FI20030561A0 - Menetelmä nukleiinihappojen siirtämiseksi eukaryoottigenomeihin - Google Patents

Menetelmä nukleiinihappojen siirtämiseksi eukaryoottigenomeihin

Info

Publication number
FI20030561A0
FI20030561A0 FI20030561A FI20030561A FI20030561A0 FI 20030561 A0 FI20030561 A0 FI 20030561A0 FI 20030561 A FI20030561 A FI 20030561A FI 20030561 A FI20030561 A FI 20030561A FI 20030561 A0 FI20030561 A0 FI 20030561A0
Authority
FI
Finland
Prior art keywords
transposition
nucleic acids
kit
eukaryotic genomes
transferring nucleic
Prior art date
Application number
FI20030561A
Other languages
English (en)
Swedish (sv)
Inventor
Harri Savilahti
Mikko Frilander
Xiaojuan Meng
Anja Paatero
Maria Pajunen
Hilkka Turakainen
Original Assignee
Finnzymes Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Finnzymes Oy filed Critical Finnzymes Oy
Priority to FI20030561A priority Critical patent/FI20030561A0/fi
Publication of FI20030561A0 publication Critical patent/FI20030561A0/fi
Priority to US10/553,353 priority patent/US8026052B1/en
Priority to EP04727306A priority patent/EP1613758B1/en
Priority to CA2522438A priority patent/CA2522438C/en
Priority to PCT/FI2004/000228 priority patent/WO2004090146A1/en
Priority to JP2006505640A priority patent/JP4809213B2/ja
Priority to DE602004029875T priority patent/DE602004029875D1/de
Priority to AT04727306T priority patent/ATE486947T1/de
Priority to US13/229,258 priority patent/US8192934B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
FI20030561A 2003-04-14 2003-04-14 Menetelmä nukleiinihappojen siirtämiseksi eukaryoottigenomeihin FI20030561A0 (fi)

Priority Applications (9)

Application Number Priority Date Filing Date Title
FI20030561A FI20030561A0 (fi) 2003-04-14 2003-04-14 Menetelmä nukleiinihappojen siirtämiseksi eukaryoottigenomeihin
US10/553,353 US8026052B1 (en) 2003-04-14 2004-04-14 Delivery of nucleic acids into eukaryotic genomes using in vitro assembled Mu transposition complexes
EP04727306A EP1613758B1 (en) 2003-04-14 2004-04-14 Delivery of nucleic acids into eukaryotic genomes using in vitro assembled mu transposition complexes
CA2522438A CA2522438C (en) 2003-04-14 2004-04-14 Delivery of nucleic acids into eukaryotic genomes using in vitro assembled mu transposition complexes
PCT/FI2004/000228 WO2004090146A1 (en) 2003-04-14 2004-04-14 Delivery of nucleic acids into eukaryotic genomes using in vitro assembled mu transposition complexes
JP2006505640A JP4809213B2 (ja) 2003-04-14 2004-04-14 Ｉｎｖｉｔｒｏで構築されたＭｕ転位複合体を用いて真核生物ゲノムに核酸を送達する方法
DE602004029875T DE602004029875D1 (de) 2003-04-14 2004-04-14 Zuführung von nukleinsäuren in eukaryontische genome unter verwendung in vitro zusammengesetzter mu-transpositionskomplexe
AT04727306T ATE486947T1 (de) 2003-04-14 2004-04-14 Zuführung von nukleinsäuren in eukaryontische genome unter verwendung in vitro zusammengesetzter mu-transpositionskomplexe
US13/229,258 US8192934B2 (en) 2003-04-14 2011-09-09 Method for delivering nucleic acid into eukaryotic genomes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FI20030561A FI20030561A0 (fi) 2003-04-14 2003-04-14 Menetelmä nukleiinihappojen siirtämiseksi eukaryoottigenomeihin

Publications (1)

Publication Number Publication Date
FI20030561A0 true FI20030561A0 (fi) 2003-04-14

Family

ID=8565964

Family Applications (1)

Application Number Title Priority Date Filing Date
FI20030561A FI20030561A0 (fi) 2003-04-14 2003-04-14 Menetelmä nukleiinihappojen siirtämiseksi eukaryoottigenomeihin

Country Status (8)

Country Link
US (2) US8026052B1 (fi)
EP (1) EP1613758B1 (fi)
JP (1) JP4809213B2 (fi)
AT (1) ATE486947T1 (fi)
CA (1) CA2522438C (fi)
DE (1) DE602004029875D1 (fi)
FI (1) FI20030561A0 (fi)
WO (1) WO2004090146A1 (fi)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI20030561A0 (fi) * 2003-04-14 2003-04-14 Finnzymes Oy Menetelmä nukleiinihappojen siirtämiseksi eukaryoottigenomeihin
FI20075520A0 (fi) * 2007-07-06 2007-07-06 Finnzymes Oy Nukleiinihappojen siirtäminen ihmisen katansolujen genomeihin käyttäen in vitro -koottuja Mu-transpositiokomplekseja
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
AU2014277301B2 (en) 2013-06-04 2016-10-13 Nordic Aquafarms Europe As A container, series of containers and method for treating liquids
WO2017189894A1 (en) * 2016-04-27 2017-11-02 Yale University Multiplex genome engineering in eukaryotes
IL292003A (en) * 2019-10-10 2022-06-01 Carlsberg As A method for preparing mutant plants

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2627508B1 (fr) * 1988-02-22 1990-10-05 Eurolysine Procede pour l'integration d'un gene choisi sur le chromosome d'une bacterie et bacterie obtenue par ledit procede
WO1995001095A1 (en) * 1993-06-30 1995-01-12 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Transformed eukaryotic cells, and transposon-based transformation vectors
US5677170A (en) * 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
US6159736A (en) * 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
GB9917531D0 (en) * 1999-07-26 1999-09-29 Arrow Therapeutics Ltd Transposon mediated differential hybridisation
JP3451311B2 (ja) * 2000-02-28 2003-09-29 独立行政法人産業技術総合研究所 染色体組込み用ｄｎａ断片、ベクター及びそれらを用いた染色体への遺伝子導入方法
US20020132350A1 (en) * 2000-09-14 2002-09-19 Pioneer Hi-Bred International, Inc. Targeted genetic manipulation using Mu bacteriophage cleaved donor complex
US20020094575A1 (en) * 2000-09-14 2002-07-18 Pioneer Hi-Bred International, Inc. Compositions and methods for stable transformation using Mu bacteriophage cleaved donor complex
US20030143740A1 (en) 2001-10-15 2003-07-31 Christine Wooddell Processes for transposase mediated integration into mammalian cells
AU2002366950A1 (en) * 2001-12-11 2003-07-09 Van Andel Institute Generation of transgenic mice by transgene-mediated rescue of spermatogenesis
FI20030561A0 (fi) * 2003-04-14 2003-04-14 Finnzymes Oy Menetelmä nukleiinihappojen siirtämiseksi eukaryoottigenomeihin

Also Published As

Publication number Publication date
JP2006522596A (ja) 2006-10-05
ATE486947T1 (de) 2010-11-15
CA2522438A1 (en) 2004-10-21
US8026052B1 (en) 2011-09-27
CA2522438C (en) 2012-09-25
EP1613758A1 (en) 2006-01-11
DE602004029875D1 (de) 2010-12-16
JP4809213B2 (ja) 2011-11-09
US8192934B2 (en) 2012-06-05
EP1613758B1 (en) 2010-11-03
WO2004090146A1 (en) 2004-10-21
US20120015831A1 (en) 2012-01-19

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