FI20075520A0 - Nukleiinihappojen siirtäminen ihmisen katansolujen genomeihin käyttäen in vitro -koottuja Mu-transpositiokomplekseja - Google Patents

Nukleiinihappojen siirtäminen ihmisen katansolujen genomeihin käyttäen in vitro -koottuja Mu-transpositiokomplekseja

Info

Publication number
FI20075520A0
FI20075520A0 FI20075520A FI20075520A FI20075520A0 FI 20075520 A0 FI20075520 A0 FI 20075520A0 FI 20075520 A FI20075520 A FI 20075520A FI 20075520 A FI20075520 A FI 20075520A FI 20075520 A0 FI20075520 A0 FI 20075520A0
Authority
FI
Finland
Prior art keywords
introduction
nucleic acids
stem cell
human stem
cell genomes
Prior art date
Application number
FI20075520A
Other languages
English (en)
Swedish (sv)
Inventor
Harri Savilahti
Original Assignee
Finnzymes Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Finnzymes Oy filed Critical Finnzymes Oy
Priority to FI20075520A priority Critical patent/FI20075520A0/fi
Publication of FI20075520A0 publication Critical patent/FI20075520A0/fi
Priority to EP08775536A priority patent/EP2171069A4/en
Priority to PCT/FI2008/050411 priority patent/WO2009007503A1/en
Priority to US12/667,853 priority patent/US20100173800A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10111—Myoviridae
    • C12N2795/10141—Use of virus, viral particle or viral elements as a vector
    • C12N2795/10143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
FI20075520A 2007-07-06 2007-07-06 Nukleiinihappojen siirtäminen ihmisen katansolujen genomeihin käyttäen in vitro -koottuja Mu-transpositiokomplekseja FI20075520A0 (fi)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FI20075520A FI20075520A0 (fi) 2007-07-06 2007-07-06 Nukleiinihappojen siirtäminen ihmisen katansolujen genomeihin käyttäen in vitro -koottuja Mu-transpositiokomplekseja
EP08775536A EP2171069A4 (en) 2007-07-06 2008-07-04 INTRODUCTION OF NUCLEIC ACIDS IN HUMAN STEM CELL GENOMES USING IN VITRO ASSEMBLED MU TRANSPOSITION COMPLEXES
PCT/FI2008/050411 WO2009007503A1 (en) 2007-07-06 2008-07-04 Delivery of nucleic acids into genomes of human stem cells using in vitro assembled mu transposition complexes
US12/667,853 US20100173800A1 (en) 2007-07-06 2008-07-04 Delivery of nucleic acids into genomes of human stem cells using in vitro assembled mu transposition complexes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FI20075520A FI20075520A0 (fi) 2007-07-06 2007-07-06 Nukleiinihappojen siirtäminen ihmisen katansolujen genomeihin käyttäen in vitro -koottuja Mu-transpositiokomplekseja

Publications (1)

Publication Number Publication Date
FI20075520A0 true FI20075520A0 (fi) 2007-07-06

Family

ID=38331608

Family Applications (1)

Application Number Title Priority Date Filing Date
FI20075520A FI20075520A0 (fi) 2007-07-06 2007-07-06 Nukleiinihappojen siirtäminen ihmisen katansolujen genomeihin käyttäen in vitro -koottuja Mu-transpositiokomplekseja

Country Status (4)

Country Link
US (1) US20100173800A1 (fi)
EP (1) EP2171069A4 (fi)
FI (1) FI20075520A0 (fi)
WO (1) WO2009007503A1 (fi)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021041922A1 (en) * 2019-08-30 2021-03-04 The Broad Institute, Inc. Crispr-associated mu transposase systems

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0885307A1 (en) * 1996-02-09 1998-12-23 Vereniging Het Nederlands Kanker Instituut Vectors and methods for providing cells with additional nucleic acid material integrated in the genome of said cells
US20020132350A1 (en) * 2000-09-14 2002-09-19 Pioneer Hi-Bred International, Inc. Targeted genetic manipulation using Mu bacteriophage cleaved donor complex
FI20030561A0 (fi) * 2003-04-14 2003-04-14 Finnzymes Oy Menetelmä nukleiinihappojen siirtämiseksi eukaryoottigenomeihin

Also Published As

Publication number Publication date
EP2171069A4 (en) 2011-01-05
US20100173800A1 (en) 2010-07-08
WO2009007503A1 (en) 2009-01-15
EP2171069A1 (en) 2010-04-07

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