KR101981662B1 - 형질전환된 미생물 예컨대 피치아 패스토리스에서 다중-서브유닛 단백질 예컨대 항체의 고순도 생산 - Google Patents

형질전환된 미생물 예컨대 피치아 패스토리스에서 다중-서브유닛 단백질 예컨대 항체의 고순도 생산 Download PDF

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KR101981662B1
KR101981662B1 KR1020137033842A KR20137033842A KR101981662B1 KR 101981662 B1 KR101981662 B1 KR 101981662B1 KR 1020137033842 A KR1020137033842 A KR 1020137033842A KR 20137033842 A KR20137033842 A KR 20137033842A KR 101981662 B1 KR101981662 B1 KR 101981662B1
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seq
delete delete
antibody
polypeptide
ngf
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KR20140033445A (ko
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패트리샤 다이앤 맥닐
니콜 잰슨
그레이 엘. 레스니키
페이 퀴
존 에이. 래썸
리언 에프. 가르시아-마르티네즈
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앨더바이오 홀딩스 엘엘씨
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

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  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
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  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
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  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
KR1020137033842A 2011-05-20 2012-05-08 형질전환된 미생물 예컨대 피치아 패스토리스에서 다중-서브유닛 단백질 예컨대 항체의 고순도 생산 Active KR101981662B1 (ko)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201161488660P 2011-05-20 2011-05-20
US61/488,660 2011-05-20
US201161496873P 2011-06-14 2011-06-14
US201161496860P 2011-06-14 2011-06-14
US61/496,873 2011-06-14
US61/496,860 2011-06-14
US201161525307P 2011-08-19 2011-08-19
US61/525,307 2011-08-19
PCT/US2012/036953 WO2012161956A2 (en) 2011-05-20 2012-05-08 High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris

Publications (2)

Publication Number Publication Date
KR20140033445A KR20140033445A (ko) 2014-03-18
KR101981662B1 true KR101981662B1 (ko) 2019-05-23

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KR1020137033842A Active KR101981662B1 (ko) 2011-05-20 2012-05-08 형질전환된 미생물 예컨대 피치아 패스토리스에서 다중-서브유닛 단백질 예컨대 항체의 고순도 생산

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Country Link
EP (1) EP2710114B1 (https=)
JP (2) JP6193220B2 (https=)
KR (1) KR101981662B1 (https=)
CN (2) CN103827290A (https=)
CA (1) CA2837000C (https=)
CY (1) CY1124796T1 (https=)
DK (1) DK2710114T3 (https=)
EA (1) EA028913B9 (https=)
ES (1) ES2899381T3 (https=)
HR (1) HRP20211873T1 (https=)
HU (1) HUE060418T2 (https=)
IL (1) IL229430B (https=)
LT (1) LT2710114T (https=)
MX (2) MX368546B (https=)
PL (1) PL2710114T3 (https=)
PT (1) PT2710114T (https=)
RS (1) RS62628B1 (https=)
SG (1) SG194937A1 (https=)
SI (1) SI2710114T1 (https=)
SM (1) SMT202100677T1 (https=)
WO (1) WO2012161956A2 (https=)

Families Citing this family (5)

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Publication number Priority date Publication date Assignee Title
PE20161439A1 (es) * 2014-03-21 2017-01-26 Teva Pharmaceuticals Int Gmbh Anticuerpos antagonistas dirigidos contra el peptido relacionado con el gen de calcitonina y metodos que usan los mismos
TWI734775B (zh) 2016-04-26 2021-08-01 美商美國泰福生技股份有限公司 細胞培養基
WO2018055574A1 (en) 2016-09-23 2018-03-29 Teva Pharmaceuticals International Gmbh Treating refractory migraine
GB201803832D0 (en) * 2018-03-09 2018-04-25 Syndermix Ag Methods and compositions for lectin production
CN108913732B (zh) * 2018-07-31 2022-02-08 华东理工大学 一种莫纳可林j异源生产的方法及应用

Citations (2)

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WO2000056903A2 (en) * 1999-03-22 2000-09-28 Zymogenetics, Inc. IMPROVED METHODS FOR PRODUCING PROTEINS IN TRANSFORMED $i(PICHIA)
WO2008063302A2 (en) * 2006-10-10 2008-05-29 Keck Graduate Institute Novel p. pastoris pastoris promoters, and the use thereof to direct expression of proteins in yeast, preferably using a haploid mating strategy

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US4855231A (en) 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
US4818700A (en) 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
US4812405A (en) 1986-02-18 1989-03-14 Phillips Petroleum Company Double auxotrophic mutants of Pichia pastoris and methods for preparation
US4929555A (en) 1987-10-19 1990-05-29 Phillips Petroleum Company Pichia transformation
WO1994021293A1 (en) 1993-03-19 1994-09-29 Duke University Method of treatment of tumors with an antibody binding to tenascin
ES2251723T3 (es) 1994-08-12 2006-05-01 Immunomedics, Inc. Inmunoconjugados y anticuerpos humanizados especificos para linfoma de celulas b y celulas de leucemia.
US5955349A (en) 1996-08-26 1999-09-21 Zymogenetics, Inc. Compositions and methods for producing heterologous polypeptides in Pichia methanolica
US5736383A (en) 1996-08-26 1998-04-07 Zymogenetics, Inc. Preparation of Pichia methanolica auxotrophic mutants
US6653104B2 (en) 1996-10-17 2003-11-25 Immunomedics, Inc. Immunotoxins, comprising an internalizing antibody, directed against malignant and normal cells
US6730499B1 (en) 1998-07-03 2004-05-04 Research Corporation Technologies, Inc. Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1
US6258559B1 (en) 1999-03-22 2001-07-10 Zymogenetics, Inc. Method for producing proteins in transformed Pichia
BR0116675A (pt) * 2000-12-13 2003-11-04 Unilever Nv Método de produção de uma proteìna heteróloga por um fungo e proteìna heteróloga
PT1678314E (pt) 2003-10-22 2012-11-27 Keck Graduate Inst Métodos de síntese de polipéptidos heteromultiméricos em levedura utilizando uma estratégia de conjugação haplóide
HUE032563T2 (en) 2007-05-21 2017-09-28 Alderbio Holdings Llc Antibodies to IL-6 and their use
KR20170036814A (ko) 2007-05-21 2017-04-03 앨더바이오 홀딩스 엘엘씨 신규한 래빗 항체 인간화 방법 및 인간화된 래빗 항체
JP6190723B2 (ja) * 2010-12-01 2017-08-30 アルダーバイオ ホールディングス エルエルシー 抗ｎｇｆ組成物およびその使用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000056903A2 (en) * 1999-03-22 2000-09-28 Zymogenetics, Inc. IMPROVED METHODS FOR PRODUCING PROTEINS IN TRANSFORMED $i(PICHIA)
WO2008063302A2 (en) * 2006-10-10 2008-05-29 Keck Graduate Institute Novel p. pastoris pastoris promoters, and the use thereof to direct expression of proteins in yeast, preferably using a haploid mating strategy

Also Published As

Publication number Publication date
KR20140033445A (ko) 2014-03-18
EA028913B9 (ru) 2018-03-30
RS62628B1 (sr) 2021-12-31
DK2710114T3 (da) 2021-12-06
CA2837000C (en) 2021-09-14
IL229430B (en) 2020-07-30
CN103827290A (zh) 2014-05-28
HRP20211873T1 (hr) 2022-03-04
EP2710114B1 (en) 2021-11-03
EP2710114A4 (en) 2014-11-19
WO2012161956A2 (en) 2012-11-29
EP2710114A2 (en) 2014-03-26
MX2019012022A (es) 2019-11-18
WO2012161956A9 (en) 2013-09-06
SG194937A1 (en) 2013-12-30
WO2012161956A3 (en) 2013-01-17
SI2710114T1 (sl) 2022-01-31
MX2013013532A (es) 2015-08-20
EA201301294A1 (ru) 2014-05-30
CN110408644A (zh) 2019-11-05
IL229430A0 (en) 2014-01-30
JP6491696B2 (ja) 2019-03-27
PT2710114T (pt) 2021-12-09
CY1124796T1 (el) 2022-11-25
CA2837000A1 (en) 2012-11-29
SMT202100677T1 (it) 2022-01-10
JP2018007656A (ja) 2018-01-18
PL2710114T3 (pl) 2022-01-17
JP2014517698A (ja) 2014-07-24
HUE060418T2 (hu) 2023-02-28
NZ618635A (en) 2016-09-30
ES2899381T3 (es) 2022-03-11
BR112013029902A2 (pt) 2016-12-20
NZ718187A (en) 2017-10-27
LT2710114T (lt) 2021-12-10
EA028913B1 (ru) 2018-01-31
JP6193220B2 (ja) 2017-09-06
MX368546B (es) 2019-10-07

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