FI20075520A0 - Introduction of nucleic acids into human stem cell genomes using in vitro collected Mu transposition complexes - Google Patents

Introduction of nucleic acids into human stem cell genomes using in vitro collected Mu transposition complexes

Info

Publication number
FI20075520A0
FI20075520A0 FI20075520A FI20075520A FI20075520A0 FI 20075520 A0 FI20075520 A0 FI 20075520A0 FI 20075520 A FI20075520 A FI 20075520A FI 20075520 A FI20075520 A FI 20075520A FI 20075520 A0 FI20075520 A0 FI 20075520A0
Authority
FI
Finland
Prior art keywords
introduction
nucleic acids
stem cell
human stem
cell genomes
Prior art date
Application number
FI20075520A
Other languages
Finnish (fi)
Swedish (sv)
Inventor
Harri Savilahti
Original Assignee
Finnzymes Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Finnzymes Oy filed Critical Finnzymes Oy
Priority to FI20075520A priority Critical patent/FI20075520A0/en
Publication of FI20075520A0 publication Critical patent/FI20075520A0/en
Priority to EP08775536A priority patent/EP2171069A4/en
Priority to US12/667,853 priority patent/US20100173800A1/en
Priority to PCT/FI2008/050411 priority patent/WO2009007503A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10111—Myoviridae
    • C12N2795/10141—Use of virus, viral particle or viral elements as a vector
    • C12N2795/10143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
FI20075520A 2007-07-06 2007-07-06 Introduction of nucleic acids into human stem cell genomes using in vitro collected Mu transposition complexes FI20075520A0 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FI20075520A FI20075520A0 (en) 2007-07-06 2007-07-06 Introduction of nucleic acids into human stem cell genomes using in vitro collected Mu transposition complexes
EP08775536A EP2171069A4 (en) 2007-07-06 2008-07-04 Delivery of nucleic acids into genomes of human stem cells using in vitro assembled mu transposition complexes
US12/667,853 US20100173800A1 (en) 2007-07-06 2008-07-04 Delivery of nucleic acids into genomes of human stem cells using in vitro assembled mu transposition complexes
PCT/FI2008/050411 WO2009007503A1 (en) 2007-07-06 2008-07-04 Delivery of nucleic acids into genomes of human stem cells using in vitro assembled mu transposition complexes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FI20075520A FI20075520A0 (en) 2007-07-06 2007-07-06 Introduction of nucleic acids into human stem cell genomes using in vitro collected Mu transposition complexes

Publications (1)

Publication Number Publication Date
FI20075520A0 true FI20075520A0 (en) 2007-07-06

Family

ID=38331608

Family Applications (1)

Application Number Title Priority Date Filing Date
FI20075520A FI20075520A0 (en) 2007-07-06 2007-07-06 Introduction of nucleic acids into human stem cell genomes using in vitro collected Mu transposition complexes

Country Status (4)

Country Link
US (1) US20100173800A1 (en)
EP (1) EP2171069A4 (en)
FI (1) FI20075520A0 (en)
WO (1) WO2009007503A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021041922A1 (en) * 2019-08-30 2021-03-04 The Broad Institute, Inc. Crispr-associated mu transposase systems

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001503962A (en) * 1996-02-09 2001-03-27 ヘット ネーデルランツ カンケル インスティチュート Vectors and methods for imparting additional nucleic acid material to cells integrated into the genome of the cell
US20020132350A1 (en) * 2000-09-14 2002-09-19 Pioneer Hi-Bred International, Inc. Targeted genetic manipulation using Mu bacteriophage cleaved donor complex
FI20030561A0 (en) * 2003-04-14 2003-04-14 Finnzymes Oy A method for transferring nucleic acids into eukaryotic genomes

Also Published As

Publication number Publication date
US20100173800A1 (en) 2010-07-08
EP2171069A1 (en) 2010-04-07
EP2171069A4 (en) 2011-01-05
WO2009007503A1 (en) 2009-01-15

Similar Documents

Publication Publication Date Title
HK1217106A1 (en) Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis dna
EP2132334A4 (en) Methods and compositions related to nucleic acid detection
ZA201102252B (en) Methods and kits for nucleic acid sequencing
DK3495498T3 (en) GENE EXPRESSION ANALYSIS IN SINGLE CELLS
DK2574677T3 (en) Methods of cell culture
ZA201003126B (en) Method and kit for preparation of sample for use in nucleic acid amplification
EP2201021A4 (en) Alternative nucleic acid sequencing methods
EP1987162A4 (en) Nucleic acid analysis using sequence tokens
GB0701253D0 (en) Nucleic acid amplification and testing
HK1149298A1 (en) Methods for nucleic acid sequencing
EP2121956A4 (en) Methods and compositions for nucleic acid amplification
PT2240572E (en) Use of rna for reprogramming somatic cells
EP2464755A4 (en) Methods and kits for 3'-end-tagging of rna
EP2207896A4 (en) Nucleic acid amplification
IL201420A0 (en) Nucleic acid enzymes and complexes and methods for their use
BRPI0817199A2 (en) IDENTIFICATIONB AND INSULATION OF FETAL CELLS AND NUCLEIC ACID
ATE510923T1 (en) NUCLEIC ACID
IL202090A0 (en) METHODS OF IDENTIFYING GENES INVOLVED IN MEMORY FORMATION USING SMALL INTERFERING RNA(siRNA)
HK1136849A1 (en) Methods and compositions for expressing negative-sense viral rna in canine cells rna
EP2337867A4 (en) Detection of npm1 nucleic acid in acellular body fluids
HK1193436A1 (en) Nucleic acid sample preparation methods and compositions
EP2235214B1 (en) Compositions and methods to detect candida albicans nucleic acid
EP2455469A4 (en) Nucleic acid molecule capable of binding to hmgb1, and use thereof
EP2362909A4 (en) Mammalian genes involved in infection
EP2118297A4 (en) Generation of nucleic acid molecules