KR101791597B1 - 모그로사이드 화합물을 효소에 의해 합성하기 위한 방법 및 재료 - Google Patents

모그로사이드 화합물을 효소에 의해 합성하기 위한 방법 및 재료 Download PDF

Info

Publication number
KR101791597B1
KR101791597B1 KR1020147016910A KR20147016910A KR101791597B1 KR 101791597 B1 KR101791597 B1 KR 101791597B1 KR 1020147016910 A KR1020147016910 A KR 1020147016910A KR 20147016910 A KR20147016910 A KR 20147016910A KR 101791597 B1 KR101791597 B1 KR 101791597B1
Authority
KR
South Korea
Prior art keywords
leu
glu
val
gly
ile
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020147016910A
Other languages
English (en)
Korean (ko)
Other versions
KR20140099281A (ko
Inventor
야오콴 리우
정 엽 이
모니카 카레
Original Assignee
에볼바 에스아
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 에볼바 에스아 filed Critical 에볼바 에스아
Publication of KR20140099281A publication Critical patent/KR20140099281A/ko
Application granted granted Critical
Publication of KR101791597B1 publication Critical patent/KR101791597B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • C12P33/20—Preparation of steroids containing heterocyclic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020147016910A 2011-11-23 2012-11-19 모그로사이드 화합물을 효소에 의해 합성하기 위한 방법 및 재료 Active KR101791597B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161563303P 2011-11-23 2011-11-23
US61/563,303 2011-11-23
PCT/IB2012/002857 WO2013076577A1 (en) 2011-11-23 2012-11-19 Methods and materials for enzymatic synthesis of mogroside compounds

Publications (2)

Publication Number Publication Date
KR20140099281A KR20140099281A (ko) 2014-08-11
KR101791597B1 true KR101791597B1 (ko) 2017-10-30

Family

ID=47624376

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020147016910A Active KR101791597B1 (ko) 2011-11-23 2012-11-19 모그로사이드 화합물을 효소에 의해 합성하기 위한 방법 및 재료

Country Status (14)

Country Link
US (2) US9920349B2 (enExample)
EP (1) EP2783009B1 (enExample)
JP (1) JP6084983B2 (enExample)
KR (1) KR101791597B1 (enExample)
CN (1) CN103958693B (enExample)
AU (2) AU2012342114B2 (enExample)
BR (1) BR112014012543A2 (enExample)
CA (1) CA2853677C (enExample)
IN (1) IN2014CN03557A (enExample)
MX (1) MX342851B (enExample)
MY (1) MY170686A (enExample)
RU (1) RU2633644C2 (enExample)
SG (1) SG11201401927PA (enExample)
WO (1) WO2013076577A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210050044A (ko) * 2019-10-25 2021-05-07 고려대학교 산학협력단 테르펜 당쇄 부가 유도체, 이의 제조방법 및 이를 유효성분으로 함유하는 조성물
US12234464B2 (en) 2018-11-09 2025-02-25 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR122021005297B1 (pt) 2010-06-02 2022-02-22 Evolva, Inc Método para transferir uma segunda porção de açúcar para uma posição c-2' de uma glicose em um glicosídeo de esteviol
JP6262653B2 (ja) 2011-08-08 2018-01-17 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． ステビオール配糖体の組換え生産
AU2012342114B2 (en) 2011-11-23 2017-12-21 Danstar Ferment Ag Methods and materials for enzymatic synthesis of mogroside compounds
TW201402599A (zh) * 2012-05-30 2014-01-16 Suntory Holdings Ltd 甜菊糖苷糖苷轉化酵素及編碼該酵素之基因
WO2016050890A2 (en) 2014-10-01 2016-04-07 Evolva Sa Methods and materials for biosynthesis of mogroside compounds
US9932619B2 (en) * 2012-12-04 2018-04-03 Evolva Sa Methods and materials for biosynthesis of mogroside compounds
CN105189771B (zh) 2013-02-11 2019-11-05 埃沃尔瓦公司 在重组宿主中有效产生甜菊醇糖苷
CA2957331A1 (en) 2014-08-11 2016-02-18 Evolva Sa Production of steviol glycosides in recombinant hosts
US10612064B2 (en) 2014-09-09 2020-04-07 Evolva Sa Production of steviol glycosides in recombinant hosts
EP3191584A4 (en) 2014-09-11 2018-07-18 The State of Israel - Ministry of Agriculture & Rural Development, Agricultural Research Organization (ARO) (Volcani Center) Methods of producing mogrosides and compositions comprising same and uses thereof
EP3208342A4 (en) * 2014-10-17 2018-07-04 Suntory Holdings Limited Mogrol glycosyltransferase and gene encoding same
CA2974185A1 (en) 2015-01-20 2016-07-28 Suntory Holdings Limited Method for preparing mogroside
EP4148137A1 (en) 2015-01-30 2023-03-15 Evolva SA Production of steviol glycosides in recombinant hosts
CA2979931A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
BR112018002564A2 (pt) 2015-08-07 2018-09-25 Evolva Sa produção de glicosídeos de esteviol em hospedeiros recombinantes
JP6949838B2 (ja) 2015-10-29 2021-10-13 フィルメニッヒ インコーポレイテッドＦｉｒｍｅｎｉｃｈ Ｉｎｃｏｒｐｏｒａｔｅｄ 高甘味度甘味料
CN106924274B (zh) * 2015-12-29 2019-01-04 成都普睿法药物研发有限公司 葫芦烷型四环三萜类化合物抗肺纤维化应用
CN109195457A (zh) 2016-04-13 2019-01-11 埃沃尔瓦公司 在重组宿主中产生甜菊醇糖苷
US10815514B2 (en) 2016-05-16 2020-10-27 Evolva Sa Production of steviol glycosides in recombinant hosts
WO2017198681A1 (en) * 2016-05-16 2017-11-23 Evolva Sa Production of steviol glycosides in recombinant hosts
CN109477126B (zh) 2016-07-19 2023-06-20 三得利控股株式会社 罗汉果醇或罗汉果醇糖苷的生产方法
CN110100006A (zh) 2016-11-07 2019-08-06 埃沃尔瓦公司 重组宿主中甜菊糖苷的生产
JP7285786B2 (ja) 2017-05-03 2023-06-02 フィルメニッヒ インコーポレイテッド 高強度の甘味料の製造方法
JP7285788B2 (ja) 2017-05-19 2023-06-02 ザ コカ・コーラ カンパニー シアメノシドｉ甘味料入り組成物及びその使用
CN116941752A (zh) 2017-06-02 2023-10-27 奇华顿股份有限公司 组合物
WO2018229283A1 (en) 2017-06-15 2018-12-20 Evolva Sa Production of mogroside compounds in recombinant hosts
CN107189976B (zh) * 2017-07-28 2022-03-11 李华政 促进罗汉果ugt72b21基因表达的方法
JP2022512931A (ja) 2018-11-07 2022-02-07 フィルメニッヒ インコーポレイテッド 高甘味度甘味料の製造方法
US12286661B2 (en) 2018-11-07 2025-04-29 Firmenich Incorporated Methods for making high intensity sweeteners
CN110669094B (zh) * 2019-10-14 2021-12-21 上海交通大学 罗汉果新苷及其制备方法
MX2022011627A (es) * 2020-03-17 2022-12-15 Coca Cola Co Nuevo sistema y métodos de producción de mogrósidos.
CA3179775A1 (en) 2020-04-20 2021-10-28 Givaudan Sa Compositions
GB202007984D0 (en) 2020-05-28 2020-07-15 Givaudan Sa Compositions
CN112063647B (zh) * 2020-09-17 2023-05-02 云南农业大学 酿酒酵母重组菌Cuol01的构建方法、酿酒酵母重组菌Cuol02及应用
CN116064712B (zh) * 2022-10-08 2025-05-06 中国药科大学 一种罗汉果醇的生物合成方法
CN121127603A (zh) 2023-04-20 2025-12-12 帝斯曼知识产权资产管理有限公司 用于产生罗汉果苷的菌株和方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008065370A2 (en) 2006-12-01 2008-06-05 The University Of York Sesquiterpenoid modifying enzymes

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6399321B1 (en) * 1999-08-18 2002-06-04 National Research Council Of Canada Methods for screening UDP-glucose:glycoprotein glucosyltransferase (UGGT) activity and nucleic acid encoding for UGGT
EP1510573A1 (en) * 2003-09-01 2005-03-02 Austria Wirtschaftsservice Gesellschaft mit beschränkter Haftung Method for detoxification of mycotoxins
US20080148432A1 (en) * 2005-12-21 2008-06-19 Mark Scott Abad Transgenic plants with enhanced agronomic traits
AU2006219823A1 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
US8257948B1 (en) * 2011-02-17 2012-09-04 Purecircle Usa Method of preparing alpha-glucosyl Stevia composition
US8956677B2 (en) 2005-11-23 2015-02-17 The Coca-Cola Company High-potency sweetener composition with glucosamine and compositions sweetened therewith
RU2444900C2 (ru) * 2005-11-23 2012-03-20 Дзе Кока-Кола Компани Молочная композиция с интенсивным подсластителем
US7927851B2 (en) 2006-03-21 2011-04-19 Vineland Research And Innovation Centre Compositions having ent-kaurenoic acid 13-hydroxylase activity and methods for producing same
US20100143975A1 (en) 2006-11-22 2010-06-10 The University Of York Monoterpenoid modifying enzymes
GB0904639D0 (en) * 2009-03-18 2009-04-29 Univ York Assay
BR122021005297B1 (pt) 2010-06-02 2022-02-22 Evolva, Inc Método para transferir uma segunda porção de açúcar para uma posição c-2' de uma glicose em um glicosídeo de esteviol
AU2012342114B2 (en) 2011-11-23 2017-12-21 Danstar Ferment Ag Methods and materials for enzymatic synthesis of mogroside compounds
US9932619B2 (en) 2012-12-04 2018-04-03 Evolva Sa Methods and materials for biosynthesis of mogroside compounds

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008065370A2 (en) 2006-12-01 2008-06-05 The University Of York Sesquiterpenoid modifying enzymes

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12234464B2 (en) 2018-11-09 2025-02-25 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
KR20210050044A (ko) * 2019-10-25 2021-05-07 고려대학교 산학협력단 테르펜 당쇄 부가 유도체, 이의 제조방법 및 이를 유효성분으로 함유하는 조성물
KR102362135B1 (ko) * 2019-10-25 2022-02-14 고려대학교 산학협력단 테르펜 당쇄 부가 유도체, 이의 제조방법 및 이를 유효성분으로 함유하는 조성물

Also Published As

Publication number Publication date
US20140308698A1 (en) 2014-10-16
US20180237819A1 (en) 2018-08-23
CA2853677A1 (en) 2013-05-30
EP2783009A1 (en) 2014-10-01
JP6084983B2 (ja) 2017-02-22
CA2853677C (en) 2019-01-29
AU2017235907A1 (en) 2017-10-12
MX2014006276A (es) 2014-10-13
EP2783009B1 (en) 2016-04-13
BR112014012543A2 (pt) 2017-06-13
CN103958693A (zh) 2014-07-30
KR20140099281A (ko) 2014-08-11
SG11201401927PA (en) 2014-09-26
WO2013076577A1 (en) 2013-05-30
CN103958693B (zh) 2016-08-24
RU2014125216A (ru) 2015-12-27
US9920349B2 (en) 2018-03-20
MY170686A (en) 2019-08-26
AU2012342114B2 (en) 2017-12-21
RU2633644C2 (ru) 2017-10-16
HK1200494A1 (zh) 2015-08-07
JP2014533518A (ja) 2014-12-15
US10738340B2 (en) 2020-08-11
MX342851B (es) 2016-10-14
AU2012342114A1 (en) 2014-05-22
IN2014CN03557A (enExample) 2015-10-09

Similar Documents

Publication Publication Date Title
KR101791597B1 (ko) 모그로사이드 화합물을 효소에 의해 합성하기 위한 방법 및 재료
Hirade et al. Identification and functional analysis of 2-hydroxyflavanone C-glucosyltransferase in soybean (Glycine max)
US20250331481A1 (en) Group of udp-glycosyltransferase for catalyzing carbohydrate chain elongation, and application thereof
CN104357418B (zh) 一种糖基转移酶及其突变体在合成人参皂苷Rh2中的应用
CN105087739A (zh) 一种新的制备稀有人参皂苷的催化体系及其应用
CN113136373A (zh) 新型碳苷糖基转移酶及其应用
KR20210153670A (ko) 변이체 효소를 통한 스테비올 글리코사이드 레바우디오사이드 i의 생합성 생산
US20230105024A1 (en) Bifunctional C-Glycoside Glycosyltransferases and Application Thereof
WO2020048523A1 (zh) 合成黄芩素和野黄芩素的微生物、其制备方法及其应用
JP7749799B2 (ja) ラムノースの特異性の高い糖転移酵素及びその応用
Yang et al. Systems metabolic engineering of Escherichia coli to enhance the production of flavonoid glucuronides
CN108359652A (zh) 糖基转移酶及其应用
HK1200494B (en) Methods and materials for enzymatic synthesis of mogroside compounds
CN106520645A (zh) 绞股蓝糖基转移酶的工程菌及其构建方法与应用
CN113444703B (zh) 催化糖链延伸的糖基转移酶突变体及其应用
CN110218711B (zh) 一种酶蛋白及其编码基因与应用
CN119530188A (zh) 构建高效的糖基转移酶级联催化体系在合成新型三萜类糖苷中的应用
CN111926000A (zh) 绞股蓝糖基转移酶及其应用
KR20110029372A (ko) 산소-메틸화전이효소 변이체

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

A201 Request for examination
A302 Request for accelerated examination
P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

PA0302 Request for accelerated examination

St.27 status event code: A-1-2-D10-D17-exm-PA0302

St.27 status event code: A-1-2-D10-D16-exm-PA0302

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E90F Notification of reason for final refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

St.27 status event code: A-1-2-D10-D22-exm-PE0701

GRNT Written decision to grant
PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U12-oth-PR1002

Fee payment year number: 1

PG1601 Publication of registration

St.27 status event code: A-4-4-Q10-Q13-nap-PG1601

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 4

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 5

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 6

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 7

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 8

PN2301 Change of applicant

St.27 status event code: A-5-5-R10-R11-asn-PN2301

PN2301 Change of applicant

St.27 status event code: A-5-5-R10-R14-asn-PN2301

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 9