρηση τρυγικου οξεος |