KR890013183A - 선택된 유전자를 박테리아의 염색체상에 통합시키는 방법 및 그 방법에 따라 얻어진 박테리아 - Google Patents
선택된 유전자를 박테리아의 염색체상에 통합시키는 방법 및 그 방법에 따라 얻어진 박테리아 Download PDFInfo
- Publication number
- KR890013183A KR890013183A KR1019890002105A KR890002105A KR890013183A KR 890013183 A KR890013183 A KR 890013183A KR 1019890002105 A KR1019890002105 A KR 1019890002105A KR 890002105 A KR890002105 A KR 890002105A KR 890013183 A KR890013183 A KR 890013183A
- Authority
- KR
- South Korea
- Prior art keywords
- transposon
- gene
- bacterium
- dna sequence
- chromosome
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/847—Erwinia
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/848—Escherichia
- Y10S435/849—Escherichia coli
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
내용 없음.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 넓은 숙주범위를 갖는 Mud II 1734 트란스포존(transposon)의 제한지도를 나타내는 도면;
제2도는 PBR 322(Mud II 1681) 및 PPR 30 플라스미드의 조립 및 제한지도를 나타내는 도면;
제3도는 하기 DNA의 전기영동에 뒤이은 블롯교잡에 관한 결과를 나타내는 도면
·트란스포존의 복사물수가 증식되어진 균주로부터 얻은 DNA(레인f).
·170세대후 분리된 다섯 균주로부터 얻은 DNA(레인 a 내지 e)(탐침은 pCE 1134 ; DNA는 EcoRI에 의해 소화시킴).
Claims (28)
- 선택된 유전자 또는 특정 DNA서열을 박테리아의 염색체 또는 에피솜과 같은 DNA서열속에 통합시킴에 있어, a) 상기 선택된 유전자 또는 선택된 DNA서열을 트란스포존의 필수적 부분 밖에서 트란스포존 속에 클로닝시키고, b) 상기 결함 트란스포존을 상기 박테리아의 염색체 또는 에피솜과 같은 DNA서열속에 통합시키는 방법.
- 박테리아의 염색체 또는 에피솜과 같은 DNA서열속에 선택된 유전자 또는 특정 DNA서열의 복사물을 특정수 만큼 통합시킴에 있어, a) 상기 선택된 유전자 또는 선택된 DNA서열을 트란스포존의 필수적 부분 밖에서 트란스포존 속에 클로닝시키고(상기 트란스포존은 결함 트란스포존임), b) 상기 트란스포존을 상기 박테리아의 염색체 또는 에피솜같은 DNA서열속에 통합시키고, c) 상기 결함 트란스포존이 몇회 전위될 수 있도록 보완하고 특정수의 전위 후에는 보완을 정지시키는 방법.
- 제2항에 있어서, 상기 트란스포존이 전위능력이 없는 방법.
- 제2항 또는 제3항에 있어서, 트란스포존이 트란스포즈아제를 갖지 않는 방법.
- 제4항에 있어서, 트란스포존이 그의 트란스포즈아제를 암호화하는 유전자를 갖지않는 방법.
- 제2항 또는 제3항에 있어서, 상기 트란스포존속에서 트란스포즈아제의 발현이 억제되는 방법.
- 제1항 내지 제6항중 어느 한 항에 있어서, 상기 트란스포존이 플라스미드상에 삽입되고 상기 플라스미드를 가지고 형질전환 시킴으로써 상기 트란스포존이 박테리아 속으로 도입되는 방법.
- 제1항 내지 제6항중 어느 한 항에 있어서, 상기 트란스포존이 박테리오파아지속에 들어있고, 상기 파아지를 가지고 형질도입시킴으로써 박테리아속에 상기 트란스포존이 도입되는 방법.
- 제1항 내지 제8항중 어느 한 항에 있어서, 특정수의 전위를 이루기 위해 보완되는 또 다른 선택된 유전자 또는 특정 DNA서열이 클로닝되어 있는 또 다른 결함 트란스포존이 통합되는 방법.
- 제9항에 있어서, 트란스포즈아제를 발현하는 플라스미드로 상기 박테리아를 형질전환시키거나 상기 파아지로 형질도입시킴으로써 트란스포존을 보완하는 방법.
- 제9항에 있어서, 트란스포즈아제의 유전자가 발현되도록 해주는 리프레서 불활성화 이후 트란스포존이 활성화되는 방법.
- 제1항 내지 제11항중 어느 한 항에 있어서, 트란스포존의 안쪽에 있고 상기 박테리아 속에서 상기 유전자의 발현을 위임하는 프로모터의 조절하에 상기 선택된 유전자가 있는 방법.
- 제1항 내지 제12항중 어느 한 항에 있어서, 상기 트란스포존이 Tn 3, Tn 5, Tn 7, Tn 10, Tn 903, TnHoHo, Is 1, Is 10, Is 50, Mud I 및 MudII파아지로 이루어진 군에서 선택되는 방법.
- 제1항 내지 제13항중 어느 한 항에 있어서, 상기 박테리아가 엔테로박테리아세에, 리조비아세에 또는 슈도모나스 과에 속하는 방법.
- 제14항에 있어서, 상기 박테리아가 에쉐리히아 콜리, 시트로박터 프로인디이, 에르위니아 헤르비콜라 또는 에르위니아 크리산테미, 살모넬라 티피뮤리움, 쉬겔라 손네이, 엔테로박터 클로아세, 세라티아 마프셋센스, 아그로박테리움 투메패시엔스, 슈도모나스 푸티다로 이루어진 군에서 선택되는 방법.
- 제1항 내지 제15항중 어느 한 항에 있어서, 상기 트란스포존이 마아커 유전자를 암호화하기도 하는 방법.
- 제16항에 있어서, 상기 마아커 유전자가 상기 박테리아 속에서 발현된 항생제- 내성 유전자인 방법.
- 제9항 내지 제17항중 어느 한 항에 있어서, 마아커 유전자의 발현을 측정함으로써 전위수를 분석하는 방법.
- 제18항에 있어서, 상기 마아커 유전자가 항생제- 내성 유전자일때 항생제에 대한 내성에 의해 마아커 유전자의 발현을 측정하는 방법.
- 제18항에 있어서, 마아커 유전자(lac오페론 같은)가 그 생성물을 착색 마아커에 의해 시각화할 수 있는 유전자일때 치환분의 착색에 의하여 마아커 유전자의 발현을 측정하는 방법.
- 제1항 내지 제20항중 어느 한 항에 있어서, 상기 숙주 박테리아내에서 상기 선택된 유전자 및 그의 활성 프로모터를 트란스포존의 비필수 부분속에서, 트란스포즈아제에 대한 유전자를 갖지않는 Mud파이지 속에 클로닝시키고, 상기 트란스포존을 양립성 숙주 박테리아의 염색체속에 통합시키고, 트란스폰즈아제를 암호화하는 플라스미드로 숙주를 형질전환시키거나 Mu파아지에 의해 감염시킴으로써 상기 트란스포존을 보완하고, 특정수의 전위 이후에는 자연히 복원된 균주(플라스미드 또는 Mu파아지가 없는)를 선택하는 방법.
- 제1항 내지 제21항중 어느 한 항에 따른 방법을 수행함으로써 얻은 박테리아 균주.
- 제1항 내지 제21항중 어느 한 항에 있어서, 상기 선택된 유전자가 L-트레오닌 생합성 경로로부터의 한개 혹은 몇개의 유전자인 방법.
- 제22항에 있어서, L-트레오닌의 생합성 경로로부터의 모든 혹은 몇몇 유전자를 그의 안쪽에 갖고 있는 트란스포존 복사물 몇개를 염색체속에 포함하는, 트레오닌을 다량생산하는 박테리아.
- 제24항에 있어서, E.콜리인 박테리아.
- 제1항 내지 제21항중 어느 한 항에 있어서, 상기 선택된 유전자가 펩티드 또는 단백질을 암호화하는 방법.
- 제22항, 제24항 또는 제25항의 박테리아를 적합한 배지속에서 배양하고 생성물을 회수하는 것으로 구성되는, 선택된 유전자의 생성물을 제조하는 방법.
- 제21항 또는 제26항에 있어서, 적합한 배지속에서 펩티드 또는 단백질을 생산하고 상기 생성물을 회수하는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR8802078 | 1988-02-22 | ||
FR88/02078 | 1988-02-22 | ||
FR8802078A FR2627508B1 (fr) | 1988-02-22 | 1988-02-22 | Procede pour l'integration d'un gene choisi sur le chromosome d'une bacterie et bacterie obtenue par ledit procede |
Publications (2)
Publication Number | Publication Date |
---|---|
KR890013183A true KR890013183A (ko) | 1989-09-21 |
KR930007579B1 KR930007579B1 (ko) | 1993-08-13 |
Family
ID=9363469
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019890002105A KR930007579B1 (ko) | 1988-02-22 | 1989-02-22 | 선택된 유전자를 박테리아의 염색체상에 통합시키는 방법 및 그 방법에 따라 얻어진 박테리아 |
Country Status (11)
Country | Link |
---|---|
US (1) | US5595889A (ko) |
EP (1) | EP0332488B1 (ko) |
JP (1) | JP2944094B2 (ko) |
KR (1) | KR930007579B1 (ko) |
AT (1) | ATE145668T1 (ko) |
AU (1) | AU624353B2 (ko) |
CA (1) | CA1341190C (ko) |
DE (1) | DE68927486T2 (ko) |
DK (1) | DK175477B1 (ko) |
ES (1) | ES2097115T3 (ko) |
FR (1) | FR2627508B1 (ko) |
Families Citing this family (104)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1990004041A1 (en) * | 1988-10-04 | 1990-04-19 | Dna Plant Technology Corporation | Bacterial detection by phage transduction of detectable phenotype |
WO1994011517A1 (en) | 1992-11-10 | 1994-05-26 | Ajinomoto Co., Inc. | Process for producing l-threonine by fermentation |
CN1036850C (zh) * | 1993-01-13 | 1997-12-31 | 味之素株式会社 | 新的细胞表面蛋白质 |
JPH07155184A (ja) | 1993-12-08 | 1995-06-20 | Ajinomoto Co Inc | 発酵法によるl−リジンの製造法 |
ATE256749T1 (de) * | 1995-01-23 | 2004-01-15 | Novozymes As | Dna-integration durch transposition |
EP0756007A3 (en) * | 1995-06-30 | 1997-10-29 | Ajinomoto Kk | Gene-marking process with artificial transposon |
US6489458B2 (en) * | 1997-03-11 | 2002-12-03 | Regents Of The University Of Minnesota | DNA-based transposon system for the introduction of nucleic acid into DNA of a cell |
EP1975236B1 (en) * | 1998-08-04 | 2015-05-06 | Metabolix, Inc. | Polyhydroxyalkanoate production from polyols |
ATE319835T1 (de) * | 1998-08-18 | 2006-03-15 | Metabolix Inc | Transgene polyhydroxyalkanoat produzierende mikroben |
US7160682B2 (en) * | 1998-11-13 | 2007-01-09 | Regents Of The University Of Minnesota | Nucleic acid transfer vector for the introduction of nucleic acid into the DNA of a cell |
FR2791361B1 (fr) * | 1999-03-22 | 2002-12-06 | Aventis Cropscience Sa | Fragment d'acide nucleique comprenant un gene fonctionnel dans magnaporthe et un transposon impala |
CA2396534C (en) | 1999-12-28 | 2012-10-02 | Esbatech Ag | Intrabodies with defined framework that is stable in a reducing environment and applications thereof |
US20020094575A1 (en) * | 2000-09-14 | 2002-07-18 | Pioneer Hi-Bred International, Inc. | Compositions and methods for stable transformation using Mu bacteriophage cleaved donor complex |
EP1266966B1 (en) | 2001-06-12 | 2009-04-29 | Ajinomoto Co., Inc. | Method for producing L-lysine or L-arginine by using methanol assimilating bacterium |
WO2003089618A2 (en) * | 2002-04-22 | 2003-10-30 | Regents Of The University Of Minnesota | Transposon system and methods of use |
DE102004001674B4 (de) | 2003-01-29 | 2019-01-24 | Ajinomoto Co., Inc. | Verfahren zur Herstellung von L-Lysin unter Einsatz von Methanol verwertenden Bakterien |
FI20030561A0 (fi) * | 2003-04-14 | 2003-04-14 | Finnzymes Oy | Menetelmä nukleiinihappojen siirtämiseksi eukaryoottigenomeihin |
US20060026699A1 (en) * | 2004-06-04 | 2006-02-02 | Largaespada David A | Methods and compositions for identification of genomic sequences |
ES2341264T3 (es) | 2004-08-10 | 2010-06-17 | Ajinomoto Co., Inc. | El uso de fosfocetolasa para producir metabolitos utiles. |
BR122019024048B1 (pt) | 2004-12-28 | 2022-05-24 | Ajinomoto Co., Inc | Método para produzir ácido l-glutâmico |
CA2600882C (en) * | 2005-03-16 | 2013-02-26 | Metabolix, Inc. | Chemically inducible expression of biosynthetic pathways |
BRPI0617491B1 (pt) | 2005-10-18 | 2021-04-27 | Ajinomoto Co., Inc | Processo para produção de ácido succínico |
JP2009089603A (ja) | 2006-02-02 | 2009-04-30 | Ajinomoto Co Inc | メタノール資化性細菌を用いたl−リジンの製造法 |
JP2009153382A (ja) | 2006-03-30 | 2009-07-16 | Ajinomoto Co Inc | メタノール資化性細菌を用いたカルボン酸の製造法 |
JP2009165355A (ja) | 2006-04-28 | 2009-07-30 | Ajinomoto Co Inc | L−アミノ酸を生産する微生物及びl−アミノ酸の製造法 |
EP2021458A1 (en) | 2006-05-23 | 2009-02-11 | Ajinomoto Co., Inc. | A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family |
JP2010017082A (ja) | 2006-10-10 | 2010-01-28 | Ajinomoto Co Inc | L−アミノ酸の製造法 |
WO2008090770A1 (ja) | 2007-01-22 | 2008-07-31 | Ajinomoto Co., Inc. | L-アミノ酸を生産する微生物及びl-アミノ酸の製造法 |
JP2010088301A (ja) | 2007-02-01 | 2010-04-22 | Ajinomoto Co Inc | L−アミノ酸の製造法 |
JP2010110217A (ja) | 2007-02-22 | 2010-05-20 | Ajinomoto Co Inc | L−アミノ酸生産菌及びl−アミノ酸の製造法 |
JP2010130899A (ja) | 2007-03-14 | 2010-06-17 | Ajinomoto Co Inc | L−グルタミン酸系アミノ酸生産微生物及びアミノ酸の製造法 |
EP2149607B1 (en) | 2007-04-10 | 2017-12-20 | Ajinomoto Co., Inc. | Method for production of succinic acid |
EP2149608A4 (en) | 2007-04-16 | 2013-02-20 | Ajinomoto Kk | PROCESS FOR PRODUCING AN ORGANIC ACID |
JP5218400B2 (ja) | 2007-04-17 | 2013-06-26 | 味の素株式会社 | カルボキシル基を有する酸性物質の製造法 |
CN101939412B (zh) | 2007-09-04 | 2016-01-20 | 味之素株式会社 | 生产氨基酸的微生物以及氨基酸的生产方法 |
KR20090058077A (ko) * | 2007-12-04 | 2009-06-09 | 충북대학교 산학협력단 | 생물체 정보의 염색체 내 표지 방법 및 그 정보가 표지된생물체 |
JP5644108B2 (ja) | 2007-12-06 | 2014-12-24 | 味の素株式会社 | 有機酸の製造方法 |
JP2011067095A (ja) | 2008-01-10 | 2011-04-07 | Ajinomoto Co Inc | 発酵法による目的物質の製造法 |
JP5526785B2 (ja) | 2008-01-23 | 2014-06-18 | 味の素株式会社 | L−アミノ酸の製造法 |
WO2009104731A1 (ja) | 2008-02-21 | 2009-08-27 | 味の素株式会社 | L-システイン生産菌及びl-システインの製造法 |
WO2009107631A1 (ja) | 2008-02-25 | 2009-09-03 | 味の素株式会社 | 5’-グアニル酸の製造法 |
JP5332237B2 (ja) | 2008-03-06 | 2013-11-06 | 味の素株式会社 | L−システイン生産菌及びl−システインの製造法 |
JP5488467B2 (ja) | 2008-09-05 | 2014-05-14 | 味の素株式会社 | L−アミノ酸生産菌及びl−アミノ酸の製造法 |
JP2012029565A (ja) | 2008-11-27 | 2012-02-16 | Ajinomoto Co Inc | L−アミノ酸の製造法 |
JP5770099B2 (ja) | 2008-12-12 | 2015-08-26 | メタボリックス,インコーポレイテッド | ポリ(5hv)および5炭素化合物を製造するための環境に優しい方法および組成物 |
JP2010142200A (ja) | 2008-12-22 | 2010-07-01 | Ajinomoto Co Inc | L−リジンの製造法 |
WO2010084995A2 (en) | 2009-01-23 | 2010-07-29 | Ajinomoto Co.,Inc. | A method for producing an l-amino acid |
JP5359409B2 (ja) | 2009-03-12 | 2013-12-04 | 味の素株式会社 | L−システイン生産菌及びl−システインの製造法 |
JPWO2011013707A1 (ja) | 2009-07-29 | 2013-01-10 | 味の素株式会社 | L−アミノ酸の製造法 |
JP2012196144A (ja) | 2009-08-03 | 2012-10-18 | Ajinomoto Co Inc | ビブリオ属細菌を用いたl−リジンの製造法 |
JP5636648B2 (ja) | 2009-08-10 | 2014-12-10 | 味の素株式会社 | 5’−グアニル酸の製造法 |
JP2012223091A (ja) | 2009-08-25 | 2012-11-15 | Ajinomoto Co Inc | L−アミノ酸の製造法 |
RU2009136544A (ru) | 2009-10-05 | 2011-04-10 | Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) (RU) | Способ получения l-цистеина с использованием бактерии семейства enterobacteriaceae |
BR112012012915B1 (pt) | 2009-11-30 | 2020-12-01 | Ajinomoto Co., Inc. | método para produção de l-cisteína, l-cistina, um derivado das mesmas, ou uma mistura das mesmas |
RU2460793C2 (ru) | 2010-01-15 | 2012-09-10 | Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) | Способ получения l-аминокислот с использованием бактерий семейства enterobacteriaceae |
JP5932649B2 (ja) | 2010-09-08 | 2016-06-08 | グリーンフェノール開発株式会社 | コリネ型細菌形質転換体及びそれを用いるフェノールの製造方法 |
WO2012036151A1 (ja) | 2010-09-14 | 2012-03-22 | 味の素株式会社 | 含硫アミノ酸生産菌及び含硫アミノ酸の製造法 |
CN103221533B (zh) | 2010-11-10 | 2017-08-22 | 绿色苯酚开发株式会社 | 棒状细菌转化体及使用该转化体的苯酚的制造方法 |
JP2014036576A (ja) | 2010-12-10 | 2014-02-27 | Ajinomoto Co Inc | L−アミノ酸の製造法 |
JP2014087259A (ja) | 2011-02-22 | 2014-05-15 | Ajinomoto Co Inc | L−システイン生産菌及びl−システインの製造法 |
US9234069B2 (en) | 2011-03-09 | 2016-01-12 | Mitsui Chemicals, Inc. | Pentamethylenediisocyanate, method for producing pentamethylenediisocyanate, polyisocyanate composition, polyurethane resin, and polyurea resin |
WO2012137689A1 (ja) | 2011-04-01 | 2012-10-11 | 味の素株式会社 | L-システインの製造法 |
DE102011006716A1 (de) | 2011-04-04 | 2012-10-04 | Evonik Degussa Gmbh | Mikroorganismus und Verfahren zur fermentativen Herstellung einer organisch-chemischen Verbindung |
JP2014131487A (ja) | 2011-04-18 | 2014-07-17 | Ajinomoto Co Inc | L−システインの製造法 |
AU2012333515B2 (en) | 2011-11-02 | 2015-07-16 | Ajinomoto Co., Inc. | Method for secreting and producing proteins |
EP2778228B1 (en) | 2011-11-11 | 2017-05-17 | Ajinomoto Co., Inc. | Method for producing 2-ketoglutaric acid and derivatives thereof by using a bacterium from the genus pantoea or the genus corynebacterium |
MY185322A (en) | 2013-05-13 | 2021-05-04 | Ajinomoto Kk | Method for producing l-amino acid |
JP2016165225A (ja) | 2013-07-09 | 2016-09-15 | 味の素株式会社 | 有用物質の製造方法 |
JP2016192903A (ja) | 2013-09-17 | 2016-11-17 | 味の素株式会社 | 海藻由来バイオマスからのl−アミノ酸の製造方法 |
JP6569530B2 (ja) | 2013-10-02 | 2019-09-04 | 味の素株式会社 | ヘパロサン生産細菌及びヘパロサンの製造法 |
RU2013146377A (ru) | 2013-10-17 | 2015-04-27 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") | СПОСОБ ПОЛУЧЕНИЯ ИЗОПРЕНА С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ РОДА Bacillus |
WO2015060314A1 (ja) | 2013-10-21 | 2015-04-30 | 味の素株式会社 | L-アミノ酸の製造法 |
BR112016008830B1 (pt) | 2013-10-23 | 2023-02-23 | Ajinomoto Co., Inc | Método para produzir uma substância alvo |
EP3101130B1 (en) | 2014-01-31 | 2020-05-06 | Ajinomoto Co., Inc. | Mutant glutamate-cysteine ligase and method for manufacturing gamma-glutamyl-valyl-glycine |
JP2017216881A (ja) | 2014-12-26 | 2017-12-14 | 味の素株式会社 | ジカルボン酸の製造方法 |
EP3368695A2 (en) | 2015-10-27 | 2018-09-05 | Ajinomoto Co., Inc. | Method for producing aldehyde |
JP6623690B2 (ja) | 2015-10-30 | 2019-12-25 | 味の素株式会社 | グルタミン酸系l−アミノ酸の製造法 |
EP3402891A1 (en) | 2016-01-12 | 2018-11-21 | Ajinomoto Co., Inc. | Method for producing benzaldehyde |
JP7021639B2 (ja) | 2016-10-21 | 2022-02-17 | 味の素株式会社 | タンパク質の分泌生産法 |
CN109937258B (zh) | 2016-10-21 | 2023-06-23 | 味之素株式会社 | 蛋白质的分泌产生方法 |
JP7074133B2 (ja) | 2016-10-26 | 2022-05-24 | 味の素株式会社 | 目的物質の製造方法 |
EP3532631A1 (en) | 2016-10-26 | 2019-09-04 | Ajinomoto Co., Inc. | Method for producing l-methionine or metabolites requiring s-adenosylmethionine for synthesis |
EP3532627A1 (en) | 2016-10-26 | 2019-09-04 | Ajinomoto Co., Inc. | Method for producing objective substance |
CN109890970B (zh) | 2016-10-26 | 2023-04-04 | 味之素株式会社 | 生产目标物质的方法 |
WO2018079684A1 (en) | 2016-10-26 | 2018-05-03 | Ajinomoto Co., Inc. | Method for producing objective substance |
WO2018079705A1 (en) | 2016-10-27 | 2018-05-03 | Ajinomoto Co., Inc. | Method for producing aldehyde |
JP7066977B2 (ja) | 2017-04-03 | 2022-05-16 | 味の素株式会社 | L-アミノ酸の製造法 |
US10858676B2 (en) | 2017-05-22 | 2020-12-08 | Ajinomoto Co., Inc. | Method for producing objective substance |
BR112020005799A2 (pt) | 2017-09-25 | 2020-09-24 | Ajinomoto Co., Inc. | métodos para produzir uma proteína alvo e um dissacarídeo. |
US11680279B2 (en) | 2017-11-29 | 2023-06-20 | Ajinomoto Co., Inc. | Method for producing objective substance |
JP7124338B2 (ja) | 2018-02-27 | 2022-08-24 | 味の素株式会社 | 変異型グルタチオン合成酵素、及び、γ-グルタミルバリルグリシンの製造法 |
JP7480707B2 (ja) | 2018-04-06 | 2024-05-10 | 味の素株式会社 | バチルス属細菌の培養液を含有する農園芸用組成物 |
AU2019256065A1 (en) | 2018-04-20 | 2020-11-26 | Ajinomoto Co., Inc. | Method for secretory production of protein |
WO2020027251A1 (en) | 2018-08-03 | 2020-02-06 | Ajinomoto Co., Inc. | Method for producing objective substance |
WO2020071538A1 (en) | 2018-10-05 | 2020-04-09 | Ajinomoto Co., Inc. | Method for producing target substance by bacterial fermentation |
CN112930359A (zh) | 2018-10-25 | 2021-06-08 | 味之素株式会社 | 蛋白质的分泌生产方法 |
EP3960870A4 (en) | 2019-03-29 | 2023-06-07 | Ajinomoto Co., Inc. | PROCESS FOR PRODUCTION OF ALLOLACTOSE |
CN113811524A (zh) | 2019-05-08 | 2021-12-17 | 味之素株式会社 | 香兰素的制造方法 |
JPWO2021085405A1 (ko) | 2019-10-28 | 2021-05-06 | ||
BR112022017430A2 (pt) | 2020-03-04 | 2022-10-18 | Ajinomoto Kk | Método e composição para produzir um alimento, transglutaminase mutante, gene, vetor, e, microrganismo |
WO2022071061A1 (ja) | 2020-09-29 | 2022-04-07 | 味の素株式会社 | 変異型トランスグルタミナーゼ |
CN116583605A (zh) | 2020-10-28 | 2023-08-11 | 味之素株式会社 | L-氨基酸的制造方法 |
AU2022258873A1 (en) | 2021-04-16 | 2023-11-30 | Inbiose N.V. | Fermentative production |
AU2022306337A1 (en) | 2021-07-07 | 2024-02-01 | Ajinomoto Co., Inc. | Method for secretory production of unnatural-amino-acid-containing protein |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS55156591A (en) * | 1979-05-23 | 1980-12-05 | Ajinomoto Co Inc | Method for stabilizing property of bacteria containing plasmid |
DE3380261D1 (en) * | 1982-02-17 | 1989-08-31 | Ici Plc | Vector system |
US4670388A (en) * | 1982-12-30 | 1987-06-02 | Carnegie Institution Of Washington | Method of incorporating DNA into genome of drosophila |
JPS59205983A (ja) * | 1983-04-28 | 1984-11-21 | ジエネツクス・コ−ポレイシヨン | 異種遺伝子を原核微生物で発現させる方法 |
FR2563533B1 (fr) * | 1984-04-27 | 1986-08-22 | Centre Nat Rech Scient | Procede d'amplification de l'expression d'un gene determine chez bacillus subtilis et souches obtenues |
EP0166659B1 (fr) * | 1984-06-26 | 1989-08-30 | Institut National De La Recherche Agronomique (Inra) | Vecteurs de transformation de la levure yarrowia lipolytica, procédé de transformation et levure transformée |
US4713337A (en) * | 1985-01-03 | 1987-12-15 | Massachusetts Institute Of Technology | Method for deletion of a gene from a bacteria |
FR2580233B1 (fr) * | 1985-04-12 | 1988-11-25 | Rhone Alpes Projets Plast | Procede pour rendre une matiere plastique sensible au rayon laser et permettre son marquage au laser et article obtenu notamment pour le marquage des animaux |
EP0200708B1 (en) * | 1985-04-30 | 1992-12-30 | Monsanto Company | Plant-colonizing microorganisms containing the toxin gene of b. thuringiensis as a chromosomal insertion |
WO1988001646A1 (en) * | 1986-08-26 | 1988-03-10 | Allelix Inc. | Universal system for transposon mutagenesis |
-
1988
- 1988-02-22 FR FR8802078A patent/FR2627508B1/fr not_active Expired - Lifetime
-
1989
- 1989-02-20 DE DE68927486T patent/DE68927486T2/de not_active Expired - Lifetime
- 1989-02-20 EP EP89400468A patent/EP0332488B1/fr not_active Expired - Lifetime
- 1989-02-20 ES ES89400468T patent/ES2097115T3/es not_active Expired - Lifetime
- 1989-02-20 AT AT89400468T patent/ATE145668T1/de not_active IP Right Cessation
- 1989-02-21 AU AU30154/89A patent/AU624353B2/en not_active Expired
- 1989-02-21 DK DK198900789A patent/DK175477B1/da not_active IP Right Cessation
- 1989-02-21 CA CA000591622A patent/CA1341190C/fr not_active Expired - Fee Related
- 1989-02-22 JP JP1042913A patent/JP2944094B2/ja not_active Expired - Lifetime
- 1989-02-22 KR KR1019890002105A patent/KR930007579B1/ko not_active IP Right Cessation
-
1994
- 1994-11-17 US US08/341,460 patent/US5595889A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE68927486T2 (de) | 1997-07-03 |
KR930007579B1 (ko) | 1993-08-13 |
EP0332488B1 (fr) | 1996-11-27 |
DE68927486D1 (de) | 1997-01-09 |
AU3015489A (en) | 1989-08-24 |
DK175477B1 (da) | 2004-11-08 |
FR2627508B1 (fr) | 1990-10-05 |
EP0332488A1 (fr) | 1989-09-13 |
JP2944094B2 (ja) | 1999-08-30 |
ATE145668T1 (de) | 1996-12-15 |
US5595889A (en) | 1997-01-21 |
CA1341190C (fr) | 2001-02-27 |
ES2097115T3 (es) | 1997-04-01 |
AU624353B2 (en) | 1992-06-11 |
DK78989A (da) | 1989-08-22 |
FR2627508A1 (fr) | 1989-08-25 |
JPH02109985A (ja) | 1990-04-23 |
DK78989D0 (da) | 1989-02-21 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR890013183A (ko) | 선택된 유전자를 박테리아의 염색체상에 통합시키는 방법 및 그 방법에 따라 얻어진 박테리아 | |
Platko et al. | Mutations affecting the ability of Escherichia coli Lrp to bind DNA, activate transcription, or respond to leucine | |
Urbanowski et al. | The gcvB gene encodes a small untranslated RNA involved in expression of the dipeptide and oligopeptide transport systems in Escherichia coli | |
Li et al. | The replicator of the nopaline-type Ti plasmid pTiC58 is a member of the repABC family and is influenced by the TraR-dependent quorum-sensing regulatory system | |
Sharma et al. | Role of hha and ler in transcriptional regulation of the esp operon of enterohemorrhagic Escherichia coli O157: H7 | |
Silhavy et al. | Uses of lac fusions for the study of biological problems | |
Givskov et al. | Responses to nutrient starvation in Pseudomonas putida KT2442: two-dimensional electrophoretic analysis of starvation-and stress-induced proteins | |
Fecker et al. | Cloning and expression of the fhu genes involved in iron (III)-hydroxamate uptake by Escherichia coli | |
Danese et al. | CpxP, a stress-combative member of the Cpx regulon | |
Hiraga et al. | Chromosome partitioning in Escherichia coli: novel mutants producing anucleate cells | |
Lee et al. | FarRRegulates the farAB-Encoded Efflux Pump of Neisseriagonorrhoeae via an MtrR RegulatoryMechanism | |
Weaver et al. | Identification, characterization, and nucleotide sequence of a region of Enterococcus faecalis pheromone-responsive plasmid pAD1 capable of autonomous replication | |
Smajs et al. | Colicin U, a novel colicin produced by Shigella boydii | |
Dowds et al. | Relationship among oxidative stress, growth cycle, and sporulation in Bacillus subtilis | |
DeFranco et al. | Molecular cloning of chemotaxis genes and overproduction of gene products in the bacterial sensing system | |
Belas et al. | Genetic determinants of Silicibacter sp. TM1040 motility | |
Navazo et al. | Three independent signalling pathways repress motility in Pseudomonas fluorescens F113 | |
Elledge et al. | Phasmid vectors for identification of genes by complementation of Escherichia coli mutants | |
Shiba et al. | Suppressors of the secY24 mutation: identification and characterization of additional ssy genes in Escherichia coli | |
Spitzer et al. | dfp Gene of Escherichia coli K-12, a locus affecting DNA synthesis, codes for a flavoprotein | |
Schmid | Structure and function of the bacterial chromosome | |
Pierce et al. | Mutations in DivL and CckA rescue a divJ null mutant of Caulobacter crescentus by reducing the activity of CtrA | |
Gomes et al. | Heat shock protein synthesis during development in Caulobacter crescentus | |
Obuchowski et al. | Stability of CII is a key element in the cold stress response of bacteriophage lambda infection | |
Jakowec et al. | Mutational analysis of the open reading frames in the transposable element IS1. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A201 | Request for examination | ||
E902 | Notification of reason for refusal | ||
E902 | Notification of reason for refusal | ||
G160 | Decision to publish patent application | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant | ||
FPAY | Annual fee payment |
Payment date: 20080718 Year of fee payment: 16 |
|
EXPY | Expiration of term |