MX368142B - Metodo de clonacion, expresion y purificacion novedoso para la preparacion de ranibizumab. - Google Patents

Metodo de clonacion, expresion y purificacion novedoso para la preparacion de ranibizumab.

Info

Publication number
MX368142B
MX368142B MX2015015188A MX2015015188A MX368142B MX 368142 B MX368142 B MX 368142B MX 2015015188 A MX2015015188 A MX 2015015188A MX 2015015188 A MX2015015188 A MX 2015015188A MX 368142 B MX368142 B MX 368142B
Authority
MX
Mexico
Prior art keywords
ranibizumab
expression
preparation
purification method
novel cloning
Prior art date
Application number
MX2015015188A
Other languages
English (en)
Other versions
MX2015015188A (es
Inventor
Shandilya Harish
Gadgil Himanshu
Farkade Vivek
Original Assignee
Intas Pharmaceuticals Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Intas Pharmaceuticals Ltd filed Critical Intas Pharmaceuticals Ltd
Publication of MX2015015188A publication Critical patent/MX2015015188A/es
Publication of MX368142B publication Critical patent/MX368142B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515—Complete light chain, i.e. VL + CL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a la clonación, expresión y producción de Ranibizumab utilizando un abordaje novedoso para una proteína de mejor rendimiento y biológicamente activa.
MX2015015188A 2013-04-30 2014-04-28 Metodo de clonacion, expresion y purificacion novedoso para la preparacion de ranibizumab. MX368142B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN1570MU2013 2013-04-30
PCT/IN2014/000274 WO2014178078A2 (en) 2013-04-30 2014-04-28 Novel cloning, expression & purification method for the preparation of ranibizumab

Publications (2)

Publication Number Publication Date
MX2015015188A MX2015015188A (es) 2016-09-07
MX368142B true MX368142B (es) 2019-09-20

Family

ID=51844062

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015015188A MX368142B (es) 2013-04-30 2014-04-28 Metodo de clonacion, expresion y purificacion novedoso para la preparacion de ranibizumab.

Country Status (7)

Country Link
US (1) US9914770B2 (es)
EP (1) EP2992021B1 (es)
CA (1) CA2911087A1 (es)
MX (1) MX368142B (es)
RU (1) RU2646098C2 (es)
WO (1) WO2014178078A2 (es)
ZA (1) ZA201508706B (es)

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US9840553B2 (en) 2014-06-28 2017-12-12 Kodiak Sciences Inc. Dual PDGF/VEGF antagonists
JP6748061B2 (ja) * 2014-07-09 2020-08-26 ルピン・リミテッド 二重シストロン細菌発現システム
PL3337820T3 (pl) * 2015-08-17 2021-11-22 Lupin Limited Ulepszony sposób ponownego fałdowania fragmentów przeciwciał
KR20250057128A (ko) 2015-12-30 2025-04-28 코디악 사이언시스 인코포레이티드 항체 및 이의 접합체
WO2018173075A1 (en) * 2017-03-24 2018-09-27 Council Of Scientific And Industrial Research A process for the purification of recombinant antibody fragments
JP7282689B2 (ja) * 2017-05-19 2023-05-29 カウンスィル オブ サイエンティフィック アンド インダストリアル リサーチ リフォールディングした組み換えヒト化ラニビズマブの製造方法
EP3727468A4 (en) 2017-12-19 2021-09-22 Akouos, Inc. AAV-MEDIATED THERAPEUTIC ANTIBODIES DELIVERY TO THE INTERNAL EAR
EP3758737A4 (en) 2018-03-02 2022-10-12 Kodiak Sciences Inc. IL-6 ANTIBODIES AND FUSION CONSTRUCTS AND CONJUGATES THEREOF
EP3836959A4 (en) 2018-08-17 2022-05-11 Trican Biotechnology Co., Ltd ANTI-ANGIOGENESIS FUSION PROTEIN AND USES ITS
CA3157509A1 (en) 2019-10-10 2021-04-15 Kodiak Sciences Inc. Methods of treating an eye disorder
WO2021072182A1 (en) * 2019-10-11 2021-04-15 Coherus Biosciences, Inc. Methods for producing ranibizumab
WO2021072210A1 (en) * 2019-10-11 2021-04-15 Coherus Biosciences, Inc. Methods of purifying ranibizumab or a ranibizumab variant
JP7638989B2 (ja) * 2019-11-21 2025-03-04 ユニティ バイオテクノロジー インコーポレイテッド Ｔｉｅ－２に対する抗体および使用方法
IL303317A (en) 2020-12-01 2023-07-01 Akouos Inc ANTI-NATURAL ANTIBODY STRUCTURES AND RELATED METHODS FOR THE TREATMENT OF SYMPTOMS ASSOCIATED WITH VESTIBULAR SWANNOMA
US12325752B2 (en) * 2020-12-18 2025-06-10 Regeneron Pharmaceuticals, Inc. Immunoglobulin proteins that bind to NPR1 agonists
EP4368203A4 (en) * 2021-07-05 2025-09-24 Wuhan Neurophth Biotechnology Ltd Company CONSTRUCTION AND USE OF ANTI-VEGF ANTIBODIES IN AN IN VIVO EXPRESSION SYSTEM
TW202342525A (zh) 2022-02-02 2023-11-01 美商阿科奧斯公司 用於治療前庭神經鞘瘤相關症狀之抗ｖｅｇｆ抗體構築體及相關方法

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AU4128089A (en) * 1988-09-15 1990-03-22 Rorer International (Overseas) Inc. Monoclonal antibodies specific to human epidermal growth factor receptor and therapeutic methods employing same
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
AU5134599A (en) * 1998-07-28 2000-02-21 California Institute Of Technology Expression of functional eukaryotic proteins
US6979556B2 (en) * 2000-12-14 2005-12-27 Genentech, Inc. Separate-cistron contructs for secretion of aglycosylated antibodies from prokaryotes
RU2420537C2 (ru) 2001-01-17 2011-06-10 Трабьон Фармасьютикалз Инк. Слитые белки связывающий домен-иммуноглобулин
WO2002092623A1 (en) 2001-05-11 2002-11-21 Research Development Foundation INHIBITORS OF RECEPTOR ACTIVATOR OF NF-κB AND USES THEREOF
DE10238846A1 (de) * 2002-08-20 2004-03-04 Nemod Immuntherapie Ag Aktive Fusionsproteine und Verfahren zu ihrer Herstellung
EP1581644B1 (en) * 2003-01-09 2007-06-06 Genentech, Inc. Purification of polypeptides
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HUE027902T2 (en) 2004-02-09 2016-11-28 Human Genome Sciences Inc Corp Service Company Albumin fusion proteins
JP4730302B2 (ja) 2004-04-20 2011-07-20 味の素株式会社 タンパク質の製造法
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Also Published As

Publication number Publication date
MX2015015188A (es) 2016-09-07
WO2014178078A2 (en) 2014-11-06
ZA201508706B (en) 2017-09-27
RU2015148746A (ru) 2017-06-05
EP2992021A4 (en) 2017-01-11
EP2992021A2 (en) 2016-03-09
WO2014178078A3 (en) 2014-12-24
CA2911087A1 (en) 2014-11-06
US9914770B2 (en) 2018-03-13
US20160289314A1 (en) 2016-10-06
RU2646098C2 (ru) 2018-03-01
EP2992021B1 (en) 2020-07-22

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