SG10201609752YA - Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris - Google Patents
Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastorisInfo
- Publication number
- SG10201609752YA SG10201609752YA SG10201609752YA SG10201609752YA SG10201609752YA SG 10201609752Y A SG10201609752Y A SG 10201609752YA SG 10201609752Y A SG10201609752Y A SG 10201609752YA SG 10201609752Y A SG10201609752Y A SG 10201609752YA SG 10201609752Y A SG10201609752Y A SG 10201609752YA
- Authority
- SG
- Singapore
- Prior art keywords
- titer
- antibodies
- pichia pastoris
- subunit proteins
- purity production
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Botany (AREA)
- Immunology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161525307P | 2011-08-19 | 2011-08-19 | |
| US13/466,795 US11214610B2 (en) | 2010-12-01 | 2012-05-08 | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG10201609752YA true SG10201609752YA (en) | 2017-01-27 |
Family
ID=48802355
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10201609752YA SG10201609752YA (en) | 2011-08-19 | 2012-08-20 | Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris |
| SG2014011878A SG2014011878A (en) | 2011-08-19 | 2012-08-20 | Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as a antibodies in transformed microbes such as pichia pastoris |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG2014011878A SG2014011878A (en) | 2011-08-19 | 2012-08-20 | Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as a antibodies in transformed microbes such as pichia pastoris |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP2744903B1 (es) |
| JP (1) | JP6138788B2 (es) |
| KR (1) | KR101978534B1 (es) |
| CN (1) | CN103890178A (es) |
| AR (1) | AR087612A1 (es) |
| AU (1) | AU2012299035B2 (es) |
| BR (1) | BR112014003841B1 (es) |
| CA (1) | CA2845579C (es) |
| DK (1) | DK2744903T3 (es) |
| IL (1) | IL230978B (es) |
| MX (1) | MX355890B (es) |
| SG (2) | SG10201609752YA (es) |
| TW (1) | TWI666318B (es) |
| WO (1) | WO2013028635A1 (es) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102202477B1 (ko) * | 2013-03-15 | 2021-01-13 | 룬드벡 시애틀 바이오파마슈티컬즈, 인크. | 효모 및 기타 형질전환 세포에서 폴리펩티드의 고수율 발현을 위한 온도 전환 |
| TW201940691A (zh) * | 2013-03-15 | 2019-10-16 | 美商艾爾德生物製藥股份有限公司 | 抗體純化及純度監測 |
| US10202630B2 (en) | 2013-03-15 | 2019-02-12 | Alderbio Holdings Llc | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells |
| JP6206873B2 (ja) * | 2013-09-30 | 2017-10-04 | 国立研究開発法人農業・食品産業技術総合研究機構 | ニワトリリゾチーム由来の分泌シグナルペプチドを用いたブタリゾチームの製造方法 |
| US10556945B2 (en) | 2014-03-21 | 2020-02-11 | Teva Pharmaceuticals International Gmbh | Antagonist antibodies directed against calcitonin gene-related peptide and methods using same |
| JP6568099B2 (ja) | 2014-03-21 | 2019-08-28 | テバ・ファーマシューティカルズ・インターナショナル・ゲーエムベーハーＴｅｖａ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ Ｉｎｔｅｒｎａｔｉｏｎａｌ ＧｍｂＨ | カルシトニン遺伝子関連ペプチドに対するアンタゴニスト抗体及びその使用方法 |
| CA3036632A1 (en) | 2016-09-23 | 2018-03-29 | Teva Pharmaceuticals International Gmbh | Treating refractory migraine |
| EP4202035A1 (en) | 2021-12-22 | 2023-06-28 | Gelita AG | Improved expression of peptides |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| US4812405A (en) | 1986-02-18 | 1989-03-14 | Phillips Petroleum Company | Double auxotrophic mutants of Pichia pastoris and methods for preparation |
| US4929555A (en) | 1987-10-19 | 1990-05-29 | Phillips Petroleum Company | Pichia transformation |
| AU6445894A (en) | 1993-03-19 | 1994-10-11 | Duke University | Method of treatment of tumors with an antibody binding to tenascin |
| ATE306930T1 (de) | 1994-08-12 | 2005-11-15 | Immunomedics Inc | Für b-zell-lymphom und leukämiezellen spezifische immunkonjugate und humane antikörper |
| US5955349A (en) | 1996-08-26 | 1999-09-21 | Zymogenetics, Inc. | Compositions and methods for producing heterologous polypeptides in Pichia methanolica |
| US5736383A (en) | 1996-08-26 | 1998-04-07 | Zymogenetics, Inc. | Preparation of Pichia methanolica auxotrophic mutants |
| US6730499B1 (en) | 1998-07-03 | 2004-05-04 | Research Corporation Technologies, Inc. | Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1 |
| WO2000023579A1 (en) | 1998-10-22 | 2000-04-27 | The Regents Of The University Of California | Functionally assembled antigen-specific intact recombinant antibody and a method for production thereof |
| US6258559B1 (en) | 1999-03-22 | 2001-07-10 | Zymogenetics, Inc. | Method for producing proteins in transformed Pichia |
| US6406863B1 (en) * | 2000-06-23 | 2002-06-18 | Genetastix Corporation | High throughput generation and screening of fully human antibody repertoire in yeast |
| CN1871359B (zh) * | 2003-10-22 | 2010-11-17 | 凯克研究生院 | 使用单倍体交配策略在酵母中合成异聚多亚基多肽的方法 |
| WO2008063302A2 (en) * | 2006-10-10 | 2008-05-29 | Keck Graduate Institute | Novel p. pastoris pastoris promoters, and the use thereof to direct expression of proteins in yeast, preferably using a haploid mating strategy |
| NZ601583A (en) | 2007-05-21 | 2013-08-30 | Bristol Myers Squibb Co | Novel rabbit antibody humanization methods and humanized rabbit antibodies |
| CA2688146C (en) * | 2007-05-21 | 2018-03-06 | Alder Biopharmaceuticals, Inc. | Antibodies to il-6 and use thereof |
| US11214610B2 (en) | 2010-12-01 | 2022-01-04 | H. Lundbeck A/S | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
-
2012
- 2012-08-20 MX MX2014001951A patent/MX355890B/es active IP Right Grant
- 2012-08-20 DK DK12825119.6T patent/DK2744903T3/en active
- 2012-08-20 WO PCT/US2012/051619 patent/WO2013028635A1/en active Application Filing
- 2012-08-20 SG SG10201609752YA patent/SG10201609752YA/en unknown
- 2012-08-20 BR BR112014003841-4A patent/BR112014003841B1/pt active IP Right Grant
- 2012-08-20 TW TW101130127A patent/TWI666318B/zh not_active IP Right Cessation
- 2012-08-20 CA CA2845579A patent/CA2845579C/en active Active
- 2012-08-20 KR KR1020147007256A patent/KR101978534B1/ko active IP Right Grant
- 2012-08-20 SG SG2014011878A patent/SG2014011878A/en unknown
- 2012-08-20 CN CN201280051222.5A patent/CN103890178A/zh active Pending
- 2012-08-20 AU AU2012299035A patent/AU2012299035B2/en active Active
- 2012-08-20 JP JP2014527227A patent/JP6138788B2/ja active Active
- 2012-08-20 EP EP12825119.6A patent/EP2744903B1/en active Active
- 2012-08-21 AR ARP120103067A patent/AR087612A1/es unknown
-
2014
- 2014-02-13 IL IL230978A patent/IL230978B/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| CA2845579A1 (en) | 2013-02-28 |
| CN103890178A (zh) | 2014-06-25 |
| KR20140057613A (ko) | 2014-05-13 |
| AU2012299035A1 (en) | 2014-03-13 |
| CA2845579C (en) | 2020-03-24 |
| NZ621199A (en) | 2016-07-29 |
| KR101978534B1 (ko) | 2019-08-28 |
| NZ721102A (en) | 2020-01-31 |
| AU2012299035B2 (en) | 2017-11-02 |
| BR112014003841A2 (pt) | 2017-03-14 |
| WO2013028635A9 (en) | 2013-09-26 |
| JP2014525247A (ja) | 2014-09-29 |
| IL230978A0 (en) | 2014-03-31 |
| TW201313899A (zh) | 2013-04-01 |
| EP2744903A4 (en) | 2014-12-31 |
| MX355890B (es) | 2018-05-03 |
| IL230978B (en) | 2018-08-30 |
| JP6138788B2 (ja) | 2017-05-31 |
| TWI666318B (zh) | 2019-07-21 |
| EP2744903B1 (en) | 2018-10-10 |
| MX2014001951A (es) | 2016-02-05 |
| AR087612A1 (es) | 2014-04-03 |
| BR112014003841B1 (pt) | 2022-02-15 |
| EP2744903A1 (en) | 2014-06-25 |
| SG2014011878A (en) | 2014-04-28 |
| WO2013028635A1 (en) | 2013-02-28 |
| DK2744903T3 (en) | 2019-01-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| IL230978B (en) | A multi-copy strategy for high titer and high purity production of multi-subunit proteins such as antibodies in transformed bacteria such as P. pasteuris | |
| HK1247628A1 (zh) | 多特異性fab融合蛋白及其使用方法 | |
| HK1214277A1 (zh) | 生產異源多聚體蛋白質 | |
| GB201213868D0 (en) | Improvements in or relating to methods of manufacture | |
| EP2582729A4 (en) | ANTI-AXL ANTIBODIES AND METHOD FOR THEIR USE | |
| PT2768857T (pt) | Métodos para purificar anticorpos | |
| LT3111954T (lt) | Ankilozuojančio spondilito gydymo būdai, naudojant anti-il-17 antikūnus | |
| GB201104585D0 (en) | Composition and method of manufacture | |
| PL2785701T3 (pl) | Krystaliczna postać kabazytakselu i sposób jej otrzymywania | |
| SG10201604562PA (en) | Biotechnological production of 3-hydroxyisobutyric acid | |
| EP2747975A4 (en) | MANUFACTURED ARTICLE AND PROCESS FOR PREPARING THE SAME | |
| EP2691385A4 (en) | IMPROVED METHOD FOR THE PRODUCTION OF IMATINIB AND ITS MESYLATE SALT | |
| IL229430A0 (en) | A highly purified production of multi-subunit proteins such as antibodies in genetically engineered microorganisms such as Pichia pastoris | |
| EP2814960A4 (en) | USE OF IDED NANOSTRUCTURES IN NUCLEIC ACID TECHNOLOGY | |
| GB201114432D0 (en) | Container and method of manufacture of container | |
| EP2445531A4 (en) | PROCESS FOR INCREASED NEURONAL DIFFERENTIATION USING ANTIBODIES TO LYSOPHOSPHACIDIC ACID | |
| EP2712366A4 (en) | METHOD FOR IMPREGNATING AND PURIFYING POLY RESIN (LACTIC ACID) | |
| EP2574209A4 (en) | MANIPULATION OF SURFACE PEPPERS TO IMPROVE PROTEIN CRYSTALLIZATION | |
| HUP1000638A2 (en) | Process for preparation of rosuvastatin salts | |
| ZA201404666B (en) | Process for preparing and purifying salts of acrylamido-2-methylpropanesulfonic acid | |
| PL395836A1 (pl) | Sposób wytwarzania wkladu do swiec w pojemnikach | |
| EP2578091A4 (en) | PROCESS FOR IMPROVING THE STABILITY IN PRESERVATION OF A PROTEINED BEVERAGE | |
| GB201006582D0 (en) | Container and method of manufacture therof | |
| HUP1200589A2 (en) | Process for preparation and application of emulgeator and mixture of it appliea in a wide range of ph | |
| HUP1000616A2 (en) | Process for preparation of rosuvastatin salt |