HRP20211873T1 - Proizvodnja visoke čistoće proteina s više podjedinica kao što su protutijela u transformiranim mikrobima kao što je pichia pastoris - Google Patents
Proizvodnja visoke čistoće proteina s više podjedinica kao što su protutijela u transformiranim mikrobima kao što je pichia pastoris Download PDFInfo
- Publication number
- HRP20211873T1 HRP20211873T1 HRP20211873TT HRP20211873T HRP20211873T1 HR P20211873 T1 HRP20211873 T1 HR P20211873T1 HR P20211873T T HRP20211873T T HR P20211873TT HR P20211873 T HRP20211873 T HR P20211873T HR P20211873 T1 HRP20211873 T1 HR P20211873T1
- Authority
- HR
- Croatia
- Prior art keywords
- antibody
- culture
- seq
- nos
- cdr sequences
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161488660P | 2011-05-20 | 2011-05-20 | |
| US201161496873P | 2011-06-14 | 2011-06-14 | |
| US201161496860P | 2011-06-14 | 2011-06-14 | |
| US201161525307P | 2011-08-19 | 2011-08-19 | |
| EP12789187.7A EP2710114B1 (en) | 2011-05-20 | 2012-05-08 | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris |
| PCT/US2012/036953 WO2012161956A2 (en) | 2011-05-20 | 2012-05-08 | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20211873T1 true HRP20211873T1 (hr) | 2022-03-04 |
Family
ID=47217961
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20211873TT HRP20211873T1 (hr) | 2011-05-20 | 2012-05-08 | Proizvodnja visoke čistoće proteina s više podjedinica kao što su protutijela u transformiranim mikrobima kao što je pichia pastoris |
Country Status (21)
| Country | Link |
|---|---|
| EP (1) | EP2710114B1 (https=) |
| JP (2) | JP6193220B2 (https=) |
| KR (1) | KR101981662B1 (https=) |
| CN (2) | CN103827290A (https=) |
| CA (1) | CA2837000C (https=) |
| CY (1) | CY1124796T1 (https=) |
| DK (1) | DK2710114T3 (https=) |
| EA (1) | EA028913B9 (https=) |
| ES (1) | ES2899381T3 (https=) |
| HR (1) | HRP20211873T1 (https=) |
| HU (1) | HUE060418T2 (https=) |
| IL (1) | IL229430B (https=) |
| LT (1) | LT2710114T (https=) |
| MX (2) | MX368546B (https=) |
| PL (1) | PL2710114T3 (https=) |
| PT (1) | PT2710114T (https=) |
| RS (1) | RS62628B1 (https=) |
| SG (1) | SG194937A1 (https=) |
| SI (1) | SI2710114T1 (https=) |
| SM (1) | SMT202100677T1 (https=) |
| WO (1) | WO2012161956A2 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PE20161439A1 (es) * | 2014-03-21 | 2017-01-26 | Teva Pharmaceuticals Int Gmbh | Anticuerpos antagonistas dirigidos contra el peptido relacionado con el gen de calcitonina y metodos que usan los mismos |
| TWI734775B (zh) | 2016-04-26 | 2021-08-01 | 美商美國泰福生技股份有限公司 | 細胞培養基 |
| WO2018055574A1 (en) | 2016-09-23 | 2018-03-29 | Teva Pharmaceuticals International Gmbh | Treating refractory migraine |
| GB201803832D0 (en) * | 2018-03-09 | 2018-04-25 | Syndermix Ag | Methods and compositions for lectin production |
| CN108913732B (zh) * | 2018-07-31 | 2022-02-08 | 华东理工大学 | 一种莫纳可林j异源生产的方法及应用 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| US4812405A (en) | 1986-02-18 | 1989-03-14 | Phillips Petroleum Company | Double auxotrophic mutants of Pichia pastoris and methods for preparation |
| US4929555A (en) | 1987-10-19 | 1990-05-29 | Phillips Petroleum Company | Pichia transformation |
| WO1994021293A1 (en) | 1993-03-19 | 1994-09-29 | Duke University | Method of treatment of tumors with an antibody binding to tenascin |
| ES2251723T3 (es) | 1994-08-12 | 2006-05-01 | Immunomedics, Inc. | Inmunoconjugados y anticuerpos humanizados especificos para linfoma de celulas b y celulas de leucemia. |
| US5955349A (en) | 1996-08-26 | 1999-09-21 | Zymogenetics, Inc. | Compositions and methods for producing heterologous polypeptides in Pichia methanolica |
| US5736383A (en) | 1996-08-26 | 1998-04-07 | Zymogenetics, Inc. | Preparation of Pichia methanolica auxotrophic mutants |
| US6653104B2 (en) | 1996-10-17 | 2003-11-25 | Immunomedics, Inc. | Immunotoxins, comprising an internalizing antibody, directed against malignant and normal cells |
| US6730499B1 (en) | 1998-07-03 | 2004-05-04 | Research Corporation Technologies, Inc. | Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1 |
| US6258559B1 (en) | 1999-03-22 | 2001-07-10 | Zymogenetics, Inc. | Method for producing proteins in transformed Pichia |
| AU3910000A (en) * | 1999-03-22 | 2000-10-09 | Zymogenetics Inc. | Improved methods for producing proteins in transformed (pichia) |
| BR0116675A (pt) * | 2000-12-13 | 2003-11-04 | Unilever Nv | Método de produção de uma proteìna heteróloga por um fungo e proteìna heteróloga |
| PT1678314E (pt) | 2003-10-22 | 2012-11-27 | Keck Graduate Inst | Métodos de síntese de polipéptidos heteromultiméricos em levedura utilizando uma estratégia de conjugação haplóide |
| US8222386B2 (en) | 2006-10-10 | 2012-07-17 | Keck Graduate Institute | P. pastoris ADH promoter and use thereof to direct expression of proteins |
| HUE032563T2 (en) | 2007-05-21 | 2017-09-28 | Alderbio Holdings Llc | Antibodies to IL-6 and their use |
| KR20170036814A (ko) | 2007-05-21 | 2017-04-03 | 앨더바이오 홀딩스 엘엘씨 | 신규한 래빗 항체 인간화 방법 및 인간화된 래빗 항체 |
| JP6190723B2 (ja) * | 2010-12-01 | 2017-08-30 | アルダーバイオ ホールディングス エルエルシー | 抗ｎｇｆ組成物およびその使用 |
-
2012
- 2012-05-08 DK DK12789187.7T patent/DK2710114T3/da active
- 2012-05-08 SG SG2013084041A patent/SG194937A1/en unknown
- 2012-05-08 HU HUE12789187A patent/HUE060418T2/hu unknown
- 2012-05-08 WO PCT/US2012/036953 patent/WO2012161956A2/en not_active Ceased
- 2012-05-08 JP JP2014512858A patent/JP6193220B2/ja active Active
- 2012-05-08 CA CA2837000A patent/CA2837000C/en active Active
- 2012-05-08 SI SI201231967T patent/SI2710114T1/sl unknown
- 2012-05-08 CN CN201280035868.4A patent/CN103827290A/zh active Pending
- 2012-05-08 CN CN201910566412.4A patent/CN110408644A/zh active Pending
- 2012-05-08 LT LTEPPCT/US2012/036953T patent/LT2710114T/lt unknown
- 2012-05-08 EA EA201301294A patent/EA028913B9/ru not_active IP Right Cessation
- 2012-05-08 RS RS20211462A patent/RS62628B1/sr unknown
- 2012-05-08 MX MX2013013532A patent/MX368546B/es active IP Right Grant
- 2012-05-08 ES ES12789187T patent/ES2899381T3/es active Active
- 2012-05-08 EP EP12789187.7A patent/EP2710114B1/en active Active
- 2012-05-08 KR KR1020137033842A patent/KR101981662B1/ko active Active
- 2012-05-08 PT PT127891877T patent/PT2710114T/pt unknown
- 2012-05-08 PL PL12789187T patent/PL2710114T3/pl unknown
- 2012-05-08 SM SM20210677T patent/SMT202100677T1/it unknown
- 2012-05-08 HR HRP20211873TT patent/HRP20211873T1/hr unknown
-
2013
- 2013-11-14 IL IL229430A patent/IL229430B/en active IP Right Grant
- 2013-11-19 MX MX2019012022A patent/MX2019012022A/es unknown
-
2017
- 2017-06-19 JP JP2017119537A patent/JP6491696B2/ja active Active
-
2021
- 2021-12-02 CY CY20211101055T patent/CY1124796T1/el unknown
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2905180C (en) | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells | |
| AU2017255538B2 (en) | Cell culture medium | |
| HRP20211873T1 (hr) | Proizvodnja visoke čistoće proteina s više podjedinica kao što su protutijela u transformiranim mikrobima kao što je pichia pastoris | |
| TWI666318B (zh) | 在經轉形的微生物如巴斯德畢赤氏酵母中的多－複製策略用於多－次級單元的蛋白質如抗體之高效價且高純度生產技術 | |
| US11225667B2 (en) | Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris | |
| US9802993B2 (en) | Method for producing a cell for protein production by treating a cell overexpressing a taurine transporter with methotrexate | |
| US12077794B2 (en) | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells | |
| CN117802022B (zh) | 一种产四氢嘧啶的基因工程菌及其构建方法与应用 | |
| KR101318498B1 (ko) | 재조합 단백질의 생산성 향상을 위한 배양용 배지 첨가용 조성물 | |
| Massahi | Effect of signal sequences and promoters in recombinant extracellular protein production by Pichia pastoris | |
| Ningaraju et al. | Pichia pastoris-A Model System for Expression of Recombinant Proteins | |
| HK40020901A (en) | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells | |
| EA014422B1 (ru) | ШТАММ ДРОЖЖЕЙ PICHIA PASTORIS PS107(pPIC9HAbIL-2), ЯВЛЯЮЩИЙСЯ ПРОДУЦЕНТОМ ГИБРИДНОГО БЕЛКА, СОСТОЯЩЕГО ИЗ АЛЬБУМИНА ПЛАЗМЫ КРОВИ ЧЕЛОВЕКА И ИНТЕРЛЕЙКИНА-2 ЧЕЛОВЕКА, РЕКОМБИНАНТНАЯ ПЛАЗМИДА pPIC9HAbIL-2 И СПОСОБ ЕЕ КОНСТРУИРОВАНИЯ | |
| Khatri | Optimisation of recombinant protein production in Pichia pastoris: Single-chain antibody fragment model protein | |
| Ojito Magaz et al. | Development of a Fed-Batch Strategy for the Production of NS0-Derived Humanised Mab in Protein Free Medium | |
| NZ711400B2 (en) | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells |