ATE256749T1 - Dna-integration durch transposition - Google Patents
Dna-integration durch transpositionInfo
- Publication number
- ATE256749T1 ATE256749T1 AT96900546T AT96900546T ATE256749T1 AT E256749 T1 ATE256749 T1 AT E256749T1 AT 96900546 T AT96900546 T AT 96900546T AT 96900546 T AT96900546 T AT 96900546T AT E256749 T1 ATE256749 T1 AT E256749T1
- Authority
- AT
- Austria
- Prior art keywords
- pct
- transposition
- marker
- sec
- interest
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
- Superconductors And Manufacturing Methods Therefor (AREA)
- Amplifiers (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK8395 | 1995-01-23 | ||
DK79995 | 1995-07-06 | ||
PCT/DK1996/000038 WO1996023073A1 (en) | 1995-01-23 | 1996-01-23 | Dna integration by transposition |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE256749T1 true ATE256749T1 (de) | 2004-01-15 |
Family
ID=26063271
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT96900546T ATE256749T1 (de) | 1995-01-23 | 1996-01-23 | Dna-integration durch transposition |
Country Status (9)
Country | Link |
---|---|
US (1) | US5882888A (de) |
EP (1) | EP0805867B1 (de) |
JP (1) | JPH10512450A (de) |
CN (1) | CN1177380A (de) |
AT (1) | ATE256749T1 (de) |
AU (1) | AU4431196A (de) |
DE (1) | DE69631118T2 (de) |
DK (1) | DK0805867T3 (de) |
WO (1) | WO1996023073A1 (de) |
Families Citing this family (74)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6570001B1 (en) * | 1997-06-20 | 2003-05-27 | Institut Pasteur | Polynucleotides and their use for detecting resistance to streptogramin A or to streptogramin B and related compounds |
US5955310A (en) * | 1998-02-26 | 1999-09-21 | Novo Nordisk Biotech, Inc. | Methods for producing a polypeptide in a bacillus cell |
HU228700B1 (en) | 1998-07-22 | 2013-05-28 | Stichting Dienst Landbouwkundi | Streptococcus suis vaccines and diagnostic tests |
US6890726B1 (en) | 1999-04-06 | 2005-05-10 | Oklahoma Medical Research Foundation | Method for selecting recombinase variants with altered specificity |
EP1297170B1 (de) * | 2000-06-23 | 2016-03-02 | Novozymes A/S | Verfahren zur stabilen chromosomalen integration von mehreren kopien von genen |
AU2001279254A1 (en) * | 2000-08-11 | 2002-02-25 | Genencor International, Inc. | Bacillus transformation, transformants and mutant libraries |
US20020132350A1 (en) * | 2000-09-14 | 2002-09-19 | Pioneer Hi-Bred International, Inc. | Targeted genetic manipulation using Mu bacteriophage cleaved donor complex |
JP4275413B2 (ja) * | 2001-02-02 | 2009-06-10 | イーデー−レーリスタット,インスティチュート フォール ディールハウデレイ エン ディールゲゾントヘイト ベー.フェー. | ストレプトコッカス・スイスの環境的に調節された遺伝子 |
DE60321901D1 (de) | 2002-04-10 | 2008-08-14 | Novozymes As | Verbesserte bacillus-wirtszelle |
US20040224340A1 (en) * | 2003-04-21 | 2004-11-11 | Filutowicz Marcin S. | Displacing a plasmid in a bacterial population |
US20060236414A1 (en) | 2003-06-19 | 2006-10-19 | Novozymes A/S | Proteases and methods for producing them |
EP1766002B1 (de) | 2004-06-21 | 2015-11-18 | Novozymes A/S | Mehrere kopien wenigstens zweier offener leserrahmen stabil erhalten in gleicher orientierung |
DE102005036449A1 (de) * | 2005-08-03 | 2007-02-08 | Robert Bosch Gmbh | Elektrogerät, insbesondere Elektrohandwerkzeug |
WO2007138049A2 (en) | 2006-05-31 | 2007-12-06 | Novozymes A/S | Chloramphenicol resistance selection in bacillus licheniformis |
DE102007021001A1 (de) | 2007-05-04 | 2008-11-06 | Ab Enzymes Gmbh | Expressionssystem zur antibiotikafreien Produktion von Polypeptiden |
JP2011067095A (ja) | 2008-01-10 | 2011-04-07 | Ajinomoto Co Inc | 発酵法による目的物質の製造法 |
WO2010083841A2 (en) * | 2009-01-23 | 2010-07-29 | Nsgene A/S | Improved cell lines and their use in encapsulated cell biodelivery |
JP2014131487A (ja) | 2011-04-18 | 2014-07-17 | Ajinomoto Co Inc | L−システインの製造法 |
DK2758074T3 (da) | 2011-09-23 | 2020-07-27 | Univ Loma Linda | Bakteriestammer der udtrykker methylasegener og anvendelser deraf |
AU2012333515B2 (en) | 2011-11-02 | 2015-07-16 | Ajinomoto Co., Inc. | Method for secreting and producing proteins |
EP2778228B1 (de) | 2011-11-11 | 2017-05-17 | Ajinomoto Co., Inc. | Verfahren zur herstellung von 2-ketoglutarsäure und derivate davon unter verwendung eines bakteriums der gattung pantoea oder der gattung corynebacterium |
WO2013173711A1 (en) | 2012-05-18 | 2013-11-21 | Novozymes A/S | Bacterial mutants with improved transformation efficiency |
WO2013178699A1 (en) | 2012-05-31 | 2013-12-05 | Novozymes A/S | Isopropanol production by bacterial hosts |
MY185322A (en) | 2013-05-13 | 2021-05-04 | Ajinomoto Kk | Method for producing l-amino acid |
JP2016165225A (ja) | 2013-07-09 | 2016-09-15 | 味の素株式会社 | 有用物質の製造方法 |
JP2016192903A (ja) | 2013-09-17 | 2016-11-17 | 味の素株式会社 | 海藻由来バイオマスからのl−アミノ酸の製造方法 |
JP6569530B2 (ja) | 2013-10-02 | 2019-09-04 | 味の素株式会社 | ヘパロサン生産細菌及びヘパロサンの製造法 |
WO2015060314A1 (ja) | 2013-10-21 | 2015-04-30 | 味の素株式会社 | L-アミノ酸の製造法 |
BR112016008830B1 (pt) | 2013-10-23 | 2023-02-23 | Ajinomoto Co., Inc | Método para produzir uma substância alvo |
EP3101130B1 (de) | 2014-01-31 | 2020-05-06 | Ajinomoto Co., Inc. | Mutante glutamat-cystein-ligase und verfahren zur herstellung von gamma-glutamyl-valyl-glycin |
JP2017216881A (ja) | 2014-12-26 | 2017-12-14 | 味の素株式会社 | ジカルボン酸の製造方法 |
JP6611305B2 (ja) * | 2015-03-31 | 2019-11-27 | 学校法人神奈川大学 | 除去標的dnaの除去方法、dnaカセット、及び発現ベクター |
CN107624134A (zh) | 2015-05-12 | 2018-01-23 | 诺维信公司 | 缺失羊毛硫抗生素基因的地衣芽孢杆菌宿主细胞 |
EP3368695A2 (de) | 2015-10-27 | 2018-09-05 | Ajinomoto Co., Inc. | Verfahren zur herstellung von aldehyd |
JP6623690B2 (ja) | 2015-10-30 | 2019-12-25 | 味の素株式会社 | グルタミン酸系l−アミノ酸の製造法 |
EP3402891A1 (de) | 2016-01-12 | 2018-11-21 | Ajinomoto Co., Inc. | Verfahren zur herstellung von benzaldehyd |
CN109937258B (zh) | 2016-10-21 | 2023-06-23 | 味之素株式会社 | 蛋白质的分泌产生方法 |
JP7021639B2 (ja) | 2016-10-21 | 2022-02-17 | 味の素株式会社 | タンパク質の分泌生産法 |
CN109804072A (zh) | 2016-10-25 | 2019-05-24 | 诺维信公司 | 地衣芽孢杆菌中flp介导的基因组整合 |
EP3532631A1 (de) | 2016-10-26 | 2019-09-04 | Ajinomoto Co., Inc. | Verfahren zur herstellung von l-methionin oder metaboliten, die s-adenosylmethionin zur synthese benötigen |
JP7074133B2 (ja) | 2016-10-26 | 2022-05-24 | 味の素株式会社 | 目的物質の製造方法 |
EP3532627A1 (de) | 2016-10-26 | 2019-09-04 | Ajinomoto Co., Inc. | Verfahren zur herstellung einer zielsubstanz |
WO2018079684A1 (en) | 2016-10-26 | 2018-05-03 | Ajinomoto Co., Inc. | Method for producing objective substance |
CN109890970B (zh) | 2016-10-26 | 2023-04-04 | 味之素株式会社 | 生产目标物质的方法 |
WO2018079705A1 (en) | 2016-10-27 | 2018-05-03 | Ajinomoto Co., Inc. | Method for producing aldehyde |
WO2018134386A1 (en) | 2017-01-23 | 2018-07-26 | Novozymes A/S | Host cells and methods for producing double-stranded rna |
JP7066977B2 (ja) | 2017-04-03 | 2022-05-16 | 味の素株式会社 | L-アミノ酸の製造法 |
CN108728477B (zh) * | 2017-04-24 | 2022-02-22 | 华东理工大学 | 一种高效的转座突变系统及构建方法 |
US10858676B2 (en) | 2017-05-22 | 2020-12-08 | Ajinomoto Co., Inc. | Method for producing objective substance |
BR112020005799A2 (pt) | 2017-09-25 | 2020-09-24 | Ajinomoto Co., Inc. | métodos para produzir uma proteína alvo e um dissacarídeo. |
EP3707252A1 (de) | 2017-11-10 | 2020-09-16 | Novozymes A/S | Temperaturempfindliches cas9-protein |
US11680279B2 (en) | 2017-11-29 | 2023-06-20 | Ajinomoto Co., Inc. | Method for producing objective substance |
US20210017544A1 (en) | 2017-12-22 | 2021-01-21 | Novozymes A/S | Counter-Selection by Inhibition of Conditionally Essential Genes |
JP7124338B2 (ja) | 2018-02-27 | 2022-08-24 | 味の素株式会社 | 変異型グルタチオン合成酵素、及び、γ-グルタミルバリルグリシンの製造法 |
JP7480707B2 (ja) | 2018-04-06 | 2024-05-10 | 味の素株式会社 | バチルス属細菌の培養液を含有する農園芸用組成物 |
AU2019256065A1 (en) | 2018-04-20 | 2020-11-26 | Ajinomoto Co., Inc. | Method for secretory production of protein |
WO2020027251A1 (en) | 2018-08-03 | 2020-02-06 | Ajinomoto Co., Inc. | Method for producing objective substance |
CN112930359A (zh) | 2018-10-25 | 2021-06-08 | 味之素株式会社 | 蛋白质的分泌生产方法 |
US20220112478A1 (en) | 2019-01-30 | 2022-04-14 | Novozymes A/S | Cognate Foldase Co-Expression |
CN113785056A (zh) | 2019-03-08 | 2021-12-10 | 诺维信公司 | 改善蛋白酶表达的手段和方法 |
EP3960870A4 (de) | 2019-03-29 | 2023-06-07 | Ajinomoto Co., Inc. | Verfahren zur herstellung von allolactose |
CN113811524A (zh) | 2019-05-08 | 2021-12-17 | 味之素株式会社 | 香兰素的制造方法 |
EP3969576A1 (de) | 2019-05-15 | 2022-03-23 | Novozymes A/S | Temperaturempfindliche rna-geführte endonuklease |
WO2020260061A1 (en) | 2019-06-25 | 2020-12-30 | Novozymes A/S | Counter-selection by inhibition of conditionally essential genes |
JPWO2021085405A1 (de) | 2019-10-28 | 2021-05-06 | ||
BR112022017430A2 (pt) | 2020-03-04 | 2022-10-18 | Ajinomoto Kk | Método e composição para produzir um alimento, transglutaminase mutante, gene, vetor, e, microrganismo |
CN115698301A (zh) * | 2020-03-30 | 2023-02-03 | 中国科学院动物研究所 | 活性dna转座子系统及其使用方法 |
WO2022071061A1 (ja) | 2020-09-29 | 2022-04-07 | 味の素株式会社 | 変異型トランスグルタミナーゼ |
CN116583605A (zh) | 2020-10-28 | 2023-08-11 | 味之素株式会社 | L-氨基酸的制造方法 |
AU2022306337A1 (en) | 2021-07-07 | 2024-02-01 | Ajinomoto Co., Inc. | Method for secretory production of unnatural-amino-acid-containing protein |
EP4370534A1 (de) | 2021-07-13 | 2024-05-22 | Novozymes A/S | Rekombinante cutinase-expression |
WO2023104846A1 (en) | 2021-12-10 | 2023-06-15 | Novozymes A/S | Improved protein production in recombinant bacteria |
WO2023247514A1 (en) | 2022-06-22 | 2023-12-28 | Novozymes A/S | Recombinant mannanase expression |
US20240117393A1 (en) | 2022-09-30 | 2024-04-11 | Ajinomoto Co., Inc. | Method for producing l-amino acid |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2627508B1 (fr) * | 1988-02-22 | 1990-10-05 | Eurolysine | Procede pour l'integration d'un gene choisi sur le chromosome d'une bacterie et bacterie obtenue par ledit procede |
US5102797A (en) * | 1989-05-26 | 1992-04-07 | Dna Plant Technology Corporation | Introduction of heterologous genes into bacteria using transposon flanked expression cassette and a binary vector system |
US5527695A (en) * | 1993-01-29 | 1996-06-18 | Purdue Research Foundation | Controlled modification of eukaryotic genomes |
US5843744A (en) * | 1993-07-08 | 1998-12-01 | Ecogen Inc. | Bacillus thuringiensis Tn5401 proteins |
-
1996
- 1996-01-23 AT AT96900546T patent/ATE256749T1/de not_active IP Right Cessation
- 1996-01-23 DK DK96900546T patent/DK0805867T3/da active
- 1996-01-23 AU AU44311/96A patent/AU4431196A/en not_active Abandoned
- 1996-01-23 EP EP96900546A patent/EP0805867B1/de not_active Expired - Lifetime
- 1996-01-23 JP JP8522557A patent/JPH10512450A/ja active Pending
- 1996-01-23 WO PCT/DK1996/000038 patent/WO1996023073A1/en active IP Right Grant
- 1996-01-23 DE DE69631118T patent/DE69631118T2/de not_active Expired - Lifetime
- 1996-01-23 CN CN96192093A patent/CN1177380A/zh active Pending
- 1996-01-23 US US08/875,154 patent/US5882888A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE69631118D1 (de) | 2004-01-29 |
EP0805867A1 (de) | 1997-11-12 |
WO1996023073A1 (en) | 1996-08-01 |
EP0805867B1 (de) | 2003-12-17 |
US5882888A (en) | 1999-03-16 |
JPH10512450A (ja) | 1998-12-02 |
CN1177380A (zh) | 1998-03-25 |
AU4431196A (en) | 1996-08-14 |
DK0805867T3 (da) | 2004-04-13 |
DE69631118T2 (de) | 2004-07-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE69631118D1 (de) | Dna-integration durch transposition | |
Smalla et al. | Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity of IncQ-like plasmids | |
Choy et al. | MorA defines a new class of regulators affecting flagellar development and biofilm formation in diverse Pseudomonas species | |
Thiel et al. | A second nitrogenase in vegetative cells of a heterocyst-forming cyanobacterium. | |
Chen et al. | Genetic characterization of soybean rhizobia in Paraguay | |
Heuer et al. | Spreading antibiotic resistance through spread manure: characteristics of a novel plasmid type with low% G+ C content | |
Faelen et al. | Bacteriophage Mu-1: a tool to transpose and to localize bacterial genes | |
Buikema et al. | Molecular genetics of cyanobacterial development | |
Fall et al. | Differences between bacterial communities in the gut of a soil-feeding termite (Cubitermes niokoloensis) and its mounds | |
Gupta et al. | A new Bacteroides conjugative transposon that carries an ermB gene | |
Ike et al. | Efficient transfer of the pheromone-independent Enterococcus faecium plasmid pMG1 (Gmr)(65.1 kilobases) to Enterococcus strains during broth mating | |
Wei et al. | A mobile quorum-sensing system in Serratia marcescens | |
Baumann et al. | The genetic properties of the primary endosymbionts of mealybugs differ from those of other endosymbionts of plant sap-sucking insects | |
ATE239036T1 (de) | Rekombinantes core-streptavidin | |
Fels et al. | Rapid transposon liquid enrichment sequencing (TnLE-seq) for gene fitness evaluation in underdeveloped bacterial systems | |
Akman et al. | Genome size determination and coding capacity of Sodalis glossinidius, an enteric symbiont of tsetse flies, as revealed by hybridization to Escherichia coli gene arrays | |
Krishnavajhala et al. | Imaging of Borrelia turicatae producing the green fluorescent protein reveals persistent colonization of the Ornithodoros turicata midgut and salivary glands from nymphal acquisition through transmission | |
Tan et al. | Diverse phenotypes resulting from polyphosphate kinase gene (ppk1) inactivation in different strains of Helicobacter pylori | |
ATE44766T1 (de) | Verfahren zur vergroesserung der expression eines bestimmten gens im bacillus subtilis und erhaltene staemme. | |
Vedantam et al. | Bacteroides fragilis transfer factor Tn 5520: the smallest bacterial mobilizable transposon containing single integrase and mobilization genes that function in Escherichia coli | |
Bordenstein et al. | The most widespread phage in animals: Genomics and taxonomic classification of Phage WO | |
Gladney et al. | Molecular and phenotypic characterization of Vibrio navarrensis isolates associated with human illness | |
Brooks et al. | TfoX-based genetic mapping identifies Vibrio fischeri strain-level differences and reveals a common lineage of laboratory strains | |
White et al. | Extensive genome rearrangements and multiple horizontal gene transfers in a population of Pyrococcus isolates from Vulcano Island, Italy | |
Shiojima et al. | High-level plasmid-mediated gentamicin resistance and pheromone response of plasmids present in clinical isolates of Enterococcus faecalis |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |