AR087612A1 - Estrategia multicopia para producir proteinas, tales como anticuerpos con titulacion y pureza elevadas, en microbios transformados, tales como pichia pastoris - Google Patents
Estrategia multicopia para producir proteinas, tales como anticuerpos con titulacion y pureza elevadas, en microbios transformados, tales como pichia pastorisInfo
- Publication number
- AR087612A1 AR087612A1 ARP120103067A ARP120103067A AR087612A1 AR 087612 A1 AR087612 A1 AR 087612A1 AR P120103067 A ARP120103067 A AR P120103067A AR P120103067 A ARP120103067 A AR P120103067A AR 087612 A1 AR087612 A1 AR 087612A1
- Authority
- AR
- Argentina
- Prior art keywords
- complex
- multiple subunits
- host cells
- cells
- produced
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Métodos para producir proteínas heterólogas con múltiples subunidades en células transformadas. Métodos mejorados para producir proteínas con múltiples subunidades tales como anticuerpos y otras proteínas con múltiples subunidades, que pueden ser secretadas o no. con un rendimiento elevado y con una menor cantidad de productos colaterales indeseables. En diversos ejemplos de formas de realización, las células transformadas son levaduras, por ejemplo, levaduras metilotróficas, tales como Pichia pastoris. Reivindicación 1: Un método para identificar una célula huésped en la que se produce un complejo con múltiples subunidades deseado con un rendimiento superior, caracterizado porque comprende (a) proveer un panel de células huésped, donde dicho panel comprende al menos dos células huésped, cada una de las cuales comprende genes a partir de los cuales pueden expresarse las subunidades del complejo con múltiples subunidades; donde dichas al menos dos células huésped se producen apareando o fusionando dos células progenitoras, donde cada célula progenitora comprende una o más copias de al menos un gen que codifica una subunidad del complejo con múltiples subunidades; (b) cultivar cada una de las células huésped para que se exprese el complejo con múltiples subunidades, donde los genes en las al menos dos células huésped dan como resultado niveles de expresión diferentes de al menos una subunidad del complejo con múltiples subunidades; (c) medir el rendimiento del complejo con múltiples subunidades que se produce en cada una de las células huésped; y (d) identificar la célula huésped en la que se produce un rendimiento superior al del resto de las células huésped en el panel como una célula huésped en la que se produce el complejo con múltiples subunidades deseado con un rendimiento superior.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161525307P | 2011-08-19 | 2011-08-19 | |
| US13/466,795 US11214610B2 (en) | 2010-12-01 | 2012-05-08 | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR087612A1 true AR087612A1 (es) | 2014-04-03 |
Family
ID=48802355
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP120103067A AR087612A1 (es) | 2011-08-19 | 2012-08-21 | Estrategia multicopia para producir proteinas, tales como anticuerpos con titulacion y pureza elevadas, en microbios transformados, tales como pichia pastoris |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP2744903B1 (es) |
| JP (1) | JP6138788B2 (es) |
| KR (1) | KR101978534B1 (es) |
| CN (1) | CN103890178A (es) |
| AR (1) | AR087612A1 (es) |
| AU (1) | AU2012299035B2 (es) |
| BR (1) | BR112014003841B1 (es) |
| CA (1) | CA2845579C (es) |
| DK (1) | DK2744903T3 (es) |
| IL (1) | IL230978B (es) |
| MX (1) | MX355890B (es) |
| SG (2) | SG10201609752YA (es) |
| TW (1) | TWI666318B (es) |
| WO (1) | WO2013028635A1 (es) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3754012A1 (en) * | 2013-03-15 | 2020-12-23 | Alder Biopharmaceuticals, Inc. | Antibody purification and purity monitoring |
| US10202630B2 (en) | 2013-03-15 | 2019-02-12 | Alderbio Holdings Llc | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells |
| KR102202477B1 (ko) * | 2013-03-15 | 2021-01-13 | 룬드벡 시애틀 바이오파마슈티컬즈, 인크. | 효모 및 기타 형질전환 세포에서 폴리펩티드의 고수율 발현을 위한 온도 전환 |
| JP6206873B2 (ja) * | 2013-09-30 | 2017-10-04 | 国立研究開発法人農業・食品産業技術総合研究機構 | ニワトリリゾチーム由来の分泌シグナルペプチドを用いたブタリゾチームの製造方法 |
| RS65360B1 (sr) | 2014-03-21 | 2024-04-30 | Teva Pharmaceuticals Int Gmbh | Antagonistička antitela specifična za peptid genski srodan kalcitoninu i postupci njihove upotrebe |
| US10556945B2 (en) | 2014-03-21 | 2020-02-11 | Teva Pharmaceuticals International Gmbh | Antagonist antibodies directed against calcitonin gene-related peptide and methods using same |
| MX2019003337A (es) | 2016-09-23 | 2019-09-26 | Teva Pharmaceuticals Int Gmbh | Tratamiento para migraña refractaria. |
| EP4202035A1 (en) | 2021-12-22 | 2023-06-28 | Gelita AG | Improved expression of peptides |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| US4812405A (en) | 1986-02-18 | 1989-03-14 | Phillips Petroleum Company | Double auxotrophic mutants of Pichia pastoris and methods for preparation |
| US4929555A (en) | 1987-10-19 | 1990-05-29 | Phillips Petroleum Company | Pichia transformation |
| WO1994021293A1 (en) | 1993-03-19 | 1994-09-29 | Duke University | Method of treatment of tumors with an antibody binding to tenascin |
| WO1996004925A1 (en) | 1994-08-12 | 1996-02-22 | Immunomedics, Inc. | Immunoconjugates and humanized antibodies specific for b-cell lymphoma and leukemia cells |
| US5955349A (en) | 1996-08-26 | 1999-09-21 | Zymogenetics, Inc. | Compositions and methods for producing heterologous polypeptides in Pichia methanolica |
| US5736383A (en) | 1996-08-26 | 1998-04-07 | Zymogenetics, Inc. | Preparation of Pichia methanolica auxotrophic mutants |
| US6730499B1 (en) | 1998-07-03 | 2004-05-04 | Research Corporation Technologies, Inc. | Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1 |
| AU1219000A (en) | 1998-10-22 | 2000-05-08 | Regents Of The University Of California, The | Functionally assembled antigen-specific intact recombinant antibody and a methodfor production thereof |
| US6258559B1 (en) | 1999-03-22 | 2001-07-10 | Zymogenetics, Inc. | Method for producing proteins in transformed Pichia |
| US6406863B1 (en) * | 2000-06-23 | 2002-06-18 | Genetastix Corporation | High throughput generation and screening of fully human antibody repertoire in yeast |
| CN1871359B (zh) | 2003-10-22 | 2010-11-17 | 凯克研究生院 | 使用单倍体交配策略在酵母中合成异聚多亚基多肽的方法 |
| WO2008063302A2 (en) | 2006-10-10 | 2008-05-29 | Keck Graduate Institute | Novel p. pastoris pastoris promoters, and the use thereof to direct expression of proteins in yeast, preferably using a haploid mating strategy |
| LT2164514T (lt) | 2007-05-21 | 2017-03-10 | Alderbio Holdings Llc | Il-6 antikūnai ir jų naudojimas |
| WO2008144757A1 (en) | 2007-05-21 | 2008-11-27 | Alder Biopharmaceuticals, Inc. | Novel rabbit antibody humanization methods and humanized rabbit antibodies |
| US11214610B2 (en) | 2010-12-01 | 2022-01-04 | H. Lundbeck A/S | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
-
2012
- 2012-08-20 CN CN201280051222.5A patent/CN103890178A/zh active Pending
- 2012-08-20 WO PCT/US2012/051619 patent/WO2013028635A1/en active Application Filing
- 2012-08-20 MX MX2014001951A patent/MX355890B/es active IP Right Grant
- 2012-08-20 SG SG10201609752YA patent/SG10201609752YA/en unknown
- 2012-08-20 DK DK12825119.6T patent/DK2744903T3/en active
- 2012-08-20 TW TW101130127A patent/TWI666318B/zh not_active IP Right Cessation
- 2012-08-20 BR BR112014003841-4A patent/BR112014003841B1/pt active IP Right Grant
- 2012-08-20 SG SG2014011878A patent/SG2014011878A/en unknown
- 2012-08-20 KR KR1020147007256A patent/KR101978534B1/ko active IP Right Grant
- 2012-08-20 EP EP12825119.6A patent/EP2744903B1/en active Active
- 2012-08-20 CA CA2845579A patent/CA2845579C/en active Active
- 2012-08-20 JP JP2014527227A patent/JP6138788B2/ja active Active
- 2012-08-20 AU AU2012299035A patent/AU2012299035B2/en active Active
- 2012-08-21 AR ARP120103067A patent/AR087612A1/es unknown
-
2014
- 2014-02-13 IL IL230978A patent/IL230978B/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| SG2014011878A (en) | 2014-04-28 |
| WO2013028635A1 (en) | 2013-02-28 |
| MX2014001951A (es) | 2016-02-05 |
| EP2744903B1 (en) | 2018-10-10 |
| CA2845579C (en) | 2020-03-24 |
| TWI666318B (zh) | 2019-07-21 |
| JP6138788B2 (ja) | 2017-05-31 |
| CN103890178A (zh) | 2014-06-25 |
| AU2012299035B2 (en) | 2017-11-02 |
| NZ721102A (en) | 2020-01-31 |
| BR112014003841B1 (pt) | 2022-02-15 |
| IL230978A0 (en) | 2014-03-31 |
| MX355890B (es) | 2018-05-03 |
| IL230978B (en) | 2018-08-30 |
| EP2744903A4 (en) | 2014-12-31 |
| AU2012299035A1 (en) | 2014-03-13 |
| DK2744903T3 (en) | 2019-01-28 |
| NZ621199A (en) | 2016-07-29 |
| KR101978534B1 (ko) | 2019-08-28 |
| SG10201609752YA (en) | 2017-01-27 |
| CA2845579A1 (en) | 2013-02-28 |
| EP2744903A1 (en) | 2014-06-25 |
| WO2013028635A9 (en) | 2013-09-26 |
| BR112014003841A2 (pt) | 2017-03-14 |
| KR20140057613A (ko) | 2014-05-13 |
| JP2014525247A (ja) | 2014-09-29 |
| TW201313899A (zh) | 2013-04-01 |
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