DE602004029875D1 - Zuführung von nukleinsäuren in eukaryontische genome unter verwendung in vitro zusammengesetzter mu-transpositionskomplexe - Google Patents

Zuführung von nukleinsäuren in eukaryontische genome unter verwendung in vitro zusammengesetzter mu-transpositionskomplexe

Info

Publication number
DE602004029875D1
DE602004029875D1 DE602004029875T DE602004029875T DE602004029875D1 DE 602004029875 D1 DE602004029875 D1 DE 602004029875D1 DE 602004029875 T DE602004029875 T DE 602004029875T DE 602004029875 T DE602004029875 T DE 602004029875T DE 602004029875 D1 DE602004029875 D1 DE 602004029875D1
Authority
DE
Germany
Prior art keywords
transposition
eukaryontic
genome
introduction
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE602004029875T
Other languages
English (en)
Inventor
Harri Savilahti
Mikko Frilander
Meng Xiaojuan
Anja Paatero
Maria Pajunen
Hilkka Turakainen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Thermo Fisher Scientific Oy
Original Assignee
Finnzymes Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Finnzymes Oy filed Critical Finnzymes Oy
Publication of DE602004029875D1 publication Critical patent/DE602004029875D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE602004029875T 2003-04-14 2004-04-14 Zuführung von nukleinsäuren in eukaryontische genome unter verwendung in vitro zusammengesetzter mu-transpositionskomplexe Expired - Lifetime DE602004029875D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20030561A FI20030561A0 (fi) 2003-04-14 2003-04-14 Menetelmä nukleiinihappojen siirtämiseksi eukaryoottigenomeihin
PCT/FI2004/000228 WO2004090146A1 (en) 2003-04-14 2004-04-14 Delivery of nucleic acids into eukaryotic genomes using in vitro assembled mu transposition complexes

Publications (1)

Publication Number Publication Date
DE602004029875D1 true DE602004029875D1 (de) 2010-12-16

Family

ID=8565964

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004029875T Expired - Lifetime DE602004029875D1 (de) 2003-04-14 2004-04-14 Zuführung von nukleinsäuren in eukaryontische genome unter verwendung in vitro zusammengesetzter mu-transpositionskomplexe

Country Status (8)

Country Link
US (2) US8026052B1 (de)
EP (1) EP1613758B1 (de)
JP (1) JP4809213B2 (de)
AT (1) ATE486947T1 (de)
CA (1) CA2522438C (de)
DE (1) DE602004029875D1 (de)
FI (1) FI20030561A0 (de)
WO (1) WO2004090146A1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI20030561A0 (fi) * 2003-04-14 2003-04-14 Finnzymes Oy Menetelmä nukleiinihappojen siirtämiseksi eukaryoottigenomeihin
FI20075520A0 (fi) * 2007-07-06 2007-07-06 Finnzymes Oy Nukleiinihappojen siirtäminen ihmisen katansolujen genomeihin käyttäen in vitro -koottuja Mu-transpositiokomplekseja
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
EP3003994B1 (de) 2013-06-04 2023-07-05 Nordic Aquafarms AS Behälter, behälterserien und verfahren zur behandlung einer flüssigkeit
WO2017189894A1 (en) * 2016-04-27 2017-11-02 Yale University Multiplex genome engineering in eukaryotes
JP2023502317A (ja) * 2019-10-10 2023-01-24 カールスバーグ アグシャセルスガーブ 変異体植物の調製方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2627508B1 (fr) * 1988-02-22 1990-10-05 Eurolysine Procede pour l'integration d'un gene choisi sur le chromosome d'une bacterie et bacterie obtenue par ledit procede
AU7321294A (en) * 1993-06-30 1995-01-24 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College, The Transformed eukaryotic cells, and transposon-based transformation vectors
US5677170A (en) * 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
US6159736A (en) 1998-09-23 2000-12-12 Wisconsin Alumni Research Foundation Method for making insertional mutations using a Tn5 synaptic complex
GB9917531D0 (en) * 1999-07-26 1999-09-29 Arrow Therapeutics Ltd Transposon mediated differential hybridisation
JP3451311B2 (ja) * 2000-02-28 2003-09-29 独立行政法人産業技術総合研究所 染色体組込み用ｄｎａ断片、ベクター及びそれらを用いた染色体への遺伝子導入方法
US20020094575A1 (en) * 2000-09-14 2002-07-18 Pioneer Hi-Bred International, Inc. Compositions and methods for stable transformation using Mu bacteriophage cleaved donor complex
US20020132350A1 (en) * 2000-09-14 2002-09-19 Pioneer Hi-Bred International, Inc. Targeted genetic manipulation using Mu bacteriophage cleaved donor complex
US20030143740A1 (en) 2001-10-15 2003-07-31 Christine Wooddell Processes for transposase mediated integration into mammalian cells
WO2003054151A2 (en) * 2001-12-11 2003-07-03 Van Andel Institute Generation of transgenic mice by transgene-mediated rescue of spermatogenesis
FI20030561A0 (fi) * 2003-04-14 2003-04-14 Finnzymes Oy Menetelmä nukleiinihappojen siirtämiseksi eukaryoottigenomeihin

Also Published As

Publication number Publication date
US8192934B2 (en) 2012-06-05
CA2522438A1 (en) 2004-10-21
WO2004090146A1 (en) 2004-10-21
FI20030561A0 (fi) 2003-04-14
JP4809213B2 (ja) 2011-11-09
EP1613758A1 (de) 2006-01-11
CA2522438C (en) 2012-09-25
ATE486947T1 (de) 2010-11-15
US20120015831A1 (en) 2012-01-19
US8026052B1 (en) 2011-09-27
EP1613758B1 (de) 2010-11-03
JP2006522596A (ja) 2006-10-05

Similar Documents

Publication Publication Date Title
Chung et al. The use of duplex-specific nuclease in ribosome profiling and a user-friendly software package for Ribo-seq data analysis
CN106604994B (zh) 通过测序评估的DSBs的全基因组无偏鉴定(GUIDE-Seq)
EP3469084A1 (de) Hochspezifische genomeditierung durch chemisch modifizierte guide-rnas
Anderson et al. BglBricks: A flexible standard for biological part assembly
US11275081B2 (en) Pumilio domain-based modular protein architecture for RNA binding
US20180245071A1 (en) Comprehensive In vitro Reporting of Cleavage Events by Sequencing (CIRCLE-seq)
WO2018081535A2 (en) Dynamic genome engineering
CN111201329A (zh) 具有减少的扩增偏倚的高通量单细胞测序
Arazoe et al. Targeted nucleotide editing technologies for microbial metabolic engineering
Trubitsyna et al. PaperClip: rapid multi-part DNA assembly from existing libraries
Si et al. Rapid prototyping of microbial cell factories via genome-scale engineering
Doi et al. Catalytically inactive Cas9 impairs DNA replication fork progression to induce focal genomic instability
Tsvetanova et al. Genetic assembly tools for synthetic biology
Tang et al. Efficient cleavage resolves PAM preferences of CRISPR-Cas in human cells
Schindler et al. Synthetic chromosomes
DE602004029875D1 (de) Zuführung von nukleinsäuren in eukaryontische genome unter verwendung in vitro zusammengesetzter mu-transpositionskomplexe
Hand et al. Directed evolution studies of a thermophilic Type II-C Cas9
Zeng et al. Advanced genome-editing technologies enable rapid and large-scale generation of genetic variants for strain engineering and synthetic biology
Pryor et al. Rapid 40 kb genome construction from 52 parts
Sakata et al. A single CRISPR base editor to induce simultaneous C-to-T and A-to-G mutations
Weisbach et al. Multiplexed genome engineering with Cas12a
Li et al. Enabling technologies in synthetic biology—DNA synthesis, assembly and editing
Miller et al. A novel, single-tube enzymatic fragmentation and library construction method enables fast turnaround times and improved data quality for microbial whole-genome sequencing
Qi et al. Utilizing Epigenetic Modification as a Reactive Handle To Regulate RNA Function and CRISPR-Based Gene Regulation
Lyu et al. LAST-seq: single-cell RNA sequencing by direct amplification of single-stranded RNA without prior reverse transcription and second-strand synthesis