MX2014001951A - Estrategia de multiples copias para produccion de alto titulo y alta pureza de proteinas de multi-subunidades tales como anticuerpos en microbios transformados tal como pichia pastoris. - Google Patents
Estrategia de multiples copias para produccion de alto titulo y alta pureza de proteinas de multi-subunidades tales como anticuerpos en microbios transformados tal como pichia pastoris.Info
- Publication number
- MX2014001951A MX2014001951A MX2014001951A MX2014001951A MX2014001951A MX 2014001951 A MX2014001951 A MX 2014001951A MX 2014001951 A MX2014001951 A MX 2014001951A MX 2014001951 A MX2014001951 A MX 2014001951A MX 2014001951 A MX2014001951 A MX 2014001951A
- Authority
- MX
- Mexico
- Prior art keywords
- subunit proteins
- pichia pastoris
- antibodies
- titer
- purity production
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Se describen métodos para producir proteínas de múltiples subunidades heterólogas en células transformadas. En particular, la presente descripción proporciona métodos mejorados para producir proteínas de múltiples subunidades, incluyendo anticuerpos y otras proteínas de múltiples subunidades, que pueden o no secretarse, con un mayor rendimiento y una menor producción de subproductos indeseados. En ejemplos de modalidades, las células transformadas son una levadura, por ejemplo, levadura metilotrófica tal como Pichia pastoris.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161525307P | 2011-08-19 | 2011-08-19 | |
| US13/466,795 US11214610B2 (en) | 2010-12-01 | 2012-05-08 | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
| PCT/US2012/051619 WO2013028635A1 (en) | 2011-08-19 | 2012-08-20 | Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as a antibodies in transformed microbes such as pichia pastoris |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2014001951A true MX2014001951A (es) | 2016-02-05 |
| MX355890B MX355890B (es) | 2018-05-03 |
Family
ID=48802355
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2014001951A MX355890B (es) | 2011-08-19 | 2012-08-20 | Estrategia de múltiples copias para producción de alto título y alta pureza de proteínas de multi-subunidades tales como anticuerpos en microbios transformados tal como pichia pastoris. |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP2744903B1 (es) |
| JP (1) | JP6138788B2 (es) |
| KR (1) | KR101978534B1 (es) |
| CN (1) | CN103890178A (es) |
| AR (1) | AR087612A1 (es) |
| AU (1) | AU2012299035B2 (es) |
| BR (1) | BR112014003841B1 (es) |
| CA (1) | CA2845579C (es) |
| DK (1) | DK2744903T3 (es) |
| IL (1) | IL230978B (es) |
| MX (1) | MX355890B (es) |
| SG (2) | SG2014011878A (es) |
| TW (1) | TWI666318B (es) |
| WO (1) | WO2013028635A1 (es) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3597664A3 (en) * | 2013-03-15 | 2020-03-11 | Alder Biopharmaceuticals, Inc. | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells |
| US10202630B2 (en) | 2013-03-15 | 2019-02-12 | Alderbio Holdings Llc | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells |
| US9518082B2 (en) | 2013-03-15 | 2016-12-13 | Alderbio Holdings Llc | Antibody purification and purity monitoring |
| JP6206873B2 (ja) * | 2013-09-30 | 2017-10-04 | 国立研究開発法人農業・食品産業技術総合研究機構 | ニワトリリゾチーム由来の分泌シグナルペプチドを用いたブタリゾチームの製造方法 |
| US10556945B2 (en) | 2014-03-21 | 2020-02-11 | Teva Pharmaceuticals International Gmbh | Antagonist antibodies directed against calcitonin gene-related peptide and methods using same |
| KR102274964B1 (ko) | 2014-03-21 | 2021-07-09 | 테바 파마슈티컬스 인터내셔널 게엠베하 | 칼시토닌 유전자-관련 펩티드에 대한 길항제 항체 및 그의 사용 방법 |
| SG10201913032TA (en) | 2016-09-23 | 2020-02-27 | Teva Pharmaceuticals Int Gmbh | Treating refractory migraine |
| EP4202035A1 (en) | 2021-12-22 | 2023-06-28 | Gelita AG | Improved expression of peptides |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| US4812405A (en) | 1986-02-18 | 1989-03-14 | Phillips Petroleum Company | Double auxotrophic mutants of Pichia pastoris and methods for preparation |
| US4929555A (en) | 1987-10-19 | 1990-05-29 | Phillips Petroleum Company | Pichia transformation |
| WO1994021293A1 (en) | 1993-03-19 | 1994-09-29 | Duke University | Method of treatment of tumors with an antibody binding to tenascin |
| CA2195557C (en) | 1994-08-12 | 2006-10-17 | Shui-On Leung | Immunoconjugates and humanized antibodies specific for b-cell lymphoma and leukemia cells |
| US5955349A (en) | 1996-08-26 | 1999-09-21 | Zymogenetics, Inc. | Compositions and methods for producing heterologous polypeptides in Pichia methanolica |
| US5736383A (en) | 1996-08-26 | 1998-04-07 | Zymogenetics, Inc. | Preparation of Pichia methanolica auxotrophic mutants |
| US6730499B1 (en) | 1998-07-03 | 2004-05-04 | Research Corporation Technologies, Inc. | Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1 |
| WO2000023579A1 (en) | 1998-10-22 | 2000-04-27 | The Regents Of The University Of California | Functionally assembled antigen-specific intact recombinant antibody and a method for production thereof |
| US6258559B1 (en) | 1999-03-22 | 2001-07-10 | Zymogenetics, Inc. | Method for producing proteins in transformed Pichia |
| US6406863B1 (en) * | 2000-06-23 | 2002-06-18 | Genetastix Corporation | High throughput generation and screening of fully human antibody repertoire in yeast |
| US8268582B2 (en) * | 2003-10-22 | 2012-09-18 | Keck Graduate Institute | Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy |
| US8222386B2 (en) | 2006-10-10 | 2012-07-17 | Keck Graduate Institute | P. pastoris ADH promoter and use thereof to direct expression of proteins |
| TWI609965B (zh) | 2007-05-21 | 2018-01-01 | 艾爾德生物控股有限責任公司 | 新穎兔抗體人化方法以及經人化之兔抗體 |
| SI2164514T1 (sl) * | 2007-05-21 | 2017-04-26 | Alderbio Holdings Llc | Protitelesa proti IL-6 in njihova uporaba |
| US11214610B2 (en) | 2010-12-01 | 2022-01-04 | H. Lundbeck A/S | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
-
2012
- 2012-08-20 JP JP2014527227A patent/JP6138788B2/ja active Active
- 2012-08-20 SG SG2014011878A patent/SG2014011878A/en unknown
- 2012-08-20 TW TW101130127A patent/TWI666318B/zh not_active IP Right Cessation
- 2012-08-20 CA CA2845579A patent/CA2845579C/en active Active
- 2012-08-20 WO PCT/US2012/051619 patent/WO2013028635A1/en active Application Filing
- 2012-08-20 BR BR112014003841-4A patent/BR112014003841B1/pt active IP Right Grant
- 2012-08-20 EP EP12825119.6A patent/EP2744903B1/en active Active
- 2012-08-20 AU AU2012299035A patent/AU2012299035B2/en active Active
- 2012-08-20 CN CN201280051222.5A patent/CN103890178A/zh active Pending
- 2012-08-20 KR KR1020147007256A patent/KR101978534B1/ko active IP Right Grant
- 2012-08-20 SG SG10201609752YA patent/SG10201609752YA/en unknown
- 2012-08-20 MX MX2014001951A patent/MX355890B/es active IP Right Grant
- 2012-08-20 DK DK12825119.6T patent/DK2744903T3/en active
- 2012-08-21 AR ARP120103067A patent/AR087612A1/es unknown
-
2014
- 2014-02-13 IL IL230978A patent/IL230978B/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| TW201313899A (zh) | 2013-04-01 |
| CA2845579A1 (en) | 2013-02-28 |
| EP2744903A4 (en) | 2014-12-31 |
| IL230978A0 (en) | 2014-03-31 |
| NZ621199A (en) | 2016-07-29 |
| EP2744903A1 (en) | 2014-06-25 |
| WO2013028635A1 (en) | 2013-02-28 |
| IL230978B (en) | 2018-08-30 |
| JP6138788B2 (ja) | 2017-05-31 |
| SG2014011878A (en) | 2014-04-28 |
| AR087612A1 (es) | 2014-04-03 |
| BR112014003841A2 (pt) | 2017-03-14 |
| BR112014003841B1 (pt) | 2022-02-15 |
| CN103890178A (zh) | 2014-06-25 |
| SG10201609752YA (en) | 2017-01-27 |
| NZ721102A (en) | 2020-01-31 |
| KR101978534B1 (ko) | 2019-08-28 |
| EP2744903B1 (en) | 2018-10-10 |
| CA2845579C (en) | 2020-03-24 |
| TWI666318B (zh) | 2019-07-21 |
| DK2744903T3 (en) | 2019-01-28 |
| JP2014525247A (ja) | 2014-09-29 |
| WO2013028635A9 (en) | 2013-09-26 |
| MX355890B (es) | 2018-05-03 |
| AU2012299035A1 (en) | 2014-03-13 |
| AU2012299035B2 (en) | 2017-11-02 |
| KR20140057613A (ko) | 2014-05-13 |
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Legal Events
| Date | Code | Title | Description |
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| FG | Grant or registration |