MX368142B - Metodo de clonacion, expresion y purificacion novedoso para la preparacion de ranibizumab. - Google Patents

Metodo de clonacion, expresion y purificacion novedoso para la preparacion de ranibizumab.

Info

Publication number
MX368142B
MX368142B MX2015015188A MX2015015188A MX368142B MX 368142 B MX368142 B MX 368142B MX 2015015188 A MX2015015188 A MX 2015015188A MX 2015015188 A MX2015015188 A MX 2015015188A MX 368142 B MX368142 B MX 368142B
Authority
MX
Mexico
Prior art keywords
ranibizumab
expression
preparation
purification method
novel cloning
Prior art date
Application number
MX2015015188A
Other languages
English (en)
Other versions
MX2015015188A (es
Inventor
Shandilya Harish
Gadgil Himanshu
Farkade Vivek
Original Assignee
Intas Pharmaceuticals Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Intas Pharmaceuticals Ltd filed Critical Intas Pharmaceuticals Ltd
Publication of MX2015015188A publication Critical patent/MX2015015188A/es
Publication of MX368142B publication Critical patent/MX368142B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515—Complete light chain, i.e. VL + CL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a la clonación, expresión y producción de Ranibizumab utilizando un abordaje novedoso para una proteína de mejor rendimiento y biológicamente activa.
MX2015015188A 2013-04-30 2014-04-28 Metodo de clonacion, expresion y purificacion novedoso para la preparacion de ranibizumab. MX368142B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN1570MU2013 2013-04-30
PCT/IN2014/000274 WO2014178078A2 (en) 2013-04-30 2014-04-28 Novel cloning, expression & purification method for the preparation of ranibizumab

Publications (2)

Publication Number Publication Date
MX2015015188A MX2015015188A (es) 2016-09-07
MX368142B true MX368142B (es) 2019-09-20

Family

ID=51844062

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015015188A MX368142B (es) 2013-04-30 2014-04-28 Metodo de clonacion, expresion y purificacion novedoso para la preparacion de ranibizumab.

Country Status (7)

Country Link
US (1) US9914770B2 (es)
EP (1) EP2992021B1 (es)
CA (1) CA2911087A1 (es)
MX (1) MX368142B (es)
RU (1) RU2646098C2 (es)
WO (1) WO2014178078A2 (es)
ZA (1) ZA201508706B (es)

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US9840553B2 (en) 2014-06-28 2017-12-12 Kodiak Sciences Inc. Dual PDGF/VEGF antagonists
CA2954594A1 (en) * 2014-07-09 2016-01-14 Lupin Limited Dual cistronic bacterial expression system
CN108026165A (zh) * 2015-08-17 2018-05-11 鲁平有限公司 用于抗体碎片的改进的复性方法
AU2016381964B2 (en) 2015-12-30 2024-02-15 Kodiak Sciences Inc. Antibodies and conjugates thereof
CN110582510B (zh) * 2017-03-24 2023-09-15 科学与工业研究委员会 用于纯化重组抗体片段的方法
BR112019023795A2 (pt) * 2017-05-19 2020-07-28 Council Of Scientific & Industrial Research método para produzir ranibizumabe humanizado recombinante redobrado
MY207644A (en) 2017-12-19 2025-03-07 Akouos Inc Aav-mediated delivery of therapeutic antibodies to the inner ear
CN112203679A (zh) 2018-03-02 2021-01-08 科达制药股份有限公司 Il-6抗体及其融合构建体和缀合物
JP7249060B2 (ja) 2018-08-17 2023-03-30 トリカン・バイオテクノロジー・カンパニー・リミテッド 抗血管新生融合タンパク質およびその使用
CA3157509A1 (en) 2019-10-10 2021-04-15 Kodiak Sciences Inc. Methods of treating an eye disorder
WO2021072182A1 (en) * 2019-10-11 2021-04-15 Coherus Biosciences, Inc. Methods for producing ranibizumab
WO2021072210A1 (en) * 2019-10-11 2021-04-15 Coherus Biosciences, Inc. Methods of purifying ranibizumab or a ranibizumab variant
AU2020385991A1 (en) * 2019-11-21 2022-06-23 Unity Biotechnology Antibodies directed to Tie-2 and methods of use
WO2022119839A1 (en) 2020-12-01 2022-06-09 Akouos, Inc. Anti-vegf antibody constructs and related methods for treating vestibular schwannoma associated symptoms
WO2023280157A1 (zh) * 2021-07-05 2023-01-12 武汉纽福斯生物科技有限公司 一种抗vegf抗体体内表达系统的构建和应用
AU2023215253A1 (en) 2022-02-02 2024-09-19 Akouos, Inc. Anti-vegf antibody constructs and related methods for treating vestibular schwannoma associated symptoms

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AU4128089A (en) * 1988-09-15 1990-03-22 Rorer International (Overseas) Inc. Monoclonal antibodies specific to human epidermal growth factor receptor and therapeutic methods employing same
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
MXPA01000224A (es) * 1998-07-28 2002-10-17 California Inst Of Techn Expresion de proteinas eucarioticas funcionales.
US6979556B2 (en) * 2000-12-14 2005-12-27 Genentech, Inc. Separate-cistron contructs for secretion of aglycosylated antibodies from prokaryotes
CN1911965B (zh) 2001-01-17 2013-05-29 新兴产品开发西雅图有限公司 结合域-免疫球蛋白融合蛋白
WO2002092623A1 (en) 2001-05-11 2002-11-21 Research Development Foundation INHIBITORS OF RECEPTOR ACTIVATOR OF NF-κB AND USES THEREOF
DE10238846A1 (de) * 2002-08-20 2004-03-04 Nemod Immuntherapie Ag Aktive Fusionsproteine und Verfahren zu ihrer Herstellung
DK1581644T3 (da) * 2003-01-09 2007-10-08 Genentech Inc Oprensning af polypeptider
GB0304576D0 (en) 2003-02-28 2003-04-02 Lonza Biologics Plc Protein a chromatography
EP1729795B1 (en) 2004-02-09 2016-02-03 Human Genome Sciences, Inc. Albumin fusion proteins
DK1748077T3 (da) 2004-04-20 2013-04-08 Ajinomoto Kk Fremgangsmåde til fremstilling af protein
WO2009021548A1 (en) * 2007-08-10 2009-02-19 Wacker Chemie Ag Expression of full length igg and secretion into the culture medium of prokaryotic cells
JP2011514161A (ja) * 2008-02-29 2011-05-06 ラモット・アット・テル・アビブ・ユニバーシテイ・リミテッド 免疫グロブリン組成物およびその製造方法
US20090311750A1 (en) * 2008-06-13 2009-12-17 West James W Methods of converting fab sequences into single chain antibody sequences
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Also Published As

Publication number Publication date
WO2014178078A3 (en) 2014-12-24
ZA201508706B (en) 2017-09-27
EP2992021B1 (en) 2020-07-22
EP2992021A4 (en) 2017-01-11
MX2015015188A (es) 2016-09-07
US9914770B2 (en) 2018-03-13
CA2911087A1 (en) 2014-11-06
RU2646098C2 (ru) 2018-03-01
RU2015148746A (ru) 2017-06-05
EP2992021A2 (en) 2016-03-09
US20160289314A1 (en) 2016-10-06
WO2014178078A2 (en) 2014-11-06

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